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Top : New Forum Archives (2009-): : Molecular-Cloning
481. Ethidiumbromide - (reply: 3)
482. TRYPSIN - (reply: 4)
483. Problem with cloning - what is wrong? - (reply: 6)
484. cloning: band at different site than desired after RE digestion - (reply: 11)
485. GST TAG - (reply: 3)
486. Residues on the N terminal GST Tag - (reply: 1)
487. Where to place the polyA signal? - (reply: 1)
488. identification of target sequence - (reply: 3)
489. cloning pcr insert into plasmid - (reply: 2)
490. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
491. Large quantity heat shock trouble? - (reply: 3)
492. molecular cloning - (reply: 7)
493. Cannot see insert with colony pcr - (reply: 6)
494. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
495. help with a vector - (reply: 3)
496. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
497. Another cloning hell....no transformants - (reply: 1)
498. Agrobacterium LBA4404 transformation - (reply: 1)
499. TOPO TA Cloning Troubleshooting - (reply: 1)
500. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
501. Tricistronic vector - (reply: 1)
502. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
503. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
504. Directions of genes expression illustrations - (reply: 5)
505. Plasmid DNA at RT for a few days - (reply: 4)
506. TOPO TA Cloning - (reply: 1)
507. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
508. No insert in subcloning - (reply: 3)
509. Digest does not confirm presence of fragment - (reply: 5)
510. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)