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Top : New Forum Archives (2009-): : Molecular-Cloning
781. Cloning App Released from Life Technologies - (reply: 2)
782. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
783. Dissolve IPTG in EtOH - (reply: 4)
784. problem with transient transfection - (reply: 6)
785. Recombinant construct looks smaller than empty vector - (reply: 3)
786. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
787. hygromycin concentration for e-coli selection - (reply: 1)
788. orientation of promoter into viral construct - (reply: 1)
789. problems with PCR confirmation of insert . HELP ! - (reply: 1)
790. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
791. Loss of promoter during ligation - (reply: 2)
792. Enzyme restriction - (reply: 8)
793. Colony PCR Problem!!!! - (reply: 5)
794. Removing unwanted sequence from pUC19 - (reply: 4)
795. transformation fails, bacteria grows in control plate - (reply: 6)
796. competentce cell, cloning - (reply: 2)
797. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
798. co-transformation - (reply: 3)
799. pcr clean-up fail? - (reply: 1)
800. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
801. sequential cloning of multiple PCR products - how to do? (reply: 3)
802. Cloning large gene - (reply: 3)
803. Glycosilated peptide's cDNA cloning - (reply: 3)
804. pWM91 cloning problem - (reply: 4)
805. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
806. Transformation - Transformation (reply: 1)
807. how to add 3XHA to the N-terminal of a protein - (reply: 5)
808. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
809. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
810. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)