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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Unexpected base after deletion mutagenesis - (reply: 3)
422. Confirmation of ligation - (reply: 7)
423. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
424. Ligation of large ammount of insert (no transformation) - (reply: 7)
425. PCR and restriction enzyme digestion - (reply: 3)
426. Probelm with double digestion - (reply: 1)
427. Cloning not working, all steps checked, clueless - (reply: 1)
428. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
429. recombinant protein form - (reply: 2)
430. restricted PCR plasmid runs slower - (reply: 2)
431. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
432. High frequency of vector religation - (reply: 10)
433. Cloned but not expressing! - (reply: 8)
434. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
435. DNA ligation and trasformation - (reply: 6)
436. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
437. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
438. How to set up simultaneous digestion? - (reply: 3)
439. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
440. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
441. Freeze transformation mixture? - (reply: 2)
442. Cutting my plasmid made it larger - (reply: 7)
443. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
444. Amplification of CAG promoter problems - (reply: 4)
445. restriction enzyme - (reply: 2)
446. Recreate original plasmid by cutting out insert - (reply: 2)
447. Colony-PCR workaround - (reply: 3)
448. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
449. Can I pause a bacterial culture at 4C - (reply: 2)
450. TOPO TA CLONING - LIGATION STEP - (reply: 9)