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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1292. need help in my Fse1 restriction ezyme - (reply: 4)
1293. Insert problems with TA cloning - (reply: 1)
1294. Surface expression by tansfection of CHO - (reply: 3)
1295. please help!!! with transformation - (reply: 10)
1296. sequencing primer: T7 or T7promoter? - (reply: 4)
1297. Unstable clone? - (reply: 1)
1298. Restriction enzymes - (reply: 3)
1299. Problems facing in cloning with pBI121 vector - (reply: 1)
1300. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1301. Is my insert present??? - (reply: 17)
1302. Need help for restriction enzymes - (reply: 5)
1303. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1304. Mini prep - (reply: 4)
1305. adding koazak sequence to primer - (reply: 1)
1306. DNA ligation - (reply: 2)
1307. adding C terminal tag to reverse primer - (reply: 1)
1308. glycerol stocks - (reply: 10)
1309. doubts in sequence submission to NCBI - (reply: 2)
1310. Concentration of DNA for Transformation - (reply: 1)
1311. Restriction digest - (reply: 7)
1312. Help! Double restriction digestion failed.... - (reply: 6)
1313. Kozak sequences.. - (reply: 1)
1314. Sequence management software (for Mac) - (reply: 5)
1315. ligation for 24 hrs in 4*C? - (reply: 3)
1316. Having problem establishing stable cell line...... - (reply: 5)
1317. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)
1318. Contruction of Expression Construct - (reply: 1)
1319. problem with subcloning, please help! - (reply: 8)
1320. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)