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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
362. faint band after plasmid extraction - (reply: 7)
363. Adaptor ligation - (reply: 9)
364. self ligation - (reply: 1)
365. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
366. Cloning large transgenes - (reply: 2)
367. In-fusion HD cloning - (reply: 5)
368. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
369. Few colonies frm sticky end cloning - (reply: 3)
370. transformation problem - (reply: 8)
371. Problems with Digestion? - (reply: 7)
372. plasmid digestion - problem with enzymes? - (reply: 4)
373. Problem with cloning - (reply: 7)
374. double transformation problem - (reply: 3)
375. Too many negative clones using Directional TOPO Cloning - (reply: 5)
376. competent cells - (reply: 1)
377. "Easiest" sticky end combination - (reply: 3)
378. Cloning advice - (reply: 3)
379. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
380. Cloning large fragments - (reply: 9)
381. Transformation colonies does not contain insert - (reply: 3)
382. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
383. Need urgent advise.. - (reply: 3)
384. Cloning Mitochondrial Targeting Sequence - (reply: 4)
385. problem in cloning - (reply: 2)
386. TA cloning: ligation problem or toxic construct? - (reply: 4)
387. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
388. Do I need to sequence after digestion? - (reply: 9)
389. quick change mutagenesis...... - (reply: 15)
390. Problem with double digestion: one enzyme is not working - (reply: 8)