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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. multi way ligation or long range pcr? - (reply: 1)
1262. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1263. list of sequence to be added before RE site - (reply: 3)
1264. plasmid, BAC, or cosmid? - (reply: 3)
1265. Wierd trouble - dunno where my insert is?? (reply: 3)
1266. Large Molecular weight DNA Extarction - (reply: 7)
1267. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1268. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1269. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1270. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1271. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1272. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1273. Vector sequence included in (PCR) amplified insert - (reply: 2)
1274. COMPETENT CELL PREPARATION - (reply: 6)
1275. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1276. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1277. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1278. T A cloning problems - (reply: 3)
1279. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1280. Maximum recovery time after electroporation - (reply: 2)
1281. pCLBABE puro retriviral vector - (reply: 1)
1282. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1283. Blunt-end cloning problem - (reply: 6)
1284. Supercoiling blocking restriction site? - (reply: 6)
1285. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1286. problem in gene cloning - (reply: 4)
1287. Odd point mutations - (reply: 23)
1288. Plasmid linearization by PCR - (reply: 4)
1289. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1290. can't make the cloning work - (reply: 3)