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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
362. Extraction of DNA from termite gut flagellates - (reply: 7)
363. Enzymes and buffers from different companies - (reply: 6)
364. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
365. faint band after plasmid extraction - (reply: 7)
366. Adaptor ligation - (reply: 9)
367. self ligation - (reply: 1)
368. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
369. Cloning large transgenes - (reply: 2)
370. In-fusion HD cloning - (reply: 5)
371. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
372. Few colonies frm sticky end cloning - (reply: 3)
373. transformation problem - (reply: 8)
374. Problems with Digestion? - (reply: 7)
375. plasmid digestion - problem with enzymes? - (reply: 4)
376. Problem with cloning - (reply: 7)
377. double transformation problem - (reply: 3)
378. Too many negative clones using Directional TOPO Cloning - (reply: 5)
379. competent cells - (reply: 1)
380. "Easiest" sticky end combination - (reply: 3)
381. Cloning advice - (reply: 3)
382. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
383. Cloning large fragments - (reply: 9)
384. Transformation colonies does not contain insert - (reply: 3)
385. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
386. Need urgent advise.. - (reply: 3)
387. Cloning Mitochondrial Targeting Sequence - (reply: 4)
388. problem in cloning - (reply: 2)
389. TA cloning: ligation problem or toxic construct? - (reply: 4)
390. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)