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Top : New Forum Archives (2009-): : Molecular-Cloning
1531. Help cloning!!! - As usual cloning is not working! (reply: 11)
1532. pSuper cloning problems - (reply: 1)
1533. problem with plasmid extraction - (reply: 3)
1534. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1535. Smear in my restriction digest - (reply: 12)
1536. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1537. How to transfect two plasmids in a cell - (reply: 10)
1538. Double Digest using enzymes from diff companies - (reply: 4)
1539. Ligation problem (no colonies, but on negative control) - (reply: 3)
1540. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1541. SDM - (reply: 3)
1542. inframe problem - (reply: 1)
1543. Touchdown PCR - (reply: 1)
1544. Adding A tails? - (reply: 3)
1545. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1546. Determine annealing temperature of primers - (reply: 14)
1547. Bacteria pellet has almost-black region - (reply: 10)
1548. no ligation !!!!! - (reply: 6)
1549. How to clean up restriction enzyme digestion - (reply: 8)
1550. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1551. GPCR - (reply: 1)
1552. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1553. finding the suitable RE - first timer (reply: 6)
1554. TOPO TA Problems - TOPO TA Problems (reply: 8)
1555. about antibiotic addition - (reply: 4)
1556. about chloramphenicol and L.lactis - (reply: 1)
1557. ligation - (reply: 5)
1558. Losing insert from pGEM? - (reply: 2)
1559. How to concentrate lenti or retroviral particules - (reply: 3)
1560. wierd clot in LB culture - (reply: 5)