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Top : New Forum Archives (2009-): : Molecular-Cloning
961. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
962. rna isolation - (reply: 1)
963. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
964. So many colonies! - (reply: 8)
965. Large vector cloning-recombination problem? - (reply: 1)
966. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
967. selecting recombinants - (reply: 1)
968. colony PCR for subcloning gene - (reply: 1)
969. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
970. cloning of a 20 kb insert - (reply: 2)
971. DNA quantification after gel purification - (reply: 6)
972. cloning of an operon - (reply: 2)
973. storing plasmid - -80degrees (reply: 4)
974. verifying a positive transformant - (reply: 3)
975. Need advice on cloning very small insert into a vector - (reply: 3)
976. Cloning problems i.e. Ligation - (reply: 2)
977. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
978. Radioactive carbon - (reply: 3)
979. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
980. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
981. Digesting large amount of plasmid - (reply: 4)
982. cleanup of digestion before ligation - (reply: 6)
983. transformation efficiency - (reply: 7)
984. transformed bacteria doesnt grow - (reply: 1)
985. TOPO Cloning - (reply: 5)
986. blunt ligation - (reply: 4)
987. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
988. About Laminin 5'UTR 's function - (reply: 1)
989. Vectors with special MCS - (reply: 5)
990. Cloning <100 bp fragments into a vector - (reply: 7)