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1531. need help on isolation of intron of gene - (reply: 1)
1532. Problems with my ligation reaction - (reply: 5)
1533. primer design - really necessary? (reply: 2)
1534. Genomic DNA and PCR - (reply: 4)
1535. Cloning done....sequence of the insert for the expression vector - (reply: 1)
1536. His Tag protein - (reply: 4)
1537. cloning problem - (reply: 3)
1538. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
1539. Cloning Conundrum - (reply: 7)
1540. Sewing PCR - (reply: 3)
1541. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
1542. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
1543. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
1544. Colony PCR - Next step problems (reply: 6)
1545. optimum temperature for ligation - (reply: 14)
1546. Please Help with Lentiviral Cloning - (reply: 1)
1547. plasmid-rest. dign. - (reply: 2)
1548. Help with cloning!. - Cloning problems (reply: 20)
1549. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
1550. Cloning PCR fragment - (reply: 1)
1551. molecular cloning - (reply: 2)
1552. DNA cloning - (reply: 4)
1553. Help with transformation - (reply: 3)
1554. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1555. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1556. Help cloning!!! - As usual cloning is not working! (reply: 11)
1557. pSuper cloning problems - (reply: 1)
1558. problem with plasmid extraction - (reply: 3)
1559. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1560. Smear in my restriction digest - (reply: 12)
1532. Problems with my ligation reaction - (reply: 5)
1533. primer design - really necessary? (reply: 2)
1534. Genomic DNA and PCR - (reply: 4)
1535. Cloning done....sequence of the insert for the expression vector - (reply: 1)
1536. His Tag protein - (reply: 4)
1537. cloning problem - (reply: 3)
1538. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
1539. Cloning Conundrum - (reply: 7)
1540. Sewing PCR - (reply: 3)
1541. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
1542. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
1543. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
1544. Colony PCR - Next step problems (reply: 6)
1545. optimum temperature for ligation - (reply: 14)
1546. Please Help with Lentiviral Cloning - (reply: 1)
1547. plasmid-rest. dign. - (reply: 2)
1548. Help with cloning!. - Cloning problems (reply: 20)
1549. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
1550. Cloning PCR fragment - (reply: 1)
1551. molecular cloning - (reply: 2)
1552. DNA cloning - (reply: 4)
1553. Help with transformation - (reply: 3)
1554. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
1555. Changing Restriction Enzyme Sites with oligos - (reply: 2)
1556. Help cloning!!! - As usual cloning is not working! (reply: 11)
1557. pSuper cloning problems - (reply: 1)
1558. problem with plasmid extraction - (reply: 3)
1559. random cleavage of plasmids - insertional mutagenesis (reply: 2)
1560. Smear in my restriction digest - (reply: 12)