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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. Ligation Problems - (reply: 2)
1292. NICKED DNA - (reply: 2)
1293. cDNA clone - (reply: 1)
1294. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
1295. Chosing the right competent cells - (reply: 1)
1296. Checking PCR insert (into pGEMT vector) - (reply: 2)
1297. problem with pGEMT - (reply: 3)
1298. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
1299. Luciferase GL4 siRNA - (reply: 5)
1300. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
1301. Culture Grow well but No Plasmid - (reply: 2)
1302. Insertion Mutation in Insert Sequence - (reply: 2)
1303. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
1304. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
1305. Protein expression with T7 promoter - (reply: 1)
1306. Triple ligation not working - (reply: 1)
1307. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1308. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1309. Problem with Induction (inclusion bodies?) - (reply: 4)
1310. Colony PCR screen positive - insert digestion negative - (reply: 2)
1311. plasmid purification kit for transfection - (reply: 2)
1312. Which pGL4 vector to choose - (reply: 2)
1313. Qiagen midi prepare low yield - (reply: 6)
1314. Help: incompatible RE site subcloning - (reply: 1)
1315. need help cloning cdna of a known protein - (reply: 1)
1316. restriction problem - (reply: 3)
1317. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1318. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1319. Trouble with subcloning - (reply: 6)
1320. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)