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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
362. Unexpected base after deletion mutagenesis - (reply: 3)
363. Confirmation of ligation - (reply: 7)
364. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
365. Ligation of large ammount of insert (no transformation) - (reply: 7)
366. PCR and restriction enzyme digestion - (reply: 3)
367. Probelm with double digestion - (reply: 1)
368. Cloning not working, all steps checked, clueless - (reply: 1)
369. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
370. recombinant protein form - (reply: 2)
371. restricted PCR plasmid runs slower - (reply: 2)
372. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
373. High frequency of vector religation - (reply: 10)
374. Cloned but not expressing! - (reply: 8)
375. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
376. DNA ligation and trasformation - (reply: 6)
377. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
378. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
379. How to set up simultaneous digestion? - (reply: 3)
380. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
381. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
382. Freeze transformation mixture? - (reply: 2)
383. Cutting my plasmid made it larger - (reply: 7)
384. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
385. Amplification of CAG promoter problems - (reply: 4)
386. restriction enzyme - (reply: 2)
387. Recreate original plasmid by cutting out insert - (reply: 2)
388. Colony-PCR workaround - (reply: 3)
389. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
390. Can I pause a bacterial culture at 4C - (reply: 2)