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Top : New Forum Archives (2009-): : Molecular-Cloning
931. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
932. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
933. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
934. which G418 concentration and treatment period should be used? - (reply: 2)
935. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
936. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
937. Chemical competent E.coli always from single colony ? - (reply: 2)
938. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
939. Strange results of cloning - insert always reverse-oriented (reply: 2)
940. electroporation - (reply: 2)
941. transfection or infection - difference? (reply: 1)
942. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
943. No colonies after transformation! - (reply: 2)
944. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
945. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
946. Help Miniprep kit SIGMA - (reply: 2)
947. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
948. A question about pBR322 and pUC ori - (reply: 3)
949. Digestion of mini-prep by mistake - (reply: 3)
950. Cloning issue - (reply: 3)
951. Cohesive end filling after CIAP treatment - (reply: 2)
952. Looking for Pfu Taq for high GC-content template - (reply: 7)
953. questions about transformation - (reply: 4)
954. genotype lysogen DE3 - (reply: 1)
955. Need help! - Problem with right insertion! (reply: 1)
956. cloning a large insert - (reply: 5)
957. Transposase contamination in cloning - (reply: 7)
958. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
959. How long can I store cleaved/digested vector? - (reply: 9)
960. Cloning of a gene containing 31 exon - (reply: 1)