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Top : New Forum Archives (2009-): : Molecular-Cloning
781. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
782. Cloning App Released from Life Technologies - (reply: 2)
783. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
784. Dissolve IPTG in EtOH - (reply: 4)
785. problem with transient transfection - (reply: 6)
786. Recombinant construct looks smaller than empty vector - (reply: 3)
787. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
788. hygromycin concentration for e-coli selection - (reply: 1)
789. orientation of promoter into viral construct - (reply: 1)
790. problems with PCR confirmation of insert . HELP ! - (reply: 1)
791. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
792. Loss of promoter during ligation - (reply: 2)
793. Enzyme restriction - (reply: 8)
794. Colony PCR Problem!!!! - (reply: 5)
795. Removing unwanted sequence from pUC19 - (reply: 4)
796. transformation fails, bacteria grows in control plate - (reply: 6)
797. competentce cell, cloning - (reply: 2)
798. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
799. co-transformation - (reply: 3)
800. pcr clean-up fail? - (reply: 1)
801. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
802. sequential cloning of multiple PCR products - how to do? (reply: 3)
803. Cloning large gene - (reply: 3)
804. Glycosilated peptide's cDNA cloning - (reply: 3)
805. pWM91 cloning problem - (reply: 4)
806. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
807. Transformation - Transformation (reply: 1)
808. how to add 3XHA to the N-terminal of a protein - (reply: 5)
809. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
810. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)