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Top : New Forum Archives (2009-): : Molecular-Cloning
361. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
362. Neeed Help , cloning Confirmation ?! - (reply: 5)
363. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
364. Can I quantify restricted plasmid and oligos? - (reply: 3)
365. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
366. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
367. TOPO TA ligation problems - (reply: 6)
368. Luciferase assay problem - (reply: 2)
369. Double digestion with BamHI and BglII - (reply: 4)
370. nicked linear DNA - (reply: 5)
371. Selection of E.coli competent cells - (reply: 2)
372. Double strand cdna synthysis - (reply: 4)
373. mutagenic primers with very high GC content. - (reply: 3)
374. TA Cloning of ds cDNA - (reply: 6)
375. Promoter distance from ATG - (reply: 3)
376. Measuring Fluorescene of RFP - (reply: 7)
377. Smallest possible insert size for Ligation reaction - (reply: 2)
378. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
379. Extraction of DNA from termite gut flagellates - (reply: 7)
380. Enzymes and buffers from different companies - (reply: 6)
381. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
382. faint band after plasmid extraction - (reply: 7)
383. Adaptor ligation - (reply: 9)
384. self ligation - (reply: 1)
385. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
386. Cloning large transgenes - (reply: 2)
387. In-fusion HD cloning - (reply: 5)
388. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
389. Few colonies frm sticky end cloning - (reply: 3)
390. transformation problem - (reply: 8)