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Top : New Forum Archives (2009-): : Molecular-Cloning
1471. nondenaturing PAGE to separate dna - (reply: 4)
1472. why sometimes big or small colonies? - (reply: 16)
1473. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1474. wrong bands after T-vector cloning - (reply: 3)
1475. urgent help regarding wanner method trouble shooting - (reply: 2)
1476. two step digestion - (reply: 6)
1477. Storage of invitrogen top10 cells - (reply: 11)
1478. Self-Ligation of digested vector - (reply: 2)
1479. Help with cloning - (reply: 6)
1480. Ligation issue: Does vector size matter? - (reply: 10)
1481. Help for T-A cloning - (reply: 15)
1482. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1483. Some colonies, but not enough... - (reply: 7)
1484. transformation problem - (reply: 3)
1485. Plasmid sequence - (reply: 1)
1486. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
1487. help wih the ligation - (reply: 7)
1488. PCR screening of transformed bacterial colony - (reply: 6)
1489. No colony for oligo cloning - (reply: 9)
1490. cloning - affect on c-terminal (reply: 1)
1491. Growing of TOP10 chemically competent cells - (reply: 4)
1492. Stable Cell Selection with Neomycin - (reply: 4)
1493. lpzzzzz that is so important to my PhD. - (reply: 1)
1494. need guidence for cdna library - screen library (reply: 5)
1495. No product after restriction digestion - (reply: 26)
1496. protein overexpression question. - (reply: 4)
1497. Basic doubt on restriction digestion - (reply: 2)
1498. Transform staph? - (reply: 2)
1499. help for cDNA synthesis - plus RE problem. (reply: 3)
1500. adjacent restriction sites - (reply: 6)