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Top : New Forum Archives (2009-): : Molecular-Cloning
31. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
32. Restriction and ligation time interval - (reply: 1)
33. Problem shrna cloning, bad sequencing results - (reply: 1)
34. Lentivirus copy number - (reply: 7)
35. Genomic -Lactamase? Blue White screening not working - (reply: 1)
36. direction of the expression units in multi-gene vectors - (reply: 1)
37. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
38. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
39. Measuring pcr fragments in a gel - (reply: 1)
40. gel electrophoresis - (reply: 4)
41. three questions about CRISPR knowout - (reply: 2)
42. Help needed for Ligation/Transformation - (reply: 1)
43. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
44. ClaI restriction enzyme efficency - (reply: 1)
45. Blunt end cloning - (reply: 12)
46. sequantial digest - (reply: 25)
47. Temperature sensitivity of Plasmids - (reply: 1)
48. DMSO in restriction digest - (reply: 1)
49. Molecular cloning - (reply: 5)
50. Nuclease free water for cloning - (reply: 8)
51. Directional cloning 2bps overhang - (reply: 3)
52. I need help designing homology arms for CRISPR - (reply: 3)
53. Partial digest XhoI NotI NEB - (reply: 6)
54. removing part from plasmid - (reply: 18)
55. Start codon after promoter - (reply: 1)
56. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
57. Purpose of PolyA in cloning - (reply: 1)
58. Ligation failure? I ran some on a gel - (reply: 6)
59. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
60. Cloning into pVIVO2 vector - (reply: 8)