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Top : New Forum Archives (2009-): : Molecular-Cloning
751. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
752. competent cells stock - (reply: 3)
753. Questions about pIRES-hrGFP1a - (reply: 1)
754. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
755. Cloning into lentiviral vector - (reply: 11)
756. cloning failure help plzzz - cloning (reply: 1)
757. use of pGEM -tT easy vectors - (reply: 2)
758. ~200kb Plasmid DNA purification is not working - (reply: 1)
759. ligating a 6 His tag to plasmid - (reply: 2)
760. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
761. ligation and transformation problem - (reply: 1)
762. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
763. Mini prep of frozen bacteria cells - (reply: 2)
764. PCR cloning problem - could not get colony (reply: 4)
765. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
766. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
767. PCR clonning - (reply: 3)
768. 2 bands in blue colony PCR - (reply: 5)
769. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
770. Introduction to vector development - Basics on vectors types and construction (reply: 1)
771. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
772. Targeted mutagenesis using a linear fragment - (reply: 1)
773. Transformation & Minimal Plates - (reply: 5)
774. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
775. Subcloning Problem - (reply: 3)
776. Restriction Enzyme sites in my genes - (reply: 1)
777. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
778. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
779. In-Fusion (Clontech) Reaction buffer - (reply: 1)
780. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)