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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1082. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1083. Cloning Help!! - It is getting very annoying... (reply: 13)
1084. restriction digestion - (reply: 7)
1085. sequence errors created during cloning - (reply: 6)
1086. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1087. the pyrithiamine - (reply: 5)
1088. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1089. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1090. What is the role of DMSO in yeast transformation - (reply: 1)
1091. ligation problem - (reply: 3)
1092. After Transfection - (reply: 1)
1093. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1094. Help cloning large insert into large vector - (reply: 7)
1095. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1096. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1097. hENT1 - (reply: 1)
1098. digestion of insert twice and ligation of 3 bits - (reply: 6)
1099. blunt end ligation problem - Self ligation and truncate (reply: 1)
1100. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1101. neb ligase - (reply: 2)
1102. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1103. problem about vector Dephosphorylation - (reply: 2)
1104. Early transfection - (reply: 6)
1105. Cloning: Randomly recombined Plasmids? - (reply: 6)
1106. problem about fused protein - problem about fused protein (reply: 2)
1107. Bacteria growing on plate but not liquid media - (reply: 8)
1108. What means BamHI-linkered - (reply: 1)
1109. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1110. Problems with Dpn1 - (reply: 5)