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Top : New Forum Archives (2009-): : Molecular-Cloning
361. Difficult Ligation - Details Inside - (reply: 9)
362. pET 32 (+) - (reply: 1)
363. Alternative to pET Vectorsystem - (reply: 2)
364. pEGFP C-1/N-1 Cloning - (reply: 11)
365. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
366. Problems with growing out transformants in liquid LB - (reply: 3)
367. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
368. Ethidiumbromide - (reply: 3)
369. TRYPSIN - (reply: 4)
370. Problem with cloning - what is wrong? - (reply: 6)
371. cloning: band at different site than desired after RE digestion - (reply: 11)
372. GST TAG - (reply: 3)
373. Residues on the N terminal GST Tag - (reply: 1)
374. Where to place the polyA signal? - (reply: 1)
375. identification of target sequence - (reply: 3)
376. cloning pcr insert into plasmid - (reply: 2)
377. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
378. Large quantity heat shock trouble? - (reply: 3)
379. molecular cloning - (reply: 7)
380. Cannot see insert with colony pcr - (reply: 6)
381. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
382. help with a vector - (reply: 3)
383. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
384. Another cloning hell....no transformants - (reply: 1)
385. Agrobacterium LBA4404 transformation - (reply: 1)
386. TOPO TA Cloning Troubleshooting - (reply: 1)
387. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
388. Tricistronic vector - (reply: 1)
389. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
390. How would you find a certain tissue specific promoter for my construct? - (reply: 2)