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Top : New Forum Archives (2009-): : Molecular-Cloning
1141. Double digestion and ligation problem - (reply: 1)
1142. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1143. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1144. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1145. CMV promoter - (reply: 2)
1146. insert in the reverse orientation after transformation - (reply: 5)
1147. cDNA as PCR template - (reply: 1)
1148. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1149. T4 ligase buffer - (reply: 4)
1150. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1151. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1152. ligation problems - no colonies at the end!!!!! (reply: 4)
1153. Good stable mammalian promoter - (reply: 1)
1154. Self-ligation from a Double Digest - (reply: 4)
1155. Strange frameshift question - (reply: 2)
1156. Plasmid DNA mini-prep - no good isolation (reply: 5)
1157. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1158. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1159. BstUI digestion - (reply: 1)
1160. I have cloning problems with pGEM-T Easy - (reply: 5)
1161. CaCl2 for making competent cells - (reply: 3)
1162. best way to transport bacteria at room temperature - (reply: 5)
1163. Suicide vector - (reply: 2)
1164. Extremely small bacterial colonies after transformation - (reply: 10)
1165. tranformation efficiency - (reply: 2)
1166. transfection time - (reply: 2)
1167. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1168. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1169. Problem with SmaI restriction digest - (reply: 6)
1170. blunt-end / 3ŽA overhang cloning - (reply: 1)