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Top : New Forum Archives (2009-): : Molecular-Cloning
421. Is ligation really necessary? - (reply: 6)
422. problem with ligation of linker/adaptor - (reply: 2)
423. GFP -weak signal -why? - (reply: 1)
424. Ligation troubleshooting! Please help! - (reply: 5)
425. Truncated gene Cloning - (reply: 1)
426. Screening for ligation and transformation result - (reply: 2)
427. Problem with 3 way ligation - (reply: 2)
428. Query regarding primers for quick change mutagenesis - (reply: 3)
429. What is NEB's CutSmart Buffer? - (reply: 5)
430. Enzyme cutting: low efficiency - (reply: 5)
431. Issue with Xba1 and Dam methylation - (reply: 7)
432. Restriction site rearrangement - (reply: 1)
433. Mutation in cloning - (reply: 6)
434. Must the terminator be in frame? - (reply: 1)
435. sequencing problems - (reply: 2)
436. Understand the basics of molecular cloning - (reply: 6)
437. Maintainence of transformed cells - (reply: 5)
438. Restriction digest band shift? - (reply: 1)
439. problem in cloning PCR primer design with restriction site - (reply: 4)
440. quick change mutagenesis...... - (reply: 1)
441. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
442. Help for pGEM-T easy vector ligation problem - (reply: 2)
443. Co-transformation - (reply: 2)
444. Problems regarding point mutations.... - (reply: 4)
445. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
446. pkp 59 vector - (reply: 2)
447. Vector insert ratio in cloning - (reply: 3)
448. polymerase to use for cloning - (reply: 4)
449. Oligo insert cloning - (reply: 3)
450. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)