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Top : New Forum Archives (2009-): : Molecular-Cloning
751. ligation of pcr product into expression vector - (reply: 4)
752. amp plates containing xgal/iptg with expression vector - (reply: 6)
753. Strange Vector Problems - (reply: 3)
754. white colonies - (reply: 2)
755. how to design a kozak sequence in front of the start codon - (reply: 1)
756. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
757. XhoI digestion - (reply: 12)
758. Lenti vector ligation - (reply: 2)
759. Cloning from a pGEMT Easy vector - (reply: 3)
760. Distance between RE sites for double digestion - (reply: 5)
761. Plasmid compatible with pBAD24 - (reply: 1)
762. I cant get colony - (reply: 3)
763. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
764. Vector with inducible/repressible system - (reply: 1)
765. cloning problem - (reply: 9)
766. Cloning genes into plant vector - (reply: 1)
767. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
768. Three-way ligation in two steps? - (reply: 3)
769. How does circular, uncut pGEM-T migrate? - (reply: 2)
770. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
771. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
772. Cloning App Released from Life Technologies - (reply: 2)
773. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
774. Dissolve IPTG in EtOH - (reply: 4)
775. problem with transient transfection - (reply: 6)
776. Recombinant construct looks smaller than empty vector - (reply: 3)
777. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
778. hygromycin concentration for e-coli selection - (reply: 1)
779. orientation of promoter into viral construct - (reply: 1)
780. problems with PCR confirmation of insert . HELP ! - (reply: 1)