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Top : New Forum Archives (2009-): : Molecular-Cloning
1471. PCR problem from transformed TOPO TA vector - (reply: 3)
1472. nondenaturing PAGE to separate dna - (reply: 4)
1473. why sometimes big or small colonies? - (reply: 16)
1474. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1475. wrong bands after T-vector cloning - (reply: 3)
1476. urgent help regarding wanner method trouble shooting - (reply: 2)
1477. two step digestion - (reply: 6)
1478. Storage of invitrogen top10 cells - (reply: 11)
1479. Self-Ligation of digested vector - (reply: 2)
1480. Help with cloning - (reply: 6)
1481. Ligation issue: Does vector size matter? - (reply: 10)
1482. Help for T-A cloning - (reply: 15)
1483. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1484. Some colonies, but not enough... - (reply: 7)
1485. transformation problem - (reply: 3)
1486. Plasmid sequence - (reply: 1)
1487. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1488. help wih the ligation - (reply: 7)
1489. PCR screening of transformed bacterial colony - (reply: 6)
1490. No colony for oligo cloning - (reply: 9)
1491. cloning - affect on c-terminal (reply: 1)
1492. Growing of TOP10 chemically competent cells - (reply: 4)
1493. Stable Cell Selection with Neomycin - (reply: 4)
1494. lpzzzzz that is so important to my PhD. - (reply: 1)
1495. need guidence for cdna library - screen library (reply: 5)
1496. No product after restriction digestion - (reply: 26)
1497. protein overexpression question. - (reply: 4)
1498. Basic doubt on restriction digestion - (reply: 2)
1499. Transform staph? - (reply: 2)
1500. help for cDNA synthesis - plus RE problem. (reply: 3)