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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. My battle with BamHI - (reply: 35)
1232. serious problem in cloning - (reply: 2)
1233. What are "Ella-cre" mice? - (reply: 2)
1234. help with pCMV-myc vector - (reply: 2)
1235. Elution amount - (reply: 5)
1236. keeping restriction digested fragments at -20? - (reply: 5)
1237. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1238. re-digestion - (reply: 1)
1239. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1240. Cloning using BamH1 and HindIII - (reply: 8)
1241. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1242. "competentcy" of my method - (reply: 5)
1243. Vector frameshift problem - (reply: 3)
1244. Screening colonies after transformation - (reply: 2)
1245. z competent cells - (reply: 2)
1246. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1247. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1248. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1249. plasmid extraction kit wash buffer solution - (reply: 3)
1250. molecular "terminology" - (reply: 1)
1251. NdeI digestion - Share your experience (reply: 3)
1252. Multiple bands after amping insert - (reply: 2)
1253. Good tool for sequence validation? - For Windows (reply: 2)
1254. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1255. Silencing of the first cistron - (reply: 1)
1256. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1257. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1258. Stable Mammalian Expression with Retrovirus - (reply: 2)
1259. Neon Transfection System - (reply: 2)
1260. help - (reply: 5)