Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
361. pEGFP C-1/N-1 Cloning - (reply: 11)
362. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
363. Problems with growing out transformants in liquid LB - (reply: 3)
364. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
365. Ethidiumbromide - (reply: 3)
366. TRYPSIN - (reply: 4)
367. Problem with cloning - what is wrong? - (reply: 6)
368. cloning: band at different site than desired after RE digestion - (reply: 11)
369. GST TAG - (reply: 3)
370. Residues on the N terminal GST Tag - (reply: 1)
371. Where to place the polyA signal? - (reply: 1)
372. identification of target sequence - (reply: 3)
373. cloning pcr insert into plasmid - (reply: 2)
374. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
375. Large quantity heat shock trouble? - (reply: 3)
376. molecular cloning - (reply: 7)
377. Cannot see insert with colony pcr - (reply: 6)
378. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
379. help with a vector - (reply: 3)
380. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
381. Another cloning hell....no transformants - (reply: 1)
382. Agrobacterium LBA4404 transformation - (reply: 1)
383. TOPO TA Cloning Troubleshooting - (reply: 1)
384. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
385. Tricistronic vector - (reply: 1)
386. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
387. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
388. Directions of genes expression illustrations - (reply: 5)
389. Plasmid DNA at RT for a few days - (reply: 4)
390. TOPO TA Cloning - (reply: 1)