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Top : New Forum Archives (2009-): : Molecular-Cloning
31. how to check for intron-exon junctions - (reply: 2)
32. Lower Plasmid Yields - (reply: 5)
33. Cleaning loading dye for PCR sequencing - (reply: 1)
34. Using SDS for DNA electrophoresis - (reply: 3)
35. Bacterial knockout by double crossover - (reply: 4)
36. Polymerase mixture for blunt ended fragments - (reply: 2)
37. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
38. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
39. Question in gateway system, LR reaction - (reply: 5)
40. Problem with subcloning genescript synthesized genes - (reply: 2)
41. Flourescence visualization - (reply: 2)
42. Gel Purification: When is it safe to pause? - (reply: 2)
43. pUC and pSC101 vectors in the same cell - (reply: 3)
44. DNA amount for RE reaction - (reply: 3)
45. Whatman FTA cards - (reply: 1)
46. Trouble with TOPO-Cloning - (reply: 3)
47. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
48. Comparing DNA fragments using restriction map - (reply: 1)
49. Doing a double digest - (reply: 3)
50. Types of igation controls - (reply: 6)
51. Problem with double digest cloning - (reply: 3)
52. Weird results with bacterial transformation - (reply: 9)
53. Site Directed Mutagenesis - no product - (reply: 4)
54. Problems sequencing ligation product - (reply: 5)
55. Faster method to screeen for positive colonies. - (reply: 8)
56. 260/230 ratio, does it matter? - (reply: 4)
57. Double digestion problem with PUCsp vector - (reply: 1)
58. How much plasmid for transformation? - (reply: 8)
59. RE digestion and ligation troubleshooting. - (reply: 1)
60. replicating a mammalian expression vector in bacteria - (reply: 3)