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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Polymerase mixture for blunt ended fragments - (reply: 2)
32. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
33. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
34. Question in gateway system, LR reaction - (reply: 5)
35. Problem with subcloning genescript synthesized genes - (reply: 2)
36. Flourescence visualization - (reply: 2)
37. Gel Purification: When is it safe to pause? - (reply: 2)
38. pUC and pSC101 vectors in the same cell - (reply: 3)
39. DNA amount for RE reaction - (reply: 3)
40. Whatman FTA cards - (reply: 1)
41. Trouble with TOPO-Cloning - (reply: 3)
42. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
43. Comparing DNA fragments using restriction map - (reply: 1)
44. Doing a double digest - (reply: 3)
45. Types of igation controls - (reply: 6)
46. Problem with double digest cloning - (reply: 3)
47. Weird results with bacterial transformation - (reply: 9)
48. Site Directed Mutagenesis - no product - (reply: 4)
49. Problems sequencing ligation product - (reply: 5)
50. Faster method to screeen for positive colonies. - (reply: 8)
51. 260/230 ratio, does it matter? - (reply: 4)
52. Double digestion problem with PUCsp vector - (reply: 1)
53. How much plasmid for transformation? - (reply: 8)
54. RE digestion and ligation troubleshooting. - (reply: 1)
55. replicating a mammalian expression vector in bacteria - (reply: 3)
56. Cloning of an unknown gene - (reply: 3)
57. cloning problems using in-fusion kit - (reply: 5)
58. Mutation in one chain of homodimer - (reply: 4)
59. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
60. Alternative competent cells for QuikChange kit - (reply: 2)