Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
601. Heat inactivate ligase - (reply: 5)
602. DMSO stock of overnight bacterial cultures - (reply: 1)
603. Recommended competent cell for transformation of large plasmids? - (reply: 4)
604. Prolem in screening of hygromycin concentration - (reply: 1)
605. Primers for Introduction of new restriction sites to a vector - (reply: 3)
606. problem in cloning involving partial digestion - (reply: 1)
607. Why perform restriction enzyme digestion again? - (reply: 1)
608. Cotransient Transfections DNA Requirements - (reply: 3)
609. which software to draw the scheme of cloning strategy - (reply: 5)
610. Help with restriction analysis - (reply: 1)
611. Sequencing of PCR product - (reply: 4)
612. KanR sequence - (reply: 2)
613. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
614. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
615. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
616. Plasmid digestion after transformation - (reply: 4)
617. Blunt end ligation insert:vector ratio - (reply: 1)
618. PCRed on restriction sites -- how do I purify it? - (reply: 8)
619. Puzzle about plamid and its digest, plz help - (reply: 8)
620. Smear above plasmid dna - (reply: 3)
621. restriction digestion buffers contaminated with dnase - (reply: 3)
622. Bacterial Induction - (reply: 1)
623. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
624. smear restriction digest - (reply: 1)
625. How to set the Sonicator to 20%amplitude? - (reply: 3)
626. No DNA after midiprep - (reply: 2)
627. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
628. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
629. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
630. Impact of primer sequence on TA cloning - (reply: 3)