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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1262. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1263. Trouble with subcloning - (reply: 6)
1264. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1265. Lb-kan plates life - How long can they be used? (reply: 2)
1266. LR reaction - Cloning into a expression vector (reply: 1)
1267. TOPO-Transformation Problem - (reply: 4)
1268. Checking Plasmid Expression - (reply: 1)
1269. designing primers for genes not sequenced yet - (reply: 2)
1270. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1271. Cloning Problem - (reply: 3)
1272. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1273. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1274. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1275. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1276. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1277. Restriction buffer composition - (reply: 2)
1278. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1279. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1280. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1281. Yet another ligation problem - (reply: 4)
1282. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1283. Promoter Cloning for Luciferase Assay - (reply: 3)
1284. Creating mutants - (reply: 3)
1285. cloning protocol - (reply: 1)
1286. Ligation Time and Temp - (reply: 4)
1287. Miniprep - (reply: 3)
1288. PCR and then ligation - (reply: 7)
1289. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1290. 3 way ligation help needed - (reply: 10)