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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Problems with blunt end ligation - (reply: 3)
662. Problem with Transforming E. coli DH10Bac - (reply: 8)
663. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
664. Heat inactivate ligase - (reply: 5)
665. DMSO stock of overnight bacterial cultures - (reply: 1)
666. Recommended competent cell for transformation of large plasmids? - (reply: 4)
667. Prolem in screening of hygromycin concentration - (reply: 1)
668. Primers for Introduction of new restriction sites to a vector - (reply: 3)
669. problem in cloning involving partial digestion - (reply: 1)
670. Why perform restriction enzyme digestion again? - (reply: 1)
671. Cotransient Transfections DNA Requirements - (reply: 3)
672. which software to draw the scheme of cloning strategy - (reply: 5)
673. Help with restriction analysis - (reply: 1)
674. Sequencing of PCR product - (reply: 4)
675. KanR sequence - (reply: 2)
676. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
677. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
678. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
679. Plasmid digestion after transformation - (reply: 4)
680. Blunt end ligation insert:vector ratio - (reply: 1)
681. PCRed on restriction sites -- how do I purify it? - (reply: 8)
682. Puzzle about plamid and its digest, plz help - (reply: 8)
683. Smear above plasmid dna - (reply: 3)
684. restriction digestion buffers contaminated with dnase - (reply: 3)
685. Bacterial Induction - (reply: 1)
686. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
687. smear restriction digest - (reply: 1)
688. How to set the Sonicator to 20%amplitude? - (reply: 3)
689. No DNA after midiprep - (reply: 2)
690. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)