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Top : New Forum Archives (2009-): : Molecular-Cloning
241. no insert issue - (reply: 9)
242. Considerations on the design of a transgene - (reply: 1)
243. How to obtain Vectors used in articles - (reply: 5)
244. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
245. How efficient is the 2A sequence - (reply: 2)
246. Transformation keeps failing - (reply: 2)
247. High background issue - (reply: 5)
248. Pcr primers - (reply: 7)
249. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
250. Gateway vectors - (reply: 3)
251. question on gateway - (reply: 1)
252. Cloning problem still not solved - (reply: 2)
253. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
254. Insert 30nt at C-terminal - (reply: 1)
255. How to store a ligation reaction over the weekend? - (reply: 3)
256. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
257. How homologous do homology arms need to be? - (reply: 3)
258. cloning pEGFP-N1 - (reply: 1)
259. Loss of DNA after restriction digestion cleanup - (reply: 2)
260. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
261. separating mixture of plamids - (reply: 7)
262. Kozak sequence and mammalian expression vector - (reply: 3)
263. GFP vector - (reply: 1)
264. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
265. no colony - (reply: 3)
266. Cloning beginner and clueless! - (reply: 1)
267. Which epitope tag to use for ChIP? - (reply: 1)
268. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
269. Ligation-transformation...Never faced such problem ever - (reply: 6)
270. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)