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Top : New Forum Archives (2009-): : Molecular-Cloning
661. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
662. Plasmid digestion after transformation - (reply: 4)
663. Blunt end ligation insert:vector ratio - (reply: 1)
664. PCRed on restriction sites -- how do I purify it? - (reply: 8)
665. Puzzle about plamid and its digest, plz help - (reply: 8)
666. Smear above plasmid dna - (reply: 3)
667. restriction digestion buffers contaminated with dnase - (reply: 3)
668. Bacterial Induction - (reply: 1)
669. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
670. smear restriction digest - (reply: 1)
671. How to set the Sonicator to 20%amplitude? - (reply: 3)
672. No DNA after midiprep - (reply: 2)
673. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
674. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
675. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
676. Impact of primer sequence on TA cloning - (reply: 3)
677. Maximum length of cDNA from RT reaction - (reply: 6)
678. overlapping PCR protocol - (reply: 12)
679. cloning an AT rich gene - (reply: 3)
680. Cloning two genes in the same plasmid - (reply: 4)
681. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
682. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
683. Problem: Cloning DNA shuffled band - (reply: 2)
684. shRNA to primary keratinocytes - (reply: 1)
685. Enzymetic digestion with ApaI and HincII - (reply: 2)
686. Ligation gel picture interpretation - (reply: 4)
687. strange subcloning problem - (reply: 9)
688. Cloned sequence is not predicted size by western blot - (reply: 1)
689. Ligation of insert to itself problem - (reply: 3)
690. double digest problem - (reply: 3)