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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Plasmid DNA digestion problem - (reply: 3)
152. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
153. Single codon insert - (reply: 3)
154. cloning- ligation - (reply: 3)
155. Sticky and blunt ligation issue - (reply: 6)
156. Handling DEPC under nitrogen/argon - (reply: 3)
157. Incubation period after heat shock at transformation--reason? - (reply: 3)
158. PCR product sequencing - (reply: 3)
159. What is the optimal time for transfection efficiency measuring? - (reply: 1)
160. i couldn't get the band of target gene after ligation - (reply: 9)
161. Minimum homology bases needed for homologous recombination - (reply: 1)
162. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
163. Expansion of commercial competent bacterial cell lines? - (reply: 2)
164. Manipulation of target DNA fragments - (reply: 1)
165. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
166. Appearance of a salty plasmid preparation - (reply: 1)
167. origami bacteria transformation - (reply: 5)
168. Smallest mRNA length that can be translated - (reply: 3)
169. wrong insert? right insert?? - (reply: 2)
170. Help~ Incorrect constructs in cloning - (reply: 3)
171. Problems with ligation and transformation - (reply: 2)
172. Transformation of Restricted plasmid - (reply: 3)
173. Scrambled sequence? - (reply: 3)
174. Why is my insert missing from my plasmid? - (reply: 4)
175. PCR Profile for ligation - (reply: 3)
176. Blunt end is ligating to sticky end? - (reply: 2)
177. SNP in the promoter region - (reply: 1)
178. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
179. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
180. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)