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Top : New Forum Archives (2009-): : Molecular-Cloning
361. "Easiest" sticky end combination - (reply: 3)
362. Cloning advice - (reply: 3)
363. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
364. Cloning large fragments - (reply: 9)
365. Transformation colonies does not contain insert - (reply: 3)
366. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
367. Need urgent advise.. - (reply: 3)
368. Cloning Mitochondrial Targeting Sequence - (reply: 4)
369. problem in cloning - (reply: 2)
370. TA cloning: ligation problem or toxic construct? - (reply: 4)
371. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
372. Do I need to sequence after digestion? - (reply: 9)
373. quick change mutagenesis...... - (reply: 15)
374. Problem with double digestion: one enzyme is not working - (reply: 8)
375. Transient Transfection Controls (Gateway Technology) - (reply: 4)
376. Problems with crossover PCR - (reply: 2)
377. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
378. Two genes, same MCS - (reply: 1)
379. Including ATG in cloning? - (reply: 1)
380. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
381. Low copy vector for library generation - (reply: 2)
382. Can't find a recombinant after ligating - (reply: 4)
383. problems with plasmid miniprep - (reply: 8)
384. gene cloning - (reply: 1)
385. Is ligation really necessary? - (reply: 6)
386. problem with ligation of linker/adaptor - (reply: 2)
387. GFP -weak signal -why? - (reply: 1)
388. Ligation troubleshooting! Please help! - (reply: 5)
389. Truncated gene Cloning - (reply: 1)
390. Screening for ligation and transformation result - (reply: 2)