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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Linearising plasmid before transfection? - (reply: 2)
452. Smear in plasmid sample - (reply: 9)
453. about a book - (reply: 1)
454. Cloning Problem! I need help - (reply: 3)
455. Help designing primers for use in In-fushion cloning - (reply: 4)
456. Repeat of desired mutation in cloning product - (reply: 1)
457. vector dephosphorylation - (reply: 5)
458. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
459. DH10BAC - (reply: 3)
460. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
461. protein is not expessing in HEK 293 cells - (reply: 2)
462. Restriction problems - (reply: 4)
463. cloning a gene in to a plasmid - (reply: 5)
464. no product on gel after restriction digestion - (reply: 3)
465. Gateway technology. - (reply: 4)
466. Primer concentration for cDNA synthesis using GSP - (reply: 1)
467. cloning from cDNA but got a much shorter PCR product - (reply: 4)
468. GeneArt vs Quikchange - (reply: 1)
469. why do some colonies keep growing in the chiller? - (reply: 7)
470. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
471. Difference between types of bacterial culture broths? - (reply: 4)
472. Co-transfection with 2 plasmids - (reply: 10)
473. Low passage HEK293?? - (reply: 3)
474. Vector Digestion Problem - (reply: 4)
475. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
476. Transformation with a big plasmid - (reply: 4)
477. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
478. Three way ligation problem - (reply: 6)
479. problems with recovery of dna from gel and ligation - (reply: 5)
480. Colony PCR and digestion with KpnI - (reply: 10)