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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1292. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1293. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1294. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1295. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1296. Restriction buffer composition - (reply: 2)
1297. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1298. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1299. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1300. Yet another ligation problem - (reply: 4)
1301. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1302. Promoter Cloning for Luciferase Assay - (reply: 3)
1303. Creating mutants - (reply: 3)
1304. cloning protocol - (reply: 1)
1305. Ligation Time and Temp - (reply: 4)
1306. Miniprep - (reply: 3)
1307. PCR and then ligation - (reply: 7)
1308. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1309. 3 way ligation help needed - (reply: 10)
1310. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1311. Cloning woes - Running out of ideas! - (reply: 7)
1312. What fields of research other than microbiology work with bacteria and - (reply: 1)
1313. How to inoculate bacteria? - (reply: 10)
1314. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1315. need help in my Fse1 restriction ezyme - (reply: 4)
1316. Insert problems with TA cloning - (reply: 1)
1317. Surface expression by tansfection of CHO - (reply: 3)
1318. please help!!! with transformation - (reply: 10)
1319. sequencing primer: T7 or T7promoter? - (reply: 4)
1320. Unstable clone? - (reply: 1)