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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Flourescence visualization - (reply: 2)
32. Gel Purification: When is it safe to pause? - (reply: 2)
33. pUC and pSC101 vectors in the same cell - (reply: 3)
34. DNA amount for RE reaction - (reply: 3)
35. Whatman FTA cards - (reply: 1)
36. Trouble with TOPO-Cloning - (reply: 3)
37. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
38. Comparing DNA fragments using restriction map - (reply: 1)
39. Doing a double digest - (reply: 3)
40. Types of igation controls - (reply: 6)
41. Problem with double digest cloning - (reply: 3)
42. Weird results with bacterial transformation - (reply: 9)
43. Site Directed Mutagenesis - no product - (reply: 4)
44. Problems sequencing ligation product - (reply: 5)
45. Faster method to screeen for positive colonies. - (reply: 8)
46. 260/230 ratio, does it matter? - (reply: 4)
47. Double digestion problem with PUCsp vector - (reply: 1)
48. How much plasmid for transformation? - (reply: 8)
49. RE digestion and ligation troubleshooting. - (reply: 1)
50. replicating a mammalian expression vector in bacteria - (reply: 3)
51. Cloning of an unknown gene - (reply: 3)
52. cloning problems using in-fusion kit - (reply: 5)
53. Mutation in one chain of homodimer - (reply: 4)
54. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
55. Alternative competent cells for QuikChange kit - (reply: 2)
56. Ligation Mistake - (reply: 4)
57. Cloning - Ligation problem - (reply: 3)
58. How to clone a 2A peptide between two ORFs? - (reply: 2)
59. preparing a vector for transfection - (reply: 2)
60. Ligation of two PCR products - (reply: 1)