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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Ligation of large ammount of insert (no transformation) - (reply: 7)
452. PCR and restriction enzyme digestion - (reply: 3)
453. Probelm with double digestion - (reply: 1)
454. Cloning not working, all steps checked, clueless - (reply: 1)
455. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
456. recombinant protein form - (reply: 2)
457. restricted PCR plasmid runs slower - (reply: 2)
458. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
459. High frequency of vector religation - (reply: 10)
460. Cloned but not expressing! - (reply: 8)
461. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
462. DNA ligation and trasformation - (reply: 6)
463. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
464. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
465. How to set up simultaneous digestion? - (reply: 3)
466. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
467. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
468. Freeze transformation mixture? - (reply: 2)
469. Cutting my plasmid made it larger - (reply: 7)
470. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
471. Amplification of CAG promoter problems - (reply: 4)
472. restriction enzyme - (reply: 2)
473. Recreate original plasmid by cutting out insert - (reply: 2)
474. Colony-PCR workaround - (reply: 3)
475. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
476. Can I pause a bacterial culture at 4C - (reply: 2)
477. TOPO TA CLONING - LIGATION STEP - (reply: 9)
478. posting sequences of plasmid? - (reply: 3)
479. Restriction Enzyme Digest not working - (reply: 2)
480. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)