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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
332. How to avoid RNA contamination in DNA samples:? - (reply: 1)
333. Wavy Lines in Gel - (reply: 2)
334. Question Relating to LacZ Disruption - (reply: 4)
335. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
336. Site directed mutagenesis - (reply: 5)
337. PCR insert - in frame? - (reply: 2)
338. Whole plasmid amplification by PCR - (reply: 2)
339. colony PCR after transformation - (reply: 1)
340. For promoter assay, which vector I should use? - (reply: 5)
341. Sequencing of the cloned plasmid - (reply: 5)
342. 4 way cloning into TA Vector - (reply: 10)
343. I get band double the size what does it mean - (reply: 3)
344. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
345. Neeed Help , cloning Confirmation ?! - (reply: 5)
346. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
347. Can I quantify restricted plasmid and oligos? - (reply: 3)
348. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
349. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
350. TOPO TA ligation problems - (reply: 6)
351. Luciferase assay problem - (reply: 2)
352. Double digestion with BamHI and BglII - (reply: 4)
353. nicked linear DNA - (reply: 5)
354. Selection of E.coli competent cells - (reply: 2)
355. Double strand cdna synthysis - (reply: 4)
356. mutagenic primers with very high GC content. - (reply: 3)
357. TA Cloning of ds cDNA - (reply: 6)
358. Promoter distance from ATG - (reply: 3)
359. Measuring Fluorescene of RFP - (reply: 7)
360. Smallest possible insert size for Ligation reaction - (reply: 2)