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Top : New Forum Archives (2009-): : Molecular-Cloning
241. How efficient is the 2A sequence - (reply: 2)
242. Transformation keeps failing - (reply: 2)
243. High background issue - (reply: 5)
244. Pcr primers - (reply: 7)
245. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
246. Gateway vectors - (reply: 3)
247. question on gateway - (reply: 1)
248. Cloning problem still not solved - (reply: 2)
249. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
250. Insert 30nt at C-terminal - (reply: 1)
251. How to store a ligation reaction over the weekend? - (reply: 3)
252. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
253. How homologous do homology arms need to be? - (reply: 3)
254. cloning pEGFP-N1 - (reply: 1)
255. Loss of DNA after restriction digestion cleanup - (reply: 2)
256. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
257. separating mixture of plamids - (reply: 7)
258. Kozak sequence and mammalian expression vector - (reply: 3)
259. GFP vector - (reply: 1)
260. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
261. no colony - (reply: 3)
262. Cloning beginner and clueless! - (reply: 1)
263. Which epitope tag to use for ChIP? - (reply: 1)
264. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
265. Ligation-transformation...Never faced such problem ever - (reply: 6)
266. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
267. Can multiple genes follow only one promoter? - (reply: 1)
268. Restriction Digest Question - (reply: 4)
269. Problem with Large vector & very small insert - (reply: 1)
270. Storage life of plasmid DNA in water - (reply: 5)