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Top : New Forum Archives (2009-): : Molecular-Cloning
331. For promoter assay, which vector I should use? - (reply: 5)
332. Sequencing of the cloned plasmid - (reply: 5)
333. 4 way cloning into TA Vector - (reply: 10)
334. I get band double the size what does it mean - (reply: 3)
335. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
336. Neeed Help , cloning Confirmation ?! - (reply: 5)
337. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
338. Can I quantify restricted plasmid and oligos? - (reply: 3)
339. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
340. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
341. TOPO TA ligation problems - (reply: 6)
342. Luciferase assay problem - (reply: 2)
343. Double digestion with BamHI and BglII - (reply: 4)
344. nicked linear DNA - (reply: 5)
345. Selection of E.coli competent cells - (reply: 2)
346. Double strand cdna synthysis - (reply: 4)
347. mutagenic primers with very high GC content. - (reply: 3)
348. TA Cloning of ds cDNA - (reply: 6)
349. Promoter distance from ATG - (reply: 3)
350. Measuring Fluorescene of RFP - (reply: 7)
351. Smallest possible insert size for Ligation reaction - (reply: 2)
352. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
353. Extraction of DNA from termite gut flagellates - (reply: 7)
354. Enzymes and buffers from different companies - (reply: 6)
355. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
356. faint band after plasmid extraction - (reply: 7)
357. Adaptor ligation - (reply: 9)
358. self ligation - (reply: 1)
359. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
360. Cloning large transgenes - (reply: 2)