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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Please comment on my Cloning Plan - (reply: 1)
212. Gibson/SLIC question - (reply: 1)
213. How to determine where, in the genome, my transgene inserted - (reply: 4)
214. no colonies, ever! - (reply: 5)
215. fluorescent tag on membrane protein - (reply: 2)
216. How can addgene sell plasmids with patented technology - (reply: 2)
217. no insert issue - (reply: 9)
218. Considerations on the design of a transgene - (reply: 1)
219. How to obtain Vectors used in articles - (reply: 5)
220. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
221. How efficient is the 2A sequence - (reply: 2)
222. Transformation keeps failing - (reply: 2)
223. High background issue - (reply: 5)
224. Pcr primers - (reply: 7)
225. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
226. Gateway vectors - (reply: 3)
227. question on gateway - (reply: 1)
228. Cloning problem still not solved - (reply: 2)
229. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
230. Insert 30nt at C-terminal - (reply: 1)
231. How to store a ligation reaction over the weekend? - (reply: 3)
232. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
233. How homologous do homology arms need to be? - (reply: 3)
234. cloning pEGFP-N1 - (reply: 1)
235. Loss of DNA after restriction digestion cleanup - (reply: 2)
236. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
237. separating mixture of plamids - (reply: 7)
238. Kozak sequence and mammalian expression vector - (reply: 3)
239. GFP vector - (reply: 1)
240. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)