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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1112. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1113. hENT1 - (reply: 1)
1114. digestion of insert twice and ligation of 3 bits - (reply: 6)
1115. blunt end ligation problem - Self ligation and truncate (reply: 1)
1116. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1117. neb ligase - (reply: 2)
1118. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1119. problem about vector Dephosphorylation - (reply: 2)
1120. Early transfection - (reply: 6)
1121. Cloning: Randomly recombined Plasmids? - (reply: 6)
1122. problem about fused protein - problem about fused protein (reply: 2)
1123. Bacteria growing on plate but not liquid media - (reply: 8)
1124. What means BamHI-linkered - (reply: 1)
1125. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1126. Problems with Dpn1 - (reply: 5)
1127. forgot to flip the plate over - but still got colonies (reply: 2)
1128. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1129. Yet another ligation problem - (reply: 6)
1130. Cloning problems - (reply: 7)
1131. Bacteria and Amaxa - (reply: 2)
1132. Problem in Ligation - (reply: 12)
1133. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1134. multiway ligation - (reply: 6)
1135. Plasmid size problems! - (reply: 3)
1136. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1137. ligation problem - (reply: 6)
1138. Failed Ligation - (reply: 2)
1139. cloning of GroEL - (reply: 3)
1140. Ligation problem - (reply: 9)