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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1202. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1203. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1204. Restriction buffer composition - (reply: 2)
1205. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1206. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1207. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1208. Yet another ligation problem - (reply: 4)
1209. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1210. Promoter Cloning for Luciferase Assay - (reply: 3)
1211. Creating mutants - (reply: 3)
1212. cloning protocol - (reply: 1)
1213. Ligation Time and Temp - (reply: 4)
1214. Miniprep - (reply: 3)
1215. PCR and then ligation - (reply: 7)
1216. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1217. 3 way ligation help needed - (reply: 10)
1218. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1219. Cloning woes - Running out of ideas! - (reply: 7)
1220. What fields of research other than microbiology work with bacteria and - (reply: 1)
1221. How to inoculate bacteria? - (reply: 10)
1222. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1223. need help in my Fse1 restriction ezyme - (reply: 4)
1224. Insert problems with TA cloning - (reply: 1)
1225. Surface expression by tansfection of CHO - (reply: 3)
1226. please help!!! with transformation - (reply: 10)
1227. sequencing primer: T7 or T7promoter? - (reply: 4)
1228. Unstable clone? - (reply: 1)
1229. Restriction enzymes - (reply: 3)
1230. Problems facing in cloning with pBI121 vector - (reply: 1)