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Top : New Forum Archives (2009-): : Molecular-Cloning
211. How to determine where, in the genome, my transgene inserted - (reply: 4)
212. no colonies, ever! - (reply: 5)
213. fluorescent tag on membrane protein - (reply: 2)
214. How can addgene sell plasmids with patented technology - (reply: 2)
215. no insert issue - (reply: 9)
216. Considerations on the design of a transgene - (reply: 1)
217. How to obtain Vectors used in articles - (reply: 5)
218. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
219. How efficient is the 2A sequence - (reply: 2)
220. Transformation keeps failing - (reply: 2)
221. High background issue - (reply: 5)
222. Pcr primers - (reply: 7)
223. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
224. Gateway vectors - (reply: 3)
225. question on gateway - (reply: 1)
226. Cloning problem still not solved - (reply: 2)
227. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
228. Insert 30nt at C-terminal - (reply: 1)
229. How to store a ligation reaction over the weekend? - (reply: 3)
230. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
231. How homologous do homology arms need to be? - (reply: 3)
232. cloning pEGFP-N1 - (reply: 1)
233. Loss of DNA after restriction digestion cleanup - (reply: 2)
234. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
235. separating mixture of plamids - (reply: 7)
236. Kozak sequence and mammalian expression vector - (reply: 3)
237. GFP vector - (reply: 1)
238. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
239. no colony - (reply: 3)
240. Cloning beginner and clueless! - (reply: 1)