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31. What is NEB's CutSmart Buffer? - (reply: 5)
32. Enzyme cutting: low efficiency - (reply: 5)
33. Issue with Xba1 and Dam methylation - (reply: 7)
34. Restriction site rearrangement - (reply: 1)
35. Mutation in cloning - (reply: 6)
36. Must the terminator be in frame? - (reply: 1)
37. sequencing problems - (reply: 2)
38. Understand the basics of molecular cloning - (reply: 6)
39. Maintainence of transformed cells - (reply: 5)
40. Restriction digest band shift? - (reply: 1)
41. problem in cloning PCR primer design with restriction site - (reply: 4)
42. quick change mutagenesis...... - (reply: 1)
43. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
44. Help for pGEM-T easy vector ligation problem - (reply: 2)
45. Co-transformation - (reply: 2)
46. Problems regarding point mutations.... - (reply: 4)
47. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
48. pkp 59 vector - (reply: 2)
49. Vector insert ratio in cloning - (reply: 3)
50. polymerase to use for cloning - (reply: 4)
51. Oligo insert cloning - (reply: 3)
52. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
53. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
54. How to select competent E. coli cells? - (reply: 7)
55. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
56. cDNA amplification problem - (reply: 4)
57. Ligation: two basic questions - (reply: 1)
58. Unexpected base after deletion mutagenesis - (reply: 3)
59. Confirmation of ligation - (reply: 7)
60. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
32. Enzyme cutting: low efficiency - (reply: 5)
33. Issue with Xba1 and Dam methylation - (reply: 7)
34. Restriction site rearrangement - (reply: 1)
35. Mutation in cloning - (reply: 6)
36. Must the terminator be in frame? - (reply: 1)
37. sequencing problems - (reply: 2)
38. Understand the basics of molecular cloning - (reply: 6)
39. Maintainence of transformed cells - (reply: 5)
40. Restriction digest band shift? - (reply: 1)
41. problem in cloning PCR primer design with restriction site - (reply: 4)
42. quick change mutagenesis...... - (reply: 1)
43. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
44. Help for pGEM-T easy vector ligation problem - (reply: 2)
45. Co-transformation - (reply: 2)
46. Problems regarding point mutations.... - (reply: 4)
47. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
48. pkp 59 vector - (reply: 2)
49. Vector insert ratio in cloning - (reply: 3)
50. polymerase to use for cloning - (reply: 4)
51. Oligo insert cloning - (reply: 3)
52. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
53. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
54. How to select competent E. coli cells? - (reply: 7)
55. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
56. cDNA amplification problem - (reply: 4)
57. Ligation: two basic questions - (reply: 1)
58. Unexpected base after deletion mutagenesis - (reply: 3)
59. Confirmation of ligation - (reply: 7)
60. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)