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Top : New Forum Archives (2009-): : Molecular-Cloning
991. Digesting large amount of plasmid - (reply: 4)
992. cleanup of digestion before ligation - (reply: 6)
993. transformation efficiency - (reply: 7)
994. transformed bacteria doesnt grow - (reply: 1)
995. TOPO Cloning - (reply: 5)
996. blunt ligation - (reply: 4)
997. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
998. About Laminin 5'UTR 's function - (reply: 1)
999. Vectors with special MCS - (reply: 5)
1000. Cloning <100 bp fragments into a vector - (reply: 7)
1001. Help! my plasmid size changed after ligation - (reply: 5)
1002. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1003. Restriction digestion of gDNA for phage library - (reply: 5)
1004. how i can get insert back? - (reply: 1)
1005. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1006. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1007. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1008. mammalian expression vector - (reply: 2)
1009. Electroporation doesn't like my plasmid??? - (reply: 4)
1010. low yeild fo RNA after invitro transcription - (reply: 4)
1011. No expresion of transgene - Retroviral vector (reply: 1)
1012. plasmids - plasmids for stable cell line construction (reply: 2)
1013. empty vectors more than the insert - (reply: 6)
1014. Restriction enzyme digestion problem - (reply: 2)
1015. low copy plasmid - how to improve the yield? (reply: 1)
1016. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1017. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1018. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1019. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1020. Cloning Help!! - It is getting very annoying... (reply: 13)