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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Few colonies frm sticky end cloning - (reply: 3)
362. transformation problem - (reply: 8)
363. Problems with Digestion? - (reply: 7)
364. plasmid digestion - problem with enzymes? - (reply: 4)
365. Problem with cloning - (reply: 7)
366. double transformation problem - (reply: 3)
367. Too many negative clones using Directional TOPO Cloning - (reply: 5)
368. competent cells - (reply: 1)
369. "Easiest" sticky end combination - (reply: 3)
370. Cloning advice - (reply: 3)
371. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
372. Cloning large fragments - (reply: 9)
373. Transformation colonies does not contain insert - (reply: 3)
374. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
375. Need urgent advise.. - (reply: 3)
376. Cloning Mitochondrial Targeting Sequence - (reply: 4)
377. problem in cloning - (reply: 2)
378. TA cloning: ligation problem or toxic construct? - (reply: 4)
379. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
380. Do I need to sequence after digestion? - (reply: 9)
381. quick change mutagenesis...... - (reply: 15)
382. Problem with double digestion: one enzyme is not working - (reply: 8)
383. Transient Transfection Controls (Gateway Technology) - (reply: 4)
384. Problems with crossover PCR - (reply: 2)
385. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
386. Two genes, same MCS - (reply: 1)
387. Including ATG in cloning? - (reply: 1)
388. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
389. Low copy vector for library generation - (reply: 2)
390. Can't find a recombinant after ligating - (reply: 4)