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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Problem with restriction digestion of cloned PCR product - (reply: 5)
32. SLIC Cloning Failure - (reply: 2)
33. Failed Gibson transformation - (reply: 7)
34. how to check for intron-exon junctions - (reply: 2)
35. Lower Plasmid Yields - (reply: 5)
36. Cleaning loading dye for PCR sequencing - (reply: 1)
37. Using SDS for DNA electrophoresis - (reply: 3)
38. Bacterial knockout by double crossover - (reply: 4)
39. Polymerase mixture for blunt ended fragments - (reply: 2)
40. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
41. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
42. Question in gateway system, LR reaction - (reply: 5)
43. Problem with subcloning genescript synthesized genes - (reply: 2)
44. Flourescence visualization - (reply: 2)
45. Gel Purification: When is it safe to pause? - (reply: 2)
46. pUC and pSC101 vectors in the same cell - (reply: 3)
47. DNA amount for RE reaction - (reply: 3)
48. Whatman FTA cards - (reply: 1)
49. Trouble with TOPO-Cloning - (reply: 3)
50. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
51. Comparing DNA fragments using restriction map - (reply: 1)
52. Doing a double digest - (reply: 3)
53. Types of igation controls - (reply: 6)
54. Problem with double digest cloning - (reply: 3)
55. Weird results with bacterial transformation - (reply: 9)
56. Site Directed Mutagenesis - no product - (reply: 4)
57. Problems sequencing ligation product - (reply: 5)
58. Faster method to screeen for positive colonies. - (reply: 8)
59. 260/230 ratio, does it matter? - (reply: 4)
60. Double digestion problem with PUCsp vector - (reply: 1)