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Top : New Forum Archives (2009-): : Molecular-Cloning
211. How efficient is the 2A sequence - (reply: 2)
212. Transformation keeps failing - (reply: 2)
213. High background issue - (reply: 5)
214. Pcr primers - (reply: 7)
215. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
216. Gateway vectors - (reply: 3)
217. question on gateway - (reply: 1)
218. Cloning problem still not solved - (reply: 2)
219. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
220. Insert 30nt at C-terminal - (reply: 1)
221. How to store a ligation reaction over the weekend? - (reply: 3)
222. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
223. How homologous do homology arms need to be? - (reply: 3)
224. cloning pEGFP-N1 - (reply: 1)
225. Loss of DNA after restriction digestion cleanup - (reply: 2)
226. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
227. separating mixture of plamids - (reply: 7)
228. Kozak sequence and mammalian expression vector - (reply: 3)
229. GFP vector - (reply: 1)
230. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
231. no colony - (reply: 3)
232. Cloning beginner and clueless! - (reply: 1)
233. Which epitope tag to use for ChIP? - (reply: 1)
234. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
235. Ligation-transformation...Never faced such problem ever - (reply: 6)
236. Can multiple genes follow only one promoter? - (reply: 1)
237. Restriction Digest Question - (reply: 4)
238. Problem with Large vector & very small insert - (reply: 1)
239. Storage life of plasmid DNA in water - (reply: 5)
240. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)