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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Problem with possible leaky expression in cloning - (reply: 4)
2. Luciferase assay cloning - (reply: 1)
3. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
4. How stable is double digested plasmid - (reply: 6)
5. Problems with ligation - (reply: 1)
6. Problem with restriction digestion of cloned PCR product - (reply: 5)
7. SLIC Cloning Failure - (reply: 2)
8. Failed Gibson transformation - (reply: 7)
9. how to check for intron-exon junctions - (reply: 2)
10. Lower Plasmid Yields - (reply: 4)
11. Using SDS for DNA electrophoresis - (reply: 2)
12. Bacterial knockout by double crossover - (reply: 1)
13. Polymerase mixture for blunt ended fragments - (reply: 2)
14. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
15. Question in gateway system, LR reaction - (reply: 5)
16. Problem with subcloning genescript synthesized genes - (reply: 1)
17. Flourescence visualization - (reply: 2)
18. Gel Purification: When is it safe to pause? - (reply: 2)
19. pUC and pSC101 vectors in the same cell - (reply: 3)
20. DNA amount for RE reaction - (reply: 3)
21. Whatman FTA cards - (reply: 1)
22. Trouble with TOPO-Cloning - (reply: 3)
23. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
24. Comparing DNA fragments using restriction map - (reply: 1)
25. Doing a double digest - (reply: 3)
26. Types of igation controls - (reply: 6)
27. Problem with double digest cloning - (reply: 3)
28. Weird results with bacterial transformation - (reply: 8)
29. Site Directed Mutagenesis - no product - (reply: 4)
30. Problems sequencing ligation product - (reply: 5)