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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
542. pINDUCER series - (reply: 1)
543. LZRS vector details - (reply: 1)
544. 12.4kb vector 6.7kb insert - (reply: 2)
545. Stable transfection - (reply: 2)
546. Using normal competent cells in electroporation method? - (reply: 1)
547. an effective way to do a yeast colony pcr - (reply: 2)
548. pGEM-T Easy vector contamination - (reply: 2)
549. recover old transformed DH5 - (reply: 1)
550. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
551. oligo(dt) 15 vs random primers - (reply: 3)
552. I need bynary vector pART27 - (reply: 1)
553. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
554. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
555. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
556. cloning into Pgem t easy - (reply: 1)
557. How to get pure protein of a gene - (reply: 3)
558. How to insert genes into chromosome of E. coli - (reply: 1)
559. Gene synthesis - (reply: 2)
560. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
561. Pls help me with this plasmid!!! - (reply: 1)
562. Problems with Restriction Digest Results - (reply: 7)
563. Methods of plasmid transformation? only heat shock? - (reply: 1)
564. Digestion - (reply: 2)
565. Digestion of Genomic DNA - (reply: 1)
566. Isolation of a Gene of Interest for Subcloning - (reply: 8)
567. E.coli grow on neg. control - (reply: 1)
568. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
569. Need help troubleshooting digest. - (reply: 1)
570. Competent cells question: JM101 vs DH5alpha - (reply: 2)