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Top : New Forum Archives (2009-): : Molecular-Cloning
31. How can we expand molecular cloning further? - (reply: 3)
32. Subtle change in insert size after cloning - (reply: 6)
33. lactic acid bacteria - cloning - (reply: 2)
34. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
35. All I get is supercoiled DNA... - (reply: 5)
36. Help Quesion - (reply: 1)
37. No colonies after self Circularization ligation - (reply: 2)
38. Clean ligation controls but no insert - (reply: 14)
39. ratios insert/vector - (reply: 3)
40. Can't isolate plasmid from cloned E.coli - (reply: 3)
41. Genomic DNA quantification using the NanoDrop - (reply: 3)
42. Competent cells with Hanahan's method - (reply: 2)
43. cloning issues - (reply: 3)
44. Is important the direction of IRES-GFP in cloning? - (reply: 1)
45. What are the error-rates of the TA cloning Kits? - (reply: 8)
46. Can mutations be created during cloning? - (reply: 7)
47. confusing with where to put primers and which ordination - (reply: 4)
48. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
49. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
50. RBS - (reply: 1)
51. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
52. Gel for my ligation - (reply: 1)
53. Amplifying from pcr products - (reply: 7)
54. Design the primers - (reply: 10)
55. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
56. How two fragments joint together when doing fusion PCR? - (reply: 4)
57. Working backwards from a construct? - (reply: 1)
58. disappearing bases - (reply: 4)
59. Mutagenesis PCR - (reply: 9)
60. Digest two sites located next to each other - (reply: 5)