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Top : New Forum Archives (2009-): : Molecular-Cloning
451. How to select competent E. coli cells? - (reply: 7)
452. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
453. cDNA amplification problem - (reply: 4)
454. Ligation: two basic questions - (reply: 1)
455. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
456. Unexpected base after deletion mutagenesis - (reply: 3)
457. Confirmation of ligation - (reply: 7)
458. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
459. Ligation of large ammount of insert (no transformation) - (reply: 7)
460. PCR and restriction enzyme digestion - (reply: 3)
461. Probelm with double digestion - (reply: 1)
462. Cloning not working, all steps checked, clueless - (reply: 1)
463. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
464. recombinant protein form - (reply: 2)
465. restricted PCR plasmid runs slower - (reply: 2)
466. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
467. High frequency of vector religation - (reply: 10)
468. Cloned but not expressing! - (reply: 8)
469. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
470. DNA ligation and trasformation - (reply: 6)
471. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
472. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
473. How to set up simultaneous digestion? - (reply: 3)
474. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
475. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
476. Freeze transformation mixture? - (reply: 2)
477. Cutting my plasmid made it larger - (reply: 7)
478. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
479. Amplification of CAG promoter problems - (reply: 4)
480. restriction enzyme - (reply: 2)