Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
931. Cloning issue - (reply: 3)
932. Cohesive end filling after CIAP treatment - (reply: 2)
933. Looking for Pfu Taq for high GC-content template - (reply: 7)
934. questions about transformation - (reply: 4)
935. genotype lysogen DE3 - (reply: 1)
936. Need help! - Problem with right insertion! (reply: 1)
937. cloning a large insert - (reply: 5)
938. Transposase contamination in cloning - (reply: 7)
939. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
940. How long can I store cleaved/digested vector? - (reply: 9)
941. Cloning of a gene containing 31 exon - (reply: 1)
942. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
943. Optimum Molar ratio for 3-way ligation - (reply: 5)
944. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
945. cloning help - (reply: 3)
946. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
947. A gene is not inserted into the Plasmid - (reply: 2)
948. Air bubbles during electroporation - (reply: 1)
949. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
950. Colonies without inserts - (reply: 9)
951. Transformants Don't Grow - (reply: 4)
952. control DNA transformation- using pGEM kit - transformation (reply: 4)
953. Topo Cloning - Understanding the principle (reply: 6)
954. pMX-PURO Vector not growing - (reply: 3)
955. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
956. how to set up the ligation - (reply: 14)
957. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
958. DH5 Alpha Transformation - (reply: 2)
959. Cloning a foreign gene into E-coli - (reply: 1)
960. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)