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Top : New Forum Archives (2009-): : Molecular-Cloning
151. cloning- ligation - (reply: 3)
152. Sticky and blunt ligation issue - (reply: 6)
153. Handling DEPC under nitrogen/argon - (reply: 3)
154. Incubation period after heat shock at transformation--reason? - (reply: 3)
155. PCR product sequencing - (reply: 3)
156. What is the optimal time for transfection efficiency measuring? - (reply: 1)
157. i couldn't get the band of target gene after ligation - (reply: 9)
158. Minimum homology bases needed for homologous recombination - (reply: 1)
159. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
160. Expansion of commercial competent bacterial cell lines? - (reply: 2)
161. Manipulation of target DNA fragments - (reply: 1)
162. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
163. Appearance of a salty plasmid preparation - (reply: 1)
164. origami bacteria transformation - (reply: 5)
165. Smallest mRNA length that can be translated - (reply: 3)
166. wrong insert? right insert?? - (reply: 2)
167. Help~ Incorrect constructs in cloning - (reply: 3)
168. Problems with ligation and transformation - (reply: 2)
169. Transformation of Restricted plasmid - (reply: 3)
170. Scrambled sequence? - (reply: 3)
171. Why is my insert missing from my plasmid? - (reply: 4)
172. PCR Profile for ligation - (reply: 3)
173. Blunt end is ligating to sticky end? - (reply: 2)
174. SNP in the promoter region - (reply: 1)
175. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
176. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
177. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
178. New ordered Restriction Enzymes not working, need help! - (reply: 2)
179. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
180. Transfection but no expression - (reply: 4)