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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Subcloning into pMIR-REPORT-luc - (reply: 7)
1232. Restriction digestion troubleshooting - (reply: 10)
1233. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1234. Which digestion enzyme? - (reply: 1)
1235. Cloning big fragment into smaller vector - (reply: 3)
1236. from where can i get the P1 phage - (reply: 1)
1237. multi way ligation or long range pcr? - (reply: 1)
1238. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1239. list of sequence to be added before RE site - (reply: 3)
1240. plasmid, BAC, or cosmid? - (reply: 3)
1241. Wierd trouble - dunno where my insert is?? (reply: 3)
1242. Large Molecular weight DNA Extarction - (reply: 7)
1243. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1244. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1245. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1246. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1247. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1248. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1249. Vector sequence included in (PCR) amplified insert - (reply: 2)
1250. COMPETENT CELL PREPARATION - (reply: 6)
1251. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1252. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1253. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1254. T A cloning problems - (reply: 3)
1255. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1256. Maximum recovery time after electroporation - (reply: 2)
1257. pCLBABE puro retriviral vector - (reply: 1)
1258. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1259. Blunt-end cloning problem - (reply: 6)
1260. Supercoiling blocking restriction site? - (reply: 6)