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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Appearance of a salty plasmid preparation - (reply: 1)
242. origami bacteria transformation - (reply: 5)
243. Smallest mRNA length that can be translated - (reply: 3)
244. wrong insert? right insert?? - (reply: 2)
245. Help~ Incorrect constructs in cloning - (reply: 3)
246. Problems with ligation and transformation - (reply: 2)
247. Transformation of Restricted plasmid - (reply: 3)
248. Scrambled sequence? - (reply: 3)
249. Why is my insert missing from my plasmid? - (reply: 4)
250. PCR Profile for ligation - (reply: 3)
251. Blunt end is ligating to sticky end? - (reply: 2)
252. SNP in the promoter region - (reply: 1)
253. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
254. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
255. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
256. New ordered Restriction Enzymes not working, need help! - (reply: 2)
257. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
258. Transfection but no expression - (reply: 4)
259. Change nucleotide - (reply: 1)
260. Colonies grown without plasmid - (reply: 7)
261. unwanted band - (reply: 5)
262. Colony PCR positive and Digestion negative????? - (reply: 11)
263. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
264. Plasmid on blotting paper - (reply: 1)
265. Dimerization of PCR product - (reply: 4)
266. How to generate Lentivirus? - (reply: 4)
267. Do genes always need to be cloned in the "right direction"? - (reply: 3)
268. Gel purification (DNA) - (reply: 1)
269. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
270. Cloning and expression problem - (reply: 5)