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Top : New Forum Archives (2009-): : Molecular-Cloning
1531. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
1532. Colony PCR - Next step problems (reply: 6)
1533. optimum temperature for ligation - (reply: 14)
1534. Please Help with Lentiviral Cloning - (reply: 1)
1535. plasmid-rest. dign. - (reply: 2)
1536. Help with cloning!. - Cloning problems (reply: 20)
1537. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
1538. Cloning PCR fragment - (reply: 1)
1539. molecular cloning - (reply: 2)
1540. DNA cloning - (reply: 4)
1541. Help with transformation - (reply: 3)
1542. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1543. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1544. Help cloning!!! - As usual cloning is not working! (reply: 11)
1545. pSuper cloning problems - (reply: 1)
1546. problem with plasmid extraction - (reply: 3)
1547. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1548. Smear in my restriction digest - (reply: 12)
1549. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1550. How to transfect two plasmids in a cell - (reply: 9)
1551. Double Digest using enzymes from diff companies - (reply: 4)
1552. Ligation problem (no colonies, but on negative control) - (reply: 3)
1553. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1554. SDM - (reply: 3)
1555. inframe problem - (reply: 1)
1556. Touchdown PCR - (reply: 1)
1557. Adding A tails? - (reply: 3)
1558. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1559. Determine annealing temperature of primers - (reply: 14)
1560. Bacteria pellet has almost-black region - (reply: 10)