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Top : New Forum Archives (2009-): : Molecular-Cloning
751. problems with PCR confirmation of insert . HELP ! - (reply: 1)
752. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
753. Loss of promoter during ligation - (reply: 2)
754. Enzyme restriction - (reply: 8)
755. Colony PCR Problem!!!! - (reply: 5)
756. Removing unwanted sequence from pUC19 - (reply: 4)
757. transformation fails, bacteria grows in control plate - (reply: 6)
758. competentce cell, cloning - (reply: 2)
759. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
760. co-transformation - (reply: 3)
761. pcr clean-up fail? - (reply: 1)
762. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
763. sequential cloning of multiple PCR products - how to do? (reply: 3)
764. Cloning large gene - (reply: 3)
765. Glycosilated peptide's cDNA cloning - (reply: 3)
766. pWM91 cloning problem - (reply: 4)
767. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
768. Transformation - Transformation (reply: 1)
769. how to add 3XHA to the N-terminal of a protein - (reply: 5)
770. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
771. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
772. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
773. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
774. competent cells stock - (reply: 3)
775. Questions about pIRES-hrGFP1a - (reply: 1)
776. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
777. Cloning into lentiviral vector - (reply: 11)
778. cloning failure help plzzz - cloning (reply: 1)
779. use of pGEM -tT easy vectors - (reply: 2)
780. ~200kb Plasmid DNA purification is not working - (reply: 1)