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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Whole plasmid amplification by PCR - (reply: 2)
332. colony PCR after transformation - (reply: 1)
333. For promoter assay, which vector I should use? - (reply: 5)
334. Sequencing of the cloned plasmid - (reply: 5)
335. 4 way cloning into TA Vector - (reply: 10)
336. I get band double the size what does it mean - (reply: 3)
337. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
338. Neeed Help , cloning Confirmation ?! - (reply: 5)
339. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
340. Can I quantify restricted plasmid and oligos? - (reply: 3)
341. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
342. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
343. TOPO TA ligation problems - (reply: 6)
344. Luciferase assay problem - (reply: 2)
345. Double digestion with BamHI and BglII - (reply: 4)
346. nicked linear DNA - (reply: 5)
347. Selection of E.coli competent cells - (reply: 2)
348. Double strand cdna synthysis - (reply: 4)
349. mutagenic primers with very high GC content. - (reply: 3)
350. TA Cloning of ds cDNA - (reply: 6)
351. Promoter distance from ATG - (reply: 3)
352. Measuring Fluorescene of RFP - (reply: 7)
353. Smallest possible insert size for Ligation reaction - (reply: 2)
354. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
355. Extraction of DNA from termite gut flagellates - (reply: 7)
356. Enzymes and buffers from different companies - (reply: 6)
357. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
358. faint band after plasmid extraction - (reply: 7)
359. Adaptor ligation - (reply: 9)
360. self ligation - (reply: 1)