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Top : New Forum Archives (2009-): : Molecular-Cloning
151. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
152. Measuring pcr fragments in a gel - (reply: 1)
153. gel electrophoresis - (reply: 4)
154. three questions about CRISPR knowout - (reply: 2)
155. Help needed for Ligation/Transformation - (reply: 1)
156. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
157. ClaI restriction enzyme efficency - (reply: 1)
158. Blunt end cloning - (reply: 12)
159. sequantial digest - (reply: 25)
160. Temperature sensitivity of Plasmids - (reply: 1)
161. DMSO in restriction digest - (reply: 1)
162. Molecular cloning - (reply: 5)
163. Nuclease free water for cloning - (reply: 8)
164. Directional cloning 2bps overhang - (reply: 3)
165. I need help designing homology arms for CRISPR - (reply: 3)
166. Partial digest XhoI NotI NEB - (reply: 6)
167. removing part from plasmid - (reply: 18)
168. Start codon after promoter - (reply: 1)
169. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
170. Purpose of PolyA in cloning - (reply: 1)
171. Ligation failure? I ran some on a gel - (reply: 6)
172. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
173. Cloning into pVIVO2 vector - (reply: 8)
174. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
175. Cloning worked, but the expression vector is weird - (reply: 6)
176. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
177. site directed mutagenesis - (reply: 3)
178. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
179. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
180. restriction enzymes/sites to avoid - (reply: 4)