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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
452. Difference between types of bacterial culture broths? - (reply: 4)
453. Co-transfection with 2 plasmids - (reply: 10)
454. Low passage HEK293?? - (reply: 3)
455. Vector Digestion Problem - (reply: 4)
456. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
457. Transformation with a big plasmid - (reply: 4)
458. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
459. Three way ligation problem - (reply: 6)
460. problems with recovery of dna from gel and ligation - (reply: 5)
461. Colony PCR and digestion with KpnI - (reply: 10)
462. Greenish tinge to bacterial culture - (reply: 3)
463. SURE E. coli competent cells - (reply: 1)
464. Ligation - (reply: 20)
465. E. coli growing on plates and bot not in LB medium - (reply: 10)
466. *** Human Insulin promoter sequence*** - (reply: 2)
467. digested plasmid - (reply: 2)
468. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
469. Subcloning - RE digest and ligation and transformation - (reply: 5)
470. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
471. Making DNA Linkers for cloning purposes - (reply: 1)
472. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
473. Dam-/+ e. coli strains - (reply: 6)
474. cloning short genomic DNA fragments - (reply: 1)
475. finding the protein and nucleotide matches - (reply: 1)
476. DH10B with big construct question - (reply: 2)
477. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
478. cloning to pET28a - (reply: 1)
479. Weird Library Transformation Results - (reply: 10)
480. Injecting mice with lentivirus - (reply: 1)