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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Restriction buffer composition - (reply: 2)
1232. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1233. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1234. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1235. Yet another ligation problem - (reply: 4)
1236. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1237. Promoter Cloning for Luciferase Assay - (reply: 3)
1238. Creating mutants - (reply: 3)
1239. cloning protocol - (reply: 1)
1240. Ligation Time and Temp - (reply: 4)
1241. Miniprep - (reply: 3)
1242. PCR and then ligation - (reply: 7)
1243. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1244. 3 way ligation help needed - (reply: 10)
1245. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1246. Cloning woes - Running out of ideas! - (reply: 7)
1247. What fields of research other than microbiology work with bacteria and - (reply: 1)
1248. How to inoculate bacteria? - (reply: 10)
1249. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1250. need help in my Fse1 restriction ezyme - (reply: 4)
1251. Insert problems with TA cloning - (reply: 1)
1252. Surface expression by tansfection of CHO - (reply: 3)
1253. please help!!! with transformation - (reply: 10)
1254. sequencing primer: T7 or T7promoter? - (reply: 4)
1255. Unstable clone? - (reply: 1)
1256. Restriction enzymes - (reply: 3)
1257. Problems facing in cloning with pBI121 vector - (reply: 1)
1258. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1259. Is my insert present??? - (reply: 17)
1260. Need help for restriction enzymes - (reply: 5)