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Top : New Forum Archives (2009-): : Molecular-Cloning
421. cloning from cDNA but got a much shorter PCR product - (reply: 4)
422. GeneArt vs Quikchange - (reply: 1)
423. why do some colonies keep growing in the chiller? - (reply: 7)
424. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
425. Difference between types of bacterial culture broths? - (reply: 4)
426. Co-transfection with 2 plasmids - (reply: 10)
427. Low passage HEK293?? - (reply: 3)
428. Vector Digestion Problem - (reply: 4)
429. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
430. Transformation with a big plasmid - (reply: 4)
431. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
432. Three way ligation problem - (reply: 6)
433. problems with recovery of dna from gel and ligation - (reply: 5)
434. Colony PCR and digestion with KpnI - (reply: 10)
435. Greenish tinge to bacterial culture - (reply: 3)
436. SURE E. coli competent cells - (reply: 1)
437. Ligation - (reply: 20)
438. E. coli growing on plates and bot not in LB medium - (reply: 10)
439. *** Human Insulin promoter sequence*** - (reply: 2)
440. digested plasmid - (reply: 2)
441. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
442. Subcloning - RE digest and ligation and transformation - (reply: 5)
443. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
444. Making DNA Linkers for cloning purposes - (reply: 1)
445. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
446. Dam-/+ e. coli strains - (reply: 6)
447. cloning short genomic DNA fragments - (reply: 1)
448. finding the protein and nucleotide matches - (reply: 1)
449. DH10B with big construct question - (reply: 2)
450. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 14)