Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1261. problem in ligation and cloning - (reply: 1)
1262. Electrophoresis gel boxes and geldoc system - (reply: 2)
1263. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1264. problem with 293T cell co-transfection - (reply: 5)
1265. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1266. how CIP treatment is done - (reply: 3)
1267. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1268. Storage of gel extracted DNA before ligation - (reply: 3)
1269. Dual Luciferase Assay - (reply: 3)
1270. Ligation Problems - (reply: 2)
1271. NICKED DNA - (reply: 2)
1272. cDNA clone - (reply: 1)
1273. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1274. Chosing the right competent cells - (reply: 1)
1275. Checking PCR insert (into pGEMT vector) - (reply: 2)
1276. problem with pGEMT - (reply: 3)
1277. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1278. Luciferase GL4 siRNA - (reply: 5)
1279. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1280. Culture Grow well but No Plasmid - (reply: 2)
1281. Insertion Mutation in Insert Sequence - (reply: 2)
1282. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1283. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1284. Protein expression with T7 promoter - (reply: 1)
1285. Triple ligation not working - (reply: 1)
1286. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1287. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1288. Problem with Induction (inclusion bodies?) - (reply: 4)
1289. Colony PCR screen positive - insert digestion negative - (reply: 2)
1290. plasmid purification kit for transfection - (reply: 2)