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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
332. Removing Introns from a DNA sequence - (reply: 1)
333. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
334. without RE site in PCR product - (reply: 5)
335. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
336. excision of fragment during cloning - (reply: 4)
337. Unusual cloning issue... anyone else?? - (reply: 4)
338. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
339. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
340. How to avoid RNA contamination in DNA samples:? - (reply: 1)
341. Wavy Lines in Gel - (reply: 2)
342. Question Relating to LacZ Disruption - (reply: 4)
343. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
344. Site directed mutagenesis - (reply: 5)
345. PCR insert - in frame? - (reply: 2)
346. Whole plasmid amplification by PCR - (reply: 2)
347. colony PCR after transformation - (reply: 1)
348. For promoter assay, which vector I should use? - (reply: 5)
349. Sequencing of the cloned plasmid - (reply: 5)
350. 4 way cloning into TA Vector - (reply: 10)
351. I get band double the size what does it mean - (reply: 3)
352. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
353. Neeed Help , cloning Confirmation ?! - (reply: 5)
354. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
355. Can I quantify restricted plasmid and oligos? - (reply: 3)
356. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
357. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
358. TOPO TA ligation problems - (reply: 6)
359. Luciferase assay problem - (reply: 2)
360. Double digestion with BamHI and BglII - (reply: 4)