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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Enzymes and buffers from different companies - (reply: 6)
362. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
363. faint band after plasmid extraction - (reply: 7)
364. Adaptor ligation - (reply: 9)
365. self ligation - (reply: 1)
366. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
367. Cloning large transgenes - (reply: 2)
368. In-fusion HD cloning - (reply: 5)
369. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
370. Few colonies frm sticky end cloning - (reply: 3)
371. transformation problem - (reply: 8)
372. Problems with Digestion? - (reply: 7)
373. plasmid digestion - problem with enzymes? - (reply: 4)
374. Problem with cloning - (reply: 7)
375. double transformation problem - (reply: 3)
376. Too many negative clones using Directional TOPO Cloning - (reply: 5)
377. competent cells - (reply: 1)
378. "Easiest" sticky end combination - (reply: 3)
379. Cloning advice - (reply: 3)
380. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
381. Cloning large fragments - (reply: 9)
382. Transformation colonies does not contain insert - (reply: 3)
383. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
384. Need urgent advise.. - (reply: 3)
385. Cloning Mitochondrial Targeting Sequence - (reply: 4)
386. problem in cloning - (reply: 2)
387. TA cloning: ligation problem or toxic construct? - (reply: 4)
388. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
389. Do I need to sequence after digestion? - (reply: 9)
390. quick change mutagenesis...... - (reply: 15)