Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
961. Really strange problem with sequencing and plasmid purification! - (reply: 8)
962. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
963. what is site-specific integration - (reply: 2)
964. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
965. double transformations - (reply: 1)
966. Unusual band in transformants - (reply: 2)
967. ligation--pls help! - (reply: 10)
968. bizarre digestion:) - (reply: 2)
969. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
970. metagenomic library consturction - BAC vectors (reply: 1)
971. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
972. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
973. Chromosomal recombination with BL21? - (reply: 2)
974. Restriction Digestion!! - (reply: 5)
975. digestion - restriction enzymes (reply: 2)
976. How to prevent self ligation - selfligation (reply: 11)
977. what is the difference between DH10B and Top10 - (reply: 1)
978. problems encountered when working on a large plasmid - (reply: 2)
979. make a Mega plasmid mutation - (reply: 1)
980. Cloning - (reply: 1)
981. Copy-number calculation formula? - (reply: 1)
982. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
983. low melt agarose - (reply: 1)
984. restriction digest - smear - (reply: 1)
985. designing of primers - (reply: 2)
986. Propagating ccdB survival cells - (reply: 6)
987. Bicistronic vector vs "normal" vector - (reply: 5)
988. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
989. Can't seem to get insert into vector... please help - (reply: 4)
990. plasmid cloning - i need help.. can anyone answer me (reply: 5)