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Molecular-Cloning
931.
Restriction buffer composition -
(reply: 2)
932.
same weird weird staph transformation results - any idea what is going on?
(reply: 12)
933.
overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests?
(reply: 12)
934.
Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq?
(reply: 7)
935.
Yet another ligation problem -
(reply: 4)
936.
Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) -
(reply: 2)
937.
Promoter Cloning for Luciferase Assay -
(reply: 3)
938.
Creating mutants -
(reply: 3)
939.
cloning protocol -
(reply: 1)
940.
Ligation Time and Temp -
(reply: 4)
941.
Miniprep -
(reply: 3)
942.
PCR and then ligation -
(reply: 7)
943.
cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83
(reply: 1)
944.
3 way ligation help needed -
(reply: 10)
945.
Cloning and Expression Method Survey - Get some ideas from you all
(reply: 1)
946.
Cloning woes - Running out of ideas! -
(reply: 7)
947.
What fields of research other than microbiology work with bacteria and -
(reply: 1)
948.
How to inoculate bacteria? -
(reply: 10)
949.
What retrovirus vetor can have 7.5kb insert -
(reply: 1)
950.
need help in my Fse1 restriction ezyme -
(reply: 4)
951.
Insert problems with TA cloning -
(reply: 1)
952.
Surface expression by tansfection of CHO -
(reply: 3)
953.
please help!!! with transformation -
(reply: 10)
954.
sequencing primer: T7 or T7promoter? -
(reply: 4)
955.
Unstable clone? -
(reply: 1)
956.
Restriction enzymes -
(reply: 3)
957.
Problems facing in cloning with pBI121 vector -
(reply: 1)
958.
Failed Transformation of Tuner (DE3) cells -
(reply: 1)
959.
Is my insert present??? -
(reply: 17)
960.
Need help for restriction enzymes -
(reply: 5)
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