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Top : New Forum Archives (2009-): : Molecular-Cloning
931. electroporation - (reply: 2)
932. transfection or infection - difference? (reply: 1)
933. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
934. No colonies after transformation! - (reply: 2)
935. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
936. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
937. Help Miniprep kit SIGMA - (reply: 2)
938. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
939. A question about pBR322 and pUC ori - (reply: 3)
940. Digestion of mini-prep by mistake - (reply: 3)
941. Cloning issue - (reply: 3)
942. Cohesive end filling after CIAP treatment - (reply: 2)
943. Looking for Pfu Taq for high GC-content template - (reply: 7)
944. questions about transformation - (reply: 4)
945. genotype lysogen DE3 - (reply: 1)
946. Need help! - Problem with right insertion! (reply: 1)
947. cloning a large insert - (reply: 5)
948. Transposase contamination in cloning - (reply: 7)
949. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
950. How long can I store cleaved/digested vector? - (reply: 9)
951. Cloning of a gene containing 31 exon - (reply: 1)
952. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
953. Optimum Molar ratio for 3-way ligation - (reply: 5)
954. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
955. cloning help - (reply: 3)
956. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
957. A gene is not inserted into the Plasmid - (reply: 2)
958. Air bubbles during electroporation - (reply: 1)
959. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
960. Colonies without inserts - (reply: 9)