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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Low passage HEK293?? - (reply: 3)
482. Vector Digestion Problem - (reply: 4)
483. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
484. Transformation with a big plasmid - (reply: 4)
485. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
486. Three way ligation problem - (reply: 6)
487. problems with recovery of dna from gel and ligation - (reply: 5)
488. Colony PCR and digestion with KpnI - (reply: 10)
489. Greenish tinge to bacterial culture - (reply: 3)
490. SURE E. coli competent cells - (reply: 1)
491. Ligation - (reply: 20)
492. E. coli growing on plates and bot not in LB medium - (reply: 10)
493. *** Human Insulin promoter sequence*** - (reply: 2)
494. digested plasmid - (reply: 2)
495. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
496. Subcloning - RE digest and ligation and transformation - (reply: 5)
497. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
498. Making DNA Linkers for cloning purposes - (reply: 1)
499. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
500. Dam-/+ e. coli strains - (reply: 6)
501. cloning short genomic DNA fragments - (reply: 1)
502. finding the protein and nucleotide matches - (reply: 1)
503. DH10B with big construct question - (reply: 2)
504. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
505. cloning to pET28a - (reply: 1)
506. Weird Library Transformation Results - (reply: 10)
507. Injecting mice with lentivirus - (reply: 1)
508. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
509. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
510. mamallian expression vector that has FLAG tag and GFP - (reply: 3)