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Top : New Forum Archives (2009-): : Molecular-Cloning
991. In-fusion joining few parts together - possible? ideas? (reply: 3)
992. TOPO cloning problem - (reply: 4)
993. How to delete a domain in a gene? - cloned plasmid (reply: 12)
994. Red/ET recombination - (reply: 2)
995. multiple origins of replication - (reply: 2)
996. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
997. Single Restriction Site cloning - Cloning (reply: 6)
998. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
999. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1000. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1001. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1002. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1003. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1004. Agarose gel electrophoresis question - (reply: 1)
1005. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1006. My white colonies turned blue in frig! Why? - (reply: 6)
1007. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1008. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1009. Dephosphorylation of vector - (reply: 8)
1010. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1011. rna isolation - (reply: 1)
1012. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1013. So many colonies! - (reply: 8)
1014. Large vector cloning-recombination problem? - (reply: 1)
1015. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1016. selecting recombinants - (reply: 1)
1017. colony PCR for subcloning gene - (reply: 1)
1018. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1019. cloning of a 20 kb insert - (reply: 2)
1020. DNA quantification after gel purification - (reply: 6)