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Top : New Forum Archives (2009-): : Molecular-Cloning
1081. blunt ligation - (reply: 4)
1082. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1083. About Laminin 5'UTR 's function - (reply: 1)
1084. Vectors with special MCS - (reply: 5)
1085. Cloning <100 bp fragments into a vector - (reply: 7)
1086. Help! my plasmid size changed after ligation - (reply: 5)
1087. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1088. Restriction digestion of gDNA for phage library - (reply: 5)
1089. how i can get insert back? - (reply: 1)
1090. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1091. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1092. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1093. mammalian expression vector - (reply: 2)
1094. Electroporation doesn't like my plasmid??? - (reply: 4)
1095. low yeild fo RNA after invitro transcription - (reply: 4)
1096. No expresion of transgene - Retroviral vector (reply: 1)
1097. plasmids - plasmids for stable cell line construction (reply: 2)
1098. empty vectors more than the insert - (reply: 6)
1099. Restriction enzyme digestion problem - (reply: 2)
1100. low copy plasmid - how to improve the yield? (reply: 1)
1101. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1102. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1103. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1104. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1105. Cloning Help!! - It is getting very annoying... (reply: 13)
1106. restriction digestion - (reply: 7)
1107. sequence errors created during cloning - (reply: 6)
1108. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1109. the pyrithiamine - (reply: 5)
1110. Please advise on my transgene strategy :) - Thanks! (reply: 4)