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Top : New Forum Archives (2009-): : Molecular-Cloning
991. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
992. Topo cloning or pGEM-T easy ? - (reply: 3)
993. Problems with qiaquick gel purification kit - cloning (reply: 10)
994. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
995. integration of a gene per homologous recombination - (reply: 5)
996. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
997. Antarctic Phosphatase treatment - (reply: 5)
998. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
999. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1000. Plasmid out of BL21 for mutagensis - (reply: 3)
1001. no colony growth - (reply: 4)
1002. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1003. Effect of a DNA prep on DE3 - (reply: 2)
1004. Competent cell storage for long term use - (reply: 2)
1005. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1006. New to sequencing, primer design - (reply: 2)
1007. ta cloning problem - (reply: 2)
1008. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1009. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
1010. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1011. Storage of Bacterial Culture at 4 degrees - (reply: 2)
1012. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1013. rna isolation - (reply: 1)
1014. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
1015. Molecular Cloning od Recombinant DNA - (reply: 3)
1016. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
1017. looking for binding site - software? (reply: 1)
1018. Insertion of loxp/avoiding SINE - (reply: 1)
1019. Question: Ligation Problem.... - (reply: 2)
1020. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)