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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. need help in my Fse1 restriction ezyme - (reply: 4)
1202. Insert problems with TA cloning - (reply: 1)
1203. Surface expression by tansfection of CHO - (reply: 3)
1204. please help!!! with transformation - (reply: 10)
1205. sequencing primer: T7 or T7promoter? - (reply: 4)
1206. Unstable clone? - (reply: 1)
1207. Restriction enzymes - (reply: 3)
1208. Problems facing in cloning with pBI121 vector - (reply: 1)
1209. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1210. Is my insert present??? - (reply: 17)
1211. Need help for restriction enzymes - (reply: 5)
1212. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1213. Mini prep - (reply: 4)
1214. adding koazak sequence to primer - (reply: 1)
1215. DNA ligation - (reply: 2)
1216. adding C terminal tag to reverse primer - (reply: 1)
1217. glycerol stocks - (reply: 10)
1218. doubts in sequence submission to NCBI - (reply: 2)
1219. Concentration of DNA for Transformation - (reply: 1)
1220. Restriction digest - (reply: 7)
1221. Help! Double restriction digestion failed.... - (reply: 6)
1222. Kozak sequences.. - (reply: 1)
1223. Sequence management software (for Mac) - (reply: 5)
1224. ligation for 24 hrs in 4*C? - (reply: 3)
1225. Having problem establishing stable cell line...... - (reply: 5)
1226. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)
1227. Contruction of Expression Construct - (reply: 1)
1228. problem with subcloning, please help! - (reply: 8)
1229. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
1230. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)