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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. NdeI digestion - Share your experience (reply: 3)
1232. Multiple bands after amping insert - (reply: 2)
1233. Good tool for sequence validation? - For Windows (reply: 2)
1234. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1235. Silencing of the first cistron - (reply: 1)
1236. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1237. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1238. Stable Mammalian Expression with Retrovirus - (reply: 2)
1239. Neon Transfection System - (reply: 2)
1240. help - (reply: 5)
1241. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1242. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1243. problems with qiagen gel extraction kit - (reply: 7)
1244. restriction enzyme flanking sequence preferance - (reply: 2)
1245. pCDH Lentivector has 500 bp insert - (reply: 5)
1246. 5' RACE for very GC-rich gene - (reply: 2)
1247. Phosphorylated primers - (reply: 5)
1248. Correct orientation of the insert - (reply: 7)
1249. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1250. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1251. Cloning advice needed - (reply: 4)
1252. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1253. Colony Screeing without using PCR - (reply: 8)
1254. blue white screening - (reply: 3)
1255. phosphorylate dprimers for pfx do not work - (reply: 3)
1256. help - (reply: 1)
1257. problem with yeast transformation - (reply: 2)
1258. ta cloning of pfu amplified fragment - (reply: 9)
1259. how to express the gene in opposite transcriptional direction - (reply: 3)
1260. Water for electrocompetent cells - (reply: 2)