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Top : New Forum Archives (2009-): : Molecular-Cloning
751. Gateway cloning vector compatibility - (reply: 2)
752. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
753. Cloning of IL8-promoter - (reply: 3)
754. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
755. Template DNA (plasmid) concentration for PCR - (reply: 4)
756. Ligation of 16kb PCR product - (reply: 5)
757. stable transfection - (reply: 1)
758. How to choose the right plasmids for certain types of cell? - (reply: 1)
759. Ligation needed after SDM with PfuX? - (reply: 3)
760. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
761. Cloning promoter (overlap with another gene!) - (reply: 3)
762. bacterial transfeomation - (reply: 2)
763. Loss of insert? - (reply: 1)
764. Sequencing trouble - (reply: 7)
765. Platinum-A cell trasnfection toruble - (reply: 1)
766. Cloning a promoter - (reply: 2)
767. pcDNA 3.1 His Expression - (reply: 2)
768. Cloning two fragments into a vector - (reply: 3)
769. confirmation of insert in to vector - (reply: 3)
770. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
771. Question about Molar ratio - (reply: 4)
772. Determining optimal digestion time for partial digestion - (reply: 2)
773. Ligation problem - (reply: 16)
774. double digestion with sac1, hindIII - (reply: 3)
775. self-ligation Anneled oligos - (reply: 7)
776. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
777. Looking for a GFP plasmid (non-amp) - (reply: 3)
778. clonning problem (recombined by-products) - (reply: 8)
779. Genecloning three types of molecules? - (reply: 11)
780. help in digesting DNA fragment using restriction enzyme - (reply: 2)