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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Unexpected base after deletion mutagenesis - (reply: 3)
332. Confirmation of ligation - (reply: 7)
333. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
334. Ligation of large ammount of insert (no transformation) - (reply: 7)
335. PCR and restriction enzyme digestion - (reply: 3)
336. Probelm with double digestion - (reply: 1)
337. Cloning not working, all steps checked, clueless - (reply: 1)
338. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
339. recombinant protein form - (reply: 2)
340. restricted PCR plasmid runs slower - (reply: 2)
341. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
342. High frequency of vector religation - (reply: 10)
343. Cloned but not expressing! - (reply: 8)
344. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
345. DNA ligation and trasformation - (reply: 6)
346. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
347. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
348. How to set up simultaneous digestion? - (reply: 3)
349. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
350. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
351. Freeze transformation mixture? - (reply: 2)
352. Cutting my plasmid made it larger - (reply: 7)
353. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
354. Amplification of CAG promoter problems - (reply: 4)
355. restriction enzyme - (reply: 2)
356. Recreate original plasmid by cutting out insert - (reply: 2)
357. Colony-PCR workaround - (reply: 3)
358. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
359. Can I pause a bacterial culture at 4C - (reply: 2)
360. TOPO TA CLONING - LIGATION STEP - (reply: 9)