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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. Bacterial growth in agar plate with ampicillin - (reply: 4)
1262. problem in ligation and cloning - (reply: 1)
1263. Electrophoresis gel boxes and geldoc system - (reply: 2)
1264. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1265. problem with 293T cell co-transfection - (reply: 5)
1266. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1267. how CIP treatment is done - (reply: 3)
1268. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1269. Storage of gel extracted DNA before ligation - (reply: 3)
1270. Dual Luciferase Assay - (reply: 3)
1271. Ligation Problems - (reply: 2)
1272. NICKED DNA - (reply: 2)
1273. cDNA clone - (reply: 1)
1274. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1275. Chosing the right competent cells - (reply: 1)
1276. Checking PCR insert (into pGEMT vector) - (reply: 2)
1277. problem with pGEMT - (reply: 3)
1278. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1279. Luciferase GL4 siRNA - (reply: 5)
1280. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1281. Culture Grow well but No Plasmid - (reply: 2)
1282. Insertion Mutation in Insert Sequence - (reply: 2)
1283. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1284. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1285. Protein expression with T7 promoter - (reply: 1)
1286. Triple ligation not working - (reply: 1)
1287. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1288. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1289. Problem with Induction (inclusion bodies?) - (reply: 4)
1290. Colony PCR screen positive - insert digestion negative - (reply: 2)