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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Selecting bacteria with Puromycin - (reply: 1)
32. After Gibson assembly or T4-Ligation obtained colonies do not grow anymore - (reply: 1)
33. How stable is double digested plasmid - (reply: 7)
34. Problems with ligation - (reply: 2)
35. Problem with restriction digestion of cloned PCR product - (reply: 5)
36. SLIC Cloning Failure - (reply: 2)
37. Failed Gibson transformation - (reply: 7)
38. how to check for intron-exon junctions - (reply: 2)
39. Lower Plasmid Yields - (reply: 5)
40. Cleaning loading dye for PCR sequencing - (reply: 1)
41. Using SDS for DNA electrophoresis - (reply: 3)
42. Bacterial knockout by double crossover - (reply: 4)
43. Polymerase mixture for blunt ended fragments - (reply: 2)
44. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
45. Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
46. Question in gateway system, LR reaction - (reply: 5)
47. Problem with subcloning genescript synthesized genes - (reply: 2)
48. Flourescence visualization - (reply: 2)
49. Gel Purification: When is it safe to pause? - (reply: 2)
50. pUC and pSC101 vectors in the same cell - (reply: 3)
51. DNA amount for RE reaction - (reply: 3)
52. Whatman FTA cards - (reply: 1)
53. Trouble with TOPO-Cloning - (reply: 3)
54. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
55. Comparing DNA fragments using restriction map - (reply: 1)
56. Doing a double digest - (reply: 3)
57. Types of igation controls - (reply: 6)
58. Problem with double digest cloning - (reply: 3)
59. Weird results with bacterial transformation - (reply: 9)
60. Site Directed Mutagenesis - no product - (reply: 4)