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Top : New Forum Archives (2009-): : Molecular-Cloning
331. cloning help; high salt level in vector - (reply: 4)
332. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
333. problem with gateway cloning: missing part of insert - (reply: 3)
334. T7 and M13 primers two band amplification - (reply: 3)
335. Type of polymerases compatible for TA cloning - (reply: 7)
336. Running undigested plasmids on gel - (reply: 2)
337. Molecular Cloning Sambrook - (reply: 1)
338. What is the membrane made of in DNA purification kits - (reply: 3)
339. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
340. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
341. Removing Introns from a DNA sequence - (reply: 1)
342. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
343. without RE site in PCR product - (reply: 5)
344. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
345. excision of fragment during cloning - (reply: 4)
346. Unusual cloning issue... anyone else?? - (reply: 4)
347. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
348. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
349. How to avoid RNA contamination in DNA samples:? - (reply: 1)
350. Wavy Lines in Gel - (reply: 2)
351. Question Relating to LacZ Disruption - (reply: 4)
352. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
353. Site directed mutagenesis - (reply: 5)
354. PCR insert - in frame? - (reply: 2)
355. Whole plasmid amplification by PCR - (reply: 2)
356. colony PCR after transformation - (reply: 1)
357. For promoter assay, which vector I should use? - (reply: 5)
358. Sequencing of the cloned plasmid - (reply: 5)
359. 4 way cloning into TA Vector - (reply: 10)
360. I get band double the size what does it mean - (reply: 3)