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Top : New Forum Archives (2009-): : Molecular-Cloning
1231. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1232. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1233. problems with qiagen gel extraction kit - (reply: 7)
1234. restriction enzyme flanking sequence preferance - (reply: 2)
1235. pCDH Lentivector has 500 bp insert - (reply: 5)
1236. 5' RACE for very GC-rich gene - (reply: 2)
1237. Phosphorylated primers - (reply: 5)
1238. Correct orientation of the insert - (reply: 7)
1239. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1240. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1241. Cloning advice needed - (reply: 4)
1242. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1243. Colony Screeing without using PCR - (reply: 8)
1244. blue white screening - (reply: 3)
1245. phosphorylate dprimers for pfx do not work - (reply: 3)
1246. help - (reply: 1)
1247. problem with yeast transformation - (reply: 2)
1248. ta cloning of pfu amplified fragment - (reply: 9)
1249. how to express the gene in opposite transcriptional direction - (reply: 3)
1250. Water for electrocompetent cells - (reply: 2)
1251. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1252. Bacterial growth in agar plate with ampicillin - (reply: 4)
1253. problem in ligation and cloning - (reply: 1)
1254. Electrophoresis gel boxes and geldoc system - (reply: 2)
1255. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1256. problem with 293T cell co-transfection - (reply: 5)
1257. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1258. how CIP treatment is done - (reply: 3)
1259. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1260. Storage of gel extracted DNA before ligation - (reply: 3)