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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1082. cloning of a 20 kb insert - (reply: 2)
1083. DNA quantification after gel purification - (reply: 6)
1084. cloning of an operon - (reply: 2)
1085. storing plasmid - -80degrees (reply: 4)
1086. verifying a positive transformant - (reply: 3)
1087. Need advice on cloning very small insert into a vector - (reply: 3)
1088. Cloning problems i.e. Ligation - (reply: 2)
1089. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1090. Radioactive carbon - (reply: 3)
1091. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1092. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1093. Digesting large amount of plasmid - (reply: 4)
1094. cleanup of digestion before ligation - (reply: 6)
1095. transformation efficiency - (reply: 7)
1096. transformed bacteria doesnt grow - (reply: 1)
1097. TOPO Cloning - (reply: 5)
1098. blunt ligation - (reply: 4)
1099. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1100. About Laminin 5'UTR 's function - (reply: 1)
1101. Vectors with special MCS - (reply: 5)
1102. Cloning <100 bp fragments into a vector - (reply: 7)
1103. Help! my plasmid size changed after ligation - (reply: 5)
1104. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1105. Restriction digestion of gDNA for phage library - (reply: 5)
1106. how i can get insert back? - (reply: 1)
1107. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1108. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1109. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1110. mammalian expression vector - (reply: 2)