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Top : New Forum Archives (2009-): : Molecular-Cloning
421. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
422. Large quantity heat shock trouble? - (reply: 3)
423. molecular cloning - (reply: 7)
424. Cannot see insert with colony pcr - (reply: 6)
425. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
426. help with a vector - (reply: 3)
427. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
428. Another cloning hell....no transformants - (reply: 1)
429. Agrobacterium LBA4404 transformation - (reply: 1)
430. TOPO TA Cloning Troubleshooting - (reply: 1)
431. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
432. Tricistronic vector - (reply: 1)
433. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
434. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
435. Directions of genes expression illustrations - (reply: 5)
436. Plasmid DNA at RT for a few days - (reply: 4)
437. TOPO TA Cloning - (reply: 1)
438. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
439. No insert in subcloning - (reply: 3)
440. Digest does not confirm presence of fragment - (reply: 5)
441. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
442. pGL3 Promoter replacing TSS bases - (reply: 1)
443. lipofectamine 2000:DNA complex stability - (reply: 2)
444. Problems with Ecoli glycerol stock - (reply: 6)
445. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
446. CIAP dilution - (reply: 5)
447. Problems in RNAi construction by using pUC19 - (reply: 3)
448. Unable to clone in E. coli cells - (reply: 3)
449. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
450. plasmid rearrangement or sabotage - (reply: 2)