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Top : New Forum Archives (2009-): : Molecular-Cloning
361. cloning: band at different site than desired after RE digestion - (reply: 11)
362. GST TAG - (reply: 3)
363. Residues on the N terminal GST Tag - (reply: 1)
364. Where to place the polyA signal? - (reply: 1)
365. identification of target sequence - (reply: 3)
366. cloning pcr insert into plasmid - (reply: 2)
367. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
368. Large quantity heat shock trouble? - (reply: 3)
369. molecular cloning - (reply: 7)
370. Cannot see insert with colony pcr - (reply: 6)
371. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
372. help with a vector - (reply: 3)
373. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
374. Another cloning hell....no transformants - (reply: 1)
375. Agrobacterium LBA4404 transformation - (reply: 1)
376. TOPO TA Cloning Troubleshooting - (reply: 1)
377. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
378. Tricistronic vector - (reply: 1)
379. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
380. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
381. Directions of genes expression illustrations - (reply: 5)
382. Plasmid DNA at RT for a few days - (reply: 4)
383. TOPO TA Cloning - (reply: 1)
384. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
385. No insert in subcloning - (reply: 3)
386. Digest does not confirm presence of fragment - (reply: 5)
387. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
388. pGL3 Promoter replacing TSS bases - (reply: 1)
389. lipofectamine 2000:DNA complex stability - (reply: 2)
390. Problems with Ecoli glycerol stock - (reply: 6)