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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Problem with SmaI restriction digest - (reply: 6)
1202. blunt-end / 3ŽA overhang cloning - (reply: 1)
1203. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1204. overexpression of stable clones - (reply: 1)
1205. functionality of yeast promoter in E. coli - (reply: 1)
1206. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1207. Can't seem to do a simple cloning - (reply: 2)
1208. Histogram from Nanodrop - (reply: 1)
1209. cDNA library construction - I get only one colony (reply: 2)
1210. Cloning into pIBV5-His (not working) - (reply: 1)
1211. Addition of Restriction sites into PCR primers - (reply: 4)
1212. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1213. Streaky DNA digest - (reply: 3)
1214. TOPO TA cloning problem - (reply: 12)
1215. cloning problem - (reply: 2)
1216. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1217. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1218. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1219. Nco1 digest problem - (reply: 4)
1220. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1221. ligation - (reply: 1)
1222. pre-prepararation of competent cells - (reply: 3)
1223. different copy # multimers with linker ligation? - (reply: 2)
1224. Help ADD 3 bases to DNA Sequences - (reply: 6)
1225. My battle with BamHI - (reply: 35)
1226. serious problem in cloning - (reply: 2)
1227. What are "Ella-cre" mice? - (reply: 2)
1228. help with pCMV-myc vector - (reply: 2)
1229. Elution amount - (reply: 5)
1230. keeping restriction digested fragments at -20? - (reply: 5)