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Top : New Forum Archives (2009-): : Molecular-Cloning
451. DNA ligation and trasformation - (reply: 6)
452. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
453. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
454. How to set up simultaneous digestion? - (reply: 3)
455. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
456. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
457. Freeze transformation mixture? - (reply: 2)
458. Cutting my plasmid made it larger - (reply: 7)
459. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
460. Amplification of CAG promoter problems - (reply: 4)
461. restriction enzyme - (reply: 2)
462. Recreate original plasmid by cutting out insert - (reply: 2)
463. Colony-PCR workaround - (reply: 3)
464. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
465. Can I pause a bacterial culture at 4C - (reply: 2)
466. TOPO TA CLONING - LIGATION STEP - (reply: 9)
467. posting sequences of plasmid? - (reply: 3)
468. Restriction Enzyme Digest not working - (reply: 2)
469. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
470. Incomplete digestion of plasmid with single enzyme - (reply: 1)
471. problem with plasmid digestion - (reply: 5)
472. Difficult Ligation - Details Inside - (reply: 9)
473. pET 32 (+) - (reply: 1)
474. Alternative to pET Vectorsystem - (reply: 2)
475. pEGFP C-1/N-1 Cloning - (reply: 11)
476. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
477. Problems with growing out transformants in liquid LB - (reply: 3)
478. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
479. Ethidiumbromide - (reply: 3)
480. TRYPSIN - (reply: 4)