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Top : New Forum Archives (2009-): : Molecular-Cloning
151. cDNA Library Construction by using kits and Sequencing - (reply: 1)
152. Looking for software that permutes codons to find new restriction sites - (reply: 4)
153. Transforming dna from agarose gel - (reply: 3)
154. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
155. ccdb toxin - (reply: 15)
156. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
157. annealing oligos - (reply: 3)
158. Does a restriction enzyme cut at a related site? - (reply: 5)
159. primers for cloning - (reply: 1)
160. Vector-insert ratio - (reply: 6)
161. Cloning contamination - (reply: 3)
162. plasmid construction - (reply: 2)
163. primer design with restriction enzyme - (reply: 1)
164. vector propagation in E coli - (reply: 1)
165. Restriction to remove background - (reply: 4)
166. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
167. Restriction and ligation time interval - (reply: 1)
168. Problem shrna cloning, bad sequencing results - (reply: 1)
169. Lentivirus copy number - (reply: 7)
170. Genomic -Lactamase? Blue White screening not working - (reply: 1)
171. direction of the expression units in multi-gene vectors - (reply: 1)
172. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
173. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
174. Measuring pcr fragments in a gel - (reply: 1)
175. gel electrophoresis - (reply: 4)
176. three questions about CRISPR knowout - (reply: 2)
177. Help needed for Ligation/Transformation - (reply: 1)
178. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
179. ClaI restriction enzyme efficency - (reply: 1)
180. Blunt end cloning - (reply: 12)