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Top : New Forum Archives (2009-): : Molecular-Cloning
961. Can't seem to get insert into vector... please help - (reply: 4)
962. plasmid cloning - i need help.. can anyone answer me (reply: 5)
963. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
964. Topo cloning or pGEM-T easy ? - (reply: 3)
965. Problems with qiaquick gel purification kit - cloning (reply: 10)
966. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
967. integration of a gene per homologous recombination - (reply: 5)
968. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
969. Antarctic Phosphatase treatment - (reply: 5)
970. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
971. phage DNA - DNA wont migrate on agarose gel (reply: 3)
972. Plasmid out of BL21 for mutagensis - (reply: 3)
973. no colony growth - (reply: 4)
974. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
975. Effect of a DNA prep on DE3 - (reply: 2)
976. Competent cell storage for long term use - (reply: 2)
977. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
978. New to sequencing, primer design - (reply: 2)
979. ta cloning problem - (reply: 2)
980. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
981. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
982. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
983. Storage of Bacterial Culture at 4 degrees - (reply: 2)
984. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
985. rna isolation - (reply: 1)
986. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
987. Molecular Cloning od Recombinant DNA - (reply: 3)
988. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
989. looking for binding site - software? (reply: 1)
990. Insertion of loxp/avoiding SINE - (reply: 1)