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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Plasmid purification for Gibson Assembly - (reply: 2)
2. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
3. TA cloning..Urgent Help!! - (reply: 4)
4. Can someone please explain this to me? - (reply: 1)
5. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
6. TetA as a counter-selection marker - (reply: 1)
7. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
8. pET32b+ vector issue - (reply: 1)
9. Subcloning issue - (reply: 2)
10. Splicing DNA fragments - (reply: 2)
11. Need to add 12 bp at the beginning of the sequence - (reply: 4)
12. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
13. Problem with possible leaky expression in cloning - (reply: 4)
14. Luciferase assay cloning - (reply: 2)
15. Selecting bacteria with Puromycin - (reply: 1)
16. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
17. How stable is double digested plasmid - (reply: 7)
18. Problems with ligation - (reply: 2)
19. Problem with restriction digestion of cloned PCR product - (reply: 5)
20. SLIC Cloning Failure - (reply: 2)
21. Failed Gibson transformation - (reply: 7)
22. how to check for intron-exon junctions - (reply: 2)
23. Lower Plasmid Yields - (reply: 5)
24. Cleaning loading dye for PCR sequencing - (reply: 1)
25. Using SDS for DNA electrophoresis - (reply: 3)
26. Bacterial knockout by double crossover - (reply: 4)
27. Polymerase mixture for blunt ended fragments - (reply: 2)
28. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
29. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
30. Question in gateway system, LR reaction - (reply: 5)