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Top : New Forum Archives (2009-): : Molecular-Cloning
481. GST TAG - (reply: 3)
482. Residues on the N terminal GST Tag - (reply: 1)
483. Where to place the polyA signal? - (reply: 1)
484. identification of target sequence - (reply: 3)
485. cloning pcr insert into plasmid - (reply: 2)
486. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
487. Large quantity heat shock trouble? - (reply: 3)
488. molecular cloning - (reply: 7)
489. Cannot see insert with colony pcr - (reply: 6)
490. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
491. help with a vector - (reply: 3)
492. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
493. Another cloning hell....no transformants - (reply: 1)
494. Agrobacterium LBA4404 transformation - (reply: 1)
495. TOPO TA Cloning Troubleshooting - (reply: 1)
496. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
497. Tricistronic vector - (reply: 1)
498. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
499. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
500. Directions of genes expression illustrations - (reply: 5)
501. Plasmid DNA at RT for a few days - (reply: 4)
502. TOPO TA Cloning - (reply: 1)
503. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
504. No insert in subcloning - (reply: 3)
505. Digest does not confirm presence of fragment - (reply: 5)
506. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
507. pGL3 Promoter replacing TSS bases - (reply: 1)
508. lipofectamine 2000:DNA complex stability - (reply: 2)
509. Problems with Ecoli glycerol stock - (reply: 6)
510. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)