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Top : New Forum Archives (2009-): : Molecular-Cloning
1. transformation - (reply: 2)
2. problem in sequencing of gene - (reply: 3)
3. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
4. Plasmid Methylation - (reply: 3)
5. Problem with BamHI Digestion - (reply: 7)
6. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
7. How to find promoter sequence ... - (reply: 3)
8. Sticky and blunt end ligation in single reaction? - (reply: 2)
9. Restriction double digest - (reply: 1)
10. Choice of AMP 100 versus AMP 50 - (reply: 1)
11. Why are there two origin of replications in plasmids - (reply: 3)
12. How can we expand molecular cloning further? - (reply: 3)
13. Subtle change in insert size after cloning - (reply: 6)
14. lactic acid bacteria - cloning - (reply: 2)
15. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
16. All I get is supercoiled DNA... - (reply: 5)
17. Help Quesion - (reply: 1)
18. No colonies after self Circularization ligation - (reply: 2)
19. Clean ligation controls but no insert - (reply: 12)
20. ratios insert/vector - (reply: 3)
21. Can't isolate plasmid from cloned E.coli - (reply: 3)
22. Genomic DNA quantification using the NanoDrop - (reply: 3)
23. Competent cells with Hanahan's method - (reply: 2)
24. cloning issues - (reply: 3)
25. Is important the direction of IRES-GFP in cloning? - (reply: 1)
26. What are the error-rates of the TA cloning Kits? - (reply: 8)
27. Can mutations be created during cloning? - (reply: 7)
28. confusing with where to put primers and which ordination - (reply: 4)
29. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
30. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)