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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Restriction problems - (reply: 4)
542. cloning a gene in to a plasmid - (reply: 5)
543. no product on gel after restriction digestion - (reply: 3)
544. Gateway technology. - (reply: 4)
545. Primer concentration for cDNA synthesis using GSP - (reply: 1)
546. cloning from cDNA but got a much shorter PCR product - (reply: 4)
547. GeneArt vs Quikchange - (reply: 1)
548. why do some colonies keep growing in the chiller? - (reply: 7)
549. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
550. Difference between types of bacterial culture broths? - (reply: 4)
551. Co-transfection with 2 plasmids - (reply: 10)
552. Low passage HEK293?? - (reply: 3)
553. Vector Digestion Problem - (reply: 4)
554. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
555. Transformation with a big plasmid - (reply: 4)
556. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
557. Three way ligation problem - (reply: 6)
558. problems with recovery of dna from gel and ligation - (reply: 5)
559. Colony PCR and digestion with KpnI - (reply: 10)
560. Greenish tinge to bacterial culture - (reply: 3)
561. SURE E. coli competent cells - (reply: 1)
562. Ligation - (reply: 20)
563. E. coli growing on plates and bot not in LB medium - (reply: 10)
564. *** Human Insulin promoter sequence*** - (reply: 2)
565. digested plasmid - (reply: 2)
566. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
567. Subcloning - RE digest and ligation and transformation - (reply: 5)
568. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
569. Making DNA Linkers for cloning purposes - (reply: 1)
570. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)