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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. empty vectors more than the insert - (reply: 6)
1082. Restriction enzyme digestion problem - (reply: 2)
1083. low copy plasmid - how to improve the yield? (reply: 1)
1084. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1085. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1086. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1087. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1088. Cloning Help!! - It is getting very annoying... (reply: 13)
1089. restriction digestion - (reply: 7)
1090. sequence errors created during cloning - (reply: 6)
1091. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1092. the pyrithiamine - (reply: 5)
1093. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1094. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1095. What is the role of DMSO in yeast transformation - (reply: 1)
1096. ligation problem - (reply: 3)
1097. After Transfection - (reply: 1)
1098. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1099. Help cloning large insert into large vector - (reply: 7)
1100. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1101. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1102. hENT1 - (reply: 1)
1103. digestion of insert twice and ligation of 3 bits - (reply: 6)
1104. blunt end ligation problem - Self ligation and truncate (reply: 1)
1105. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1106. neb ligase - (reply: 2)
1107. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1108. problem about vector Dephosphorylation - (reply: 2)
1109. Early transfection - (reply: 6)
1110. Cloning: Randomly recombined Plasmids? - (reply: 6)