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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Trouble with subcloning - (reply: 6)
1112. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1113. Lb-kan plates life - How long can they be used? (reply: 2)
1114. LR reaction - Cloning into a expression vector (reply: 1)
1115. TOPO-Transformation Problem - (reply: 4)
1116. Checking Plasmid Expression - (reply: 1)
1117. designing primers for genes not sequenced yet - (reply: 2)
1118. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1119. Cloning Problem - (reply: 3)
1120. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1121. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1122. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1123. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1124. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1125. Restriction buffer composition - (reply: 2)
1126. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1127. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1128. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1129. Yet another ligation problem - (reply: 4)
1130. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1131. Promoter Cloning for Luciferase Assay - (reply: 3)
1132. Creating mutants - (reply: 3)
1133. cloning protocol - (reply: 1)
1134. Ligation Time and Temp - (reply: 4)
1135. Miniprep - (reply: 3)
1136. PCR and then ligation - (reply: 7)
1137. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1138. 3 way ligation help needed - (reply: 10)
1139. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1140. Cloning woes - Running out of ideas! - (reply: 7)