|
481. enzymes inactivation - (reply: 3)
482. help: why plasmid yield dropped ever since the first culture - (reply: 2)
483. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
484. miRNA cloning - (reply: 2)
485. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
486. Single-digested vector shifts downward on gel - (reply: 1)
487. problem in digestion - (reply: 1)
488. Cloning does not work - (reply: 4)
489. Small Colonies After Transformation - (reply: 5)
490. Ligation and transformation problem - (reply: 2)
491. Agrobacterium EHA101 competent cells - (reply: 1)
492. restriction digestion against colony PCR - (reply: 1)
493. BAC for transgenic mice - (reply: 6)
494. Tag proteins for CO-IP and confocal - (reply: 1)
495. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
496. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
497. how long for a double digestion - (reply: 2)
498. Problems with Site-Directed Mutagenesis - (reply: 2)
499. blunt and sticky end clonning temperature - (reply: 2)
500. Most common mistakes - What mistakes are made during cloning? (reply: 7)
501. Luciferase Assay Stim. Concentration? - (reply: 1)
502. Cloning direction / orientation? - (reply: 2)
503. Question about plasmid midiprep - (reply: 1)
504. Does a polyA sequence need to be put in-frame? - (reply: 1)
505. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
506. Problem on growing up bugs - (reply: 8)
507. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
508. pSIM in BL21(DE3) - (reply: 2)
509. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
510. Weird problem in invitromutagenesis - (reply: 3)
482. help: why plasmid yield dropped ever since the first culture - (reply: 2)
483. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
484. miRNA cloning - (reply: 2)
485. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
486. Single-digested vector shifts downward on gel - (reply: 1)
487. problem in digestion - (reply: 1)
488. Cloning does not work - (reply: 4)
489. Small Colonies After Transformation - (reply: 5)
490. Ligation and transformation problem - (reply: 2)
491. Agrobacterium EHA101 competent cells - (reply: 1)
492. restriction digestion against colony PCR - (reply: 1)
493. BAC for transgenic mice - (reply: 6)
494. Tag proteins for CO-IP and confocal - (reply: 1)
495. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
496. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
497. how long for a double digestion - (reply: 2)
498. Problems with Site-Directed Mutagenesis - (reply: 2)
499. blunt and sticky end clonning temperature - (reply: 2)
500. Most common mistakes - What mistakes are made during cloning? (reply: 7)
501. Luciferase Assay Stim. Concentration? - (reply: 1)
502. Cloning direction / orientation? - (reply: 2)
503. Question about plasmid midiprep - (reply: 1)
504. Does a polyA sequence need to be put in-frame? - (reply: 1)
505. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
506. Problem on growing up bugs - (reply: 8)
507. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
508. pSIM in BL21(DE3) - (reply: 2)
509. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
510. Weird problem in invitromutagenesis - (reply: 3)