Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
931. How long can I store cleaved/digested vector? - (reply: 9)
932. Cloning of a gene containing 31 exon - (reply: 1)
933. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
934. Optimum Molar ratio for 3-way ligation - (reply: 5)
935. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
936. cloning help - (reply: 3)
937. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
938. A gene is not inserted into the Plasmid - (reply: 2)
939. Air bubbles during electroporation - (reply: 1)
940. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
941. Colonies without inserts - (reply: 9)
942. Transformants Don't Grow - (reply: 4)
943. control DNA transformation- using pGEM kit - transformation (reply: 4)
944. Topo Cloning - Understanding the principle (reply: 6)
945. pMX-PURO Vector not growing - (reply: 3)
946. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
947. how to set up the ligation - (reply: 14)
948. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
949. DH5 Alpha Transformation - (reply: 2)
950. Cloning a foreign gene into E-coli - (reply: 1)
951. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
952. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
953. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
954. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
955. restriction digest simple question - (reply: 4)
956. ligating small inert into a large vector - (reply: 4)
957. tranformation trouble using dh5alpha - (reply: 1)
958. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
959. Starting cells for making one's own competent cells - (reply: 7)
960. 6 months... and still no positive clones! - (reply: 4)