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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Isolation of a Gene of Interest for Subcloning - (reply: 8)
662. E.coli grow on neg. control - (reply: 1)
663. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
664. Need help troubleshooting digest. - (reply: 1)
665. Competent cells question: JM101 vs DH5alpha - (reply: 2)
666. How to avoid plasmid instability - (reply: 4)
667. Question Help - (reply: 1)
668. Shear-induced damage of plasmid by vortexing - (reply: 1)
669. Problems with blunt end ligation - (reply: 3)
670. Problem with Transforming E. coli DH10Bac - (reply: 8)
671. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
672. Heat inactivate ligase - (reply: 5)
673. DMSO stock of overnight bacterial cultures - (reply: 1)
674. Recommended competent cell for transformation of large plasmids? - (reply: 4)
675. Prolem in screening of hygromycin concentration - (reply: 1)
676. Primers for Introduction of new restriction sites to a vector - (reply: 3)
677. problem in cloning involving partial digestion - (reply: 1)
678. Why perform restriction enzyme digestion again? - (reply: 1)
679. Cotransient Transfections DNA Requirements - (reply: 3)
680. which software to draw the scheme of cloning strategy - (reply: 5)
681. Help with restriction analysis - (reply: 1)
682. Sequencing of PCR product - (reply: 4)
683. KanR sequence - (reply: 2)
684. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
685. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
686. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
687. Plasmid digestion after transformation - (reply: 4)
688. Blunt end ligation insert:vector ratio - (reply: 1)
689. PCRed on restriction sites -- how do I purify it? - (reply: 8)
690. Puzzle about plamid and its digest, plz help - (reply: 8)