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Top : New Forum Archives (2009-): : Molecular-Cloning
961. cloning help - (reply: 3)
962. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
963. A gene is not inserted into the Plasmid - (reply: 2)
964. Air bubbles during electroporation - (reply: 1)
965. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
966. Colonies without inserts - (reply: 9)
967. Transformants Don't Grow - (reply: 4)
968. control DNA transformation- using pGEM kit - transformation (reply: 4)
969. Topo Cloning - Understanding the principle (reply: 6)
970. pMX-PURO Vector not growing - (reply: 3)
971. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
972. how to set up the ligation - (reply: 14)
973. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
974. DH5 Alpha Transformation - (reply: 2)
975. Cloning a foreign gene into E-coli - (reply: 1)
976. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
977. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
978. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
979. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
980. restriction digest simple question - (reply: 4)
981. ligating small inert into a large vector - (reply: 4)
982. tranformation trouble using dh5alpha - (reply: 1)
983. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
984. Starting cells for making one's own competent cells - (reply: 7)
985. 6 months... and still no positive clones! - (reply: 4)
986. Really strange problem with sequencing and plasmid purification! - (reply: 8)
987. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
988. what is site-specific integration - (reply: 2)
989. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
990. double transformations - (reply: 1)