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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Does this look alright? - (reply: 3)
212. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
213. Cloning pDsRed-Monomer-N1 - (reply: 1)
214. forgot to heat shock - (reply: 6)
215. Ligation left for weekend at room temp - (reply: 4)
216. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
217. Problem in plasmid digestion after transformation - (reply: 4)
218. Lac Promoter activity in P. aeruginosa - (reply: 1)
219. Resctriction analysis - (reply: 5)
220. Please comment on my Cloning Plan - (reply: 1)
221. Gibson/SLIC question - (reply: 1)
222. How to determine where, in the genome, my transgene inserted - (reply: 4)
223. no colonies, ever! - (reply: 5)
224. fluorescent tag on membrane protein - (reply: 2)
225. How can addgene sell plasmids with patented technology - (reply: 2)
226. no insert issue - (reply: 9)
227. Considerations on the design of a transgene - (reply: 1)
228. How to obtain Vectors used in articles - (reply: 5)
229. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
230. How efficient is the 2A sequence - (reply: 2)
231. Transformation keeps failing - (reply: 2)
232. High background issue - (reply: 5)
233. Pcr primers - (reply: 7)
234. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
235. Gateway vectors - (reply: 3)
236. question on gateway - (reply: 1)
237. Cloning problem still not solved - (reply: 2)
238. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
239. Insert 30nt at C-terminal - (reply: 1)
240. How to store a ligation reaction over the weekend? - (reply: 3)