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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. "competentcy" of my method - (reply: 5)
1082. Vector frameshift problem - (reply: 3)
1083. Screening colonies after transformation - (reply: 2)
1084. z competent cells - (reply: 2)
1085. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1086. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1087. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1088. plasmid extraction kit wash buffer solution - (reply: 3)
1089. molecular "terminology" - (reply: 1)
1090. NdeI digestion - Share your experience (reply: 3)
1091. Multiple bands after amping insert - (reply: 2)
1092. Good tool for sequence validation? - For Windows (reply: 2)
1093. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1094. Silencing of the first cistron - (reply: 1)
1095. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1096. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1097. Stable Mammalian Expression with Retrovirus - (reply: 2)
1098. Neon Transfection System - (reply: 2)
1099. help - (reply: 5)
1100. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1101. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1102. problems with qiagen gel extraction kit - (reply: 7)
1103. restriction enzyme flanking sequence preferance - (reply: 2)
1104. pCDH Lentivector has 500 bp insert - (reply: 5)
1105. 5' RACE for very GC-rich gene - (reply: 2)
1106. Phosphorylated primers - (reply: 5)
1107. Correct orientation of the insert - (reply: 7)
1108. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1109. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1110. Cloning advice needed - (reply: 4)