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Top : New Forum Archives (2009-): : Molecular-Cloning
601. Swich selection in plasmid - (reply: 3)
602. Sub-cloning and transformation - (reply: 17)
603. cloning without phosphorylation - (reply: 7)
604. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
605. A good vector to make gene library? - (reply: 5)
606. problems with midiprep - (reply: 3)
607. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
608. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
609. 900bp insertion into PIRESnEO,problem - (reply: 2)
610. fusion of cDNA expressing protein with gfp - (reply: 1)
611. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
612. Problem with plasmids transformation - (reply: 6)
613. Cloning genetic sequences of proteins. - (reply: 1)
614. problem of restriction enzyme digestion - (reply: 3)
615. Site-directed, PCR works but no colonies - (reply: 2)
616. Simoltanious Multi-restriction digestion - (reply: 3)
617. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
618. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
619. Restriction digestion problems - (reply: 3)
620. should I do blunt end my pcr product before ligation - (reply: 2)
621. Maxiprep of freeze/thawed pellet. - (reply: 1)
622. Digest with AarI and Pml I - (reply: 1)
623. Help with CGL gene cloning problems - (reply: 1)
624. screening for GPF - (reply: 10)
625. Creating a pGEM-T easy + insert sequence map - (reply: 1)
626. where to get vector overexpressing caveolin-1? - (reply: 2)
627. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
628. pINDUCER series - (reply: 1)
629. LZRS vector details - (reply: 1)
630. 12.4kb vector 6.7kb insert - (reply: 2)