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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
2. TA cloning..Urgent Help!! - (reply: 3)
3. Can someone please explain this to me? - (reply: 1)
4. Problem with polyA retrotranscription: I have truncated products - (reply: 1)
5. TetA as a counter-selection marker - (reply: 1)
6. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
7. pET32b+ vector issue - (reply: 1)
8. Subcloning issue - (reply: 2)
9. Splicing DNA fragments - (reply: 1)
10. Need to add 12 bp at the beginning of the sequence - (reply: 4)
11. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
12. Problem with possible leaky expression in cloning - (reply: 4)
13. Luciferase assay cloning - (reply: 2)
14. Selecting bacteria with Puromycin - (reply: 1)
15. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
16. How stable is double digested plasmid - (reply: 7)
17. Problems with ligation - (reply: 2)
18. Problem with restriction digestion of cloned PCR product - (reply: 5)
19. SLIC Cloning Failure - (reply: 2)
20. Failed Gibson transformation - (reply: 7)
21. how to check for intron-exon junctions - (reply: 2)
22. Lower Plasmid Yields - (reply: 5)
23. Cleaning loading dye for PCR sequencing - (reply: 1)
24. Using SDS for DNA electrophoresis - (reply: 3)
25. Bacterial knockout by double crossover - (reply: 4)
26. Polymerase mixture for blunt ended fragments - (reply: 2)
27. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
28. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
29. Question in gateway system, LR reaction - (reply: 5)
30. Problem with subcloning genescript synthesized genes - (reply: 2)