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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Vector insert ratio in cloning - (reply: 3)
422. polymerase to use for cloning - (reply: 4)
423. Oligo insert cloning - (reply: 3)
424. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
425. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
426. How to select competent E. coli cells? - (reply: 7)
427. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
428. cDNA amplification problem - (reply: 4)
429. Ligation: two basic questions - (reply: 1)
430. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
431. Unexpected base after deletion mutagenesis - (reply: 3)
432. Confirmation of ligation - (reply: 7)
433. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
434. Ligation of large ammount of insert (no transformation) - (reply: 7)
435. PCR and restriction enzyme digestion - (reply: 3)
436. Probelm with double digestion - (reply: 1)
437. Cloning not working, all steps checked, clueless - (reply: 1)
438. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
439. recombinant protein form - (reply: 2)
440. restricted PCR plasmid runs slower - (reply: 2)
441. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
442. High frequency of vector religation - (reply: 10)
443. Cloned but not expressing! - (reply: 8)
444. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
445. DNA ligation and trasformation - (reply: 6)
446. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
447. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
448. How to set up simultaneous digestion? - (reply: 3)
449. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
450. Problem with ligation using pJET and pGEM for clone library - (reply: 11)