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Top : New Forum Archives (2009-): : Molecular-Cloning
751. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
752. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
753. how long for a double digestion - (reply: 2)
754. Problems with Site-Directed Mutagenesis - (reply: 2)
755. blunt and sticky end clonning temperature - (reply: 2)
756. Most common mistakes - What mistakes are made during cloning? (reply: 7)
757. Luciferase Assay Stim. Concentration? - (reply: 1)
758. Cloning direction / orientation? - (reply: 2)
759. Question about plasmid midiprep - (reply: 1)
760. Does a polyA sequence need to be put in-frame? - (reply: 1)
761. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
762. Problem on growing up bugs - (reply: 8)
763. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
764. pSIM in BL21(DE3) - (reply: 2)
765. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
766. Weird problem in invitromutagenesis - (reply: 3)
767. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
768. RE digestion - (reply: 4)
769. HELP!!! - (reply: 3)
770. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
771. Cloning of ITS region - (reply: 6)
772. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
773. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
774. SmaI blunt end ligation problem... - (reply: 3)
775. AAV construct? - (reply: 1)
776. No bands on agarose gel after restriction digestion. - (reply: 10)
777. inverse pcr - (reply: 2)
778. Viral cDNA Library - (reply: 3)
779. metylated cloning vector - restriction digest of the insert (reply: 2)
780. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)