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Top : New Forum Archives (2009-): : Molecular-Cloning
961. ligating small inert into a large vector - (reply: 4)
962. tranformation trouble using dh5alpha - (reply: 1)
963. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
964. Starting cells for making one's own competent cells - (reply: 7)
965. 6 months... and still no positive clones! - (reply: 4)
966. Really strange problem with sequencing and plasmid purification! - (reply: 8)
967. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
968. what is site-specific integration - (reply: 2)
969. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
970. double transformations - (reply: 1)
971. Unusual band in transformants - (reply: 2)
972. ligation--pls help! - (reply: 10)
973. bizarre digestion:) - (reply: 2)
974. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
975. metagenomic library consturction - BAC vectors (reply: 1)
976. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
977. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
978. Chromosomal recombination with BL21? - (reply: 2)
979. Restriction Digestion!! - (reply: 5)
980. digestion - restriction enzymes (reply: 2)
981. How to prevent self ligation - selfligation (reply: 11)
982. what is the difference between DH10B and Top10 - (reply: 1)
983. problems encountered when working on a large plasmid - (reply: 2)
984. make a Mega plasmid mutation - (reply: 1)
985. Cloning - (reply: 1)
986. Copy-number calculation formula? - (reply: 1)
987. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
988. low melt agarose - (reply: 1)
989. restriction digest - smear - (reply: 1)
990. designing of primers - (reply: 2)