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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
362. Need urgent advise.. - (reply: 3)
363. Cloning Mitochondrial Targeting Sequence - (reply: 4)
364. problem in cloning - (reply: 2)
365. TA cloning: ligation problem or toxic construct? - (reply: 4)
366. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
367. Do I need to sequence after digestion? - (reply: 9)
368. quick change mutagenesis...... - (reply: 15)
369. Problem with double digestion: one enzyme is not working - (reply: 8)
370. Transient Transfection Controls (Gateway Technology) - (reply: 4)
371. Problems with crossover PCR - (reply: 2)
372. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
373. Two genes, same MCS - (reply: 1)
374. Including ATG in cloning? - (reply: 1)
375. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
376. Low copy vector for library generation - (reply: 2)
377. Can't find a recombinant after ligating - (reply: 4)
378. problems with plasmid miniprep - (reply: 8)
379. gene cloning - (reply: 1)
380. Is ligation really necessary? - (reply: 6)
381. problem with ligation of linker/adaptor - (reply: 2)
382. GFP -weak signal -why? - (reply: 1)
383. Ligation troubleshooting! Please help! - (reply: 5)
384. Truncated gene Cloning - (reply: 1)
385. Screening for ligation and transformation result - (reply: 2)
386. Problem with 3 way ligation - (reply: 2)
387. Query regarding primers for quick change mutagenesis - (reply: 3)
388. What is NEB's CutSmart Buffer? - (reply: 5)
389. Enzyme cutting: low efficiency - (reply: 5)
390. Issue with Xba1 and Dam methylation - (reply: 7)