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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Restriction to remove background - (reply: 4)
152. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
153. Restriction and ligation time interval - (reply: 1)
154. Problem shrna cloning, bad sequencing results - (reply: 1)
155. Lentivirus copy number - (reply: 7)
156. Genomic -Lactamase? Blue White screening not working - (reply: 1)
157. direction of the expression units in multi-gene vectors - (reply: 1)
158. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
159. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
160. Measuring pcr fragments in a gel - (reply: 1)
161. gel electrophoresis - (reply: 4)
162. three questions about CRISPR knowout - (reply: 2)
163. Help needed for Ligation/Transformation - (reply: 1)
164. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
165. ClaI restriction enzyme efficency - (reply: 1)
166. Blunt end cloning - (reply: 12)
167. sequantial digest - (reply: 25)
168. Temperature sensitivity of Plasmids - (reply: 1)
169. DMSO in restriction digest - (reply: 1)
170. Molecular cloning - (reply: 5)
171. Nuclease free water for cloning - (reply: 8)
172. Directional cloning 2bps overhang - (reply: 3)
173. I need help designing homology arms for CRISPR - (reply: 3)
174. Partial digest XhoI NotI NEB - (reply: 6)
175. removing part from plasmid - (reply: 18)
176. Start codon after promoter - (reply: 1)
177. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
178. Purpose of PolyA in cloning - (reply: 1)
179. Ligation failure? I ran some on a gel - (reply: 6)
180. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)