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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1112. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1113. mammalian expression vector - (reply: 2)
1114. Electroporation doesn't like my plasmid??? - (reply: 4)
1115. low yeild fo RNA after invitro transcription - (reply: 4)
1116. No expresion of transgene - Retroviral vector (reply: 1)
1117. plasmids - plasmids for stable cell line construction (reply: 2)
1118. empty vectors more than the insert - (reply: 6)
1119. Restriction enzyme digestion problem - (reply: 2)
1120. low copy plasmid - how to improve the yield? (reply: 1)
1121. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1122. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1123. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1124. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1125. Cloning Help!! - It is getting very annoying... (reply: 13)
1126. restriction digestion - (reply: 7)
1127. sequence errors created during cloning - (reply: 6)
1128. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1129. the pyrithiamine - (reply: 5)
1130. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1131. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1132. What is the role of DMSO in yeast transformation - (reply: 1)
1133. ligation problem - (reply: 3)
1134. After Transfection - (reply: 1)
1135. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1136. Help cloning large insert into large vector - (reply: 7)
1137. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1138. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1139. hENT1 - (reply: 1)
1140. digestion of insert twice and ligation of 3 bits - (reply: 6)