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151. Help designing primers for use in In-fushion cloning - (reply: 4)
152. Repeat of desired mutation in cloning product - (reply: 1)
153. vector dephosphorylation - (reply: 5)
154. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
155. DH10BAC - (reply: 3)
156. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
157. protein is not expessing in HEK 293 cells - (reply: 2)
158. Restriction problems - (reply: 4)
159. cloning a gene in to a plasmid - (reply: 5)
160. no product on gel after restriction digestion - (reply: 3)
161. Gateway technology. - (reply: 4)
162. Primer concentration for cDNA synthesis using GSP - (reply: 1)
163. cloning from cDNA but got a much shorter PCR product - (reply: 4)
164. GeneArt vs Quikchange - (reply: 1)
165. why do some colonies keep growing in the chiller? - (reply: 7)
166. Cloning & Expression Problem in HEK-293Ts - (reply: 5)
167. Difference between types of bacterial culture broths? - (reply: 4)
168. Co-transfection with 2 plasmids - (reply: 10)
169. Low passage HEK293?? - (reply: 3)
170. Vector Digestion Problem - (reply: 4)
171. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
172. Transformation with a big plasmid - (reply: 4)
173. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
174. Three way ligation problem - (reply: 6)
175. problems with recovery of dna from gel and ligation - (reply: 5)
176. Colony PCR and digestion with KpnI - (reply: 10)
177. Greenish tinge to bacterial culture - (reply: 3)
178. SURE E. coli competent cells - (reply: 1)
179. Ligation - (reply: 20)
180. E. coli growing on plates and bot not in LB medium - (reply: 10)
152. Repeat of desired mutation in cloning product - (reply: 1)
153. vector dephosphorylation - (reply: 5)
154. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
155. DH10BAC - (reply: 3)
156. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
157. protein is not expessing in HEK 293 cells - (reply: 2)
158. Restriction problems - (reply: 4)
159. cloning a gene in to a plasmid - (reply: 5)
160. no product on gel after restriction digestion - (reply: 3)
161. Gateway technology. - (reply: 4)
162. Primer concentration for cDNA synthesis using GSP - (reply: 1)
163. cloning from cDNA but got a much shorter PCR product - (reply: 4)
164. GeneArt vs Quikchange - (reply: 1)
165. why do some colonies keep growing in the chiller? - (reply: 7)
166. Cloning & Expression Problem in HEK-293Ts - (reply: 5)
167. Difference between types of bacterial culture broths? - (reply: 4)
168. Co-transfection with 2 plasmids - (reply: 10)
169. Low passage HEK293?? - (reply: 3)
170. Vector Digestion Problem - (reply: 4)
171. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
172. Transformation with a big plasmid - (reply: 4)
173. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
174. Three way ligation problem - (reply: 6)
175. problems with recovery of dna from gel and ligation - (reply: 5)
176. Colony PCR and digestion with KpnI - (reply: 10)
177. Greenish tinge to bacterial culture - (reply: 3)
178. SURE E. coli competent cells - (reply: 1)
179. Ligation - (reply: 20)
180. E. coli growing on plates and bot not in LB medium - (reply: 10)