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1141. Cloning PCR fragment - (reply: 1)
1142. molecular cloning - (reply: 2)
1143. DNA cloning - (reply: 4)
1144. Help with transformation - (reply: 3)
1145. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1146. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1147. Help cloning!!! - As usual cloning is not working! (reply: 11)
1148. pSuper cloning problems - (reply: 1)
1149. problem with plasmid extraction - (reply: 2)
1150. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1151. Smear in my restriction digest - (reply: 12)
1152. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1153. How to transfect two plasmids in a cell - (reply: 10)
1154. Double Digest using enzymes from diff companies - (reply: 4)
1155. Ligation problem (no colonies, but on negative control) - (reply: 3)
1156. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1157. SDM - (reply: 3)
1158. inframe problem - (reply: 1)
1159. Touchdown PCR - (reply: 1)
1160. Adding A tails? - (reply: 3)
1161. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1162. Determine annealing temperature of primers - (reply: 14)
1163. Bacteria pellet has almost-black region - (reply: 10)
1164. no ligation !!!!! - (reply: 6)
1165. How to clean up restriction enzyme digestion - (reply: 8)
1166. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1167. GPCR - (reply: 1)
1168. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1169. finding the suitable RE - first timer (reply: 6)
1170. TOPO TA Problems - TOPO TA Problems (reply: 8)
1142. molecular cloning - (reply: 2)
1143. DNA cloning - (reply: 4)
1144. Help with transformation - (reply: 3)
1145. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1146. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1147. Help cloning!!! - As usual cloning is not working! (reply: 11)
1148. pSuper cloning problems - (reply: 1)
1149. problem with plasmid extraction - (reply: 2)
1150. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1151. Smear in my restriction digest - (reply: 12)
1152. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1153. How to transfect two plasmids in a cell - (reply: 10)
1154. Double Digest using enzymes from diff companies - (reply: 4)
1155. Ligation problem (no colonies, but on negative control) - (reply: 3)
1156. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1157. SDM - (reply: 3)
1158. inframe problem - (reply: 1)
1159. Touchdown PCR - (reply: 1)
1160. Adding A tails? - (reply: 3)
1161. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1162. Determine annealing temperature of primers - (reply: 14)
1163. Bacteria pellet has almost-black region - (reply: 10)
1164. no ligation !!!!! - (reply: 6)
1165. How to clean up restriction enzyme digestion - (reply: 8)
1166. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1167. GPCR - (reply: 1)
1168. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1169. finding the suitable RE - first timer (reply: 6)
1170. TOPO TA Problems - TOPO TA Problems (reply: 8)