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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
242. Appearance of a salty plasmid preparation - (reply: 1)
243. origami bacteria transformation - (reply: 5)
244. Smallest mRNA length that can be translated - (reply: 3)
245. wrong insert? right insert?? - (reply: 2)
246. Help~ Incorrect constructs in cloning - (reply: 3)
247. Problems with ligation and transformation - (reply: 2)
248. Transformation of Restricted plasmid - (reply: 3)
249. Scrambled sequence? - (reply: 3)
250. Why is my insert missing from my plasmid? - (reply: 4)
251. PCR Profile for ligation - (reply: 3)
252. Blunt end is ligating to sticky end? - (reply: 2)
253. SNP in the promoter region - (reply: 1)
254. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
255. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
256. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
257. New ordered Restriction Enzymes not working, need help! - (reply: 2)
258. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
259. Transfection but no expression - (reply: 4)
260. Change nucleotide - (reply: 1)
261. Colonies grown without plasmid - (reply: 7)
262. unwanted band - (reply: 5)
263. Colony PCR positive and Digestion negative????? - (reply: 11)
264. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
265. Plasmid on blotting paper - (reply: 1)
266. Dimerization of PCR product - (reply: 4)
267. How to generate Lentivirus? - (reply: 4)
268. Do genes always need to be cloned in the "right direction"? - (reply: 3)
269. Gel purification (DNA) - (reply: 1)
270. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)