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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1112. the pyrithiamine - (reply: 5)
1113. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1114. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1115. What is the role of DMSO in yeast transformation - (reply: 1)
1116. ligation problem - (reply: 3)
1117. After Transfection - (reply: 1)
1118. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1119. Help cloning large insert into large vector - (reply: 7)
1120. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1121. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1122. hENT1 - (reply: 1)
1123. digestion of insert twice and ligation of 3 bits - (reply: 6)
1124. blunt end ligation problem - Self ligation and truncate (reply: 1)
1125. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1126. neb ligase - (reply: 2)
1127. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1128. problem about vector Dephosphorylation - (reply: 2)
1129. Early transfection - (reply: 6)
1130. Cloning: Randomly recombined Plasmids? - (reply: 6)
1131. problem about fused protein - problem about fused protein (reply: 2)
1132. Bacteria growing on plate but not liquid media - (reply: 8)
1133. What means BamHI-linkered - (reply: 1)
1134. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1135. Problems with Dpn1 - (reply: 5)
1136. forgot to flip the plate over - but still got colonies (reply: 2)
1137. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1138. Yet another ligation problem - (reply: 6)
1139. Cloning problems - (reply: 7)
1140. Bacteria and Amaxa - (reply: 2)