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Top : New Forum Archives (2009-): : Molecular-Cloning
31. TetA as a counter-selection marker - (reply: 1)
32. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
33. pET32b+ vector issue - (reply: 1)
34. Subcloning issue - (reply: 2)
35. Splicing DNA fragments - (reply: 2)
36. Need to add 12 bp at the beginning of the sequence - (reply: 4)
37. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
38. Problem with possible leaky expression in cloning - (reply: 4)
39. Luciferase assay cloning - (reply: 2)
40. Selecting bacteria with Puromycin - (reply: 1)
41. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
42. How stable is double digested plasmid - (reply: 7)
43. Problems with ligation - (reply: 2)
44. Problem with restriction digestion of cloned PCR product - (reply: 5)
45. SLIC Cloning Failure - (reply: 2)
46. Failed Gibson transformation - (reply: 7)
47. how to check for intron-exon junctions - (reply: 2)
48. Lower Plasmid Yields - (reply: 5)
49. Cleaning loading dye for PCR sequencing - (reply: 1)
50. Using SDS for DNA electrophoresis - (reply: 3)
51. Bacterial knockout by double crossover - (reply: 4)
52. Polymerase mixture for blunt ended fragments - (reply: 2)
53. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
54. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
55. Question in gateway system, LR reaction - (reply: 5)
56. Problem with subcloning genescript synthesized genes - (reply: 2)
57. Flourescence visualization - (reply: 2)
58. Gel Purification: When is it safe to pause? - (reply: 2)
59. pUC and pSC101 vectors in the same cell - (reply: 3)
60. DNA amount for RE reaction - (reply: 3)