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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Failed TOPO cloning - what happened? - (reply: 9)
152. TOPO cloning after Phusion-PCR - (reply: 1)
153. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
154. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
155. Deleting 7Mbp chromosomal region - (reply: 3)
156. Electrophoresis samples and volumes - (reply: 1)
157. Gene cloning with unknown sequence - (reply: 6)
158. Checking for efficieny competent cells - (reply: 1)
159. Troubles with Fusion PCR - (reply: 1)
160. Does this look alright? - (reply: 3)
161. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
162. Cloning pDsRed-Monomer-N1 - (reply: 1)
163. forgot to heat shock - (reply: 6)
164. Ligation left for weekend at room temp - (reply: 4)
165. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
166. Problem in plasmid digestion after transformation - (reply: 4)
167. Lac Promoter activity in P. aeruginosa - (reply: 1)
168. Resctriction analysis - (reply: 5)
169. Please comment on my Cloning Plan - (reply: 1)
170. Gibson/SLIC question - (reply: 1)
171. How to determine where, in the genome, my transgene inserted - (reply: 4)
172. no colonies, ever! - (reply: 5)
173. fluorescent tag on membrane protein - (reply: 2)
174. How can addgene sell plasmids with patented technology - (reply: 2)
175. no insert issue - (reply: 9)
176. Considerations on the design of a transgene - (reply: 1)
177. How to obtain Vectors used in articles - (reply: 5)
178. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
179. How efficient is the 2A sequence - (reply: 2)
180. Transformation keeps failing - (reply: 2)