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1171. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1172. GPCR - (reply: 1)
1173. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1174. finding the suitable RE - first timer (reply: 6)
1175. TOPO TA Problems - TOPO TA Problems (reply: 8)
1176. about antibiotic addition - (reply: 4)
1177. about chloramphenicol and L.lactis - (reply: 1)
1178. ligation - (reply: 5)
1179. Losing insert from pGEM? - (reply: 2)
1180. How to concentrate lenti or retroviral particules - (reply: 3)
1181. wierd clot in LB culture - (reply: 5)
1182. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1183. Not getting enough colony - (reply: 4)
1184. Ligation problem - (reply: 9)
1185. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1186. about flanking sequnce - (reply: 3)
1187. Ligation issues - (reply: 4)
1188. messed up restriction sites - (reply: 3)
1189. annoying PCR cloning problem (season 2) - (reply: 5)
1190. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
1191. size limit of plasmid origins - (reply: 2)
1192. Problems with Stratagene Site Directed Mutagenesis - (reply: 36)
1193. confusion about 5'UTR - confusion about 5'UTR (reply: 1)
1194. qPCR - urgent help (reply: 2)
1195. GFP - (reply: 2)
1196. Creating empty vector by self-ligation - (reply: 31)
1197. ligation probs - (reply: 2)
1198. Insert phosphorylation upon Klenow? - (reply: 5)
1199. confusion with different forms of plasmid - (reply: 3)
1200. an annoying cloning - (reply: 9)
1172. GPCR - (reply: 1)
1173. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1174. finding the suitable RE - first timer (reply: 6)
1175. TOPO TA Problems - TOPO TA Problems (reply: 8)
1176. about antibiotic addition - (reply: 4)
1177. about chloramphenicol and L.lactis - (reply: 1)
1178. ligation - (reply: 5)
1179. Losing insert from pGEM? - (reply: 2)
1180. How to concentrate lenti or retroviral particules - (reply: 3)
1181. wierd clot in LB culture - (reply: 5)
1182. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1183. Not getting enough colony - (reply: 4)
1184. Ligation problem - (reply: 9)
1185. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1186. about flanking sequnce - (reply: 3)
1187. Ligation issues - (reply: 4)
1188. messed up restriction sites - (reply: 3)
1189. annoying PCR cloning problem (season 2) - (reply: 5)
1190. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
1191. size limit of plasmid origins - (reply: 2)
1192. Problems with Stratagene Site Directed Mutagenesis - (reply: 36)
1193. confusion about 5'UTR - confusion about 5'UTR (reply: 1)
1194. qPCR - urgent help (reply: 2)
1195. GFP - (reply: 2)
1196. Creating empty vector by self-ligation - (reply: 31)
1197. ligation probs - (reply: 2)
1198. Insert phosphorylation upon Klenow? - (reply: 5)
1199. confusion with different forms of plasmid - (reply: 3)
1200. an annoying cloning - (reply: 9)