Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
151. vector propagation in E coli - (reply: 1)
152. Restriction to remove background - (reply: 4)
153. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
154. Restriction and ligation time interval - (reply: 1)
155. Problem shrna cloning, bad sequencing results - (reply: 1)
156. Lentivirus copy number - (reply: 7)
157. Genomic -Lactamase? Blue White screening not working - (reply: 1)
158. direction of the expression units in multi-gene vectors - (reply: 1)
159. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
160. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
161. Measuring pcr fragments in a gel - (reply: 1)
162. gel electrophoresis - (reply: 4)
163. three questions about CRISPR knowout - (reply: 2)
164. Help needed for Ligation/Transformation - (reply: 1)
165. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
166. ClaI restriction enzyme efficency - (reply: 1)
167. Blunt end cloning - (reply: 12)
168. sequantial digest - (reply: 25)
169. Temperature sensitivity of Plasmids - (reply: 1)
170. DMSO in restriction digest - (reply: 1)
171. Molecular cloning - (reply: 5)
172. Nuclease free water for cloning - (reply: 8)
173. Directional cloning 2bps overhang - (reply: 3)
174. I need help designing homology arms for CRISPR - (reply: 3)
175. Partial digest XhoI NotI NEB - (reply: 6)
176. removing part from plasmid - (reply: 18)
177. Start codon after promoter - (reply: 1)
178. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
179. Purpose of PolyA in cloning - (reply: 1)
180. Ligation failure? I ran some on a gel - (reply: 6)