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Top : New Forum Archives (2009-): : Molecular-Cloning
991. ligation problem - (reply: 6)
992. Failed Ligation - (reply: 2)
993. cloning of GroEL - (reply: 3)
994. Ligation problem - (reply: 9)
995. Problems with High Background with Gateway Cloning - (reply: 1)
996. Mutagenesis troubles - (reply: 2)
997. ligation/transformation failed - (reply: 7)
998. Transfecting primary cells with plasmid - (reply: 1)
999. Extra bands after QIAGEN spin miniprep - (reply: 3)
1000. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1001. The use of hygromycin - (reply: 2)
1002. QUESTION about cloning - (reply: 5)
1003. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1004. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1005. Ligation screening - (reply: 4)
1006. Double digestion and ligation problem - (reply: 1)
1007. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1008. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1009. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1010. CMV promoter - (reply: 2)
1011. insert in the reverse orientation after transformation - (reply: 5)
1012. cDNA as PCR template - (reply: 1)
1013. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1014. T4 ligase buffer - (reply: 4)
1015. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1016. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1017. ligation problems - no colonies at the end!!!!! (reply: 4)
1018. Good stable mammalian promoter - (reply: 1)
1019. Self-ligation from a Double Digest - (reply: 4)
1020. Strange frameshift question - (reply: 2)