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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1202. Storage of gel extracted DNA before ligation - (reply: 3)
1203. Dual Luciferase Assay - (reply: 3)
1204. Ligation Problems - (reply: 2)
1205. NICKED DNA - (reply: 2)
1206. cDNA clone - (reply: 1)
1207. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1208. Chosing the right competent cells - (reply: 1)
1209. Checking PCR insert (into pGEMT vector) - (reply: 2)
1210. problem with pGEMT - (reply: 3)
1211. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1212. Luciferase GL4 siRNA - (reply: 5)
1213. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1214. Culture Grow well but No Plasmid - (reply: 2)
1215. Insertion Mutation in Insert Sequence - (reply: 2)
1216. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1217. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1218. Protein expression with T7 promoter - (reply: 1)
1219. Triple ligation not working - (reply: 1)
1220. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1221. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1222. Problem with Induction (inclusion bodies?) - (reply: 4)
1223. Colony PCR screen positive - insert digestion negative - (reply: 2)
1224. plasmid purification kit for transfection - (reply: 2)
1225. Which pGL4 vector to choose - (reply: 2)
1226. Qiagen midi prepare low yield - (reply: 6)
1227. Help: incompatible RE site subcloning - (reply: 1)
1228. need help cloning cdna of a known protein - (reply: 1)
1229. restriction problem - (reply: 3)
1230. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)