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Top : New Forum Archives (2009-): : Molecular-Cloning
661. KanR sequence - (reply: 2)
662. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
663. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
664. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
665. Plasmid digestion after transformation - (reply: 4)
666. Blunt end ligation insert:vector ratio - (reply: 1)
667. PCRed on restriction sites -- how do I purify it? - (reply: 8)
668. Puzzle about plamid and its digest, plz help - (reply: 8)
669. Smear above plasmid dna - (reply: 3)
670. restriction digestion buffers contaminated with dnase - (reply: 3)
671. Bacterial Induction - (reply: 1)
672. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
673. smear restriction digest - (reply: 1)
674. How to set the Sonicator to 20%amplitude? - (reply: 3)
675. No DNA after midiprep - (reply: 2)
676. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
677. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
678. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
679. Impact of primer sequence on TA cloning - (reply: 3)
680. Maximum length of cDNA from RT reaction - (reply: 6)
681. overlapping PCR protocol - (reply: 12)
682. cloning an AT rich gene - (reply: 3)
683. Cloning two genes in the same plasmid - (reply: 4)
684. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
685. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
686. Problem: Cloning DNA shuffled band - (reply: 2)
687. shRNA to primary keratinocytes - (reply: 1)
688. Enzymetic digestion with ApaI and HincII - (reply: 2)
689. Ligation gel picture interpretation - (reply: 4)
690. strange subcloning problem - (reply: 9)