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Top : New Forum Archives (2009-): : Molecular-Cloning
331. 4 way cloning into TA Vector - (reply: 10)
332. I get band double the size what does it mean - (reply: 3)
333. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
334. Neeed Help , cloning Confirmation ?! - (reply: 5)
335. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
336. Can I quantify restricted plasmid and oligos? - (reply: 3)
337. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
338. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
339. TOPO TA ligation problems - (reply: 6)
340. Luciferase assay problem - (reply: 2)
341. Double digestion with BamHI and BglII - (reply: 4)
342. nicked linear DNA - (reply: 5)
343. Selection of E.coli competent cells - (reply: 2)
344. Double strand cdna synthysis - (reply: 4)
345. mutagenic primers with very high GC content. - (reply: 3)
346. TA Cloning of ds cDNA - (reply: 6)
347. Promoter distance from ATG - (reply: 3)
348. Measuring Fluorescene of RFP - (reply: 7)
349. Smallest possible insert size for Ligation reaction - (reply: 2)
350. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
351. Extraction of DNA from termite gut flagellates - (reply: 7)
352. Enzymes and buffers from different companies - (reply: 6)
353. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
354. faint band after plasmid extraction - (reply: 7)
355. Adaptor ligation - (reply: 9)
356. self ligation - (reply: 1)
357. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
358. Cloning large transgenes - (reply: 2)
359. In-fusion HD cloning - (reply: 3)
360. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)