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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Silencing of the first cistron - (reply: 1)
1232. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1233. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1234. Stable Mammalian Expression with Retrovirus - (reply: 2)
1235. Neon Transfection System - (reply: 2)
1236. help - (reply: 5)
1237. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1238. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1239. problems with qiagen gel extraction kit - (reply: 7)
1240. restriction enzyme flanking sequence preferance - (reply: 2)
1241. pCDH Lentivector has 500 bp insert - (reply: 5)
1242. 5' RACE for very GC-rich gene - (reply: 2)
1243. Phosphorylated primers - (reply: 5)
1244. Correct orientation of the insert - (reply: 7)
1245. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1246. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1247. Cloning advice needed - (reply: 4)
1248. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1249. Colony Screeing without using PCR - (reply: 8)
1250. blue white screening - (reply: 3)
1251. phosphorylate dprimers for pfx do not work - (reply: 3)
1252. help - (reply: 1)
1253. problem with yeast transformation - (reply: 2)
1254. ta cloning of pfu amplified fragment - (reply: 9)
1255. how to express the gene in opposite transcriptional direction - (reply: 3)
1256. Water for electrocompetent cells - (reply: 2)
1257. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1258. Bacterial growth in agar plate with ampicillin - (reply: 4)
1259. problem in ligation and cloning - (reply: 1)
1260. Electrophoresis gel boxes and geldoc system - (reply: 2)