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Top : New Forum Archives (2009-): : Molecular-Cloning
481. TRYPSIN - (reply: 4)
482. Problem with cloning - what is wrong? - (reply: 6)
483. cloning: band at different site than desired after RE digestion - (reply: 11)
484. GST TAG - (reply: 3)
485. Residues on the N terminal GST Tag - (reply: 1)
486. Where to place the polyA signal? - (reply: 1)
487. identification of target sequence - (reply: 3)
488. cloning pcr insert into plasmid - (reply: 2)
489. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
490. Large quantity heat shock trouble? - (reply: 3)
491. molecular cloning - (reply: 7)
492. Cannot see insert with colony pcr - (reply: 6)
493. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
494. help with a vector - (reply: 3)
495. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
496. Another cloning hell....no transformants - (reply: 1)
497. Agrobacterium LBA4404 transformation - (reply: 1)
498. TOPO TA Cloning Troubleshooting - (reply: 1)
499. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
500. Tricistronic vector - (reply: 1)
501. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
502. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
503. Directions of genes expression illustrations - (reply: 5)
504. Plasmid DNA at RT for a few days - (reply: 4)
505. TOPO TA Cloning - (reply: 1)
506. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
507. No insert in subcloning - (reply: 3)
508. Digest does not confirm presence of fragment - (reply: 5)
509. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
510. pGL3 Promoter replacing TSS bases - (reply: 1)