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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Double digestion with BamHI and BglII - (reply: 4)
362. nicked linear DNA - (reply: 5)
363. Selection of E.coli competent cells - (reply: 2)
364. Double strand cdna synthysis - (reply: 4)
365. mutagenic primers with very high GC content. - (reply: 3)
366. TA Cloning of ds cDNA - (reply: 6)
367. Promoter distance from ATG - (reply: 3)
368. Measuring Fluorescene of RFP - (reply: 7)
369. Smallest possible insert size for Ligation reaction - (reply: 2)
370. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
371. Extraction of DNA from termite gut flagellates - (reply: 7)
372. Enzymes and buffers from different companies - (reply: 6)
373. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
374. faint band after plasmid extraction - (reply: 7)
375. Adaptor ligation - (reply: 9)
376. self ligation - (reply: 1)
377. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
378. Cloning large transgenes - (reply: 2)
379. In-fusion HD cloning - (reply: 5)
380. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
381. Few colonies frm sticky end cloning - (reply: 3)
382. transformation problem - (reply: 8)
383. Problems with Digestion? - (reply: 7)
384. plasmid digestion - problem with enzymes? - (reply: 4)
385. Problem with cloning - (reply: 7)
386. double transformation problem - (reply: 3)
387. Too many negative clones using Directional TOPO Cloning - (reply: 5)
388. competent cells - (reply: 1)
389. "Easiest" sticky end combination - (reply: 3)
390. Cloning advice - (reply: 3)