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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1082. BstUI digestion - (reply: 1)
1083. I have cloning problems with pGEM-T Easy - (reply: 5)
1084. CaCl2 for making competent cells - (reply: 3)
1085. best way to transport bacteria at room temperature - (reply: 5)
1086. Suicide vector - (reply: 2)
1087. Extremely small bacterial colonies after transformation - (reply: 10)
1088. tranformation efficiency - (reply: 2)
1089. transfection time - (reply: 2)
1090. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1091. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1092. Problem with SmaI restriction digest - (reply: 6)
1093. blunt-end / 3ŽA overhang cloning - (reply: 1)
1094. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1095. overexpression of stable clones - (reply: 1)
1096. functionality of yeast promoter in E. coli - (reply: 1)
1097. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1098. Can't seem to do a simple cloning - (reply: 2)
1099. Histogram from Nanodrop - (reply: 1)
1100. cDNA library construction - I get only one colony (reply: 2)
1101. Cloning into pIBV5-His (not working) - (reply: 1)
1102. Addition of Restriction sites into PCR primers - (reply: 4)
1103. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1104. Streaky DNA digest - (reply: 3)
1105. TOPO TA cloning problem - (reply: 12)
1106. cloning problem - (reply: 2)
1107. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1108. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1109. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1110. Nco1 digest problem - (reply: 4)