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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Large quantity heat shock trouble? - (reply: 3)
482. molecular cloning - (reply: 7)
483. Cannot see insert with colony pcr - (reply: 6)
484. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
485. help with a vector - (reply: 3)
486. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
487. Another cloning hell....no transformants - (reply: 1)
488. Agrobacterium LBA4404 transformation - (reply: 1)
489. TOPO TA Cloning Troubleshooting - (reply: 1)
490. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
491. Tricistronic vector - (reply: 1)
492. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
493. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
494. Directions of genes expression illustrations - (reply: 5)
495. Plasmid DNA at RT for a few days - (reply: 4)
496. TOPO TA Cloning - (reply: 1)
497. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
498. No insert in subcloning - (reply: 3)
499. Digest does not confirm presence of fragment - (reply: 5)
500. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
501. pGL3 Promoter replacing TSS bases - (reply: 1)
502. lipofectamine 2000:DNA complex stability - (reply: 2)
503. Problems with Ecoli glycerol stock - (reply: 6)
504. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
505. CIAP dilution - (reply: 5)
506. Problems in RNAi construction by using pUC19 - (reply: 3)
507. Unable to clone in E. coli cells - (reply: 3)
508. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
509. plasmid rearrangement or sabotage - (reply: 2)
510. pLDR20 Vector - (reply: 1)