Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
961. Cloning a foreign gene into E-coli - (reply: 1)
962. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
963. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
964. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
965. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
966. restriction digest simple question - (reply: 4)
967. ligating small inert into a large vector - (reply: 4)
968. tranformation trouble using dh5alpha - (reply: 1)
969. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
970. Starting cells for making one's own competent cells - (reply: 7)
971. 6 months... and still no positive clones! - (reply: 4)
972. Really strange problem with sequencing and plasmid purification! - (reply: 8)
973. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
974. what is site-specific integration - (reply: 2)
975. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
976. double transformations - (reply: 1)
977. Unusual band in transformants - (reply: 2)
978. ligation--pls help! - (reply: 10)
979. bizarre digestion:) - (reply: 2)
980. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
981. metagenomic library consturction - BAC vectors (reply: 1)
982. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
983. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
984. Chromosomal recombination with BL21? - (reply: 2)
985. Restriction Digestion!! - (reply: 5)
986. digestion - restriction enzymes (reply: 2)
987. How to prevent self ligation - selfligation (reply: 11)
988. what is the difference between DH10B and Top10 - (reply: 1)
989. problems encountered when working on a large plasmid - (reply: 2)
990. make a Mega plasmid mutation - (reply: 1)