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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Working backwards from a construct? - (reply: 1)
152. disappearing bases - (reply: 4)
153. Mutagenesis PCR - (reply: 9)
154. Digest two sites located next to each other - (reply: 5)
155. cDNA Library Construction by using kits and Sequencing - (reply: 1)
156. Looking for software that permutes codons to find new restriction sites - (reply: 4)
157. Transforming dna from agarose gel - (reply: 3)
158. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
159. ccdb toxin - (reply: 15)
160. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
161. annealing oligos - (reply: 3)
162. Does a restriction enzyme cut at a related site? - (reply: 5)
163. primers for cloning - (reply: 1)
164. Vector-insert ratio - (reply: 6)
165. Cloning contamination - (reply: 3)
166. plasmid construction - (reply: 2)
167. primer design with restriction enzyme - (reply: 1)
168. vector propagation in E coli - (reply: 1)
169. Restriction to remove background - (reply: 4)
170. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
171. Restriction and ligation time interval - (reply: 1)
172. Problem shrna cloning, bad sequencing results - (reply: 1)
173. Lentivirus copy number - (reply: 7)
174. Genomic -Lactamase? Blue White screening not working - (reply: 1)
175. direction of the expression units in multi-gene vectors - (reply: 1)
176. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
177. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
178. Measuring pcr fragments in a gel - (reply: 1)
179. gel electrophoresis - (reply: 4)
180. three questions about CRISPR knowout - (reply: 2)