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Top : New Forum Archives (2009-): : Molecular-Cloning
1411. Smear in my restriction digest - (reply: 12)
1412. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1413. How to transfect two plasmids in a cell - (reply: 10)
1414. Double Digest using enzymes from diff companies - (reply: 4)
1415. Ligation problem (no colonies, but on negative control) - (reply: 3)
1416. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1417. SDM - (reply: 3)
1418. inframe problem - (reply: 1)
1419. Touchdown PCR - (reply: 1)
1420. Adding A tails? - (reply: 3)
1421. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1422. Determine annealing temperature of primers - (reply: 14)
1423. Bacteria pellet has almost-black region - (reply: 10)
1424. no ligation !!!!! - (reply: 6)
1425. How to clean up restriction enzyme digestion - (reply: 8)
1426. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1427. GPCR - (reply: 1)
1428. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1429. finding the suitable RE - first timer (reply: 6)
1430. TOPO TA Problems - TOPO TA Problems (reply: 8)
1431. about antibiotic addition - (reply: 4)
1432. about chloramphenicol and L.lactis - (reply: 1)
1433. ligation - (reply: 5)
1434. Losing insert from pGEM? - (reply: 2)
1435. How to concentrate lenti or retroviral particules - (reply: 3)
1436. wierd clot in LB culture - (reply: 5)
1437. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1438. Not getting enough colony - (reply: 4)
1439. Ligation problem - (reply: 9)
1440. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)