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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Purification for TOPO - (reply: 1)
212. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
213. Failed TOPO cloning - what happened? - (reply: 9)
214. TOPO cloning after Phusion-PCR - (reply: 1)
215. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
216. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
217. Deleting 7Mbp chromosomal region - (reply: 3)
218. Electrophoresis samples and volumes - (reply: 1)
219. Gene cloning with unknown sequence - (reply: 6)
220. Checking for efficieny competent cells - (reply: 1)
221. Troubles with Fusion PCR - (reply: 1)
222. Does this look alright? - (reply: 3)
223. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
224. Cloning pDsRed-Monomer-N1 - (reply: 1)
225. forgot to heat shock - (reply: 6)
226. Ligation left for weekend at room temp - (reply: 4)
227. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
228. Problem in plasmid digestion after transformation - (reply: 4)
229. Lac Promoter activity in P. aeruginosa - (reply: 1)
230. Resctriction analysis - (reply: 5)
231. Please comment on my Cloning Plan - (reply: 1)
232. Gibson/SLIC question - (reply: 1)
233. How to determine where, in the genome, my transgene inserted - (reply: 4)
234. no colonies, ever! - (reply: 5)
235. fluorescent tag on membrane protein - (reply: 2)
236. How can addgene sell plasmids with patented technology - (reply: 2)
237. no insert issue - (reply: 9)
238. Considerations on the design of a transgene - (reply: 1)
239. How to obtain Vectors used in articles - (reply: 5)
240. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)