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Top : New Forum Archives (2009-): : Molecular-Cloning
1471. cloning problem - molecular cloning (reply: 1)
1472. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1473. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1474. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1475. choosing restriction sites - (reply: 5)
1476. Tag insertion - (reply: 5)
1477. Transduction - (reply: 3)
1478. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1479. my colonies don't have the insert, how is this possible? - (reply: 7)
1480. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1481. PCR problem from transformed TOPO TA vector - (reply: 3)
1482. nondenaturing PAGE to separate dna - (reply: 4)
1483. why sometimes big or small colonies? - (reply: 16)
1484. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1485. wrong bands after T-vector cloning - (reply: 3)
1486. urgent help regarding wanner method trouble shooting - (reply: 2)
1487. two step digestion - (reply: 6)
1488. Storage of invitrogen top10 cells - (reply: 11)
1489. Self-Ligation of digested vector - (reply: 2)
1490. Help with cloning - (reply: 6)
1491. Ligation issue: Does vector size matter? - (reply: 10)
1492. Help for T-A cloning - (reply: 15)
1493. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1494. Some colonies, but not enough... - (reply: 7)
1495. transformation problem - (reply: 3)
1496. Plasmid sequence - (reply: 1)
1497. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1498. help wih the ligation - (reply: 7)
1499. PCR screening of transformed bacterial colony - (reply: 6)
1500. No colony for oligo cloning - (reply: 9)