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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
212. Failed TOPO cloning - what happened? - (reply: 9)
213. TOPO cloning after Phusion-PCR - (reply: 1)
214. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
215. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
216. Deleting 7Mbp chromosomal region - (reply: 3)
217. Electrophoresis samples and volumes - (reply: 1)
218. Gene cloning with unknown sequence - (reply: 6)
219. Checking for efficieny competent cells - (reply: 1)
220. Troubles with Fusion PCR - (reply: 1)
221. Does this look alright? - (reply: 3)
222. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
223. Cloning pDsRed-Monomer-N1 - (reply: 1)
224. forgot to heat shock - (reply: 6)
225. Ligation left for weekend at room temp - (reply: 4)
226. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
227. Problem in plasmid digestion after transformation - (reply: 4)
228. Lac Promoter activity in P. aeruginosa - (reply: 1)
229. Resctriction analysis - (reply: 5)
230. Please comment on my Cloning Plan - (reply: 1)
231. Gibson/SLIC question - (reply: 1)
232. How to determine where, in the genome, my transgene inserted - (reply: 4)
233. no colonies, ever! - (reply: 5)
234. fluorescent tag on membrane protein - (reply: 2)
235. How can addgene sell plasmids with patented technology - (reply: 2)
236. no insert issue - (reply: 9)
237. Considerations on the design of a transgene - (reply: 1)
238. How to obtain Vectors used in articles - (reply: 5)
239. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
240. How efficient is the 2A sequence - (reply: 2)