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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Loss of DNA after restriction digestion cleanup - (reply: 2)
242. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
243. separating mixture of plamids - (reply: 7)
244. Kozak sequence and mammalian expression vector - (reply: 3)
245. GFP vector - (reply: 1)
246. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
247. no colony - (reply: 3)
248. Cloning beginner and clueless! - (reply: 1)
249. Which epitope tag to use for ChIP? - (reply: 1)
250. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
251. Ligation-transformation...Never faced such problem ever - (reply: 6)
252. Can multiple genes follow only one promoter? - (reply: 1)
253. Restriction Digest Question - (reply: 4)
254. Problem with Large vector & very small insert - (reply: 1)
255. Storage life of plasmid DNA in water - (reply: 5)
256. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
257. Site-directed mutagenesis failed - (reply: 20)
258. Insert & vector too dilute to perform ligation - (reply: 3)
259. Cloning Problems - (reply: 2)
260. Suitable templates for in vitro transcription - (reply: 1)
261. Different ways to ensure the successful cloning? - (reply: 2)
262. Plasmid DNA digestion problem - (reply: 3)
263. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
264. Single codon insert - (reply: 3)
265. cloning- ligation - (reply: 3)
266. Sticky and blunt ligation issue - (reply: 6)
267. Handling DEPC under nitrogen/argon - (reply: 3)
268. Incubation period after heat shock at transformation--reason? - (reply: 3)
269. PCR product sequencing - (reply: 3)
270. What is the optimal time for transfection efficiency measuring? - (reply: 1)