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Top : New Forum Archives (2009-): : Molecular-Cloning
1531. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
1532. Cloning Conundrum - (reply: 7)
1533. Sewing PCR - (reply: 3)
1534. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
1535. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
1536. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
1537. Colony PCR - Next step problems (reply: 6)
1538. optimum temperature for ligation - (reply: 14)
1539. Please Help with Lentiviral Cloning - (reply: 1)
1540. plasmid-rest. dign. - (reply: 2)
1541. Help with cloning!. - Cloning problems (reply: 20)
1542. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
1543. Cloning PCR fragment - (reply: 1)
1544. molecular cloning - (reply: 2)
1545. DNA cloning - (reply: 4)
1546. Help with transformation - (reply: 3)
1547. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1548. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1549. Help cloning!!! - As usual cloning is not working! (reply: 11)
1550. pSuper cloning problems - (reply: 1)
1551. problem with plasmid extraction - (reply: 3)
1552. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1553. Smear in my restriction digest - (reply: 12)
1554. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1555. How to transfect two plasmids in a cell - (reply: 9)
1556. Double Digest using enzymes from diff companies - (reply: 4)
1557. Ligation problem (no colonies, but on negative control) - (reply: 3)
1558. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1559. SDM - (reply: 3)
1560. inframe problem - (reply: 1)