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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1112. What is the role of DMSO in yeast transformation - (reply: 1)
1113. ligation problem - (reply: 3)
1114. After Transfection - (reply: 1)
1115. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1116. Help cloning large insert into large vector - (reply: 7)
1117. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1118. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1119. hENT1 - (reply: 1)
1120. digestion of insert twice and ligation of 3 bits - (reply: 6)
1121. blunt end ligation problem - Self ligation and truncate (reply: 1)
1122. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1123. neb ligase - (reply: 2)
1124. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1125. problem about vector Dephosphorylation - (reply: 2)
1126. Early transfection - (reply: 6)
1127. Cloning: Randomly recombined Plasmids? - (reply: 6)
1128. problem about fused protein - problem about fused protein (reply: 2)
1129. Bacteria growing on plate but not liquid media - (reply: 8)
1130. What means BamHI-linkered - (reply: 1)
1131. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1132. Problems with Dpn1 - (reply: 5)
1133. forgot to flip the plate over - but still got colonies (reply: 2)
1134. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1135. Yet another ligation problem - (reply: 6)
1136. Cloning problems - (reply: 7)
1137. Bacteria and Amaxa - (reply: 2)
1138. Problem in Ligation - (reply: 12)
1139. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1140. multiway ligation - (reply: 6)