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Top : New Forum Archives (2009-): : Molecular-Cloning
1171. Good stable mammalian promoter - (reply: 1)
1172. Self-ligation from a Double Digest - (reply: 4)
1173. Strange frameshift question - (reply: 2)
1174. Plasmid DNA mini-prep - no good isolation (reply: 5)
1175. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1176. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1177. BstUI digestion - (reply: 1)
1178. I have cloning problems with pGEM-T Easy - (reply: 5)
1179. CaCl2 for making competent cells - (reply: 3)
1180. best way to transport bacteria at room temperature - (reply: 5)
1181. Suicide vector - (reply: 2)
1182. Extremely small bacterial colonies after transformation - (reply: 10)
1183. tranformation efficiency - (reply: 2)
1184. transfection time - (reply: 2)
1185. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1186. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1187. Problem with SmaI restriction digest - (reply: 6)
1188. blunt-end / 3ŽA overhang cloning - (reply: 1)
1189. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1190. overexpression of stable clones - (reply: 1)
1191. functionality of yeast promoter in E. coli - (reply: 1)
1192. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1193. Can't seem to do a simple cloning - (reply: 2)
1194. Histogram from Nanodrop - (reply: 1)
1195. cDNA library construction - I get only one colony (reply: 2)
1196. Cloning into pIBV5-His (not working) - (reply: 1)
1197. Addition of Restriction sites into PCR primers - (reply: 4)
1198. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1199. Streaky DNA digest - (reply: 3)
1200. TOPO TA cloning problem - (reply: 12)