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Top : New Forum Archives (2009-): : Molecular-Cloning
751. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
752. Cloning App Released from Life Technologies - (reply: 2)
753. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
754. Dissolve IPTG in EtOH - (reply: 4)
755. problem with transient transfection - (reply: 6)
756. Recombinant construct looks smaller than empty vector - (reply: 3)
757. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
758. hygromycin concentration for e-coli selection - (reply: 1)
759. orientation of promoter into viral construct - (reply: 1)
760. problems with PCR confirmation of insert . HELP ! - (reply: 1)
761. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
762. Loss of promoter during ligation - (reply: 2)
763. Enzyme restriction - (reply: 8)
764. Colony PCR Problem!!!! - (reply: 5)
765. Removing unwanted sequence from pUC19 - (reply: 4)
766. transformation fails, bacteria grows in control plate - (reply: 6)
767. competentce cell, cloning - (reply: 2)
768. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
769. co-transformation - (reply: 3)
770. pcr clean-up fail? - (reply: 1)
771. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
772. sequential cloning of multiple PCR products - how to do? (reply: 3)
773. Cloning large gene - (reply: 3)
774. Glycosilated peptide's cDNA cloning - (reply: 3)
775. pWM91 cloning problem - (reply: 4)
776. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
777. Transformation - Transformation (reply: 1)
778. how to add 3XHA to the N-terminal of a protein - (reply: 5)
779. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
780. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)