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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Digest two sites located next to each other - (reply: 5)
152. cDNA Library Construction by using kits and Sequencing - (reply: 1)
153. Looking for software that permutes codons to find new restriction sites - (reply: 4)
154. Transforming dna from agarose gel - (reply: 3)
155. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
156. ccdb toxin - (reply: 15)
157. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
158. annealing oligos - (reply: 3)
159. Does a restriction enzyme cut at a related site? - (reply: 5)
160. primers for cloning - (reply: 1)
161. Vector-insert ratio - (reply: 6)
162. Cloning contamination - (reply: 3)
163. plasmid construction - (reply: 2)
164. primer design with restriction enzyme - (reply: 1)
165. vector propagation in E coli - (reply: 1)
166. Restriction to remove background - (reply: 4)
167. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
168. Restriction and ligation time interval - (reply: 1)
169. Problem shrna cloning, bad sequencing results - (reply: 1)
170. Lentivirus copy number - (reply: 7)
171. Genomic -Lactamase? Blue White screening not working - (reply: 1)
172. direction of the expression units in multi-gene vectors - (reply: 1)
173. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
174. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
175. Measuring pcr fragments in a gel - (reply: 1)
176. gel electrophoresis - (reply: 4)
177. three questions about CRISPR knowout - (reply: 2)
178. Help needed for Ligation/Transformation - (reply: 1)
179. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
180. ClaI restriction enzyme efficency - (reply: 1)