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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
242. Gateway vectors - (reply: 3)
243. question on gateway - (reply: 1)
244. Cloning problem still not solved - (reply: 2)
245. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
246. Insert 30nt at C-terminal - (reply: 1)
247. How to store a ligation reaction over the weekend? - (reply: 3)
248. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
249. How homologous do homology arms need to be? - (reply: 3)
250. cloning pEGFP-N1 - (reply: 1)
251. Loss of DNA after restriction digestion cleanup - (reply: 2)
252. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
253. separating mixture of plamids - (reply: 7)
254. Kozak sequence and mammalian expression vector - (reply: 3)
255. GFP vector - (reply: 1)
256. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
257. no colony - (reply: 3)
258. Cloning beginner and clueless! - (reply: 1)
259. Which epitope tag to use for ChIP? - (reply: 1)
260. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
261. Ligation-transformation...Never faced such problem ever - (reply: 6)
262. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 1)
263. Can multiple genes follow only one promoter? - (reply: 1)
264. Restriction Digest Question - (reply: 4)
265. Problem with Large vector & very small insert - (reply: 1)
266. Storage life of plasmid DNA in water - (reply: 5)
267. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
268. Site-directed mutagenesis failed - (reply: 20)
269. Insert & vector too dilute to perform ligation - (reply: 3)
270. Cloning Problems - (reply: 2)