Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1081. Vectors with special MCS - (reply: 5)
1082. Cloning <100 bp fragments into a vector - (reply: 7)
1083. Help! my plasmid size changed after ligation - (reply: 5)
1084. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1085. Restriction digestion of gDNA for phage library - (reply: 5)
1086. how i can get insert back? - (reply: 1)
1087. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1088. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1089. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1090. mammalian expression vector - (reply: 2)
1091. Electroporation doesn't like my plasmid??? - (reply: 4)
1092. low yeild fo RNA after invitro transcription - (reply: 4)
1093. No expresion of transgene - Retroviral vector (reply: 1)
1094. plasmids - plasmids for stable cell line construction (reply: 2)
1095. empty vectors more than the insert - (reply: 6)
1096. Restriction enzyme digestion problem - (reply: 2)
1097. low copy plasmid - how to improve the yield? (reply: 1)
1098. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1099. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
1100. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1101. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1102. Cloning Help!! - It is getting very annoying... (reply: 13)
1103. restriction digestion - (reply: 7)
1104. sequence errors created during cloning - (reply: 6)
1105. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1106. the pyrithiamine - (reply: 5)
1107. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1108. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1109. What is the role of DMSO in yeast transformation - (reply: 1)
1110. ligation problem - (reply: 3)