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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1262. problem with 293T cell co-transfection - (reply: 5)
1263. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1264. how CIP treatment is done - (reply: 3)
1265. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1266. Storage of gel extracted DNA before ligation - (reply: 3)
1267. Dual Luciferase Assay - (reply: 3)
1268. Ligation Problems - (reply: 2)
1269. NICKED DNA - (reply: 2)
1270. cDNA clone - (reply: 1)
1271. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1272. Chosing the right competent cells - (reply: 1)
1273. Checking PCR insert (into pGEMT vector) - (reply: 2)
1274. problem with pGEMT - (reply: 3)
1275. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1276. Luciferase GL4 siRNA - (reply: 5)
1277. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1278. Culture Grow well but No Plasmid - (reply: 2)
1279. Insertion Mutation in Insert Sequence - (reply: 2)
1280. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1281. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1282. Protein expression with T7 promoter - (reply: 1)
1283. Triple ligation not working - (reply: 1)
1284. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1285. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1286. Problem with Induction (inclusion bodies?) - (reply: 4)
1287. Colony PCR screen positive - insert digestion negative - (reply: 2)
1288. plasmid purification kit for transfection - (reply: 2)
1289. Which pGL4 vector to choose - (reply: 2)
1290. Qiagen midi prepare low yield - (reply: 6)