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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1112. T4 ligase buffer - (reply: 4)
1113. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1114. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1115. ligation problems - no colonies at the end!!!!! (reply: 4)
1116. Good stable mammalian promoter - (reply: 1)
1117. Self-ligation from a Double Digest - (reply: 4)
1118. Strange frameshift question - (reply: 2)
1119. Plasmid DNA mini-prep - no good isolation (reply: 5)
1120. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1121. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1122. BstUI digestion - (reply: 1)
1123. I have cloning problems with pGEM-T Easy - (reply: 5)
1124. CaCl2 for making competent cells - (reply: 3)
1125. best way to transport bacteria at room temperature - (reply: 5)
1126. Suicide vector - (reply: 2)
1127. Extremely small bacterial colonies after transformation - (reply: 10)
1128. tranformation efficiency - (reply: 2)
1129. transfection time - (reply: 2)
1130. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1131. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1132. Problem with SmaI restriction digest - (reply: 6)
1133. blunt-end / 3ŽA overhang cloning - (reply: 1)
1134. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1135. overexpression of stable clones - (reply: 1)
1136. functionality of yeast promoter in E. coli - (reply: 1)
1137. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1138. Can't seem to do a simple cloning - (reply: 2)
1139. Histogram from Nanodrop - (reply: 1)
1140. cDNA library construction - I get only one colony (reply: 2)