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Top : New Forum Archives (2009-): : Molecular-Cloning
1411. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1412. Blunt-end cloning problem - (reply: 6)
1413. Supercoiling blocking restriction site? - (reply: 6)
1414. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1415. problem in gene cloning - (reply: 4)
1416. Odd point mutations - (reply: 23)
1417. Plasmid linearization by PCR - (reply: 4)
1418. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1419. can't make the cloning work - (reply: 3)
1420. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1421. cloning help - (reply: 9)
1422. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1423. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1424. Ligation trouble continues - subcloning ligation failure (reply: 9)
1425. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1426. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1427. colony PCR - (reply: 8)
1428. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1429. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1430. Truncated sequence - (reply: 1)
1431. gateway cloning - (reply: 3)
1432. Purifying and cloning 69nt product - (reply: 5)
1433. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1434. ligatin issues - (reply: 1)
1435. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1436. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1437. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1438. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1439. A gateway cloning expression vector question - (reply: 1)
1440. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)