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Top : New Forum Archives (2009-): : Molecular-Cloning
211. colony PCR after transformation - (reply: 1)
212. For promoter assay, which vector I should use? - (reply: 5)
213. Sequencing of the cloned plasmid - (reply: 5)
214. 4 way cloning into TA Vector - (reply: 10)
215. I get band double the size what does it mean - (reply: 3)
216. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
217. Neeed Help , cloning Confirmation ?! - (reply: 5)
218. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
219. Can I quantify restricted plasmid and oligos? - (reply: 3)
220. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
221. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
222. TOPO TA ligation problems - (reply: 6)
223. Luciferase assay problem - (reply: 2)
224. Double digestion with BamHI and BglII - (reply: 4)
225. nicked linear DNA - (reply: 5)
226. Selection of E.coli competent cells - (reply: 2)
227. Double strand cdna synthysis - (reply: 4)
228. mutagenic primers with very high GC content. - (reply: 3)
229. TA Cloning of ds cDNA - (reply: 6)
230. Promoter distance from ATG - (reply: 3)
231. Measuring Fluorescene of RFP - (reply: 7)
232. Smallest possible insert size for Ligation reaction - (reply: 2)
233. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
234. Extraction of DNA from termite gut flagellates - (reply: 7)
235. Enzymes and buffers from different companies - (reply: 6)
236. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
237. faint band after plasmid extraction - (reply: 7)
238. Adaptor ligation - (reply: 9)
239. self ligation - (reply: 1)
240. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)