Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
601. an effective way to do a yeast colony pcr - (reply: 2)
602. pGEM-T Easy vector contamination - (reply: 2)
603. recover old transformed DH5 - (reply: 1)
604. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
605. oligo(dt) 15 vs random primers - (reply: 3)
606. I need bynary vector pART27 - (reply: 1)
607. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
608. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
609. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
610. cloning into Pgem t easy - (reply: 1)
611. How to get pure protein of a gene - (reply: 3)
612. How to insert genes into chromosome of E. coli - (reply: 1)
613. Gene synthesis - (reply: 2)
614. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
615. Pls help me with this plasmid!!! - (reply: 1)
616. Problems with Restriction Digest Results - (reply: 7)
617. Methods of plasmid transformation? only heat shock? - (reply: 1)
618. Digestion - (reply: 2)
619. Digestion of Genomic DNA - (reply: 1)
620. Isolation of a Gene of Interest for Subcloning - (reply: 8)
621. E.coli grow on neg. control - (reply: 1)
622. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
623. Need help troubleshooting digest. - (reply: 1)
624. Competent cells question: JM101 vs DH5alpha - (reply: 2)
625. How to avoid plasmid instability - (reply: 4)
626. Question Help - (reply: 1)
627. Shear-induced damage of plasmid by vortexing - (reply: 1)
628. Problems with blunt end ligation - (reply: 3)
629. Problem with Transforming E. coli DH10Bac - (reply: 8)
630. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)