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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. Miniprep - (reply: 3)
1172. PCR and then ligation - (reply: 7)
1173. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1174. 3 way ligation help needed - (reply: 10)
1175. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1176. Cloning woes - Running out of ideas! - (reply: 7)
1177. What fields of research other than microbiology work with bacteria and - (reply: 1)
1178. How to inoculate bacteria? - (reply: 10)
1179. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1180. need help in my Fse1 restriction ezyme - (reply: 4)
1181. Insert problems with TA cloning - (reply: 1)
1182. Surface expression by tansfection of CHO - (reply: 3)
1183. please help!!! with transformation - (reply: 10)
1184. sequencing primer: T7 or T7promoter? - (reply: 4)
1185. Unstable clone? - (reply: 1)
1186. Restriction enzymes - (reply: 3)
1187. Problems facing in cloning with pBI121 vector - (reply: 1)
1188. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1189. Is my insert present??? - (reply: 17)
1190. Need help for restriction enzymes - (reply: 5)
1191. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1192. Mini prep - (reply: 4)
1193. adding koazak sequence to primer - (reply: 1)
1194. DNA ligation - (reply: 2)
1195. adding C terminal tag to reverse primer - (reply: 1)
1196. glycerol stocks - (reply: 10)
1197. doubts in sequence submission to NCBI - (reply: 2)
1198. Concentration of DNA for Transformation - (reply: 1)
1199. Restriction digest - (reply: 7)
1200. Help! Double restriction digestion failed.... - (reply: 6)