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Top : New Forum Archives (2009-): : Molecular-Cloning
961. Topo cloning or pGEM-T easy ? - (reply: 3)
962. Problems with qiaquick gel purification kit - cloning (reply: 10)
963. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
964. integration of a gene per homologous recombination - (reply: 5)
965. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
966. Antarctic Phosphatase treatment - (reply: 5)
967. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
968. phage DNA - DNA wont migrate on agarose gel (reply: 3)
969. Plasmid out of BL21 for mutagensis - (reply: 3)
970. no colony growth - (reply: 4)
971. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
972. Effect of a DNA prep on DE3 - (reply: 2)
973. Competent cell storage for long term use - (reply: 2)
974. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
975. New to sequencing, primer design - (reply: 2)
976. ta cloning problem - (reply: 2)
977. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
978. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
979. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
980. Storage of Bacterial Culture at 4 degrees - (reply: 2)
981. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
982. rna isolation - (reply: 1)
983. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
984. Molecular Cloning od Recombinant DNA - (reply: 3)
985. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
986. looking for binding site - software? (reply: 1)
987. Insertion of loxp/avoiding SINE - (reply: 1)
988. Question: Ligation Problem.... - (reply: 2)
989. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
990. Topo directional cloning - pENTR/D-TOPO (reply: 1)