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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Double digestion problem with PUCsp vector - (reply: 1)
2. How much plasmid for transformation? - (reply: 3)
3. RE digestion and ligation troubleshooting. - (reply: 1)
4. replicating a mammalian expression vector in bacteria - (reply: 3)
5. Cloning of an unknown gene - (reply: 3)
6. cloning problems using in-fusion kit - (reply: 2)
7. Mutation in one chain of homodimer - (reply: 3)
8. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
9. Alternative competent cells for QuikChange kit - (reply: 2)
10. Ligation Mistake - (reply: 4)
11. Cloning - Ligation problem - (reply: 3)
12. How to clone a 2A peptide between two ORFs? - (reply: 2)
13. preparing a vector for transfection - (reply: 2)
14. Ligation of two PCR products - (reply: 1)
15. TA Cloning - (reply: 11)
16. Troubleshootting lentiviral transduction. - (reply: 3)
17. Trouble with PCR using ligation mix as template? - (reply: 3)
18. Suitable ratio of vector and insert in cloning - (reply: 5)
19. The right lentivirus plasmid for overexpression in human cells - (reply: 3)
20. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
21. plasmid repositories - (reply: 2)
22. How to insert PreScission protease into a vector - (reply: 1)
23. Why the insert become shorter after inserting into plasmid? - (reply: 2)
24. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
25. Thermo scientific T4 DNA ligase product help - (reply: 3)
26. Cloning a big insert in a small vector - (reply: 2)
27. Transformation E.Coli help - (reply: 2)
28. Exonucleases that won't harm circular dsDNA - (reply: 1)
29. How to produce transgenic mice? - (reply: 10)
30. No colonies on plate after liagtion - (reply: 7)