Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
541. Co-transfection with 2 plasmids - (reply: 10)
542. Low passage HEK293?? - (reply: 3)
543. Vector Digestion Problem - (reply: 4)
544. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
545. Transformation with a big plasmid - (reply: 4)
546. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
547. Three way ligation problem - (reply: 6)
548. problems with recovery of dna from gel and ligation - (reply: 5)
549. Colony PCR and digestion with KpnI - (reply: 10)
550. Greenish tinge to bacterial culture - (reply: 3)
551. SURE E. coli competent cells - (reply: 1)
552. Ligation - (reply: 20)
553. E. coli growing on plates and bot not in LB medium - (reply: 10)
554. *** Human Insulin promoter sequence*** - (reply: 2)
555. digested plasmid - (reply: 2)
556. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
557. Subcloning - RE digest and ligation and transformation - (reply: 5)
558. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
559. Making DNA Linkers for cloning purposes - (reply: 1)
560. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
561. Dam-/+ e. coli strains - (reply: 6)
562. cloning short genomic DNA fragments - (reply: 1)
563. finding the protein and nucleotide matches - (reply: 1)
564. DH10B with big construct question - (reply: 2)
565. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
566. cloning to pET28a - (reply: 1)
567. Weird Library Transformation Results - (reply: 10)
568. Injecting mice with lentivirus - (reply: 1)
569. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
570. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)