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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Digestion of Genomic DNA - (reply: 1)
662. Isolation of a Gene of Interest for Subcloning - (reply: 8)
663. E.coli grow on neg. control - (reply: 1)
664. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
665. Need help troubleshooting digest. - (reply: 1)
666. Competent cells question: JM101 vs DH5alpha - (reply: 2)
667. How to avoid plasmid instability - (reply: 4)
668. Question Help - (reply: 1)
669. Shear-induced damage of plasmid by vortexing - (reply: 1)
670. Problems with blunt end ligation - (reply: 3)
671. Problem with Transforming E. coli DH10Bac - (reply: 8)
672. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
673. Heat inactivate ligase - (reply: 5)
674. DMSO stock of overnight bacterial cultures - (reply: 1)
675. Recommended competent cell for transformation of large plasmids? - (reply: 4)
676. Prolem in screening of hygromycin concentration - (reply: 1)
677. Primers for Introduction of new restriction sites to a vector - (reply: 3)
678. problem in cloning involving partial digestion - (reply: 1)
679. Why perform restriction enzyme digestion again? - (reply: 1)
680. Cotransient Transfections DNA Requirements - (reply: 3)
681. which software to draw the scheme of cloning strategy - (reply: 5)
682. Help with restriction analysis - (reply: 1)
683. Sequencing of PCR product - (reply: 4)
684. KanR sequence - (reply: 2)
685. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
686. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
687. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
688. Plasmid digestion after transformation - (reply: 4)
689. Blunt end ligation insert:vector ratio - (reply: 1)
690. PCRed on restriction sites -- how do I purify it? - (reply: 8)