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Top : New Forum Archives (2009-): : Molecular-Cloning
1411. Help with transformation - (reply: 3)
1412. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1413. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1414. Help cloning!!! - As usual cloning is not working! (reply: 11)
1415. pSuper cloning problems - (reply: 1)
1416. problem with plasmid extraction - (reply: 3)
1417. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1418. Smear in my restriction digest - (reply: 12)
1419. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1420. How to transfect two plasmids in a cell - (reply: 10)
1421. Double Digest using enzymes from diff companies - (reply: 4)
1422. Ligation problem (no colonies, but on negative control) - (reply: 3)
1423. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1424. SDM - (reply: 3)
1425. inframe problem - (reply: 1)
1426. Touchdown PCR - (reply: 1)
1427. Adding A tails? - (reply: 3)
1428. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1429. Determine annealing temperature of primers - (reply: 14)
1430. Bacteria pellet has almost-black region - (reply: 10)
1431. no ligation !!!!! - (reply: 6)
1432. How to clean up restriction enzyme digestion - (reply: 8)
1433. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1434. GPCR - (reply: 1)
1435. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1436. finding the suitable RE - first timer (reply: 6)
1437. TOPO TA Problems - TOPO TA Problems (reply: 8)
1438. about antibiotic addition - (reply: 4)
1439. about chloramphenicol and L.lactis - (reply: 1)
1440. ligation - (reply: 5)