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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Good tool for sequence validation? - For Windows (reply: 2)
1232. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1233. Silencing of the first cistron - (reply: 1)
1234. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1235. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1236. Stable Mammalian Expression with Retrovirus - (reply: 2)
1237. Neon Transfection System - (reply: 2)
1238. help - (reply: 5)
1239. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1240. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1241. problems with qiagen gel extraction kit - (reply: 7)
1242. restriction enzyme flanking sequence preferance - (reply: 2)
1243. pCDH Lentivector has 500 bp insert - (reply: 5)
1244. 5' RACE for very GC-rich gene - (reply: 2)
1245. Phosphorylated primers - (reply: 5)
1246. Correct orientation of the insert - (reply: 7)
1247. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1248. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1249. Cloning advice needed - (reply: 4)
1250. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1251. Colony Screeing without using PCR - (reply: 8)
1252. blue white screening - (reply: 3)
1253. phosphorylate dprimers for pfx do not work - (reply: 3)
1254. help - (reply: 1)
1255. problem with yeast transformation - (reply: 2)
1256. ta cloning of pfu amplified fragment - (reply: 9)
1257. how to express the gene in opposite transcriptional direction - (reply: 3)
1258. Water for electrocompetent cells - (reply: 2)
1259. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1260. Bacterial growth in agar plate with ampicillin - (reply: 4)