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Top : New Forum Archives (2009-): : Molecular-Cloning
1261. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1262. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1263. Protein expression with T7 promoter - (reply: 1)
1264. Triple ligation not working - (reply: 1)
1265. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1266. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1267. Problem with Induction (inclusion bodies?) - (reply: 4)
1268. Colony PCR screen positive - insert digestion negative - (reply: 2)
1269. plasmid purification kit for transfection - (reply: 2)
1270. Which pGL4 vector to choose - (reply: 2)
1271. Qiagen midi prepare low yield - (reply: 6)
1272. Help: incompatible RE site subcloning - (reply: 1)
1273. need help cloning cdna of a known protein - (reply: 1)
1274. restriction problem - (reply: 3)
1275. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1276. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1277. Trouble with subcloning - (reply: 6)
1278. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1279. Lb-kan plates life - How long can they be used? (reply: 2)
1280. LR reaction - Cloning into a expression vector (reply: 1)
1281. TOPO-Transformation Problem - (reply: 4)
1282. Checking Plasmid Expression - (reply: 1)
1283. designing primers for genes not sequenced yet - (reply: 2)
1284. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1285. Cloning Problem - (reply: 3)
1286. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1287. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1288. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1289. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1290. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)