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1471. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
1472. insert is missing after TA cloning - TA cloning (reply: 6)
1473. glycerol stock - shelf life (reply: 5)
1474. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1475. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1476. cloning problem - molecular cloning (reply: 1)
1477. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1478. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1479. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1480. choosing restriction sites - (reply: 5)
1481. Tag insertion - (reply: 5)
1482. Transduction - (reply: 3)
1483. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1484. my colonies don't have the insert, how is this possible? - (reply: 7)
1485. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1486. PCR problem from transformed TOPO TA vector - (reply: 3)
1487. nondenaturing PAGE to separate dna - (reply: 4)
1488. why sometimes big or small colonies? - (reply: 16)
1489. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1490. wrong bands after T-vector cloning - (reply: 3)
1491. urgent help regarding wanner method trouble shooting - (reply: 2)
1492. two step digestion - (reply: 6)
1493. Storage of invitrogen top10 cells - (reply: 11)
1494. Self-Ligation of digested vector - (reply: 2)
1495. Help with cloning - (reply: 6)
1496. Ligation issue: Does vector size matter? - (reply: 10)
1497. Help for T-A cloning - (reply: 15)
1498. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1499. Some colonies, but not enough... - (reply: 7)
1500. transformation problem - (reply: 3)
1472. insert is missing after TA cloning - TA cloning (reply: 6)
1473. glycerol stock - shelf life (reply: 5)
1474. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1475. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1476. cloning problem - molecular cloning (reply: 1)
1477. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1478. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1479. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1480. choosing restriction sites - (reply: 5)
1481. Tag insertion - (reply: 5)
1482. Transduction - (reply: 3)
1483. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1484. my colonies don't have the insert, how is this possible? - (reply: 7)
1485. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1486. PCR problem from transformed TOPO TA vector - (reply: 3)
1487. nondenaturing PAGE to separate dna - (reply: 4)
1488. why sometimes big or small colonies? - (reply: 16)
1489. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1490. wrong bands after T-vector cloning - (reply: 3)
1491. urgent help regarding wanner method trouble shooting - (reply: 2)
1492. two step digestion - (reply: 6)
1493. Storage of invitrogen top10 cells - (reply: 11)
1494. Self-Ligation of digested vector - (reply: 2)
1495. Help with cloning - (reply: 6)
1496. Ligation issue: Does vector size matter? - (reply: 10)
1497. Help for T-A cloning - (reply: 15)
1498. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1499. Some colonies, but not enough... - (reply: 7)
1500. transformation problem - (reply: 3)