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Top : New Forum Archives (2009-): : Molecular-Cloning
211. forgot to heat shock - (reply: 6)
212. Ligation left for weekend at room temp - (reply: 4)
213. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
214. Problem in plasmid digestion after transformation - (reply: 4)
215. Lac Promoter activity in P. aeruginosa - (reply: 1)
216. Resctriction analysis - (reply: 5)
217. Please comment on my Cloning Plan - (reply: 1)
218. Gibson/SLIC question - (reply: 1)
219. How to determine where, in the genome, my transgene inserted - (reply: 4)
220. no colonies, ever! - (reply: 5)
221. fluorescent tag on membrane protein - (reply: 2)
222. How can addgene sell plasmids with patented technology - (reply: 2)
223. no insert issue - (reply: 9)
224. Considerations on the design of a transgene - (reply: 1)
225. How to obtain Vectors used in articles - (reply: 5)
226. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
227. How efficient is the 2A sequence - (reply: 2)
228. Transformation keeps failing - (reply: 2)
229. High background issue - (reply: 5)
230. Pcr primers - (reply: 7)
231. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
232. Gateway vectors - (reply: 3)
233. question on gateway - (reply: 1)
234. Cloning problem still not solved - (reply: 2)
235. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
236. Insert 30nt at C-terminal - (reply: 1)
237. How to store a ligation reaction over the weekend? - (reply: 3)
238. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
239. How homologous do homology arms need to be? - (reply: 3)
240. cloning pEGFP-N1 - (reply: 1)