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Top : New Forum Archives (2009-): : Molecular-Cloning
1561. Touchdown PCR - (reply: 1)
1562. Adding A tails? - (reply: 3)
1563. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1564. Determine annealing temperature of primers - (reply: 14)
1565. Bacteria pellet has almost-black region - (reply: 10)
1566. no ligation !!!!! - (reply: 6)
1567. How to clean up restriction enzyme digestion - (reply: 8)
1568. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1569. GPCR - (reply: 1)
1570. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1571. finding the suitable RE - first timer (reply: 6)
1572. TOPO TA Problems - TOPO TA Problems (reply: 8)
1573. about antibiotic addition - (reply: 4)
1574. about chloramphenicol and L.lactis - (reply: 1)
1575. ligation - (reply: 5)
1576. Losing insert from pGEM? - (reply: 2)
1577. How to concentrate lenti or retroviral particules - (reply: 3)
1578. wierd clot in LB culture - (reply: 5)
1579. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1580. Not getting enough colony - (reply: 4)
1581. Ligation problem - (reply: 9)
1582. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1583. about flanking sequnce - (reply: 3)
1584. Ligation issues - (reply: 4)
1585. messed up restriction sites - (reply: 3)
1586. annoying PCR cloning problem (season 2) - (reply: 5)
1587. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
1588. size limit of plasmid origins - (reply: 2)
1589. Problems with Stratagene Site Directed Mutagenesis - (reply: 36)
1590. confusion about 5'UTR - confusion about 5'UTR (reply: 1)