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Top : New Forum Archives (2009-): : Molecular-Cloning
751. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
752. cDNA clone - (reply: 1)
753. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
754. why these guys use T7 instead of CMV? - for transfection (reply: 2)
755. Electroporation - no colonies (reply: 1)
756. enzymes inactivation - (reply: 3)
757. help: why plasmid yield dropped ever since the first culture - (reply: 2)
758. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
759. miRNA cloning - (reply: 2)
760. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
761. Single-digested vector shifts downward on gel - (reply: 1)
762. problem in digestion - (reply: 1)
763. Cloning does not work - (reply: 4)
764. Small Colonies After Transformation - (reply: 5)
765. Ligation and transformation problem - (reply: 2)
766. Agrobacterium EHA101 competent cells - (reply: 1)
767. restriction digestion against colony PCR - (reply: 1)
768. BAC for transgenic mice - (reply: 6)
769. Tag proteins for CO-IP and confocal - (reply: 1)
770. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
771. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
772. how long for a double digestion - (reply: 2)
773. Problems with Site-Directed Mutagenesis - (reply: 2)
774. blunt and sticky end clonning temperature - (reply: 2)
775. Most common mistakes - What mistakes are made during cloning? (reply: 7)
776. Luciferase Assay Stim. Concentration? - (reply: 1)
777. Cloning direction / orientation? - (reply: 2)
778. Question about plasmid midiprep - (reply: 1)
779. Does a polyA sequence need to be put in-frame? - (reply: 1)
780. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)