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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Problem with 3 way ligation - (reply: 2)
422. Query regarding primers for quick change mutagenesis - (reply: 3)
423. What is NEB's CutSmart Buffer? - (reply: 5)
424. Enzyme cutting: low efficiency - (reply: 5)
425. Issue with Xba1 and Dam methylation - (reply: 7)
426. Restriction site rearrangement - (reply: 1)
427. Mutation in cloning - (reply: 6)
428. Must the terminator be in frame? - (reply: 1)
429. sequencing problems - (reply: 2)
430. Understand the basics of molecular cloning - (reply: 6)
431. Maintainence of transformed cells - (reply: 5)
432. Restriction digest band shift? - (reply: 1)
433. problem in cloning PCR primer design with restriction site - (reply: 4)
434. quick change mutagenesis...... - (reply: 1)
435. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
436. Help for pGEM-T easy vector ligation problem - (reply: 2)
437. Co-transformation - (reply: 2)
438. Problems regarding point mutations.... - (reply: 4)
439. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
440. pkp 59 vector - (reply: 2)
441. Vector insert ratio in cloning - (reply: 3)
442. polymerase to use for cloning - (reply: 4)
443. Oligo insert cloning - (reply: 3)
444. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
445. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
446. How to select competent E. coli cells? - (reply: 7)
447. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
448. cDNA amplification problem - (reply: 4)
449. Ligation: two basic questions - (reply: 1)
450. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)