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Top : New Forum Archives (2009-): : Molecular-Cloning
931. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
932. How long can I store cleaved/digested vector? - (reply: 9)
933. Cloning of a gene containing 31 exon - (reply: 1)
934. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
935. Optimum Molar ratio for 3-way ligation - (reply: 5)
936. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
937. cloning help - (reply: 3)
938. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
939. A gene is not inserted into the Plasmid - (reply: 2)
940. Air bubbles during electroporation - (reply: 1)
941. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
942. Colonies without inserts - (reply: 9)
943. Transformants Don't Grow - (reply: 4)
944. control DNA transformation- using pGEM kit - transformation (reply: 4)
945. Topo Cloning - Understanding the principle (reply: 6)
946. pMX-PURO Vector not growing - (reply: 3)
947. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
948. how to set up the ligation - (reply: 14)
949. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
950. DH5 Alpha Transformation - (reply: 2)
951. Cloning a foreign gene into E-coli - (reply: 1)
952. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
953. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
954. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
955. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
956. restriction digest simple question - (reply: 4)
957. ligating small inert into a large vector - (reply: 4)
958. tranformation trouble using dh5alpha - (reply: 1)
959. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
960. Starting cells for making one's own competent cells - (reply: 7)