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Top : New Forum Archives (2009-): : Molecular-Cloning
541. SURE E. coli competent cells - (reply: 1)
542. Ligation - (reply: 20)
543. E. coli growing on plates and bot not in LB medium - (reply: 10)
544. *** Human Insulin promoter sequence*** - (reply: 2)
545. digested plasmid - (reply: 2)
546. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
547. Subcloning - RE digest and ligation and transformation - (reply: 5)
548. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
549. Making DNA Linkers for cloning purposes - (reply: 1)
550. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
551. Dam-/+ e. coli strains - (reply: 6)
552. cloning short genomic DNA fragments - (reply: 1)
553. finding the protein and nucleotide matches - (reply: 1)
554. DH10B with big construct question - (reply: 2)
555. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
556. cloning to pET28a - (reply: 1)
557. Weird Library Transformation Results - (reply: 10)
558. Injecting mice with lentivirus - (reply: 1)
559. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
560. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
561. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
562. Ligation into a BAC? - (reply: 2)
563. oligo continually appears less on gel compared to other same load - (reply: 2)
564. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
565. plasmid tetR and tet promoter - (reply: 2)
566. unable to amplify an infectious retrovirus clone - (reply: 4)
567. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
568. No bands at all after double digestion - (reply: 1)
569. Digest for insert orientation - (reply: 4)
570. Colonies from transformation plate are not growing...:( - (reply: 5)