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751. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
752. What is the role of DMSO in yeast transformation - (reply: 1)
753. ligation problem - (reply: 3)
754. After Transfection - (reply: 1)
755. pGEMT easy TA cloning - does electroporation work? (reply: 1)
756. Help cloning large insert into large vector - (reply: 7)
757. problem about pIRES2-DsRed2 double digestion - (reply: 2)
758. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
759. hENT1 - (reply: 1)
760. digestion of insert twice and ligation of 3 bits - (reply: 6)
761. blunt end ligation problem - Self ligation and truncate (reply: 1)
762. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
763. neb ligase - (reply: 2)
764. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
765. problem about vector Dephosphorylation - (reply: 2)
766. Early transfection - (reply: 6)
767. Cloning: Randomly recombined Plasmids? - (reply: 6)
768. problem about fused protein - problem about fused protein (reply: 2)
769. Bacteria growing on plate but not liquid media - (reply: 8)
770. What means BamHI-linkered - (reply: 1)
771. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
772. Problems with Dpn1 - (reply: 5)
773. forgot to flip the plate over - but still got colonies (reply: 2)
774. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
775. Yet another ligation problem - (reply: 6)
776. Cloning problems - (reply: 7)
777. Bacteria and Amaxa - (reply: 2)
778. Problem in Ligation - (reply: 12)
779. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
780. multiway ligation - (reply: 6)
752. What is the role of DMSO in yeast transformation - (reply: 1)
753. ligation problem - (reply: 3)
754. After Transfection - (reply: 1)
755. pGEMT easy TA cloning - does electroporation work? (reply: 1)
756. Help cloning large insert into large vector - (reply: 7)
757. problem about pIRES2-DsRed2 double digestion - (reply: 2)
758. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
759. hENT1 - (reply: 1)
760. digestion of insert twice and ligation of 3 bits - (reply: 6)
761. blunt end ligation problem - Self ligation and truncate (reply: 1)
762. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
763. neb ligase - (reply: 2)
764. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
765. problem about vector Dephosphorylation - (reply: 2)
766. Early transfection - (reply: 6)
767. Cloning: Randomly recombined Plasmids? - (reply: 6)
768. problem about fused protein - problem about fused protein (reply: 2)
769. Bacteria growing on plate but not liquid media - (reply: 8)
770. What means BamHI-linkered - (reply: 1)
771. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
772. Problems with Dpn1 - (reply: 5)
773. forgot to flip the plate over - but still got colonies (reply: 2)
774. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
775. Yet another ligation problem - (reply: 6)
776. Cloning problems - (reply: 7)
777. Bacteria and Amaxa - (reply: 2)
778. Problem in Ligation - (reply: 12)
779. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
780. multiway ligation - (reply: 6)