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Top : New Forum Archives (2009-): : Molecular-Cloning
661. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
662. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
663. In-Fusion (Clontech) Reaction buffer - (reply: 1)
664. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
665. MDA-MB-231 cell transfection - (reply: 4)
666. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
667. Break Cell Clumps - prepare single cells for injections (reply: 1)
668. 126pb insert clonnig/ digestion issues - (reply: 3)
669. cloning problem... - (reply: 5)
670. yeast glycerol stock - (reply: 2)
671. yeast transformation - (reply: 1)
672. Problems with ligation and transformation - (reply: 2)
673. very small insert and a very ling vector - (reply: 1)
674. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
675. cDNA clone - (reply: 1)
676. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
677. why these guys use T7 instead of CMV? - for transfection (reply: 2)
678. Electroporation - no colonies (reply: 1)
679. enzymes inactivation - (reply: 3)
680. help: why plasmid yield dropped ever since the first culture - (reply: 2)
681. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
682. miRNA cloning - (reply: 2)
683. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
684. Single-digested vector shifts downward on gel - (reply: 1)
685. problem in digestion - (reply: 1)
686. Cloning does not work - (reply: 4)
687. Small Colonies After Transformation - (reply: 5)
688. Ligation and transformation problem - (reply: 2)
689. Agrobacterium EHA101 competent cells - (reply: 1)
690. restriction digestion against colony PCR - (reply: 1)