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Top : New Forum Archives (2009-): : Molecular-Cloning
241. How homologous do homology arms need to be? - (reply: 3)
242. cloning pEGFP-N1 - (reply: 1)
243. Loss of DNA after restriction digestion cleanup - (reply: 2)
244. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
245. separating mixture of plamids - (reply: 7)
246. Kozak sequence and mammalian expression vector - (reply: 3)
247. GFP vector - (reply: 1)
248. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
249. no colony - (reply: 3)
250. Cloning beginner and clueless! - (reply: 1)
251. Which epitope tag to use for ChIP? - (reply: 1)
252. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
253. Ligation-transformation...Never faced such problem ever - (reply: 6)
254. Can multiple genes follow only one promoter? - (reply: 1)
255. Restriction Digest Question - (reply: 4)
256. Problem with Large vector & very small insert - (reply: 1)
257. Storage life of plasmid DNA in water - (reply: 5)
258. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
259. Site-directed mutagenesis failed - (reply: 20)
260. Insert & vector too dilute to perform ligation - (reply: 3)
261. Cloning Problems - (reply: 2)
262. Suitable templates for in vitro transcription - (reply: 1)
263. Different ways to ensure the successful cloning? - (reply: 2)
264. Plasmid DNA digestion problem - (reply: 3)
265. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
266. Single codon insert - (reply: 3)
267. cloning- ligation - (reply: 3)
268. Sticky and blunt ligation issue - (reply: 6)
269. Handling DEPC under nitrogen/argon - (reply: 3)
270. Incubation period after heat shock at transformation--reason? - (reply: 3)