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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. TOPO Cloning - (reply: 5)
1082. blunt ligation - (reply: 4)
1083. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1084. About Laminin 5'UTR 's function - (reply: 1)
1085. Vectors with special MCS - (reply: 5)
1086. Cloning <100 bp fragments into a vector - (reply: 7)
1087. Help! my plasmid size changed after ligation - (reply: 5)
1088. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1089. Restriction digestion of gDNA for phage library - (reply: 5)
1090. how i can get insert back? - (reply: 1)
1091. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1092. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1093. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1094. mammalian expression vector - (reply: 2)
1095. Electroporation doesn't like my plasmid??? - (reply: 4)
1096. low yeild fo RNA after invitro transcription - (reply: 4)
1097. No expresion of transgene - Retroviral vector (reply: 1)
1098. plasmids - plasmids for stable cell line construction (reply: 2)
1099. empty vectors more than the insert - (reply: 6)
1100. Restriction enzyme digestion problem - (reply: 2)
1101. low copy plasmid - how to improve the yield? (reply: 1)
1102. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1103. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1104. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1105. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1106. Cloning Help!! - It is getting very annoying... (reply: 13)
1107. restriction digestion - (reply: 7)
1108. sequence errors created during cloning - (reply: 6)
1109. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1110. the pyrithiamine - (reply: 5)