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Molecular-Cloning
1141.
Yet another ligation problem -
(reply: 6)
1142.
Cloning problems -
(reply: 7)
1143.
Bacteria and Amaxa -
(reply: 2)
1144.
Problem in Ligation -
(reply: 12)
1145.
Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem
(reply: 4)
1146.
multiway ligation -
(reply: 6)
1147.
Plasmid size problems! -
(reply: 3)
1148.
Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis
(reply: 3)
1149.
ligation problem -
(reply: 6)
1150.
Failed Ligation -
(reply: 2)
1151.
cloning of GroEL -
(reply: 3)
1152.
Ligation problem -
(reply: 9)
1153.
Problems with High Background with Gateway Cloning -
(reply: 1)
1154.
Mutagenesis troubles -
(reply: 2)
1155.
ligation/transformation failed -
(reply: 7)
1156.
Transfecting primary cells with plasmid -
(reply: 1)
1157.
Extra bands after QIAGEN spin miniprep -
(reply: 3)
1158.
T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG
(reply: 2)
1159.
The use of hygromycin -
(reply: 2)
1160.
QUESTION about cloning -
(reply: 5)
1161.
molecular cloning troubleshooting - bacterial sequences instead of my protein !
(reply: 3)
1162.
strategy of primer designing - difference between designing a primer for expression and cloning..
(reply: 1)
1163.
Ligation screening -
(reply: 4)
1164.
Double digestion and ligation problem -
(reply: 1)
1165.
competent cells - difference between electroporation and chemical comp. cells
(reply: 1)
1166.
Doubt with recombinant expression genes from streptobacillus moniliformis and Er -
(reply: 2)
1167.
Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio
(reply: 2)
1168.
CMV promoter -
(reply: 2)
1169.
insert in the reverse orientation after transformation -
(reply: 5)
1170.
cDNA as PCR template -
(reply: 1)
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