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Top : New Forum Archives (2009-): : Molecular-Cloning
241. How can addgene sell plasmids with patented technology - (reply: 2)
242. no insert issue - (reply: 9)
243. Considerations on the design of a transgene - (reply: 1)
244. How to obtain Vectors used in articles - (reply: 5)
245. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
246. How efficient is the 2A sequence - (reply: 2)
247. Transformation keeps failing - (reply: 2)
248. High background issue - (reply: 5)
249. Pcr primers - (reply: 7)
250. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
251. Gateway vectors - (reply: 3)
252. question on gateway - (reply: 1)
253. Cloning problem still not solved - (reply: 2)
254. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
255. Insert 30nt at C-terminal - (reply: 1)
256. How to store a ligation reaction over the weekend? - (reply: 3)
257. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
258. How homologous do homology arms need to be? - (reply: 3)
259. cloning pEGFP-N1 - (reply: 1)
260. Loss of DNA after restriction digestion cleanup - (reply: 2)
261. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
262. separating mixture of plamids - (reply: 7)
263. Kozak sequence and mammalian expression vector - (reply: 3)
264. GFP vector - (reply: 1)
265. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
266. no colony - (reply: 3)
267. Cloning beginner and clueless! - (reply: 1)
268. Which epitope tag to use for ChIP? - (reply: 1)
269. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
270. Ligation-transformation...Never faced such problem ever - (reply: 6)