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Top : New Forum Archives (2009-): : Molecular-Cloning
1411. ligation question - three-point ligation (reply: 9)
1412. Problems cloning Hairpin vector - (reply: 3)
1413. request pIRES2-EGFP - (reply: 2)
1414. different digestion pattern for same clone - (reply: 4)
1415. Problem with DNA purification from agarose gel - (reply: 3)
1416. cloning trouble - (reply: 15)
1417. No band in the gel after ligation - (reply: 6)
1418. Troubleshooting Digest, ligation, transformation - (reply: 7)
1419. Ideal incubation time - (reply: 3)
1420. Competent DH5 alpha - (reply: 4)
1421. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1422. Quick ? about Enzyme Buffers - (reply: 3)
1423. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1424. Inserts too small after transformation - transformation (reply: 3)
1425. glycerol stocks - storage (reply: 2)
1426. growth of colonies after ligation - (reply: 2)
1427. cDNA cloning problem - (reply: 1)
1428. Any good way to remove DNase? - (reply: 5)
1429. DH5a comp cells - (reply: 5)
1430. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1431. Monocot promoters - (reply: 1)
1432. ligation problem - (reply: 11)
1433. How Gateway cloning system works? - (reply: 1)
1434. Chemically competent to electrocompetent DH5alpha - (reply: 1)
1435. Tetracycline concnetration in selection - (reply: 1)
1436. pCS2 - (reply: 2)
1437. What does this look like to you? - (reply: 23)
1438. ds-oligo cloning/ligation issues - (reply: 18)
1439. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
1440. CIP required for double digestion of plasmid? - (reply: 3)