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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
542. protein is not expessing in HEK 293 cells - (reply: 2)
543. Restriction problems - (reply: 4)
544. cloning a gene in to a plasmid - (reply: 5)
545. no product on gel after restriction digestion - (reply: 3)
546. Gateway technology. - (reply: 4)
547. Primer concentration for cDNA synthesis using GSP - (reply: 1)
548. cloning from cDNA but got a much shorter PCR product - (reply: 4)
549. GeneArt vs Quikchange - (reply: 1)
550. why do some colonies keep growing in the chiller? - (reply: 7)
551. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
552. Difference between types of bacterial culture broths? - (reply: 4)
553. Co-transfection with 2 plasmids - (reply: 10)
554. Low passage HEK293?? - (reply: 3)
555. Vector Digestion Problem - (reply: 4)
556. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
557. Transformation with a big plasmid - (reply: 4)
558. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
559. Three way ligation problem - (reply: 6)
560. problems with recovery of dna from gel and ligation - (reply: 5)
561. Colony PCR and digestion with KpnI - (reply: 10)
562. Greenish tinge to bacterial culture - (reply: 3)
563. SURE E. coli competent cells - (reply: 1)
564. Ligation - (reply: 20)
565. E. coli growing on plates and bot not in LB medium - (reply: 10)
566. *** Human Insulin promoter sequence*** - (reply: 2)
567. digested plasmid - (reply: 2)
568. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
569. Subcloning - RE digest and ligation and transformation - (reply: 5)
570. Plasmid digested with RE used to transform...what will happen? - (reply: 14)