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Top : New Forum Archives (2009-): : Molecular-Cloning
601. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
602. Problem with plasmids transformation - (reply: 6)
603. Cloning genetic sequences of proteins. - (reply: 1)
604. problem of restriction enzyme digestion - (reply: 3)
605. Site-directed, PCR works but no colonies - (reply: 2)
606. Simoltanious Multi-restriction digestion - (reply: 3)
607. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
608. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
609. Restriction digestion problems - (reply: 3)
610. should I do blunt end my pcr product before ligation - (reply: 2)
611. Maxiprep of freeze/thawed pellet. - (reply: 1)
612. Digest with AarI and Pml I - (reply: 1)
613. Help with CGL gene cloning problems - (reply: 1)
614. screening for GPF - (reply: 10)
615. Creating a pGEM-T easy + insert sequence map - (reply: 1)
616. where to get vector overexpressing caveolin-1? - (reply: 2)
617. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
618. pINDUCER series - (reply: 1)
619. LZRS vector details - (reply: 1)
620. 12.4kb vector 6.7kb insert - (reply: 2)
621. Stable transfection - (reply: 2)
622. Using normal competent cells in electroporation method? - (reply: 1)
623. an effective way to do a yeast colony pcr - (reply: 2)
624. pGEM-T Easy vector contamination - (reply: 2)
625. recover old transformed DH5 - (reply: 1)
626. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
627. oligo(dt) 15 vs random primers - (reply: 3)
628. I need bynary vector pART27 - (reply: 1)
629. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
630. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)