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Top : New Forum Archives (2009-): : Molecular-Cloning
931. plasmid cloning - i need help.. can anyone answer me (reply: 5)
932. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
933. Topo cloning or pGEM-T easy ? - (reply: 3)
934. Problems with qiaquick gel purification kit - cloning (reply: 10)
935. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
936. integration of a gene per homologous recombination - (reply: 5)
937. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
938. Antarctic Phosphatase treatment - (reply: 5)
939. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
940. phage DNA - DNA wont migrate on agarose gel (reply: 3)
941. Plasmid out of BL21 for mutagensis - (reply: 3)
942. no colony growth - (reply: 4)
943. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
944. Effect of a DNA prep on DE3 - (reply: 2)
945. Competent cell storage for long term use - (reply: 2)
946. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
947. New to sequencing, primer design - (reply: 2)
948. ta cloning problem - (reply: 2)
949. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
950. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
951. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
952. Storage of Bacterial Culture at 4 degrees - (reply: 2)
953. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
954. rna isolation - (reply: 1)
955. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
956. Molecular Cloning od Recombinant DNA - (reply: 3)
957. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
958. looking for binding site - software? (reply: 1)
959. Insertion of loxp/avoiding SINE - (reply: 1)
960. Question: Ligation Problem.... - (reply: 2)