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991. mutagenesis expert come in~ - (reply: 11)
992. Subcloning - (reply: 3)
993. Sequencing different colonies - (reply: 5)
994. detection of restriction? - (reply: 1)
995. problem in pcr and purification - (reply: 3)
996. bacterial stock preparation - (reply: 3)
997. Problem with site directed mutagenesis - (reply: 7)
998. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
999. Forgot to add X-gal/IPTG - (reply: 8)
1000. after re-isolation of vector, get single band - (reply: 4)
1001. Subcloning into pMIR-REPORT-luc - (reply: 7)
1002. Restriction digestion troubleshooting - (reply: 10)
1003. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1004. Which digestion enzyme? - (reply: 1)
1005. Cloning big fragment into smaller vector - (reply: 3)
1006. from where can i get the P1 phage - (reply: 1)
1007. multi way ligation or long range pcr? - (reply: 1)
1008. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1009. list of sequence to be added before RE site - (reply: 3)
1010. plasmid, BAC, or cosmid? - (reply: 3)
1011. Wierd trouble - dunno where my insert is?? (reply: 3)
1012. Large Molecular weight DNA Extarction - (reply: 7)
1013. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1014. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1015. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1016. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1017. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1018. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1019. Vector sequence included in (PCR) amplified insert - (reply: 2)
1020. COMPETENT CELL PREPARATION - (reply: 6)
992. Subcloning - (reply: 3)
993. Sequencing different colonies - (reply: 5)
994. detection of restriction? - (reply: 1)
995. problem in pcr and purification - (reply: 3)
996. bacterial stock preparation - (reply: 3)
997. Problem with site directed mutagenesis - (reply: 7)
998. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
999. Forgot to add X-gal/IPTG - (reply: 8)
1000. after re-isolation of vector, get single band - (reply: 4)
1001. Subcloning into pMIR-REPORT-luc - (reply: 7)
1002. Restriction digestion troubleshooting - (reply: 10)
1003. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1004. Which digestion enzyme? - (reply: 1)
1005. Cloning big fragment into smaller vector - (reply: 3)
1006. from where can i get the P1 phage - (reply: 1)
1007. multi way ligation or long range pcr? - (reply: 1)
1008. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1009. list of sequence to be added before RE site - (reply: 3)
1010. plasmid, BAC, or cosmid? - (reply: 3)
1011. Wierd trouble - dunno where my insert is?? (reply: 3)
1012. Large Molecular weight DNA Extarction - (reply: 7)
1013. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1014. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1015. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1016. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1017. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1018. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1019. Vector sequence included in (PCR) amplified insert - (reply: 2)
1020. COMPETENT CELL PREPARATION - (reply: 6)