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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
662. XhoI digestion - (reply: 12)
663. Lenti vector ligation - (reply: 2)
664. Cloning from a pGEMT Easy vector - (reply: 3)
665. Distance between RE sites for double digestion - (reply: 5)
666. Plasmid compatible with pBAD24 - (reply: 1)
667. I cant get colony - (reply: 3)
668. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
669. Vector with inducible/repressible system - (reply: 1)
670. cloning problem - (reply: 9)
671. Cloning genes into plant vector - (reply: 1)
672. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
673. Three-way ligation in two steps? - (reply: 3)
674. How does circular, uncut pGEM-T migrate? - (reply: 2)
675. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
676. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
677. Cloning App Released from Life Technologies - (reply: 2)
678. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
679. Dissolve IPTG in EtOH - (reply: 3)
680. problem with transient transfection - (reply: 6)
681. Recombinant construct looks smaller than empty vector - (reply: 3)
682. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
683. hygromycin concentration for e-coli selection - (reply: 1)
684. orientation of promoter into viral construct - (reply: 1)
685. problems with PCR confirmation of insert . HELP ! - (reply: 1)
686. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
687. Loss of promoter during ligation - (reply: 2)
688. Enzyme restriction - (reply: 8)
689. Colony PCR Problem!!!! - (reply: 5)
690. Removing unwanted sequence from pUC19 - (reply: 4)