Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
451. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
452. protein is not expessing in HEK 293 cells - (reply: 2)
453. Restriction problems - (reply: 4)
454. cloning a gene in to a plasmid - (reply: 5)
455. no product on gel after restriction digestion - (reply: 3)
456. Gateway technology. - (reply: 4)
457. Primer concentration for cDNA synthesis using GSP - (reply: 1)
458. cloning from cDNA but got a much shorter PCR product - (reply: 4)
459. GeneArt vs Quikchange - (reply: 1)
460. why do some colonies keep growing in the chiller? - (reply: 7)
461. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
462. Difference between types of bacterial culture broths? - (reply: 4)
463. Co-transfection with 2 plasmids - (reply: 10)
464. Low passage HEK293?? - (reply: 3)
465. Vector Digestion Problem - (reply: 4)
466. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
467. Transformation with a big plasmid - (reply: 4)
468. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
469. Three way ligation problem - (reply: 6)
470. problems with recovery of dna from gel and ligation - (reply: 5)
471. Colony PCR and digestion with KpnI - (reply: 10)
472. Greenish tinge to bacterial culture - (reply: 3)
473. SURE E. coli competent cells - (reply: 1)
474. Ligation - (reply: 20)
475. E. coli growing on plates and bot not in LB medium - (reply: 10)
476. *** Human Insulin promoter sequence*** - (reply: 2)
477. digested plasmid - (reply: 2)
478. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
479. Subcloning - RE digest and ligation and transformation - (reply: 5)
480. Plasmid digested with RE used to transform...what will happen? - (reply: 14)