Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1261. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1262. Storage of gel extracted DNA before ligation - (reply: 3)
1263. Dual Luciferase Assay - (reply: 3)
1264. Ligation Problems - (reply: 2)
1265. NICKED DNA - (reply: 2)
1266. cDNA clone - (reply: 1)
1267. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1268. Chosing the right competent cells - (reply: 1)
1269. Checking PCR insert (into pGEMT vector) - (reply: 2)
1270. problem with pGEMT - (reply: 3)
1271. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1272. Luciferase GL4 siRNA - (reply: 5)
1273. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1274. Culture Grow well but No Plasmid - (reply: 2)
1275. Insertion Mutation in Insert Sequence - (reply: 2)
1276. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1277. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1278. Protein expression with T7 promoter - (reply: 1)
1279. Triple ligation not working - (reply: 1)
1280. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1281. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1282. Problem with Induction (inclusion bodies?) - (reply: 4)
1283. Colony PCR screen positive - insert digestion negative - (reply: 2)
1284. plasmid purification kit for transfection - (reply: 2)
1285. Which pGL4 vector to choose - (reply: 2)
1286. Qiagen midi prepare low yield - (reply: 6)
1287. Help: incompatible RE site subcloning - (reply: 1)
1288. need help cloning cdna of a known protein - (reply: 1)
1289. restriction problem - (reply: 3)
1290. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)