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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Oligo insert cloning - (reply: 3)
422. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
423. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
424. How to select competent E. coli cells? - (reply: 7)
425. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
426. cDNA amplification problem - (reply: 4)
427. Ligation: two basic questions - (reply: 1)
428. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
429. Unexpected base after deletion mutagenesis - (reply: 3)
430. Confirmation of ligation - (reply: 7)
431. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
432. Ligation of large ammount of insert (no transformation) - (reply: 7)
433. PCR and restriction enzyme digestion - (reply: 3)
434. Probelm with double digestion - (reply: 1)
435. Cloning not working, all steps checked, clueless - (reply: 1)
436. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
437. recombinant protein form - (reply: 2)
438. restricted PCR plasmid runs slower - (reply: 2)
439. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
440. High frequency of vector religation - (reply: 10)
441. Cloned but not expressing! - (reply: 8)
442. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
443. DNA ligation and trasformation - (reply: 6)
444. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
445. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
446. How to set up simultaneous digestion? - (reply: 3)
447. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
448. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
449. Freeze transformation mixture? - (reply: 2)
450. Cutting my plasmid made it larger - (reply: 7)