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Top : New Forum Archives (2009-): : Molecular-Cloning
1471. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1472. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1473. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1474. choosing restriction sites - (reply: 5)
1475. Tag insertion - (reply: 5)
1476. Transduction - (reply: 3)
1477. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1478. my colonies don't have the insert, how is this possible? - (reply: 7)
1479. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1480. PCR problem from transformed TOPO TA vector - (reply: 3)
1481. nondenaturing PAGE to separate dna - (reply: 4)
1482. why sometimes big or small colonies? - (reply: 16)
1483. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1484. wrong bands after T-vector cloning - (reply: 3)
1485. urgent help regarding wanner method trouble shooting - (reply: 2)
1486. two step digestion - (reply: 6)
1487. Storage of invitrogen top10 cells - (reply: 11)
1488. Self-Ligation of digested vector - (reply: 2)
1489. Help with cloning - (reply: 6)
1490. Ligation issue: Does vector size matter? - (reply: 10)
1491. Help for T-A cloning - (reply: 15)
1492. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1493. Some colonies, but not enough... - (reply: 7)
1494. transformation problem - (reply: 3)
1495. Plasmid sequence - (reply: 1)
1496. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1497. help wih the ligation - (reply: 7)
1498. PCR screening of transformed bacterial colony - (reply: 6)
1499. No colony for oligo cloning - (reply: 9)
1500. cloning - affect on c-terminal (reply: 1)