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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1112. Cloning using BamH1 and HindIII - (reply: 8)
1113. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1114. "competentcy" of my method - (reply: 5)
1115. Vector frameshift problem - (reply: 3)
1116. Screening colonies after transformation - (reply: 2)
1117. z competent cells - (reply: 2)
1118. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1119. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1120. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1121. plasmid extraction kit wash buffer solution - (reply: 3)
1122. molecular "terminology" - (reply: 1)
1123. NdeI digestion - Share your experience (reply: 3)
1124. Multiple bands after amping insert - (reply: 2)
1125. Good tool for sequence validation? - For Windows (reply: 2)
1126. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1127. Silencing of the first cistron - (reply: 1)
1128. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1129. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1130. Stable Mammalian Expression with Retrovirus - (reply: 2)
1131. Neon Transfection System - (reply: 2)
1132. help - (reply: 5)
1133. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1134. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1135. problems with qiagen gel extraction kit - (reply: 7)
1136. restriction enzyme flanking sequence preferance - (reply: 2)
1137. pCDH Lentivector has 500 bp insert - (reply: 5)
1138. 5' RACE for very GC-rich gene - (reply: 2)
1139. Phosphorylated primers - (reply: 5)
1140. Correct orientation of the insert - (reply: 7)