Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
541. GeneArt vs Quikchange - (reply: 1)
542. why do some colonies keep growing in the chiller? - (reply: 7)
543. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
544. Difference between types of bacterial culture broths? - (reply: 4)
545. Co-transfection with 2 plasmids - (reply: 10)
546. Low passage HEK293?? - (reply: 3)
547. Vector Digestion Problem - (reply: 4)
548. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
549. Transformation with a big plasmid - (reply: 4)
550. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
551. Three way ligation problem - (reply: 6)
552. problems with recovery of dna from gel and ligation - (reply: 5)
553. Colony PCR and digestion with KpnI - (reply: 10)
554. Greenish tinge to bacterial culture - (reply: 3)
555. SURE E. coli competent cells - (reply: 1)
556. Ligation - (reply: 20)
557. E. coli growing on plates and bot not in LB medium - (reply: 10)
558. *** Human Insulin promoter sequence*** - (reply: 2)
559. digested plasmid - (reply: 2)
560. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
561. Subcloning - RE digest and ligation and transformation - (reply: 5)
562. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
563. Making DNA Linkers for cloning purposes - (reply: 1)
564. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
565. Dam-/+ e. coli strains - (reply: 6)
566. cloning short genomic DNA fragments - (reply: 1)
567. finding the protein and nucleotide matches - (reply: 1)
568. DH10B with big construct question - (reply: 2)
569. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
570. cloning to pET28a - (reply: 1)