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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. forgot to flip the plate over - but still got colonies (reply: 2)
1082. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1083. Yet another ligation problem - (reply: 6)
1084. Cloning problems - (reply: 7)
1085. Bacteria and Amaxa - (reply: 2)
1086. Problem in Ligation - (reply: 12)
1087. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1088. multiway ligation - (reply: 6)
1089. Plasmid size problems! - (reply: 3)
1090. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1091. ligation problem - (reply: 6)
1092. Failed Ligation - (reply: 2)
1093. cloning of GroEL - (reply: 3)
1094. Ligation problem - (reply: 9)
1095. Problems with High Background with Gateway Cloning - (reply: 1)
1096. Mutagenesis troubles - (reply: 2)
1097. ligation/transformation failed - (reply: 7)
1098. Transfecting primary cells with plasmid - (reply: 1)
1099. Extra bands after QIAGEN spin miniprep - (reply: 3)
1100. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1101. The use of hygromycin - (reply: 2)
1102. QUESTION about cloning - (reply: 5)
1103. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1104. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1105. Ligation screening - (reply: 4)
1106. Double digestion and ligation problem - (reply: 1)
1107. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1108. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1109. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1110. CMV promoter - (reply: 2)