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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Primer concentration for cDNA synthesis using GSP - (reply: 1)
542. cloning from cDNA but got a much shorter PCR product - (reply: 4)
543. GeneArt vs Quikchange - (reply: 1)
544. why do some colonies keep growing in the chiller? - (reply: 7)
545. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
546. Difference between types of bacterial culture broths? - (reply: 4)
547. Co-transfection with 2 plasmids - (reply: 10)
548. Low passage HEK293?? - (reply: 3)
549. Vector Digestion Problem - (reply: 4)
550. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
551. Transformation with a big plasmid - (reply: 4)
552. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
553. Three way ligation problem - (reply: 6)
554. problems with recovery of dna from gel and ligation - (reply: 5)
555. Colony PCR and digestion with KpnI - (reply: 10)
556. Greenish tinge to bacterial culture - (reply: 3)
557. SURE E. coli competent cells - (reply: 1)
558. Ligation - (reply: 20)
559. E. coli growing on plates and bot not in LB medium - (reply: 10)
560. *** Human Insulin promoter sequence*** - (reply: 2)
561. digested plasmid - (reply: 2)
562. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
563. Subcloning - RE digest and ligation and transformation - (reply: 5)
564. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
565. Making DNA Linkers for cloning purposes - (reply: 1)
566. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
567. Dam-/+ e. coli strains - (reply: 6)
568. cloning short genomic DNA fragments - (reply: 1)
569. finding the protein and nucleotide matches - (reply: 1)
570. DH10B with big construct question - (reply: 2)