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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Maximum length of cDNA from RT reaction - (reply: 6)
662. overlapping PCR protocol - (reply: 12)
663. cloning an AT rich gene - (reply: 3)
664. Cloning two genes in the same plasmid - (reply: 4)
665. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
666. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
667. Problem: Cloning DNA shuffled band - (reply: 2)
668. shRNA to primary keratinocytes - (reply: 1)
669. Enzymetic digestion with ApaI and HincII - (reply: 2)
670. Ligation gel picture interpretation - (reply: 4)
671. strange subcloning problem - (reply: 9)
672. Cloned sequence is not predicted size by western blot - (reply: 1)
673. Ligation of insert to itself problem - (reply: 3)
674. double digest problem - (reply: 3)
675. Difficulty in expressing fusion protein - (reply: 1)
676. Plasmid recovery problem - (reply: 3)
677. Restriction Digest Problem - (reply: 5)
678. Recombination protocol needed - (reply: 2)
679. Cloning problem using pET32a vector - (reply: 3)
680. Fixing SNPs after cloning - (reply: 5)
681. Electroporated plasmid loses the insert - (reply: 8)
682. Recombination due to overselective plate? - (reply: 11)
683. Help with ligation - (reply: 2)
684. emty vector cloning - (reply: 3)
685. RE site on complimentary strand of plasmid? - (reply: 1)
686. LENTIVIRUS transfection efficiency - (reply: 1)
687. Double digest after cloning doesn't give gene - (reply: 6)
688. Blunt end PCR product ligation - (reply: 1)
689. Screening for clones - (reply: 2)
690. Plasmid isolation Qiagen - (reply: 2)