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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Genomic -Lactamase? Blue White screening not working - (reply: 1)
152. direction of the expression units in multi-gene vectors - (reply: 1)
153. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
154. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
155. Measuring pcr fragments in a gel - (reply: 1)
156. gel electrophoresis - (reply: 4)
157. three questions about CRISPR knowout - (reply: 2)
158. Help needed for Ligation/Transformation - (reply: 1)
159. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
160. ClaI restriction enzyme efficency - (reply: 1)
161. Blunt end cloning - (reply: 12)
162. sequantial digest - (reply: 25)
163. Temperature sensitivity of Plasmids - (reply: 1)
164. DMSO in restriction digest - (reply: 1)
165. Molecular cloning - (reply: 5)
166. Nuclease free water for cloning - (reply: 8)
167. Directional cloning 2bps overhang - (reply: 3)
168. I need help designing homology arms for CRISPR - (reply: 3)
169. Partial digest XhoI NotI NEB - (reply: 6)
170. removing part from plasmid - (reply: 18)
171. Start codon after promoter - (reply: 1)
172. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
173. Purpose of PolyA in cloning - (reply: 1)
174. Ligation failure? I ran some on a gel - (reply: 6)
175. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
176. Cloning into pVIVO2 vector - (reply: 8)
177. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
178. Cloning worked, but the expression vector is weird - (reply: 6)
179. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
180. site directed mutagenesis - (reply: 3)