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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Problems regarding point mutations.... - (reply: 4)
332. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
333. pkp 59 vector - (reply: 2)
334. Vector insert ratio in cloning - (reply: 3)
335. polymerase to use for cloning - (reply: 4)
336. Oligo insert cloning - (reply: 3)
337. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
338. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
339. How to select competent E. coli cells? - (reply: 7)
340. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
341. cDNA amplification problem - (reply: 4)
342. Ligation: two basic questions - (reply: 1)
343. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
344. Unexpected base after deletion mutagenesis - (reply: 3)
345. Confirmation of ligation - (reply: 7)
346. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
347. Ligation of large ammount of insert (no transformation) - (reply: 7)
348. PCR and restriction enzyme digestion - (reply: 3)
349. Probelm with double digestion - (reply: 1)
350. Cloning not working, all steps checked, clueless - (reply: 1)
351. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
352. recombinant protein form - (reply: 2)
353. restricted PCR plasmid runs slower - (reply: 2)
354. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
355. High frequency of vector religation - (reply: 10)
356. Cloned but not expressing! - (reply: 8)
357. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
358. DNA ligation and trasformation - (reply: 6)
359. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
360. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)