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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. re-digestion - (reply: 1)
1232. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1233. Cloning using BamH1 and HindIII - (reply: 8)
1234. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1235. "competentcy" of my method - (reply: 5)
1236. Vector frameshift problem - (reply: 3)
1237. Screening colonies after transformation - (reply: 2)
1238. z competent cells - (reply: 2)
1239. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1240. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1241. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1242. plasmid extraction kit wash buffer solution - (reply: 3)
1243. molecular "terminology" - (reply: 1)
1244. NdeI digestion - Share your experience (reply: 3)
1245. Multiple bands after amping insert - (reply: 2)
1246. Good tool for sequence validation? - For Windows (reply: 2)
1247. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1248. Silencing of the first cistron - (reply: 1)
1249. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1250. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1251. Stable Mammalian Expression with Retrovirus - (reply: 2)
1252. Neon Transfection System - (reply: 2)
1253. help - (reply: 5)
1254. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1255. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1256. problems with qiagen gel extraction kit - (reply: 7)
1257. restriction enzyme flanking sequence preferance - (reply: 2)
1258. pCDH Lentivector has 500 bp insert - (reply: 5)
1259. 5' RACE for very GC-rich gene - (reply: 2)
1260. Phosphorylated primers - (reply: 5)