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Top : New Forum Archives (2009-): : Molecular-Cloning
991. what is the difference between DH10B and Top10 - (reply: 1)
992. problems encountered when working on a large plasmid - (reply: 2)
993. make a Mega plasmid mutation - (reply: 1)
994. Cloning - (reply: 1)
995. Copy-number calculation formula? - (reply: 1)
996. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
997. low melt agarose - (reply: 1)
998. restriction digest - smear - (reply: 1)
999. designing of primers - (reply: 2)
1000. Propagating ccdB survival cells - (reply: 6)
1001. Bicistronic vector vs "normal" vector - (reply: 5)
1002. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1003. Can't seem to get insert into vector... please help - (reply: 4)
1004. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1005. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1006. Topo cloning or pGEM-T easy ? - (reply: 3)
1007. Problems with qiaquick gel purification kit - cloning (reply: 10)
1008. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1009. integration of a gene per homologous recombination - (reply: 5)
1010. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1011. Antarctic Phosphatase treatment - (reply: 5)
1012. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1013. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1014. Plasmid out of BL21 for mutagensis - (reply: 3)
1015. no colony growth - (reply: 4)
1016. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1017. Effect of a DNA prep on DE3 - (reply: 2)
1018. Competent cell storage for long term use - (reply: 2)
1019. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1020. New to sequencing, primer design - (reply: 2)