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541. No colonies after transformation! - (reply: 2)
542. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
543. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
544. Help Miniprep kit SIGMA - (reply: 2)
545. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
546. A question about pBR322 and pUC ori - (reply: 3)
547. Digestion of mini-prep by mistake - (reply: 3)
548. Cloning issue - (reply: 3)
549. Cohesive end filling after CIAP treatment - (reply: 2)
550. Looking for Pfu Taq for high GC-content template - (reply: 7)
551. questions about transformation - (reply: 4)
552. genotype lysogen DE3 - (reply: 1)
553. Need help! - Problem with right insertion! (reply: 1)
554. cloning a large insert - (reply: 5)
555. Transposase contamination in cloning - (reply: 7)
556. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
557. How long can I store cleaved/digested vector? - (reply: 9)
558. Cloning of a gene containing 31 exon - (reply: 1)
559. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
560. Optimum Molar ratio for 3-way ligation - (reply: 5)
561. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
562. cloning help - (reply: 3)
563. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
564. A gene is not inserted into the Plasmid - (reply: 2)
565. Air bubbles during electroporation - (reply: 1)
566. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
567. Colonies without inserts - (reply: 9)
568. Transformants Don't Grow - (reply: 4)
569. control DNA transformation- using pGEM kit - transformation (reply: 4)
570. Topo Cloning - Understanding the principle (reply: 6)
542. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
543. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
544. Help Miniprep kit SIGMA - (reply: 2)
545. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
546. A question about pBR322 and pUC ori - (reply: 3)
547. Digestion of mini-prep by mistake - (reply: 3)
548. Cloning issue - (reply: 3)
549. Cohesive end filling after CIAP treatment - (reply: 2)
550. Looking for Pfu Taq for high GC-content template - (reply: 7)
551. questions about transformation - (reply: 4)
552. genotype lysogen DE3 - (reply: 1)
553. Need help! - Problem with right insertion! (reply: 1)
554. cloning a large insert - (reply: 5)
555. Transposase contamination in cloning - (reply: 7)
556. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
557. How long can I store cleaved/digested vector? - (reply: 9)
558. Cloning of a gene containing 31 exon - (reply: 1)
559. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
560. Optimum Molar ratio for 3-way ligation - (reply: 5)
561. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
562. cloning help - (reply: 3)
563. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
564. A gene is not inserted into the Plasmid - (reply: 2)
565. Air bubbles during electroporation - (reply: 1)
566. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
567. Colonies without inserts - (reply: 9)
568. Transformants Don't Grow - (reply: 4)
569. control DNA transformation- using pGEM kit - transformation (reply: 4)
570. Topo Cloning - Understanding the principle (reply: 6)