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Top : New Forum Archives (2009-): : Molecular-Cloning
541. no product on gel after restriction digestion - (reply: 3)
542. Gateway technology. - (reply: 4)
543. Primer concentration for cDNA synthesis using GSP - (reply: 1)
544. cloning from cDNA but got a much shorter PCR product - (reply: 4)
545. GeneArt vs Quikchange - (reply: 1)
546. why do some colonies keep growing in the chiller? - (reply: 7)
547. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
548. Difference between types of bacterial culture broths? - (reply: 4)
549. Co-transfection with 2 plasmids - (reply: 10)
550. Low passage HEK293?? - (reply: 3)
551. Vector Digestion Problem - (reply: 4)
552. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
553. Transformation with a big plasmid - (reply: 4)
554. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
555. Three way ligation problem - (reply: 6)
556. problems with recovery of dna from gel and ligation - (reply: 5)
557. Colony PCR and digestion with KpnI - (reply: 10)
558. Greenish tinge to bacterial culture - (reply: 3)
559. SURE E. coli competent cells - (reply: 1)
560. Ligation - (reply: 20)
561. E. coli growing on plates and bot not in LB medium - (reply: 10)
562. *** Human Insulin promoter sequence*** - (reply: 2)
563. digested plasmid - (reply: 2)
564. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
565. Subcloning - RE digest and ligation and transformation - (reply: 5)
566. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
567. Making DNA Linkers for cloning purposes - (reply: 1)
568. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
569. Dam-/+ e. coli strains - (reply: 6)
570. cloning short genomic DNA fragments - (reply: 1)