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Top : New Forum Archives (2009-): : Molecular-Cloning
211. PCR Profile for ligation - (reply: 3)
212. Blunt end is ligating to sticky end? - (reply: 2)
213. SNP in the promoter region - (reply: 1)
214. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
215. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
216. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
217. New ordered Restriction Enzymes not working, need help! - (reply: 2)
218. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
219. Transfection but no expression - (reply: 4)
220. Change nucleotide - (reply: 1)
221. Colonies grown without plasmid - (reply: 7)
222. unwanted band - (reply: 5)
223. Colony PCR positive and Digestion negative????? - (reply: 11)
224. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
225. Plasmid on blotting paper - (reply: 1)
226. Dimerization of PCR product - (reply: 4)
227. How to generate Lentivirus? - (reply: 4)
228. Do genes always need to be cloned in the "right direction"? - (reply: 3)
229. Gel purification (DNA) - (reply: 1)
230. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
231. Cloning and expression problem - (reply: 5)
232. How do silicates adhere nucleic acid? - (reply: 1)
233. Ligation of PCR fragments - (reply: 11)
234. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
235. What plasmid sizes do you work with for SDM? - (reply: 1)
236. cloning help; high salt level in vector - (reply: 4)
237. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
238. problem with gateway cloning: missing part of insert - (reply: 2)
239. T7 and M13 primers two band amplification - (reply: 3)
240. Type of polymerases compatible for TA cloning - (reply: 7)