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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. need help cloning cdna of a known protein - (reply: 1)
1202. restriction problem - (reply: 3)
1203. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1204. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1205. Trouble with subcloning - (reply: 6)
1206. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1207. Lb-kan plates life - How long can they be used? (reply: 2)
1208. LR reaction - Cloning into a expression vector (reply: 1)
1209. TOPO-Transformation Problem - (reply: 4)
1210. Checking Plasmid Expression - (reply: 1)
1211. designing primers for genes not sequenced yet - (reply: 2)
1212. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1213. Cloning Problem - (reply: 3)
1214. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1215. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1216. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1217. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1218. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1219. Restriction buffer composition - (reply: 2)
1220. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1221. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1222. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1223. Yet another ligation problem - (reply: 4)
1224. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1225. Promoter Cloning for Luciferase Assay - (reply: 3)
1226. Creating mutants - (reply: 3)
1227. cloning protocol - (reply: 1)
1228. Ligation Time and Temp - (reply: 4)
1229. Miniprep - (reply: 3)
1230. PCR and then ligation - (reply: 7)