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1141. COMPETENT CELL PREPARATION - (reply: 6)
1142. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1143. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1144. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1145. T A cloning problems - (reply: 3)
1146. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1147. Maximum recovery time after electroporation - (reply: 2)
1148. pCLBABE puro retriviral vector - (reply: 1)
1149. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1150. Blunt-end cloning problem - (reply: 6)
1151. Supercoiling blocking restriction site? - (reply: 6)
1152. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1153. problem in gene cloning - (reply: 4)
1154. Odd point mutations - (reply: 23)
1155. Plasmid linearization by PCR - (reply: 4)
1156. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1157. can't make the cloning work - (reply: 3)
1158. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1159. cloning help - (reply: 9)
1160. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1161. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1162. Ligation trouble continues - subcloning ligation failure (reply: 9)
1163. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1164. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1165. colony PCR - (reply: 8)
1166. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1167. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1168. Truncated sequence - (reply: 1)
1169. gateway cloning - (reply: 3)
1170. Purifying and cloning 69nt product - (reply: 5)
1142. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1143. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1144. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1145. T A cloning problems - (reply: 3)
1146. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1147. Maximum recovery time after electroporation - (reply: 2)
1148. pCLBABE puro retriviral vector - (reply: 1)
1149. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1150. Blunt-end cloning problem - (reply: 6)
1151. Supercoiling blocking restriction site? - (reply: 6)
1152. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1153. problem in gene cloning - (reply: 4)
1154. Odd point mutations - (reply: 23)
1155. Plasmid linearization by PCR - (reply: 4)
1156. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1157. can't make the cloning work - (reply: 3)
1158. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1159. cloning help - (reply: 9)
1160. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1161. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1162. Ligation trouble continues - subcloning ligation failure (reply: 9)
1163. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1164. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1165. colony PCR - (reply: 8)
1166. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1167. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1168. Truncated sequence - (reply: 1)
1169. gateway cloning - (reply: 3)
1170. Purifying and cloning 69nt product - (reply: 5)