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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Sequencing of PCR product - (reply: 4)
542. KanR sequence - (reply: 2)
543. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
544. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
545. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
546. Plasmid digestion after transformation - (reply: 4)
547. Blunt end ligation insert:vector ratio - (reply: 1)
548. PCRed on restriction sites -- how do I purify it? - (reply: 8)
549. Puzzle about plamid and its digest, plz help - (reply: 8)
550. Smear above plasmid dna - (reply: 3)
551. restriction digestion buffers contaminated with dnase - (reply: 3)
552. Bacterial Induction - (reply: 1)
553. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
554. smear restriction digest - (reply: 1)
555. How to set the Sonicator to 20%amplitude? - (reply: 3)
556. No DNA after midiprep - (reply: 2)
557. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
558. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
559. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
560. Impact of primer sequence on TA cloning - (reply: 3)
561. Maximum length of cDNA from RT reaction - (reply: 6)
562. overlapping PCR protocol - (reply: 12)
563. cloning an AT rich gene - (reply: 3)
564. Cloning two genes in the same plasmid - (reply: 4)
565. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
566. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
567. Problem: Cloning DNA shuffled band - (reply: 2)
568. shRNA to primary keratinocytes - (reply: 1)
569. Enzymetic digestion with ApaI and HincII - (reply: 2)
570. Ligation gel picture interpretation - (reply: 4)