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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. My battle with BamHI - (reply: 34)
1172. serious problem in cloning - (reply: 2)
1173. What are "Ella-cre" mice? - (reply: 2)
1174. help with pCMV-myc vector - (reply: 2)
1175. Elution amount - (reply: 5)
1176. keeping restriction digested fragments at -20? - (reply: 5)
1177. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1178. re-digestion - (reply: 1)
1179. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1180. Cloning using BamH1 and HindIII - (reply: 8)
1181. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1182. "competentcy" of my method - (reply: 5)
1183. Vector frameshift problem - (reply: 3)
1184. Screening colonies after transformation - (reply: 2)
1185. z competent cells - (reply: 2)
1186. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1187. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1188. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1189. plasmid extraction kit wash buffer solution - (reply: 3)
1190. molecular "terminology" - (reply: 1)
1191. NdeI digestion - Share your experience (reply: 3)
1192. Multiple bands after amping insert - (reply: 2)
1193. Good tool for sequence validation? - For Windows (reply: 2)
1194. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1195. Silencing of the first cistron - (reply: 1)
1196. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1197. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1198. Stable Mammalian Expression with Retrovirus - (reply: 2)
1199. Neon Transfection System - (reply: 2)
1200. help - (reply: 5)