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Top : New Forum Archives (2009-): : Molecular-Cloning
601. Fixing SNPs after cloning - (reply: 5)
602. Electroporated plasmid loses the insert - (reply: 8)
603. Recombination due to overselective plate? - (reply: 11)
604. Help with ligation - (reply: 2)
605. emty vector cloning - (reply: 3)
606. RE site on complimentary strand of plasmid? - (reply: 1)
607. LENTIVIRUS transfection efficiency - (reply: 1)
608. Double digest after cloning doesn't give gene - (reply: 6)
609. Blunt end PCR product ligation - (reply: 1)
610. Screening for clones - (reply: 2)
611. Plasmid isolation Qiagen - (reply: 2)
612. Restriction Site Destruction - (reply: 1)
613. The copy number of pPICZ alpha A - (reply: 6)
614. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
615. need help with subcloning !! - (reply: 2)
616. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
617. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
618. cdna library. - (reply: 2)
619. Help in RE digestion of vector - (reply: 5)
620. bacterial transformation problem - (reply: 2)
621. Transformation - (reply: 3)
622. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
623. What does vortexing do in transformation? - (reply: 1)
624. failure in miniprep - (reply: 1)
625. Host cells for transformation - (reply: 3)
626. making recombinant protein - (reply: 2)
627. Confirming insert???? - (reply: 1)
628. how to make a recombinant protein with signal peptide - (reply: 1)
629. How to add restriction enzyme site into the DNA fragment - (reply: 3)
630. Issues with ligation - (reply: 2)