Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
661. Cloning of IL8-promoter - (reply: 3)
662. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
663. Template DNA (plasmid) concentration for PCR - (reply: 4)
664. Ligation of 16kb PCR product - (reply: 5)
665. stable transfection - (reply: 1)
666. How to choose the right plasmids for certain types of cell? - (reply: 1)
667. Ligation needed after SDM with PfuX? - (reply: 3)
668. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
669. Cloning promoter (overlap with another gene!) - (reply: 3)
670. bacterial transfeomation - (reply: 2)
671. Loss of insert? - (reply: 1)
672. Sequencing trouble - (reply: 7)
673. Platinum-A cell trasnfection toruble - (reply: 1)
674. Cloning a promoter - (reply: 2)
675. pcDNA 3.1 His Expression - (reply: 2)
676. Cloning two fragments into a vector - (reply: 3)
677. confirmation of insert in to vector - (reply: 3)
678. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
679. Question about Molar ratio - (reply: 4)
680. Ligation problem - (reply: 16)
681. double digestion with sac1, hindIII - (reply: 3)
682. self-ligation Anneled oligos - (reply: 7)
683. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
684. Looking for a GFP plasmid (non-amp) - (reply: 3)
685. clonning problem (recombined by-products) - (reply: 8)
686. Genecloning three types of molecules? - (reply: 11)
687. help in digesting DNA fragment using restriction enzyme - (reply: 2)
688. Tansformation fails ,how can i make it ? - (reply: 3)
689. pelB leader - how to use - (reply: 1)
690. Transformation bacteria heat chock and incubation? - (reply: 2)