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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1202. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1203. Nco1 digest problem - (reply: 4)
1204. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1205. ligation - (reply: 1)
1206. pre-prepararation of competent cells - (reply: 3)
1207. different copy # multimers with linker ligation? - (reply: 2)
1208. Help ADD 3 bases to DNA Sequences - (reply: 6)
1209. My battle with BamHI - (reply: 35)
1210. serious problem in cloning - (reply: 2)
1211. What are "Ella-cre" mice? - (reply: 2)
1212. help with pCMV-myc vector - (reply: 2)
1213. Elution amount - (reply: 5)
1214. keeping restriction digested fragments at -20? - (reply: 5)
1215. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1216. re-digestion - (reply: 1)
1217. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1218. Cloning using BamH1 and HindIII - (reply: 8)
1219. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1220. "competentcy" of my method - (reply: 5)
1221. Vector frameshift problem - (reply: 3)
1222. Screening colonies after transformation - (reply: 2)
1223. z competent cells - (reply: 2)
1224. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1225. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1226. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1227. plasmid extraction kit wash buffer solution - (reply: 3)
1228. molecular "terminology" - (reply: 1)
1229. NdeI digestion - Share your experience (reply: 3)
1230. Multiple bands after amping insert - (reply: 2)