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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Blunt end ligation insert:vector ratio - (reply: 1)
662. PCRed on restriction sites -- how do I purify it? - (reply: 8)
663. Puzzle about plamid and its digest, plz help - (reply: 8)
664. Smear above plasmid dna - (reply: 3)
665. restriction digestion buffers contaminated with dnase - (reply: 3)
666. Bacterial Induction - (reply: 1)
667. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
668. smear restriction digest - (reply: 1)
669. How to set the Sonicator to 20%amplitude? - (reply: 3)
670. No DNA after midiprep - (reply: 2)
671. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
672. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
673. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
674. Impact of primer sequence on TA cloning - (reply: 3)
675. Maximum length of cDNA from RT reaction - (reply: 6)
676. overlapping PCR protocol - (reply: 12)
677. cloning an AT rich gene - (reply: 3)
678. Cloning two genes in the same plasmid - (reply: 4)
679. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
680. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
681. Problem: Cloning DNA shuffled band - (reply: 2)
682. shRNA to primary keratinocytes - (reply: 1)
683. Enzymetic digestion with ApaI and HincII - (reply: 2)
684. Ligation gel picture interpretation - (reply: 4)
685. strange subcloning problem - (reply: 9)
686. Cloned sequence is not predicted size by western blot - (reply: 1)
687. Ligation of insert to itself problem - (reply: 3)
688. double digest problem - (reply: 3)
689. Difficulty in expressing fusion protein - (reply: 1)
690. Plasmid recovery problem - (reply: 3)