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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Whole plasmid amplification by PCR - (reply: 2)
242. colony PCR after transformation - (reply: 1)
243. For promoter assay, which vector I should use? - (reply: 5)
244. Sequencing of the cloned plasmid - (reply: 5)
245. 4 way cloning into TA Vector - (reply: 10)
246. I get band double the size what does it mean - (reply: 3)
247. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
248. Neeed Help , cloning Confirmation ?! - (reply: 5)
249. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
250. Can I quantify restricted plasmid and oligos? - (reply: 3)
251. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
252. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
253. TOPO TA ligation problems - (reply: 6)
254. Luciferase assay problem - (reply: 2)
255. Double digestion with BamHI and BglII - (reply: 4)
256. nicked linear DNA - (reply: 5)
257. Selection of E.coli competent cells - (reply: 2)
258. Double strand cdna synthysis - (reply: 4)
259. mutagenic primers with very high GC content. - (reply: 3)
260. TA Cloning of ds cDNA - (reply: 6)
261. Promoter distance from ATG - (reply: 3)
262. Measuring Fluorescene of RFP - (reply: 7)
263. Smallest possible insert size for Ligation reaction - (reply: 2)
264. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
265. Extraction of DNA from termite gut flagellates - (reply: 7)
266. Enzymes and buffers from different companies - (reply: 6)
267. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
268. faint band after plasmid extraction - (reply: 7)
269. Adaptor ligation - (reply: 9)
270. self ligation - (reply: 1)