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Top : New Forum Archives (2009-): : Molecular-Cloning
451. identification of target sequence - (reply: 3)
452. cloning pcr insert into plasmid - (reply: 2)
453. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
454. Large quantity heat shock trouble? - (reply: 3)
455. molecular cloning - (reply: 7)
456. Cannot see insert with colony pcr - (reply: 6)
457. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
458. help with a vector - (reply: 3)
459. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
460. Another cloning hell....no transformants - (reply: 1)
461. Agrobacterium LBA4404 transformation - (reply: 1)
462. TOPO TA Cloning Troubleshooting - (reply: 1)
463. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
464. Tricistronic vector - (reply: 1)
465. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
466. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
467. Directions of genes expression illustrations - (reply: 5)
468. Plasmid DNA at RT for a few days - (reply: 4)
469. TOPO TA Cloning - (reply: 1)
470. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
471. No insert in subcloning - (reply: 3)
472. Digest does not confirm presence of fragment - (reply: 5)
473. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
474. pGL3 Promoter replacing TSS bases - (reply: 1)
475. lipofectamine 2000:DNA complex stability - (reply: 2)
476. Problems with Ecoli glycerol stock - (reply: 6)
477. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
478. CIAP dilution - (reply: 5)
479. Problems in RNAi construction by using pUC19 - (reply: 3)
480. Unable to clone in E. coli cells - (reply: 3)