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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Extra bands after QIAGEN spin miniprep - (reply: 3)
1112. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1113. The use of hygromycin - (reply: 2)
1114. QUESTION about cloning - (reply: 5)
1115. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1116. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1117. Ligation screening - (reply: 4)
1118. Double digestion and ligation problem - (reply: 1)
1119. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1120. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1121. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1122. CMV promoter - (reply: 2)
1123. insert in the reverse orientation after transformation - (reply: 5)
1124. cDNA as PCR template - (reply: 1)
1125. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1126. T4 ligase buffer - (reply: 4)
1127. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1128. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1129. ligation problems - no colonies at the end!!!!! (reply: 4)
1130. Good stable mammalian promoter - (reply: 1)
1131. Self-ligation from a Double Digest - (reply: 4)
1132. Strange frameshift question - (reply: 2)
1133. Plasmid DNA mini-prep - no good isolation (reply: 5)
1134. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1135. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1136. BstUI digestion - (reply: 1)
1137. I have cloning problems with pGEM-T Easy - (reply: 5)
1138. CaCl2 for making competent cells - (reply: 3)
1139. best way to transport bacteria at room temperature - (reply: 5)
1140. Suicide vector - (reply: 2)