Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1171. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1172. CMV promoter - (reply: 2)
1173. insert in the reverse orientation after transformation - (reply: 5)
1174. cDNA as PCR template - (reply: 1)
1175. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1176. T4 ligase buffer - (reply: 4)
1177. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1178. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1179. ligation problems - no colonies at the end!!!!! (reply: 4)
1180. Good stable mammalian promoter - (reply: 1)
1181. Self-ligation from a Double Digest - (reply: 4)
1182. Strange frameshift question - (reply: 2)
1183. Plasmid DNA mini-prep - no good isolation (reply: 5)
1184. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1185. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1186. BstUI digestion - (reply: 1)
1187. I have cloning problems with pGEM-T Easy - (reply: 5)
1188. CaCl2 for making competent cells - (reply: 3)
1189. best way to transport bacteria at room temperature - (reply: 5)
1190. Suicide vector - (reply: 2)
1191. Extremely small bacterial colonies after transformation - (reply: 10)
1192. tranformation efficiency - (reply: 2)
1193. transfection time - (reply: 2)
1194. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1195. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1196. Problem with SmaI restriction digest - (reply: 6)
1197. blunt-end / 3ŽA overhang cloning - (reply: 1)
1198. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1199. overexpression of stable clones - (reply: 1)
1200. functionality of yeast promoter in E. coli - (reply: 1)