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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Problem with subcloning genescript synthesized genes - (reply: 2)
32. Flourescence visualization - (reply: 2)
33. Gel Purification: When is it safe to pause? - (reply: 2)
34. pUC and pSC101 vectors in the same cell - (reply: 3)
35. DNA amount for RE reaction - (reply: 3)
36. Whatman FTA cards - (reply: 1)
37. Trouble with TOPO-Cloning - (reply: 3)
38. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
39. Comparing DNA fragments using restriction map - (reply: 1)
40. Doing a double digest - (reply: 3)
41. Types of igation controls - (reply: 6)
42. Problem with double digest cloning - (reply: 3)
43. Weird results with bacterial transformation - (reply: 9)
44. Site Directed Mutagenesis - no product - (reply: 4)
45. Problems sequencing ligation product - (reply: 5)
46. Faster method to screeen for positive colonies. - (reply: 8)
47. 260/230 ratio, does it matter? - (reply: 4)
48. Double digestion problem with PUCsp vector - (reply: 1)
49. How much plasmid for transformation? - (reply: 8)
50. RE digestion and ligation troubleshooting. - (reply: 1)
51. replicating a mammalian expression vector in bacteria - (reply: 3)
52. Cloning of an unknown gene - (reply: 3)
53. cloning problems using in-fusion kit - (reply: 5)
54. Mutation in one chain of homodimer - (reply: 4)
55. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
56. Alternative competent cells for QuikChange kit - (reply: 2)
57. Ligation Mistake - (reply: 4)
58. Cloning - Ligation problem - (reply: 3)
59. How to clone a 2A peptide between two ORFs? - (reply: 2)
60. preparing a vector for transfection - (reply: 2)