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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Lower Plasmid Yields - (reply: 5)
32. Cleaning loading dye for PCR sequencing - (reply: 1)
33. Using SDS for DNA electrophoresis - (reply: 3)
34. Bacterial knockout by double crossover - (reply: 4)
35. Polymerase mixture for blunt ended fragments - (reply: 2)
36. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
37. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
38. Question in gateway system, LR reaction - (reply: 5)
39. Problem with subcloning genescript synthesized genes - (reply: 2)
40. Flourescence visualization - (reply: 2)
41. Gel Purification: When is it safe to pause? - (reply: 2)
42. pUC and pSC101 vectors in the same cell - (reply: 3)
43. DNA amount for RE reaction - (reply: 3)
44. Whatman FTA cards - (reply: 1)
45. Trouble with TOPO-Cloning - (reply: 3)
46. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
47. Comparing DNA fragments using restriction map - (reply: 1)
48. Doing a double digest - (reply: 3)
49. Types of igation controls - (reply: 6)
50. Problem with double digest cloning - (reply: 3)
51. Weird results with bacterial transformation - (reply: 9)
52. Site Directed Mutagenesis - no product - (reply: 4)
53. Problems sequencing ligation product - (reply: 5)
54. Faster method to screeen for positive colonies. - (reply: 8)
55. 260/230 ratio, does it matter? - (reply: 4)
56. Double digestion problem with PUCsp vector - (reply: 1)
57. How much plasmid for transformation? - (reply: 8)
58. RE digestion and ligation troubleshooting. - (reply: 1)
59. replicating a mammalian expression vector in bacteria - (reply: 3)
60. Cloning of an unknown gene - (reply: 3)