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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
152. annealing oligos - (reply: 3)
153. Does a restriction enzyme cut at a related site? - (reply: 5)
154. primers for cloning - (reply: 1)
155. Vector-insert ratio - (reply: 6)
156. Cloning contamination - (reply: 3)
157. plasmid construction - (reply: 2)
158. primer design with restriction enzyme - (reply: 1)
159. vector propagation in E coli - (reply: 1)
160. Restriction to remove background - (reply: 4)
161. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
162. Restriction and ligation time interval - (reply: 1)
163. Problem shrna cloning, bad sequencing results - (reply: 1)
164. Lentivirus copy number - (reply: 7)
165. Genomic -Lactamase? Blue White screening not working - (reply: 1)
166. direction of the expression units in multi-gene vectors - (reply: 1)
167. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
168. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
169. Measuring pcr fragments in a gel - (reply: 1)
170. gel electrophoresis - (reply: 4)
171. three questions about CRISPR knowout - (reply: 2)
172. Help needed for Ligation/Transformation - (reply: 1)
173. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
174. ClaI restriction enzyme efficency - (reply: 1)
175. Blunt end cloning - (reply: 12)
176. sequantial digest - (reply: 25)
177. Temperature sensitivity of Plasmids - (reply: 1)
178. DMSO in restriction digest - (reply: 1)
179. Molecular cloning - (reply: 5)
180. Nuclease free water for cloning - (reply: 8)