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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1262. Luciferase GL4 siRNA - (reply: 5)
1263. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1264. Culture Grow well but No Plasmid - (reply: 2)
1265. Insertion Mutation in Insert Sequence - (reply: 2)
1266. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1267. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1268. Protein expression with T7 promoter - (reply: 1)
1269. Triple ligation not working - (reply: 1)
1270. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1271. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1272. Problem with Induction (inclusion bodies?) - (reply: 4)
1273. Colony PCR screen positive - insert digestion negative - (reply: 2)
1274. plasmid purification kit for transfection - (reply: 2)
1275. Which pGL4 vector to choose - (reply: 2)
1276. Qiagen midi prepare low yield - (reply: 6)
1277. Help: incompatible RE site subcloning - (reply: 1)
1278. need help cloning cdna of a known protein - (reply: 1)
1279. restriction problem - (reply: 3)
1280. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1281. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1282. Trouble with subcloning - (reply: 6)
1283. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1284. Lb-kan plates life - How long can they be used? (reply: 2)
1285. LR reaction - Cloning into a expression vector (reply: 1)
1286. TOPO-Transformation Problem - (reply: 4)
1287. Checking Plasmid Expression - (reply: 1)
1288. designing primers for genes not sequenced yet - (reply: 2)
1289. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1290. Cloning Problem - (reply: 3)