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1081. wrong bands after T-vector cloning - (reply: 3)
1082. urgent help regarding wanner method trouble shooting - (reply: 2)
1083. two step digestion - (reply: 6)
1084. Storage of invitrogen top10 cells - (reply: 11)
1085. Self-Ligation of digested vector - (reply: 2)
1086. Help with cloning - (reply: 6)
1087. Ligation issue: Does vector size matter? - (reply: 10)
1088. Help for T-A cloning - (reply: 15)
1089. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1090. Some colonies, but not enough... - (reply: 7)
1091. transformation problem - (reply: 3)
1092. Plasmid sequence - (reply: 1)
1093. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1094. help wih the ligation - (reply: 7)
1095. PCR screening of transformed bacterial colony - (reply: 6)
1096. No colony for oligo cloning - (reply: 9)
1097. cloning - affect on c-terminal (reply: 1)
1098. Growing of TOP10 chemically competent cells - (reply: 4)
1099. Stable Cell Selection with Neomycin - (reply: 4)
1100. lpzzzzz that is so important to my PhD. - (reply: 1)
1101. need guidence for cdna library - screen library (reply: 5)
1102. No product after restriction digestion - (reply: 26)
1103. protein overexpression question. - (reply: 4)
1104. Basic doubt on restriction digestion - (reply: 2)
1105. Transform staph? - (reply: 2)
1106. help for cDNA synthesis - plus RE problem. (reply: 3)
1107. adjacent restriction sites - (reply: 6)
1108. blunt ligation - (reply: 3)
1109. pENTR TOPO cloning - (reply: 1)
1110. where can i ontain the binary vector pCB301? - (reply: 1)
1082. urgent help regarding wanner method trouble shooting - (reply: 2)
1083. two step digestion - (reply: 6)
1084. Storage of invitrogen top10 cells - (reply: 11)
1085. Self-Ligation of digested vector - (reply: 2)
1086. Help with cloning - (reply: 6)
1087. Ligation issue: Does vector size matter? - (reply: 10)
1088. Help for T-A cloning - (reply: 15)
1089. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1090. Some colonies, but not enough... - (reply: 7)
1091. transformation problem - (reply: 3)
1092. Plasmid sequence - (reply: 1)
1093. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1094. help wih the ligation - (reply: 7)
1095. PCR screening of transformed bacterial colony - (reply: 6)
1096. No colony for oligo cloning - (reply: 9)
1097. cloning - affect on c-terminal (reply: 1)
1098. Growing of TOP10 chemically competent cells - (reply: 4)
1099. Stable Cell Selection with Neomycin - (reply: 4)
1100. lpzzzzz that is so important to my PhD. - (reply: 1)
1101. need guidence for cdna library - screen library (reply: 5)
1102. No product after restriction digestion - (reply: 26)
1103. protein overexpression question. - (reply: 4)
1104. Basic doubt on restriction digestion - (reply: 2)
1105. Transform staph? - (reply: 2)
1106. help for cDNA synthesis - plus RE problem. (reply: 3)
1107. adjacent restriction sites - (reply: 6)
1108. blunt ligation - (reply: 3)
1109. pENTR TOPO cloning - (reply: 1)
1110. where can i ontain the binary vector pCB301? - (reply: 1)