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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Problem shrna cloning, bad sequencing results - (reply: 1)
152. Lentivirus copy number - (reply: 7)
153. Genomic -Lactamase? Blue White screening not working - (reply: 1)
154. direction of the expression units in multi-gene vectors - (reply: 1)
155. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
156. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
157. Measuring pcr fragments in a gel - (reply: 1)
158. gel electrophoresis - (reply: 4)
159. three questions about CRISPR knowout - (reply: 2)
160. Help needed for Ligation/Transformation - (reply: 1)
161. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
162. ClaI restriction enzyme efficency - (reply: 1)
163. Blunt end cloning - (reply: 12)
164. sequantial digest - (reply: 25)
165. Temperature sensitivity of Plasmids - (reply: 1)
166. DMSO in restriction digest - (reply: 1)
167. Molecular cloning - (reply: 5)
168. Nuclease free water for cloning - (reply: 8)
169. Directional cloning 2bps overhang - (reply: 3)
170. I need help designing homology arms for CRISPR - (reply: 3)
171. Partial digest XhoI NotI NEB - (reply: 6)
172. removing part from plasmid - (reply: 18)
173. Start codon after promoter - (reply: 1)
174. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
175. Purpose of PolyA in cloning - (reply: 1)
176. Ligation failure? I ran some on a gel - (reply: 6)
177. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
178. Cloning into pVIVO2 vector - (reply: 8)
179. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
180. Cloning worked, but the expression vector is weird - (reply: 6)