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Top : New Forum Archives (2009-): : Molecular-Cloning
601. pINDUCER series - (reply: 1)
602. LZRS vector details - (reply: 1)
603. 12.4kb vector 6.7kb insert - (reply: 2)
604. Stable transfection - (reply: 2)
605. Using normal competent cells in electroporation method? - (reply: 1)
606. an effective way to do a yeast colony pcr - (reply: 2)
607. pGEM-T Easy vector contamination - (reply: 2)
608. recover old transformed DH5 - (reply: 1)
609. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
610. oligo(dt) 15 vs random primers - (reply: 3)
611. I need bynary vector pART27 - (reply: 1)
612. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
613. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
614. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
615. cloning into Pgem t easy - (reply: 1)
616. How to get pure protein of a gene - (reply: 3)
617. How to insert genes into chromosome of E. coli - (reply: 1)
618. Gene synthesis - (reply: 2)
619. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
620. Pls help me with this plasmid!!! - (reply: 1)
621. Problems with Restriction Digest Results - (reply: 7)
622. Methods of plasmid transformation? only heat shock? - (reply: 1)
623. Digestion - (reply: 2)
624. Digestion of Genomic DNA - (reply: 1)
625. Isolation of a Gene of Interest for Subcloning - (reply: 8)
626. E.coli grow on neg. control - (reply: 1)
627. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
628. Need help troubleshooting digest. - (reply: 1)
629. Competent cells question: JM101 vs DH5alpha - (reply: 2)
630. How to avoid plasmid instability - (reply: 4)