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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. pre-prepararation of competent cells - (reply: 3)
1202. different copy # multimers with linker ligation? - (reply: 2)
1203. Help ADD 3 bases to DNA Sequences - (reply: 6)
1204. My battle with BamHI - (reply: 35)
1205. serious problem in cloning - (reply: 2)
1206. What are "Ella-cre" mice? - (reply: 2)
1207. help with pCMV-myc vector - (reply: 2)
1208. Elution amount - (reply: 5)
1209. keeping restriction digested fragments at -20? - (reply: 5)
1210. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1211. re-digestion - (reply: 1)
1212. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1213. Cloning using BamH1 and HindIII - (reply: 8)
1214. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1215. "competentcy" of my method - (reply: 5)
1216. Vector frameshift problem - (reply: 3)
1217. Screening colonies after transformation - (reply: 2)
1218. z competent cells - (reply: 2)
1219. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1220. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1221. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1222. plasmid extraction kit wash buffer solution - (reply: 3)
1223. molecular "terminology" - (reply: 1)
1224. NdeI digestion - Share your experience (reply: 3)
1225. Multiple bands after amping insert - (reply: 2)
1226. Good tool for sequence validation? - For Windows (reply: 2)
1227. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1228. Silencing of the first cistron - (reply: 1)
1229. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1230. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)