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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. IRES1 x IRES2? - (reply: 5)
1202. Restriction site - efficiency of restriction (reply: 4)
1203. lentivirus vector problem--help help - (reply: 6)
1204. A problem with deleting URA3 from S. cerevisiae - (reply: 2)
1205. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
1206. ligation - didn't got transformed colonies (reply: 1)
1207. What's this in my culture??? - Debris in liquid culture (reply: 3)
1208. assembling problem in genes - assembling problem in genes (reply: 7)
1209. tryptone soy broth - (reply: 3)
1210. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
1211. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
1212. ligation problem - (reply: 3)
1213. ligation question - three-point ligation (reply: 9)
1214. Problems cloning Hairpin vector - (reply: 3)
1215. request pIRES2-EGFP - (reply: 2)
1216. different digestion pattern for same clone - (reply: 4)
1217. Problem with DNA purification from agarose gel - (reply: 3)
1218. cloning trouble - (reply: 15)
1219. No band in the gel after ligation - (reply: 6)
1220. Troubleshooting Digest, ligation, transformation - (reply: 7)
1221. Ideal incubation time - (reply: 3)
1222. Competent DH5 alpha - (reply: 4)
1223. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1224. Quick ? about Enzyme Buffers - (reply: 3)
1225. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1226. Inserts too small after transformation - transformation (reply: 3)
1227. glycerol stocks - storage (reply: 2)
1228. growth of colonies after ligation - (reply: 2)
1229. cDNA cloning problem - (reply: 1)
1230. Any good way to remove DNase? - (reply: 5)