Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1471. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1472. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1473. choosing restriction sites - (reply: 5)
1474. Tag insertion - (reply: 5)
1475. Transduction - (reply: 3)
1476. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1477. my colonies don't have the insert, how is this possible? - (reply: 7)
1478. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1479. PCR problem from transformed TOPO TA vector - (reply: 3)
1480. nondenaturing PAGE to separate dna - (reply: 4)
1481. why sometimes big or small colonies? - (reply: 16)
1482. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1483. wrong bands after T-vector cloning - (reply: 3)
1484. urgent help regarding wanner method trouble shooting - (reply: 2)
1485. two step digestion - (reply: 6)
1486. Storage of invitrogen top10 cells - (reply: 11)
1487. Self-Ligation of digested vector - (reply: 2)
1488. Help with cloning - (reply: 6)
1489. Ligation issue: Does vector size matter? - (reply: 10)
1490. Help for T-A cloning - (reply: 15)
1491. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1492. Some colonies, but not enough... - (reply: 7)
1493. transformation problem - (reply: 3)
1494. Plasmid sequence - (reply: 1)
1495. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1496. help wih the ligation - (reply: 7)
1497. PCR screening of transformed bacterial colony - (reply: 6)
1498. No colony for oligo cloning - (reply: 9)
1499. cloning - affect on c-terminal (reply: 1)
1500. Growing of TOP10 chemically competent cells - (reply: 4)