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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Running undigested plasmids on gel - (reply: 2)
242. Molecular Cloning Sambrook - (reply: 1)
243. What is the membrane made of in DNA purification kits - (reply: 3)
244. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
245. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
246. Removing Introns from a DNA sequence - (reply: 1)
247. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
248. without RE site in PCR product - (reply: 5)
249. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
250. excision of fragment during cloning - (reply: 4)
251. Unusual cloning issue... anyone else?? - (reply: 4)
252. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
253. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
254. How to avoid RNA contamination in DNA samples:? - (reply: 1)
255. Wavy Lines in Gel - (reply: 2)
256. Question Relating to LacZ Disruption - (reply: 4)
257. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
258. Site directed mutagenesis - (reply: 5)
259. PCR insert - in frame? - (reply: 2)
260. Whole plasmid amplification by PCR - (reply: 2)
261. colony PCR after transformation - (reply: 1)
262. For promoter assay, which vector I should use? - (reply: 5)
263. Sequencing of the cloned plasmid - (reply: 5)
264. 4 way cloning into TA Vector - (reply: 10)
265. I get band double the size what does it mean - (reply: 3)
266. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
267. Neeed Help , cloning Confirmation ?! - (reply: 5)
268. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
269. Can I quantify restricted plasmid and oligos? - (reply: 3)
270. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)