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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
452. How to set up simultaneous digestion? - (reply: 3)
453. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
454. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
455. Freeze transformation mixture? - (reply: 2)
456. Cutting my plasmid made it larger - (reply: 7)
457. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
458. Amplification of CAG promoter problems - (reply: 4)
459. restriction enzyme - (reply: 2)
460. Recreate original plasmid by cutting out insert - (reply: 2)
461. Colony-PCR workaround - (reply: 3)
462. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
463. Can I pause a bacterial culture at 4C - (reply: 2)
464. TOPO TA CLONING - LIGATION STEP - (reply: 9)
465. posting sequences of plasmid? - (reply: 3)
466. Restriction Enzyme Digest not working - (reply: 2)
467. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
468. Incomplete digestion of plasmid with single enzyme - (reply: 1)
469. problem with plasmid digestion - (reply: 5)
470. Difficult Ligation - Details Inside - (reply: 9)
471. pET 32 (+) - (reply: 1)
472. Alternative to pET Vectorsystem - (reply: 2)
473. pEGFP C-1/N-1 Cloning - (reply: 11)
474. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
475. Problems with growing out transformants in liquid LB - (reply: 3)
476. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
477. Ethidiumbromide - (reply: 3)
478. TRYPSIN - (reply: 4)
479. Problem with cloning - what is wrong? - (reply: 6)
480. cloning: band at different site than desired after RE digestion - (reply: 11)