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1141. Mutagenesis troubles - (reply: 2)
1142. ligation/transformation failed - (reply: 7)
1143. Transfecting primary cells with plasmid - (reply: 1)
1144. Extra bands after QIAGEN spin miniprep - (reply: 3)
1145. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1146. The use of hygromycin - (reply: 2)
1147. QUESTION about cloning - (reply: 5)
1148. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1149. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1150. Ligation screening - (reply: 4)
1151. Double digestion and ligation problem - (reply: 1)
1152. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1153. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1154. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1155. CMV promoter - (reply: 2)
1156. insert in the reverse orientation after transformation - (reply: 5)
1157. cDNA as PCR template - (reply: 1)
1158. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1159. T4 ligase buffer - (reply: 4)
1160. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1161. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1162. ligation problems - no colonies at the end!!!!! (reply: 4)
1163. Good stable mammalian promoter - (reply: 1)
1164. Self-ligation from a Double Digest - (reply: 4)
1165. Strange frameshift question - (reply: 2)
1166. Plasmid DNA mini-prep - no good isolation (reply: 5)
1167. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1168. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1169. BstUI digestion - (reply: 1)
1170. I have cloning problems with pGEM-T Easy - (reply: 5)
1142. ligation/transformation failed - (reply: 7)
1143. Transfecting primary cells with plasmid - (reply: 1)
1144. Extra bands after QIAGEN spin miniprep - (reply: 3)
1145. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1146. The use of hygromycin - (reply: 2)
1147. QUESTION about cloning - (reply: 5)
1148. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1149. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1150. Ligation screening - (reply: 4)
1151. Double digestion and ligation problem - (reply: 1)
1152. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1153. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1154. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1155. CMV promoter - (reply: 2)
1156. insert in the reverse orientation after transformation - (reply: 5)
1157. cDNA as PCR template - (reply: 1)
1158. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1159. T4 ligase buffer - (reply: 4)
1160. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1161. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1162. ligation problems - no colonies at the end!!!!! (reply: 4)
1163. Good stable mammalian promoter - (reply: 1)
1164. Self-ligation from a Double Digest - (reply: 4)
1165. Strange frameshift question - (reply: 2)
1166. Plasmid DNA mini-prep - no good isolation (reply: 5)
1167. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1168. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1169. BstUI digestion - (reply: 1)
1170. I have cloning problems with pGEM-T Easy - (reply: 5)