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Top : New Forum Archives (2009-): : Molecular-Cloning
1441. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1442. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1443. Ligation trouble continues - subcloning ligation failure (reply: 9)
1444. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1445. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1446. colony PCR - (reply: 8)
1447. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1448. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1449. Truncated sequence - (reply: 1)
1450. gateway cloning - (reply: 3)
1451. Purifying and cloning 69nt product - (reply: 5)
1452. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1453. ligatin issues - (reply: 1)
1454. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1455. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1456. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1457. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1458. A gateway cloning expression vector question - (reply: 1)
1459. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1460. Promoter/gene cloning question - (reply: 7)
1461. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1462. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1463. vivo expression technology - ivet (reply: 25)
1464. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
1465. insert is missing after TA cloning - TA cloning (reply: 6)
1466. glycerol stock - shelf life (reply: 5)
1467. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1468. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1469. cloning problem - molecular cloning (reply: 1)
1470. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)