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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Stable Mammalian Expression with Retrovirus - (reply: 2)
1202. Neon Transfection System - (reply: 2)
1203. help - (reply: 5)
1204. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1205. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1206. problems with qiagen gel extraction kit - (reply: 7)
1207. restriction enzyme flanking sequence preferance - (reply: 2)
1208. pCDH Lentivector has 500 bp insert - (reply: 5)
1209. 5' RACE for very GC-rich gene - (reply: 2)
1210. Phosphorylated primers - (reply: 5)
1211. Correct orientation of the insert - (reply: 7)
1212. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1213. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1214. Cloning advice needed - (reply: 4)
1215. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1216. Colony Screeing without using PCR - (reply: 8)
1217. blue white screening - (reply: 3)
1218. phosphorylate dprimers for pfx do not work - (reply: 3)
1219. help - (reply: 1)
1220. problem with yeast transformation - (reply: 2)
1221. ta cloning of pfu amplified fragment - (reply: 9)
1222. how to express the gene in opposite transcriptional direction - (reply: 3)
1223. Water for electrocompetent cells - (reply: 2)
1224. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1225. Bacterial growth in agar plate with ampicillin - (reply: 4)
1226. problem in ligation and cloning - (reply: 1)
1227. Electrophoresis gel boxes and geldoc system - (reply: 2)
1228. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
1229. problem with 293T cell co-transfection - (reply: 5)
1230. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)