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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Measuring pcr fragments in a gel - (reply: 1)
152. gel electrophoresis - (reply: 4)
153. three questions about CRISPR knowout - (reply: 2)
154. Help needed for Ligation/Transformation - (reply: 1)
155. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
156. ClaI restriction enzyme efficency - (reply: 1)
157. Blunt end cloning - (reply: 12)
158. sequantial digest - (reply: 25)
159. Temperature sensitivity of Plasmids - (reply: 1)
160. DMSO in restriction digest - (reply: 1)
161. Molecular cloning - (reply: 5)
162. Nuclease free water for cloning - (reply: 8)
163. Directional cloning 2bps overhang - (reply: 3)
164. I need help designing homology arms for CRISPR - (reply: 3)
165. Partial digest XhoI NotI NEB - (reply: 6)
166. removing part from plasmid - (reply: 18)
167. Start codon after promoter - (reply: 1)
168. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
169. Purpose of PolyA in cloning - (reply: 1)
170. Ligation failure? I ran some on a gel - (reply: 6)
171. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
172. Cloning into pVIVO2 vector - (reply: 8)
173. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
174. Cloning worked, but the expression vector is weird - (reply: 6)
175. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
176. site directed mutagenesis - (reply: 3)
177. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
178. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
179. restriction enzymes/sites to avoid - (reply: 4)
180. Using pBABE plasmids for regular transfection - (reply: 1)