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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Site-directed mutagenesis failed - (reply: 20)
242. Insert & vector too dilute to perform ligation - (reply: 3)
243. Cloning Problems - (reply: 2)
244. Suitable templates for in vitro transcription - (reply: 1)
245. Different ways to ensure the successful cloning? - (reply: 2)
246. Plasmid DNA digestion problem - (reply: 3)
247. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
248. Single codon insert - (reply: 3)
249. cloning- ligation - (reply: 3)
250. Sticky and blunt ligation issue - (reply: 6)
251. Handling DEPC under nitrogen/argon - (reply: 3)
252. Incubation period after heat shock at transformation--reason? - (reply: 3)
253. PCR product sequencing - (reply: 3)
254. What is the optimal time for transfection efficiency measuring? - (reply: 1)
255. i couldn't get the band of target gene after ligation - (reply: 9)
256. Minimum homology bases needed for homologous recombination - (reply: 1)
257. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
258. Expansion of commercial competent bacterial cell lines? - (reply: 2)
259. Manipulation of target DNA fragments - (reply: 1)
260. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
261. Appearance of a salty plasmid preparation - (reply: 1)
262. origami bacteria transformation - (reply: 5)
263. Smallest mRNA length that can be translated - (reply: 3)
264. wrong insert? right insert?? - (reply: 2)
265. Help~ Incorrect constructs in cloning - (reply: 3)
266. Problems with ligation and transformation - (reply: 2)
267. Transformation of Restricted plasmid - (reply: 3)
268. Scrambled sequence? - (reply: 3)
269. Why is my insert missing from my plasmid? - (reply: 4)
270. PCR Profile for ligation - (reply: 3)