Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
481. Problems with growing out transformants in liquid LB - (reply: 3)
482. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
483. Ethidiumbromide - (reply: 3)
484. TRYPSIN - (reply: 4)
485. Problem with cloning - what is wrong? - (reply: 6)
486. cloning: band at different site than desired after RE digestion - (reply: 11)
487. GST TAG - (reply: 3)
488. Residues on the N terminal GST Tag - (reply: 1)
489. Where to place the polyA signal? - (reply: 1)
490. identification of target sequence - (reply: 3)
491. cloning pcr insert into plasmid - (reply: 2)
492. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
493. Large quantity heat shock trouble? - (reply: 3)
494. molecular cloning - (reply: 7)
495. Cannot see insert with colony pcr - (reply: 6)
496. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
497. help with a vector - (reply: 3)
498. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
499. Another cloning hell....no transformants - (reply: 1)
500. Agrobacterium LBA4404 transformation - (reply: 1)
501. TOPO TA Cloning Troubleshooting - (reply: 1)
502. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
503. Tricistronic vector - (reply: 1)
504. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
505. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
506. Directions of genes expression illustrations - (reply: 5)
507. Plasmid DNA at RT for a few days - (reply: 4)
508. TOPO TA Cloning - (reply: 1)
509. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
510. No insert in subcloning - (reply: 3)