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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Pcr primers - (reply: 7)
242. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
243. Gateway vectors - (reply: 3)
244. question on gateway - (reply: 1)
245. Cloning problem still not solved - (reply: 2)
246. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
247. Insert 30nt at C-terminal - (reply: 1)
248. How to store a ligation reaction over the weekend? - (reply: 3)
249. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
250. How homologous do homology arms need to be? - (reply: 3)
251. cloning pEGFP-N1 - (reply: 1)
252. Loss of DNA after restriction digestion cleanup - (reply: 2)
253. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
254. separating mixture of plamids - (reply: 7)
255. Kozak sequence and mammalian expression vector - (reply: 3)
256. GFP vector - (reply: 1)
257. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
258. no colony - (reply: 3)
259. Cloning beginner and clueless! - (reply: 1)
260. Which epitope tag to use for ChIP? - (reply: 1)
261. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
262. Ligation-transformation...Never faced such problem ever - (reply: 6)
263. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
264. Can multiple genes follow only one promoter? - (reply: 1)
265. Restriction Digest Question - (reply: 4)
266. Problem with Large vector & very small insert - (reply: 1)
267. Storage life of plasmid DNA in water - (reply: 5)
268. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
269. Site-directed mutagenesis failed - (reply: 20)
270. Insert & vector too dilute to perform ligation - (reply: 3)