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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. plasmid, BAC, or cosmid? - (reply: 3)
1292. Wierd trouble - dunno where my insert is?? (reply: 3)
1293. Large Molecular weight DNA Extarction - (reply: 7)
1294. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1295. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1296. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1297. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1298. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1299. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1300. Vector sequence included in (PCR) amplified insert - (reply: 2)
1301. COMPETENT CELL PREPARATION - (reply: 6)
1302. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1303. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1304. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1305. T A cloning problems - (reply: 3)
1306. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1307. Maximum recovery time after electroporation - (reply: 2)
1308. pCLBABE puro retriviral vector - (reply: 1)
1309. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1310. Blunt-end cloning problem - (reply: 6)
1311. Supercoiling blocking restriction site? - (reply: 6)
1312. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1313. problem in gene cloning - (reply: 4)
1314. Odd point mutations - (reply: 23)
1315. Plasmid linearization by PCR - (reply: 4)
1316. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1317. can't make the cloning work - (reply: 3)
1318. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1319. cloning help - (reply: 9)
1320. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)