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Top : New Forum Archives (2009-): : Molecular-Cloning
751. Dissolve IPTG in EtOH - (reply: 4)
752. problem with transient transfection - (reply: 6)
753. Recombinant construct looks smaller than empty vector - (reply: 3)
754. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
755. hygromycin concentration for e-coli selection - (reply: 1)
756. orientation of promoter into viral construct - (reply: 1)
757. problems with PCR confirmation of insert . HELP ! - (reply: 1)
758. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
759. Loss of promoter during ligation - (reply: 2)
760. Enzyme restriction - (reply: 8)
761. Colony PCR Problem!!!! - (reply: 5)
762. Removing unwanted sequence from pUC19 - (reply: 4)
763. transformation fails, bacteria grows in control plate - (reply: 6)
764. competentce cell, cloning - (reply: 2)
765. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
766. co-transformation - (reply: 3)
767. pcr clean-up fail? - (reply: 1)
768. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
769. sequential cloning of multiple PCR products - how to do? (reply: 3)
770. Cloning large gene - (reply: 3)
771. Glycosilated peptide's cDNA cloning - (reply: 3)
772. pWM91 cloning problem - (reply: 4)
773. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
774. Transformation - Transformation (reply: 1)
775. how to add 3XHA to the N-terminal of a protein - (reply: 5)
776. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
777. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
778. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
779. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
780. competent cells stock - (reply: 3)