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Top : New Forum Archives (2009-): : Molecular-Cloning
961. Transformants Don't Grow - (reply: 4)
962. control DNA transformation- using pGEM kit - transformation (reply: 4)
963. Topo Cloning - Understanding the principle (reply: 6)
964. pMX-PURO Vector not growing - (reply: 3)
965. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
966. how to set up the ligation - (reply: 14)
967. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
968. DH5 Alpha Transformation - (reply: 2)
969. Cloning a foreign gene into E-coli - (reply: 1)
970. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
971. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
972. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
973. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
974. restriction digest simple question - (reply: 4)
975. ligating small inert into a large vector - (reply: 4)
976. tranformation trouble using dh5alpha - (reply: 1)
977. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
978. Starting cells for making one's own competent cells - (reply: 7)
979. 6 months... and still no positive clones! - (reply: 4)
980. Really strange problem with sequencing and plasmid purification! - (reply: 8)
981. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
982. what is site-specific integration - (reply: 2)
983. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
984. double transformations - (reply: 1)
985. Unusual band in transformants - (reply: 2)
986. ligation--pls help! - (reply: 10)
987. bizarre digestion:) - (reply: 2)
988. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
989. metagenomic library consturction - BAC vectors (reply: 1)
990. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)