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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Can't isolate plasmid from cloned E.coli - (reply: 3)
32. Genomic DNA quantification using the NanoDrop - (reply: 3)
33. Competent cells with Hanahan's method - (reply: 2)
34. cloning issues - (reply: 3)
35. Is important the direction of IRES-GFP in cloning? - (reply: 1)
36. What are the error-rates of the TA cloning Kits? - (reply: 8)
37. Can mutations be created during cloning? - (reply: 7)
38. confusing with where to put primers and which ordination - (reply: 4)
39. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
40. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
41. RBS - (reply: 1)
42. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
43. Gel for my ligation - (reply: 1)
44. Amplifying from pcr products - (reply: 7)
45. Design the primers - (reply: 10)
46. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
47. How two fragments joint together when doing fusion PCR? - (reply: 4)
48. Working backwards from a construct? - (reply: 1)
49. disappearing bases - (reply: 4)
50. Mutagenesis PCR - (reply: 9)
51. Digest two sites located next to each other - (reply: 5)
52. cDNA Library Construction by using kits and Sequencing - (reply: 1)
53. Looking for software that permutes codons to find new restriction sites - (reply: 4)
54. Transforming dna from agarose gel - (reply: 3)
55. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
56. ccdb toxin - (reply: 15)
57. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
58. annealing oligos - (reply: 3)
59. Does a restriction enzyme cut at a related site? - (reply: 5)
60. primers for cloning - (reply: 1)