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Top : New Forum Archives (2009-): : Molecular-Cloning
451. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
452. Ligation of large ammount of insert (no transformation) - (reply: 7)
453. PCR and restriction enzyme digestion - (reply: 3)
454. Probelm with double digestion - (reply: 1)
455. Cloning not working, all steps checked, clueless - (reply: 1)
456. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
457. recombinant protein form - (reply: 2)
458. restricted PCR plasmid runs slower - (reply: 2)
459. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
460. High frequency of vector religation - (reply: 10)
461. Cloned but not expressing! - (reply: 8)
462. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
463. DNA ligation and trasformation - (reply: 6)
464. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
465. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
466. How to set up simultaneous digestion? - (reply: 3)
467. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
468. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
469. Freeze transformation mixture? - (reply: 2)
470. Cutting my plasmid made it larger - (reply: 7)
471. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
472. Amplification of CAG promoter problems - (reply: 4)
473. restriction enzyme - (reply: 2)
474. Recreate original plasmid by cutting out insert - (reply: 2)
475. Colony-PCR workaround - (reply: 3)
476. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
477. Can I pause a bacterial culture at 4C - (reply: 2)
478. TOPO TA CLONING - LIGATION STEP - (reply: 9)
479. posting sequences of plasmid? - (reply: 3)
480. Restriction Enzyme Digest not working - (reply: 2)