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Top : New Forum Archives (2009-): : Molecular-Cloning
481. posting sequences of plasmid? - (reply: 3)
482. Restriction Enzyme Digest not working - (reply: 2)
483. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
484. Incomplete digestion of plasmid with single enzyme - (reply: 1)
485. problem with plasmid digestion - (reply: 5)
486. Difficult Ligation - Details Inside - (reply: 9)
487. pET 32 (+) - (reply: 1)
488. Alternative to pET Vectorsystem - (reply: 2)
489. pEGFP C-1/N-1 Cloning - (reply: 11)
490. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
491. Problems with growing out transformants in liquid LB - (reply: 3)
492. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
493. Ethidiumbromide - (reply: 3)
494. TRYPSIN - (reply: 4)
495. Problem with cloning - what is wrong? - (reply: 6)
496. cloning: band at different site than desired after RE digestion - (reply: 11)
497. GST TAG - (reply: 3)
498. Residues on the N terminal GST Tag - (reply: 1)
499. Where to place the polyA signal? - (reply: 1)
500. identification of target sequence - (reply: 3)
501. cloning pcr insert into plasmid - (reply: 2)
502. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
503. Large quantity heat shock trouble? - (reply: 3)
504. molecular cloning - (reply: 7)
505. Cannot see insert with colony pcr - (reply: 6)
506. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
507. help with a vector - (reply: 3)
508. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
509. Another cloning hell....no transformants - (reply: 1)
510. Agrobacterium LBA4404 transformation - (reply: 1)