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Top : New Forum Archives (2009-): : Molecular-Cloning
211. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
212. without RE site in PCR product - (reply: 5)
213. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
214. excision of fragment during cloning - (reply: 4)
215. Unusual cloning issue... anyone else?? - (reply: 4)
216. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
217. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
218. How to avoid RNA contamination in DNA samples:? - (reply: 1)
219. Wavy Lines in Gel - (reply: 2)
220. Question Relating to LacZ Disruption - (reply: 4)
221. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
222. Site directed mutagenesis - (reply: 5)
223. PCR insert - in frame? - (reply: 2)
224. Whole plasmid amplification by PCR - (reply: 2)
225. colony PCR after transformation - (reply: 1)
226. For promoter assay, which vector I should use? - (reply: 5)
227. Sequencing of the cloned plasmid - (reply: 5)
228. 4 way cloning into TA Vector - (reply: 10)
229. I get band double the size what does it mean - (reply: 3)
230. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
231. Neeed Help , cloning Confirmation ?! - (reply: 5)
232. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
233. Can I quantify restricted plasmid and oligos? - (reply: 3)
234. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
235. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
236. TOPO TA ligation problems - (reply: 6)
237. Luciferase assay problem - (reply: 2)
238. Double digestion with BamHI and BglII - (reply: 4)
239. nicked linear DNA - (reply: 5)
240. Selection of E.coli competent cells - (reply: 2)