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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Directions of genes expression illustrations - (reply: 5)
482. Plasmid DNA at RT for a few days - (reply: 4)
483. TOPO TA Cloning - (reply: 1)
484. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
485. No insert in subcloning - (reply: 3)
486. Digest does not confirm presence of fragment - (reply: 5)
487. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
488. pGL3 Promoter replacing TSS bases - (reply: 1)
489. lipofectamine 2000:DNA complex stability - (reply: 2)
490. Problems with Ecoli glycerol stock - (reply: 6)
491. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
492. CIAP dilution - (reply: 5)
493. Problems in RNAi construction by using pUC19 - (reply: 3)
494. Unable to clone in E. coli cells - (reply: 3)
495. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
496. plasmid rearrangement or sabotage - (reply: 2)
497. pLDR20 Vector - (reply: 1)
498. surprising behavior of transformants needs immediate advice! - (reply: 8)
499. cloning antisense promoter in pGL3Basic - (reply: 2)
500. PhiX174 DNA - (reply: 1)
501. Competent bacterial cells - (reply: 2)
502. Replacing puramycin in a pLKO1.puro - (reply: 1)
503. colony cracking for screening of positive clones - (reply: 2)
504. deletion of large fregments - (reply: 3)
505. Linearising plasmid before transfection? - (reply: 2)
506. Smear in plasmid sample - (reply: 9)
507. about a book - (reply: 1)
508. Cloning Problem! I need help - (reply: 3)
509. Help designing primers for use in In-fushion cloning - (reply: 4)
510. Repeat of desired mutation in cloning product - (reply: 1)