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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Vector Digestion Problem - (reply: 4)
542. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
543. Transformation with a big plasmid - (reply: 4)
544. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
545. Three way ligation problem - (reply: 6)
546. problems with recovery of dna from gel and ligation - (reply: 5)
547. Colony PCR and digestion with KpnI - (reply: 10)
548. Greenish tinge to bacterial culture - (reply: 3)
549. SURE E. coli competent cells - (reply: 1)
550. Ligation - (reply: 20)
551. E. coli growing on plates and bot not in LB medium - (reply: 10)
552. *** Human Insulin promoter sequence*** - (reply: 2)
553. digested plasmid - (reply: 2)
554. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
555. Subcloning - RE digest and ligation and transformation - (reply: 5)
556. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
557. Making DNA Linkers for cloning purposes - (reply: 1)
558. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
559. Dam-/+ e. coli strains - (reply: 6)
560. cloning short genomic DNA fragments - (reply: 1)
561. finding the protein and nucleotide matches - (reply: 1)
562. DH10B with big construct question - (reply: 2)
563. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
564. cloning to pET28a - (reply: 1)
565. Weird Library Transformation Results - (reply: 10)
566. Injecting mice with lentivirus - (reply: 1)
567. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
568. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
569. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
570. Ligation into a BAC? - (reply: 2)