Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1411. Cloning PCR fragment - (reply: 1)
1412. molecular cloning - (reply: 2)
1413. DNA cloning - (reply: 4)
1414. Help with transformation - (reply: 3)
1415. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1416. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1417. Help cloning!!! - As usual cloning is not working! (reply: 11)
1418. pSuper cloning problems - (reply: 1)
1419. problem with plasmid extraction - (reply: 3)
1420. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1421. Smear in my restriction digest - (reply: 12)
1422. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1423. How to transfect two plasmids in a cell - (reply: 10)
1424. Double Digest using enzymes from diff companies - (reply: 4)
1425. Ligation problem (no colonies, but on negative control) - (reply: 3)
1426. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1427. SDM - (reply: 3)
1428. inframe problem - (reply: 1)
1429. Touchdown PCR - (reply: 1)
1430. Adding A tails? - (reply: 3)
1431. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1432. Determine annealing temperature of primers - (reply: 14)
1433. Bacteria pellet has almost-black region - (reply: 10)
1434. no ligation !!!!! - (reply: 6)
1435. How to clean up restriction enzyme digestion - (reply: 8)
1436. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1437. GPCR - (reply: 1)
1438. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1439. finding the suitable RE - first timer (reply: 6)
1440. TOPO TA Problems - TOPO TA Problems (reply: 8)