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Top : New Forum Archives (2009-): : Molecular-Cloning
331. TOPO TA ligation problems - (reply: 6)
332. Luciferase assay problem - (reply: 2)
333. Double digestion with BamHI and BglII - (reply: 4)
334. nicked linear DNA - (reply: 5)
335. Selection of E.coli competent cells - (reply: 2)
336. Double strand cdna synthysis - (reply: 4)
337. mutagenic primers with very high GC content. - (reply: 3)
338. TA Cloning of ds cDNA - (reply: 6)
339. Promoter distance from ATG - (reply: 3)
340. Measuring Fluorescene of RFP - (reply: 7)
341. Smallest possible insert size for Ligation reaction - (reply: 2)
342. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
343. Extraction of DNA from termite gut flagellates - (reply: 7)
344. Enzymes and buffers from different companies - (reply: 6)
345. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
346. faint band after plasmid extraction - (reply: 7)
347. Adaptor ligation - (reply: 9)
348. self ligation - (reply: 1)
349. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
350. Cloning large transgenes - (reply: 2)
351. In-fusion HD cloning - (reply: 3)
352. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
353. Few colonies frm sticky end cloning - (reply: 3)
354. transformation problem - (reply: 8)
355. Problems with Digestion? - (reply: 7)
356. plasmid digestion - problem with enzymes? - (reply: 4)
357. Problem with cloning - (reply: 7)
358. double transformation problem - (reply: 3)
359. Too many negative clones using Directional TOPO Cloning - (reply: 5)
360. competent cells - (reply: 1)