Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1202. Can't seem to do a simple cloning - (reply: 2)
1203. Histogram from Nanodrop - (reply: 1)
1204. cDNA library construction - I get only one colony (reply: 2)
1205. Cloning into pIBV5-His (not working) - (reply: 1)
1206. Addition of Restriction sites into PCR primers - (reply: 4)
1207. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1208. Streaky DNA digest - (reply: 3)
1209. TOPO TA cloning problem - (reply: 12)
1210. cloning problem - (reply: 2)
1211. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1212. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1213. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1214. Nco1 digest problem - (reply: 4)
1215. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1216. ligation - (reply: 1)
1217. pre-prepararation of competent cells - (reply: 3)
1218. different copy # multimers with linker ligation? - (reply: 2)
1219. Help ADD 3 bases to DNA Sequences - (reply: 6)
1220. My battle with BamHI - (reply: 35)
1221. serious problem in cloning - (reply: 2)
1222. What are "Ella-cre" mice? - (reply: 2)
1223. help with pCMV-myc vector - (reply: 2)
1224. Elution amount - (reply: 5)
1225. keeping restriction digested fragments at -20? - (reply: 5)
1226. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1227. re-digestion - (reply: 1)
1228. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1229. Cloning using BamH1 and HindIII - (reply: 8)
1230. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)