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Top : New Forum Archives (2009-): : Molecular-Cloning
931. Strange results of cloning - insert always reverse-oriented (reply: 2)
932. electroporation - (reply: 2)
933. transfection or infection - difference? (reply: 1)
934. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
935. No colonies after transformation! - (reply: 2)
936. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
937. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
938. Help Miniprep kit SIGMA - (reply: 2)
939. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
940. A question about pBR322 and pUC ori - (reply: 3)
941. Digestion of mini-prep by mistake - (reply: 3)
942. Cloning issue - (reply: 3)
943. Cohesive end filling after CIAP treatment - (reply: 2)
944. Looking for Pfu Taq for high GC-content template - (reply: 7)
945. questions about transformation - (reply: 4)
946. genotype lysogen DE3 - (reply: 1)
947. Need help! - Problem with right insertion! (reply: 1)
948. cloning a large insert - (reply: 5)
949. Transposase contamination in cloning - (reply: 7)
950. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
951. How long can I store cleaved/digested vector? - (reply: 9)
952. Cloning of a gene containing 31 exon - (reply: 1)
953. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
954. Optimum Molar ratio for 3-way ligation - (reply: 5)
955. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
956. cloning help - (reply: 3)
957. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
958. A gene is not inserted into the Plasmid - (reply: 2)
959. Air bubbles during electroporation - (reply: 1)
960. uncut plasmid dna vs linearized plasmid gel - (reply: 1)