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Top : New Forum Archives (2009-): : Molecular-Cloning
331. recombinant protein form - (reply: 2)
332. restricted PCR plasmid runs slower - (reply: 2)
333. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
334. High frequency of vector religation - (reply: 10)
335. Cloned but not expressing! - (reply: 8)
336. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
337. DNA ligation and trasformation - (reply: 6)
338. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
339. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
340. How to set up simultaneous digestion? - (reply: 3)
341. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
342. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
343. Freeze transformation mixture? - (reply: 2)
344. Cutting my plasmid made it larger - (reply: 7)
345. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
346. Amplification of CAG promoter problems - (reply: 4)
347. restriction enzyme - (reply: 2)
348. Recreate original plasmid by cutting out insert - (reply: 2)
349. Colony-PCR workaround - (reply: 3)
350. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
351. Can I pause a bacterial culture at 4C - (reply: 2)
352. TOPO TA CLONING - LIGATION STEP - (reply: 9)
353. posting sequences of plasmid? - (reply: 3)
354. Restriction Enzyme Digest not working - (reply: 2)
355. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
356. Incomplete digestion of plasmid with single enzyme - (reply: 1)
357. problem with plasmid digestion - (reply: 5)
358. Difficult Ligation - Details Inside - (reply: 9)
359. pET 32 (+) - (reply: 1)
360. Alternative to pET Vectorsystem - (reply: 2)