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Top : New Forum Archives (2009-): : Molecular-Cloning
331. cloning: band at different site than desired after RE digestion - (reply: 11)
332. GST TAG - (reply: 3)
333. Residues on the N terminal GST Tag - (reply: 1)
334. Where to place the polyA signal? - (reply: 1)
335. identification of target sequence - (reply: 3)
336. cloning pcr insert into plasmid - (reply: 2)
337. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
338. Large quantity heat shock trouble? - (reply: 3)
339. molecular cloning - (reply: 7)
340. Cannot see insert with colony pcr - (reply: 6)
341. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
342. help with a vector - (reply: 3)
343. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
344. Another cloning hell....no transformants - (reply: 1)
345. Agrobacterium LBA4404 transformation - (reply: 1)
346. TOPO TA Cloning Troubleshooting - (reply: 1)
347. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
348. Tricistronic vector - (reply: 1)
349. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
350. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
351. Directions of genes expression illustrations - (reply: 5)
352. Plasmid DNA at RT for a few days - (reply: 4)
353. TOPO TA Cloning - (reply: 1)
354. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
355. No insert in subcloning - (reply: 3)
356. Digest does not confirm presence of fragment - (reply: 5)
357. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
358. pGL3 Promoter replacing TSS bases - (reply: 1)
359. lipofectamine 2000:DNA complex stability - (reply: 2)
360. Problems with Ecoli glycerol stock - (reply: 6)