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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Using SDS for DNA electrophoresis - (reply: 3)
32. Bacterial knockout by double crossover - (reply: 4)
33. Polymerase mixture for blunt ended fragments - (reply: 2)
34. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
35. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
36. Question in gateway system, LR reaction - (reply: 5)
37. Problem with subcloning genescript synthesized genes - (reply: 2)
38. Flourescence visualization - (reply: 2)
39. Gel Purification: When is it safe to pause? - (reply: 2)
40. pUC and pSC101 vectors in the same cell - (reply: 3)
41. DNA amount for RE reaction - (reply: 3)
42. Whatman FTA cards - (reply: 1)
43. Trouble with TOPO-Cloning - (reply: 3)
44. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
45. Comparing DNA fragments using restriction map - (reply: 1)
46. Doing a double digest - (reply: 3)
47. Types of igation controls - (reply: 6)
48. Problem with double digest cloning - (reply: 3)
49. Weird results with bacterial transformation - (reply: 9)
50. Site Directed Mutagenesis - no product - (reply: 4)
51. Problems sequencing ligation product - (reply: 5)
52. Faster method to screeen for positive colonies. - (reply: 8)
53. 260/230 ratio, does it matter? - (reply: 4)
54. Double digestion problem with PUCsp vector - (reply: 1)
55. How much plasmid for transformation? - (reply: 8)
56. RE digestion and ligation troubleshooting. - (reply: 1)
57. replicating a mammalian expression vector in bacteria - (reply: 3)
58. Cloning of an unknown gene - (reply: 3)
59. cloning problems using in-fusion kit - (reply: 5)
60. Mutation in one chain of homodimer - (reply: 4)