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Top : New Forum Archives (2009-): : Molecular-Cloning
481. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
482. Large quantity heat shock trouble? - (reply: 3)
483. molecular cloning - (reply: 7)
484. Cannot see insert with colony pcr - (reply: 6)
485. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
486. help with a vector - (reply: 3)
487. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
488. Another cloning hell....no transformants - (reply: 1)
489. Agrobacterium LBA4404 transformation - (reply: 1)
490. TOPO TA Cloning Troubleshooting - (reply: 1)
491. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
492. Tricistronic vector - (reply: 1)
493. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
494. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
495. Directions of genes expression illustrations - (reply: 5)
496. Plasmid DNA at RT for a few days - (reply: 4)
497. TOPO TA Cloning - (reply: 1)
498. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
499. No insert in subcloning - (reply: 3)
500. Digest does not confirm presence of fragment - (reply: 5)
501. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
502. pGL3 Promoter replacing TSS bases - (reply: 1)
503. lipofectamine 2000:DNA complex stability - (reply: 2)
504. Problems with Ecoli glycerol stock - (reply: 6)
505. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
506. CIAP dilution - (reply: 5)
507. Problems in RNAi construction by using pUC19 - (reply: 3)
508. Unable to clone in E. coli cells - (reply: 3)
509. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
510. plasmid rearrangement or sabotage - (reply: 2)