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1171. Failed Ligation - (reply: 2)
1172. cloning of GroEL - (reply: 3)
1173. Ligation problem - (reply: 9)
1174. Problems with High Background with Gateway Cloning - (reply: 1)
1175. Mutagenesis troubles - (reply: 2)
1176. ligation/transformation failed - (reply: 7)
1177. Transfecting primary cells with plasmid - (reply: 1)
1178. Extra bands after QIAGEN spin miniprep - (reply: 3)
1179. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1180. The use of hygromycin - (reply: 2)
1181. QUESTION about cloning - (reply: 5)
1182. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1183. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1184. Ligation screening - (reply: 4)
1185. Double digestion and ligation problem - (reply: 1)
1186. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1187. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1188. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1189. CMV promoter - (reply: 2)
1190. insert in the reverse orientation after transformation - (reply: 5)
1191. cDNA as PCR template - (reply: 1)
1192. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1193. T4 ligase buffer - (reply: 4)
1194. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1195. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1196. ligation problems - no colonies at the end!!!!! (reply: 4)
1197. Good stable mammalian promoter - (reply: 1)
1198. Self-ligation from a Double Digest - (reply: 4)
1199. Strange frameshift question - (reply: 2)
1200. Plasmid DNA mini-prep - no good isolation (reply: 5)
1172. cloning of GroEL - (reply: 3)
1173. Ligation problem - (reply: 9)
1174. Problems with High Background with Gateway Cloning - (reply: 1)
1175. Mutagenesis troubles - (reply: 2)
1176. ligation/transformation failed - (reply: 7)
1177. Transfecting primary cells with plasmid - (reply: 1)
1178. Extra bands after QIAGEN spin miniprep - (reply: 3)
1179. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1180. The use of hygromycin - (reply: 2)
1181. QUESTION about cloning - (reply: 5)
1182. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1183. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1184. Ligation screening - (reply: 4)
1185. Double digestion and ligation problem - (reply: 1)
1186. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1187. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1188. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1189. CMV promoter - (reply: 2)
1190. insert in the reverse orientation after transformation - (reply: 5)
1191. cDNA as PCR template - (reply: 1)
1192. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1193. T4 ligase buffer - (reply: 4)
1194. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1195. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1196. ligation problems - no colonies at the end!!!!! (reply: 4)
1197. Good stable mammalian promoter - (reply: 1)
1198. Self-ligation from a Double Digest - (reply: 4)
1199. Strange frameshift question - (reply: 2)
1200. Plasmid DNA mini-prep - no good isolation (reply: 5)