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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1262. Bacterial growth in agar plate with ampicillin - (reply: 4)
1263. problem in ligation and cloning - (reply: 1)
1264. Electrophoresis gel boxes and geldoc system - (reply: 2)
1265. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1266. problem with 293T cell co-transfection - (reply: 5)
1267. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1268. how CIP treatment is done - (reply: 3)
1269. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1270. Storage of gel extracted DNA before ligation - (reply: 3)
1271. Dual Luciferase Assay - (reply: 3)
1272. Ligation Problems - (reply: 2)
1273. NICKED DNA - (reply: 2)
1274. cDNA clone - (reply: 1)
1275. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1276. Chosing the right competent cells - (reply: 1)
1277. Checking PCR insert (into pGEMT vector) - (reply: 2)
1278. problem with pGEMT - (reply: 3)
1279. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1280. Luciferase GL4 siRNA - (reply: 5)
1281. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1282. Culture Grow well but No Plasmid - (reply: 2)
1283. Insertion Mutation in Insert Sequence - (reply: 2)
1284. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1285. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1286. Protein expression with T7 promoter - (reply: 1)
1287. Triple ligation not working - (reply: 1)
1288. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1289. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1290. Problem with Induction (inclusion bodies?) - (reply: 4)