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Top : New Forum Archives (2009-): : Molecular-Cloning
991. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
992. low melt agarose - (reply: 1)
993. restriction digest - smear - (reply: 1)
994. designing of primers - (reply: 2)
995. Propagating ccdB survival cells - (reply: 6)
996. Bicistronic vector vs "normal" vector - (reply: 5)
997. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
998. Can't seem to get insert into vector... please help - (reply: 4)
999. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1000. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1001. Topo cloning or pGEM-T easy ? - (reply: 3)
1002. Problems with qiaquick gel purification kit - cloning (reply: 10)
1003. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1004. integration of a gene per homologous recombination - (reply: 5)
1005. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1006. Antarctic Phosphatase treatment - (reply: 5)
1007. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1008. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1009. Plasmid out of BL21 for mutagensis - (reply: 3)
1010. no colony growth - (reply: 4)
1011. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1012. Effect of a DNA prep on DE3 - (reply: 2)
1013. Competent cell storage for long term use - (reply: 2)
1014. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1015. New to sequencing, primer design - (reply: 2)
1016. ta cloning problem - (reply: 2)
1017. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1018. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
1019. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1020. Storage of Bacterial Culture at 4 degrees - (reply: 2)