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Top : New Forum Archives (2009-): : Molecular-Cloning
211. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
212. Problem in plasmid digestion after transformation - (reply: 4)
213. Lac Promoter activity in P. aeruginosa - (reply: 1)
214. Resctriction analysis - (reply: 5)
215. Please comment on my Cloning Plan - (reply: 1)
216. Gibson/SLIC question - (reply: 1)
217. How to determine where, in the genome, my transgene inserted - (reply: 4)
218. no colonies, ever! - (reply: 5)
219. fluorescent tag on membrane protein - (reply: 2)
220. How can addgene sell plasmids with patented technology - (reply: 2)
221. no insert issue - (reply: 9)
222. Considerations on the design of a transgene - (reply: 1)
223. How to obtain Vectors used in articles - (reply: 5)
224. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
225. How efficient is the 2A sequence - (reply: 2)
226. Transformation keeps failing - (reply: 2)
227. High background issue - (reply: 5)
228. Pcr primers - (reply: 7)
229. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
230. Gateway vectors - (reply: 3)
231. question on gateway - (reply: 1)
232. Cloning problem still not solved - (reply: 2)
233. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
234. Insert 30nt at C-terminal - (reply: 1)
235. How to store a ligation reaction over the weekend? - (reply: 3)
236. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
237. How homologous do homology arms need to be? - (reply: 3)
238. cloning pEGFP-N1 - (reply: 1)
239. Loss of DNA after restriction digestion cleanup - (reply: 2)
240. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)