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Top : New Forum Archives (2009-): : Molecular-Cloning
1441. gateway cloning - (reply: 3)
1442. Purifying and cloning 69nt product - (reply: 5)
1443. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1444. ligatin issues - (reply: 1)
1445. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1446. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1447. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1448. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1449. A gateway cloning expression vector question - (reply: 1)
1450. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1451. Promoter/gene cloning question - (reply: 7)
1452. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1453. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1454. vivo expression technology - ivet (reply: 25)
1455. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
1456. insert is missing after TA cloning - TA cloning (reply: 6)
1457. glycerol stock - shelf life (reply: 5)
1458. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1459. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1460. cloning problem - molecular cloning (reply: 1)
1461. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1462. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1463. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1464. choosing restriction sites - (reply: 5)
1465. Tag insertion - (reply: 5)
1466. Transduction - (reply: 3)
1467. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1468. my colonies don't have the insert, how is this possible? - (reply: 7)
1469. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1470. PCR problem from transformed TOPO TA vector - (reply: 3)