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New Forum Archives (2009-)
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Molecular-Cloning
1201.
IRES1 x IRES2? -
(reply: 5)
1202.
Restriction site - efficiency of restriction
(reply: 4)
1203.
lentivirus vector problem--help help -
(reply: 6)
1204.
A problem with deleting URA3 from S. cerevisiae -
(reply: 2)
1205.
Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation
(reply: 3)
1206.
ligation - didn't got transformed colonies
(reply: 1)
1207.
What's this in my culture??? - Debris in liquid culture
(reply: 3)
1208.
assembling problem in genes - assembling problem in genes
(reply: 7)
1209.
tryptone soy broth -
(reply: 3)
1210.
no colonies - Cloning using pENTR vector, no colonies
(reply: 2)
1211.
Please help Cloning issues with smaI and pUNK1 vector -
(reply: 2)
1212.
ligation problem -
(reply: 3)
1213.
ligation question - three-point ligation
(reply: 9)
1214.
Problems cloning Hairpin vector -
(reply: 3)
1215.
request pIRES2-EGFP -
(reply: 2)
1216.
different digestion pattern for same clone -
(reply: 4)
1217.
Problem with DNA purification from agarose gel -
(reply: 3)
1218.
cloning trouble -
(reply: 15)
1219.
No band in the gel after ligation -
(reply: 6)
1220.
Troubleshooting Digest, ligation, transformation -
(reply: 7)
1221.
Ideal incubation time -
(reply: 3)
1222.
Competent DH5 alpha -
(reply: 4)
1223.
What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma
(reply: 2)
1224.
Quick ? about Enzyme Buffers -
(reply: 3)
1225.
High conc. CaCl2 inhibits electroporation?? -
(reply: 3)
1226.
Inserts too small after transformation - transformation
(reply: 3)
1227.
glycerol stocks - storage
(reply: 2)
1228.
growth of colonies after ligation -
(reply: 2)
1229.
cDNA cloning problem -
(reply: 1)
1230.
Any good way to remove DNase? -
(reply: 5)
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