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Top : New Forum Archives (2009-): : Molecular-Cloning
421. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
422. Help for pGEM-T easy vector ligation problem - (reply: 2)
423. Co-transformation - (reply: 2)
424. Problems regarding point mutations.... - (reply: 4)
425. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
426. pkp 59 vector - (reply: 2)
427. Vector insert ratio in cloning - (reply: 3)
428. polymerase to use for cloning - (reply: 4)
429. Oligo insert cloning - (reply: 3)
430. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
431. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
432. How to select competent E. coli cells? - (reply: 7)
433. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
434. cDNA amplification problem - (reply: 4)
435. Ligation: two basic questions - (reply: 1)
436. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
437. Unexpected base after deletion mutagenesis - (reply: 3)
438. Confirmation of ligation - (reply: 7)
439. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
440. Ligation of large ammount of insert (no transformation) - (reply: 7)
441. PCR and restriction enzyme digestion - (reply: 3)
442. Probelm with double digestion - (reply: 1)
443. Cloning not working, all steps checked, clueless - (reply: 1)
444. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
445. recombinant protein form - (reply: 2)
446. restricted PCR plasmid runs slower - (reply: 2)
447. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
448. High frequency of vector religation - (reply: 10)
449. Cloned but not expressing! - (reply: 8)
450. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)