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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
152. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
153. Measuring pcr fragments in a gel - (reply: 1)
154. gel electrophoresis - (reply: 4)
155. three questions about CRISPR knowout - (reply: 2)
156. Help needed for Ligation/Transformation - (reply: 1)
157. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
158. ClaI restriction enzyme efficency - (reply: 1)
159. Blunt end cloning - (reply: 12)
160. sequantial digest - (reply: 25)
161. Temperature sensitivity of Plasmids - (reply: 1)
162. DMSO in restriction digest - (reply: 1)
163. Molecular cloning - (reply: 5)
164. Nuclease free water for cloning - (reply: 8)
165. Directional cloning 2bps overhang - (reply: 3)
166. I need help designing homology arms for CRISPR - (reply: 3)
167. Partial digest XhoI NotI NEB - (reply: 6)
168. removing part from plasmid - (reply: 18)
169. Start codon after promoter - (reply: 1)
170. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
171. Purpose of PolyA in cloning - (reply: 1)
172. Ligation failure? I ran some on a gel - (reply: 6)
173. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
174. Cloning into pVIVO2 vector - (reply: 8)
175. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
176. Cloning worked, but the expression vector is weird - (reply: 6)
177. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
178. site directed mutagenesis - (reply: 3)
179. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
180. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)