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Top : New Forum Archives (2009-): : Molecular-Cloning
1471. Self-Ligation of digested vector - (reply: 2)
1472. Help with cloning - (reply: 6)
1473. Ligation issue: Does vector size matter? - (reply: 10)
1474. Help for T-A cloning - (reply: 15)
1475. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1476. Some colonies, but not enough... - (reply: 7)
1477. transformation problem - (reply: 3)
1478. Plasmid sequence - (reply: 1)
1479. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1480. help wih the ligation - (reply: 7)
1481. PCR screening of transformed bacterial colony - (reply: 6)
1482. No colony for oligo cloning - (reply: 9)
1483. cloning - affect on c-terminal (reply: 1)
1484. Growing of TOP10 chemically competent cells - (reply: 4)
1485. Stable Cell Selection with Neomycin - (reply: 4)
1486. lpzzzzz that is so important to my PhD. - (reply: 1)
1487. need guidence for cdna library - screen library (reply: 5)
1488. No product after restriction digestion - (reply: 26)
1489. protein overexpression question. - (reply: 4)
1490. Basic doubt on restriction digestion - (reply: 2)
1491. Transform staph? - (reply: 2)
1492. help for cDNA synthesis - plus RE problem. (reply: 3)
1493. adjacent restriction sites - (reply: 6)
1494. blunt ligation - (reply: 3)
1495. pENTR TOPO cloning - (reply: 1)
1496. where can i ontain the binary vector pCB301? - (reply: 1)
1497. yet one more cloning problem - (reply: 7)
1498. plasmid isolation - (reply: 4)
1499. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
1500. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)