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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Resctriction analysis - (reply: 5)
212. Please comment on my Cloning Plan - (reply: 1)
213. Gibson/SLIC question - (reply: 1)
214. How to determine where, in the genome, my transgene inserted - (reply: 4)
215. no colonies, ever! - (reply: 5)
216. fluorescent tag on membrane protein - (reply: 2)
217. How can addgene sell plasmids with patented technology - (reply: 2)
218. no insert issue - (reply: 9)
219. Considerations on the design of a transgene - (reply: 1)
220. How to obtain Vectors used in articles - (reply: 5)
221. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
222. How efficient is the 2A sequence - (reply: 2)
223. Transformation keeps failing - (reply: 2)
224. High background issue - (reply: 5)
225. Pcr primers - (reply: 7)
226. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
227. Gateway vectors - (reply: 3)
228. question on gateway - (reply: 1)
229. Cloning problem still not solved - (reply: 2)
230. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
231. Insert 30nt at C-terminal - (reply: 1)
232. How to store a ligation reaction over the weekend? - (reply: 3)
233. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
234. How homologous do homology arms need to be? - (reply: 3)
235. cloning pEGFP-N1 - (reply: 1)
236. Loss of DNA after restriction digestion cleanup - (reply: 2)
237. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
238. separating mixture of plamids - (reply: 7)
239. Kozak sequence and mammalian expression vector - (reply: 3)
240. GFP vector - (reply: 1)