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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Difficult Ligation - Details Inside - (reply: 9)
452. pET 32 (+) - (reply: 1)
453. Alternative to pET Vectorsystem - (reply: 2)
454. pEGFP C-1/N-1 Cloning - (reply: 11)
455. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
456. Problems with growing out transformants in liquid LB - (reply: 3)
457. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
458. Ethidiumbromide - (reply: 3)
459. TRYPSIN - (reply: 4)
460. Problem with cloning - what is wrong? - (reply: 6)
461. cloning: band at different site than desired after RE digestion - (reply: 11)
462. GST TAG - (reply: 3)
463. Residues on the N terminal GST Tag - (reply: 1)
464. Where to place the polyA signal? - (reply: 1)
465. identification of target sequence - (reply: 3)
466. cloning pcr insert into plasmid - (reply: 2)
467. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
468. Large quantity heat shock trouble? - (reply: 3)
469. molecular cloning - (reply: 7)
470. Cannot see insert with colony pcr - (reply: 6)
471. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
472. help with a vector - (reply: 3)
473. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
474. Another cloning hell....no transformants - (reply: 1)
475. Agrobacterium LBA4404 transformation - (reply: 1)
476. TOPO TA Cloning Troubleshooting - (reply: 1)
477. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
478. Tricistronic vector - (reply: 1)
479. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
480. How would you find a certain tissue specific promoter for my construct? - (reply: 2)