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Top : New Forum Archives (2009-): : Molecular-Cloning
1141. Electrophoresis gel boxes and geldoc system - (reply: 2)
1142. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1143. problem with 293T cell co-transfection - (reply: 5)
1144. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1145. how CIP treatment is done - (reply: 3)
1146. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1147. Storage of gel extracted DNA before ligation - (reply: 3)
1148. Dual Luciferase Assay - (reply: 3)
1149. Ligation Problems - (reply: 2)
1150. NICKED DNA - (reply: 2)
1151. cDNA clone - (reply: 1)
1152. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1153. Chosing the right competent cells - (reply: 1)
1154. Checking PCR insert (into pGEMT vector) - (reply: 2)
1155. problem with pGEMT - (reply: 3)
1156. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1157. Luciferase GL4 siRNA - (reply: 5)
1158. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1159. Culture Grow well but No Plasmid - (reply: 2)
1160. Insertion Mutation in Insert Sequence - (reply: 2)
1161. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1162. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1163. Protein expression with T7 promoter - (reply: 1)
1164. Triple ligation not working - (reply: 1)
1165. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1166. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1167. Problem with Induction (inclusion bodies?) - (reply: 4)
1168. Colony PCR screen positive - insert digestion negative - (reply: 2)
1169. plasmid purification kit for transfection - (reply: 2)
1170. Which pGL4 vector to choose - (reply: 2)