Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
541. Difference between types of bacterial culture broths? - (reply: 4)
542. Co-transfection with 2 plasmids - (reply: 10)
543. Low passage HEK293?? - (reply: 3)
544. Vector Digestion Problem - (reply: 4)
545. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
546. Transformation with a big plasmid - (reply: 4)
547. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
548. Three way ligation problem - (reply: 6)
549. problems with recovery of dna from gel and ligation - (reply: 5)
550. Colony PCR and digestion with KpnI - (reply: 10)
551. Greenish tinge to bacterial culture - (reply: 3)
552. SURE E. coli competent cells - (reply: 1)
553. Ligation - (reply: 20)
554. E. coli growing on plates and bot not in LB medium - (reply: 10)
555. *** Human Insulin promoter sequence*** - (reply: 2)
556. digested plasmid - (reply: 2)
557. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
558. Subcloning - RE digest and ligation and transformation - (reply: 5)
559. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
560. Making DNA Linkers for cloning purposes - (reply: 1)
561. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
562. Dam-/+ e. coli strains - (reply: 6)
563. cloning short genomic DNA fragments - (reply: 1)
564. finding the protein and nucleotide matches - (reply: 1)
565. DH10B with big construct question - (reply: 2)
566. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
567. cloning to pET28a - (reply: 1)
568. Weird Library Transformation Results - (reply: 10)
569. Injecting mice with lentivirus - (reply: 1)
570. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)