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Top : New Forum Archives (2009-): : Molecular-Cloning
991. what is site-specific integration - (reply: 2)
992. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
993. double transformations - (reply: 1)
994. Unusual band in transformants - (reply: 2)
995. ligation--pls help! - (reply: 10)
996. bizarre digestion:) - (reply: 2)
997. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
998. metagenomic library consturction - BAC vectors (reply: 1)
999. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
1000. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
1001. Chromosomal recombination with BL21? - (reply: 2)
1002. Restriction Digestion!! - (reply: 5)
1003. digestion - restriction enzymes (reply: 2)
1004. How to prevent self ligation - selfligation (reply: 11)
1005. what is the difference between DH10B and Top10 - (reply: 1)
1006. problems encountered when working on a large plasmid - (reply: 2)
1007. make a Mega plasmid mutation - (reply: 1)
1008. Cloning - (reply: 1)
1009. Copy-number calculation formula? - (reply: 1)
1010. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
1011. low melt agarose - (reply: 1)
1012. restriction digest - smear - (reply: 1)
1013. designing of primers - (reply: 2)
1014. Propagating ccdB survival cells - (reply: 6)
1015. Bicistronic vector vs "normal" vector - (reply: 5)
1016. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1017. Can't seem to get insert into vector... please help - (reply: 4)
1018. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1019. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1020. Topo cloning or pGEM-T easy ? - (reply: 3)