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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Problem with cloning - what is wrong? - (reply: 6)
482. cloning: band at different site than desired after RE digestion - (reply: 11)
483. GST TAG - (reply: 3)
484. Residues on the N terminal GST Tag - (reply: 1)
485. Where to place the polyA signal? - (reply: 1)
486. identification of target sequence - (reply: 3)
487. cloning pcr insert into plasmid - (reply: 2)
488. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
489. Large quantity heat shock trouble? - (reply: 3)
490. molecular cloning - (reply: 7)
491. Cannot see insert with colony pcr - (reply: 6)
492. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
493. help with a vector - (reply: 3)
494. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
495. Another cloning hell....no transformants - (reply: 1)
496. Agrobacterium LBA4404 transformation - (reply: 1)
497. TOPO TA Cloning Troubleshooting - (reply: 1)
498. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
499. Tricistronic vector - (reply: 1)
500. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
501. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
502. Directions of genes expression illustrations - (reply: 5)
503. Plasmid DNA at RT for a few days - (reply: 4)
504. TOPO TA Cloning - (reply: 1)
505. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
506. No insert in subcloning - (reply: 3)
507. Digest does not confirm presence of fragment - (reply: 5)
508. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
509. pGL3 Promoter replacing TSS bases - (reply: 1)
510. lipofectamine 2000:DNA complex stability - (reply: 2)