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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. different copy # multimers with linker ligation? - (reply: 2)
1172. Help ADD 3 bases to DNA Sequences - (reply: 6)
1173. My battle with BamHI - (reply: 35)
1174. serious problem in cloning - (reply: 2)
1175. What are "Ella-cre" mice? - (reply: 2)
1176. help with pCMV-myc vector - (reply: 2)
1177. Elution amount - (reply: 5)
1178. keeping restriction digested fragments at -20? - (reply: 5)
1179. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1180. re-digestion - (reply: 1)
1181. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1182. Cloning using BamH1 and HindIII - (reply: 8)
1183. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1184. "competentcy" of my method - (reply: 5)
1185. Vector frameshift problem - (reply: 3)
1186. Screening colonies after transformation - (reply: 2)
1187. z competent cells - (reply: 2)
1188. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1189. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1190. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1191. plasmid extraction kit wash buffer solution - (reply: 3)
1192. molecular "terminology" - (reply: 1)
1193. NdeI digestion - Share your experience (reply: 3)
1194. Multiple bands after amping insert - (reply: 2)
1195. Good tool for sequence validation? - For Windows (reply: 2)
1196. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1197. Silencing of the first cistron - (reply: 1)
1198. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1199. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1200. Stable Mammalian Expression with Retrovirus - (reply: 2)