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Top : New Forum Archives (2009-): : Molecular-Cloning
451. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
452. How to select competent E. coli cells? - (reply: 7)
453. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
454. cDNA amplification problem - (reply: 4)
455. Ligation: two basic questions - (reply: 1)
456. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
457. Unexpected base after deletion mutagenesis - (reply: 3)
458. Confirmation of ligation - (reply: 7)
459. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
460. Ligation of large ammount of insert (no transformation) - (reply: 7)
461. PCR and restriction enzyme digestion - (reply: 3)
462. Probelm with double digestion - (reply: 1)
463. Cloning not working, all steps checked, clueless - (reply: 1)
464. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
465. recombinant protein form - (reply: 2)
466. restricted PCR plasmid runs slower - (reply: 2)
467. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
468. High frequency of vector religation - (reply: 10)
469. Cloned but not expressing! - (reply: 8)
470. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
471. DNA ligation and trasformation - (reply: 6)
472. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
473. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
474. How to set up simultaneous digestion? - (reply: 3)
475. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
476. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
477. Freeze transformation mixture? - (reply: 2)
478. Cutting my plasmid made it larger - (reply: 7)
479. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
480. Amplification of CAG promoter problems - (reply: 4)