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Top : New Forum Archives (2009-): : Molecular-Cloning
481. cloning pcr insert into plasmid - (reply: 2)
482. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
483. Large quantity heat shock trouble? - (reply: 3)
484. molecular cloning - (reply: 7)
485. Cannot see insert with colony pcr - (reply: 6)
486. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
487. help with a vector - (reply: 3)
488. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
489. Another cloning hell....no transformants - (reply: 1)
490. Agrobacterium LBA4404 transformation - (reply: 1)
491. TOPO TA Cloning Troubleshooting - (reply: 1)
492. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
493. Tricistronic vector - (reply: 1)
494. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
495. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
496. Directions of genes expression illustrations - (reply: 5)
497. Plasmid DNA at RT for a few days - (reply: 4)
498. TOPO TA Cloning - (reply: 1)
499. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
500. No insert in subcloning - (reply: 3)
501. Digest does not confirm presence of fragment - (reply: 5)
502. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
503. pGL3 Promoter replacing TSS bases - (reply: 1)
504. lipofectamine 2000:DNA complex stability - (reply: 2)
505. Problems with Ecoli glycerol stock - (reply: 6)
506. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
507. CIAP dilution - (reply: 5)
508. Problems in RNAi construction by using pUC19 - (reply: 3)
509. Unable to clone in E. coli cells - (reply: 3)
510. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)