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Top : New Forum Archives (2009-): : Molecular-Cloning
481. restriction enzyme - (reply: 2)
482. Recreate original plasmid by cutting out insert - (reply: 2)
483. Colony-PCR workaround - (reply: 3)
484. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
485. Can I pause a bacterial culture at 4C - (reply: 2)
486. TOPO TA CLONING - LIGATION STEP - (reply: 9)
487. posting sequences of plasmid? - (reply: 3)
488. Restriction Enzyme Digest not working - (reply: 2)
489. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
490. Incomplete digestion of plasmid with single enzyme - (reply: 1)
491. problem with plasmid digestion - (reply: 5)
492. Difficult Ligation - Details Inside - (reply: 9)
493. pET 32 (+) - (reply: 1)
494. Alternative to pET Vectorsystem - (reply: 2)
495. pEGFP C-1/N-1 Cloning - (reply: 11)
496. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
497. Problems with growing out transformants in liquid LB - (reply: 3)
498. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
499. Ethidiumbromide - (reply: 3)
500. TRYPSIN - (reply: 4)
501. Problem with cloning - what is wrong? - (reply: 6)
502. cloning: band at different site than desired after RE digestion - (reply: 11)
503. GST TAG - (reply: 3)
504. Residues on the N terminal GST Tag - (reply: 1)
505. Where to place the polyA signal? - (reply: 1)
506. identification of target sequence - (reply: 3)
507. cloning pcr insert into plasmid - (reply: 2)
508. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
509. Large quantity heat shock trouble? - (reply: 3)
510. molecular cloning - (reply: 7)