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Top : New Forum Archives (2009-): : Molecular-Cloning
991. Cloning - (reply: 1)
992. Copy-number calculation formula? - (reply: 1)
993. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
994. low melt agarose - (reply: 1)
995. restriction digest - smear - (reply: 1)
996. designing of primers - (reply: 2)
997. Propagating ccdB survival cells - (reply: 6)
998. Bicistronic vector vs "normal" vector - (reply: 5)
999. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1000. Can't seem to get insert into vector... please help - (reply: 4)
1001. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1002. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1003. Topo cloning or pGEM-T easy ? - (reply: 3)
1004. Problems with qiaquick gel purification kit - cloning (reply: 10)
1005. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1006. integration of a gene per homologous recombination - (reply: 5)
1007. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1008. Antarctic Phosphatase treatment - (reply: 5)
1009. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1010. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1011. Plasmid out of BL21 for mutagensis - (reply: 3)
1012. no colony growth - (reply: 4)
1013. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1014. Effect of a DNA prep on DE3 - (reply: 2)
1015. Competent cell storage for long term use - (reply: 2)
1016. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1017. New to sequencing, primer design - (reply: 2)
1018. ta cloning problem - (reply: 2)
1019. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1020. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)