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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Cotransient Transfections DNA Requirements - (reply: 3)
662. which software to draw the scheme of cloning strategy - (reply: 5)
663. Help with restriction analysis - (reply: 1)
664. Sequencing of PCR product - (reply: 4)
665. KanR sequence - (reply: 2)
666. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
667. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
668. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
669. Plasmid digestion after transformation - (reply: 4)
670. Blunt end ligation insert:vector ratio - (reply: 1)
671. PCRed on restriction sites -- how do I purify it? - (reply: 8)
672. Puzzle about plamid and its digest, plz help - (reply: 8)
673. Smear above plasmid dna - (reply: 3)
674. restriction digestion buffers contaminated with dnase - (reply: 3)
675. Bacterial Induction - (reply: 1)
676. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
677. smear restriction digest - (reply: 1)
678. How to set the Sonicator to 20%amplitude? - (reply: 3)
679. No DNA after midiprep - (reply: 2)
680. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
681. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
682. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
683. Impact of primer sequence on TA cloning - (reply: 3)
684. Maximum length of cDNA from RT reaction - (reply: 6)
685. overlapping PCR protocol - (reply: 12)
686. cloning an AT rich gene - (reply: 3)
687. Cloning two genes in the same plasmid - (reply: 4)
688. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
689. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
690. Problem: Cloning DNA shuffled band - (reply: 2)