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Top : New Forum Archives (2009-): : Molecular-Cloning
991. make a Mega plasmid mutation - (reply: 1)
992. Cloning - (reply: 1)
993. Copy-number calculation formula? - (reply: 1)
994. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
995. low melt agarose - (reply: 1)
996. restriction digest - smear - (reply: 1)
997. designing of primers - (reply: 2)
998. Propagating ccdB survival cells - (reply: 6)
999. Bicistronic vector vs "normal" vector - (reply: 5)
1000. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1001. Can't seem to get insert into vector... please help - (reply: 4)
1002. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1003. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1004. Topo cloning or pGEM-T easy ? - (reply: 3)
1005. Problems with qiaquick gel purification kit - cloning (reply: 10)
1006. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1007. integration of a gene per homologous recombination - (reply: 5)
1008. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1009. Antarctic Phosphatase treatment - (reply: 5)
1010. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1011. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1012. Plasmid out of BL21 for mutagensis - (reply: 3)
1013. no colony growth - (reply: 4)
1014. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1015. Effect of a DNA prep on DE3 - (reply: 2)
1016. Competent cell storage for long term use - (reply: 2)
1017. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1018. New to sequencing, primer design - (reply: 2)
1019. ta cloning problem - (reply: 2)
1020. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)