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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1172. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1173. BstUI digestion - (reply: 1)
1174. I have cloning problems with pGEM-T Easy - (reply: 5)
1175. CaCl2 for making competent cells - (reply: 3)
1176. best way to transport bacteria at room temperature - (reply: 5)
1177. Suicide vector - (reply: 2)
1178. Extremely small bacterial colonies after transformation - (reply: 10)
1179. tranformation efficiency - (reply: 2)
1180. transfection time - (reply: 2)
1181. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1182. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1183. Problem with SmaI restriction digest - (reply: 6)
1184. blunt-end / 3ŽA overhang cloning - (reply: 1)
1185. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1186. overexpression of stable clones - (reply: 1)
1187. functionality of yeast promoter in E. coli - (reply: 1)
1188. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1189. Can't seem to do a simple cloning - (reply: 2)
1190. Histogram from Nanodrop - (reply: 1)
1191. cDNA library construction - I get only one colony (reply: 2)
1192. Cloning into pIBV5-His (not working) - (reply: 1)
1193. Addition of Restriction sites into PCR primers - (reply: 4)
1194. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1195. Streaky DNA digest - (reply: 3)
1196. TOPO TA cloning problem - (reply: 12)
1197. cloning problem - (reply: 2)
1198. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1199. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1200. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)