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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Which epitope tag to use for ChIP? - (reply: 1)
242. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
243. Ligation-transformation...Never faced such problem ever - (reply: 6)
244. Can multiple genes follow only one promoter? - (reply: 1)
245. Restriction Digest Question - (reply: 4)
246. Problem with Large vector & very small insert - (reply: 1)
247. Storage life of plasmid DNA in water - (reply: 5)
248. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
249. Site-directed mutagenesis failed - (reply: 20)
250. Insert & vector too dilute to perform ligation - (reply: 3)
251. Cloning Problems - (reply: 2)
252. Suitable templates for in vitro transcription - (reply: 1)
253. Different ways to ensure the successful cloning? - (reply: 2)
254. Plasmid DNA digestion problem - (reply: 3)
255. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
256. Single codon insert - (reply: 3)
257. cloning- ligation - (reply: 3)
258. Sticky and blunt ligation issue - (reply: 6)
259. Handling DEPC under nitrogen/argon - (reply: 3)
260. Incubation period after heat shock at transformation--reason? - (reply: 3)
261. PCR product sequencing - (reply: 3)
262. What is the optimal time for transfection efficiency measuring? - (reply: 1)
263. i couldn't get the band of target gene after ligation - (reply: 9)
264. Minimum homology bases needed for homologous recombination - (reply: 1)
265. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
266. Expansion of commercial competent bacterial cell lines? - (reply: 2)
267. Manipulation of target DNA fragments - (reply: 1)
268. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
269. Appearance of a salty plasmid preparation - (reply: 1)
270. origami bacteria transformation - (reply: 5)