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Top : New Forum Archives (2009-): : Molecular-Cloning
601. Sub-cloning and transformation - (reply: 17)
602. cloning without phosphorylation - (reply: 7)
603. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
604. A good vector to make gene library? - (reply: 5)
605. problems with midiprep - (reply: 3)
606. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
607. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
608. 900bp insertion into PIRESnEO,problem - (reply: 2)
609. fusion of cDNA expressing protein with gfp - (reply: 1)
610. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
611. Problem with plasmids transformation - (reply: 6)
612. Cloning genetic sequences of proteins. - (reply: 1)
613. problem of restriction enzyme digestion - (reply: 3)
614. Site-directed, PCR works but no colonies - (reply: 2)
615. Simoltanious Multi-restriction digestion - (reply: 3)
616. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
617. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
618. Restriction digestion problems - (reply: 3)
619. should I do blunt end my pcr product before ligation - (reply: 2)
620. Maxiprep of freeze/thawed pellet. - (reply: 1)
621. Digest with AarI and Pml I - (reply: 1)
622. Help with CGL gene cloning problems - (reply: 1)
623. screening for GPF - (reply: 10)
624. Creating a pGEM-T easy + insert sequence map - (reply: 1)
625. where to get vector overexpressing caveolin-1? - (reply: 2)
626. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
627. pINDUCER series - (reply: 1)
628. LZRS vector details - (reply: 1)
629. 12.4kb vector 6.7kb insert - (reply: 2)
630. Stable transfection - (reply: 2)