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Top : New Forum Archives (2009-): : Molecular-Cloning
451. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
452. Amplification of CAG promoter problems - (reply: 4)
453. restriction enzyme - (reply: 2)
454. Recreate original plasmid by cutting out insert - (reply: 2)
455. Colony-PCR workaround - (reply: 3)
456. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
457. Can I pause a bacterial culture at 4C - (reply: 2)
458. TOPO TA CLONING - LIGATION STEP - (reply: 9)
459. posting sequences of plasmid? - (reply: 3)
460. Restriction Enzyme Digest not working - (reply: 2)
461. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
462. Incomplete digestion of plasmid with single enzyme - (reply: 1)
463. problem with plasmid digestion - (reply: 5)
464. Difficult Ligation - Details Inside - (reply: 9)
465. pET 32 (+) - (reply: 1)
466. Alternative to pET Vectorsystem - (reply: 2)
467. pEGFP C-1/N-1 Cloning - (reply: 11)
468. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
469. Problems with growing out transformants in liquid LB - (reply: 3)
470. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
471. Ethidiumbromide - (reply: 3)
472. TRYPSIN - (reply: 4)
473. Problem with cloning - what is wrong? - (reply: 6)
474. cloning: band at different site than desired after RE digestion - (reply: 11)
475. GST TAG - (reply: 3)
476. Residues on the N terminal GST Tag - (reply: 1)
477. Where to place the polyA signal? - (reply: 1)
478. identification of target sequence - (reply: 3)
479. cloning pcr insert into plasmid - (reply: 2)
480. TOPO TA CLONING - MOLAR RATIO - (reply: 2)