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1141. blunt end ligation problem - Self ligation and truncate (reply: 1)
1142. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1143. neb ligase - (reply: 2)
1144. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1145. problem about vector Dephosphorylation - (reply: 2)
1146. Early transfection - (reply: 6)
1147. Cloning: Randomly recombined Plasmids? - (reply: 6)
1148. problem about fused protein - problem about fused protein (reply: 2)
1149. Bacteria growing on plate but not liquid media - (reply: 8)
1150. What means BamHI-linkered - (reply: 1)
1151. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1152. Problems with Dpn1 - (reply: 5)
1153. forgot to flip the plate over - but still got colonies (reply: 2)
1154. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1155. Yet another ligation problem - (reply: 6)
1156. Cloning problems - (reply: 7)
1157. Bacteria and Amaxa - (reply: 2)
1158. Problem in Ligation - (reply: 12)
1159. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1160. multiway ligation - (reply: 6)
1161. Plasmid size problems! - (reply: 3)
1162. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1163. ligation problem - (reply: 6)
1164. Failed Ligation - (reply: 2)
1165. cloning of GroEL - (reply: 3)
1166. Ligation problem - (reply: 9)
1167. Problems with High Background with Gateway Cloning - (reply: 1)
1168. Mutagenesis troubles - (reply: 2)
1169. ligation/transformation failed - (reply: 7)
1170. Transfecting primary cells with plasmid - (reply: 1)
1142. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1143. neb ligase - (reply: 2)
1144. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1145. problem about vector Dephosphorylation - (reply: 2)
1146. Early transfection - (reply: 6)
1147. Cloning: Randomly recombined Plasmids? - (reply: 6)
1148. problem about fused protein - problem about fused protein (reply: 2)
1149. Bacteria growing on plate but not liquid media - (reply: 8)
1150. What means BamHI-linkered - (reply: 1)
1151. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1152. Problems with Dpn1 - (reply: 5)
1153. forgot to flip the plate over - but still got colonies (reply: 2)
1154. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1155. Yet another ligation problem - (reply: 6)
1156. Cloning problems - (reply: 7)
1157. Bacteria and Amaxa - (reply: 2)
1158. Problem in Ligation - (reply: 12)
1159. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1160. multiway ligation - (reply: 6)
1161. Plasmid size problems! - (reply: 3)
1162. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1163. ligation problem - (reply: 6)
1164. Failed Ligation - (reply: 2)
1165. cloning of GroEL - (reply: 3)
1166. Ligation problem - (reply: 9)
1167. Problems with High Background with Gateway Cloning - (reply: 1)
1168. Mutagenesis troubles - (reply: 2)
1169. ligation/transformation failed - (reply: 7)
1170. Transfecting primary cells with plasmid - (reply: 1)