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Top : New Forum Archives (2009-): : Molecular-Cloning
241. GFP vector - (reply: 1)
242. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
243. no colony - (reply: 3)
244. Cloning beginner and clueless! - (reply: 1)
245. Which epitope tag to use for ChIP? - (reply: 1)
246. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
247. Ligation-transformation...Never faced such problem ever - (reply: 6)
248. Can multiple genes follow only one promoter? - (reply: 1)
249. Restriction Digest Question - (reply: 4)
250. Problem with Large vector & very small insert - (reply: 1)
251. Storage life of plasmid DNA in water - (reply: 5)
252. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
253. Site-directed mutagenesis failed - (reply: 20)
254. Insert & vector too dilute to perform ligation - (reply: 3)
255. Cloning Problems - (reply: 2)
256. Suitable templates for in vitro transcription - (reply: 1)
257. Different ways to ensure the successful cloning? - (reply: 2)
258. Plasmid DNA digestion problem - (reply: 3)
259. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
260. Single codon insert - (reply: 3)
261. cloning- ligation - (reply: 3)
262. Sticky and blunt ligation issue - (reply: 6)
263. Handling DEPC under nitrogen/argon - (reply: 3)
264. Incubation period after heat shock at transformation--reason? - (reply: 3)
265. PCR product sequencing - (reply: 3)
266. What is the optimal time for transfection efficiency measuring? - (reply: 1)
267. i couldn't get the band of target gene after ligation - (reply: 9)
268. Minimum homology bases needed for homologous recombination - (reply: 1)
269. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
270. Expansion of commercial competent bacterial cell lines? - (reply: 2)