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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Failed Gibson transformation - (reply: 7)
32. how to check for intron-exon junctions - (reply: 2)
33. Lower Plasmid Yields - (reply: 5)
34. Cleaning loading dye for PCR sequencing - (reply: 1)
35. Using SDS for DNA electrophoresis - (reply: 3)
36. Bacterial knockout by double crossover - (reply: 4)
37. Polymerase mixture for blunt ended fragments - (reply: 2)
38. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
39. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
40. Question in gateway system, LR reaction - (reply: 5)
41. Problem with subcloning genescript synthesized genes - (reply: 2)
42. Flourescence visualization - (reply: 2)
43. Gel Purification: When is it safe to pause? - (reply: 2)
44. pUC and pSC101 vectors in the same cell - (reply: 3)
45. DNA amount for RE reaction - (reply: 3)
46. Whatman FTA cards - (reply: 1)
47. Trouble with TOPO-Cloning - (reply: 3)
48. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
49. Comparing DNA fragments using restriction map - (reply: 1)
50. Doing a double digest - (reply: 3)
51. Types of igation controls - (reply: 6)
52. Problem with double digest cloning - (reply: 3)
53. Weird results with bacterial transformation - (reply: 9)
54. Site Directed Mutagenesis - no product - (reply: 4)
55. Problems sequencing ligation product - (reply: 5)
56. Faster method to screeen for positive colonies. - (reply: 8)
57. 260/230 ratio, does it matter? - (reply: 4)
58. Double digestion problem with PUCsp vector - (reply: 1)
59. How much plasmid for transformation? - (reply: 8)
60. RE digestion and ligation troubleshooting. - (reply: 1)