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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
212. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
213. Deleting 7Mbp chromosomal region - (reply: 3)
214. Electrophoresis samples and volumes - (reply: 1)
215. Gene cloning with unknown sequence - (reply: 6)
216. Checking for efficieny competent cells - (reply: 1)
217. Troubles with Fusion PCR - (reply: 1)
218. Does this look alright? - (reply: 3)
219. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
220. Cloning pDsRed-Monomer-N1 - (reply: 1)
221. forgot to heat shock - (reply: 6)
222. Ligation left for weekend at room temp - (reply: 4)
223. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
224. Problem in plasmid digestion after transformation - (reply: 4)
225. Lac Promoter activity in P. aeruginosa - (reply: 1)
226. Resctriction analysis - (reply: 5)
227. Please comment on my Cloning Plan - (reply: 1)
228. Gibson/SLIC question - (reply: 1)
229. How to determine where, in the genome, my transgene inserted - (reply: 4)
230. no colonies, ever! - (reply: 5)
231. fluorescent tag on membrane protein - (reply: 2)
232. How can addgene sell plasmids with patented technology - (reply: 2)
233. no insert issue - (reply: 9)
234. Considerations on the design of a transgene - (reply: 1)
235. How to obtain Vectors used in articles - (reply: 5)
236. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
237. How efficient is the 2A sequence - (reply: 2)
238. Transformation keeps failing - (reply: 2)
239. High background issue - (reply: 5)
240. Pcr primers - (reply: 7)