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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Electroporation doesn't like my plasmid??? - (reply: 4)
1112. low yeild fo RNA after invitro transcription - (reply: 4)
1113. No expresion of transgene - Retroviral vector (reply: 1)
1114. plasmids - plasmids for stable cell line construction (reply: 2)
1115. empty vectors more than the insert - (reply: 6)
1116. Restriction enzyme digestion problem - (reply: 2)
1117. low copy plasmid - how to improve the yield? (reply: 1)
1118. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1119. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1120. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1121. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1122. Cloning Help!! - It is getting very annoying... (reply: 13)
1123. restriction digestion - (reply: 7)
1124. sequence errors created during cloning - (reply: 6)
1125. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1126. the pyrithiamine - (reply: 5)
1127. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1128. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1129. What is the role of DMSO in yeast transformation - (reply: 1)
1130. ligation problem - (reply: 3)
1131. After Transfection - (reply: 1)
1132. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1133. Help cloning large insert into large vector - (reply: 7)
1134. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1135. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1136. hENT1 - (reply: 1)
1137. digestion of insert twice and ligation of 3 bits - (reply: 6)
1138. blunt end ligation problem - Self ligation and truncate (reply: 1)
1139. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1140. neb ligase - (reply: 2)