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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Ligation-transformation...Never faced such problem ever - (reply: 6)
152. Can multiple genes follow only one promoter? - (reply: 1)
153. Restriction Digest Question - (reply: 4)
154. Problem with Large vector & very small insert - (reply: 1)
155. Storage life of plasmid DNA in water - (reply: 5)
156. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
157. Site-directed mutagenesis failed - (reply: 20)
158. Insert & vector too dilute to perform ligation - (reply: 3)
159. Cloning Problems - (reply: 2)
160. Suitable templates for in vitro transcription - (reply: 1)
161. Different ways to ensure the successful cloning? - (reply: 2)
162. Plasmid DNA digestion problem - (reply: 3)
163. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
164. Single codon insert - (reply: 3)
165. cloning- ligation - (reply: 3)
166. Sticky and blunt ligation issue - (reply: 6)
167. Handling DEPC under nitrogen/argon - (reply: 3)
168. Incubation period after heat shock at transformation--reason? - (reply: 3)
169. PCR product sequencing - (reply: 3)
170. What is the optimal time for transfection efficiency measuring? - (reply: 1)
171. i couldn't get the band of target gene after ligation - (reply: 9)
172. Minimum homology bases needed for homologous recombination - (reply: 1)
173. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
174. Expansion of commercial competent bacterial cell lines? - (reply: 2)
175. Manipulation of target DNA fragments - (reply: 1)
176. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
177. Appearance of a salty plasmid preparation - (reply: 1)
178. origami bacteria transformation - (reply: 5)
179. Smallest mRNA length that can be translated - (reply: 3)
180. wrong insert? right insert?? - (reply: 2)