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Top : New Forum Archives (2009-): : Molecular-Cloning
541. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
542. Pls help me with this plasmid!!! - (reply: 1)
543. Problems with Restriction Digest Results - (reply: 7)
544. Methods of plasmid transformation? only heat shock? - (reply: 1)
545. Digestion - (reply: 2)
546. Digestion of Genomic DNA - (reply: 1)
547. Isolation of a Gene of Interest for Subcloning - (reply: 8)
548. E.coli grow on neg. control - (reply: 1)
549. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
550. Need help troubleshooting digest. - (reply: 1)
551. Competent cells question: JM101 vs DH5alpha - (reply: 2)
552. How to avoid plasmid instability - (reply: 4)
553. Question Help - (reply: 1)
554. Shear-induced damage of plasmid by vortexing - (reply: 1)
555. Problems with blunt end ligation - (reply: 3)
556. Problem with Transforming E. coli DH10Bac - (reply: 8)
557. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
558. Heat inactivate ligase - (reply: 5)
559. DMSO stock of overnight bacterial cultures - (reply: 1)
560. Recommended competent cell for transformation of large plasmids? - (reply: 4)
561. Prolem in screening of hygromycin concentration - (reply: 1)
562. Primers for Introduction of new restriction sites to a vector - (reply: 3)
563. problem in cloning involving partial digestion - (reply: 1)
564. Why perform restriction enzyme digestion again? - (reply: 1)
565. Cotransient Transfections DNA Requirements - (reply: 3)
566. which software to draw the scheme of cloning strategy - (reply: 5)
567. Help with restriction analysis - (reply: 1)
568. Sequencing of PCR product - (reply: 4)
569. KanR sequence - (reply: 2)
570. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)