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Top : New Forum Archives (2009-): : Molecular-Cloning
1. Question in gateway system, LR reaction - (reply: 5)
2. Problem with subcloning genescript synthesized genes - (reply: 1)
3. Flourescence visualization - (reply: 2)
4. Gel Purification: When is it safe to pause? - (reply: 2)
5. pUC and pSC101 vectors in the same cell - (reply: 3)
6. DNA amount for RE reaction - (reply: 3)
7. Whatman FTA cards - (reply: 1)
8. Trouble with TOPO-Cloning - (reply: 3)
9. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
10. Comparing DNA fragments using restriction map - (reply: 1)
11. Doing a double digest - (reply: 3)
12. Types of igation controls - (reply: 6)
13. Problem with double digest cloning - (reply: 3)
14. Weird results with bacterial transformation - (reply: 8)
15. Site Directed Mutagenesis - no product - (reply: 4)
16. Problems sequencing ligation product - (reply: 5)
17. Faster method to screeen for positive colonies. - (reply: 8)
18. 260/230 ratio, does it matter? - (reply: 4)
19. Double digestion problem with PUCsp vector - (reply: 1)
20. How much plasmid for transformation? - (reply: 8)
21. RE digestion and ligation troubleshooting. - (reply: 1)
22. replicating a mammalian expression vector in bacteria - (reply: 3)
23. Cloning of an unknown gene - (reply: 3)
24. cloning problems using in-fusion kit - (reply: 5)
25. Mutation in one chain of homodimer - (reply: 4)
26. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
27. Alternative competent cells for QuikChange kit - (reply: 2)
28. Ligation Mistake - (reply: 4)
29. Cloning - Ligation problem - (reply: 3)
30. How to clone a 2A peptide between two ORFs? - (reply: 2)