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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Screening for ligation and transformation result - (reply: 2)
332. Problem with 3 way ligation - (reply: 2)
333. Query regarding primers for quick change mutagenesis - (reply: 3)
334. What is NEB's CutSmart Buffer? - (reply: 5)
335. Enzyme cutting: low efficiency - (reply: 5)
336. Issue with Xba1 and Dam methylation - (reply: 7)
337. Restriction site rearrangement - (reply: 1)
338. Mutation in cloning - (reply: 6)
339. Must the terminator be in frame? - (reply: 1)
340. sequencing problems - (reply: 2)
341. Understand the basics of molecular cloning - (reply: 6)
342. Maintainence of transformed cells - (reply: 5)
343. Restriction digest band shift? - (reply: 1)
344. problem in cloning PCR primer design with restriction site - (reply: 4)
345. quick change mutagenesis...... - (reply: 1)
346. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
347. Help for pGEM-T easy vector ligation problem - (reply: 2)
348. Co-transformation - (reply: 2)
349. Problems regarding point mutations.... - (reply: 4)
350. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
351. pkp 59 vector - (reply: 2)
352. Vector insert ratio in cloning - (reply: 3)
353. polymerase to use for cloning - (reply: 4)
354. Oligo insert cloning - (reply: 3)
355. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
356. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
357. How to select competent E. coli cells? - (reply: 7)
358. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
359. cDNA amplification problem - (reply: 4)
360. Ligation: two basic questions - (reply: 1)