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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Cloning beginner and clueless! - (reply: 1)
242. Which epitope tag to use for ChIP? - (reply: 1)
243. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
244. Ligation-transformation...Never faced such problem ever - (reply: 6)
245. Can multiple genes follow only one promoter? - (reply: 1)
246. Restriction Digest Question - (reply: 4)
247. Problem with Large vector & very small insert - (reply: 1)
248. Storage life of plasmid DNA in water - (reply: 5)
249. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
250. Site-directed mutagenesis failed - (reply: 20)
251. Insert & vector too dilute to perform ligation - (reply: 3)
252. Cloning Problems - (reply: 2)
253. Suitable templates for in vitro transcription - (reply: 1)
254. Different ways to ensure the successful cloning? - (reply: 2)
255. Plasmid DNA digestion problem - (reply: 3)
256. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
257. Single codon insert - (reply: 3)
258. cloning- ligation - (reply: 3)
259. Sticky and blunt ligation issue - (reply: 6)
260. Handling DEPC under nitrogen/argon - (reply: 3)
261. Incubation period after heat shock at transformation--reason? - (reply: 3)
262. PCR product sequencing - (reply: 3)
263. What is the optimal time for transfection efficiency measuring? - (reply: 1)
264. i couldn't get the band of target gene after ligation - (reply: 9)
265. Minimum homology bases needed for homologous recombination - (reply: 1)
266. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
267. Expansion of commercial competent bacterial cell lines? - (reply: 2)
268. Manipulation of target DNA fragments - (reply: 1)
269. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
270. Appearance of a salty plasmid preparation - (reply: 1)