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1141. After Transfection - (reply: 1)
1142. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1143. Help cloning large insert into large vector - (reply: 7)
1144. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1145. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1146. hENT1 - (reply: 1)
1147. digestion of insert twice and ligation of 3 bits - (reply: 6)
1148. blunt end ligation problem - Self ligation and truncate (reply: 1)
1149. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1150. neb ligase - (reply: 2)
1151. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1152. problem about vector Dephosphorylation - (reply: 2)
1153. Early transfection - (reply: 6)
1154. Cloning: Randomly recombined Plasmids? - (reply: 6)
1155. problem about fused protein - problem about fused protein (reply: 2)
1156. Bacteria growing on plate but not liquid media - (reply: 8)
1157. What means BamHI-linkered - (reply: 1)
1158. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1159. Problems with Dpn1 - (reply: 5)
1160. forgot to flip the plate over - but still got colonies (reply: 2)
1161. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1162. Yet another ligation problem - (reply: 6)
1163. Cloning problems - (reply: 7)
1164. Bacteria and Amaxa - (reply: 2)
1165. Problem in Ligation - (reply: 12)
1166. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1167. multiway ligation - (reply: 6)
1168. Plasmid size problems! - (reply: 3)
1169. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1170. ligation problem - (reply: 6)
1142. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1143. Help cloning large insert into large vector - (reply: 7)
1144. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1145. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1146. hENT1 - (reply: 1)
1147. digestion of insert twice and ligation of 3 bits - (reply: 6)
1148. blunt end ligation problem - Self ligation and truncate (reply: 1)
1149. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1150. neb ligase - (reply: 2)
1151. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1152. problem about vector Dephosphorylation - (reply: 2)
1153. Early transfection - (reply: 6)
1154. Cloning: Randomly recombined Plasmids? - (reply: 6)
1155. problem about fused protein - problem about fused protein (reply: 2)
1156. Bacteria growing on plate but not liquid media - (reply: 8)
1157. What means BamHI-linkered - (reply: 1)
1158. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1159. Problems with Dpn1 - (reply: 5)
1160. forgot to flip the plate over - but still got colonies (reply: 2)
1161. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1162. Yet another ligation problem - (reply: 6)
1163. Cloning problems - (reply: 7)
1164. Bacteria and Amaxa - (reply: 2)
1165. Problem in Ligation - (reply: 12)
1166. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1167. multiway ligation - (reply: 6)
1168. Plasmid size problems! - (reply: 3)
1169. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1170. ligation problem - (reply: 6)