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Top : New Forum Archives (2009-): : Molecular-Cloning
601. Three-way ligation in two steps? - (reply: 3)
602. How does circular, uncut pGEM-T migrate? - (reply: 2)
603. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
604. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
605. Cloning App Released from Life Technologies - (reply: 2)
606. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
607. Dissolve IPTG in EtOH - (reply: 3)
608. problem with transient transfection - (reply: 6)
609. Recombinant construct looks smaller than empty vector - (reply: 3)
610. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
611. hygromycin concentration for e-coli selection - (reply: 1)
612. orientation of promoter into viral construct - (reply: 1)
613. problems with PCR confirmation of insert . HELP ! - (reply: 1)
614. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
615. Loss of promoter during ligation - (reply: 2)
616. Enzyme restriction - (reply: 8)
617. Colony PCR Problem!!!! - (reply: 5)
618. Removing unwanted sequence from pUC19 - (reply: 4)
619. transformation fails, bacteria grows in control plate - (reply: 6)
620. competentce cell, cloning - (reply: 2)
621. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
622. co-transformation - (reply: 3)
623. pcr clean-up fail? - (reply: 1)
624. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
625. sequential cloning of multiple PCR products - how to do? (reply: 3)
626. Cloning large gene - (reply: 3)
627. Glycosilated peptide's cDNA cloning - (reply: 3)
628. pWM91 cloning problem - (reply: 4)
629. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
630. Transformation - Transformation (reply: 1)