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Top : New Forum Archives (2009-): : Molecular-Cloning
1561. How to clean up restriction enzyme digestion - (reply: 8)
1562. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1563. GPCR - (reply: 1)
1564. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1565. finding the suitable RE - first timer (reply: 6)
1566. TOPO TA Problems - TOPO TA Problems (reply: 8)
1567. about antibiotic addition - (reply: 4)
1568. about chloramphenicol and L.lactis - (reply: 1)
1569. ligation - (reply: 5)
1570. Losing insert from pGEM? - (reply: 2)
1571. How to concentrate lenti or retroviral particules - (reply: 3)
1572. wierd clot in LB culture - (reply: 5)
1573. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1574. Not getting enough colony - (reply: 4)
1575. Ligation problem - (reply: 9)
1576. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1577. about flanking sequnce - (reply: 3)
1578. Ligation issues - (reply: 4)
1579. messed up restriction sites - (reply: 3)
1580. annoying PCR cloning problem (season 2) - (reply: 5)
1581. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
1582. size limit of plasmid origins - (reply: 2)
1583. Problems with Stratagene Site Directed Mutagenesis - (reply: 36)
1584. confusion about 5'UTR - confusion about 5'UTR (reply: 1)
1585. qPCR - urgent help (reply: 2)
1586. GFP - (reply: 2)
1587. Creating empty vector by self-ligation - (reply: 31)
1588. ligation probs - (reply: 2)
1589. Insert phosphorylation upon Klenow? - (reply: 5)
1590. confusion with different forms of plasmid - (reply: 3)