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Top : New Forum Archives (2009-): : Molecular-Cloning
931. Cohesive end filling after CIAP treatment - (reply: 2)
932. Looking for Pfu Taq for high GC-content template - (reply: 7)
933. questions about transformation - (reply: 4)
934. genotype lysogen DE3 - (reply: 1)
935. Need help! - Problem with right insertion! (reply: 1)
936. cloning a large insert - (reply: 5)
937. Transposase contamination in cloning - (reply: 7)
938. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
939. How long can I store cleaved/digested vector? - (reply: 9)
940. Cloning of a gene containing 31 exon - (reply: 1)
941. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
942. Optimum Molar ratio for 3-way ligation - (reply: 5)
943. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
944. cloning help - (reply: 3)
945. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
946. A gene is not inserted into the Plasmid - (reply: 2)
947. Air bubbles during electroporation - (reply: 1)
948. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
949. Colonies without inserts - (reply: 9)
950. Transformants Don't Grow - (reply: 4)
951. control DNA transformation- using pGEM kit - transformation (reply: 4)
952. Topo Cloning - Understanding the principle (reply: 6)
953. pMX-PURO Vector not growing - (reply: 3)
954. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
955. how to set up the ligation - (reply: 14)
956. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
957. DH5 Alpha Transformation - (reply: 2)
958. Cloning a foreign gene into E-coli - (reply: 1)
959. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
960. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)