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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Insert in frame - (reply: 1)
2. PCRed-vector approach to tag switching - (reply: 1)
3. High copy vs low copy plasmids: which one causes less toxicity? - (reply: 2)
4. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
5. low copy number plasmid purification kit - (reply: 4)
6. Neomycin/Kanamycin Resistant gene - (reply: 2)
7. Plasmid analysis in limited conditions - (reply: 6)
8. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
9. Troubleshooting with restriction enzymes during cloning - (reply: 5)
10. Transformation - (reply: 3)
11. Plasmid distributors for Luciferase Assay? - (reply: 2)
12. Ligate a very small insert into a vector - (reply: 3)
13. Restriction enzyme - (reply: 1)
14. Plasmid dna size - (reply: 3)
15. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
16. Express two different membrane proteins on the same promoter - (reply: 1)
17. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
18. [Overexpression] Without upstream transcription terminator - (reply: 4)
19. How large need homology arms to be? - (reply: 1)
20. Ampicillin LB agar reheating and reusing - (reply: 2)
21. Using pB2GW7,0 - (reply: 2)
22. propagating commercial chemically competent cells - (reply: 2)
23. Plasmid purification for Gibson Assembly - (reply: 2)
24. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
25. TA cloning..Urgent Help!! - (reply: 4)
26. Can someone please explain this to me? - (reply: 2)
27. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
28. TetA as a counter-selection marker - (reply: 1)
29. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
30. pET32b+ vector issue - (reply: 1)