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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. Help: incompatible RE site subcloning - (reply: 1)
1292. need help cloning cdna of a known protein - (reply: 1)
1293. restriction problem - (reply: 3)
1294. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1295. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1296. Trouble with subcloning - (reply: 6)
1297. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1298. Lb-kan plates life - How long can they be used? (reply: 2)
1299. LR reaction - Cloning into a expression vector (reply: 1)
1300. TOPO-Transformation Problem - (reply: 4)
1301. Checking Plasmid Expression - (reply: 1)
1302. designing primers for genes not sequenced yet - (reply: 2)
1303. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1304. Cloning Problem - (reply: 3)
1305. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1306. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1307. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1308. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1309. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1310. Restriction buffer composition - (reply: 2)
1311. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1312. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1313. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1314. Yet another ligation problem - (reply: 4)
1315. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1316. Promoter Cloning for Luciferase Assay - (reply: 3)
1317. Creating mutants - (reply: 3)
1318. cloning protocol - (reply: 1)
1319. Ligation Time and Temp - (reply: 4)
1320. Miniprep - (reply: 3)