Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
991. restriction digest - smear - (reply: 1)
992. designing of primers - (reply: 2)
993. Propagating ccdB survival cells - (reply: 6)
994. Bicistronic vector vs "normal" vector - (reply: 5)
995. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
996. Can't seem to get insert into vector... please help - (reply: 4)
997. plasmid cloning - i need help.. can anyone answer me (reply: 5)
998. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
999. Topo cloning or pGEM-T easy ? - (reply: 3)
1000. Problems with qiaquick gel purification kit - cloning (reply: 10)
1001. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1002. integration of a gene per homologous recombination - (reply: 5)
1003. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1004. Antarctic Phosphatase treatment - (reply: 5)
1005. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1006. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1007. Plasmid out of BL21 for mutagensis - (reply: 3)
1008. no colony growth - (reply: 4)
1009. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1010. Effect of a DNA prep on DE3 - (reply: 2)
1011. Competent cell storage for long term use - (reply: 2)
1012. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1013. New to sequencing, primer design - (reply: 2)
1014. ta cloning problem - (reply: 2)
1015. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1016. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
1017. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1018. Storage of Bacterial Culture at 4 degrees - (reply: 2)
1019. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1020. rna isolation - (reply: 1)