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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
32. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
33. Measuring pcr fragments in a gel - (reply: 1)
34. gel electrophoresis - (reply: 4)
35. three questions about CRISPR knowout - (reply: 2)
36. Help needed for Ligation/Transformation - (reply: 1)
37. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
38. ClaI restriction enzyme efficency - (reply: 1)
39. Blunt end cloning - (reply: 12)
40. sequantial digest - (reply: 25)
41. Temperature sensitivity of Plasmids - (reply: 1)
42. DMSO in restriction digest - (reply: 1)
43. Molecular cloning - (reply: 5)
44. Nuclease free water for cloning - (reply: 8)
45. Directional cloning 2bps overhang - (reply: 3)
46. I need help designing homology arms for CRISPR - (reply: 3)
47. Partial digest XhoI NotI NEB - (reply: 6)
48. removing part from plasmid - (reply: 18)
49. Start codon after promoter - (reply: 1)
50. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
51. Purpose of PolyA in cloning - (reply: 1)
52. Ligation failure? I ran some on a gel - (reply: 6)
53. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
54. Cloning into pVIVO2 vector - (reply: 8)
55. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
56. Cloning worked, but the expression vector is weird - (reply: 6)
57. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
58. site directed mutagenesis - (reply: 3)
59. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
60. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)