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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1112. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1113. What is the role of DMSO in yeast transformation - (reply: 1)
1114. ligation problem - (reply: 3)
1115. After Transfection - (reply: 1)
1116. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1117. Help cloning large insert into large vector - (reply: 7)
1118. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1119. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1120. hENT1 - (reply: 1)
1121. digestion of insert twice and ligation of 3 bits - (reply: 6)
1122. blunt end ligation problem - Self ligation and truncate (reply: 1)
1123. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1124. neb ligase - (reply: 2)
1125. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1126. problem about vector Dephosphorylation - (reply: 2)
1127. Early transfection - (reply: 6)
1128. Cloning: Randomly recombined Plasmids? - (reply: 6)
1129. problem about fused protein - problem about fused protein (reply: 2)
1130. Bacteria growing on plate but not liquid media - (reply: 8)
1131. What means BamHI-linkered - (reply: 1)
1132. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1133. Problems with Dpn1 - (reply: 5)
1134. forgot to flip the plate over - but still got colonies (reply: 2)
1135. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1136. Yet another ligation problem - (reply: 6)
1137. Cloning problems - (reply: 7)
1138. Bacteria and Amaxa - (reply: 2)
1139. Problem in Ligation - (reply: 12)
1140. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)