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1141. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1142. Problem with Induction (inclusion bodies?) - (reply: 4)
1143. Colony PCR screen positive - insert digestion negative - (reply: 2)
1144. plasmid purification kit for transfection - (reply: 2)
1145. Which pGL4 vector to choose - (reply: 2)
1146. Qiagen midi prepare low yield - (reply: 6)
1147. Help: incompatible RE site subcloning - (reply: 1)
1148. need help cloning cdna of a known protein - (reply: 1)
1149. restriction problem - (reply: 3)
1150. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1151. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1152. Trouble with subcloning - (reply: 6)
1153. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1154. Lb-kan plates life - How long can they be used? (reply: 2)
1155. LR reaction - Cloning into a expression vector (reply: 1)
1156. TOPO-Transformation Problem - (reply: 4)
1157. Checking Plasmid Expression - (reply: 1)
1158. designing primers for genes not sequenced yet - (reply: 2)
1159. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1160. Cloning Problem - (reply: 3)
1161. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1162. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1163. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1164. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1165. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1166. Restriction buffer composition - (reply: 2)
1167. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1168. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1169. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1170. Yet another ligation problem - (reply: 4)
1142. Problem with Induction (inclusion bodies?) - (reply: 4)
1143. Colony PCR screen positive - insert digestion negative - (reply: 2)
1144. plasmid purification kit for transfection - (reply: 2)
1145. Which pGL4 vector to choose - (reply: 2)
1146. Qiagen midi prepare low yield - (reply: 6)
1147. Help: incompatible RE site subcloning - (reply: 1)
1148. need help cloning cdna of a known protein - (reply: 1)
1149. restriction problem - (reply: 3)
1150. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1151. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1152. Trouble with subcloning - (reply: 6)
1153. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1154. Lb-kan plates life - How long can they be used? (reply: 2)
1155. LR reaction - Cloning into a expression vector (reply: 1)
1156. TOPO-Transformation Problem - (reply: 4)
1157. Checking Plasmid Expression - (reply: 1)
1158. designing primers for genes not sequenced yet - (reply: 2)
1159. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1160. Cloning Problem - (reply: 3)
1161. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1162. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1163. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1164. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1165. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1166. Restriction buffer composition - (reply: 2)
1167. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1168. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1169. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1170. Yet another ligation problem - (reply: 4)