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Top : New Forum Archives (2009-): : Molecular-Cloning
751. stable transfection - (reply: 1)
752. How to choose the right plasmids for certain types of cell? - (reply: 1)
753. Ligation needed after SDM with PfuX? - (reply: 3)
754. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
755. Cloning promoter (overlap with another gene!) - (reply: 3)
756. bacterial transfeomation - (reply: 2)
757. Loss of insert? - (reply: 1)
758. Sequencing trouble - (reply: 7)
759. Platinum-A cell trasnfection toruble - (reply: 1)
760. Cloning a promoter - (reply: 2)
761. pcDNA 3.1 His Expression - (reply: 2)
762. Cloning two fragments into a vector - (reply: 3)
763. confirmation of insert in to vector - (reply: 3)
764. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
765. Question about Molar ratio - (reply: 4)
766. Determining optimal digestion time for partial digestion - (reply: 1)
767. Ligation problem - (reply: 16)
768. double digestion with sac1, hindIII - (reply: 3)
769. self-ligation Anneled oligos - (reply: 7)
770. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
771. Looking for a GFP plasmid (non-amp) - (reply: 3)
772. clonning problem (recombined by-products) - (reply: 8)
773. Genecloning three types of molecules? - (reply: 11)
774. help in digesting DNA fragment using restriction enzyme - (reply: 2)
775. Tansformation fails ,how can i make it ? - (reply: 3)
776. pelB leader - how to use - (reply: 1)
777. Transformation bacteria heat chock and incubation? - (reply: 2)
778. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
779. ligation of pcr product into expression vector - (reply: 4)
780. amp plates containing xgal/iptg with expression vector - (reply: 6)