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Top : New Forum Archives (2009-): : Molecular-Cloning
421. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
422. Incomplete digestion of plasmid with single enzyme - (reply: 1)
423. problem with plasmid digestion - (reply: 5)
424. Difficult Ligation - Details Inside - (reply: 9)
425. pET 32 (+) - (reply: 1)
426. Alternative to pET Vectorsystem - (reply: 2)
427. pEGFP C-1/N-1 Cloning - (reply: 11)
428. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
429. Problems with growing out transformants in liquid LB - (reply: 3)
430. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
431. Ethidiumbromide - (reply: 3)
432. TRYPSIN - (reply: 4)
433. Problem with cloning - what is wrong? - (reply: 6)
434. cloning: band at different site than desired after RE digestion - (reply: 11)
435. GST TAG - (reply: 3)
436. Residues on the N terminal GST Tag - (reply: 1)
437. Where to place the polyA signal? - (reply: 1)
438. identification of target sequence - (reply: 3)
439. cloning pcr insert into plasmid - (reply: 2)
440. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
441. Large quantity heat shock trouble? - (reply: 3)
442. molecular cloning - (reply: 7)
443. Cannot see insert with colony pcr - (reply: 6)
444. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
445. help with a vector - (reply: 3)
446. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
447. Another cloning hell....no transformants - (reply: 1)
448. Agrobacterium LBA4404 transformation - (reply: 1)
449. TOPO TA Cloning Troubleshooting - (reply: 1)
450. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)