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211. Swich selection in plasmid - (reply: 3)
212. Sub-cloning and transformation - (reply: 17)
213. cloning without phosphorylation - (reply: 7)
214. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
215. A good vector to make gene library? - (reply: 5)
216. problems with midiprep - (reply: 3)
217. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
218. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
219. 900bp insertion into PIRESnEO,problem - (reply: 2)
220. fusion of cDNA expressing protein with gfp - (reply: 1)
221. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
222. Problem with plasmids transformation - (reply: 6)
223. Cloning genetic sequences of proteins. - (reply: 1)
224. problem of restriction enzyme digestion - (reply: 3)
225. Site-directed, PCR works but no colonies - (reply: 2)
226. Simoltanious Multi-restriction digestion - (reply: 3)
227. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
228. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
229. Restriction digestion problems - (reply: 3)
230. should I do blunt end my pcr product before ligation - (reply: 2)
231. Maxiprep of freeze/thawed pellet. - (reply: 1)
232. Digest with AarI and Pml I - (reply: 1)
233. Help with CGL gene cloning problems - (reply: 1)
234. screening for GPF - (reply: 10)
235. Creating a pGEM-T easy + insert sequence map - (reply: 1)
236. where to get vector overexpressing caveolin-1? - (reply: 2)
237. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
238. LZRS vector details - (reply: 1)
239. 12.4kb vector 6.7kb insert - (reply: 2)
240. Stable transfection - (reply: 2)
212. Sub-cloning and transformation - (reply: 17)
213. cloning without phosphorylation - (reply: 7)
214. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
215. A good vector to make gene library? - (reply: 5)
216. problems with midiprep - (reply: 3)
217. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
218. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
219. 900bp insertion into PIRESnEO,problem - (reply: 2)
220. fusion of cDNA expressing protein with gfp - (reply: 1)
221. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
222. Problem with plasmids transformation - (reply: 6)
223. Cloning genetic sequences of proteins. - (reply: 1)
224. problem of restriction enzyme digestion - (reply: 3)
225. Site-directed, PCR works but no colonies - (reply: 2)
226. Simoltanious Multi-restriction digestion - (reply: 3)
227. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
228. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
229. Restriction digestion problems - (reply: 3)
230. should I do blunt end my pcr product before ligation - (reply: 2)
231. Maxiprep of freeze/thawed pellet. - (reply: 1)
232. Digest with AarI and Pml I - (reply: 1)
233. Help with CGL gene cloning problems - (reply: 1)
234. screening for GPF - (reply: 10)
235. Creating a pGEM-T easy + insert sequence map - (reply: 1)
236. where to get vector overexpressing caveolin-1? - (reply: 2)
237. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
238. LZRS vector details - (reply: 1)
239. 12.4kb vector 6.7kb insert - (reply: 2)
240. Stable transfection - (reply: 2)