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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Cloning two genes in the same plasmid with one promotor - (reply: 4)
152. Sending a TOPO-construct for sequencing? - (reply: 1)
153. Is this counter-productive cloning or not? - (reply: 8)
154. Colony screening - (reply: 1)
155. Failed cloning for 1month - TOPO - (reply: 7)
156. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
157. Which method for DNA purification b4 cloning - (reply: 4)
158. Purification for TOPO - (reply: 1)
159. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
160. Failed TOPO cloning - what happened? - (reply: 9)
161. TOPO cloning after Phusion-PCR - (reply: 1)
162. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
163. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
164. Deleting 7Mbp chromosomal region - (reply: 3)
165. Electrophoresis samples and volumes - (reply: 1)
166. Gene cloning with unknown sequence - (reply: 6)
167. Checking for efficieny competent cells - (reply: 1)
168. Troubles with Fusion PCR - (reply: 1)
169. Does this look alright? - (reply: 3)
170. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
171. Cloning pDsRed-Monomer-N1 - (reply: 1)
172. forgot to heat shock - (reply: 6)
173. Ligation left for weekend at room temp - (reply: 4)
174. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
175. Problem in plasmid digestion after transformation - (reply: 4)
176. Lac Promoter activity in P. aeruginosa - (reply: 1)
177. Resctriction analysis - (reply: 5)
178. Please comment on my Cloning Plan - (reply: 1)
179. Gibson/SLIC question - (reply: 1)
180. How to determine where, in the genome, my transgene inserted - (reply: 4)