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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Screening for clones - (reply: 2)
662. Plasmid isolation Qiagen - (reply: 2)
663. Restriction Site Destruction - (reply: 1)
664. The copy number of pPICZ alpha A - (reply: 6)
665. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
666. need help with subcloning !! - (reply: 2)
667. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
668. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
669. cdna library. - (reply: 2)
670. Help in RE digestion of vector - (reply: 5)
671. bacterial transformation problem - (reply: 2)
672. Transformation - (reply: 3)
673. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
674. What does vortexing do in transformation? - (reply: 1)
675. failure in miniprep - (reply: 1)
676. Host cells for transformation - (reply: 3)
677. making recombinant protein - (reply: 2)
678. Confirming insert???? - (reply: 1)
679. how to make a recombinant protein with signal peptide - (reply: 1)
680. How to add restriction enzyme site into the DNA fragment - (reply: 3)
681. Issues with ligation - (reply: 2)
682. Ligation of double insert into pET31 failing - (reply: 1)
683. Homemade MiniPreps for Plasmid Purification - (reply: 1)
684. Agar has dried out during cultivation - (reply: 1)
685. Gateway cloning vector compatibility - (reply: 2)
686. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
687. Cloning of IL8-promoter - (reply: 3)
688. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
689. Template DNA (plasmid) concentration for PCR - (reply: 4)
690. Ligation of 16kb PCR product - (reply: 5)