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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. 5' RACE for very GC-rich gene - (reply: 2)
1262. Phosphorylated primers - (reply: 5)
1263. Correct orientation of the insert - (reply: 7)
1264. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1265. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1266. Cloning advice needed - (reply: 4)
1267. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1268. Colony Screeing without using PCR - (reply: 8)
1269. blue white screening - (reply: 3)
1270. phosphorylate dprimers for pfx do not work - (reply: 3)
1271. help - (reply: 1)
1272. problem with yeast transformation - (reply: 2)
1273. ta cloning of pfu amplified fragment - (reply: 9)
1274. how to express the gene in opposite transcriptional direction - (reply: 3)
1275. Water for electrocompetent cells - (reply: 2)
1276. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1277. Bacterial growth in agar plate with ampicillin - (reply: 4)
1278. problem in ligation and cloning - (reply: 1)
1279. Electrophoresis gel boxes and geldoc system - (reply: 2)
1280. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1281. problem with 293T cell co-transfection - (reply: 5)
1282. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1283. how CIP treatment is done - (reply: 3)
1284. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1285. Storage of gel extracted DNA before ligation - (reply: 3)
1286. Dual Luciferase Assay - (reply: 3)
1287. Ligation Problems - (reply: 2)
1288. NICKED DNA - (reply: 2)
1289. cDNA clone - (reply: 1)
1290. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)