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Top : New Forum Archives (2009-): : Molecular-Cloning
1471. Ligation issue: Does vector size matter? - (reply: 10)
1472. Help for T-A cloning - (reply: 15)
1473. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1474. Some colonies, but not enough... - (reply: 7)
1475. transformation problem - (reply: 3)
1476. Plasmid sequence - (reply: 1)
1477. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1478. help wih the ligation - (reply: 7)
1479. PCR screening of transformed bacterial colony - (reply: 6)
1480. No colony for oligo cloning - (reply: 9)
1481. cloning - affect on c-terminal (reply: 1)
1482. Growing of TOP10 chemically competent cells - (reply: 4)
1483. Stable Cell Selection with Neomycin - (reply: 4)
1484. lpzzzzz that is so important to my PhD. - (reply: 1)
1485. need guidence for cdna library - screen library (reply: 5)
1486. No product after restriction digestion - (reply: 26)
1487. protein overexpression question. - (reply: 4)
1488. Basic doubt on restriction digestion - (reply: 2)
1489. Transform staph? - (reply: 2)
1490. help for cDNA synthesis - plus RE problem. (reply: 3)
1491. adjacent restriction sites - (reply: 6)
1492. blunt ligation - (reply: 3)
1493. pENTR TOPO cloning - (reply: 1)
1494. where can i ontain the binary vector pCB301? - (reply: 1)
1495. yet one more cloning problem - (reply: 7)
1496. plasmid isolation - (reply: 4)
1497. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
1498. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
1499. complicated transfection requires professional help - (reply: 3)
1500. strange extra bands on digest of miniprep? - (reply: 5)