Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
211. Unusual cloning issue... anyone else?? - (reply: 4)
212. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
213. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
214. How to avoid RNA contamination in DNA samples:? - (reply: 1)
215. Wavy Lines in Gel - (reply: 2)
216. Question Relating to LacZ Disruption - (reply: 4)
217. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
218. Site directed mutagenesis - (reply: 5)
219. PCR insert - in frame? - (reply: 2)
220. Whole plasmid amplification by PCR - (reply: 2)
221. colony PCR after transformation - (reply: 1)
222. For promoter assay, which vector I should use? - (reply: 5)
223. Sequencing of the cloned plasmid - (reply: 5)
224. 4 way cloning into TA Vector - (reply: 10)
225. I get band double the size what does it mean - (reply: 3)
226. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
227. Neeed Help , cloning Confirmation ?! - (reply: 5)
228. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
229. Can I quantify restricted plasmid and oligos? - (reply: 3)
230. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
231. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
232. TOPO TA ligation problems - (reply: 6)
233. Luciferase assay problem - (reply: 2)
234. Double digestion with BamHI and BglII - (reply: 4)
235. nicked linear DNA - (reply: 5)
236. Selection of E.coli competent cells - (reply: 2)
237. Double strand cdna synthysis - (reply: 4)
238. mutagenic primers with very high GC content. - (reply: 3)
239. TA Cloning of ds cDNA - (reply: 6)
240. Promoter distance from ATG - (reply: 3)