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Top : New Forum Archives (2009-): : Molecular-Cloning
241. Problems with crossover PCR - (reply: 2)
242. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
243. Two genes, same MCS - (reply: 1)
244. Including ATG in cloning? - (reply: 1)
245. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
246. Low copy vector for library generation - (reply: 2)
247. Can't find a recombinant after ligating - (reply: 4)
248. problems with plasmid miniprep - (reply: 8)
249. gene cloning - (reply: 1)
250. Is ligation really necessary? - (reply: 6)
251. problem with ligation of linker/adaptor - (reply: 2)
252. GFP -weak signal -why? - (reply: 1)
253. Ligation troubleshooting! Please help! - (reply: 5)
254. Truncated gene Cloning - (reply: 1)
255. Screening for ligation and transformation result - (reply: 2)
256. Problem with 3 way ligation - (reply: 2)
257. Query regarding primers for quick change mutagenesis - (reply: 3)
258. What is NEB's CutSmart Buffer? - (reply: 5)
259. Enzyme cutting: low efficiency - (reply: 5)
260. Issue with Xba1 and Dam methylation - (reply: 7)
261. Restriction site rearrangement - (reply: 1)
262. Mutation in cloning - (reply: 6)
263. Must the terminator be in frame? - (reply: 1)
264. sequencing problems - (reply: 2)
265. Understand the basics of molecular cloning - (reply: 6)
266. Maintainence of transformed cells - (reply: 5)
267. Restriction digest band shift? - (reply: 1)
268. problem in cloning PCR primer design with restriction site - (reply: 4)
269. quick change mutagenesis...... - (reply: 1)
270. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)