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Top : New Forum Archives (2009-): : Molecular-Cloning
31. Comparing DNA fragments using restriction map - (reply: 1)
32. Doing a double digest - (reply: 3)
33. Types of igation controls - (reply: 6)
34. Problem with double digest cloning - (reply: 3)
35. Weird results with bacterial transformation - (reply: 8)
36. Site Directed Mutagenesis - no product - (reply: 4)
37. Problems sequencing ligation product - (reply: 5)
38. Faster method to screeen for positive colonies. - (reply: 8)
39. 260/230 ratio, does it matter? - (reply: 4)
40. Double digestion problem with PUCsp vector - (reply: 1)
41. How much plasmid for transformation? - (reply: 8)
42. RE digestion and ligation troubleshooting. - (reply: 1)
43. replicating a mammalian expression vector in bacteria - (reply: 3)
44. Cloning of an unknown gene - (reply: 3)
45. cloning problems using in-fusion kit - (reply: 5)
46. Mutation in one chain of homodimer - (reply: 4)
47. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
48. Alternative competent cells for QuikChange kit - (reply: 2)
49. Ligation Mistake - (reply: 4)
50. Cloning - Ligation problem - (reply: 3)
51. How to clone a 2A peptide between two ORFs? - (reply: 2)
52. preparing a vector for transfection - (reply: 2)
53. Ligation of two PCR products - (reply: 1)
54. TA Cloning - (reply: 11)
55. Troubleshootting lentiviral transduction. - (reply: 3)
56. Trouble with PCR using ligation mix as template? - (reply: 3)
57. Suitable ratio of vector and insert in cloning - (reply: 5)
58. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
59. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
60. plasmid repositories - (reply: 2)