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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
2. pET32b+ vector issue - (reply: 1)
3. Subcloning issue - (reply: 2)
4. Need to add 12 bp at the beginning of the sequence - (reply: 4)
5. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 2)
6. Problem with possible leaky expression in cloning - (reply: 4)
7. Luciferase assay cloning - (reply: 1)
8. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
9. How stable is double digested plasmid - (reply: 6)
10. Problems with ligation - (reply: 1)
11. Problem with restriction digestion of cloned PCR product - (reply: 5)
12. SLIC Cloning Failure - (reply: 2)
13. Failed Gibson transformation - (reply: 7)
14. how to check for intron-exon junctions - (reply: 2)
15. Lower Plasmid Yields - (reply: 4)
16. Using SDS for DNA electrophoresis - (reply: 2)
17. Bacterial knockout by double crossover - (reply: 4)
18. Polymerase mixture for blunt ended fragments - (reply: 2)
19. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
20. Question in gateway system, LR reaction - (reply: 5)
21. Problem with subcloning genescript synthesized genes - (reply: 1)
22. Flourescence visualization - (reply: 2)
23. Gel Purification: When is it safe to pause? - (reply: 2)
24. pUC and pSC101 vectors in the same cell - (reply: 3)
25. DNA amount for RE reaction - (reply: 3)
26. Whatman FTA cards - (reply: 1)
27. Trouble with TOPO-Cloning - (reply: 3)
28. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
29. Comparing DNA fragments using restriction map - (reply: 1)
30. Doing a double digest - (reply: 3)