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Top : New Forum Archives (2009-): : Molecular-Cloning
451. posting sequences of plasmid? - (reply: 3)
452. Restriction Enzyme Digest not working - (reply: 2)
453. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
454. Incomplete digestion of plasmid with single enzyme - (reply: 1)
455. problem with plasmid digestion - (reply: 5)
456. Difficult Ligation - Details Inside - (reply: 9)
457. pET 32 (+) - (reply: 1)
458. Alternative to pET Vectorsystem - (reply: 2)
459. pEGFP C-1/N-1 Cloning - (reply: 11)
460. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
461. Problems with growing out transformants in liquid LB - (reply: 3)
462. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
463. Ethidiumbromide - (reply: 3)
464. TRYPSIN - (reply: 4)
465. Problem with cloning - what is wrong? - (reply: 6)
466. cloning: band at different site than desired after RE digestion - (reply: 11)
467. GST TAG - (reply: 3)
468. Residues on the N terminal GST Tag - (reply: 1)
469. Where to place the polyA signal? - (reply: 1)
470. identification of target sequence - (reply: 3)
471. cloning pcr insert into plasmid - (reply: 2)
472. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
473. Large quantity heat shock trouble? - (reply: 3)
474. molecular cloning - (reply: 7)
475. Cannot see insert with colony pcr - (reply: 6)
476. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
477. help with a vector - (reply: 3)
478. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
479. Another cloning hell....no transformants - (reply: 1)
480. Agrobacterium LBA4404 transformation - (reply: 1)