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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Histogram from Nanodrop - (reply: 1)
1112. cDNA library construction - I get only one colony (reply: 2)
1113. Cloning into pIBV5-His (not working) - (reply: 1)
1114. Addition of Restriction sites into PCR primers - (reply: 4)
1115. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1116. Streaky DNA digest - (reply: 3)
1117. TOPO TA cloning problem - (reply: 12)
1118. cloning problem - (reply: 2)
1119. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1120. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1121. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1122. Nco1 digest problem - (reply: 4)
1123. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1124. ligation - (reply: 1)
1125. pre-prepararation of competent cells - (reply: 3)
1126. different copy # multimers with linker ligation? - (reply: 2)
1127. Help ADD 3 bases to DNA Sequences - (reply: 6)
1128. My battle with BamHI - (reply: 34)
1129. serious problem in cloning - (reply: 2)
1130. What are "Ella-cre" mice? - (reply: 2)
1131. help with pCMV-myc vector - (reply: 2)
1132. Elution amount - (reply: 5)
1133. keeping restriction digested fragments at -20? - (reply: 5)
1134. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1135. re-digestion - (reply: 1)
1136. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1137. Cloning using BamH1 and HindIII - (reply: 8)
1138. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1139. "competentcy" of my method - (reply: 5)
1140. Vector frameshift problem - (reply: 3)