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Top : New Forum Archives (2009-): : Molecular-Cloning
961. 6 months... and still no positive clones! - (reply: 4)
962. Really strange problem with sequencing and plasmid purification! - (reply: 8)
963. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
964. what is site-specific integration - (reply: 2)
965. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
966. double transformations - (reply: 1)
967. Unusual band in transformants - (reply: 2)
968. ligation--pls help! - (reply: 10)
969. bizarre digestion:) - (reply: 2)
970. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
971. metagenomic library consturction - BAC vectors (reply: 1)
972. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
973. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
974. Chromosomal recombination with BL21? - (reply: 2)
975. Restriction Digestion!! - (reply: 5)
976. digestion - restriction enzymes (reply: 2)
977. How to prevent self ligation - selfligation (reply: 11)
978. what is the difference between DH10B and Top10 - (reply: 1)
979. problems encountered when working on a large plasmid - (reply: 2)
980. make a Mega plasmid mutation - (reply: 1)
981. Cloning - (reply: 1)
982. Copy-number calculation formula? - (reply: 1)
983. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
984. low melt agarose - (reply: 1)
985. restriction digest - smear - (reply: 1)
986. designing of primers - (reply: 2)
987. Propagating ccdB survival cells - (reply: 6)
988. Bicistronic vector vs "normal" vector - (reply: 5)
989. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
990. Can't seem to get insert into vector... please help - (reply: 4)