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1231. DH5a comp cells - (reply: 5)
1232. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1233. Monocot promoters - (reply: 1)
1234. ligation problem - (reply: 11)
1235. How Gateway cloning system works? - (reply: 1)
1236. Chemically competent to electrocompetent DH5alpha - (reply: 1)
1237. Tetracycline concnetration in selection - (reply: 1)
1238. pCS2 - (reply: 2)
1239. What does this look like to you? - (reply: 23)
1240. ds-oligo cloning/ligation issues - (reply: 18)
1241. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
1242. CIP required for double digestion of plasmid? - (reply: 3)
1243. Help with cloning a plasmid that contains repeats! - (reply: 2)
1244. To what extent competent cells be thawed before transformation? - (reply: 4)
1245. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1246. size of gene insert wrong? - (reply: 3)
1247. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1248. yeast transformation - how to make a thermotolerant yeast (reply: 4)
1249. need help with klenow - (reply: 4)
1250. Subcloning into pBluescript - (reply: 6)
1251. How to control cloned cDNA expression level in cells? - (reply: 8)
1252. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1253. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1254. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1255. Gel purification of vector DNA - low yield - (reply: 26)
1256. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1257. Identifying cDNA insert - Basic help needed (reply: 2)
1258. gel purification or ligation problem - (reply: 3)
1259. Sequential restriction digestion of plasmid - (reply: 58)
1260. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1232. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1233. Monocot promoters - (reply: 1)
1234. ligation problem - (reply: 11)
1235. How Gateway cloning system works? - (reply: 1)
1236. Chemically competent to electrocompetent DH5alpha - (reply: 1)
1237. Tetracycline concnetration in selection - (reply: 1)
1238. pCS2 - (reply: 2)
1239. What does this look like to you? - (reply: 23)
1240. ds-oligo cloning/ligation issues - (reply: 18)
1241. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
1242. CIP required for double digestion of plasmid? - (reply: 3)
1243. Help with cloning a plasmid that contains repeats! - (reply: 2)
1244. To what extent competent cells be thawed before transformation? - (reply: 4)
1245. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1246. size of gene insert wrong? - (reply: 3)
1247. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1248. yeast transformation - how to make a thermotolerant yeast (reply: 4)
1249. need help with klenow - (reply: 4)
1250. Subcloning into pBluescript - (reply: 6)
1251. How to control cloned cDNA expression level in cells? - (reply: 8)
1252. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1253. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1254. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1255. Gel purification of vector DNA - low yield - (reply: 26)
1256. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1257. Identifying cDNA insert - Basic help needed (reply: 2)
1258. gel purification or ligation problem - (reply: 3)
1259. Sequential restriction digestion of plasmid - (reply: 58)
1260. Site-directed mutagenesis - nucleotides insertion (reply: 1)