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Top : New Forum Archives (2009-): : Molecular-Cloning
751. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
752. Template DNA (plasmid) concentration for PCR - (reply: 4)
753. Ligation of 16kb PCR product - (reply: 5)
754. stable transfection - (reply: 1)
755. How to choose the right plasmids for certain types of cell? - (reply: 1)
756. Ligation needed after SDM with PfuX? - (reply: 3)
757. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
758. Cloning promoter (overlap with another gene!) - (reply: 3)
759. bacterial transfeomation - (reply: 2)
760. Loss of insert? - (reply: 1)
761. Sequencing trouble - (reply: 7)
762. Platinum-A cell trasnfection toruble - (reply: 1)
763. Cloning a promoter - (reply: 2)
764. pcDNA 3.1 His Expression - (reply: 2)
765. Cloning two fragments into a vector - (reply: 3)
766. confirmation of insert in to vector - (reply: 3)
767. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
768. Question about Molar ratio - (reply: 4)
769. Determining optimal digestion time for partial digestion - (reply: 2)
770. Ligation problem - (reply: 16)
771. double digestion with sac1, hindIII - (reply: 3)
772. self-ligation Anneled oligos - (reply: 7)
773. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
774. Looking for a GFP plasmid (non-amp) - (reply: 3)
775. clonning problem (recombined by-products) - (reply: 8)
776. Genecloning three types of molecules? - (reply: 11)
777. help in digesting DNA fragment using restriction enzyme - (reply: 2)
778. Tansformation fails ,how can i make it ? - (reply: 3)
779. pelB leader - how to use - (reply: 1)
780. Transformation bacteria heat chock and incubation? - (reply: 2)