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Top : New Forum Archives (2009-): : Molecular-Cloning
751. miRNA cloning - (reply: 2)
752. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
753. Single-digested vector shifts downward on gel - (reply: 1)
754. problem in digestion - (reply: 1)
755. Cloning does not work - (reply: 4)
756. Small Colonies After Transformation - (reply: 5)
757. Ligation and transformation problem - (reply: 2)
758. Agrobacterium EHA101 competent cells - (reply: 1)
759. restriction digestion against colony PCR - (reply: 1)
760. BAC for transgenic mice - (reply: 6)
761. Tag proteins for CO-IP and confocal - (reply: 1)
762. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
763. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
764. how long for a double digestion - (reply: 2)
765. Problems with Site-Directed Mutagenesis - (reply: 2)
766. blunt and sticky end clonning temperature - (reply: 2)
767. Most common mistakes - What mistakes are made during cloning? (reply: 7)
768. Luciferase Assay Stim. Concentration? - (reply: 1)
769. Cloning direction / orientation? - (reply: 2)
770. Question about plasmid midiprep - (reply: 1)
771. Does a polyA sequence need to be put in-frame? - (reply: 1)
772. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
773. Problem on growing up bugs - (reply: 8)
774. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
775. pSIM in BL21(DE3) - (reply: 2)
776. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
777. Weird problem in invitromutagenesis - (reply: 3)
778. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
779. RE digestion - (reply: 4)
780. HELP!!! - (reply: 3)