Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1171. CaCl2 for making competent cells - (reply: 3)
1172. best way to transport bacteria at room temperature - (reply: 5)
1173. Suicide vector - (reply: 2)
1174. Extremely small bacterial colonies after transformation - (reply: 10)
1175. tranformation efficiency - (reply: 2)
1176. transfection time - (reply: 2)
1177. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1178. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1179. Problem with SmaI restriction digest - (reply: 6)
1180. blunt-end / 3ŽA overhang cloning - (reply: 1)
1181. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1182. overexpression of stable clones - (reply: 1)
1183. functionality of yeast promoter in E. coli - (reply: 1)
1184. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1185. Can't seem to do a simple cloning - (reply: 2)
1186. Histogram from Nanodrop - (reply: 1)
1187. cDNA library construction - I get only one colony (reply: 2)
1188. Cloning into pIBV5-His (not working) - (reply: 1)
1189. Addition of Restriction sites into PCR primers - (reply: 4)
1190. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1191. Streaky DNA digest - (reply: 3)
1192. TOPO TA cloning problem - (reply: 12)
1193. cloning problem - (reply: 2)
1194. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1195. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1196. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1197. Nco1 digest problem - (reply: 4)
1198. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1199. ligation - (reply: 1)
1200. pre-prepararation of competent cells - (reply: 3)