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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Molecular Cloning Sambrook - (reply: 1)
332. What is the membrane made of in DNA purification kits - (reply: 3)
333. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
334. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
335. Removing Introns from a DNA sequence - (reply: 1)
336. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
337. without RE site in PCR product - (reply: 5)
338. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
339. excision of fragment during cloning - (reply: 4)
340. Unusual cloning issue... anyone else?? - (reply: 4)
341. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
342. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
343. How to avoid RNA contamination in DNA samples:? - (reply: 1)
344. Wavy Lines in Gel - (reply: 2)
345. Question Relating to LacZ Disruption - (reply: 4)
346. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
347. Site directed mutagenesis - (reply: 5)
348. PCR insert - in frame? - (reply: 2)
349. Whole plasmid amplification by PCR - (reply: 2)
350. colony PCR after transformation - (reply: 1)
351. For promoter assay, which vector I should use? - (reply: 5)
352. Sequencing of the cloned plasmid - (reply: 5)
353. 4 way cloning into TA Vector - (reply: 10)
354. I get band double the size what does it mean - (reply: 3)
355. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
356. Neeed Help , cloning Confirmation ?! - (reply: 5)
357. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
358. Can I quantify restricted plasmid and oligos? - (reply: 3)
359. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
360. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)