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Top : New Forum Archives (2009-): : Molecular-Cloning
151. primer design with restriction enzyme - (reply: 1)
152. vector propagation in E coli - (reply: 1)
153. Restriction to remove background - (reply: 4)
154. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
155. Restriction and ligation time interval - (reply: 1)
156. Problem shrna cloning, bad sequencing results - (reply: 1)
157. Lentivirus copy number - (reply: 7)
158. Genomic -Lactamase? Blue White screening not working - (reply: 1)
159. direction of the expression units in multi-gene vectors - (reply: 1)
160. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
161. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
162. Measuring pcr fragments in a gel - (reply: 1)
163. gel electrophoresis - (reply: 4)
164. three questions about CRISPR knowout - (reply: 2)
165. Help needed for Ligation/Transformation - (reply: 1)
166. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
167. ClaI restriction enzyme efficency - (reply: 1)
168. Blunt end cloning - (reply: 12)
169. sequantial digest - (reply: 25)
170. Temperature sensitivity of Plasmids - (reply: 1)
171. DMSO in restriction digest - (reply: 1)
172. Molecular cloning - (reply: 5)
173. Nuclease free water for cloning - (reply: 8)
174. Directional cloning 2bps overhang - (reply: 3)
175. I need help designing homology arms for CRISPR - (reply: 3)
176. Partial digest XhoI NotI NEB - (reply: 6)
177. removing part from plasmid - (reply: 18)
178. Start codon after promoter - (reply: 1)
179. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
180. Purpose of PolyA in cloning - (reply: 1)