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Top : New Forum Archives (2009-): : Molecular-Cloning
241. separating mixture of plamids - (reply: 7)
242. Kozak sequence and mammalian expression vector - (reply: 3)
243. GFP vector - (reply: 1)
244. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
245. no colony - (reply: 3)
246. Cloning beginner and clueless! - (reply: 1)
247. Which epitope tag to use for ChIP? - (reply: 1)
248. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
249. Ligation-transformation...Never faced such problem ever - (reply: 6)
250. Can multiple genes follow only one promoter? - (reply: 1)
251. Restriction Digest Question - (reply: 4)
252. Problem with Large vector & very small insert - (reply: 1)
253. Storage life of plasmid DNA in water - (reply: 5)
254. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
255. Site-directed mutagenesis failed - (reply: 20)
256. Insert & vector too dilute to perform ligation - (reply: 3)
257. Cloning Problems - (reply: 2)
258. Suitable templates for in vitro transcription - (reply: 1)
259. Different ways to ensure the successful cloning? - (reply: 2)
260. Plasmid DNA digestion problem - (reply: 3)
261. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
262. Single codon insert - (reply: 3)
263. cloning- ligation - (reply: 3)
264. Sticky and blunt ligation issue - (reply: 6)
265. Handling DEPC under nitrogen/argon - (reply: 3)
266. Incubation period after heat shock at transformation--reason? - (reply: 3)
267. PCR product sequencing - (reply: 3)
268. What is the optimal time for transfection efficiency measuring? - (reply: 1)
269. i couldn't get the band of target gene after ligation - (reply: 9)
270. Minimum homology bases needed for homologous recombination - (reply: 1)