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Top : New Forum Archives (2009-): : Molecular-Cloning
481. TOPO TA CLONING - LIGATION STEP - (reply: 9)
482. posting sequences of plasmid? - (reply: 3)
483. Restriction Enzyme Digest not working - (reply: 2)
484. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
485. Incomplete digestion of plasmid with single enzyme - (reply: 1)
486. problem with plasmid digestion - (reply: 5)
487. Difficult Ligation - Details Inside - (reply: 9)
488. pET 32 (+) - (reply: 1)
489. Alternative to pET Vectorsystem - (reply: 2)
490. pEGFP C-1/N-1 Cloning - (reply: 11)
491. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
492. Problems with growing out transformants in liquid LB - (reply: 3)
493. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
494. Ethidiumbromide - (reply: 3)
495. TRYPSIN - (reply: 4)
496. Problem with cloning - what is wrong? - (reply: 6)
497. cloning: band at different site than desired after RE digestion - (reply: 11)
498. GST TAG - (reply: 3)
499. Residues on the N terminal GST Tag - (reply: 1)
500. Where to place the polyA signal? - (reply: 1)
501. identification of target sequence - (reply: 3)
502. cloning pcr insert into plasmid - (reply: 2)
503. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
504. Large quantity heat shock trouble? - (reply: 3)
505. molecular cloning - (reply: 7)
506. Cannot see insert with colony pcr - (reply: 6)
507. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
508. help with a vector - (reply: 3)
509. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
510. Another cloning hell....no transformants - (reply: 1)