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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Why the insert become shorter after inserting into plasmid? - (reply: 2)
32. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
33. Thermo scientific T4 DNA ligase product help - (reply: 3)
34. Cloning a big insert in a small vector - (reply: 2)
35. Transformation E.Coli help - (reply: 2)
36. Exonucleases that won't harm circular dsDNA - (reply: 1)
37. How to produce transgenic mice? - (reply: 10)
38. No colonies on plate after liagtion - (reply: 7)
39. Question: Can I cut out rb glob PA Terminator - (reply: 1)
40. Low Yield Miniprep - (reply: 5)
41. question about Clonetech Retro-X Tet-on system - (reply: 3)
42. Clonetech Retro-X Tet-on system - (reply: 1)
43. quiaquick gel extraction troubleshooting - (reply: 1)
44. Insert direction in TA cloning - (reply: 2)
45. Transformation in Staphylococcus epidermidis - (reply: 1)
46. Plasmids recombining - (reply: 3)
47. troubleshooting for a simple SDM - (reply: 2)
48. Should I proceed with clone selection? - (reply: 2)
49. Need help in codon optimization... - (reply: 1)
50. transformation - (reply: 2)
51. problem in sequencing of gene - (reply: 3)
52. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
53. Plasmid Methylation - (reply: 3)
54. Problem with BamHI Digestion - (reply: 7)
55. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
56. How to find promoter sequence ... - (reply: 3)
57. Sticky and blunt end ligation in single reaction? - (reply: 2)
58. Restriction double digest - (reply: 1)
59. Choice of AMP 100 versus AMP 50 - (reply: 1)
60. Why are there two origin of replications in plasmids - (reply: 3)