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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Site directed mutagenesis - (reply: 5)
332. PCR insert - in frame? - (reply: 2)
333. Whole plasmid amplification by PCR - (reply: 2)
334. colony PCR after transformation - (reply: 1)
335. For promoter assay, which vector I should use? - (reply: 5)
336. Sequencing of the cloned plasmid - (reply: 5)
337. 4 way cloning into TA Vector - (reply: 10)
338. I get band double the size what does it mean - (reply: 3)
339. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
340. Neeed Help , cloning Confirmation ?! - (reply: 5)
341. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
342. Can I quantify restricted plasmid and oligos? - (reply: 3)
343. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
344. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
345. TOPO TA ligation problems - (reply: 6)
346. Luciferase assay problem - (reply: 2)
347. Double digestion with BamHI and BglII - (reply: 4)
348. nicked linear DNA - (reply: 5)
349. Selection of E.coli competent cells - (reply: 2)
350. Double strand cdna synthysis - (reply: 4)
351. mutagenic primers with very high GC content. - (reply: 3)
352. TA Cloning of ds cDNA - (reply: 6)
353. Promoter distance from ATG - (reply: 3)
354. Measuring Fluorescene of RFP - (reply: 7)
355. Smallest possible insert size for Ligation reaction - (reply: 2)
356. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
357. Extraction of DNA from termite gut flagellates - (reply: 7)
358. Enzymes and buffers from different companies - (reply: 6)
359. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
360. faint band after plasmid extraction - (reply: 7)