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Top : New Forum Archives (2009-): : Molecular-Cloning
1141. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1142. ligation problem - (reply: 6)
1143. Failed Ligation - (reply: 2)
1144. cloning of GroEL - (reply: 3)
1145. Ligation problem - (reply: 9)
1146. Problems with High Background with Gateway Cloning - (reply: 1)
1147. Mutagenesis troubles - (reply: 2)
1148. ligation/transformation failed - (reply: 7)
1149. Transfecting primary cells with plasmid - (reply: 1)
1150. Extra bands after QIAGEN spin miniprep - (reply: 3)
1151. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1152. The use of hygromycin - (reply: 2)
1153. QUESTION about cloning - (reply: 5)
1154. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1155. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1156. Ligation screening - (reply: 4)
1157. Double digestion and ligation problem - (reply: 1)
1158. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1159. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1160. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1161. CMV promoter - (reply: 2)
1162. insert in the reverse orientation after transformation - (reply: 5)
1163. cDNA as PCR template - (reply: 1)
1164. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1165. T4 ligase buffer - (reply: 4)
1166. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1167. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1168. ligation problems - no colonies at the end!!!!! (reply: 4)
1169. Good stable mammalian promoter - (reply: 1)
1170. Self-ligation from a Double Digest - (reply: 4)