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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1232. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1233. Cloning advice needed - (reply: 4)
1234. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1235. Colony Screeing without using PCR - (reply: 8)
1236. blue white screening - (reply: 3)
1237. phosphorylate dprimers for pfx do not work - (reply: 3)
1238. help - (reply: 1)
1239. problem with yeast transformation - (reply: 2)
1240. ta cloning of pfu amplified fragment - (reply: 9)
1241. how to express the gene in opposite transcriptional direction - (reply: 3)
1242. Water for electrocompetent cells - (reply: 2)
1243. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1244. Bacterial growth in agar plate with ampicillin - (reply: 4)
1245. problem in ligation and cloning - (reply: 1)
1246. Electrophoresis gel boxes and geldoc system - (reply: 2)
1247. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1248. problem with 293T cell co-transfection - (reply: 5)
1249. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1250. how CIP treatment is done - (reply: 3)
1251. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1252. Storage of gel extracted DNA before ligation - (reply: 3)
1253. Dual Luciferase Assay - (reply: 3)
1254. Ligation Problems - (reply: 2)
1255. NICKED DNA - (reply: 2)
1256. cDNA clone - (reply: 1)
1257. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1258. Chosing the right competent cells - (reply: 1)
1259. Checking PCR insert (into pGEMT vector) - (reply: 2)
1260. problem with pGEMT - (reply: 3)