Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1171. Ligation screening - (reply: 4)
1172. Double digestion and ligation problem - (reply: 1)
1173. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1174. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1175. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1176. CMV promoter - (reply: 2)
1177. insert in the reverse orientation after transformation - (reply: 5)
1178. cDNA as PCR template - (reply: 1)
1179. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1180. T4 ligase buffer - (reply: 4)
1181. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1182. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1183. ligation problems - no colonies at the end!!!!! (reply: 4)
1184. Good stable mammalian promoter - (reply: 1)
1185. Self-ligation from a Double Digest - (reply: 4)
1186. Strange frameshift question - (reply: 2)
1187. Plasmid DNA mini-prep - no good isolation (reply: 5)
1188. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1189. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1190. BstUI digestion - (reply: 1)
1191. I have cloning problems with pGEM-T Easy - (reply: 5)
1192. CaCl2 for making competent cells - (reply: 3)
1193. best way to transport bacteria at room temperature - (reply: 5)
1194. Suicide vector - (reply: 2)
1195. Extremely small bacterial colonies after transformation - (reply: 10)
1196. tranformation efficiency - (reply: 2)
1197. transfection time - (reply: 2)
1198. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1199. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1200. Problem with SmaI restriction digest - (reply: 6)