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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Restriction digest band shift? - (reply: 1)
422. problem in cloning PCR primer design with restriction site - (reply: 4)
423. quick change mutagenesis...... - (reply: 1)
424. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
425. Help for pGEM-T easy vector ligation problem - (reply: 2)
426. Co-transformation - (reply: 2)
427. Problems regarding point mutations.... - (reply: 4)
428. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
429. pkp 59 vector - (reply: 2)
430. Vector insert ratio in cloning - (reply: 3)
431. polymerase to use for cloning - (reply: 4)
432. Oligo insert cloning - (reply: 3)
433. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
434. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
435. How to select competent E. coli cells? - (reply: 7)
436. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
437. cDNA amplification problem - (reply: 4)
438. Ligation: two basic questions - (reply: 1)
439. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
440. Unexpected base after deletion mutagenesis - (reply: 3)
441. Confirmation of ligation - (reply: 7)
442. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
443. Ligation of large ammount of insert (no transformation) - (reply: 7)
444. PCR and restriction enzyme digestion - (reply: 3)
445. Probelm with double digestion - (reply: 1)
446. Cloning not working, all steps checked, clueless - (reply: 1)
447. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
448. recombinant protein form - (reply: 2)
449. restricted PCR plasmid runs slower - (reply: 2)
450. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)