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Top : New Forum Archives (2009-): : Molecular-Cloning
1441. Promoter/gene cloning question - (reply: 7)
1442. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1443. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1444. vivo expression technology - ivet (reply: 25)
1445. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
1446. insert is missing after TA cloning - TA cloning (reply: 6)
1447. glycerol stock - shelf life (reply: 5)
1448. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1449. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1450. cloning problem - molecular cloning (reply: 1)
1451. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1452. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1453. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1454. choosing restriction sites - (reply: 5)
1455. Tag insertion - (reply: 5)
1456. Transduction - (reply: 3)
1457. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1458. my colonies don't have the insert, how is this possible? - (reply: 7)
1459. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1460. PCR problem from transformed TOPO TA vector - (reply: 3)
1461. nondenaturing PAGE to separate dna - (reply: 4)
1462. why sometimes big or small colonies? - (reply: 16)
1463. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1464. wrong bands after T-vector cloning - (reply: 3)
1465. urgent help regarding wanner method trouble shooting - (reply: 2)
1466. two step digestion - (reply: 6)
1467. Storage of invitrogen top10 cells - (reply: 11)
1468. Self-Ligation of digested vector - (reply: 2)
1469. Help with cloning - (reply: 6)
1470. Ligation issue: Does vector size matter? - (reply: 10)