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Top : New Forum Archives (2009-): : Molecular-Cloning
481. pEGFP C-1/N-1 Cloning - (reply: 11)
482. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
483. Problems with growing out transformants in liquid LB - (reply: 3)
484. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
485. Ethidiumbromide - (reply: 3)
486. TRYPSIN - (reply: 4)
487. Problem with cloning - what is wrong? - (reply: 6)
488. cloning: band at different site than desired after RE digestion - (reply: 11)
489. GST TAG - (reply: 3)
490. Residues on the N terminal GST Tag - (reply: 1)
491. Where to place the polyA signal? - (reply: 1)
492. identification of target sequence - (reply: 3)
493. cloning pcr insert into plasmid - (reply: 2)
494. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
495. Large quantity heat shock trouble? - (reply: 3)
496. molecular cloning - (reply: 7)
497. Cannot see insert with colony pcr - (reply: 6)
498. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
499. help with a vector - (reply: 3)
500. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
501. Another cloning hell....no transformants - (reply: 1)
502. Agrobacterium LBA4404 transformation - (reply: 1)
503. TOPO TA Cloning Troubleshooting - (reply: 1)
504. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
505. Tricistronic vector - (reply: 1)
506. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
507. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
508. Directions of genes expression illustrations - (reply: 5)
509. Plasmid DNA at RT for a few days - (reply: 4)
510. TOPO TA Cloning - (reply: 1)