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Top : New Forum Archives (2009-): : Molecular-Cloning
451. cDNA amplification problem - (reply: 4)
452. Ligation: two basic questions - (reply: 1)
453. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
454. Unexpected base after deletion mutagenesis - (reply: 3)
455. Confirmation of ligation - (reply: 7)
456. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
457. Ligation of large ammount of insert (no transformation) - (reply: 7)
458. PCR and restriction enzyme digestion - (reply: 3)
459. Probelm with double digestion - (reply: 1)
460. Cloning not working, all steps checked, clueless - (reply: 1)
461. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
462. recombinant protein form - (reply: 2)
463. restricted PCR plasmid runs slower - (reply: 2)
464. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
465. High frequency of vector religation - (reply: 10)
466. Cloned but not expressing! - (reply: 8)
467. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
468. DNA ligation and trasformation - (reply: 6)
469. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
470. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
471. How to set up simultaneous digestion? - (reply: 3)
472. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
473. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
474. Freeze transformation mixture? - (reply: 2)
475. Cutting my plasmid made it larger - (reply: 7)
476. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
477. Amplification of CAG promoter problems - (reply: 4)
478. restriction enzyme - (reply: 2)
479. Recreate original plasmid by cutting out insert - (reply: 2)
480. Colony-PCR workaround - (reply: 3)