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Top : New Forum Archives (2009-): : Molecular-Cloning
961. A gene is not inserted into the Plasmid - (reply: 2)
962. Air bubbles during electroporation - (reply: 1)
963. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
964. Colonies without inserts - (reply: 9)
965. Transformants Don't Grow - (reply: 4)
966. control DNA transformation- using pGEM kit - transformation (reply: 4)
967. Topo Cloning - Understanding the principle (reply: 6)
968. pMX-PURO Vector not growing - (reply: 3)
969. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
970. how to set up the ligation - (reply: 14)
971. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
972. DH5 Alpha Transformation - (reply: 2)
973. Cloning a foreign gene into E-coli - (reply: 1)
974. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
975. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
976. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
977. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
978. restriction digest simple question - (reply: 4)
979. ligating small inert into a large vector - (reply: 4)
980. tranformation trouble using dh5alpha - (reply: 1)
981. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
982. Starting cells for making one's own competent cells - (reply: 7)
983. 6 months... and still no positive clones! - (reply: 4)
984. Really strange problem with sequencing and plasmid purification! - (reply: 8)
985. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
986. what is site-specific integration - (reply: 2)
987. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
988. double transformations - (reply: 1)
989. Unusual band in transformants - (reply: 2)
990. ligation--pls help! - (reply: 10)