Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
331. Gel purification (DNA) - (reply: 1)
332. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
333. Cloning and expression problem - (reply: 5)
334. How do silicates adhere nucleic acid? - (reply: 1)
335. Ligation of PCR fragments - (reply: 11)
336. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
337. What plasmid sizes do you work with for SDM? - (reply: 1)
338. cloning help; high salt level in vector - (reply: 4)
339. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
340. problem with gateway cloning: missing part of insert - (reply: 3)
341. T7 and M13 primers two band amplification - (reply: 3)
342. Type of polymerases compatible for TA cloning - (reply: 7)
343. Running undigested plasmids on gel - (reply: 2)
344. Molecular Cloning Sambrook - (reply: 1)
345. What is the membrane made of in DNA purification kits - (reply: 3)
346. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
347. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
348. Removing Introns from a DNA sequence - (reply: 1)
349. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
350. without RE site in PCR product - (reply: 5)
351. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
352. excision of fragment during cloning - (reply: 4)
353. Unusual cloning issue... anyone else?? - (reply: 4)
354. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
355. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
356. How to avoid RNA contamination in DNA samples:? - (reply: 1)
357. Wavy Lines in Gel - (reply: 2)
358. Question Relating to LacZ Disruption - (reply: 4)
359. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
360. Site directed mutagenesis - (reply: 5)