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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Is this counter-productive cloning or not? - (reply: 8)
212. Colony screening - (reply: 1)
213. Failed cloning for 1month - TOPO - (reply: 7)
214. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
215. Which method for DNA purification b4 cloning - (reply: 4)
216. Purification for TOPO - (reply: 1)
217. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
218. Failed TOPO cloning - what happened? - (reply: 9)
219. TOPO cloning after Phusion-PCR - (reply: 1)
220. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
221. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
222. Deleting 7Mbp chromosomal region - (reply: 3)
223. Electrophoresis samples and volumes - (reply: 1)
224. Gene cloning with unknown sequence - (reply: 6)
225. Checking for efficieny competent cells - (reply: 1)
226. Troubles with Fusion PCR - (reply: 1)
227. Does this look alright? - (reply: 3)
228. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
229. Cloning pDsRed-Monomer-N1 - (reply: 1)
230. forgot to heat shock - (reply: 6)
231. Ligation left for weekend at room temp - (reply: 4)
232. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
233. Problem in plasmid digestion after transformation - (reply: 4)
234. Lac Promoter activity in P. aeruginosa - (reply: 1)
235. Resctriction analysis - (reply: 5)
236. Please comment on my Cloning Plan - (reply: 1)
237. Gibson/SLIC question - (reply: 1)
238. How to determine where, in the genome, my transgene inserted - (reply: 4)
239. no colonies, ever! - (reply: 5)
240. fluorescent tag on membrane protein - (reply: 2)