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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
332. Do I need to sequence after digestion? - (reply: 9)
333. quick change mutagenesis...... - (reply: 15)
334. Problem with double digestion: one enzyme is not working - (reply: 8)
335. Transient Transfection Controls (Gateway Technology) - (reply: 4)
336. Problems with crossover PCR - (reply: 2)
337. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
338. Two genes, same MCS - (reply: 1)
339. Including ATG in cloning? - (reply: 1)
340. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
341. Low copy vector for library generation - (reply: 2)
342. Can't find a recombinant after ligating - (reply: 4)
343. problems with plasmid miniprep - (reply: 8)
344. gene cloning - (reply: 1)
345. Is ligation really necessary? - (reply: 6)
346. problem with ligation of linker/adaptor - (reply: 2)
347. GFP -weak signal -why? - (reply: 1)
348. Ligation troubleshooting! Please help! - (reply: 5)
349. Truncated gene Cloning - (reply: 1)
350. Screening for ligation and transformation result - (reply: 2)
351. Problem with 3 way ligation - (reply: 2)
352. Query regarding primers for quick change mutagenesis - (reply: 3)
353. What is NEB's CutSmart Buffer? - (reply: 5)
354. Enzyme cutting: low efficiency - (reply: 5)
355. Issue with Xba1 and Dam methylation - (reply: 7)
356. Restriction site rearrangement - (reply: 1)
357. Mutation in cloning - (reply: 6)
358. Must the terminator be in frame? - (reply: 1)
359. sequencing problems - (reply: 2)
360. Understand the basics of molecular cloning - (reply: 6)