Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1. Site Directed Mutagenesis - no product - (reply: 4)
2. Problems sequencing ligation product - (reply: 5)
3. Faster method to screeen for positive colonies. - (reply: 7)
4. 260/230 ratio, does it matter? - (reply: 4)
5. Double digestion problem with PUCsp vector - (reply: 1)
6. How much plasmid for transformation? - (reply: 8)
7. RE digestion and ligation troubleshooting. - (reply: 1)
8. replicating a mammalian expression vector in bacteria - (reply: 3)
9. Cloning of an unknown gene - (reply: 3)
10. cloning problems using in-fusion kit - (reply: 5)
11. Mutation in one chain of homodimer - (reply: 3)
12. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
13. Alternative competent cells for QuikChange kit - (reply: 2)
14. Ligation Mistake - (reply: 4)
15. Cloning - Ligation problem - (reply: 3)
16. How to clone a 2A peptide between two ORFs? - (reply: 2)
17. preparing a vector for transfection - (reply: 2)
18. Ligation of two PCR products - (reply: 1)
19. TA Cloning - (reply: 11)
20. Troubleshootting lentiviral transduction. - (reply: 3)
21. Trouble with PCR using ligation mix as template? - (reply: 3)
22. Suitable ratio of vector and insert in cloning - (reply: 5)
23. The right lentivirus plasmid for overexpression in human cells - (reply: 3)
24. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
25. plasmid repositories - (reply: 2)
26. How to insert PreScission protease into a vector - (reply: 1)
27. Why the insert become shorter after inserting into plasmid? - (reply: 2)
28. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
29. Thermo scientific T4 DNA ligase product help - (reply: 3)
30. Cloning a big insert in a small vector - (reply: 2)