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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Restriction site rearrangement - (reply: 1)
362. Mutation in cloning - (reply: 6)
363. Must the terminator be in frame? - (reply: 1)
364. sequencing problems - (reply: 2)
365. Understand the basics of molecular cloning - (reply: 6)
366. Maintainence of transformed cells - (reply: 5)
367. Restriction digest band shift? - (reply: 1)
368. problem in cloning PCR primer design with restriction site - (reply: 4)
369. quick change mutagenesis...... - (reply: 1)
370. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
371. Help for pGEM-T easy vector ligation problem - (reply: 2)
372. Co-transformation - (reply: 2)
373. Problems regarding point mutations.... - (reply: 4)
374. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
375. pkp 59 vector - (reply: 2)
376. Vector insert ratio in cloning - (reply: 3)
377. polymerase to use for cloning - (reply: 4)
378. Oligo insert cloning - (reply: 3)
379. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
380. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
381. How to select competent E. coli cells? - (reply: 7)
382. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
383. cDNA amplification problem - (reply: 4)
384. Ligation: two basic questions - (reply: 1)
385. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
386. Unexpected base after deletion mutagenesis - (reply: 3)
387. Confirmation of ligation - (reply: 7)
388. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
389. Ligation of large ammount of insert (no transformation) - (reply: 7)
390. PCR and restriction enzyme digestion - (reply: 3)