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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Question Help - (reply: 1)
662. Shear-induced damage of plasmid by vortexing - (reply: 1)
663. Problems with blunt end ligation - (reply: 3)
664. Problem with Transforming E. coli DH10Bac - (reply: 8)
665. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
666. Heat inactivate ligase - (reply: 5)
667. DMSO stock of overnight bacterial cultures - (reply: 1)
668. Recommended competent cell for transformation of large plasmids? - (reply: 4)
669. Prolem in screening of hygromycin concentration - (reply: 1)
670. Primers for Introduction of new restriction sites to a vector - (reply: 3)
671. problem in cloning involving partial digestion - (reply: 1)
672. Why perform restriction enzyme digestion again? - (reply: 1)
673. Cotransient Transfections DNA Requirements - (reply: 3)
674. which software to draw the scheme of cloning strategy - (reply: 5)
675. Help with restriction analysis - (reply: 1)
676. Sequencing of PCR product - (reply: 4)
677. KanR sequence - (reply: 2)
678. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
679. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
680. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
681. Plasmid digestion after transformation - (reply: 4)
682. Blunt end ligation insert:vector ratio - (reply: 1)
683. PCRed on restriction sites -- how do I purify it? - (reply: 8)
684. Puzzle about plamid and its digest, plz help - (reply: 8)
685. Smear above plasmid dna - (reply: 3)
686. restriction digestion buffers contaminated with dnase - (reply: 3)
687. Bacterial Induction - (reply: 1)
688. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
689. smear restriction digest - (reply: 1)
690. How to set the Sonicator to 20%amplitude? - (reply: 3)