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Top : New Forum Archives (2009-): : Molecular-Cloning
241. wrong insert? right insert?? - (reply: 2)
242. Help~ Incorrect constructs in cloning - (reply: 3)
243. Problems with ligation and transformation - (reply: 2)
244. Transformation of Restricted plasmid - (reply: 3)
245. Scrambled sequence? - (reply: 3)
246. Why is my insert missing from my plasmid? - (reply: 4)
247. PCR Profile for ligation - (reply: 3)
248. Blunt end is ligating to sticky end? - (reply: 2)
249. SNP in the promoter region - (reply: 1)
250. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
251. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
252. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
253. New ordered Restriction Enzymes not working, need help! - (reply: 2)
254. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
255. Transfection but no expression - (reply: 4)
256. Change nucleotide - (reply: 1)
257. Colonies grown without plasmid - (reply: 7)
258. unwanted band - (reply: 5)
259. Colony PCR positive and Digestion negative????? - (reply: 11)
260. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
261. Plasmid on blotting paper - (reply: 1)
262. Dimerization of PCR product - (reply: 4)
263. How to generate Lentivirus? - (reply: 4)
264. Do genes always need to be cloned in the "right direction"? - (reply: 3)
265. Gel purification (DNA) - (reply: 1)
266. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
267. Cloning and expression problem - (reply: 5)
268. How do silicates adhere nucleic acid? - (reply: 1)
269. Ligation of PCR fragments - (reply: 11)
270. No Colonies after Electroporation of P.aeruginosa - (reply: 6)