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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Screening colonies after transformation - (reply: 2)
1112. z competent cells - (reply: 2)
1113. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1114. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1115. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1116. plasmid extraction kit wash buffer solution - (reply: 3)
1117. molecular "terminology" - (reply: 1)
1118. NdeI digestion - Share your experience (reply: 3)
1119. Multiple bands after amping insert - (reply: 2)
1120. Good tool for sequence validation? - For Windows (reply: 2)
1121. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1122. Silencing of the first cistron - (reply: 1)
1123. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1124. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1125. Stable Mammalian Expression with Retrovirus - (reply: 2)
1126. Neon Transfection System - (reply: 2)
1127. help - (reply: 5)
1128. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1129. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1130. problems with qiagen gel extraction kit - (reply: 7)
1131. restriction enzyme flanking sequence preferance - (reply: 2)
1132. pCDH Lentivector has 500 bp insert - (reply: 5)
1133. 5' RACE for very GC-rich gene - (reply: 2)
1134. Phosphorylated primers - (reply: 5)
1135. Correct orientation of the insert - (reply: 7)
1136. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1137. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1138. Cloning advice needed - (reply: 4)
1139. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1140. Colony Screeing without using PCR - (reply: 8)