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Top : New Forum Archives (2009-): : Molecular-Cloning
421. cloning to pET28a - (reply: 1)
422. Weird Library Transformation Results - (reply: 10)
423. Injecting mice with lentivirus - (reply: 1)
424. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
425. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
426. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
427. Ligation into a BAC? - (reply: 2)
428. oligo continually appears less on gel compared to other same load - (reply: 2)
429. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
430. plasmid tetR and tet promoter - (reply: 2)
431. unable to amplify an infectious retrovirus clone - (reply: 4)
432. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
433. No bands at all after double digestion - (reply: 1)
434. Digest for insert orientation - (reply: 4)
435. Colonies from transformation plate are not growing...:( - (reply: 5)
436. Problem with ligating a small insert (LoxP site) - (reply: 5)
437. Is the genomic DNA similar among different cell lines? - (reply: 4)
438. molecular cloning - (reply: 3)
439. Swich selection in plasmid - (reply: 3)
440. Sub-cloning and transformation - (reply: 17)
441. cloning without phosphorylation - (reply: 7)
442. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
443. A good vector to make gene library? - (reply: 5)
444. problems with midiprep - (reply: 3)
445. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
446. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
447. 900bp insertion into PIRESnEO,problem - (reply: 2)
448. fusion of cDNA expressing protein with gfp - (reply: 1)
449. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
450. Problem with plasmids transformation - (reply: 6)