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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Dual Luciferase Assay - (reply: 3)
1232. Ligation Problems - (reply: 2)
1233. NICKED DNA - (reply: 2)
1234. cDNA clone - (reply: 1)
1235. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1236. Chosing the right competent cells - (reply: 1)
1237. Checking PCR insert (into pGEMT vector) - (reply: 2)
1238. problem with pGEMT - (reply: 3)
1239. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1240. Luciferase GL4 siRNA - (reply: 5)
1241. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1242. Culture Grow well but No Plasmid - (reply: 2)
1243. Insertion Mutation in Insert Sequence - (reply: 2)
1244. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1245. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1246. Protein expression with T7 promoter - (reply: 1)
1247. Triple ligation not working - (reply: 1)
1248. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1249. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1250. Problem with Induction (inclusion bodies?) - (reply: 4)
1251. Colony PCR screen positive - insert digestion negative - (reply: 2)
1252. plasmid purification kit for transfection - (reply: 2)
1253. Which pGL4 vector to choose - (reply: 2)
1254. Qiagen midi prepare low yield - (reply: 6)
1255. Help: incompatible RE site subcloning - (reply: 1)
1256. need help cloning cdna of a known protein - (reply: 1)
1257. restriction problem - (reply: 3)
1258. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1259. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1260. Trouble with subcloning - (reply: 6)