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Top : New Forum Archives (2009-): : Molecular-Cloning
421. problem in cloning PCR primer design with restriction site - (reply: 4)
422. quick change mutagenesis...... - (reply: 1)
423. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
424. Help for pGEM-T easy vector ligation problem - (reply: 2)
425. Co-transformation - (reply: 2)
426. Problems regarding point mutations.... - (reply: 4)
427. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
428. pkp 59 vector - (reply: 2)
429. Vector insert ratio in cloning - (reply: 3)
430. polymerase to use for cloning - (reply: 4)
431. Oligo insert cloning - (reply: 3)
432. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
433. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
434. How to select competent E. coli cells? - (reply: 7)
435. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
436. cDNA amplification problem - (reply: 4)
437. Ligation: two basic questions - (reply: 1)
438. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
439. Unexpected base after deletion mutagenesis - (reply: 3)
440. Confirmation of ligation - (reply: 7)
441. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
442. Ligation of large ammount of insert (no transformation) - (reply: 7)
443. PCR and restriction enzyme digestion - (reply: 3)
444. Probelm with double digestion - (reply: 1)
445. Cloning not working, all steps checked, clueless - (reply: 1)
446. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
447. recombinant protein form - (reply: 2)
448. restricted PCR plasmid runs slower - (reply: 2)
449. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
450. High frequency of vector religation - (reply: 10)