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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. Restriction buffer composition - (reply: 2)
1292. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1293. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1294. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1295. Yet another ligation problem - (reply: 4)
1296. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1297. Promoter Cloning for Luciferase Assay - (reply: 3)
1298. Creating mutants - (reply: 3)
1299. cloning protocol - (reply: 1)
1300. Ligation Time and Temp - (reply: 4)
1301. Miniprep - (reply: 3)
1302. PCR and then ligation - (reply: 7)
1303. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1304. 3 way ligation help needed - (reply: 10)
1305. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1306. Cloning woes - Running out of ideas! - (reply: 7)
1307. What fields of research other than microbiology work with bacteria and - (reply: 1)
1308. How to inoculate bacteria? - (reply: 10)
1309. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1310. need help in my Fse1 restriction ezyme - (reply: 4)
1311. Insert problems with TA cloning - (reply: 1)
1312. Surface expression by tansfection of CHO - (reply: 3)
1313. please help!!! with transformation - (reply: 10)
1314. sequencing primer: T7 or T7promoter? - (reply: 4)
1315. Unstable clone? - (reply: 1)
1316. Restriction enzymes - (reply: 3)
1317. Problems facing in cloning with pBI121 vector - (reply: 1)
1318. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1319. Is my insert present??? - (reply: 17)
1320. Need help for restriction enzymes - (reply: 5)