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Top : New Forum Archives (2009-): : Molecular-Cloning
931. rna isolation - (reply: 1)
932. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
933. So many colonies! - (reply: 8)
934. Large vector cloning-recombination problem? - (reply: 1)
935. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
936. selecting recombinants - (reply: 1)
937. colony PCR for subcloning gene - (reply: 1)
938. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
939. cloning of a 20 kb insert - (reply: 2)
940. DNA quantification after gel purification - (reply: 6)
941. cloning of an operon - (reply: 2)
942. storing plasmid - -80degrees (reply: 4)
943. verifying a positive transformant - (reply: 3)
944. Need advice on cloning very small insert into a vector - (reply: 3)
945. Cloning problems i.e. Ligation - (reply: 2)
946. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
947. Radioactive carbon - (reply: 3)
948. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
949. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
950. Digesting large amount of plasmid - (reply: 4)
951. cleanup of digestion before ligation - (reply: 6)
952. transformation efficiency - (reply: 7)
953. transformed bacteria doesnt grow - (reply: 1)
954. TOPO Cloning - (reply: 5)
955. blunt ligation - (reply: 4)
956. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
957. About Laminin 5'UTR 's function - (reply: 1)
958. Vectors with special MCS - (reply: 5)
959. Cloning <100 bp fragments into a vector - (reply: 7)
960. Help! my plasmid size changed after ligation - (reply: 5)