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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. blunt-end / 3ŽA overhang cloning - (reply: 1)
1112. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1113. overexpression of stable clones - (reply: 1)
1114. functionality of yeast promoter in E. coli - (reply: 1)
1115. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1116. Can't seem to do a simple cloning - (reply: 2)
1117. Histogram from Nanodrop - (reply: 1)
1118. cDNA library construction - I get only one colony (reply: 2)
1119. Cloning into pIBV5-His (not working) - (reply: 1)
1120. Addition of Restriction sites into PCR primers - (reply: 4)
1121. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1122. Streaky DNA digest - (reply: 3)
1123. TOPO TA cloning problem - (reply: 12)
1124. cloning problem - (reply: 2)
1125. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1126. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1127. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1128. Nco1 digest problem - (reply: 4)
1129. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1130. ligation - (reply: 1)
1131. pre-prepararation of competent cells - (reply: 3)
1132. different copy # multimers with linker ligation? - (reply: 2)
1133. Help ADD 3 bases to DNA Sequences - (reply: 6)
1134. My battle with BamHI - (reply: 34)
1135. serious problem in cloning - (reply: 2)
1136. What are "Ella-cre" mice? - (reply: 2)
1137. help with pCMV-myc vector - (reply: 2)
1138. Elution amount - (reply: 5)
1139. keeping restriction digested fragments at -20? - (reply: 5)
1140. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)