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421. transformation fails, bacteria grows in control plate - (reply: 6)
422. competentce cell, cloning - (reply: 2)
423. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
424. co-transformation - (reply: 3)
425. pcr clean-up fail? - (reply: 1)
426. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
427. sequential cloning of multiple PCR products - how to do? (reply: 3)
428. Cloning large gene - (reply: 3)
429. Glycosilated peptide's cDNA cloning - (reply: 3)
430. pWM91 cloning problem - (reply: 4)
431. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
432. Transformation - Transformation (reply: 1)
433. how to add 3XHA to the N-terminal of a protein - (reply: 5)
434. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
435. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
436. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
437. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
438. competent cells stock - (reply: 3)
439. Questions about pIRES-hrGFP1a - (reply: 1)
440. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
441. Cloning into lentiviral vector - (reply: 11)
442. cloning failure help plzzz - cloning (reply: 1)
443. use of pGEM -tT easy vectors - (reply: 2)
444. ~200kb Plasmid DNA purification is not working - (reply: 1)
445. ligating a 6 His tag to plasmid - (reply: 2)
446. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
447. ligation and transformation problem - (reply: 1)
448. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
449. Mini prep of frozen bacteria cells - (reply: 2)
450. PCR cloning problem - could not get colony (reply: 4)
422. competentce cell, cloning - (reply: 2)
423. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
424. co-transformation - (reply: 3)
425. pcr clean-up fail? - (reply: 1)
426. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
427. sequential cloning of multiple PCR products - how to do? (reply: 3)
428. Cloning large gene - (reply: 3)
429. Glycosilated peptide's cDNA cloning - (reply: 3)
430. pWM91 cloning problem - (reply: 4)
431. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
432. Transformation - Transformation (reply: 1)
433. how to add 3XHA to the N-terminal of a protein - (reply: 5)
434. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
435. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
436. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
437. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
438. competent cells stock - (reply: 3)
439. Questions about pIRES-hrGFP1a - (reply: 1)
440. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
441. Cloning into lentiviral vector - (reply: 11)
442. cloning failure help plzzz - cloning (reply: 1)
443. use of pGEM -tT easy vectors - (reply: 2)
444. ~200kb Plasmid DNA purification is not working - (reply: 1)
445. ligating a 6 His tag to plasmid - (reply: 2)
446. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
447. ligation and transformation problem - (reply: 1)
448. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
449. Mini prep of frozen bacteria cells - (reply: 2)
450. PCR cloning problem - could not get colony (reply: 4)