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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Change nucleotide - (reply: 1)
212. Colonies grown without plasmid - (reply: 7)
213. unwanted band - (reply: 5)
214. Colony PCR positive and Digestion negative????? - (reply: 11)
215. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
216. Plasmid on blotting paper - (reply: 1)
217. Dimerization of PCR product - (reply: 4)
218. How to generate Lentivirus? - (reply: 4)
219. Do genes always need to be cloned in the "right direction"? - (reply: 3)
220. Gel purification (DNA) - (reply: 1)
221. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
222. Cloning and expression problem - (reply: 5)
223. How do silicates adhere nucleic acid? - (reply: 1)
224. Ligation of PCR fragments - (reply: 11)
225. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
226. What plasmid sizes do you work with for SDM? - (reply: 1)
227. cloning help; high salt level in vector - (reply: 4)
228. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
229. problem with gateway cloning: missing part of insert - (reply: 2)
230. T7 and M13 primers two band amplification - (reply: 3)
231. Type of polymerases compatible for TA cloning - (reply: 7)
232. Running undigested plasmids on gel - (reply: 2)
233. Molecular Cloning Sambrook - (reply: 1)
234. What is the membrane made of in DNA purification kits - (reply: 3)
235. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
236. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
237. Removing Introns from a DNA sequence - (reply: 1)
238. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
239. without RE site in PCR product - (reply: 5)
240. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)