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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. overexpression of stable clones - (reply: 1)
1202. functionality of yeast promoter in E. coli - (reply: 1)
1203. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1204. Can't seem to do a simple cloning - (reply: 2)
1205. Histogram from Nanodrop - (reply: 1)
1206. cDNA library construction - I get only one colony (reply: 2)
1207. Cloning into pIBV5-His (not working) - (reply: 1)
1208. Addition of Restriction sites into PCR primers - (reply: 4)
1209. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1210. Streaky DNA digest - (reply: 3)
1211. TOPO TA cloning problem - (reply: 12)
1212. cloning problem - (reply: 2)
1213. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1214. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1215. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1216. Nco1 digest problem - (reply: 4)
1217. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1218. ligation - (reply: 1)
1219. pre-prepararation of competent cells - (reply: 3)
1220. different copy # multimers with linker ligation? - (reply: 2)
1221. Help ADD 3 bases to DNA Sequences - (reply: 6)
1222. My battle with BamHI - (reply: 35)
1223. serious problem in cloning - (reply: 2)
1224. What are "Ella-cre" mice? - (reply: 2)
1225. help with pCMV-myc vector - (reply: 2)
1226. Elution amount - (reply: 5)
1227. keeping restriction digested fragments at -20? - (reply: 5)
1228. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1229. re-digestion - (reply: 1)
1230. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)