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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. low yeild fo RNA after invitro transcription - (reply: 4)
1112. No expresion of transgene - Retroviral vector (reply: 1)
1113. plasmids - plasmids for stable cell line construction (reply: 2)
1114. empty vectors more than the insert - (reply: 6)
1115. Restriction enzyme digestion problem - (reply: 2)
1116. low copy plasmid - how to improve the yield? (reply: 1)
1117. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1118. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1119. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1120. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1121. Cloning Help!! - It is getting very annoying... (reply: 13)
1122. restriction digestion - (reply: 7)
1123. sequence errors created during cloning - (reply: 6)
1124. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1125. the pyrithiamine - (reply: 5)
1126. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1127. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1128. What is the role of DMSO in yeast transformation - (reply: 1)
1129. ligation problem - (reply: 3)
1130. After Transfection - (reply: 1)
1131. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1132. Help cloning large insert into large vector - (reply: 7)
1133. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1134. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1135. hENT1 - (reply: 1)
1136. digestion of insert twice and ligation of 3 bits - (reply: 6)
1137. blunt end ligation problem - Self ligation and truncate (reply: 1)
1138. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1139. neb ligase - (reply: 2)
1140. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)