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1141. Contruction of Expression Construct - (reply: 1)
1142. problem with subcloning, please help! - (reply: 8)
1143. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
1144. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
1145. Using DNA in gel extract kept at -20ºC - (reply: 2)
1146. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
1147. Help!!! - Unsure of problem (reply: 2)
1148. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
1149. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
1150. Site directed mutagenesis - Site directed mutagenesis (reply: 1)
1151. DNA yield estimation - (reply: 1)
1152. Vector NTI from Invitrogen - (reply: 4)
1153. gfp-tagged protein not expressing- help! - (reply: 3)
1154. anyone working currently on gateway cloning???? - (reply: 1)
1155. Dpn1 enzyme - (reply: 2)
1156. mutagenesis expert come in~ - (reply: 11)
1157. Subcloning - (reply: 3)
1158. Sequencing different colonies - (reply: 5)
1159. detection of restriction? - (reply: 1)
1160. problem in pcr and purification - (reply: 3)
1161. bacterial stock preparation - (reply: 3)
1162. Problem with site directed mutagenesis - (reply: 7)
1163. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1164. Forgot to add X-gal/IPTG - (reply: 8)
1165. after re-isolation of vector, get single band - (reply: 4)
1166. Subcloning into pMIR-REPORT-luc - (reply: 7)
1167. Restriction digestion troubleshooting - (reply: 10)
1168. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1169. Which digestion enzyme? - (reply: 1)
1170. Cloning big fragment into smaller vector - (reply: 3)
1142. problem with subcloning, please help! - (reply: 8)
1143. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
1144. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
1145. Using DNA in gel extract kept at -20ºC - (reply: 2)
1146. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
1147. Help!!! - Unsure of problem (reply: 2)
1148. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
1149. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
1150. Site directed mutagenesis - Site directed mutagenesis (reply: 1)
1151. DNA yield estimation - (reply: 1)
1152. Vector NTI from Invitrogen - (reply: 4)
1153. gfp-tagged protein not expressing- help! - (reply: 3)
1154. anyone working currently on gateway cloning???? - (reply: 1)
1155. Dpn1 enzyme - (reply: 2)
1156. mutagenesis expert come in~ - (reply: 11)
1157. Subcloning - (reply: 3)
1158. Sequencing different colonies - (reply: 5)
1159. detection of restriction? - (reply: 1)
1160. problem in pcr and purification - (reply: 3)
1161. bacterial stock preparation - (reply: 3)
1162. Problem with site directed mutagenesis - (reply: 7)
1163. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1164. Forgot to add X-gal/IPTG - (reply: 8)
1165. after re-isolation of vector, get single band - (reply: 4)
1166. Subcloning into pMIR-REPORT-luc - (reply: 7)
1167. Restriction digestion troubleshooting - (reply: 10)
1168. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1169. Which digestion enzyme? - (reply: 1)
1170. Cloning big fragment into smaller vector - (reply: 3)