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Top : New Forum Archives (2009-): : Molecular-Cloning
661. How to avoid plasmid instability - (reply: 4)
662. Question Help - (reply: 1)
663. Shear-induced damage of plasmid by vortexing - (reply: 1)
664. Problems with blunt end ligation - (reply: 3)
665. Problem with Transforming E. coli DH10Bac - (reply: 8)
666. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
667. Heat inactivate ligase - (reply: 5)
668. DMSO stock of overnight bacterial cultures - (reply: 1)
669. Recommended competent cell for transformation of large plasmids? - (reply: 4)
670. Prolem in screening of hygromycin concentration - (reply: 1)
671. Primers for Introduction of new restriction sites to a vector - (reply: 3)
672. problem in cloning involving partial digestion - (reply: 1)
673. Why perform restriction enzyme digestion again? - (reply: 1)
674. Cotransient Transfections DNA Requirements - (reply: 3)
675. which software to draw the scheme of cloning strategy - (reply: 5)
676. Help with restriction analysis - (reply: 1)
677. Sequencing of PCR product - (reply: 4)
678. KanR sequence - (reply: 2)
679. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
680. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
681. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
682. Plasmid digestion after transformation - (reply: 4)
683. Blunt end ligation insert:vector ratio - (reply: 1)
684. PCRed on restriction sites -- how do I purify it? - (reply: 8)
685. Puzzle about plamid and its digest, plz help - (reply: 8)
686. Smear above plasmid dna - (reply: 3)
687. restriction digestion buffers contaminated with dnase - (reply: 3)
688. Bacterial Induction - (reply: 1)
689. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
690. smear restriction digest - (reply: 1)