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Top : New Forum Archives (2009-): : Molecular-Cloning
241. fluorescent tag on membrane protein - (reply: 2)
242. How can addgene sell plasmids with patented technology - (reply: 2)
243. no insert issue - (reply: 9)
244. Considerations on the design of a transgene - (reply: 1)
245. How to obtain Vectors used in articles - (reply: 5)
246. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
247. How efficient is the 2A sequence - (reply: 2)
248. Transformation keeps failing - (reply: 2)
249. High background issue - (reply: 5)
250. Pcr primers - (reply: 7)
251. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
252. Gateway vectors - (reply: 3)
253. question on gateway - (reply: 1)
254. Cloning problem still not solved - (reply: 2)
255. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
256. Insert 30nt at C-terminal - (reply: 1)
257. How to store a ligation reaction over the weekend? - (reply: 3)
258. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
259. How homologous do homology arms need to be? - (reply: 3)
260. cloning pEGFP-N1 - (reply: 1)
261. Loss of DNA after restriction digestion cleanup - (reply: 2)
262. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
263. separating mixture of plamids - (reply: 7)
264. Kozak sequence and mammalian expression vector - (reply: 3)
265. GFP vector - (reply: 1)
266. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
267. no colony - (reply: 3)
268. Cloning beginner and clueless! - (reply: 1)
269. Which epitope tag to use for ChIP? - (reply: 1)
270. who else can help me check primer. im a little bit less confident :(( - (reply: 9)