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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1232. re-digestion - (reply: 1)
1233. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1234. Cloning using BamH1 and HindIII - (reply: 8)
1235. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1236. "competentcy" of my method - (reply: 5)
1237. Vector frameshift problem - (reply: 3)
1238. Screening colonies after transformation - (reply: 2)
1239. z competent cells - (reply: 2)
1240. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1241. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1242. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1243. plasmid extraction kit wash buffer solution - (reply: 3)
1244. molecular "terminology" - (reply: 1)
1245. NdeI digestion - Share your experience (reply: 3)
1246. Multiple bands after amping insert - (reply: 2)
1247. Good tool for sequence validation? - For Windows (reply: 2)
1248. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1249. Silencing of the first cistron - (reply: 1)
1250. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1251. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1252. Stable Mammalian Expression with Retrovirus - (reply: 2)
1253. Neon Transfection System - (reply: 2)
1254. help - (reply: 5)
1255. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1256. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1257. problems with qiagen gel extraction kit - (reply: 7)
1258. restriction enzyme flanking sequence preferance - (reply: 2)
1259. pCDH Lentivector has 500 bp insert - (reply: 5)
1260. 5' RACE for very GC-rich gene - (reply: 2)