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Top : New Forum Archives (2009-): : Molecular-Cloning
421. pkp 59 vector - (reply: 2)
422. Vector insert ratio in cloning - (reply: 3)
423. polymerase to use for cloning - (reply: 4)
424. Oligo insert cloning - (reply: 3)
425. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
426. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
427. How to select competent E. coli cells? - (reply: 7)
428. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
429. cDNA amplification problem - (reply: 4)
430. Ligation: two basic questions - (reply: 1)
431. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
432. Unexpected base after deletion mutagenesis - (reply: 3)
433. Confirmation of ligation - (reply: 7)
434. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
435. Ligation of large ammount of insert (no transformation) - (reply: 7)
436. PCR and restriction enzyme digestion - (reply: 3)
437. Probelm with double digestion - (reply: 1)
438. Cloning not working, all steps checked, clueless - (reply: 1)
439. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
440. recombinant protein form - (reply: 2)
441. restricted PCR plasmid runs slower - (reply: 2)
442. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
443. High frequency of vector religation - (reply: 10)
444. Cloned but not expressing! - (reply: 8)
445. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
446. DNA ligation and trasformation - (reply: 6)
447. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
448. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
449. How to set up simultaneous digestion? - (reply: 3)
450. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)