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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Large quantity heat shock trouble? - (reply: 3)
452. molecular cloning - (reply: 7)
453. Cannot see insert with colony pcr - (reply: 6)
454. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
455. help with a vector - (reply: 3)
456. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
457. Another cloning hell....no transformants - (reply: 1)
458. Agrobacterium LBA4404 transformation - (reply: 1)
459. TOPO TA Cloning Troubleshooting - (reply: 1)
460. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
461. Tricistronic vector - (reply: 1)
462. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
463. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
464. Directions of genes expression illustrations - (reply: 5)
465. Plasmid DNA at RT for a few days - (reply: 4)
466. TOPO TA Cloning - (reply: 1)
467. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
468. No insert in subcloning - (reply: 3)
469. Digest does not confirm presence of fragment - (reply: 5)
470. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
471. pGL3 Promoter replacing TSS bases - (reply: 1)
472. lipofectamine 2000:DNA complex stability - (reply: 2)
473. Problems with Ecoli glycerol stock - (reply: 6)
474. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
475. CIAP dilution - (reply: 5)
476. Problems in RNAi construction by using pUC19 - (reply: 3)
477. Unable to clone in E. coli cells - (reply: 3)
478. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
479. plasmid rearrangement or sabotage - (reply: 2)
480. pLDR20 Vector - (reply: 1)