Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1081. cleanup of digestion before ligation - (reply: 6)
1082. transformation efficiency - (reply: 7)
1083. transformed bacteria doesnt grow - (reply: 1)
1084. TOPO Cloning - (reply: 5)
1085. blunt ligation - (reply: 4)
1086. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1087. About Laminin 5'UTR 's function - (reply: 1)
1088. Vectors with special MCS - (reply: 5)
1089. Cloning <100 bp fragments into a vector - (reply: 7)
1090. Help! my plasmid size changed after ligation - (reply: 5)
1091. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1092. Restriction digestion of gDNA for phage library - (reply: 5)
1093. how i can get insert back? - (reply: 1)
1094. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1095. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1096. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1097. mammalian expression vector - (reply: 2)
1098. Electroporation doesn't like my plasmid??? - (reply: 4)
1099. low yeild fo RNA after invitro transcription - (reply: 4)
1100. No expresion of transgene - Retroviral vector (reply: 1)
1101. plasmids - plasmids for stable cell line construction (reply: 2)
1102. empty vectors more than the insert - (reply: 6)
1103. Restriction enzyme digestion problem - (reply: 2)
1104. low copy plasmid - how to improve the yield? (reply: 1)
1105. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1106. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1107. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1108. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1109. Cloning Help!! - It is getting very annoying... (reply: 13)
1110. restriction digestion - (reply: 7)