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Top : New Forum Archives (2009-): : Molecular-Cloning
451. High frequency of vector religation - (reply: 10)
452. Cloned but not expressing! - (reply: 8)
453. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
454. DNA ligation and trasformation - (reply: 6)
455. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
456. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
457. How to set up simultaneous digestion? - (reply: 3)
458. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
459. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
460. Freeze transformation mixture? - (reply: 2)
461. Cutting my plasmid made it larger - (reply: 7)
462. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
463. Amplification of CAG promoter problems - (reply: 4)
464. restriction enzyme - (reply: 2)
465. Recreate original plasmid by cutting out insert - (reply: 2)
466. Colony-PCR workaround - (reply: 3)
467. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
468. Can I pause a bacterial culture at 4C - (reply: 2)
469. TOPO TA CLONING - LIGATION STEP - (reply: 9)
470. posting sequences of plasmid? - (reply: 3)
471. Restriction Enzyme Digest not working - (reply: 2)
472. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
473. Incomplete digestion of plasmid with single enzyme - (reply: 1)
474. problem with plasmid digestion - (reply: 5)
475. Difficult Ligation - Details Inside - (reply: 9)
476. pET 32 (+) - (reply: 1)
477. Alternative to pET Vectorsystem - (reply: 2)
478. pEGFP C-1/N-1 Cloning - (reply: 11)
479. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
480. Problems with growing out transformants in liquid LB - (reply: 3)