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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Subcloning issue - (reply: 2)
32. Splicing DNA fragments - (reply: 2)
33. Need to add 12 bp at the beginning of the sequence - (reply: 4)
34. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
35. Problem with possible leaky expression in cloning - (reply: 4)
36. Luciferase assay cloning - (reply: 2)
37. Selecting bacteria with Puromycin - (reply: 1)
38. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
39. How stable is double digested plasmid - (reply: 7)
40. Problems with ligation - (reply: 2)
41. Problem with restriction digestion of cloned PCR product - (reply: 5)
42. SLIC Cloning Failure - (reply: 2)
43. Failed Gibson transformation - (reply: 7)
44. how to check for intron-exon junctions - (reply: 2)
45. Lower Plasmid Yields - (reply: 5)
46. Cleaning loading dye for PCR sequencing - (reply: 1)
47. Using SDS for DNA electrophoresis - (reply: 3)
48. Bacterial knockout by double crossover - (reply: 4)
49. Polymerase mixture for blunt ended fragments - (reply: 2)
50. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
51. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
52. Question in gateway system, LR reaction - (reply: 5)
53. Problem with subcloning genescript synthesized genes - (reply: 2)
54. Flourescence visualization - (reply: 2)
55. Gel Purification: When is it safe to pause? - (reply: 2)
56. pUC and pSC101 vectors in the same cell - (reply: 3)
57. DNA amount for RE reaction - (reply: 3)
58. Whatman FTA cards - (reply: 1)
59. Trouble with TOPO-Cloning - (reply: 3)
60. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)