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Top : New Forum Archives (2009-): : Molecular-Cloning
541. cloning a gene in to a plasmid - (reply: 5)
542. no product on gel after restriction digestion - (reply: 3)
543. Gateway technology. - (reply: 4)
544. Primer concentration for cDNA synthesis using GSP - (reply: 1)
545. cloning from cDNA but got a much shorter PCR product - (reply: 4)
546. GeneArt vs Quikchange - (reply: 1)
547. why do some colonies keep growing in the chiller? - (reply: 7)
548. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
549. Difference between types of bacterial culture broths? - (reply: 4)
550. Co-transfection with 2 plasmids - (reply: 10)
551. Low passage HEK293?? - (reply: 3)
552. Vector Digestion Problem - (reply: 4)
553. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
554. Transformation with a big plasmid - (reply: 4)
555. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
556. Three way ligation problem - (reply: 6)
557. problems with recovery of dna from gel and ligation - (reply: 5)
558. Colony PCR and digestion with KpnI - (reply: 10)
559. Greenish tinge to bacterial culture - (reply: 3)
560. SURE E. coli competent cells - (reply: 1)
561. Ligation - (reply: 20)
562. E. coli growing on plates and bot not in LB medium - (reply: 10)
563. *** Human Insulin promoter sequence*** - (reply: 2)
564. digested plasmid - (reply: 2)
565. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
566. Subcloning - RE digest and ligation and transformation - (reply: 5)
567. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
568. Making DNA Linkers for cloning purposes - (reply: 1)
569. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
570. Dam-/+ e. coli strains - (reply: 6)