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Top : New Forum Archives (2009-): : Molecular-Cloning
421. quick change mutagenesis...... - (reply: 1)
422. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
423. Help for pGEM-T easy vector ligation problem - (reply: 2)
424. Co-transformation - (reply: 2)
425. Problems regarding point mutations.... - (reply: 4)
426. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
427. pkp 59 vector - (reply: 2)
428. Vector insert ratio in cloning - (reply: 3)
429. polymerase to use for cloning - (reply: 4)
430. Oligo insert cloning - (reply: 3)
431. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
432. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
433. How to select competent E. coli cells? - (reply: 7)
434. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
435. cDNA amplification problem - (reply: 4)
436. Ligation: two basic questions - (reply: 1)
437. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
438. Unexpected base after deletion mutagenesis - (reply: 3)
439. Confirmation of ligation - (reply: 7)
440. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
441. Ligation of large ammount of insert (no transformation) - (reply: 7)
442. PCR and restriction enzyme digestion - (reply: 3)
443. Probelm with double digestion - (reply: 1)
444. Cloning not working, all steps checked, clueless - (reply: 1)
445. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
446. recombinant protein form - (reply: 2)
447. restricted PCR plasmid runs slower - (reply: 2)
448. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
449. High frequency of vector religation - (reply: 10)
450. Cloned but not expressing! - (reply: 8)