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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. Screening colonies after transformation - (reply: 2)
1172. z competent cells - (reply: 2)
1173. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1174. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1175. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1176. plasmid extraction kit wash buffer solution - (reply: 3)
1177. molecular "terminology" - (reply: 1)
1178. NdeI digestion - Share your experience (reply: 3)
1179. Multiple bands after amping insert - (reply: 2)
1180. Good tool for sequence validation? - For Windows (reply: 2)
1181. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1182. Silencing of the first cistron - (reply: 1)
1183. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1184. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1185. Stable Mammalian Expression with Retrovirus - (reply: 2)
1186. Neon Transfection System - (reply: 2)
1187. help - (reply: 5)
1188. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1189. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1190. problems with qiagen gel extraction kit - (reply: 7)
1191. restriction enzyme flanking sequence preferance - (reply: 2)
1192. pCDH Lentivector has 500 bp insert - (reply: 5)
1193. 5' RACE for very GC-rich gene - (reply: 2)
1194. Phosphorylated primers - (reply: 5)
1195. Correct orientation of the insert - (reply: 7)
1196. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1197. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1198. Cloning advice needed - (reply: 4)
1199. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1200. Colony Screeing without using PCR - (reply: 8)