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Top : New Forum Archives (2009-): : Molecular-Cloning
1471. my colonies don't have the insert, how is this possible? - (reply: 7)
1472. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1473. PCR problem from transformed TOPO TA vector - (reply: 3)
1474. nondenaturing PAGE to separate dna - (reply: 4)
1475. why sometimes big or small colonies? - (reply: 16)
1476. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1477. wrong bands after T-vector cloning - (reply: 3)
1478. urgent help regarding wanner method trouble shooting - (reply: 2)
1479. two step digestion - (reply: 6)
1480. Storage of invitrogen top10 cells - (reply: 11)
1481. Self-Ligation of digested vector - (reply: 2)
1482. Help with cloning - (reply: 6)
1483. Ligation issue: Does vector size matter? - (reply: 10)
1484. Help for T-A cloning - (reply: 15)
1485. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1486. Some colonies, but not enough... - (reply: 7)
1487. transformation problem - (reply: 3)
1488. Plasmid sequence - (reply: 1)
1489. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1490. help wih the ligation - (reply: 7)
1491. PCR screening of transformed bacterial colony - (reply: 6)
1492. No colony for oligo cloning - (reply: 9)
1493. cloning - affect on c-terminal (reply: 1)
1494. Growing of TOP10 chemically competent cells - (reply: 4)
1495. Stable Cell Selection with Neomycin - (reply: 4)
1496. lpzzzzz that is so important to my PhD. - (reply: 1)
1497. need guidence for cdna library - screen library (reply: 5)
1498. No product after restriction digestion - (reply: 26)
1499. protein overexpression question. - (reply: 4)
1500. Basic doubt on restriction digestion - (reply: 2)