Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
961. Colonies without inserts - (reply: 9)
962. Transformants Don't Grow - (reply: 4)
963. control DNA transformation- using pGEM kit - transformation (reply: 4)
964. Topo Cloning - Understanding the principle (reply: 6)
965. pMX-PURO Vector not growing - (reply: 3)
966. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
967. how to set up the ligation - (reply: 14)
968. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
969. DH5 Alpha Transformation - (reply: 2)
970. Cloning a foreign gene into E-coli - (reply: 1)
971. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
972. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
973. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
974. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
975. restriction digest simple question - (reply: 4)
976. ligating small inert into a large vector - (reply: 4)
977. tranformation trouble using dh5alpha - (reply: 1)
978. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
979. Starting cells for making one's own competent cells - (reply: 7)
980. 6 months... and still no positive clones! - (reply: 4)
981. Really strange problem with sequencing and plasmid purification! - (reply: 8)
982. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
983. what is site-specific integration - (reply: 2)
984. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
985. double transformations - (reply: 1)
986. Unusual band in transformants - (reply: 2)
987. ligation--pls help! - (reply: 10)
988. bizarre digestion:) - (reply: 2)
989. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
990. metagenomic library consturction - BAC vectors (reply: 1)