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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1262. Promoter Cloning for Luciferase Assay - (reply: 3)
1263. Creating mutants - (reply: 3)
1264. cloning protocol - (reply: 1)
1265. Ligation Time and Temp - (reply: 4)
1266. Miniprep - (reply: 3)
1267. PCR and then ligation - (reply: 7)
1268. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1269. 3 way ligation help needed - (reply: 10)
1270. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1271. Cloning woes - Running out of ideas! - (reply: 7)
1272. What fields of research other than microbiology work with bacteria and - (reply: 1)
1273. How to inoculate bacteria? - (reply: 10)
1274. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1275. need help in my Fse1 restriction ezyme - (reply: 4)
1276. Insert problems with TA cloning - (reply: 1)
1277. Surface expression by tansfection of CHO - (reply: 3)
1278. please help!!! with transformation - (reply: 10)
1279. sequencing primer: T7 or T7promoter? - (reply: 4)
1280. Unstable clone? - (reply: 1)
1281. Restriction enzymes - (reply: 3)
1282. Problems facing in cloning with pBI121 vector - (reply: 1)
1283. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1284. Is my insert present??? - (reply: 17)
1285. Need help for restriction enzymes - (reply: 5)
1286. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1287. Mini prep - (reply: 4)
1288. adding koazak sequence to primer - (reply: 1)
1289. DNA ligation - (reply: 2)
1290. adding C terminal tag to reverse primer - (reply: 1)