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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
452. Unexpected base after deletion mutagenesis - (reply: 3)
453. Confirmation of ligation - (reply: 7)
454. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
455. Ligation of large ammount of insert (no transformation) - (reply: 7)
456. PCR and restriction enzyme digestion - (reply: 3)
457. Probelm with double digestion - (reply: 1)
458. Cloning not working, all steps checked, clueless - (reply: 1)
459. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
460. recombinant protein form - (reply: 2)
461. restricted PCR plasmid runs slower - (reply: 2)
462. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
463. High frequency of vector religation - (reply: 10)
464. Cloned but not expressing! - (reply: 8)
465. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
466. DNA ligation and trasformation - (reply: 6)
467. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
468. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
469. How to set up simultaneous digestion? - (reply: 3)
470. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
471. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
472. Freeze transformation mixture? - (reply: 2)
473. Cutting my plasmid made it larger - (reply: 7)
474. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
475. Amplification of CAG promoter problems - (reply: 4)
476. restriction enzyme - (reply: 2)
477. Recreate original plasmid by cutting out insert - (reply: 2)
478. Colony-PCR workaround - (reply: 3)
479. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
480. Can I pause a bacterial culture at 4C - (reply: 2)