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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Cutting my plasmid made it larger - (reply: 7)
452. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
453. Amplification of CAG promoter problems - (reply: 4)
454. restriction enzyme - (reply: 2)
455. Recreate original plasmid by cutting out insert - (reply: 2)
456. Colony-PCR workaround - (reply: 3)
457. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
458. Can I pause a bacterial culture at 4C - (reply: 2)
459. TOPO TA CLONING - LIGATION STEP - (reply: 9)
460. posting sequences of plasmid? - (reply: 3)
461. Restriction Enzyme Digest not working - (reply: 2)
462. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
463. Incomplete digestion of plasmid with single enzyme - (reply: 1)
464. problem with plasmid digestion - (reply: 5)
465. Difficult Ligation - Details Inside - (reply: 9)
466. pET 32 (+) - (reply: 1)
467. Alternative to pET Vectorsystem - (reply: 2)
468. pEGFP C-1/N-1 Cloning - (reply: 11)
469. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
470. Problems with growing out transformants in liquid LB - (reply: 3)
471. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
472. Ethidiumbromide - (reply: 3)
473. TRYPSIN - (reply: 4)
474. Problem with cloning - what is wrong? - (reply: 6)
475. cloning: band at different site than desired after RE digestion - (reply: 11)
476. GST TAG - (reply: 3)
477. Residues on the N terminal GST Tag - (reply: 1)
478. Where to place the polyA signal? - (reply: 1)
479. identification of target sequence - (reply: 3)
480. cloning pcr insert into plasmid - (reply: 2)