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Top : New Forum Archives (2009-): : Molecular-Cloning
601. making recombinant protein - (reply: 2)
602. Confirming insert???? - (reply: 1)
603. how to make a recombinant protein with signal peptide - (reply: 1)
604. How to add restriction enzyme site into the DNA fragment - (reply: 3)
605. Issues with ligation - (reply: 2)
606. Ligation of double insert into pET31 failing - (reply: 1)
607. Homemade MiniPreps for Plasmid Purification - (reply: 1)
608. Agar has dried out during cultivation - (reply: 1)
609. Gateway cloning vector compatibility - (reply: 2)
610. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
611. Cloning of IL8-promoter - (reply: 3)
612. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
613. Template DNA (plasmid) concentration for PCR - (reply: 4)
614. Ligation of 16kb PCR product - (reply: 5)
615. stable transfection - (reply: 1)
616. How to choose the right plasmids for certain types of cell? - (reply: 1)
617. Ligation needed after SDM with PfuX? - (reply: 3)
618. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
619. Cloning promoter (overlap with another gene!) - (reply: 3)
620. bacterial transfeomation - (reply: 2)
621. Loss of insert? - (reply: 1)
622. Sequencing trouble - (reply: 7)
623. Platinum-A cell trasnfection toruble - (reply: 1)
624. Cloning a promoter - (reply: 2)
625. pcDNA 3.1 His Expression - (reply: 2)
626. Cloning two fragments into a vector - (reply: 3)
627. confirmation of insert in to vector - (reply: 3)
628. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
629. Question about Molar ratio - (reply: 4)
630. Ligation problem - (reply: 16)