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Top : New Forum Archives (2009-): : Molecular-Cloning
991. metagenomic library consturction - BAC vectors (reply: 1)
992. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
993. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
994. Chromosomal recombination with BL21? - (reply: 2)
995. Restriction Digestion!! - (reply: 5)
996. digestion - restriction enzymes (reply: 2)
997. How to prevent self ligation - selfligation (reply: 11)
998. what is the difference between DH10B and Top10 - (reply: 1)
999. problems encountered when working on a large plasmid - (reply: 2)
1000. make a Mega plasmid mutation - (reply: 1)
1001. Cloning - (reply: 1)
1002. Copy-number calculation formula? - (reply: 1)
1003. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
1004. low melt agarose - (reply: 1)
1005. restriction digest - smear - (reply: 1)
1006. designing of primers - (reply: 2)
1007. Propagating ccdB survival cells - (reply: 6)
1008. Bicistronic vector vs "normal" vector - (reply: 5)
1009. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1010. Can't seem to get insert into vector... please help - (reply: 4)
1011. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1012. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1013. Topo cloning or pGEM-T easy ? - (reply: 3)
1014. Problems with qiaquick gel purification kit - cloning (reply: 10)
1015. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1016. integration of a gene per homologous recombination - (reply: 5)
1017. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1018. Antarctic Phosphatase treatment - (reply: 5)
1019. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1020. phage DNA - DNA wont migrate on agarose gel (reply: 3)