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Top : New Forum Archives (2009-): : Molecular-Cloning
991. Unusual band in transformants - (reply: 2)
992. ligation--pls help! - (reply: 10)
993. bizarre digestion:) - (reply: 2)
994. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
995. metagenomic library consturction - BAC vectors (reply: 1)
996. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
997. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
998. Chromosomal recombination with BL21? - (reply: 2)
999. Restriction Digestion!! - (reply: 5)
1000. digestion - restriction enzymes (reply: 2)
1001. How to prevent self ligation - selfligation (reply: 11)
1002. what is the difference between DH10B and Top10 - (reply: 1)
1003. problems encountered when working on a large plasmid - (reply: 2)
1004. make a Mega plasmid mutation - (reply: 1)
1005. Cloning - (reply: 1)
1006. Copy-number calculation formula? - (reply: 1)
1007. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
1008. low melt agarose - (reply: 1)
1009. restriction digest - smear - (reply: 1)
1010. designing of primers - (reply: 2)
1011. Propagating ccdB survival cells - (reply: 6)
1012. Bicistronic vector vs "normal" vector - (reply: 5)
1013. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1014. Can't seem to get insert into vector... please help - (reply: 4)
1015. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1016. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1017. Topo cloning or pGEM-T easy ? - (reply: 3)
1018. Problems with qiaquick gel purification kit - cloning (reply: 10)
1019. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1020. integration of a gene per homologous recombination - (reply: 5)