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Top : New Forum Archives (2009-): : Molecular-Cloning
361. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
362. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
363. Freeze transformation mixture? - (reply: 2)
364. Cutting my plasmid made it larger - (reply: 7)
365. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
366. Amplification of CAG promoter problems - (reply: 4)
367. restriction enzyme - (reply: 2)
368. Recreate original plasmid by cutting out insert - (reply: 2)
369. Colony-PCR workaround - (reply: 3)
370. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
371. Can I pause a bacterial culture at 4C - (reply: 2)
372. TOPO TA CLONING - LIGATION STEP - (reply: 9)
373. posting sequences of plasmid? - (reply: 3)
374. Restriction Enzyme Digest not working - (reply: 2)
375. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
376. Incomplete digestion of plasmid with single enzyme - (reply: 1)
377. problem with plasmid digestion - (reply: 5)
378. Difficult Ligation - Details Inside - (reply: 9)
379. pET 32 (+) - (reply: 1)
380. Alternative to pET Vectorsystem - (reply: 2)
381. pEGFP C-1/N-1 Cloning - (reply: 11)
382. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
383. Problems with growing out transformants in liquid LB - (reply: 3)
384. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
385. Ethidiumbromide - (reply: 3)
386. TRYPSIN - (reply: 4)
387. Problem with cloning - what is wrong? - (reply: 6)
388. cloning: band at different site than desired after RE digestion - (reply: 11)
389. GST TAG - (reply: 3)
390. Residues on the N terminal GST Tag - (reply: 1)