Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
751. 2 bands in blue colony PCR - (reply: 5)
752. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
753. Introduction to vector development - Basics on vectors types and construction (reply: 1)
754. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
755. Targeted mutagenesis using a linear fragment - (reply: 1)
756. Transformation & Minimal Plates - (reply: 5)
757. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
758. Subcloning Problem - (reply: 3)
759. Restriction Enzyme sites in my genes - (reply: 1)
760. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
761. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
762. In-Fusion (Clontech) Reaction buffer - (reply: 1)
763. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
764. MDA-MB-231 cell transfection - (reply: 4)
765. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
766. Break Cell Clumps - prepare single cells for injections (reply: 1)
767. 126pb insert clonnig/ digestion issues - (reply: 3)
768. cloning problem... - (reply: 5)
769. yeast glycerol stock - (reply: 2)
770. yeast transformation - (reply: 1)
771. Problems with ligation and transformation - (reply: 2)
772. very small insert and a very ling vector - (reply: 1)
773. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
774. cDNA clone - (reply: 1)
775. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
776. why these guys use T7 instead of CMV? - for transfection (reply: 2)
777. Electroporation - no colonies (reply: 1)
778. enzymes inactivation - (reply: 3)
779. help: why plasmid yield dropped ever since the first culture - (reply: 2)
780. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)