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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Restriction problems - (reply: 4)
482. cloning a gene in to a plasmid - (reply: 5)
483. no product on gel after restriction digestion - (reply: 3)
484. Gateway technology. - (reply: 4)
485. Primer concentration for cDNA synthesis using GSP - (reply: 1)
486. cloning from cDNA but got a much shorter PCR product - (reply: 4)
487. GeneArt vs Quikchange - (reply: 1)
488. why do some colonies keep growing in the chiller? - (reply: 7)
489. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
490. Difference between types of bacterial culture broths? - (reply: 4)
491. Co-transfection with 2 plasmids - (reply: 10)
492. Low passage HEK293?? - (reply: 3)
493. Vector Digestion Problem - (reply: 4)
494. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
495. Transformation with a big plasmid - (reply: 4)
496. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
497. Three way ligation problem - (reply: 6)
498. problems with recovery of dna from gel and ligation - (reply: 5)
499. Colony PCR and digestion with KpnI - (reply: 10)
500. Greenish tinge to bacterial culture - (reply: 3)
501. SURE E. coli competent cells - (reply: 1)
502. Ligation - (reply: 20)
503. E. coli growing on plates and bot not in LB medium - (reply: 10)
504. *** Human Insulin promoter sequence*** - (reply: 2)
505. digested plasmid - (reply: 2)
506. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
507. Subcloning - RE digest and ligation and transformation - (reply: 5)
508. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
509. Making DNA Linkers for cloning purposes - (reply: 1)
510. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)