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Top : New Forum Archives (2009-): : Molecular-Cloning
991. Plasmid out of BL21 for mutagensis - (reply: 3)
992. no colony growth - (reply: 4)
993. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
994. Effect of a DNA prep on DE3 - (reply: 2)
995. Competent cell storage for long term use - (reply: 2)
996. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
997. New to sequencing, primer design - (reply: 2)
998. ta cloning problem - (reply: 2)
999. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1000. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
1001. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1002. Storage of Bacterial Culture at 4 degrees - (reply: 2)
1003. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1004. rna isolation - (reply: 1)
1005. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
1006. Molecular Cloning od Recombinant DNA - (reply: 3)
1007. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
1008. looking for binding site - software? (reply: 1)
1009. Insertion of loxp/avoiding SINE - (reply: 1)
1010. Question: Ligation Problem.... - (reply: 2)
1011. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
1012. Topo directional cloning - pENTR/D-TOPO (reply: 1)
1013. trouble in TOPO TA sequencing - (reply: 1)
1014. Large DNA extraction from an agarose gel - (reply: 8)
1015. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1016. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1017. Ligation problem, religation of backbone without any insert? - (reply: 3)
1018. problem with pLK01 plasmid ligation - (reply: 2)
1019. Mini prep streaking - basic question (reply: 3)
1020. In-fusion joining few parts together - possible? ideas? (reply: 3)