Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1261. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1262. how CIP treatment is done - (reply: 3)
1263. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1264. Storage of gel extracted DNA before ligation - (reply: 3)
1265. Dual Luciferase Assay - (reply: 3)
1266. Ligation Problems - (reply: 2)
1267. NICKED DNA - (reply: 2)
1268. cDNA clone - (reply: 1)
1269. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1270. Chosing the right competent cells - (reply: 1)
1271. Checking PCR insert (into pGEMT vector) - (reply: 2)
1272. problem with pGEMT - (reply: 3)
1273. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1274. Luciferase GL4 siRNA - (reply: 5)
1275. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1276. Culture Grow well but No Plasmid - (reply: 2)
1277. Insertion Mutation in Insert Sequence - (reply: 2)
1278. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1279. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1280. Protein expression with T7 promoter - (reply: 1)
1281. Triple ligation not working - (reply: 1)
1282. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1283. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1284. Problem with Induction (inclusion bodies?) - (reply: 4)
1285. Colony PCR screen positive - insert digestion negative - (reply: 2)
1286. plasmid purification kit for transfection - (reply: 2)
1287. Which pGL4 vector to choose - (reply: 2)
1288. Qiagen midi prepare low yield - (reply: 6)
1289. Help: incompatible RE site subcloning - (reply: 1)
1290. need help cloning cdna of a known protein - (reply: 1)