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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Cleaning loading dye for PCR sequencing - (reply: 1)
32. Using SDS for DNA electrophoresis - (reply: 3)
33. Bacterial knockout by double crossover - (reply: 4)
34. Polymerase mixture for blunt ended fragments - (reply: 2)
35. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
36. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
37. Question in gateway system, LR reaction - (reply: 5)
38. Problem with subcloning genescript synthesized genes - (reply: 2)
39. Flourescence visualization - (reply: 2)
40. Gel Purification: When is it safe to pause? - (reply: 2)
41. pUC and pSC101 vectors in the same cell - (reply: 3)
42. DNA amount for RE reaction - (reply: 3)
43. Whatman FTA cards - (reply: 1)
44. Trouble with TOPO-Cloning - (reply: 3)
45. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
46. Comparing DNA fragments using restriction map - (reply: 1)
47. Doing a double digest - (reply: 3)
48. Types of igation controls - (reply: 6)
49. Problem with double digest cloning - (reply: 3)
50. Weird results with bacterial transformation - (reply: 9)
51. Site Directed Mutagenesis - no product - (reply: 4)
52. Problems sequencing ligation product - (reply: 5)
53. Faster method to screeen for positive colonies. - (reply: 8)
54. 260/230 ratio, does it matter? - (reply: 4)
55. Double digestion problem with PUCsp vector - (reply: 1)
56. How much plasmid for transformation? - (reply: 8)
57. RE digestion and ligation troubleshooting. - (reply: 1)
58. replicating a mammalian expression vector in bacteria - (reply: 3)
59. Cloning of an unknown gene - (reply: 3)
60. cloning problems using in-fusion kit - (reply: 5)