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Top : New Forum Archives (2009-): : Molecular-Cloning
211. Which epitope tag to use for ChIP? - (reply: 1)
212. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
213. Ligation-transformation...Never faced such problem ever - (reply: 6)
214. Can multiple genes follow only one promoter? - (reply: 1)
215. Restriction Digest Question - (reply: 4)
216. Problem with Large vector & very small insert - (reply: 1)
217. Storage life of plasmid DNA in water - (reply: 5)
218. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
219. Site-directed mutagenesis failed - (reply: 20)
220. Insert & vector too dilute to perform ligation - (reply: 3)
221. Cloning Problems - (reply: 2)
222. Suitable templates for in vitro transcription - (reply: 1)
223. Different ways to ensure the successful cloning? - (reply: 2)
224. Plasmid DNA digestion problem - (reply: 3)
225. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
226. Single codon insert - (reply: 3)
227. cloning- ligation - (reply: 3)
228. Sticky and blunt ligation issue - (reply: 6)
229. Handling DEPC under nitrogen/argon - (reply: 3)
230. Incubation period after heat shock at transformation--reason? - (reply: 3)
231. PCR product sequencing - (reply: 3)
232. What is the optimal time for transfection efficiency measuring? - (reply: 1)
233. i couldn't get the band of target gene after ligation - (reply: 9)
234. Minimum homology bases needed for homologous recombination - (reply: 1)
235. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
236. Expansion of commercial competent bacterial cell lines? - (reply: 2)
237. Manipulation of target DNA fragments - (reply: 1)
238. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
239. Appearance of a salty plasmid preparation - (reply: 1)
240. origami bacteria transformation - (reply: 5)