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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Splicing DNA fragments - (reply: 2)
32. Need to add 12 bp at the beginning of the sequence - (reply: 4)
33. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
34. Problem with possible leaky expression in cloning - (reply: 4)
35. Luciferase assay cloning - (reply: 2)
36. Selecting bacteria with Puromycin - (reply: 1)
37. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
38. How stable is double digested plasmid - (reply: 7)
39. Problems with ligation - (reply: 2)
40. Problem with restriction digestion of cloned PCR product - (reply: 5)
41. SLIC Cloning Failure - (reply: 2)
42. Failed Gibson transformation - (reply: 7)
43. how to check for intron-exon junctions - (reply: 2)
44. Lower Plasmid Yields - (reply: 5)
45. Cleaning loading dye for PCR sequencing - (reply: 1)
46. Using SDS for DNA electrophoresis - (reply: 3)
47. Bacterial knockout by double crossover - (reply: 4)
48. Polymerase mixture for blunt ended fragments - (reply: 2)
49. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
50. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
51. Question in gateway system, LR reaction - (reply: 5)
52. Problem with subcloning genescript synthesized genes - (reply: 2)
53. Flourescence visualization - (reply: 2)
54. Gel Purification: When is it safe to pause? - (reply: 2)
55. pUC and pSC101 vectors in the same cell - (reply: 3)
56. DNA amount for RE reaction - (reply: 3)
57. Whatman FTA cards - (reply: 1)
58. Trouble with TOPO-Cloning - (reply: 3)
59. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
60. Comparing DNA fragments using restriction map - (reply: 1)