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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Cloning advice needed - (reply: 4)
1202. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1203. Colony Screeing without using PCR - (reply: 8)
1204. blue white screening - (reply: 3)
1205. phosphorylate dprimers for pfx do not work - (reply: 3)
1206. help - (reply: 1)
1207. problem with yeast transformation - (reply: 2)
1208. ta cloning of pfu amplified fragment - (reply: 9)
1209. how to express the gene in opposite transcriptional direction - (reply: 3)
1210. Water for electrocompetent cells - (reply: 2)
1211. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1212. Bacterial growth in agar plate with ampicillin - (reply: 4)
1213. problem in ligation and cloning - (reply: 1)
1214. Electrophoresis gel boxes and geldoc system - (reply: 2)
1215. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1216. problem with 293T cell co-transfection - (reply: 5)
1217. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1218. how CIP treatment is done - (reply: 3)
1219. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1220. Storage of gel extracted DNA before ligation - (reply: 3)
1221. Dual Luciferase Assay - (reply: 3)
1222. Ligation Problems - (reply: 2)
1223. NICKED DNA - (reply: 2)
1224. cDNA clone - (reply: 1)
1225. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1226. Chosing the right competent cells - (reply: 1)
1227. Checking PCR insert (into pGEMT vector) - (reply: 2)
1228. problem with pGEMT - (reply: 3)
1229. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1230. Luciferase GL4 siRNA - (reply: 5)