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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1112. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1113. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1114. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1115. Cloning Help!! - It is getting very annoying... (reply: 13)
1116. restriction digestion - (reply: 7)
1117. sequence errors created during cloning - (reply: 6)
1118. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1119. the pyrithiamine - (reply: 5)
1120. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1121. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1122. What is the role of DMSO in yeast transformation - (reply: 1)
1123. ligation problem - (reply: 3)
1124. After Transfection - (reply: 1)
1125. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1126. Help cloning large insert into large vector - (reply: 7)
1127. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1128. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1129. hENT1 - (reply: 1)
1130. digestion of insert twice and ligation of 3 bits - (reply: 6)
1131. blunt end ligation problem - Self ligation and truncate (reply: 1)
1132. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1133. neb ligase - (reply: 2)
1134. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1135. problem about vector Dephosphorylation - (reply: 2)
1136. Early transfection - (reply: 6)
1137. Cloning: Randomly recombined Plasmids? - (reply: 6)
1138. problem about fused protein - problem about fused protein (reply: 2)
1139. Bacteria growing on plate but not liquid media - (reply: 8)
1140. What means BamHI-linkered - (reply: 1)