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Top : New Forum Archives (2009-): : Molecular-Cloning
661. E.coli grow on neg. control - (reply: 1)
662. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
663. Need help troubleshooting digest. - (reply: 1)
664. Competent cells question: JM101 vs DH5alpha - (reply: 2)
665. How to avoid plasmid instability - (reply: 4)
666. Question Help - (reply: 1)
667. Shear-induced damage of plasmid by vortexing - (reply: 1)
668. Problems with blunt end ligation - (reply: 3)
669. Problem with Transforming E. coli DH10Bac - (reply: 8)
670. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
671. Heat inactivate ligase - (reply: 5)
672. DMSO stock of overnight bacterial cultures - (reply: 1)
673. Recommended competent cell for transformation of large plasmids? - (reply: 4)
674. Prolem in screening of hygromycin concentration - (reply: 1)
675. Primers for Introduction of new restriction sites to a vector - (reply: 3)
676. problem in cloning involving partial digestion - (reply: 1)
677. Why perform restriction enzyme digestion again? - (reply: 1)
678. Cotransient Transfections DNA Requirements - (reply: 3)
679. which software to draw the scheme of cloning strategy - (reply: 5)
680. Help with restriction analysis - (reply: 1)
681. Sequencing of PCR product - (reply: 4)
682. KanR sequence - (reply: 2)
683. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
684. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
685. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
686. Plasmid digestion after transformation - (reply: 4)
687. Blunt end ligation insert:vector ratio - (reply: 1)
688. PCRed on restriction sites -- how do I purify it? - (reply: 8)
689. Puzzle about plamid and its digest, plz help - (reply: 8)
690. Smear above plasmid dna - (reply: 3)