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Top : New Forum Archives (2009-): : Molecular-Cloning
601. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
602. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
603. Restriction digestion problems - (reply: 3)
604. should I do blunt end my pcr product before ligation - (reply: 2)
605. Maxiprep of freeze/thawed pellet. - (reply: 1)
606. Digest with AarI and Pml I - (reply: 1)
607. Help with CGL gene cloning problems - (reply: 1)
608. screening for GPF - (reply: 10)
609. Creating a pGEM-T easy + insert sequence map - (reply: 1)
610. where to get vector overexpressing caveolin-1? - (reply: 2)
611. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
612. pINDUCER series - (reply: 1)
613. LZRS vector details - (reply: 1)
614. 12.4kb vector 6.7kb insert - (reply: 2)
615. Stable transfection - (reply: 2)
616. Using normal competent cells in electroporation method? - (reply: 1)
617. an effective way to do a yeast colony pcr - (reply: 2)
618. pGEM-T Easy vector contamination - (reply: 2)
619. recover old transformed DH5 - (reply: 1)
620. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
621. oligo(dt) 15 vs random primers - (reply: 3)
622. I need bynary vector pART27 - (reply: 1)
623. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
624. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
625. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
626. cloning into Pgem t easy - (reply: 1)
627. How to get pure protein of a gene - (reply: 3)
628. How to insert genes into chromosome of E. coli - (reply: 1)
629. Gene synthesis - (reply: 2)
630. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)