Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
451. Cloned but not expressing! - (reply: 8)
452. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
453. DNA ligation and trasformation - (reply: 6)
454. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
455. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
456. How to set up simultaneous digestion? - (reply: 3)
457. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
458. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
459. Freeze transformation mixture? - (reply: 2)
460. Cutting my plasmid made it larger - (reply: 7)
461. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
462. Amplification of CAG promoter problems - (reply: 4)
463. restriction enzyme - (reply: 2)
464. Recreate original plasmid by cutting out insert - (reply: 2)
465. Colony-PCR workaround - (reply: 3)
466. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
467. Can I pause a bacterial culture at 4C - (reply: 2)
468. TOPO TA CLONING - LIGATION STEP - (reply: 9)
469. posting sequences of plasmid? - (reply: 3)
470. Restriction Enzyme Digest not working - (reply: 2)
471. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
472. Incomplete digestion of plasmid with single enzyme - (reply: 1)
473. problem with plasmid digestion - (reply: 5)
474. Difficult Ligation - Details Inside - (reply: 9)
475. pET 32 (+) - (reply: 1)
476. Alternative to pET Vectorsystem - (reply: 2)
477. pEGFP C-1/N-1 Cloning - (reply: 11)
478. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
479. Problems with growing out transformants in liquid LB - (reply: 3)
480. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)