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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1202. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1203. problems with qiagen gel extraction kit - (reply: 7)
1204. restriction enzyme flanking sequence preferance - (reply: 2)
1205. pCDH Lentivector has 500 bp insert - (reply: 5)
1206. 5' RACE for very GC-rich gene - (reply: 2)
1207. Phosphorylated primers - (reply: 5)
1208. Correct orientation of the insert - (reply: 7)
1209. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1210. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1211. Cloning advice needed - (reply: 4)
1212. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1213. Colony Screeing without using PCR - (reply: 8)
1214. blue white screening - (reply: 3)
1215. phosphorylate dprimers for pfx do not work - (reply: 3)
1216. help - (reply: 1)
1217. problem with yeast transformation - (reply: 2)
1218. ta cloning of pfu amplified fragment - (reply: 9)
1219. how to express the gene in opposite transcriptional direction - (reply: 3)
1220. Water for electrocompetent cells - (reply: 2)
1221. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1222. Bacterial growth in agar plate with ampicillin - (reply: 4)
1223. problem in ligation and cloning - (reply: 1)
1224. Electrophoresis gel boxes and geldoc system - (reply: 2)
1225. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1226. problem with 293T cell co-transfection - (reply: 5)
1227. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
1228. how CIP treatment is done - (reply: 3)
1229. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
1230. Storage of gel extracted DNA before ligation - (reply: 3)