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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. need help in my Fse1 restriction ezyme - (reply: 4)
1232. Insert problems with TA cloning - (reply: 1)
1233. Surface expression by tansfection of CHO - (reply: 3)
1234. please help!!! with transformation - (reply: 10)
1235. sequencing primer: T7 or T7promoter? - (reply: 4)
1236. Unstable clone? - (reply: 1)
1237. Restriction enzymes - (reply: 3)
1238. Problems facing in cloning with pBI121 vector - (reply: 1)
1239. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1240. Is my insert present??? - (reply: 17)
1241. Need help for restriction enzymes - (reply: 5)
1242. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1243. Mini prep - (reply: 4)
1244. adding koazak sequence to primer - (reply: 1)
1245. DNA ligation - (reply: 2)
1246. adding C terminal tag to reverse primer - (reply: 1)
1247. glycerol stocks - (reply: 10)
1248. doubts in sequence submission to NCBI - (reply: 2)
1249. Concentration of DNA for Transformation - (reply: 1)
1250. Restriction digest - (reply: 7)
1251. Help! Double restriction digestion failed.... - (reply: 6)
1252. Kozak sequences.. - (reply: 1)
1253. Sequence management software (for Mac) - (reply: 5)
1254. ligation for 24 hrs in 4*C? - (reply: 3)
1255. Having problem establishing stable cell line...... - (reply: 5)
1256. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)
1257. Contruction of Expression Construct - (reply: 1)
1258. problem with subcloning, please help! - (reply: 8)
1259. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
1260. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)