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Top : New Forum Archives (2009-): : Molecular-Cloning
361. What is NEB's CutSmart Buffer? - (reply: 5)
362. Enzyme cutting: low efficiency - (reply: 5)
363. Issue with Xba1 and Dam methylation - (reply: 7)
364. Restriction site rearrangement - (reply: 1)
365. Mutation in cloning - (reply: 6)
366. Must the terminator be in frame? - (reply: 1)
367. sequencing problems - (reply: 2)
368. Understand the basics of molecular cloning - (reply: 6)
369. Maintainence of transformed cells - (reply: 5)
370. Restriction digest band shift? - (reply: 1)
371. problem in cloning PCR primer design with restriction site - (reply: 4)
372. quick change mutagenesis...... - (reply: 1)
373. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
374. Help for pGEM-T easy vector ligation problem - (reply: 2)
375. Co-transformation - (reply: 2)
376. Problems regarding point mutations.... - (reply: 4)
377. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
378. pkp 59 vector - (reply: 2)
379. Vector insert ratio in cloning - (reply: 3)
380. polymerase to use for cloning - (reply: 4)
381. Oligo insert cloning - (reply: 3)
382. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
383. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
384. How to select competent E. coli cells? - (reply: 7)
385. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
386. cDNA amplification problem - (reply: 4)
387. Ligation: two basic questions - (reply: 1)
388. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
389. Unexpected base after deletion mutagenesis - (reply: 3)
390. Confirmation of ligation - (reply: 7)