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Top : New Forum Archives (2009-): : Molecular-Cloning
661. PCRed on restriction sites -- how do I purify it? - (reply: 8)
662. Puzzle about plamid and its digest, plz help - (reply: 8)
663. Smear above plasmid dna - (reply: 3)
664. restriction digestion buffers contaminated with dnase - (reply: 3)
665. Bacterial Induction - (reply: 1)
666. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
667. smear restriction digest - (reply: 1)
668. How to set the Sonicator to 20%amplitude? - (reply: 3)
669. No DNA after midiprep - (reply: 2)
670. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
671. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
672. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
673. Impact of primer sequence on TA cloning - (reply: 3)
674. Maximum length of cDNA from RT reaction - (reply: 6)
675. overlapping PCR protocol - (reply: 12)
676. cloning an AT rich gene - (reply: 3)
677. Cloning two genes in the same plasmid - (reply: 4)
678. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
679. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
680. Problem: Cloning DNA shuffled band - (reply: 2)
681. shRNA to primary keratinocytes - (reply: 1)
682. Enzymetic digestion with ApaI and HincII - (reply: 2)
683. Ligation gel picture interpretation - (reply: 4)
684. strange subcloning problem - (reply: 9)
685. Cloned sequence is not predicted size by western blot - (reply: 1)
686. Ligation of insert to itself problem - (reply: 3)
687. double digest problem - (reply: 3)
688. Difficulty in expressing fusion protein - (reply: 1)
689. Plasmid recovery problem - (reply: 3)
690. Restriction Digest Problem - (reply: 5)