Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Neon Transfection System - (reply: 2)
1232. help - (reply: 5)
1233. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1234. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1235. problems with qiagen gel extraction kit - (reply: 7)
1236. restriction enzyme flanking sequence preferance - (reply: 2)
1237. pCDH Lentivector has 500 bp insert - (reply: 5)
1238. 5' RACE for very GC-rich gene - (reply: 2)
1239. Phosphorylated primers - (reply: 5)
1240. Correct orientation of the insert - (reply: 7)
1241. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1242. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1243. Cloning advice needed - (reply: 4)
1244. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1245. Colony Screeing without using PCR - (reply: 8)
1246. blue white screening - (reply: 3)
1247. phosphorylate dprimers for pfx do not work - (reply: 3)
1248. help - (reply: 1)
1249. problem with yeast transformation - (reply: 2)
1250. ta cloning of pfu amplified fragment - (reply: 9)
1251. how to express the gene in opposite transcriptional direction - (reply: 3)
1252. Water for electrocompetent cells - (reply: 2)
1253. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1254. Bacterial growth in agar plate with ampicillin - (reply: 4)
1255. problem in ligation and cloning - (reply: 1)
1256. Electrophoresis gel boxes and geldoc system - (reply: 2)
1257. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1258. problem with 293T cell co-transfection - (reply: 5)
1259. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1260. how CIP treatment is done - (reply: 3)