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361. PCR insert - in frame? - (reply: 2)
362. Whole plasmid amplification by PCR - (reply: 2)
363. colony PCR after transformation - (reply: 1)
364. For promoter assay, which vector I should use? - (reply: 5)
365. Sequencing of the cloned plasmid - (reply: 5)
366. 4 way cloning into TA Vector - (reply: 10)
367. I get band double the size what does it mean - (reply: 3)
368. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
369. Neeed Help , cloning Confirmation ?! - (reply: 5)
370. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
371. Can I quantify restricted plasmid and oligos? - (reply: 3)
372. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
373. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
374. TOPO TA ligation problems - (reply: 6)
375. Luciferase assay problem - (reply: 2)
376. Double digestion with BamHI and BglII - (reply: 4)
377. nicked linear DNA - (reply: 5)
378. Selection of E.coli competent cells - (reply: 2)
379. Double strand cdna synthysis - (reply: 4)
380. mutagenic primers with very high GC content. - (reply: 3)
381. TA Cloning of ds cDNA - (reply: 6)
382. Promoter distance from ATG - (reply: 3)
383. Measuring Fluorescene of RFP - (reply: 7)
384. Smallest possible insert size for Ligation reaction - (reply: 2)
385. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
386. Extraction of DNA from termite gut flagellates - (reply: 7)
387. Enzymes and buffers from different companies - (reply: 6)
388. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
389. faint band after plasmid extraction - (reply: 7)
390. Adaptor ligation - (reply: 9)
362. Whole plasmid amplification by PCR - (reply: 2)
363. colony PCR after transformation - (reply: 1)
364. For promoter assay, which vector I should use? - (reply: 5)
365. Sequencing of the cloned plasmid - (reply: 5)
366. 4 way cloning into TA Vector - (reply: 10)
367. I get band double the size what does it mean - (reply: 3)
368. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
369. Neeed Help , cloning Confirmation ?! - (reply: 5)
370. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
371. Can I quantify restricted plasmid and oligos? - (reply: 3)
372. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
373. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
374. TOPO TA ligation problems - (reply: 6)
375. Luciferase assay problem - (reply: 2)
376. Double digestion with BamHI and BglII - (reply: 4)
377. nicked linear DNA - (reply: 5)
378. Selection of E.coli competent cells - (reply: 2)
379. Double strand cdna synthysis - (reply: 4)
380. mutagenic primers with very high GC content. - (reply: 3)
381. TA Cloning of ds cDNA - (reply: 6)
382. Promoter distance from ATG - (reply: 3)
383. Measuring Fluorescene of RFP - (reply: 7)
384. Smallest possible insert size for Ligation reaction - (reply: 2)
385. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
386. Extraction of DNA from termite gut flagellates - (reply: 7)
387. Enzymes and buffers from different companies - (reply: 6)
388. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
389. faint band after plasmid extraction - (reply: 7)
390. Adaptor ligation - (reply: 9)