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Top : New Forum Archives (2009-): : Molecular-Cloning
751. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
752. Cloning of IL8-promoter - (reply: 3)
753. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
754. Template DNA (plasmid) concentration for PCR - (reply: 4)
755. Ligation of 16kb PCR product - (reply: 5)
756. stable transfection - (reply: 1)
757. How to choose the right plasmids for certain types of cell? - (reply: 1)
758. Ligation needed after SDM with PfuX? - (reply: 3)
759. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
760. Cloning promoter (overlap with another gene!) - (reply: 3)
761. bacterial transfeomation - (reply: 2)
762. Loss of insert? - (reply: 1)
763. Sequencing trouble - (reply: 7)
764. Platinum-A cell trasnfection toruble - (reply: 1)
765. Cloning a promoter - (reply: 2)
766. pcDNA 3.1 His Expression - (reply: 2)
767. Cloning two fragments into a vector - (reply: 3)
768. confirmation of insert in to vector - (reply: 3)
769. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
770. Question about Molar ratio - (reply: 4)
771. Determining optimal digestion time for partial digestion - (reply: 2)
772. Ligation problem - (reply: 16)
773. double digestion with sac1, hindIII - (reply: 3)
774. self-ligation Anneled oligos - (reply: 7)
775. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
776. Looking for a GFP plasmid (non-amp) - (reply: 3)
777. clonning problem (recombined by-products) - (reply: 8)
778. Genecloning three types of molecules? - (reply: 11)
779. help in digesting DNA fragment using restriction enzyme - (reply: 2)
780. Tansformation fails ,how can i make it ? - (reply: 3)