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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Ligation troubleshooting! Please help! - (reply: 5)
422. Truncated gene Cloning - (reply: 1)
423. Screening for ligation and transformation result - (reply: 2)
424. Problem with 3 way ligation - (reply: 2)
425. Query regarding primers for quick change mutagenesis - (reply: 3)
426. What is NEB's CutSmart Buffer? - (reply: 5)
427. Enzyme cutting: low efficiency - (reply: 5)
428. Issue with Xba1 and Dam methylation - (reply: 7)
429. Restriction site rearrangement - (reply: 1)
430. Mutation in cloning - (reply: 6)
431. Must the terminator be in frame? - (reply: 1)
432. sequencing problems - (reply: 2)
433. Understand the basics of molecular cloning - (reply: 6)
434. Maintainence of transformed cells - (reply: 5)
435. Restriction digest band shift? - (reply: 1)
436. problem in cloning PCR primer design with restriction site - (reply: 4)
437. quick change mutagenesis...... - (reply: 1)
438. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
439. Help for pGEM-T easy vector ligation problem - (reply: 2)
440. Co-transformation - (reply: 2)
441. Problems regarding point mutations.... - (reply: 4)
442. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
443. pkp 59 vector - (reply: 2)
444. Vector insert ratio in cloning - (reply: 3)
445. polymerase to use for cloning - (reply: 4)
446. Oligo insert cloning - (reply: 3)
447. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
448. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
449. How to select competent E. coli cells? - (reply: 7)
450. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)