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Top : New Forum Archives (2009-): : Molecular-Cloning
601. 900bp insertion into PIRESnEO,problem - (reply: 2)
602. fusion of cDNA expressing protein with gfp - (reply: 1)
603. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
604. Problem with plasmids transformation - (reply: 6)
605. Cloning genetic sequences of proteins. - (reply: 1)
606. problem of restriction enzyme digestion - (reply: 3)
607. Site-directed, PCR works but no colonies - (reply: 2)
608. Simoltanious Multi-restriction digestion - (reply: 3)
609. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
610. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
611. Restriction digestion problems - (reply: 3)
612. should I do blunt end my pcr product before ligation - (reply: 2)
613. Maxiprep of freeze/thawed pellet. - (reply: 1)
614. Digest with AarI and Pml I - (reply: 1)
615. Help with CGL gene cloning problems - (reply: 1)
616. screening for GPF - (reply: 10)
617. Creating a pGEM-T easy + insert sequence map - (reply: 1)
618. where to get vector overexpressing caveolin-1? - (reply: 2)
619. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
620. pINDUCER series - (reply: 1)
621. LZRS vector details - (reply: 1)
622. 12.4kb vector 6.7kb insert - (reply: 2)
623. Stable transfection - (reply: 2)
624. Using normal competent cells in electroporation method? - (reply: 1)
625. an effective way to do a yeast colony pcr - (reply: 2)
626. pGEM-T Easy vector contamination - (reply: 2)
627. recover old transformed DH5 - (reply: 1)
628. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
629. oligo(dt) 15 vs random primers - (reply: 3)
630. I need bynary vector pART27 - (reply: 1)