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Top : New Forum Archives (2009-): : Molecular-Cloning
931. what is site-specific integration - (reply: 2)
932. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
933. double transformations - (reply: 1)
934. Unusual band in transformants - (reply: 2)
935. ligation--pls help! - (reply: 10)
936. bizarre digestion:) - (reply: 2)
937. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
938. metagenomic library consturction - BAC vectors (reply: 1)
939. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
940. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
941. Chromosomal recombination with BL21? - (reply: 2)
942. Restriction Digestion!! - (reply: 5)
943. digestion - restriction enzymes (reply: 2)
944. How to prevent self ligation - selfligation (reply: 11)
945. what is the difference between DH10B and Top10 - (reply: 1)
946. problems encountered when working on a large plasmid - (reply: 2)
947. make a Mega plasmid mutation - (reply: 1)
948. Cloning - (reply: 1)
949. Copy-number calculation formula? - (reply: 1)
950. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
951. low melt agarose - (reply: 1)
952. restriction digest - smear - (reply: 1)
953. designing of primers - (reply: 2)
954. Propagating ccdB survival cells - (reply: 6)
955. Bicistronic vector vs "normal" vector - (reply: 5)
956. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
957. Can't seem to get insert into vector... please help - (reply: 4)
958. plasmid cloning - i need help.. can anyone answer me (reply: 5)
959. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
960. Topo cloning or pGEM-T easy ? - (reply: 3)