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1. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
2. Cloning large fragments - (reply: 1)
3. Transformation colonies does not contain insert - (reply: 3)
4. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
5. Need urgent advise.. - (reply: 3)
6. Cloning Mitochondrial Targeting Sequence - (reply: 4)
7. problem in cloning - (reply: 2)
8. TA cloning: ligation problem or toxic construct? - (reply: 4)
9. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
10. Do I need to sequence after digestion? - (reply: 9)
11. quick change mutagenesis...... - (reply: 15)
12. Problem with double digestion: one enzyme is not working - (reply: 8)
13. Transient Transfection Controls (Gateway Technology) - (reply: 4)
14. Problems with crossover PCR - (reply: 2)
15. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
16. Two genes, same MCS - (reply: 1)
17. Including ATG in cloning? - (reply: 1)
18. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 1)
19. Low copy vector for library generation - (reply: 2)
20. Can't find a recombinant after ligating - (reply: 4)
21. problems with plasmid miniprep - (reply: 8)
22. gene cloning - (reply: 1)
23. Is ligation really necessary? - (reply: 6)
24. problem with ligation of linker/adaptor - (reply: 2)
25. GFP -weak signal -why? - (reply: 1)
26. Ligation troubleshooting! Please help! - (reply: 5)
27. Truncated gene Cloning - (reply: 1)
28. Screening for ligation and transformation result - (reply: 2)
29. Problem with 3 way ligation - (reply: 2)
30. Query regarding primers for quick change mutagenesis - (reply: 3)
2. Cloning large fragments - (reply: 1)
3. Transformation colonies does not contain insert - (reply: 3)
4. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
5. Need urgent advise.. - (reply: 3)
6. Cloning Mitochondrial Targeting Sequence - (reply: 4)
7. problem in cloning - (reply: 2)
8. TA cloning: ligation problem or toxic construct? - (reply: 4)
9. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
10. Do I need to sequence after digestion? - (reply: 9)
11. quick change mutagenesis...... - (reply: 15)
12. Problem with double digestion: one enzyme is not working - (reply: 8)
13. Transient Transfection Controls (Gateway Technology) - (reply: 4)
14. Problems with crossover PCR - (reply: 2)
15. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
16. Two genes, same MCS - (reply: 1)
17. Including ATG in cloning? - (reply: 1)
18. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 1)
19. Low copy vector for library generation - (reply: 2)
20. Can't find a recombinant after ligating - (reply: 4)
21. problems with plasmid miniprep - (reply: 8)
22. gene cloning - (reply: 1)
23. Is ligation really necessary? - (reply: 6)
24. problem with ligation of linker/adaptor - (reply: 2)
25. GFP -weak signal -why? - (reply: 1)
26. Ligation troubleshooting! Please help! - (reply: 5)
27. Truncated gene Cloning - (reply: 1)
28. Screening for ligation and transformation result - (reply: 2)
29. Problem with 3 way ligation - (reply: 2)
30. Query regarding primers for quick change mutagenesis - (reply: 3)