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Top : New Forum Archives (2009-): : Molecular-Cloning
361. nicked linear DNA - (reply: 5)
362. Selection of E.coli competent cells - (reply: 2)
363. Double strand cdna synthysis - (reply: 4)
364. mutagenic primers with very high GC content. - (reply: 3)
365. TA Cloning of ds cDNA - (reply: 6)
366. Promoter distance from ATG - (reply: 3)
367. Measuring Fluorescene of RFP - (reply: 7)
368. Smallest possible insert size for Ligation reaction - (reply: 2)
369. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
370. Extraction of DNA from termite gut flagellates - (reply: 7)
371. Enzymes and buffers from different companies - (reply: 6)
372. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
373. faint band after plasmid extraction - (reply: 7)
374. Adaptor ligation - (reply: 9)
375. self ligation - (reply: 1)
376. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
377. Cloning large transgenes - (reply: 2)
378. In-fusion HD cloning - (reply: 5)
379. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
380. Few colonies frm sticky end cloning - (reply: 3)
381. transformation problem - (reply: 8)
382. Problems with Digestion? - (reply: 7)
383. plasmid digestion - problem with enzymes? - (reply: 4)
384. Problem with cloning - (reply: 7)
385. double transformation problem - (reply: 3)
386. Too many negative clones using Directional TOPO Cloning - (reply: 5)
387. competent cells - (reply: 1)
388. "Easiest" sticky end combination - (reply: 3)
389. Cloning advice - (reply: 3)
390. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)