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Top : New Forum Archives (2009-): : Molecular-Cloning
31. RBS - (reply: 1)
32. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
33. Gel for my ligation - (reply: 1)
34. Amplifying from pcr products - (reply: 7)
35. Design the primers - (reply: 10)
36. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
37. How two fragments joint together when doing fusion PCR? - (reply: 4)
38. Working backwards from a construct? - (reply: 1)
39. disappearing bases - (reply: 4)
40. Mutagenesis PCR - (reply: 9)
41. Digest two sites located next to each other - (reply: 5)
42. cDNA Library Construction by using kits and Sequencing - (reply: 1)
43. Looking for software that permutes codons to find new restriction sites - (reply: 4)
44. Transforming dna from agarose gel - (reply: 3)
45. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
46. ccdb toxin - (reply: 15)
47. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
48. annealing oligos - (reply: 3)
49. Does a restriction enzyme cut at a related site? - (reply: 5)
50. primers for cloning - (reply: 1)
51. Vector-insert ratio - (reply: 6)
52. Cloning contamination - (reply: 3)
53. plasmid construction - (reply: 2)
54. primer design with restriction enzyme - (reply: 1)
55. vector propagation in E coli - (reply: 1)
56. Restriction to remove background - (reply: 4)
57. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
58. Restriction and ligation time interval - (reply: 1)
59. Problem shrna cloning, bad sequencing results - (reply: 1)
60. Lentivirus copy number - (reply: 7)