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Top : New Forum Archives (2009-): : Molecular-Cloning
1141. Suicide vector - (reply: 2)
1142. Extremely small bacterial colonies after transformation - (reply: 10)
1143. tranformation efficiency - (reply: 2)
1144. transfection time - (reply: 2)
1145. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1146. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1147. Problem with SmaI restriction digest - (reply: 6)
1148. blunt-end / 3ŽA overhang cloning - (reply: 1)
1149. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1150. overexpression of stable clones - (reply: 1)
1151. functionality of yeast promoter in E. coli - (reply: 1)
1152. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1153. Can't seem to do a simple cloning - (reply: 2)
1154. Histogram from Nanodrop - (reply: 1)
1155. cDNA library construction - I get only one colony (reply: 2)
1156. Cloning into pIBV5-His (not working) - (reply: 1)
1157. Addition of Restriction sites into PCR primers - (reply: 4)
1158. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1159. Streaky DNA digest - (reply: 3)
1160. TOPO TA cloning problem - (reply: 12)
1161. cloning problem - (reply: 2)
1162. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1163. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1164. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1165. Nco1 digest problem - (reply: 4)
1166. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1167. ligation - (reply: 1)
1168. pre-prepararation of competent cells - (reply: 3)
1169. different copy # multimers with linker ligation? - (reply: 2)
1170. Help ADD 3 bases to DNA Sequences - (reply: 6)