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Top : New Forum Archives (2009-): : Molecular-Cloning
751. Ligation of double insert into pET31 failing - (reply: 1)
752. Homemade MiniPreps for Plasmid Purification - (reply: 1)
753. Agar has dried out during cultivation - (reply: 1)
754. Gateway cloning vector compatibility - (reply: 2)
755. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
756. Cloning of IL8-promoter - (reply: 3)
757. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
758. Template DNA (plasmid) concentration for PCR - (reply: 4)
759. Ligation of 16kb PCR product - (reply: 5)
760. stable transfection - (reply: 1)
761. How to choose the right plasmids for certain types of cell? - (reply: 1)
762. Ligation needed after SDM with PfuX? - (reply: 3)
763. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
764. Cloning promoter (overlap with another gene!) - (reply: 3)
765. bacterial transfeomation - (reply: 2)
766. Loss of insert? - (reply: 1)
767. Sequencing trouble - (reply: 7)
768. Platinum-A cell trasnfection toruble - (reply: 1)
769. Cloning a promoter - (reply: 2)
770. pcDNA 3.1 His Expression - (reply: 2)
771. Cloning two fragments into a vector - (reply: 3)
772. confirmation of insert in to vector - (reply: 3)
773. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
774. Question about Molar ratio - (reply: 4)
775. Determining optimal digestion time for partial digestion - (reply: 2)
776. Ligation problem - (reply: 16)
777. double digestion with sac1, hindIII - (reply: 3)
778. self-ligation Anneled oligos - (reply: 7)
779. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
780. Looking for a GFP plasmid (non-amp) - (reply: 3)