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Top : New Forum Archives (2009-): : Molecular-Cloning
1111. CaCl2 for making competent cells - (reply: 3)
1112. best way to transport bacteria at room temperature - (reply: 5)
1113. Suicide vector - (reply: 2)
1114. Extremely small bacterial colonies after transformation - (reply: 10)
1115. tranformation efficiency - (reply: 2)
1116. transfection time - (reply: 2)
1117. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1118. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1119. Problem with SmaI restriction digest - (reply: 6)
1120. blunt-end / 3ŽA overhang cloning - (reply: 1)
1121. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1122. overexpression of stable clones - (reply: 1)
1123. functionality of yeast promoter in E. coli - (reply: 1)
1124. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1125. Can't seem to do a simple cloning - (reply: 2)
1126. Histogram from Nanodrop - (reply: 1)
1127. cDNA library construction - I get only one colony (reply: 2)
1128. Cloning into pIBV5-His (not working) - (reply: 1)
1129. Addition of Restriction sites into PCR primers - (reply: 4)
1130. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1131. Streaky DNA digest - (reply: 3)
1132. TOPO TA cloning problem - (reply: 12)
1133. cloning problem - (reply: 2)
1134. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1135. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1136. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1137. Nco1 digest problem - (reply: 4)
1138. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1139. ligation - (reply: 1)
1140. pre-prepararation of competent cells - (reply: 3)