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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Site-directed, PCR works but no colonies - (reply: 2)
422. Simoltanious Multi-restriction digestion - (reply: 3)
423. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
424. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
425. Restriction digestion problems - (reply: 3)
426. should I do blunt end my pcr product before ligation - (reply: 2)
427. Maxiprep of freeze/thawed pellet. - (reply: 1)
428. Digest with AarI and Pml I - (reply: 1)
429. Help with CGL gene cloning problems - (reply: 1)
430. screening for GPF - (reply: 10)
431. Creating a pGEM-T easy + insert sequence map - (reply: 1)
432. where to get vector overexpressing caveolin-1? - (reply: 2)
433. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
434. pINDUCER series - (reply: 1)
435. LZRS vector details - (reply: 1)
436. 12.4kb vector 6.7kb insert - (reply: 2)
437. Stable transfection - (reply: 2)
438. Using normal competent cells in electroporation method? - (reply: 1)
439. an effective way to do a yeast colony pcr - (reply: 2)
440. pGEM-T Easy vector contamination - (reply: 2)
441. recover old transformed DH5 - (reply: 1)
442. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
443. oligo(dt) 15 vs random primers - (reply: 3)
444. I need bynary vector pART27 - (reply: 1)
445. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
446. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
447. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
448. cloning into Pgem t easy - (reply: 1)
449. How to get pure protein of a gene - (reply: 3)
450. How to insert genes into chromosome of E. coli - (reply: 1)