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Top : New Forum Archives (2009-): : Molecular-Cloning
1441. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1442. A gateway cloning expression vector question - (reply: 1)
1443. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1444. Promoter/gene cloning question - (reply: 7)
1445. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1446. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1447. vivo expression technology - ivet (reply: 25)
1448. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
1449. insert is missing after TA cloning - TA cloning (reply: 6)
1450. glycerol stock - shelf life (reply: 5)
1451. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1452. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1453. cloning problem - molecular cloning (reply: 1)
1454. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1455. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1456. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1457. choosing restriction sites - (reply: 5)
1458. Tag insertion - (reply: 5)
1459. Transduction - (reply: 3)
1460. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1461. my colonies don't have the insert, how is this possible? - (reply: 7)
1462. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1463. PCR problem from transformed TOPO TA vector - (reply: 3)
1464. nondenaturing PAGE to separate dna - (reply: 4)
1465. why sometimes big or small colonies? - (reply: 16)
1466. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1467. wrong bands after T-vector cloning - (reply: 3)
1468. urgent help regarding wanner method trouble shooting - (reply: 2)
1469. two step digestion - (reply: 6)
1470. Storage of invitrogen top10 cells - (reply: 11)