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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
542. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
543. Ligation into a BAC? - (reply: 2)
544. oligo continually appears less on gel compared to other same load - (reply: 2)
545. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
546. plasmid tetR and tet promoter - (reply: 2)
547. unable to amplify an infectious retrovirus clone - (reply: 4)
548. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
549. No bands at all after double digestion - (reply: 1)
550. Digest for insert orientation - (reply: 4)
551. Colonies from transformation plate are not growing...:( - (reply: 5)
552. Problem with ligating a small insert (LoxP site) - (reply: 5)
553. Is the genomic DNA similar among different cell lines? - (reply: 4)
554. molecular cloning - (reply: 3)
555. Swich selection in plasmid - (reply: 3)
556. Sub-cloning and transformation - (reply: 17)
557. cloning without phosphorylation - (reply: 7)
558. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
559. A good vector to make gene library? - (reply: 5)
560. problems with midiprep - (reply: 3)
561. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
562. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
563. 900bp insertion into PIRESnEO,problem - (reply: 2)
564. fusion of cDNA expressing protein with gfp - (reply: 1)
565. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
566. Problem with plasmids transformation - (reply: 6)
567. Cloning genetic sequences of proteins. - (reply: 1)
568. problem of restriction enzyme digestion - (reply: 3)
569. Site-directed, PCR works but no colonies - (reply: 2)
570. Simoltanious Multi-restriction digestion - (reply: 3)