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Top : New Forum Archives (2009-): : Molecular-Cloning
661. No DNA after midiprep - (reply: 2)
662. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
663. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
664. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
665. Impact of primer sequence on TA cloning - (reply: 3)
666. Maximum length of cDNA from RT reaction - (reply: 6)
667. overlapping PCR protocol - (reply: 12)
668. cloning an AT rich gene - (reply: 3)
669. Cloning two genes in the same plasmid - (reply: 4)
670. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
671. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
672. Problem: Cloning DNA shuffled band - (reply: 2)
673. shRNA to primary keratinocytes - (reply: 1)
674. Enzymetic digestion with ApaI and HincII - (reply: 2)
675. Ligation gel picture interpretation - (reply: 4)
676. strange subcloning problem - (reply: 9)
677. Cloned sequence is not predicted size by western blot - (reply: 1)
678. Ligation of insert to itself problem - (reply: 3)
679. double digest problem - (reply: 3)
680. Difficulty in expressing fusion protein - (reply: 1)
681. Plasmid recovery problem - (reply: 3)
682. Restriction Digest Problem - (reply: 5)
683. Recombination protocol needed - (reply: 2)
684. Cloning problem using pET32a vector - (reply: 3)
685. Fixing SNPs after cloning - (reply: 5)
686. Electroporated plasmid loses the insert - (reply: 8)
687. Recombination due to overselective plate? - (reply: 11)
688. Help with ligation - (reply: 2)
689. emty vector cloning - (reply: 3)
690. RE site on complimentary strand of plasmid? - (reply: 1)