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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
452. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
453. How to select competent E. coli cells? - (reply: 7)
454. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
455. cDNA amplification problem - (reply: 4)
456. Ligation: two basic questions - (reply: 1)
457. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
458. Unexpected base after deletion mutagenesis - (reply: 3)
459. Confirmation of ligation - (reply: 7)
460. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
461. Ligation of large ammount of insert (no transformation) - (reply: 7)
462. PCR and restriction enzyme digestion - (reply: 3)
463. Probelm with double digestion - (reply: 1)
464. Cloning not working, all steps checked, clueless - (reply: 1)
465. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
466. recombinant protein form - (reply: 2)
467. restricted PCR plasmid runs slower - (reply: 2)
468. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
469. High frequency of vector religation - (reply: 10)
470. Cloned but not expressing! - (reply: 8)
471. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
472. DNA ligation and trasformation - (reply: 6)
473. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
474. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
475. How to set up simultaneous digestion? - (reply: 3)
476. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
477. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
478. Freeze transformation mixture? - (reply: 2)
479. Cutting my plasmid made it larger - (reply: 7)
480. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)