Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1291. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1292. problem in gene cloning - (reply: 4)
1293. Odd point mutations - (reply: 23)
1294. Plasmid linearization by PCR - (reply: 4)
1295. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1296. can't make the cloning work - (reply: 3)
1297. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1298. cloning help - (reply: 9)
1299. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1300. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1301. Ligation trouble continues - subcloning ligation failure (reply: 9)
1302. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1303. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1304. colony PCR - (reply: 8)
1305. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1306. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1307. Truncated sequence - (reply: 1)
1308. gateway cloning - (reply: 3)
1309. Purifying and cloning 69nt product - (reply: 5)
1310. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1311. ligatin issues - (reply: 1)
1312. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1313. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1314. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1315. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1316. A gateway cloning expression vector question - (reply: 1)
1317. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1318. Promoter/gene cloning question - (reply: 7)
1319. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1320. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)