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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. problem with yeast transformation - (reply: 2)
1172. ta cloning of pfu amplified fragment - (reply: 9)
1173. how to express the gene in opposite transcriptional direction - (reply: 3)
1174. Water for electrocompetent cells - (reply: 2)
1175. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1176. Bacterial growth in agar plate with ampicillin - (reply: 4)
1177. problem in ligation and cloning - (reply: 1)
1178. Electrophoresis gel boxes and geldoc system - (reply: 2)
1179. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1180. problem with 293T cell co-transfection - (reply: 5)
1181. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1182. how CIP treatment is done - (reply: 3)
1183. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1184. Storage of gel extracted DNA before ligation - (reply: 3)
1185. Dual Luciferase Assay - (reply: 3)
1186. Ligation Problems - (reply: 2)
1187. NICKED DNA - (reply: 2)
1188. cDNA clone - (reply: 1)
1189. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1190. Chosing the right competent cells - (reply: 1)
1191. Checking PCR insert (into pGEMT vector) - (reply: 2)
1192. problem with pGEMT - (reply: 3)
1193. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1194. Luciferase GL4 siRNA - (reply: 5)
1195. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1196. Culture Grow well but No Plasmid - (reply: 2)
1197. Insertion Mutation in Insert Sequence - (reply: 2)
1198. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1199. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1200. Protein expression with T7 promoter - (reply: 1)