Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
31. annealing oligos - (reply: 3)
32. Does a restriction enzyme cut at a related site? - (reply: 5)
33. primers for cloning - (reply: 1)
34. Vector-insert ratio - (reply: 6)
35. Cloning contamination - (reply: 3)
36. plasmid construction - (reply: 2)
37. primer design with restriction enzyme - (reply: 1)
38. vector propagation in E coli - (reply: 1)
39. Restriction to remove background - (reply: 4)
40. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
41. Restriction and ligation time interval - (reply: 1)
42. Problem shrna cloning, bad sequencing results - (reply: 1)
43. Lentivirus copy number - (reply: 7)
44. Genomic -Lactamase? Blue White screening not working - (reply: 1)
45. direction of the expression units in multi-gene vectors - (reply: 1)
46. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
47. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
48. Measuring pcr fragments in a gel - (reply: 1)
49. gel electrophoresis - (reply: 4)
50. three questions about CRISPR knowout - (reply: 2)
51. Help needed for Ligation/Transformation - (reply: 1)
52. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
53. ClaI restriction enzyme efficency - (reply: 1)
54. Blunt end cloning - (reply: 12)
55. sequantial digest - (reply: 25)
56. Temperature sensitivity of Plasmids - (reply: 1)
57. DMSO in restriction digest - (reply: 1)
58. Molecular cloning - (reply: 5)
59. Nuclease free water for cloning - (reply: 8)
60. Directional cloning 2bps overhang - (reply: 3)