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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1172. how CIP treatment is done - (reply: 3)
1173. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1174. Storage of gel extracted DNA before ligation - (reply: 3)
1175. Dual Luciferase Assay - (reply: 3)
1176. Ligation Problems - (reply: 2)
1177. NICKED DNA - (reply: 2)
1178. cDNA clone - (reply: 1)
1179. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1180. Chosing the right competent cells - (reply: 1)
1181. Checking PCR insert (into pGEMT vector) - (reply: 2)
1182. problem with pGEMT - (reply: 3)
1183. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1184. Luciferase GL4 siRNA - (reply: 5)
1185. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1186. Culture Grow well but No Plasmid - (reply: 2)
1187. Insertion Mutation in Insert Sequence - (reply: 2)
1188. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1189. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1190. Protein expression with T7 promoter - (reply: 1)
1191. Triple ligation not working - (reply: 1)
1192. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1193. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1194. Problem with Induction (inclusion bodies?) - (reply: 4)
1195. Colony PCR screen positive - insert digestion negative - (reply: 2)
1196. plasmid purification kit for transfection - (reply: 2)
1197. Which pGL4 vector to choose - (reply: 2)
1198. Qiagen midi prepare low yield - (reply: 6)
1199. Help: incompatible RE site subcloning - (reply: 1)
1200. need help cloning cdna of a known protein - (reply: 1)