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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1232. Storage of gel extracted DNA before ligation - (reply: 3)
1233. Dual Luciferase Assay - (reply: 3)
1234. Ligation Problems - (reply: 2)
1235. NICKED DNA - (reply: 2)
1236. cDNA clone - (reply: 1)
1237. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1238. Chosing the right competent cells - (reply: 1)
1239. Checking PCR insert (into pGEMT vector) - (reply: 2)
1240. problem with pGEMT - (reply: 3)
1241. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1242. Luciferase GL4 siRNA - (reply: 5)
1243. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1244. Culture Grow well but No Plasmid - (reply: 2)
1245. Insertion Mutation in Insert Sequence - (reply: 2)
1246. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1247. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1248. Protein expression with T7 promoter - (reply: 1)
1249. Triple ligation not working - (reply: 1)
1250. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1251. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1252. Problem with Induction (inclusion bodies?) - (reply: 4)
1253. Colony PCR screen positive - insert digestion negative - (reply: 2)
1254. plasmid purification kit for transfection - (reply: 2)
1255. Which pGL4 vector to choose - (reply: 2)
1256. Qiagen midi prepare low yield - (reply: 6)
1257. Help: incompatible RE site subcloning - (reply: 1)
1258. need help cloning cdna of a known protein - (reply: 1)
1259. restriction problem - (reply: 3)
1260. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)