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Top : New Forum Archives (2009-): : Molecular-Cloning
931. 6 months... and still no positive clones! - (reply: 4)
932. Really strange problem with sequencing and plasmid purification! - (reply: 8)
933. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
934. what is site-specific integration - (reply: 2)
935. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
936. double transformations - (reply: 1)
937. Unusual band in transformants - (reply: 2)
938. ligation--pls help! - (reply: 10)
939. bizarre digestion:) - (reply: 2)
940. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
941. metagenomic library consturction - BAC vectors (reply: 1)
942. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
943. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
944. Chromosomal recombination with BL21? - (reply: 2)
945. Restriction Digestion!! - (reply: 5)
946. digestion - restriction enzymes (reply: 2)
947. How to prevent self ligation - selfligation (reply: 11)
948. what is the difference between DH10B and Top10 - (reply: 1)
949. problems encountered when working on a large plasmid - (reply: 2)
950. make a Mega plasmid mutation - (reply: 1)
951. Cloning - (reply: 1)
952. Copy-number calculation formula? - (reply: 1)
953. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
954. low melt agarose - (reply: 1)
955. restriction digest - smear - (reply: 1)
956. designing of primers - (reply: 2)
957. Propagating ccdB survival cells - (reply: 6)
958. Bicistronic vector vs "normal" vector - (reply: 5)
959. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
960. Can't seem to get insert into vector... please help - (reply: 4)