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Top : New Forum Archives (2009-): : Molecular-Cloning
151. Temperature sensitivity of Plasmids - (reply: 1)
152. DMSO in restriction digest - (reply: 1)
153. Molecular cloning - (reply: 5)
154. Nuclease free water for cloning - (reply: 8)
155. Directional cloning 2bps overhang - (reply: 3)
156. I need help designing homology arms for CRISPR - (reply: 3)
157. Partial digest XhoI NotI NEB - (reply: 6)
158. removing part from plasmid - (reply: 18)
159. Start codon after promoter - (reply: 1)
160. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
161. Purpose of PolyA in cloning - (reply: 1)
162. Ligation failure? I ran some on a gel - (reply: 6)
163. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
164. Cloning into pVIVO2 vector - (reply: 8)
165. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
166. Cloning worked, but the expression vector is weird - (reply: 6)
167. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
168. site directed mutagenesis - (reply: 3)
169. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
170. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
171. restriction enzymes/sites to avoid - (reply: 4)
172. Using pBABE plasmids for regular transfection - (reply: 1)
173. Ubiquitin promoter cloning - (reply: 1)
174. Cloning two genes in the same plasmid with one promotor - (reply: 4)
175. Sending a TOPO-construct for sequencing? - (reply: 1)
176. Is this counter-productive cloning or not? - (reply: 8)
177. Colony screening - (reply: 1)
178. Failed cloning for 1month - TOPO - (reply: 7)
179. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
180. Which method for DNA purification b4 cloning - (reply: 4)