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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1082. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1083. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1084. mammalian expression vector - (reply: 2)
1085. Electroporation doesn't like my plasmid??? - (reply: 4)
1086. low yeild fo RNA after invitro transcription - (reply: 4)
1087. No expresion of transgene - Retroviral vector (reply: 1)
1088. plasmids - plasmids for stable cell line construction (reply: 2)
1089. empty vectors more than the insert - (reply: 6)
1090. Restriction enzyme digestion problem - (reply: 2)
1091. low copy plasmid - how to improve the yield? (reply: 1)
1092. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1093. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1094. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1095. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1096. Cloning Help!! - It is getting very annoying... (reply: 13)
1097. restriction digestion - (reply: 7)
1098. sequence errors created during cloning - (reply: 6)
1099. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1100. the pyrithiamine - (reply: 5)
1101. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1102. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1103. What is the role of DMSO in yeast transformation - (reply: 1)
1104. ligation problem - (reply: 3)
1105. After Transfection - (reply: 1)
1106. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1107. Help cloning large insert into large vector - (reply: 7)
1108. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1109. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1110. hENT1 - (reply: 1)