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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Can mutations be created during cloning? - (reply: 2)
2. confusing with where to put primers and which ordination - (reply: 4)
3. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
4. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
5. RBS - (reply: 1)
6. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
7. Gel for my ligation - (reply: 1)
8. Amplifying from pcr products - (reply: 7)
9. Design the primers - (reply: 10)
10. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
11. How two fragments joint together when doing fusion PCR? - (reply: 4)
12. Working backwards from a construct? - (reply: 1)
13. disappearing bases - (reply: 4)
14. Mutagenesis PCR - (reply: 9)
15. Digest two sites located next to each other - (reply: 5)
16. cDNA Library Construction by using kits and Sequencing - (reply: 1)
17. Looking for software that permutes codons to find new restriction sites - (reply: 4)
18. Transforming dna from agarose gel - (reply: 3)
19. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
20. ccdb toxin - (reply: 15)
21. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
22. annealing oligos - (reply: 3)
23. Does a restriction enzyme cut at a related site? - (reply: 5)
24. primers for cloning - (reply: 1)
25. Vector-insert ratio - (reply: 6)
26. Cloning contamination - (reply: 3)
27. plasmid construction - (reply: 2)
28. primer design with restriction enzyme - (reply: 1)
29. vector propagation in E coli - (reply: 1)
30. Restriction to remove background - (reply: 4)