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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Enzyme cutting: low efficiency - (reply: 5)
422. Issue with Xba1 and Dam methylation - (reply: 7)
423. Restriction site rearrangement - (reply: 1)
424. Mutation in cloning - (reply: 6)
425. Must the terminator be in frame? - (reply: 1)
426. sequencing problems - (reply: 2)
427. Understand the basics of molecular cloning - (reply: 6)
428. Maintainence of transformed cells - (reply: 5)
429. Restriction digest band shift? - (reply: 1)
430. problem in cloning PCR primer design with restriction site - (reply: 4)
431. quick change mutagenesis...... - (reply: 1)
432. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
433. Help for pGEM-T easy vector ligation problem - (reply: 2)
434. Co-transformation - (reply: 2)
435. Problems regarding point mutations.... - (reply: 4)
436. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
437. pkp 59 vector - (reply: 2)
438. Vector insert ratio in cloning - (reply: 3)
439. polymerase to use for cloning - (reply: 4)
440. Oligo insert cloning - (reply: 3)
441. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
442. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
443. How to select competent E. coli cells? - (reply: 7)
444. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
445. cDNA amplification problem - (reply: 4)
446. Ligation: two basic questions - (reply: 1)
447. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
448. Unexpected base after deletion mutagenesis - (reply: 3)
449. Confirmation of ligation - (reply: 7)
450. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)