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Top : New Forum Archives (2009-): : Molecular-Cloning
751. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
752. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
753. Cloning App Released from Life Technologies - (reply: 2)
754. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
755. Dissolve IPTG in EtOH - (reply: 4)
756. problem with transient transfection - (reply: 6)
757. Recombinant construct looks smaller than empty vector - (reply: 3)
758. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
759. hygromycin concentration for e-coli selection - (reply: 1)
760. orientation of promoter into viral construct - (reply: 1)
761. problems with PCR confirmation of insert . HELP ! - (reply: 1)
762. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
763. Loss of promoter during ligation - (reply: 2)
764. Enzyme restriction - (reply: 8)
765. Colony PCR Problem!!!! - (reply: 5)
766. Removing unwanted sequence from pUC19 - (reply: 4)
767. transformation fails, bacteria grows in control plate - (reply: 6)
768. competentce cell, cloning - (reply: 2)
769. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
770. co-transformation - (reply: 3)
771. pcr clean-up fail? - (reply: 1)
772. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
773. sequential cloning of multiple PCR products - how to do? (reply: 3)
774. Cloning large gene - (reply: 3)
775. Glycosilated peptide's cDNA cloning - (reply: 3)
776. pWM91 cloning problem - (reply: 4)
777. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
778. Transformation - Transformation (reply: 1)
779. how to add 3XHA to the N-terminal of a protein - (reply: 5)
780. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)