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Top : New Forum Archives (2009-): : Molecular-Cloning
601. problem of restriction enzyme digestion - (reply: 3)
602. Site-directed, PCR works but no colonies - (reply: 2)
603. Simoltanious Multi-restriction digestion - (reply: 3)
604. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
605. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
606. Restriction digestion problems - (reply: 3)
607. should I do blunt end my pcr product before ligation - (reply: 2)
608. Maxiprep of freeze/thawed pellet. - (reply: 1)
609. Digest with AarI and Pml I - (reply: 1)
610. Help with CGL gene cloning problems - (reply: 1)
611. screening for GPF - (reply: 10)
612. Creating a pGEM-T easy + insert sequence map - (reply: 1)
613. where to get vector overexpressing caveolin-1? - (reply: 2)
614. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
615. pINDUCER series - (reply: 1)
616. LZRS vector details - (reply: 1)
617. 12.4kb vector 6.7kb insert - (reply: 2)
618. Stable transfection - (reply: 2)
619. Using normal competent cells in electroporation method? - (reply: 1)
620. an effective way to do a yeast colony pcr - (reply: 2)
621. pGEM-T Easy vector contamination - (reply: 2)
622. recover old transformed DH5 - (reply: 1)
623. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
624. oligo(dt) 15 vs random primers - (reply: 3)
625. I need bynary vector pART27 - (reply: 1)
626. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
627. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
628. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
629. cloning into Pgem t easy - (reply: 1)
630. How to get pure protein of a gene - (reply: 3)