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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Extraction of DNA from termite gut flagellates - (reply: 7)
362. Enzymes and buffers from different companies - (reply: 6)
363. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
364. faint band after plasmid extraction - (reply: 7)
365. Adaptor ligation - (reply: 9)
366. self ligation - (reply: 1)
367. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
368. Cloning large transgenes - (reply: 2)
369. In-fusion HD cloning - (reply: 5)
370. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
371. Few colonies frm sticky end cloning - (reply: 3)
372. transformation problem - (reply: 8)
373. Problems with Digestion? - (reply: 7)
374. plasmid digestion - problem with enzymes? - (reply: 4)
375. Problem with cloning - (reply: 7)
376. double transformation problem - (reply: 3)
377. Too many negative clones using Directional TOPO Cloning - (reply: 5)
378. competent cells - (reply: 1)
379. "Easiest" sticky end combination - (reply: 3)
380. Cloning advice - (reply: 3)
381. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
382. Cloning large fragments - (reply: 9)
383. Transformation colonies does not contain insert - (reply: 3)
384. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
385. Need urgent advise.. - (reply: 3)
386. Cloning Mitochondrial Targeting Sequence - (reply: 4)
387. problem in cloning - (reply: 2)
388. TA cloning: ligation problem or toxic construct? - (reply: 4)
389. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
390. Do I need to sequence after digestion? - (reply: 9)