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Top : New Forum Archives (2009-): : Molecular-Cloning
961. Starting cells for making one's own competent cells - (reply: 7)
962. 6 months... and still no positive clones! - (reply: 4)
963. Really strange problem with sequencing and plasmid purification! - (reply: 8)
964. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
965. what is site-specific integration - (reply: 2)
966. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
967. double transformations - (reply: 1)
968. Unusual band in transformants - (reply: 2)
969. ligation--pls help! - (reply: 10)
970. bizarre digestion:) - (reply: 2)
971. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
972. metagenomic library consturction - BAC vectors (reply: 1)
973. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
974. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
975. Chromosomal recombination with BL21? - (reply: 2)
976. Restriction Digestion!! - (reply: 5)
977. digestion - restriction enzymes (reply: 2)
978. How to prevent self ligation - selfligation (reply: 11)
979. what is the difference between DH10B and Top10 - (reply: 1)
980. problems encountered when working on a large plasmid - (reply: 2)
981. make a Mega plasmid mutation - (reply: 1)
982. Cloning - (reply: 1)
983. Copy-number calculation formula? - (reply: 1)
984. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
985. low melt agarose - (reply: 1)
986. restriction digest - smear - (reply: 1)
987. designing of primers - (reply: 2)
988. Propagating ccdB survival cells - (reply: 6)
989. Bicistronic vector vs "normal" vector - (reply: 5)
990. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)