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Top : New Forum Archives (2009-): : Molecular-Cloning
451. Freeze transformation mixture? - (reply: 2)
452. Cutting my plasmid made it larger - (reply: 7)
453. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
454. Amplification of CAG promoter problems - (reply: 4)
455. restriction enzyme - (reply: 2)
456. Recreate original plasmid by cutting out insert - (reply: 2)
457. Colony-PCR workaround - (reply: 3)
458. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
459. Can I pause a bacterial culture at 4C - (reply: 2)
460. TOPO TA CLONING - LIGATION STEP - (reply: 9)
461. posting sequences of plasmid? - (reply: 3)
462. Restriction Enzyme Digest not working - (reply: 2)
463. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
464. Incomplete digestion of plasmid with single enzyme - (reply: 1)
465. problem with plasmid digestion - (reply: 5)
466. Difficult Ligation - Details Inside - (reply: 9)
467. pET 32 (+) - (reply: 1)
468. Alternative to pET Vectorsystem - (reply: 2)
469. pEGFP C-1/N-1 Cloning - (reply: 11)
470. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
471. Problems with growing out transformants in liquid LB - (reply: 3)
472. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
473. Ethidiumbromide - (reply: 3)
474. TRYPSIN - (reply: 4)
475. Problem with cloning - what is wrong? - (reply: 6)
476. cloning: band at different site than desired after RE digestion - (reply: 11)
477. GST TAG - (reply: 3)
478. Residues on the N terminal GST Tag - (reply: 1)
479. Where to place the polyA signal? - (reply: 1)
480. identification of target sequence - (reply: 3)