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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Need help troubleshooting digest. - (reply: 1)
662. Competent cells question: JM101 vs DH5alpha - (reply: 2)
663. How to avoid plasmid instability - (reply: 4)
664. Question Help - (reply: 1)
665. Shear-induced damage of plasmid by vortexing - (reply: 1)
666. Problems with blunt end ligation - (reply: 3)
667. Problem with Transforming E. coli DH10Bac - (reply: 8)
668. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
669. Heat inactivate ligase - (reply: 5)
670. DMSO stock of overnight bacterial cultures - (reply: 1)
671. Recommended competent cell for transformation of large plasmids? - (reply: 4)
672. Prolem in screening of hygromycin concentration - (reply: 1)
673. Primers for Introduction of new restriction sites to a vector - (reply: 3)
674. problem in cloning involving partial digestion - (reply: 1)
675. Why perform restriction enzyme digestion again? - (reply: 1)
676. Cotransient Transfections DNA Requirements - (reply: 3)
677. which software to draw the scheme of cloning strategy - (reply: 5)
678. Help with restriction analysis - (reply: 1)
679. Sequencing of PCR product - (reply: 4)
680. KanR sequence - (reply: 2)
681. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
682. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
683. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
684. Plasmid digestion after transformation - (reply: 4)
685. Blunt end ligation insert:vector ratio - (reply: 1)
686. PCRed on restriction sites -- how do I purify it? - (reply: 8)
687. Puzzle about plamid and its digest, plz help - (reply: 8)
688. Smear above plasmid dna - (reply: 3)
689. restriction digestion buffers contaminated with dnase - (reply: 3)
690. Bacterial Induction - (reply: 1)