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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Oligo insert cloning - (reply: 3)
452. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
453. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
454. How to select competent E. coli cells? - (reply: 7)
455. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
456. cDNA amplification problem - (reply: 4)
457. Ligation: two basic questions - (reply: 1)
458. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
459. Unexpected base after deletion mutagenesis - (reply: 3)
460. Confirmation of ligation - (reply: 7)
461. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
462. Ligation of large ammount of insert (no transformation) - (reply: 7)
463. PCR and restriction enzyme digestion - (reply: 3)
464. Probelm with double digestion - (reply: 1)
465. Cloning not working, all steps checked, clueless - (reply: 1)
466. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
467. recombinant protein form - (reply: 2)
468. restricted PCR plasmid runs slower - (reply: 2)
469. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
470. High frequency of vector religation - (reply: 10)
471. Cloned but not expressing! - (reply: 8)
472. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
473. DNA ligation and trasformation - (reply: 6)
474. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
475. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
476. How to set up simultaneous digestion? - (reply: 3)
477. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
478. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
479. Freeze transformation mixture? - (reply: 2)
480. Cutting my plasmid made it larger - (reply: 7)