Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1261. Subcloning into pMIR-REPORT-luc - (reply: 7)
1262. Restriction digestion troubleshooting - (reply: 10)
1263. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1264. Which digestion enzyme? - (reply: 1)
1265. Cloning big fragment into smaller vector - (reply: 3)
1266. from where can i get the P1 phage - (reply: 1)
1267. multi way ligation or long range pcr? - (reply: 1)
1268. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1269. list of sequence to be added before RE site - (reply: 3)
1270. plasmid, BAC, or cosmid? - (reply: 3)
1271. Wierd trouble - dunno where my insert is?? (reply: 3)
1272. Large Molecular weight DNA Extarction - (reply: 7)
1273. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1274. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1275. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1276. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1277. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1278. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1279. Vector sequence included in (PCR) amplified insert - (reply: 2)
1280. COMPETENT CELL PREPARATION - (reply: 6)
1281. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1282. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1283. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1284. T A cloning problems - (reply: 3)
1285. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1286. Maximum recovery time after electroporation - (reply: 2)
1287. pCLBABE puro retriviral vector - (reply: 1)
1288. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1289. Blunt-end cloning problem - (reply: 6)
1290. Supercoiling blocking restriction site? - (reply: 6)