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Top : New Forum Archives (2009-): : Molecular-Cloning
601. plasmid tetR and tet promoter - (reply: 2)
602. unable to amplify an infectious retrovirus clone - (reply: 4)
603. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
604. No bands at all after double digestion - (reply: 1)
605. Digest for insert orientation - (reply: 4)
606. Colonies from transformation plate are not growing...:( - (reply: 5)
607. Problem with ligating a small insert (LoxP site) - (reply: 5)
608. Is the genomic DNA similar among different cell lines? - (reply: 4)
609. molecular cloning - (reply: 3)
610. Swich selection in plasmid - (reply: 3)
611. Sub-cloning and transformation - (reply: 17)
612. cloning without phosphorylation - (reply: 7)
613. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
614. A good vector to make gene library? - (reply: 5)
615. problems with midiprep - (reply: 3)
616. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
617. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
618. 900bp insertion into PIRESnEO,problem - (reply: 2)
619. fusion of cDNA expressing protein with gfp - (reply: 1)
620. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
621. Problem with plasmids transformation - (reply: 6)
622. Cloning genetic sequences of proteins. - (reply: 1)
623. problem of restriction enzyme digestion - (reply: 3)
624. Site-directed, PCR works but no colonies - (reply: 2)
625. Simoltanious Multi-restriction digestion - (reply: 3)
626. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
627. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
628. Restriction digestion problems - (reply: 3)
629. should I do blunt end my pcr product before ligation - (reply: 2)
630. Maxiprep of freeze/thawed pellet. - (reply: 1)