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Top : New Forum Archives (2009-): : Molecular-Cloning
751. self-ligation Anneled oligos - (reply: 7)
752. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
753. Looking for a GFP plasmid (non-amp) - (reply: 3)
754. clonning problem (recombined by-products) - (reply: 8)
755. Genecloning three types of molecules? - (reply: 11)
756. help in digesting DNA fragment using restriction enzyme - (reply: 2)
757. Tansformation fails ,how can i make it ? - (reply: 3)
758. pelB leader - how to use - (reply: 1)
759. Transformation bacteria heat chock and incubation? - (reply: 2)
760. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
761. ligation of pcr product into expression vector - (reply: 4)
762. amp plates containing xgal/iptg with expression vector - (reply: 6)
763. Strange Vector Problems - (reply: 3)
764. white colonies - (reply: 2)
765. how to design a kozak sequence in front of the start codon - (reply: 1)
766. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
767. XhoI digestion - (reply: 12)
768. Lenti vector ligation - (reply: 2)
769. Cloning from a pGEMT Easy vector - (reply: 3)
770. Distance between RE sites for double digestion - (reply: 5)
771. Plasmid compatible with pBAD24 - (reply: 1)
772. I cant get colony - (reply: 3)
773. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
774. Vector with inducible/repressible system - (reply: 1)
775. cloning problem - (reply: 9)
776. Cloning genes into plant vector - (reply: 1)
777. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
778. Three-way ligation in two steps? - (reply: 3)
779. How does circular, uncut pGEM-T migrate? - (reply: 2)
780. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)