Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
151. restriction enzymes/sites to avoid - (reply: 4)
152. Using pBABE plasmids for regular transfection - (reply: 1)
153. Ubiquitin promoter cloning - (reply: 1)
154. Cloning two genes in the same plasmid with one promotor - (reply: 4)
155. Sending a TOPO-construct for sequencing? - (reply: 1)
156. Is this counter-productive cloning or not? - (reply: 8)
157. Colony screening - (reply: 1)
158. Failed cloning for 1month - TOPO - (reply: 7)
159. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
160. Which method for DNA purification b4 cloning - (reply: 4)
161. Purification for TOPO - (reply: 1)
162. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
163. Failed TOPO cloning - what happened? - (reply: 9)
164. TOPO cloning after Phusion-PCR - (reply: 1)
165. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
166. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
167. Deleting 7Mbp chromosomal region - (reply: 3)
168. Electrophoresis samples and volumes - (reply: 1)
169. Gene cloning with unknown sequence - (reply: 6)
170. Checking for efficieny competent cells - (reply: 1)
171. Troubles with Fusion PCR - (reply: 1)
172. Does this look alright? - (reply: 3)
173. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
174. Cloning pDsRed-Monomer-N1 - (reply: 1)
175. forgot to heat shock - (reply: 6)
176. Ligation left for weekend at room temp - (reply: 4)
177. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
178. Problem in plasmid digestion after transformation - (reply: 4)
179. Lac Promoter activity in P. aeruginosa - (reply: 1)
180. Resctriction analysis - (reply: 5)