Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Cloning using BamH1 and HindIII - (reply: 8)
1232. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1233. "competentcy" of my method - (reply: 5)
1234. Vector frameshift problem - (reply: 3)
1235. Screening colonies after transformation - (reply: 2)
1236. z competent cells - (reply: 2)
1237. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1238. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1239. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1240. plasmid extraction kit wash buffer solution - (reply: 3)
1241. molecular "terminology" - (reply: 1)
1242. NdeI digestion - Share your experience (reply: 3)
1243. Multiple bands after amping insert - (reply: 2)
1244. Good tool for sequence validation? - For Windows (reply: 2)
1245. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1246. Silencing of the first cistron - (reply: 1)
1247. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1248. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1249. Stable Mammalian Expression with Retrovirus - (reply: 2)
1250. Neon Transfection System - (reply: 2)
1251. help - (reply: 5)
1252. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1253. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1254. problems with qiagen gel extraction kit - (reply: 7)
1255. restriction enzyme flanking sequence preferance - (reply: 2)
1256. pCDH Lentivector has 500 bp insert - (reply: 5)
1257. 5' RACE for very GC-rich gene - (reply: 2)
1258. Phosphorylated primers - (reply: 5)
1259. Correct orientation of the insert - (reply: 7)
1260. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)