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Top : New Forum Archives (2009-): : Molecular-Cloning
241. no colonies, ever! - (reply: 5)
242. fluorescent tag on membrane protein - (reply: 2)
243. How can addgene sell plasmids with patented technology - (reply: 2)
244. no insert issue - (reply: 9)
245. Considerations on the design of a transgene - (reply: 1)
246. How to obtain Vectors used in articles - (reply: 5)
247. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
248. How efficient is the 2A sequence - (reply: 2)
249. Transformation keeps failing - (reply: 2)
250. High background issue - (reply: 5)
251. Pcr primers - (reply: 7)
252. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
253. Gateway vectors - (reply: 3)
254. question on gateway - (reply: 1)
255. Cloning problem still not solved - (reply: 2)
256. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
257. Insert 30nt at C-terminal - (reply: 1)
258. How to store a ligation reaction over the weekend? - (reply: 3)
259. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
260. How homologous do homology arms need to be? - (reply: 3)
261. cloning pEGFP-N1 - (reply: 1)
262. Loss of DNA after restriction digestion cleanup - (reply: 2)
263. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
264. separating mixture of plamids - (reply: 7)
265. Kozak sequence and mammalian expression vector - (reply: 3)
266. GFP vector - (reply: 1)
267. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
268. no colony - (reply: 3)
269. Cloning beginner and clueless! - (reply: 1)
270. Which epitope tag to use for ChIP? - (reply: 1)