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Top : New Forum Archives (2009-): : Molecular-Cloning
1141. NICKED DNA - (reply: 2)
1142. cDNA clone - (reply: 1)
1143. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1144. Chosing the right competent cells - (reply: 1)
1145. Checking PCR insert (into pGEMT vector) - (reply: 2)
1146. problem with pGEMT - (reply: 3)
1147. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1148. Luciferase GL4 siRNA - (reply: 5)
1149. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1150. Culture Grow well but No Plasmid - (reply: 2)
1151. Insertion Mutation in Insert Sequence - (reply: 2)
1152. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1153. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1154. Protein expression with T7 promoter - (reply: 1)
1155. Triple ligation not working - (reply: 1)
1156. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1157. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1158. Problem with Induction (inclusion bodies?) - (reply: 4)
1159. Colony PCR screen positive - insert digestion negative - (reply: 2)
1160. plasmid purification kit for transfection - (reply: 2)
1161. Which pGL4 vector to choose - (reply: 2)
1162. Qiagen midi prepare low yield - (reply: 6)
1163. Help: incompatible RE site subcloning - (reply: 1)
1164. need help cloning cdna of a known protein - (reply: 1)
1165. restriction problem - (reply: 3)
1166. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1167. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1168. Trouble with subcloning - (reply: 6)
1169. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1170. Lb-kan plates life - How long can they be used? (reply: 2)