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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. problems with qiagen gel extraction kit - (reply: 7)
1112. restriction enzyme flanking sequence preferance - (reply: 2)
1113. pCDH Lentivector has 500 bp insert - (reply: 5)
1114. 5' RACE for very GC-rich gene - (reply: 2)
1115. Phosphorylated primers - (reply: 5)
1116. Correct orientation of the insert - (reply: 7)
1117. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1118. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1119. Cloning advice needed - (reply: 4)
1120. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1121. Colony Screeing without using PCR - (reply: 8)
1122. blue white screening - (reply: 3)
1123. phosphorylate dprimers for pfx do not work - (reply: 3)
1124. help - (reply: 1)
1125. problem with yeast transformation - (reply: 2)
1126. ta cloning of pfu amplified fragment - (reply: 9)
1127. how to express the gene in opposite transcriptional direction - (reply: 3)
1128. Water for electrocompetent cells - (reply: 2)
1129. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1130. Bacterial growth in agar plate with ampicillin - (reply: 4)
1131. problem in ligation and cloning - (reply: 1)
1132. Electrophoresis gel boxes and geldoc system - (reply: 2)
1133. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1134. problem with 293T cell co-transfection - (reply: 5)
1135. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1136. how CIP treatment is done - (reply: 3)
1137. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1138. Storage of gel extracted DNA before ligation - (reply: 3)
1139. Dual Luciferase Assay - (reply: 3)
1140. Ligation Problems - (reply: 2)