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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Digestion - (reply: 2)
662. Digestion of Genomic DNA - (reply: 1)
663. Isolation of a Gene of Interest for Subcloning - (reply: 8)
664. E.coli grow on neg. control - (reply: 1)
665. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
666. Need help troubleshooting digest. - (reply: 1)
667. Competent cells question: JM101 vs DH5alpha - (reply: 2)
668. How to avoid plasmid instability - (reply: 4)
669. Question Help - (reply: 1)
670. Shear-induced damage of plasmid by vortexing - (reply: 1)
671. Problems with blunt end ligation - (reply: 3)
672. Problem with Transforming E. coli DH10Bac - (reply: 8)
673. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
674. Heat inactivate ligase - (reply: 5)
675. DMSO stock of overnight bacterial cultures - (reply: 1)
676. Recommended competent cell for transformation of large plasmids? - (reply: 4)
677. Prolem in screening of hygromycin concentration - (reply: 1)
678. Primers for Introduction of new restriction sites to a vector - (reply: 3)
679. problem in cloning involving partial digestion - (reply: 1)
680. Why perform restriction enzyme digestion again? - (reply: 1)
681. Cotransient Transfections DNA Requirements - (reply: 3)
682. which software to draw the scheme of cloning strategy - (reply: 5)
683. Help with restriction analysis - (reply: 1)
684. Sequencing of PCR product - (reply: 4)
685. KanR sequence - (reply: 2)
686. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
687. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
688. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
689. Plasmid digestion after transformation - (reply: 4)
690. Blunt end ligation insert:vector ratio - (reply: 1)