Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1111. ligation - (reply: 1)
1112. pre-prepararation of competent cells - (reply: 3)
1113. different copy # multimers with linker ligation? - (reply: 2)
1114. Help ADD 3 bases to DNA Sequences - (reply: 6)
1115. My battle with BamHI - (reply: 34)
1116. serious problem in cloning - (reply: 2)
1117. What are "Ella-cre" mice? - (reply: 2)
1118. help with pCMV-myc vector - (reply: 2)
1119. Elution amount - (reply: 5)
1120. keeping restriction digested fragments at -20? - (reply: 5)
1121. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1122. re-digestion - (reply: 1)
1123. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1124. Cloning using BamH1 and HindIII - (reply: 8)
1125. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1126. "competentcy" of my method - (reply: 5)
1127. Vector frameshift problem - (reply: 3)
1128. Screening colonies after transformation - (reply: 2)
1129. z competent cells - (reply: 2)
1130. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1131. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1132. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1133. plasmid extraction kit wash buffer solution - (reply: 3)
1134. molecular "terminology" - (reply: 1)
1135. NdeI digestion - Share your experience (reply: 3)
1136. Multiple bands after amping insert - (reply: 2)
1137. Good tool for sequence validation? - For Windows (reply: 2)
1138. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1139. Silencing of the first cistron - (reply: 1)
1140. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)