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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Why perform restriction enzyme digestion again? - (reply: 1)
662. Cotransient Transfections DNA Requirements - (reply: 3)
663. which software to draw the scheme of cloning strategy - (reply: 5)
664. Help with restriction analysis - (reply: 1)
665. Sequencing of PCR product - (reply: 4)
666. KanR sequence - (reply: 2)
667. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
668. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
669. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
670. Plasmid digestion after transformation - (reply: 4)
671. Blunt end ligation insert:vector ratio - (reply: 1)
672. PCRed on restriction sites -- how do I purify it? - (reply: 8)
673. Puzzle about plamid and its digest, plz help - (reply: 8)
674. Smear above plasmid dna - (reply: 3)
675. restriction digestion buffers contaminated with dnase - (reply: 3)
676. Bacterial Induction - (reply: 1)
677. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
678. smear restriction digest - (reply: 1)
679. How to set the Sonicator to 20%amplitude? - (reply: 3)
680. No DNA after midiprep - (reply: 2)
681. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
682. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
683. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
684. Impact of primer sequence on TA cloning - (reply: 3)
685. Maximum length of cDNA from RT reaction - (reply: 6)
686. overlapping PCR protocol - (reply: 12)
687. cloning an AT rich gene - (reply: 3)
688. Cloning two genes in the same plasmid - (reply: 4)
689. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
690. why my solution II turns cloudy during plasmid extraction? - (reply: 6)