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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Incomplete digestion of plasmid with single enzyme - (reply: 1)
482. problem with plasmid digestion - (reply: 5)
483. Difficult Ligation - Details Inside - (reply: 9)
484. pET 32 (+) - (reply: 1)
485. Alternative to pET Vectorsystem - (reply: 2)
486. pEGFP C-1/N-1 Cloning - (reply: 11)
487. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
488. Problems with growing out transformants in liquid LB - (reply: 3)
489. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
490. Ethidiumbromide - (reply: 3)
491. TRYPSIN - (reply: 4)
492. Problem with cloning - what is wrong? - (reply: 6)
493. cloning: band at different site than desired after RE digestion - (reply: 11)
494. GST TAG - (reply: 3)
495. Residues on the N terminal GST Tag - (reply: 1)
496. Where to place the polyA signal? - (reply: 1)
497. identification of target sequence - (reply: 3)
498. cloning pcr insert into plasmid - (reply: 2)
499. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
500. Large quantity heat shock trouble? - (reply: 3)
501. molecular cloning - (reply: 7)
502. Cannot see insert with colony pcr - (reply: 6)
503. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
504. help with a vector - (reply: 3)
505. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
506. Another cloning hell....no transformants - (reply: 1)
507. Agrobacterium LBA4404 transformation - (reply: 1)
508. TOPO TA Cloning Troubleshooting - (reply: 1)
509. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
510. Tricistronic vector - (reply: 1)