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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. best way to transport bacteria at room temperature - (reply: 5)
1202. Suicide vector - (reply: 2)
1203. Extremely small bacterial colonies after transformation - (reply: 10)
1204. tranformation efficiency - (reply: 2)
1205. transfection time - (reply: 2)
1206. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1207. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1208. Problem with SmaI restriction digest - (reply: 6)
1209. blunt-end / 3ŽA overhang cloning - (reply: 1)
1210. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1211. overexpression of stable clones - (reply: 1)
1212. functionality of yeast promoter in E. coli - (reply: 1)
1213. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1214. Can't seem to do a simple cloning - (reply: 2)
1215. Histogram from Nanodrop - (reply: 1)
1216. cDNA library construction - I get only one colony (reply: 2)
1217. Cloning into pIBV5-His (not working) - (reply: 1)
1218. Addition of Restriction sites into PCR primers - (reply: 4)
1219. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1220. Streaky DNA digest - (reply: 3)
1221. TOPO TA cloning problem - (reply: 12)
1222. cloning problem - (reply: 2)
1223. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1224. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1225. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1226. Nco1 digest problem - (reply: 4)
1227. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1228. ligation - (reply: 1)
1229. pre-prepararation of competent cells - (reply: 3)
1230. different copy # multimers with linker ligation? - (reply: 2)