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Top : New Forum Archives (2009-): : Molecular-Cloning
211. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
212. Cloning and expression problem - (reply: 5)
213. How do silicates adhere nucleic acid? - (reply: 1)
214. Ligation of PCR fragments - (reply: 11)
215. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
216. What plasmid sizes do you work with for SDM? - (reply: 1)
217. cloning help; high salt level in vector - (reply: 4)
218. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
219. problem with gateway cloning: missing part of insert - (reply: 2)
220. T7 and M13 primers two band amplification - (reply: 3)
221. Type of polymerases compatible for TA cloning - (reply: 7)
222. Running undigested plasmids on gel - (reply: 2)
223. Molecular Cloning Sambrook - (reply: 1)
224. What is the membrane made of in DNA purification kits - (reply: 3)
225. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
226. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
227. Removing Introns from a DNA sequence - (reply: 1)
228. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
229. without RE site in PCR product - (reply: 5)
230. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
231. excision of fragment during cloning - (reply: 4)
232. Unusual cloning issue... anyone else?? - (reply: 4)
233. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
234. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
235. How to avoid RNA contamination in DNA samples:? - (reply: 1)
236. Wavy Lines in Gel - (reply: 2)
237. Question Relating to LacZ Disruption - (reply: 4)
238. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
239. Site directed mutagenesis - (reply: 5)
240. PCR insert - in frame? - (reply: 2)