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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Problem with Transforming E. coli DH10Bac - (reply: 8)
662. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
663. Heat inactivate ligase - (reply: 5)
664. DMSO stock of overnight bacterial cultures - (reply: 1)
665. Recommended competent cell for transformation of large plasmids? - (reply: 4)
666. Prolem in screening of hygromycin concentration - (reply: 1)
667. Primers for Introduction of new restriction sites to a vector - (reply: 3)
668. problem in cloning involving partial digestion - (reply: 1)
669. Why perform restriction enzyme digestion again? - (reply: 1)
670. Cotransient Transfections DNA Requirements - (reply: 3)
671. which software to draw the scheme of cloning strategy - (reply: 5)
672. Help with restriction analysis - (reply: 1)
673. Sequencing of PCR product - (reply: 4)
674. KanR sequence - (reply: 2)
675. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
676. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
677. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
678. Plasmid digestion after transformation - (reply: 4)
679. Blunt end ligation insert:vector ratio - (reply: 1)
680. PCRed on restriction sites -- how do I purify it? - (reply: 8)
681. Puzzle about plamid and its digest, plz help - (reply: 8)
682. Smear above plasmid dna - (reply: 3)
683. restriction digestion buffers contaminated with dnase - (reply: 3)
684. Bacterial Induction - (reply: 1)
685. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
686. smear restriction digest - (reply: 1)
687. How to set the Sonicator to 20%amplitude? - (reply: 3)
688. No DNA after midiprep - (reply: 2)
689. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
690. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)