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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Smallest possible insert size for Ligation reaction - (reply: 2)
362. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
363. Extraction of DNA from termite gut flagellates - (reply: 7)
364. Enzymes and buffers from different companies - (reply: 6)
365. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
366. faint band after plasmid extraction - (reply: 7)
367. Adaptor ligation - (reply: 9)
368. self ligation - (reply: 1)
369. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
370. Cloning large transgenes - (reply: 2)
371. In-fusion HD cloning - (reply: 5)
372. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
373. Few colonies frm sticky end cloning - (reply: 3)
374. transformation problem - (reply: 8)
375. Problems with Digestion? - (reply: 7)
376. plasmid digestion - problem with enzymes? - (reply: 4)
377. Problem with cloning - (reply: 7)
378. double transformation problem - (reply: 3)
379. Too many negative clones using Directional TOPO Cloning - (reply: 5)
380. competent cells - (reply: 1)
381. "Easiest" sticky end combination - (reply: 3)
382. Cloning advice - (reply: 3)
383. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
384. Cloning large fragments - (reply: 9)
385. Transformation colonies does not contain insert - (reply: 3)
386. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
387. Need urgent advise.. - (reply: 3)
388. Cloning Mitochondrial Targeting Sequence - (reply: 4)
389. problem in cloning - (reply: 2)
390. TA cloning: ligation problem or toxic construct? - (reply: 4)