Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
421. Agrobacterium LBA4404 transformation - (reply: 1)
422. TOPO TA Cloning Troubleshooting - (reply: 1)
423. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
424. Tricistronic vector - (reply: 1)
425. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
426. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
427. Directions of genes expression illustrations - (reply: 5)
428. Plasmid DNA at RT for a few days - (reply: 4)
429. TOPO TA Cloning - (reply: 1)
430. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
431. No insert in subcloning - (reply: 3)
432. Digest does not confirm presence of fragment - (reply: 5)
433. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
434. pGL3 Promoter replacing TSS bases - (reply: 1)
435. lipofectamine 2000:DNA complex stability - (reply: 2)
436. Problems with Ecoli glycerol stock - (reply: 6)
437. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
438. CIAP dilution - (reply: 5)
439. Problems in RNAi construction by using pUC19 - (reply: 3)
440. Unable to clone in E. coli cells - (reply: 3)
441. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
442. plasmid rearrangement or sabotage - (reply: 2)
443. pLDR20 Vector - (reply: 1)
444. surprising behavior of transformants needs immediate advice! - (reply: 8)
445. cloning antisense promoter in pGL3Basic - (reply: 2)
446. PhiX174 DNA - (reply: 1)
447. Competent bacterial cells - (reply: 2)
448. Replacing puramycin in a pLKO1.puro - (reply: 1)
449. colony cracking for screening of positive clones - (reply: 2)
450. deletion of large fregments - (reply: 3)