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Top : New Forum Archives (2009-): : Molecular-Cloning
1411. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1412. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1413. cloning problem - molecular cloning (reply: 1)
1414. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1415. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1416. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1417. choosing restriction sites - (reply: 5)
1418. Tag insertion - (reply: 5)
1419. Transduction - (reply: 3)
1420. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1421. my colonies don't have the insert, how is this possible? - (reply: 7)
1422. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1423. PCR problem from transformed TOPO TA vector - (reply: 3)
1424. nondenaturing PAGE to separate dna - (reply: 4)
1425. why sometimes big or small colonies? - (reply: 16)
1426. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1427. wrong bands after T-vector cloning - (reply: 3)
1428. urgent help regarding wanner method trouble shooting - (reply: 2)
1429. two step digestion - (reply: 6)
1430. Storage of invitrogen top10 cells - (reply: 11)
1431. Self-Ligation of digested vector - (reply: 2)
1432. Help with cloning - (reply: 6)
1433. Ligation issue: Does vector size matter? - (reply: 10)
1434. Help for T-A cloning - (reply: 15)
1435. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1436. Some colonies, but not enough... - (reply: 7)
1437. transformation problem - (reply: 3)
1438. Plasmid sequence - (reply: 1)
1439. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1440. help wih the ligation - (reply: 7)