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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Unable to clone in E. coli cells - (reply: 3)
422. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
423. plasmid rearrangement or sabotage - (reply: 2)
424. pLDR20 Vector - (reply: 1)
425. surprising behavior of transformants needs immediate advice! - (reply: 8)
426. cloning antisense promoter in pGL3Basic - (reply: 2)
427. PhiX174 DNA - (reply: 1)
428. Competent bacterial cells - (reply: 2)
429. Replacing puramycin in a pLKO1.puro - (reply: 1)
430. colony cracking for screening of positive clones - (reply: 2)
431. deletion of large fregments - (reply: 3)
432. Linearising plasmid before transfection? - (reply: 2)
433. Smear in plasmid sample - (reply: 9)
434. about a book - (reply: 1)
435. Cloning Problem! I need help - (reply: 3)
436. Help designing primers for use in In-fushion cloning - (reply: 4)
437. Repeat of desired mutation in cloning product - (reply: 1)
438. vector dephosphorylation - (reply: 5)
439. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
440. DH10BAC - (reply: 3)
441. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
442. protein is not expessing in HEK 293 cells - (reply: 2)
443. Restriction problems - (reply: 4)
444. cloning a gene in to a plasmid - (reply: 5)
445. no product on gel after restriction digestion - (reply: 3)
446. Gateway technology. - (reply: 4)
447. Primer concentration for cDNA synthesis using GSP - (reply: 1)
448. cloning from cDNA but got a much shorter PCR product - (reply: 4)
449. GeneArt vs Quikchange - (reply: 1)
450. why do some colonies keep growing in the chiller? - (reply: 7)