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211. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
212. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
213. restriction enzymes/sites to avoid - (reply: 4)
214. Using pBABE plasmids for regular transfection - (reply: 1)
215. Ubiquitin promoter cloning - (reply: 1)
216. Cloning two genes in the same plasmid with one promotor - (reply: 4)
217. Sending a TOPO-construct for sequencing? - (reply: 1)
218. Is this counter-productive cloning or not? - (reply: 8)
219. Colony screening - (reply: 1)
220. Failed cloning for 1month - TOPO - (reply: 7)
221. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
222. Which method for DNA purification b4 cloning - (reply: 4)
223. Purification for TOPO - (reply: 1)
224. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
225. Failed TOPO cloning - what happened? - (reply: 9)
226. TOPO cloning after Phusion-PCR - (reply: 1)
227. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
228. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
229. Deleting 7Mbp chromosomal region - (reply: 3)
230. Electrophoresis samples and volumes - (reply: 1)
231. Gene cloning with unknown sequence - (reply: 6)
232. Checking for efficieny competent cells - (reply: 1)
233. Troubles with Fusion PCR - (reply: 1)
234. Does this look alright? - (reply: 3)
235. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
236. Cloning pDsRed-Monomer-N1 - (reply: 1)
237. forgot to heat shock - (reply: 6)
238. Ligation left for weekend at room temp - (reply: 4)
239. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
240. Problem in plasmid digestion after transformation - (reply: 4)
212. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
213. restriction enzymes/sites to avoid - (reply: 4)
214. Using pBABE plasmids for regular transfection - (reply: 1)
215. Ubiquitin promoter cloning - (reply: 1)
216. Cloning two genes in the same plasmid with one promotor - (reply: 4)
217. Sending a TOPO-construct for sequencing? - (reply: 1)
218. Is this counter-productive cloning or not? - (reply: 8)
219. Colony screening - (reply: 1)
220. Failed cloning for 1month - TOPO - (reply: 7)
221. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
222. Which method for DNA purification b4 cloning - (reply: 4)
223. Purification for TOPO - (reply: 1)
224. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
225. Failed TOPO cloning - what happened? - (reply: 9)
226. TOPO cloning after Phusion-PCR - (reply: 1)
227. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
228. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
229. Deleting 7Mbp chromosomal region - (reply: 3)
230. Electrophoresis samples and volumes - (reply: 1)
231. Gene cloning with unknown sequence - (reply: 6)
232. Checking for efficieny competent cells - (reply: 1)
233. Troubles with Fusion PCR - (reply: 1)
234. Does this look alright? - (reply: 3)
235. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
236. Cloning pDsRed-Monomer-N1 - (reply: 1)
237. forgot to heat shock - (reply: 6)
238. Ligation left for weekend at room temp - (reply: 4)
239. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
240. Problem in plasmid digestion after transformation - (reply: 4)