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Top : New Forum Archives (2009-): : Molecular-Cloning
1291. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1292. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1293. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1294. T A cloning problems - (reply: 3)
1295. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1296. Maximum recovery time after electroporation - (reply: 2)
1297. pCLBABE puro retriviral vector - (reply: 1)
1298. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1299. Blunt-end cloning problem - (reply: 6)
1300. Supercoiling blocking restriction site? - (reply: 6)
1301. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1302. problem in gene cloning - (reply: 4)
1303. Odd point mutations - (reply: 23)
1304. Plasmid linearization by PCR - (reply: 4)
1305. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1306. can't make the cloning work - (reply: 3)
1307. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1308. cloning help - (reply: 9)
1309. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1310. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1311. Ligation trouble continues - subcloning ligation failure (reply: 9)
1312. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1313. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1314. colony PCR - (reply: 8)
1315. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1316. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1317. Truncated sequence - (reply: 1)
1318. gateway cloning - (reply: 3)
1319. Purifying and cloning 69nt product - (reply: 5)
1320. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)