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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. Neon Transfection System - (reply: 2)
1172. help - (reply: 5)
1173. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1174. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1175. problems with qiagen gel extraction kit - (reply: 7)
1176. restriction enzyme flanking sequence preferance - (reply: 2)
1177. pCDH Lentivector has 500 bp insert - (reply: 5)
1178. 5' RACE for very GC-rich gene - (reply: 2)
1179. Phosphorylated primers - (reply: 5)
1180. Correct orientation of the insert - (reply: 7)
1181. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1182. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1183. Cloning advice needed - (reply: 4)
1184. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1185. Colony Screeing without using PCR - (reply: 8)
1186. blue white screening - (reply: 3)
1187. phosphorylate dprimers for pfx do not work - (reply: 3)
1188. help - (reply: 1)
1189. problem with yeast transformation - (reply: 2)
1190. ta cloning of pfu amplified fragment - (reply: 9)
1191. how to express the gene in opposite transcriptional direction - (reply: 3)
1192. Water for electrocompetent cells - (reply: 2)
1193. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1194. Bacterial growth in agar plate with ampicillin - (reply: 4)
1195. problem in ligation and cloning - (reply: 1)
1196. Electrophoresis gel boxes and geldoc system - (reply: 2)
1197. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1198. problem with 293T cell co-transfection - (reply: 5)
1199. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1200. how CIP treatment is done - (reply: 3)