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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Issue with Xba1 and Dam methylation - (reply: 7)
422. Restriction site rearrangement - (reply: 1)
423. Mutation in cloning - (reply: 6)
424. Must the terminator be in frame? - (reply: 1)
425. sequencing problems - (reply: 2)
426. Understand the basics of molecular cloning - (reply: 6)
427. Maintainence of transformed cells - (reply: 5)
428. Restriction digest band shift? - (reply: 1)
429. problem in cloning PCR primer design with restriction site - (reply: 4)
430. quick change mutagenesis...... - (reply: 1)
431. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
432. Help for pGEM-T easy vector ligation problem - (reply: 2)
433. Co-transformation - (reply: 2)
434. Problems regarding point mutations.... - (reply: 4)
435. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
436. pkp 59 vector - (reply: 2)
437. Vector insert ratio in cloning - (reply: 3)
438. polymerase to use for cloning - (reply: 4)
439. Oligo insert cloning - (reply: 3)
440. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
441. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
442. How to select competent E. coli cells? - (reply: 7)
443. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
444. cDNA amplification problem - (reply: 4)
445. Ligation: two basic questions - (reply: 1)
446. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
447. Unexpected base after deletion mutagenesis - (reply: 3)
448. Confirmation of ligation - (reply: 7)
449. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
450. Ligation of large ammount of insert (no transformation) - (reply: 7)