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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Plasmid digestion after transformation - (reply: 4)
662. Blunt end ligation insert:vector ratio - (reply: 1)
663. PCRed on restriction sites -- how do I purify it? - (reply: 8)
664. Puzzle about plamid and its digest, plz help - (reply: 8)
665. Smear above plasmid dna - (reply: 3)
666. restriction digestion buffers contaminated with dnase - (reply: 3)
667. Bacterial Induction - (reply: 1)
668. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
669. smear restriction digest - (reply: 1)
670. How to set the Sonicator to 20%amplitude? - (reply: 3)
671. No DNA after midiprep - (reply: 2)
672. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
673. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
674. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
675. Impact of primer sequence on TA cloning - (reply: 3)
676. Maximum length of cDNA from RT reaction - (reply: 6)
677. overlapping PCR protocol - (reply: 12)
678. cloning an AT rich gene - (reply: 3)
679. Cloning two genes in the same plasmid - (reply: 4)
680. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
681. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
682. Problem: Cloning DNA shuffled band - (reply: 2)
683. shRNA to primary keratinocytes - (reply: 1)
684. Enzymetic digestion with ApaI and HincII - (reply: 2)
685. Ligation gel picture interpretation - (reply: 4)
686. strange subcloning problem - (reply: 9)
687. Cloned sequence is not predicted size by western blot - (reply: 1)
688. Ligation of insert to itself problem - (reply: 3)
689. double digest problem - (reply: 3)
690. Difficulty in expressing fusion protein - (reply: 1)