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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Linearising plasmid before transfection? - (reply: 2)
542. Smear in plasmid sample - (reply: 9)
543. about a book - (reply: 1)
544. Cloning Problem! I need help - (reply: 3)
545. Help designing primers for use in In-fushion cloning - (reply: 4)
546. Repeat of desired mutation in cloning product - (reply: 1)
547. vector dephosphorylation - (reply: 5)
548. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
549. DH10BAC - (reply: 3)
550. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
551. protein is not expessing in HEK 293 cells - (reply: 2)
552. Restriction problems - (reply: 4)
553. cloning a gene in to a plasmid - (reply: 5)
554. no product on gel after restriction digestion - (reply: 3)
555. Gateway technology. - (reply: 4)
556. Primer concentration for cDNA synthesis using GSP - (reply: 1)
557. cloning from cDNA but got a much shorter PCR product - (reply: 4)
558. GeneArt vs Quikchange - (reply: 1)
559. why do some colonies keep growing in the chiller? - (reply: 7)
560. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
561. Difference between types of bacterial culture broths? - (reply: 4)
562. Co-transfection with 2 plasmids - (reply: 10)
563. Low passage HEK293?? - (reply: 3)
564. Vector Digestion Problem - (reply: 4)
565. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
566. Transformation with a big plasmid - (reply: 4)
567. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
568. Three way ligation problem - (reply: 6)
569. problems with recovery of dna from gel and ligation - (reply: 5)
570. Colony PCR and digestion with KpnI - (reply: 10)