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Top : New Forum Archives (2009-): : Molecular-Cloning
1531. Please Help with Lentiviral Cloning - (reply: 1)
1532. plasmid-rest. dign. - (reply: 2)
1533. Help with cloning!. - Cloning problems (reply: 20)
1534. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
1535. Cloning PCR fragment - (reply: 1)
1536. molecular cloning - (reply: 2)
1537. DNA cloning - (reply: 4)
1538. Help with transformation - (reply: 3)
1539. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1540. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1541. Help cloning!!! - As usual cloning is not working! (reply: 11)
1542. pSuper cloning problems - (reply: 1)
1543. problem with plasmid extraction - (reply: 3)
1544. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1545. Smear in my restriction digest - (reply: 12)
1546. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1547. How to transfect two plasmids in a cell - (reply: 9)
1548. Double Digest using enzymes from diff companies - (reply: 4)
1549. Ligation problem (no colonies, but on negative control) - (reply: 3)
1550. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1551. SDM - (reply: 3)
1552. inframe problem - (reply: 1)
1553. Touchdown PCR - (reply: 1)
1554. Adding A tails? - (reply: 3)
1555. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1556. Determine annealing temperature of primers - (reply: 14)
1557. Bacteria pellet has almost-black region - (reply: 10)
1558. no ligation !!!!! - (reply: 6)
1559. How to clean up restriction enzyme digestion - (reply: 8)
1560. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)