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Top : New Forum Archives (2009-): : Molecular-Cloning
1. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 1)
2. Why the insert become shorter after inserting into plasmid? - (reply: 1)
3. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
4. Thermo scientific T4 DNA ligase product help - (reply: 3)
5. Cloning a big insert in a small vector - (reply: 2)
6. Transformation E.Coli help - (reply: 2)
7. Exonucleases that won't harm circular dsDNA - (reply: 1)
8. How to produce transgenic mice? - (reply: 10)
9. No colonies on plate after liagtion - (reply: 7)
10. Question: Can I cut out rb glob PA Terminator - (reply: 1)
11. Low Yield Miniprep - (reply: 5)
12. question about Clonetech Retro-X Tet-on system - (reply: 3)
13. quiaquick gel extraction troubleshooting - (reply: 1)
14. Insert direction in TA cloning - (reply: 2)
15. Transformation in Staphylococcus epidermidis - (reply: 1)
16. Plasmids recombining - (reply: 3)
17. troubleshooting for a simple SDM - (reply: 2)
18. Should I proceed with clone selection? - (reply: 2)
19. Need help in codon optimization... - (reply: 1)
20. transformation - (reply: 2)
21. problem in sequencing of gene - (reply: 3)
22. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
23. Plasmid Methylation - (reply: 3)
24. Problem with BamHI Digestion - (reply: 7)
25. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
26. How to find promoter sequence ... - (reply: 3)
27. Sticky and blunt end ligation in single reaction? - (reply: 2)
28. Restriction double digest - (reply: 1)
29. Choice of AMP 100 versus AMP 50 - (reply: 1)
30. Why are there two origin of replications in plasmids - (reply: 3)