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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Types of igation controls - (reply: 6)
32. Problem with double digest cloning - (reply: 3)
33. Weird results with bacterial transformation - (reply: 8)
34. Site Directed Mutagenesis - no product - (reply: 4)
35. Problems sequencing ligation product - (reply: 5)
36. Faster method to screeen for positive colonies. - (reply: 8)
37. 260/230 ratio, does it matter? - (reply: 4)
38. Double digestion problem with PUCsp vector - (reply: 1)
39. How much plasmid for transformation? - (reply: 8)
40. RE digestion and ligation troubleshooting. - (reply: 1)
41. replicating a mammalian expression vector in bacteria - (reply: 3)
42. Cloning of an unknown gene - (reply: 3)
43. cloning problems using in-fusion kit - (reply: 5)
44. Mutation in one chain of homodimer - (reply: 4)
45. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
46. Alternative competent cells for QuikChange kit - (reply: 2)
47. Ligation Mistake - (reply: 4)
48. Cloning - Ligation problem - (reply: 3)
49. How to clone a 2A peptide between two ORFs? - (reply: 2)
50. preparing a vector for transfection - (reply: 2)
51. Ligation of two PCR products - (reply: 1)
52. TA Cloning - (reply: 11)
53. Troubleshootting lentiviral transduction. - (reply: 3)
54. Trouble with PCR using ligation mix as template? - (reply: 3)
55. Suitable ratio of vector and insert in cloning - (reply: 5)
56. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
57. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
58. plasmid repositories - (reply: 2)
59. How to insert PreScission protease into a vector - (reply: 1)
60. Why the insert become shorter after inserting into plasmid? - (reply: 2)