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Top : New Forum Archives (2009-): : Molecular-Cloning
241. New ordered Restriction Enzymes not working, need help! - (reply: 2)
242. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
243. Transfection but no expression - (reply: 4)
244. Change nucleotide - (reply: 1)
245. Colonies grown without plasmid - (reply: 7)
246. unwanted band - (reply: 5)
247. Colony PCR positive and Digestion negative????? - (reply: 11)
248. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
249. Plasmid on blotting paper - (reply: 1)
250. Dimerization of PCR product - (reply: 4)
251. How to generate Lentivirus? - (reply: 4)
252. Do genes always need to be cloned in the "right direction"? - (reply: 3)
253. Gel purification (DNA) - (reply: 1)
254. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
255. Cloning and expression problem - (reply: 5)
256. How do silicates adhere nucleic acid? - (reply: 1)
257. Ligation of PCR fragments - (reply: 11)
258. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
259. What plasmid sizes do you work with for SDM? - (reply: 1)
260. cloning help; high salt level in vector - (reply: 4)
261. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
262. problem with gateway cloning: missing part of insert - (reply: 2)
263. T7 and M13 primers two band amplification - (reply: 3)
264. Type of polymerases compatible for TA cloning - (reply: 7)
265. Running undigested plasmids on gel - (reply: 2)
266. Molecular Cloning Sambrook - (reply: 1)
267. What is the membrane made of in DNA purification kits - (reply: 3)
268. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
269. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
270. Removing Introns from a DNA sequence - (reply: 1)