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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Alternative to pET Vectorsystem - (reply: 2)
422. pEGFP C-1/N-1 Cloning - (reply: 11)
423. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
424. Problems with growing out transformants in liquid LB - (reply: 3)
425. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
426. Ethidiumbromide - (reply: 3)
427. TRYPSIN - (reply: 4)
428. Problem with cloning - what is wrong? - (reply: 6)
429. cloning: band at different site than desired after RE digestion - (reply: 11)
430. GST TAG - (reply: 3)
431. Residues on the N terminal GST Tag - (reply: 1)
432. Where to place the polyA signal? - (reply: 1)
433. identification of target sequence - (reply: 3)
434. cloning pcr insert into plasmid - (reply: 2)
435. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
436. Large quantity heat shock trouble? - (reply: 3)
437. molecular cloning - (reply: 7)
438. Cannot see insert with colony pcr - (reply: 6)
439. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
440. help with a vector - (reply: 3)
441. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
442. Another cloning hell....no transformants - (reply: 1)
443. Agrobacterium LBA4404 transformation - (reply: 1)
444. TOPO TA Cloning Troubleshooting - (reply: 1)
445. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
446. Tricistronic vector - (reply: 1)
447. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
448. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
449. Directions of genes expression illustrations - (reply: 5)
450. Plasmid DNA at RT for a few days - (reply: 4)