Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
421. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
422. protein is not expessing in HEK 293 cells - (reply: 2)
423. Restriction problems - (reply: 4)
424. cloning a gene in to a plasmid - (reply: 5)
425. no product on gel after restriction digestion - (reply: 3)
426. Gateway technology. - (reply: 4)
427. Primer concentration for cDNA synthesis using GSP - (reply: 1)
428. cloning from cDNA but got a much shorter PCR product - (reply: 4)
429. GeneArt vs Quikchange - (reply: 1)
430. why do some colonies keep growing in the chiller? - (reply: 7)
431. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
432. Difference between types of bacterial culture broths? - (reply: 4)
433. Co-transfection with 2 plasmids - (reply: 10)
434. Low passage HEK293?? - (reply: 3)
435. Vector Digestion Problem - (reply: 4)
436. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
437. Transformation with a big plasmid - (reply: 4)
438. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
439. Three way ligation problem - (reply: 6)
440. problems with recovery of dna from gel and ligation - (reply: 5)
441. Colony PCR and digestion with KpnI - (reply: 10)
442. Greenish tinge to bacterial culture - (reply: 3)
443. SURE E. coli competent cells - (reply: 1)
444. Ligation - (reply: 20)
445. E. coli growing on plates and bot not in LB medium - (reply: 10)
446. *** Human Insulin promoter sequence*** - (reply: 2)
447. digested plasmid - (reply: 2)
448. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
449. Subcloning - RE digest and ligation and transformation - (reply: 5)
450. Plasmid digested with RE used to transform...what will happen? - (reply: 14)