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Top : New Forum Archives (2009-): : Molecular-Cloning
961. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
962. what is site-specific integration - (reply: 2)
963. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
964. double transformations - (reply: 1)
965. Unusual band in transformants - (reply: 2)
966. ligation--pls help! - (reply: 10)
967. bizarre digestion:) - (reply: 2)
968. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
969. metagenomic library consturction - BAC vectors (reply: 1)
970. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
971. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
972. Chromosomal recombination with BL21? - (reply: 2)
973. Restriction Digestion!! - (reply: 5)
974. digestion - restriction enzymes (reply: 2)
975. How to prevent self ligation - selfligation (reply: 11)
976. what is the difference between DH10B and Top10 - (reply: 1)
977. problems encountered when working on a large plasmid - (reply: 2)
978. make a Mega plasmid mutation - (reply: 1)
979. Cloning - (reply: 1)
980. Copy-number calculation formula? - (reply: 1)
981. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
982. low melt agarose - (reply: 1)
983. restriction digest - smear - (reply: 1)
984. designing of primers - (reply: 2)
985. Propagating ccdB survival cells - (reply: 6)
986. Bicistronic vector vs "normal" vector - (reply: 5)
987. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
988. Can't seem to get insert into vector... please help - (reply: 4)
989. plasmid cloning - i need help.. can anyone answer me (reply: 5)
990. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)