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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. Addition of Restriction sites into PCR primers - (reply: 4)
1082. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1083. Streaky DNA digest - (reply: 3)
1084. TOPO TA cloning problem - (reply: 12)
1085. cloning problem - (reply: 2)
1086. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1087. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
1088. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
1089. Nco1 digest problem - (reply: 4)
1090. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1091. ligation - (reply: 1)
1092. pre-prepararation of competent cells - (reply: 3)
1093. different copy # multimers with linker ligation? - (reply: 2)
1094. Help ADD 3 bases to DNA Sequences - (reply: 6)
1095. My battle with BamHI - (reply: 34)
1096. serious problem in cloning - (reply: 2)
1097. What are "Ella-cre" mice? - (reply: 2)
1098. help with pCMV-myc vector - (reply: 2)
1099. Elution amount - (reply: 5)
1100. keeping restriction digested fragments at -20? - (reply: 5)
1101. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1102. re-digestion - (reply: 1)
1103. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1104. Cloning using BamH1 and HindIII - (reply: 8)
1105. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1106. "competentcy" of my method - (reply: 5)
1107. Vector frameshift problem - (reply: 3)
1108. Screening colonies after transformation - (reply: 2)
1109. z competent cells - (reply: 2)
1110. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)