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Top : New Forum Archives (2009-): : Molecular-Cloning
781. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
782. Three-way ligation in two steps? - (reply: 3)
783. How does circular, uncut pGEM-T migrate? - (reply: 2)
784. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
785. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
786. Cloning App Released from Life Technologies - (reply: 2)
787. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
788. Dissolve IPTG in EtOH - (reply: 4)
789. problem with transient transfection - (reply: 6)
790. Recombinant construct looks smaller than empty vector - (reply: 3)
791. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
792. hygromycin concentration for e-coli selection - (reply: 1)
793. orientation of promoter into viral construct - (reply: 1)
794. problems with PCR confirmation of insert . HELP ! - (reply: 1)
795. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
796. Loss of promoter during ligation - (reply: 2)
797. Enzyme restriction - (reply: 8)
798. Colony PCR Problem!!!! - (reply: 5)
799. Removing unwanted sequence from pUC19 - (reply: 4)
800. transformation fails, bacteria grows in control plate - (reply: 6)
801. competentce cell, cloning - (reply: 2)
802. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
803. co-transformation - (reply: 3)
804. pcr clean-up fail? - (reply: 1)
805. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
806. sequential cloning of multiple PCR products - how to do? (reply: 3)
807. Cloning large gene - (reply: 3)
808. Glycosilated peptide's cDNA cloning - (reply: 3)
809. pWM91 cloning problem - (reply: 4)
810. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)