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Top : New Forum Archives (2009-): : Molecular-Cloning
331. Question Relating to LacZ Disruption - (reply: 4)
332. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
333. Site directed mutagenesis - (reply: 5)
334. PCR insert - in frame? - (reply: 2)
335. Whole plasmid amplification by PCR - (reply: 2)
336. colony PCR after transformation - (reply: 1)
337. For promoter assay, which vector I should use? - (reply: 5)
338. Sequencing of the cloned plasmid - (reply: 5)
339. 4 way cloning into TA Vector - (reply: 10)
340. I get band double the size what does it mean - (reply: 3)
341. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
342. Neeed Help , cloning Confirmation ?! - (reply: 5)
343. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
344. Can I quantify restricted plasmid and oligos? - (reply: 3)
345. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
346. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
347. TOPO TA ligation problems - (reply: 6)
348. Luciferase assay problem - (reply: 2)
349. Double digestion with BamHI and BglII - (reply: 4)
350. nicked linear DNA - (reply: 5)
351. Selection of E.coli competent cells - (reply: 2)
352. Double strand cdna synthysis - (reply: 4)
353. mutagenic primers with very high GC content. - (reply: 3)
354. TA Cloning of ds cDNA - (reply: 6)
355. Promoter distance from ATG - (reply: 3)
356. Measuring Fluorescene of RFP - (reply: 7)
357. Smallest possible insert size for Ligation reaction - (reply: 2)
358. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
359. Extraction of DNA from termite gut flagellates - (reply: 7)
360. Enzymes and buffers from different companies - (reply: 6)