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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Co-transformation - (reply: 2)
422. Problems regarding point mutations.... - (reply: 4)
423. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
424. pkp 59 vector - (reply: 2)
425. Vector insert ratio in cloning - (reply: 3)
426. polymerase to use for cloning - (reply: 4)
427. Oligo insert cloning - (reply: 3)
428. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
429. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
430. How to select competent E. coli cells? - (reply: 7)
431. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
432. cDNA amplification problem - (reply: 4)
433. Ligation: two basic questions - (reply: 1)
434. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
435. Unexpected base after deletion mutagenesis - (reply: 3)
436. Confirmation of ligation - (reply: 7)
437. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
438. Ligation of large ammount of insert (no transformation) - (reply: 7)
439. PCR and restriction enzyme digestion - (reply: 3)
440. Probelm with double digestion - (reply: 1)
441. Cloning not working, all steps checked, clueless - (reply: 1)
442. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
443. recombinant protein form - (reply: 2)
444. restricted PCR plasmid runs slower - (reply: 2)
445. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
446. High frequency of vector religation - (reply: 10)
447. Cloned but not expressing! - (reply: 8)
448. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
449. DNA ligation and trasformation - (reply: 6)
450. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)