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Top : New Forum Archives (2009-): : Molecular-Cloning
601. fusion of cDNA expressing protein with gfp - (reply: 1)
602. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
603. Problem with plasmids transformation - (reply: 6)
604. Cloning genetic sequences of proteins. - (reply: 1)
605. problem of restriction enzyme digestion - (reply: 3)
606. Site-directed, PCR works but no colonies - (reply: 2)
607. Simoltanious Multi-restriction digestion - (reply: 3)
608. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
609. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
610. Restriction digestion problems - (reply: 3)
611. should I do blunt end my pcr product before ligation - (reply: 2)
612. Maxiprep of freeze/thawed pellet. - (reply: 1)
613. Digest with AarI and Pml I - (reply: 1)
614. Help with CGL gene cloning problems - (reply: 1)
615. screening for GPF - (reply: 10)
616. Creating a pGEM-T easy + insert sequence map - (reply: 1)
617. where to get vector overexpressing caveolin-1? - (reply: 2)
618. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
619. pINDUCER series - (reply: 1)
620. LZRS vector details - (reply: 1)
621. 12.4kb vector 6.7kb insert - (reply: 2)
622. Stable transfection - (reply: 2)
623. Using normal competent cells in electroporation method? - (reply: 1)
624. an effective way to do a yeast colony pcr - (reply: 2)
625. pGEM-T Easy vector contamination - (reply: 2)
626. recover old transformed DH5 - (reply: 1)
627. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
628. oligo(dt) 15 vs random primers - (reply: 3)
629. I need bynary vector pART27 - (reply: 1)
630. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)