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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Elution amount - (reply: 5)
1232. keeping restriction digested fragments at -20? - (reply: 5)
1233. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1234. re-digestion - (reply: 1)
1235. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1236. Cloning using BamH1 and HindIII - (reply: 8)
1237. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1238. "competentcy" of my method - (reply: 5)
1239. Vector frameshift problem - (reply: 3)
1240. Screening colonies after transformation - (reply: 2)
1241. z competent cells - (reply: 2)
1242. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1243. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1244. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1245. plasmid extraction kit wash buffer solution - (reply: 3)
1246. molecular "terminology" - (reply: 1)
1247. NdeI digestion - Share your experience (reply: 3)
1248. Multiple bands after amping insert - (reply: 2)
1249. Good tool for sequence validation? - For Windows (reply: 2)
1250. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1251. Silencing of the first cistron - (reply: 1)
1252. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1253. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1254. Stable Mammalian Expression with Retrovirus - (reply: 2)
1255. Neon Transfection System - (reply: 2)
1256. help - (reply: 5)
1257. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1258. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1259. problems with qiagen gel extraction kit - (reply: 7)
1260. restriction enzyme flanking sequence preferance - (reply: 2)