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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Transformation keeps failing - (reply: 2)
242. High background issue - (reply: 5)
243. Pcr primers - (reply: 7)
244. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
245. Gateway vectors - (reply: 3)
246. question on gateway - (reply: 1)
247. Cloning problem still not solved - (reply: 2)
248. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
249. Insert 30nt at C-terminal - (reply: 1)
250. How to store a ligation reaction over the weekend? - (reply: 3)
251. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
252. How homologous do homology arms need to be? - (reply: 3)
253. cloning pEGFP-N1 - (reply: 1)
254. Loss of DNA after restriction digestion cleanup - (reply: 2)
255. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
256. separating mixture of plamids - (reply: 7)
257. Kozak sequence and mammalian expression vector - (reply: 3)
258. GFP vector - (reply: 1)
259. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
260. no colony - (reply: 3)
261. Cloning beginner and clueless! - (reply: 1)
262. Which epitope tag to use for ChIP? - (reply: 1)
263. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
264. Ligation-transformation...Never faced such problem ever - (reply: 6)
265. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
266. Can multiple genes follow only one promoter? - (reply: 1)
267. Restriction Digest Question - (reply: 4)
268. Problem with Large vector & very small insert - (reply: 1)
269. Storage life of plasmid DNA in water - (reply: 5)
270. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)