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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. how to express the gene in opposite transcriptional direction - (reply: 3)
1262. Water for electrocompetent cells - (reply: 2)
1263. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1264. Bacterial growth in agar plate with ampicillin - (reply: 4)
1265. problem in ligation and cloning - (reply: 1)
1266. Electrophoresis gel boxes and geldoc system - (reply: 2)
1267. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1268. problem with 293T cell co-transfection - (reply: 5)
1269. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1270. how CIP treatment is done - (reply: 3)
1271. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1272. Storage of gel extracted DNA before ligation - (reply: 3)
1273. Dual Luciferase Assay - (reply: 3)
1274. Ligation Problems - (reply: 2)
1275. NICKED DNA - (reply: 2)
1276. cDNA clone - (reply: 1)
1277. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1278. Chosing the right competent cells - (reply: 1)
1279. Checking PCR insert (into pGEMT vector) - (reply: 2)
1280. problem with pGEMT - (reply: 3)
1281. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1282. Luciferase GL4 siRNA - (reply: 5)
1283. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1284. Culture Grow well but No Plasmid - (reply: 2)
1285. Insertion Mutation in Insert Sequence - (reply: 2)
1286. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1287. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1288. Protein expression with T7 promoter - (reply: 1)
1289. Triple ligation not working - (reply: 1)
1290. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)