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Top : New Forum Archives (2009-): : Molecular-Cloning
1531. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1532. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1533. Help cloning!!! - As usual cloning is not working! (reply: 11)
1534. pSuper cloning problems - (reply: 1)
1535. problem with plasmid extraction - (reply: 3)
1536. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1537. Smear in my restriction digest - (reply: 12)
1538. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
1539. How to transfect two plasmids in a cell - (reply: 9)
1540. Double Digest using enzymes from diff companies - (reply: 4)
1541. Ligation problem (no colonies, but on negative control) - (reply: 3)
1542. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1543. SDM - (reply: 3)
1544. inframe problem - (reply: 1)
1545. Touchdown PCR - (reply: 1)
1546. Adding A tails? - (reply: 3)
1547. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1548. Determine annealing temperature of primers - (reply: 14)
1549. Bacteria pellet has almost-black region - (reply: 10)
1550. no ligation !!!!! - (reply: 6)
1551. How to clean up restriction enzyme digestion - (reply: 8)
1552. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1553. GPCR - (reply: 1)
1554. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1555. finding the suitable RE - first timer (reply: 6)
1556. TOPO TA Problems - TOPO TA Problems (reply: 8)
1557. about antibiotic addition - (reply: 4)
1558. about chloramphenicol and L.lactis - (reply: 1)
1559. ligation - (reply: 5)
1560. Losing insert from pGEM? - (reply: 2)