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Top : New Forum Archives (2009-): : Molecular-Cloning
481. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
482. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
483. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
484. Ligation into a BAC? - (reply: 2)
485. oligo continually appears less on gel compared to other same load - (reply: 2)
486. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
487. plasmid tetR and tet promoter - (reply: 2)
488. unable to amplify an infectious retrovirus clone - (reply: 4)
489. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
490. No bands at all after double digestion - (reply: 1)
491. Digest for insert orientation - (reply: 4)
492. Colonies from transformation plate are not growing...:( - (reply: 5)
493. Problem with ligating a small insert (LoxP site) - (reply: 5)
494. Is the genomic DNA similar among different cell lines? - (reply: 4)
495. molecular cloning - (reply: 3)
496. Swich selection in plasmid - (reply: 3)
497. Sub-cloning and transformation - (reply: 17)
498. cloning without phosphorylation - (reply: 7)
499. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
500. A good vector to make gene library? - (reply: 5)
501. problems with midiprep - (reply: 3)
502. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
503. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
504. 900bp insertion into PIRESnEO,problem - (reply: 2)
505. fusion of cDNA expressing protein with gfp - (reply: 1)
506. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
507. Problem with plasmids transformation - (reply: 6)
508. Cloning genetic sequences of proteins. - (reply: 1)
509. problem of restriction enzyme digestion - (reply: 3)
510. Site-directed, PCR works but no colonies - (reply: 2)