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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Help with restriction analysis - (reply: 1)
662. Sequencing of PCR product - (reply: 4)
663. KanR sequence - (reply: 2)
664. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
665. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
666. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
667. Plasmid digestion after transformation - (reply: 4)
668. Blunt end ligation insert:vector ratio - (reply: 1)
669. PCRed on restriction sites -- how do I purify it? - (reply: 8)
670. Puzzle about plamid and its digest, plz help - (reply: 8)
671. Smear above plasmid dna - (reply: 3)
672. restriction digestion buffers contaminated with dnase - (reply: 3)
673. Bacterial Induction - (reply: 1)
674. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
675. smear restriction digest - (reply: 1)
676. How to set the Sonicator to 20%amplitude? - (reply: 3)
677. No DNA after midiprep - (reply: 2)
678. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
679. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
680. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
681. Impact of primer sequence on TA cloning - (reply: 3)
682. Maximum length of cDNA from RT reaction - (reply: 6)
683. overlapping PCR protocol - (reply: 12)
684. cloning an AT rich gene - (reply: 3)
685. Cloning two genes in the same plasmid - (reply: 4)
686. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
687. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
688. Problem: Cloning DNA shuffled band - (reply: 2)
689. shRNA to primary keratinocytes - (reply: 1)
690. Enzymetic digestion with ApaI and HincII - (reply: 2)