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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Problems with High Background with Gateway Cloning - (reply: 1)
1112. Mutagenesis troubles - (reply: 2)
1113. ligation/transformation failed - (reply: 7)
1114. Transfecting primary cells with plasmid - (reply: 1)
1115. Extra bands after QIAGEN spin miniprep - (reply: 3)
1116. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1117. The use of hygromycin - (reply: 2)
1118. QUESTION about cloning - (reply: 5)
1119. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1120. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1121. Ligation screening - (reply: 4)
1122. Double digestion and ligation problem - (reply: 1)
1123. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1124. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1125. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1126. CMV promoter - (reply: 2)
1127. insert in the reverse orientation after transformation - (reply: 5)
1128. cDNA as PCR template - (reply: 1)
1129. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1130. T4 ligase buffer - (reply: 4)
1131. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1132. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1133. ligation problems - no colonies at the end!!!!! (reply: 4)
1134. Good stable mammalian promoter - (reply: 1)
1135. Self-ligation from a Double Digest - (reply: 4)
1136. Strange frameshift question - (reply: 2)
1137. Plasmid DNA mini-prep - no good isolation (reply: 5)
1138. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1139. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1140. BstUI digestion - (reply: 1)