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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Where to place the polyA signal? - (reply: 1)
482. identification of target sequence - (reply: 3)
483. cloning pcr insert into plasmid - (reply: 2)
484. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
485. Large quantity heat shock trouble? - (reply: 3)
486. molecular cloning - (reply: 7)
487. Cannot see insert with colony pcr - (reply: 6)
488. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
489. help with a vector - (reply: 3)
490. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
491. Another cloning hell....no transformants - (reply: 1)
492. Agrobacterium LBA4404 transformation - (reply: 1)
493. TOPO TA Cloning Troubleshooting - (reply: 1)
494. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
495. Tricistronic vector - (reply: 1)
496. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
497. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
498. Directions of genes expression illustrations - (reply: 5)
499. Plasmid DNA at RT for a few days - (reply: 4)
500. TOPO TA Cloning - (reply: 1)
501. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
502. No insert in subcloning - (reply: 3)
503. Digest does not confirm presence of fragment - (reply: 5)
504. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
505. pGL3 Promoter replacing TSS bases - (reply: 1)
506. lipofectamine 2000:DNA complex stability - (reply: 2)
507. Problems with Ecoli glycerol stock - (reply: 6)
508. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
509. CIAP dilution - (reply: 5)
510. Problems in RNAi construction by using pUC19 - (reply: 3)