Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1531. Colony PCR - Next step problems (reply: 6)
1532. optimum temperature for ligation - (reply: 14)
1533. Please Help with Lentiviral Cloning - (reply: 1)
1534. plasmid-rest. dign. - (reply: 2)
1535. Help with cloning!. - Cloning problems (reply: 20)
1536. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
1537. Cloning PCR fragment - (reply: 1)
1538. molecular cloning - (reply: 2)
1539. DNA cloning - (reply: 4)
1540. Help with transformation - (reply: 3)
1541. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1542. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1543. Help cloning!!! - As usual cloning is not working! (reply: 11)
1544. pSuper cloning problems - (reply: 1)
1545. problem with plasmid extraction - (reply: 3)
1546. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1547. Smear in my restriction digest - (reply: 12)
1548. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1549. How to transfect two plasmids in a cell - (reply: 9)
1550. Double Digest using enzymes from diff companies - (reply: 4)
1551. Ligation problem (no colonies, but on negative control) - (reply: 3)
1552. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1553. SDM - (reply: 3)
1554. inframe problem - (reply: 1)
1555. Touchdown PCR - (reply: 1)
1556. Adding A tails? - (reply: 3)
1557. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1558. Determine annealing temperature of primers - (reply: 14)
1559. Bacteria pellet has almost-black region - (reply: 10)
1560. no ligation !!!!! - (reply: 6)