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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1112. hENT1 - (reply: 1)
1113. digestion of insert twice and ligation of 3 bits - (reply: 6)
1114. blunt end ligation problem - Self ligation and truncate (reply: 1)
1115. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1116. neb ligase - (reply: 2)
1117. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1118. problem about vector Dephosphorylation - (reply: 2)
1119. Early transfection - (reply: 6)
1120. Cloning: Randomly recombined Plasmids? - (reply: 6)
1121. problem about fused protein - problem about fused protein (reply: 2)
1122. Bacteria growing on plate but not liquid media - (reply: 8)
1123. What means BamHI-linkered - (reply: 1)
1124. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1125. Problems with Dpn1 - (reply: 5)
1126. forgot to flip the plate over - but still got colonies (reply: 2)
1127. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1128. Yet another ligation problem - (reply: 6)
1129. Cloning problems - (reply: 7)
1130. Bacteria and Amaxa - (reply: 2)
1131. Problem in Ligation - (reply: 12)
1132. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1133. multiway ligation - (reply: 6)
1134. Plasmid size problems! - (reply: 3)
1135. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1136. ligation problem - (reply: 6)
1137. Failed Ligation - (reply: 2)
1138. cloning of GroEL - (reply: 3)
1139. Ligation problem - (reply: 9)
1140. Problems with High Background with Gateway Cloning - (reply: 1)