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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. blue white screening - (reply: 3)
1202. phosphorylate dprimers for pfx do not work - (reply: 3)
1203. help - (reply: 1)
1204. problem with yeast transformation - (reply: 2)
1205. ta cloning of pfu amplified fragment - (reply: 9)
1206. how to express the gene in opposite transcriptional direction - (reply: 3)
1207. Water for electrocompetent cells - (reply: 2)
1208. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1209. Bacterial growth in agar plate with ampicillin - (reply: 4)
1210. problem in ligation and cloning - (reply: 1)
1211. Electrophoresis gel boxes and geldoc system - (reply: 2)
1212. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1213. problem with 293T cell co-transfection - (reply: 5)
1214. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1215. how CIP treatment is done - (reply: 3)
1216. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1217. Storage of gel extracted DNA before ligation - (reply: 3)
1218. Dual Luciferase Assay - (reply: 3)
1219. Ligation Problems - (reply: 2)
1220. NICKED DNA - (reply: 2)
1221. cDNA clone - (reply: 1)
1222. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1223. Chosing the right competent cells - (reply: 1)
1224. Checking PCR insert (into pGEMT vector) - (reply: 2)
1225. problem with pGEMT - (reply: 3)
1226. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1227. Luciferase GL4 siRNA - (reply: 5)
1228. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1229. Culture Grow well but No Plasmid - (reply: 2)
1230. Insertion Mutation in Insert Sequence - (reply: 2)