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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Looking for software that permutes codons to find new restriction sites - (reply: 4)
32. Transforming dna from agarose gel - (reply: 3)
33. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
34. ccdb toxin - (reply: 15)
35. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
36. annealing oligos - (reply: 3)
37. Does a restriction enzyme cut at a related site? - (reply: 5)
38. primers for cloning - (reply: 1)
39. Vector-insert ratio - (reply: 6)
40. Cloning contamination - (reply: 3)
41. plasmid construction - (reply: 2)
42. primer design with restriction enzyme - (reply: 1)
43. vector propagation in E coli - (reply: 1)
44. Restriction to remove background - (reply: 4)
45. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
46. Restriction and ligation time interval - (reply: 1)
47. Problem shrna cloning, bad sequencing results - (reply: 1)
48. Lentivirus copy number - (reply: 7)
49. Genomic -Lactamase? Blue White screening not working - (reply: 1)
50. direction of the expression units in multi-gene vectors - (reply: 1)
51. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
52. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
53. Measuring pcr fragments in a gel - (reply: 1)
54. gel electrophoresis - (reply: 4)
55. three questions about CRISPR knowout - (reply: 2)
56. Help needed for Ligation/Transformation - (reply: 1)
57. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
58. ClaI restriction enzyme efficency - (reply: 1)
59. Blunt end cloning - (reply: 12)
60. sequantial digest - (reply: 25)