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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. Stable Mammalian Expression with Retrovirus - (reply: 2)
1232. Neon Transfection System - (reply: 2)
1233. help - (reply: 5)
1234. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1235. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1236. problems with qiagen gel extraction kit - (reply: 7)
1237. restriction enzyme flanking sequence preferance - (reply: 2)
1238. pCDH Lentivector has 500 bp insert - (reply: 5)
1239. 5' RACE for very GC-rich gene - (reply: 2)
1240. Phosphorylated primers - (reply: 5)
1241. Correct orientation of the insert - (reply: 7)
1242. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1243. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1244. Cloning advice needed - (reply: 4)
1245. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1246. Colony Screeing without using PCR - (reply: 8)
1247. blue white screening - (reply: 3)
1248. phosphorylate dprimers for pfx do not work - (reply: 3)
1249. help - (reply: 1)
1250. problem with yeast transformation - (reply: 2)
1251. ta cloning of pfu amplified fragment - (reply: 9)
1252. how to express the gene in opposite transcriptional direction - (reply: 3)
1253. Water for electrocompetent cells - (reply: 2)
1254. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1255. Bacterial growth in agar plate with ampicillin - (reply: 4)
1256. problem in ligation and cloning - (reply: 1)
1257. Electrophoresis gel boxes and geldoc system - (reply: 2)
1258. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1259. problem with 293T cell co-transfection - (reply: 5)
1260. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)