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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
242. Insert 30nt at C-terminal - (reply: 1)
243. How to store a ligation reaction over the weekend? - (reply: 3)
244. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
245. How homologous do homology arms need to be? - (reply: 3)
246. cloning pEGFP-N1 - (reply: 1)
247. Loss of DNA after restriction digestion cleanup - (reply: 2)
248. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
249. separating mixture of plamids - (reply: 7)
250. Kozak sequence and mammalian expression vector - (reply: 3)
251. GFP vector - (reply: 1)
252. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
253. no colony - (reply: 3)
254. Cloning beginner and clueless! - (reply: 1)
255. Which epitope tag to use for ChIP? - (reply: 1)
256. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
257. Ligation-transformation...Never faced such problem ever - (reply: 6)
258. Can multiple genes follow only one promoter? - (reply: 1)
259. Restriction Digest Question - (reply: 4)
260. Problem with Large vector & very small insert - (reply: 1)
261. Storage life of plasmid DNA in water - (reply: 5)
262. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
263. Site-directed mutagenesis failed - (reply: 20)
264. Insert & vector too dilute to perform ligation - (reply: 3)
265. Cloning Problems - (reply: 2)
266. Suitable templates for in vitro transcription - (reply: 1)
267. Different ways to ensure the successful cloning? - (reply: 2)
268. Plasmid DNA digestion problem - (reply: 3)
269. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
270. Single codon insert - (reply: 3)