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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Question in gateway system, LR reaction - (reply: 5)
32. Problem with subcloning genescript synthesized genes - (reply: 2)
33. Flourescence visualization - (reply: 2)
34. Gel Purification: When is it safe to pause? - (reply: 2)
35. pUC and pSC101 vectors in the same cell - (reply: 3)
36. DNA amount for RE reaction - (reply: 3)
37. Whatman FTA cards - (reply: 1)
38. Trouble with TOPO-Cloning - (reply: 3)
39. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
40. Comparing DNA fragments using restriction map - (reply: 1)
41. Doing a double digest - (reply: 3)
42. Types of igation controls - (reply: 6)
43. Problem with double digest cloning - (reply: 3)
44. Weird results with bacterial transformation - (reply: 9)
45. Site Directed Mutagenesis - no product - (reply: 4)
46. Problems sequencing ligation product - (reply: 5)
47. Faster method to screeen for positive colonies. - (reply: 8)
48. 260/230 ratio, does it matter? - (reply: 4)
49. Double digestion problem with PUCsp vector - (reply: 1)
50. How much plasmid for transformation? - (reply: 8)
51. RE digestion and ligation troubleshooting. - (reply: 1)
52. replicating a mammalian expression vector in bacteria - (reply: 3)
53. Cloning of an unknown gene - (reply: 3)
54. cloning problems using in-fusion kit - (reply: 5)
55. Mutation in one chain of homodimer - (reply: 4)
56. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
57. Alternative competent cells for QuikChange kit - (reply: 2)
58. Ligation Mistake - (reply: 4)
59. Cloning - Ligation problem - (reply: 3)
60. How to clone a 2A peptide between two ORFs? - (reply: 2)