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Top : New Forum Archives (2009-): : Molecular-Cloning
361. TOPO TA ligation problems - (reply: 6)
362. Luciferase assay problem - (reply: 2)
363. Double digestion with BamHI and BglII - (reply: 4)
364. nicked linear DNA - (reply: 5)
365. Selection of E.coli competent cells - (reply: 2)
366. Double strand cdna synthysis - (reply: 4)
367. mutagenic primers with very high GC content. - (reply: 3)
368. TA Cloning of ds cDNA - (reply: 6)
369. Promoter distance from ATG - (reply: 3)
370. Measuring Fluorescene of RFP - (reply: 7)
371. Smallest possible insert size for Ligation reaction - (reply: 2)
372. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
373. Extraction of DNA from termite gut flagellates - (reply: 7)
374. Enzymes and buffers from different companies - (reply: 6)
375. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
376. faint band after plasmid extraction - (reply: 7)
377. Adaptor ligation - (reply: 9)
378. self ligation - (reply: 1)
379. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
380. Cloning large transgenes - (reply: 2)
381. In-fusion HD cloning - (reply: 5)
382. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
383. Few colonies frm sticky end cloning - (reply: 3)
384. transformation problem - (reply: 8)
385. Problems with Digestion? - (reply: 7)
386. plasmid digestion - problem with enzymes? - (reply: 4)
387. Problem with cloning - (reply: 7)
388. double transformation problem - (reply: 3)
389. Too many negative clones using Directional TOPO Cloning - (reply: 5)
390. competent cells - (reply: 1)