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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Help designing primers for use in In-fushion cloning - (reply: 4)
542. Repeat of desired mutation in cloning product - (reply: 1)
543. vector dephosphorylation - (reply: 5)
544. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
545. DH10BAC - (reply: 3)
546. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
547. protein is not expessing in HEK 293 cells - (reply: 2)
548. Restriction problems - (reply: 4)
549. cloning a gene in to a plasmid - (reply: 5)
550. no product on gel after restriction digestion - (reply: 3)
551. Gateway technology. - (reply: 4)
552. Primer concentration for cDNA synthesis using GSP - (reply: 1)
553. cloning from cDNA but got a much shorter PCR product - (reply: 4)
554. GeneArt vs Quikchange - (reply: 1)
555. why do some colonies keep growing in the chiller? - (reply: 7)
556. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
557. Difference between types of bacterial culture broths? - (reply: 4)
558. Co-transfection with 2 plasmids - (reply: 10)
559. Low passage HEK293?? - (reply: 3)
560. Vector Digestion Problem - (reply: 4)
561. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
562. Transformation with a big plasmid - (reply: 4)
563. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
564. Three way ligation problem - (reply: 6)
565. problems with recovery of dna from gel and ligation - (reply: 5)
566. Colony PCR and digestion with KpnI - (reply: 10)
567. Greenish tinge to bacterial culture - (reply: 3)
568. SURE E. coli competent cells - (reply: 1)
569. Ligation - (reply: 20)
570. E. coli growing on plates and bot not in LB medium - (reply: 10)