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1141. Yet another ligation problem - (reply: 6)
1142. Cloning problems - (reply: 7)
1143. Bacteria and Amaxa - (reply: 2)
1144. Problem in Ligation - (reply: 12)
1145. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1146. multiway ligation - (reply: 6)
1147. Plasmid size problems! - (reply: 3)
1148. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1149. ligation problem - (reply: 6)
1150. Failed Ligation - (reply: 2)
1151. cloning of GroEL - (reply: 3)
1152. Ligation problem - (reply: 9)
1153. Problems with High Background with Gateway Cloning - (reply: 1)
1154. Mutagenesis troubles - (reply: 2)
1155. ligation/transformation failed - (reply: 7)
1156. Transfecting primary cells with plasmid - (reply: 1)
1157. Extra bands after QIAGEN spin miniprep - (reply: 3)
1158. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1159. The use of hygromycin - (reply: 2)
1160. QUESTION about cloning - (reply: 5)
1161. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1162. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1163. Ligation screening - (reply: 4)
1164. Double digestion and ligation problem - (reply: 1)
1165. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1166. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1167. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1168. CMV promoter - (reply: 2)
1169. insert in the reverse orientation after transformation - (reply: 5)
1170. cDNA as PCR template - (reply: 1)
1142. Cloning problems - (reply: 7)
1143. Bacteria and Amaxa - (reply: 2)
1144. Problem in Ligation - (reply: 12)
1145. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1146. multiway ligation - (reply: 6)
1147. Plasmid size problems! - (reply: 3)
1148. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1149. ligation problem - (reply: 6)
1150. Failed Ligation - (reply: 2)
1151. cloning of GroEL - (reply: 3)
1152. Ligation problem - (reply: 9)
1153. Problems with High Background with Gateway Cloning - (reply: 1)
1154. Mutagenesis troubles - (reply: 2)
1155. ligation/transformation failed - (reply: 7)
1156. Transfecting primary cells with plasmid - (reply: 1)
1157. Extra bands after QIAGEN spin miniprep - (reply: 3)
1158. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1159. The use of hygromycin - (reply: 2)
1160. QUESTION about cloning - (reply: 5)
1161. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1162. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1163. Ligation screening - (reply: 4)
1164. Double digestion and ligation problem - (reply: 1)
1165. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1166. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1167. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1168. CMV promoter - (reply: 2)
1169. insert in the reverse orientation after transformation - (reply: 5)
1170. cDNA as PCR template - (reply: 1)