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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1202. Cloning using BamH1 and HindIII - (reply: 8)
1203. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1204. "competentcy" of my method - (reply: 5)
1205. Vector frameshift problem - (reply: 3)
1206. Screening colonies after transformation - (reply: 2)
1207. z competent cells - (reply: 2)
1208. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1209. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1210. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1211. plasmid extraction kit wash buffer solution - (reply: 3)
1212. molecular "terminology" - (reply: 1)
1213. NdeI digestion - Share your experience (reply: 3)
1214. Multiple bands after amping insert - (reply: 2)
1215. Good tool for sequence validation? - For Windows (reply: 2)
1216. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1217. Silencing of the first cistron - (reply: 1)
1218. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1219. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1220. Stable Mammalian Expression with Retrovirus - (reply: 2)
1221. Neon Transfection System - (reply: 2)
1222. help - (reply: 5)
1223. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1224. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1225. problems with qiagen gel extraction kit - (reply: 7)
1226. restriction enzyme flanking sequence preferance - (reply: 2)
1227. pCDH Lentivector has 500 bp insert - (reply: 5)
1228. 5' RACE for very GC-rich gene - (reply: 2)
1229. Phosphorylated primers - (reply: 5)
1230. Correct orientation of the insert - (reply: 7)