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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
452. Freeze transformation mixture? - (reply: 2)
453. Cutting my plasmid made it larger - (reply: 7)
454. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
455. Amplification of CAG promoter problems - (reply: 4)
456. restriction enzyme - (reply: 2)
457. Recreate original plasmid by cutting out insert - (reply: 2)
458. Colony-PCR workaround - (reply: 3)
459. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
460. Can I pause a bacterial culture at 4C - (reply: 2)
461. TOPO TA CLONING - LIGATION STEP - (reply: 9)
462. posting sequences of plasmid? - (reply: 3)
463. Restriction Enzyme Digest not working - (reply: 2)
464. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
465. Incomplete digestion of plasmid with single enzyme - (reply: 1)
466. problem with plasmid digestion - (reply: 5)
467. Difficult Ligation - Details Inside - (reply: 9)
468. pET 32 (+) - (reply: 1)
469. Alternative to pET Vectorsystem - (reply: 2)
470. pEGFP C-1/N-1 Cloning - (reply: 11)
471. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
472. Problems with growing out transformants in liquid LB - (reply: 3)
473. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
474. Ethidiumbromide - (reply: 3)
475. TRYPSIN - (reply: 4)
476. Problem with cloning - what is wrong? - (reply: 6)
477. cloning: band at different site than desired after RE digestion - (reply: 11)
478. GST TAG - (reply: 3)
479. Residues on the N terminal GST Tag - (reply: 1)
480. Where to place the polyA signal? - (reply: 1)