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Top : New Forum Archives (2009-): : Molecular-Cloning
481. TOPO TA Cloning Troubleshooting - (reply: 1)
482. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
483. Tricistronic vector - (reply: 1)
484. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
485. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
486. Directions of genes expression illustrations - (reply: 5)
487. Plasmid DNA at RT for a few days - (reply: 4)
488. TOPO TA Cloning - (reply: 1)
489. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
490. No insert in subcloning - (reply: 3)
491. Digest does not confirm presence of fragment - (reply: 5)
492. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
493. pGL3 Promoter replacing TSS bases - (reply: 1)
494. lipofectamine 2000:DNA complex stability - (reply: 2)
495. Problems with Ecoli glycerol stock - (reply: 6)
496. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
497. CIAP dilution - (reply: 5)
498. Problems in RNAi construction by using pUC19 - (reply: 3)
499. Unable to clone in E. coli cells - (reply: 3)
500. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
501. plasmid rearrangement or sabotage - (reply: 2)
502. pLDR20 Vector - (reply: 1)
503. surprising behavior of transformants needs immediate advice! - (reply: 8)
504. cloning antisense promoter in pGL3Basic - (reply: 2)
505. PhiX174 DNA - (reply: 1)
506. Competent bacterial cells - (reply: 2)
507. Replacing puramycin in a pLKO1.puro - (reply: 1)
508. colony cracking for screening of positive clones - (reply: 2)
509. deletion of large fregments - (reply: 3)
510. Linearising plasmid before transfection? - (reply: 2)