Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1081. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1082. hENT1 - (reply: 1)
1083. digestion of insert twice and ligation of 3 bits - (reply: 6)
1084. blunt end ligation problem - Self ligation and truncate (reply: 1)
1085. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1086. neb ligase - (reply: 2)
1087. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1088. problem about vector Dephosphorylation - (reply: 2)
1089. Early transfection - (reply: 6)
1090. Cloning: Randomly recombined Plasmids? - (reply: 6)
1091. problem about fused protein - problem about fused protein (reply: 2)
1092. Bacteria growing on plate but not liquid media - (reply: 8)
1093. What means BamHI-linkered - (reply: 1)
1094. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1095. Problems with Dpn1 - (reply: 5)
1096. forgot to flip the plate over - but still got colonies (reply: 2)
1097. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1098. Yet another ligation problem - (reply: 6)
1099. Cloning problems - (reply: 7)
1100. Bacteria and Amaxa - (reply: 2)
1101. Problem in Ligation - (reply: 12)
1102. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1103. multiway ligation - (reply: 6)
1104. Plasmid size problems! - (reply: 3)
1105. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1106. ligation problem - (reply: 6)
1107. Failed Ligation - (reply: 2)
1108. cloning of GroEL - (reply: 3)
1109. Ligation problem - (reply: 9)
1110. Problems with High Background with Gateway Cloning - (reply: 1)