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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. Protein expression with T7 promoter - (reply: 1)
1292. Triple ligation not working - (reply: 1)
1293. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
1294. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
1295. Problem with Induction (inclusion bodies?) - (reply: 4)
1296. Colony PCR screen positive - insert digestion negative - (reply: 2)
1297. plasmid purification kit for transfection - (reply: 2)
1298. Which pGL4 vector to choose - (reply: 2)
1299. Qiagen midi prepare low yield - (reply: 6)
1300. Help: incompatible RE site subcloning - (reply: 1)
1301. need help cloning cdna of a known protein - (reply: 1)
1302. restriction problem - (reply: 3)
1303. pGEMT-easy & blue white screening - white colonies don't have insert (reply: 4)
1304. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
1305. Trouble with subcloning - (reply: 6)
1306. Troubleshooting ligation/transformation - strange results when troubleshooting (reply: 1)
1307. Lb-kan plates life - How long can they be used? (reply: 2)
1308. LR reaction - Cloning into a expression vector (reply: 1)
1309. TOPO-Transformation Problem - (reply: 4)
1310. Checking Plasmid Expression - (reply: 1)
1311. designing primers for genes not sequenced yet - (reply: 2)
1312. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1313. Cloning Problem - (reply: 3)
1314. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1315. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1316. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1317. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1318. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1319. Restriction buffer composition - (reply: 2)
1320. same weird weird staph transformation results - any idea what is going on? (reply: 12)