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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1082. selecting recombinants - (reply: 1)
1083. colony PCR for subcloning gene - (reply: 1)
1084. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1085. cloning of a 20 kb insert - (reply: 2)
1086. DNA quantification after gel purification - (reply: 6)
1087. cloning of an operon - (reply: 2)
1088. storing plasmid - -80degrees (reply: 4)
1089. verifying a positive transformant - (reply: 3)
1090. Need advice on cloning very small insert into a vector - (reply: 3)
1091. Cloning problems i.e. Ligation - (reply: 2)
1092. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1093. Radioactive carbon - (reply: 3)
1094. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1095. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1096. Digesting large amount of plasmid - (reply: 4)
1097. cleanup of digestion before ligation - (reply: 6)
1098. transformation efficiency - (reply: 7)
1099. transformed bacteria doesnt grow - (reply: 1)
1100. TOPO Cloning - (reply: 5)
1101. blunt ligation - (reply: 4)
1102. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1103. About Laminin 5'UTR 's function - (reply: 1)
1104. Vectors with special MCS - (reply: 5)
1105. Cloning <100 bp fragments into a vector - (reply: 7)
1106. Help! my plasmid size changed after ligation - (reply: 5)
1107. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1108. Restriction digestion of gDNA for phage library - (reply: 5)
1109. how i can get insert back? - (reply: 1)
1110. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)