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Top : New Forum Archives (2009-): : Molecular-Cloning
751. Template DNA (plasmid) concentration for PCR - (reply: 4)
752. Ligation of 16kb PCR product - (reply: 5)
753. stable transfection - (reply: 1)
754. How to choose the right plasmids for certain types of cell? - (reply: 1)
755. Ligation needed after SDM with PfuX? - (reply: 3)
756. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
757. Cloning promoter (overlap with another gene!) - (reply: 3)
758. bacterial transfeomation - (reply: 2)
759. Loss of insert? - (reply: 1)
760. Sequencing trouble - (reply: 7)
761. Platinum-A cell trasnfection toruble - (reply: 1)
762. Cloning a promoter - (reply: 2)
763. pcDNA 3.1 His Expression - (reply: 2)
764. Cloning two fragments into a vector - (reply: 3)
765. confirmation of insert in to vector - (reply: 3)
766. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
767. Question about Molar ratio - (reply: 4)
768. Determining optimal digestion time for partial digestion - (reply: 2)
769. Ligation problem - (reply: 16)
770. double digestion with sac1, hindIII - (reply: 3)
771. self-ligation Anneled oligos - (reply: 7)
772. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
773. Looking for a GFP plasmid (non-amp) - (reply: 3)
774. clonning problem (recombined by-products) - (reply: 8)
775. Genecloning three types of molecules? - (reply: 11)
776. help in digesting DNA fragment using restriction enzyme - (reply: 2)
777. Tansformation fails ,how can i make it ? - (reply: 3)
778. pelB leader - how to use - (reply: 1)
779. Transformation bacteria heat chock and incubation? - (reply: 2)
780. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)