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Top : New Forum Archives (2009-): : Molecular-Cloning
331. T7 and M13 primers two band amplification - (reply: 3)
332. Type of polymerases compatible for TA cloning - (reply: 7)
333. Running undigested plasmids on gel - (reply: 2)
334. Molecular Cloning Sambrook - (reply: 1)
335. What is the membrane made of in DNA purification kits - (reply: 3)
336. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
337. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
338. Removing Introns from a DNA sequence - (reply: 1)
339. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
340. without RE site in PCR product - (reply: 5)
341. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
342. excision of fragment during cloning - (reply: 4)
343. Unusual cloning issue... anyone else?? - (reply: 4)
344. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
345. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
346. How to avoid RNA contamination in DNA samples:? - (reply: 1)
347. Wavy Lines in Gel - (reply: 2)
348. Question Relating to LacZ Disruption - (reply: 4)
349. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
350. Site directed mutagenesis - (reply: 5)
351. PCR insert - in frame? - (reply: 2)
352. Whole plasmid amplification by PCR - (reply: 2)
353. colony PCR after transformation - (reply: 1)
354. For promoter assay, which vector I should use? - (reply: 5)
355. Sequencing of the cloned plasmid - (reply: 5)
356. 4 way cloning into TA Vector - (reply: 10)
357. I get band double the size what does it mean - (reply: 3)
358. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
359. Neeed Help , cloning Confirmation ?! - (reply: 5)
360. Puzzling Cloning Problem With a Specific Vector - (reply: 1)