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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
662. need help with subcloning !! - (reply: 2)
663. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
664. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
665. cdna library. - (reply: 2)
666. Help in RE digestion of vector - (reply: 5)
667. bacterial transformation problem - (reply: 2)
668. Transformation - (reply: 3)
669. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
670. What does vortexing do in transformation? - (reply: 1)
671. failure in miniprep - (reply: 1)
672. Host cells for transformation - (reply: 3)
673. making recombinant protein - (reply: 2)
674. Confirming insert???? - (reply: 1)
675. how to make a recombinant protein with signal peptide - (reply: 1)
676. How to add restriction enzyme site into the DNA fragment - (reply: 3)
677. Issues with ligation - (reply: 2)
678. Ligation of double insert into pET31 failing - (reply: 1)
679. Homemade MiniPreps for Plasmid Purification - (reply: 1)
680. Agar has dried out during cultivation - (reply: 1)
681. Gateway cloning vector compatibility - (reply: 2)
682. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
683. Cloning of IL8-promoter - (reply: 3)
684. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
685. Template DNA (plasmid) concentration for PCR - (reply: 4)
686. Ligation of 16kb PCR product - (reply: 5)
687. stable transfection - (reply: 1)
688. How to choose the right plasmids for certain types of cell? - (reply: 1)
689. Ligation needed after SDM with PfuX? - (reply: 3)
690. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)