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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Gel Purification: When is it safe to pause? - (reply: 1)
2. pUC and pSC101 vectors in the same cell - (reply: 3)
3. DNA amount for RE reaction - (reply: 3)
4. Whatman FTA cards - (reply: 1)
5. Trouble with TOPO-Cloning - (reply: 3)
6. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
7. Comparing DNA fragments using restriction map - (reply: 1)
8. Doing a double digest - (reply: 3)
9. Types of igation controls - (reply: 6)
10. Problem with double digest cloning - (reply: 3)
11. Weird results with bacterial transformation - (reply: 6)
12. Site Directed Mutagenesis - no product - (reply: 4)
13. Problems sequencing ligation product - (reply: 5)
14. Faster method to screeen for positive colonies. - (reply: 8)
15. 260/230 ratio, does it matter? - (reply: 4)
16. Double digestion problem with PUCsp vector - (reply: 1)
17. How much plasmid for transformation? - (reply: 8)
18. RE digestion and ligation troubleshooting. - (reply: 1)
19. replicating a mammalian expression vector in bacteria - (reply: 3)
20. Cloning of an unknown gene - (reply: 3)
21. cloning problems using in-fusion kit - (reply: 5)
22. Mutation in one chain of homodimer - (reply: 4)
23. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
24. Alternative competent cells for QuikChange kit - (reply: 2)
25. Ligation Mistake - (reply: 4)
26. Cloning - Ligation problem - (reply: 3)
27. How to clone a 2A peptide between two ORFs? - (reply: 2)
28. preparing a vector for transfection - (reply: 2)
29. Ligation of two PCR products - (reply: 1)
30. TA Cloning - (reply: 11)