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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1262. Cloning advice needed - (reply: 4)
1263. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1264. Colony Screeing without using PCR - (reply: 8)
1265. blue white screening - (reply: 3)
1266. phosphorylate dprimers for pfx do not work - (reply: 3)
1267. help - (reply: 1)
1268. problem with yeast transformation - (reply: 2)
1269. ta cloning of pfu amplified fragment - (reply: 9)
1270. how to express the gene in opposite transcriptional direction - (reply: 3)
1271. Water for electrocompetent cells - (reply: 2)
1272. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1273. Bacterial growth in agar plate with ampicillin - (reply: 4)
1274. problem in ligation and cloning - (reply: 1)
1275. Electrophoresis gel boxes and geldoc system - (reply: 2)
1276. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1277. problem with 293T cell co-transfection - (reply: 5)
1278. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1279. how CIP treatment is done - (reply: 3)
1280. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1281. Storage of gel extracted DNA before ligation - (reply: 3)
1282. Dual Luciferase Assay - (reply: 3)
1283. Ligation Problems - (reply: 2)
1284. NICKED DNA - (reply: 2)
1285. cDNA clone - (reply: 1)
1286. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1287. Chosing the right competent cells - (reply: 1)
1288. Checking PCR insert (into pGEMT vector) - (reply: 2)
1289. problem with pGEMT - (reply: 3)
1290. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)