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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Gateway vectors - (reply: 3)
152. question on gateway - (reply: 1)
153. Cloning problem still not solved - (reply: 2)
154. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
155. Insert 30nt at C-terminal - (reply: 1)
156. How to store a ligation reaction over the weekend? - (reply: 3)
157. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
158. How homologous do homology arms need to be? - (reply: 3)
159. cloning pEGFP-N1 - (reply: 1)
160. Loss of DNA after restriction digestion cleanup - (reply: 2)
161. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
162. separating mixture of plamids - (reply: 7)
163. Kozak sequence and mammalian expression vector - (reply: 3)
164. GFP vector - (reply: 1)
165. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
166. no colony - (reply: 3)
167. Cloning beginner and clueless! - (reply: 1)
168. Which epitope tag to use for ChIP? - (reply: 1)
169. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
170. Ligation-transformation...Never faced such problem ever - (reply: 6)
171. Can multiple genes follow only one promoter? - (reply: 1)
172. Restriction Digest Question - (reply: 4)
173. Problem with Large vector & very small insert - (reply: 1)
174. Storage life of plasmid DNA in water - (reply: 5)
175. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
176. Site-directed mutagenesis failed - (reply: 20)
177. Insert & vector too dilute to perform ligation - (reply: 3)
178. Cloning Problems - (reply: 2)
179. Suitable templates for in vitro transcription - (reply: 1)
180. Different ways to ensure the successful cloning? - (reply: 2)