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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
482. Ethidiumbromide - (reply: 3)
483. TRYPSIN - (reply: 4)
484. Problem with cloning - what is wrong? - (reply: 6)
485. cloning: band at different site than desired after RE digestion - (reply: 11)
486. GST TAG - (reply: 3)
487. Residues on the N terminal GST Tag - (reply: 1)
488. Where to place the polyA signal? - (reply: 1)
489. identification of target sequence - (reply: 3)
490. cloning pcr insert into plasmid - (reply: 2)
491. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
492. Large quantity heat shock trouble? - (reply: 3)
493. molecular cloning - (reply: 7)
494. Cannot see insert with colony pcr - (reply: 6)
495. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
496. help with a vector - (reply: 3)
497. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
498. Another cloning hell....no transformants - (reply: 1)
499. Agrobacterium LBA4404 transformation - (reply: 1)
500. TOPO TA Cloning Troubleshooting - (reply: 1)
501. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
502. Tricistronic vector - (reply: 1)
503. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
504. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
505. Directions of genes expression illustrations - (reply: 5)
506. Plasmid DNA at RT for a few days - (reply: 4)
507. TOPO TA Cloning - (reply: 1)
508. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
509. No insert in subcloning - (reply: 3)
510. Digest does not confirm presence of fragment - (reply: 5)