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Top : New Forum Archives (2009-): : Molecular-Cloning
1411. optimum temperature for ligation - (reply: 14)
1412. Please Help with Lentiviral Cloning - (reply: 1)
1413. plasmid-rest. dign. - (reply: 2)
1414. Help with cloning!. - Cloning problems (reply: 20)
1415. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
1416. Cloning PCR fragment - (reply: 1)
1417. molecular cloning - (reply: 2)
1418. DNA cloning - (reply: 4)
1419. Help with transformation - (reply: 3)
1420. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1421. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1422. Help cloning!!! - As usual cloning is not working! (reply: 11)
1423. pSuper cloning problems - (reply: 1)
1424. problem with plasmid extraction - (reply: 3)
1425. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1426. Smear in my restriction digest - (reply: 12)
1427. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1428. How to transfect two plasmids in a cell - (reply: 10)
1429. Double Digest using enzymes from diff companies - (reply: 4)
1430. Ligation problem (no colonies, but on negative control) - (reply: 3)
1431. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1432. SDM - (reply: 3)
1433. inframe problem - (reply: 1)
1434. Touchdown PCR - (reply: 1)
1435. Adding A tails? - (reply: 3)
1436. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1437. Determine annealing temperature of primers - (reply: 14)
1438. Bacteria pellet has almost-black region - (reply: 10)
1439. no ligation !!!!! - (reply: 6)
1440. How to clean up restriction enzyme digestion - (reply: 8)