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Top : New Forum Archives (2009-): : Molecular-Cloning
481. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
482. Incomplete digestion of plasmid with single enzyme - (reply: 1)
483. problem with plasmid digestion - (reply: 5)
484. Difficult Ligation - Details Inside - (reply: 9)
485. pET 32 (+) - (reply: 1)
486. Alternative to pET Vectorsystem - (reply: 2)
487. pEGFP C-1/N-1 Cloning - (reply: 11)
488. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
489. Problems with growing out transformants in liquid LB - (reply: 3)
490. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
491. Ethidiumbromide - (reply: 3)
492. TRYPSIN - (reply: 4)
493. Problem with cloning - what is wrong? - (reply: 6)
494. cloning: band at different site than desired after RE digestion - (reply: 11)
495. GST TAG - (reply: 3)
496. Residues on the N terminal GST Tag - (reply: 1)
497. Where to place the polyA signal? - (reply: 1)
498. identification of target sequence - (reply: 3)
499. cloning pcr insert into plasmid - (reply: 2)
500. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
501. Large quantity heat shock trouble? - (reply: 3)
502. molecular cloning - (reply: 7)
503. Cannot see insert with colony pcr - (reply: 6)
504. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
505. help with a vector - (reply: 3)
506. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
507. Another cloning hell....no transformants - (reply: 1)
508. Agrobacterium LBA4404 transformation - (reply: 1)
509. TOPO TA Cloning Troubleshooting - (reply: 1)
510. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)