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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Single codon insert - (reply: 3)
212. cloning- ligation - (reply: 3)
213. Sticky and blunt ligation issue - (reply: 6)
214. Handling DEPC under nitrogen/argon - (reply: 3)
215. Incubation period after heat shock at transformation--reason? - (reply: 3)
216. PCR product sequencing - (reply: 3)
217. What is the optimal time for transfection efficiency measuring? - (reply: 1)
218. i couldn't get the band of target gene after ligation - (reply: 9)
219. Minimum homology bases needed for homologous recombination - (reply: 1)
220. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
221. Expansion of commercial competent bacterial cell lines? - (reply: 2)
222. Manipulation of target DNA fragments - (reply: 1)
223. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
224. Appearance of a salty plasmid preparation - (reply: 1)
225. origami bacteria transformation - (reply: 5)
226. Smallest mRNA length that can be translated - (reply: 3)
227. wrong insert? right insert?? - (reply: 2)
228. Help~ Incorrect constructs in cloning - (reply: 3)
229. Problems with ligation and transformation - (reply: 2)
230. Transformation of Restricted plasmid - (reply: 3)
231. Scrambled sequence? - (reply: 3)
232. Why is my insert missing from my plasmid? - (reply: 4)
233. PCR Profile for ligation - (reply: 3)
234. Blunt end is ligating to sticky end? - (reply: 2)
235. SNP in the promoter region - (reply: 1)
236. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
237. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
238. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
239. New ordered Restriction Enzymes not working, need help! - (reply: 2)
240. Will a Lac promoter sequence interrupt transcription? - (reply: 6)