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Top : New Forum Archives (2009-): : Molecular-Cloning
421. polymerase to use for cloning - (reply: 4)
422. Oligo insert cloning - (reply: 3)
423. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
424. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
425. How to select competent E. coli cells? - (reply: 7)
426. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
427. cDNA amplification problem - (reply: 4)
428. Ligation: two basic questions - (reply: 1)
429. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
430. Unexpected base after deletion mutagenesis - (reply: 3)
431. Confirmation of ligation - (reply: 7)
432. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
433. Ligation of large ammount of insert (no transformation) - (reply: 7)
434. PCR and restriction enzyme digestion - (reply: 3)
435. Probelm with double digestion - (reply: 1)
436. Cloning not working, all steps checked, clueless - (reply: 1)
437. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
438. recombinant protein form - (reply: 2)
439. restricted PCR plasmid runs slower - (reply: 2)
440. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
441. High frequency of vector religation - (reply: 10)
442. Cloned but not expressing! - (reply: 8)
443. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
444. DNA ligation and trasformation - (reply: 6)
445. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
446. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
447. How to set up simultaneous digestion? - (reply: 3)
448. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
449. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
450. Freeze transformation mixture? - (reply: 2)