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601. metagenomic library consturction - BAC vectors (reply: 1)
602. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
603. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
604. Chromosomal recombination with BL21? - (reply: 2)
605. Restriction Digestion!! - (reply: 5)
606. digestion - restriction enzymes (reply: 2)
607. How to prevent self ligation - selfligation (reply: 11)
608. what is the difference between DH10B and Top10 - (reply: 1)
609. problems encountered when working on a large plasmid - (reply: 2)
610. make a Mega plasmid mutation - (reply: 1)
611. Cloning - (reply: 1)
612. Copy-number calculation formula? - (reply: 1)
613. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
614. low melt agarose - (reply: 1)
615. restriction digest - smear - (reply: 1)
616. designing of primers - (reply: 2)
617. Propagating ccdB survival cells - (reply: 5)
618. Bicistronic vector vs "normal" vector - (reply: 5)
619. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
620. Can't seem to get insert into vector... please help - (reply: 4)
621. plasmid cloning - i need help.. can anyone answer me (reply: 5)
622. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
623. Topo cloning or pGEM-T easy ? - (reply: 3)
624. Problems with qiaquick gel purification kit - cloning (reply: 10)
625. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
626. integration of a gene per homologous recombination - (reply: 5)
627. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
628. Antarctic Phosphatase treatment - (reply: 5)
629. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
630. phage DNA - DNA wont migrate on agarose gel (reply: 3)
602. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
603. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
604. Chromosomal recombination with BL21? - (reply: 2)
605. Restriction Digestion!! - (reply: 5)
606. digestion - restriction enzymes (reply: 2)
607. How to prevent self ligation - selfligation (reply: 11)
608. what is the difference between DH10B and Top10 - (reply: 1)
609. problems encountered when working on a large plasmid - (reply: 2)
610. make a Mega plasmid mutation - (reply: 1)
611. Cloning - (reply: 1)
612. Copy-number calculation formula? - (reply: 1)
613. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
614. low melt agarose - (reply: 1)
615. restriction digest - smear - (reply: 1)
616. designing of primers - (reply: 2)
617. Propagating ccdB survival cells - (reply: 5)
618. Bicistronic vector vs "normal" vector - (reply: 5)
619. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
620. Can't seem to get insert into vector... please help - (reply: 4)
621. plasmid cloning - i need help.. can anyone answer me (reply: 5)
622. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
623. Topo cloning or pGEM-T easy ? - (reply: 3)
624. Problems with qiaquick gel purification kit - cloning (reply: 10)
625. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
626. integration of a gene per homologous recombination - (reply: 5)
627. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
628. Antarctic Phosphatase treatment - (reply: 5)
629. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
630. phage DNA - DNA wont migrate on agarose gel (reply: 3)