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Top : New Forum Archives (2009-): : Molecular-Cloning
961. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
962. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
963. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
964. restriction digest simple question - (reply: 4)
965. ligating small inert into a large vector - (reply: 4)
966. tranformation trouble using dh5alpha - (reply: 1)
967. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
968. Starting cells for making one's own competent cells - (reply: 7)
969. 6 months... and still no positive clones! - (reply: 4)
970. Really strange problem with sequencing and plasmid purification! - (reply: 8)
971. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
972. what is site-specific integration - (reply: 2)
973. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
974. double transformations - (reply: 1)
975. Unusual band in transformants - (reply: 2)
976. ligation--pls help! - (reply: 10)
977. bizarre digestion:) - (reply: 2)
978. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
979. metagenomic library consturction - BAC vectors (reply: 1)
980. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
981. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
982. Chromosomal recombination with BL21? - (reply: 2)
983. Restriction Digestion!! - (reply: 5)
984. digestion - restriction enzymes (reply: 2)
985. How to prevent self ligation - selfligation (reply: 11)
986. what is the difference between DH10B and Top10 - (reply: 1)
987. problems encountered when working on a large plasmid - (reply: 2)
988. make a Mega plasmid mutation - (reply: 1)
989. Cloning - (reply: 1)
990. Copy-number calculation formula? - (reply: 1)