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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. Maximum recovery time after electroporation - (reply: 2)
1262. pCLBABE puro retriviral vector - (reply: 1)
1263. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1264. Blunt-end cloning problem - (reply: 6)
1265. Supercoiling blocking restriction site? - (reply: 6)
1266. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1267. problem in gene cloning - (reply: 4)
1268. Odd point mutations - (reply: 23)
1269. Plasmid linearization by PCR - (reply: 4)
1270. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1271. can't make the cloning work - (reply: 3)
1272. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1273. cloning help - (reply: 9)
1274. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1275. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1276. Ligation trouble continues - subcloning ligation failure (reply: 9)
1277. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1278. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1279. colony PCR - (reply: 8)
1280. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1281. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1282. Truncated sequence - (reply: 1)
1283. gateway cloning - (reply: 3)
1284. Purifying and cloning 69nt product - (reply: 5)
1285. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1286. ligatin issues - (reply: 1)
1287. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1288. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1289. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1290. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)