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Top : New Forum Archives (2009-): : Molecular-Cloning
1201. Restriction buffer composition - (reply: 2)
1202. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1203. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1204. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1205. Yet another ligation problem - (reply: 4)
1206. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1207. Promoter Cloning for Luciferase Assay - (reply: 3)
1208. Creating mutants - (reply: 3)
1209. cloning protocol - (reply: 1)
1210. Ligation Time and Temp - (reply: 4)
1211. Miniprep - (reply: 3)
1212. PCR and then ligation - (reply: 7)
1213. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1214. 3 way ligation help needed - (reply: 10)
1215. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1216. Cloning woes - Running out of ideas! - (reply: 7)
1217. What fields of research other than microbiology work with bacteria and - (reply: 1)
1218. How to inoculate bacteria? - (reply: 10)
1219. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1220. need help in my Fse1 restriction ezyme - (reply: 4)
1221. Insert problems with TA cloning - (reply: 1)
1222. Surface expression by tansfection of CHO - (reply: 3)
1223. please help!!! with transformation - (reply: 10)
1224. sequencing primer: T7 or T7promoter? - (reply: 4)
1225. Unstable clone? - (reply: 1)
1226. Restriction enzymes - (reply: 3)
1227. Problems facing in cloning with pBI121 vector - (reply: 1)
1228. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1229. Is my insert present??? - (reply: 17)
1230. Need help for restriction enzymes - (reply: 5)