Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1561. Determine annealing temperature of primers - (reply: 14)
1562. Bacteria pellet has almost-black region - (reply: 10)
1563. no ligation !!!!! - (reply: 6)
1564. How to clean up restriction enzyme digestion - (reply: 8)
1565. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1566. GPCR - (reply: 1)
1567. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1568. finding the suitable RE - first timer (reply: 6)
1569. TOPO TA Problems - TOPO TA Problems (reply: 8)
1570. about antibiotic addition - (reply: 4)
1571. about chloramphenicol and L.lactis - (reply: 1)
1572. ligation - (reply: 5)
1573. Losing insert from pGEM? - (reply: 2)
1574. How to concentrate lenti or retroviral particules - (reply: 3)
1575. wierd clot in LB culture - (reply: 5)
1576. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1577. Not getting enough colony - (reply: 4)
1578. Ligation problem - (reply: 9)
1579. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1580. about flanking sequnce - (reply: 3)
1581. Ligation issues - (reply: 4)
1582. messed up restriction sites - (reply: 3)
1583. annoying PCR cloning problem (season 2) - (reply: 5)
1584. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
1585. size limit of plasmid origins - (reply: 2)
1586. Problems with Stratagene Site Directed Mutagenesis - (reply: 36)
1587. confusion about 5'UTR - confusion about 5'UTR (reply: 1)
1588. qPCR - urgent help (reply: 2)
1589. GFP - (reply: 2)
1590. Creating empty vector by self-ligation - (reply: 31)