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Top : New Forum Archives (2009-): : Molecular-Cloning
541. Cloning Problem! I need help - (reply: 3)
542. Help designing primers for use in In-fushion cloning - (reply: 4)
543. Repeat of desired mutation in cloning product - (reply: 1)
544. vector dephosphorylation - (reply: 5)
545. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
546. DH10BAC - (reply: 3)
547. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
548. protein is not expessing in HEK 293 cells - (reply: 2)
549. Restriction problems - (reply: 4)
550. cloning a gene in to a plasmid - (reply: 5)
551. no product on gel after restriction digestion - (reply: 3)
552. Gateway technology. - (reply: 4)
553. Primer concentration for cDNA synthesis using GSP - (reply: 1)
554. cloning from cDNA but got a much shorter PCR product - (reply: 4)
555. GeneArt vs Quikchange - (reply: 1)
556. why do some colonies keep growing in the chiller? - (reply: 7)
557. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
558. Difference between types of bacterial culture broths? - (reply: 4)
559. Co-transfection with 2 plasmids - (reply: 10)
560. Low passage HEK293?? - (reply: 3)
561. Vector Digestion Problem - (reply: 4)
562. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
563. Transformation with a big plasmid - (reply: 4)
564. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
565. Three way ligation problem - (reply: 6)
566. problems with recovery of dna from gel and ligation - (reply: 5)
567. Colony PCR and digestion with KpnI - (reply: 10)
568. Greenish tinge to bacterial culture - (reply: 3)
569. SURE E. coli competent cells - (reply: 1)
570. Ligation - (reply: 20)