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Top : New Forum Archives (2009-): : Molecular-Cloning
1141. Transfecting primary cells with plasmid - (reply: 1)
1142. Extra bands after QIAGEN spin miniprep - (reply: 3)
1143. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1144. The use of hygromycin - (reply: 2)
1145. QUESTION about cloning - (reply: 5)
1146. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1147. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1148. Ligation screening - (reply: 4)
1149. Double digestion and ligation problem - (reply: 1)
1150. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1151. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1152. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1153. CMV promoter - (reply: 2)
1154. insert in the reverse orientation after transformation - (reply: 5)
1155. cDNA as PCR template - (reply: 1)
1156. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1157. T4 ligase buffer - (reply: 4)
1158. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1159. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1160. ligation problems - no colonies at the end!!!!! (reply: 4)
1161. Good stable mammalian promoter - (reply: 1)
1162. Self-ligation from a Double Digest - (reply: 4)
1163. Strange frameshift question - (reply: 2)
1164. Plasmid DNA mini-prep - no good isolation (reply: 5)
1165. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1166. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1167. BstUI digestion - (reply: 1)
1168. I have cloning problems with pGEM-T Easy - (reply: 5)
1169. CaCl2 for making competent cells - (reply: 3)
1170. best way to transport bacteria at room temperature - (reply: 5)