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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. forgot to flip the plate over - but still got colonies (reply: 2)
1112. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1113. Yet another ligation problem - (reply: 6)
1114. Cloning problems - (reply: 7)
1115. Bacteria and Amaxa - (reply: 2)
1116. Problem in Ligation - (reply: 12)
1117. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1118. multiway ligation - (reply: 6)
1119. Plasmid size problems! - (reply: 3)
1120. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1121. ligation problem - (reply: 6)
1122. Failed Ligation - (reply: 2)
1123. cloning of GroEL - (reply: 3)
1124. Ligation problem - (reply: 9)
1125. Problems with High Background with Gateway Cloning - (reply: 1)
1126. Mutagenesis troubles - (reply: 2)
1127. ligation/transformation failed - (reply: 7)
1128. Transfecting primary cells with plasmid - (reply: 1)
1129. Extra bands after QIAGEN spin miniprep - (reply: 3)
1130. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1131. The use of hygromycin - (reply: 2)
1132. QUESTION about cloning - (reply: 5)
1133. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1134. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1135. Ligation screening - (reply: 4)
1136. Double digestion and ligation problem - (reply: 1)
1137. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1138. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1139. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1140. CMV promoter - (reply: 2)