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Top : New Forum Archives (2009-): : Molecular-Cloning
961. storing plasmid - -80degrees (reply: 4)
962. verifying a positive transformant - (reply: 3)
963. Need advice on cloning very small insert into a vector - (reply: 3)
964. Cloning problems i.e. Ligation - (reply: 2)
965. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
966. Radioactive carbon - (reply: 3)
967. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
968. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
969. Digesting large amount of plasmid - (reply: 4)
970. cleanup of digestion before ligation - (reply: 6)
971. transformation efficiency - (reply: 7)
972. transformed bacteria doesnt grow - (reply: 1)
973. TOPO Cloning - (reply: 5)
974. blunt ligation - (reply: 4)
975. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
976. About Laminin 5'UTR 's function - (reply: 1)
977. Vectors with special MCS - (reply: 5)
978. Cloning <100 bp fragments into a vector - (reply: 7)
979. Help! my plasmid size changed after ligation - (reply: 5)
980. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
981. Restriction digestion of gDNA for phage library - (reply: 5)
982. how i can get insert back? - (reply: 1)
983. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
984. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
985. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
986. mammalian expression vector - (reply: 2)
987. Electroporation doesn't like my plasmid??? - (reply: 4)
988. low yeild fo RNA after invitro transcription - (reply: 4)
989. No expresion of transgene - Retroviral vector (reply: 1)
990. plasmids - plasmids for stable cell line construction (reply: 2)