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Top : New Forum Archives (2009-): : Molecular-Cloning
781. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
782. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
783. Cloning App Released from Life Technologies - (reply: 2)
784. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
785. Dissolve IPTG in EtOH - (reply: 4)
786. problem with transient transfection - (reply: 6)
787. Recombinant construct looks smaller than empty vector - (reply: 3)
788. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
789. hygromycin concentration for e-coli selection - (reply: 1)
790. orientation of promoter into viral construct - (reply: 1)
791. problems with PCR confirmation of insert . HELP ! - (reply: 1)
792. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
793. Loss of promoter during ligation - (reply: 2)
794. Enzyme restriction - (reply: 8)
795. Colony PCR Problem!!!! - (reply: 5)
796. Removing unwanted sequence from pUC19 - (reply: 4)
797. transformation fails, bacteria grows in control plate - (reply: 6)
798. competentce cell, cloning - (reply: 2)
799. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
800. co-transformation - (reply: 3)
801. pcr clean-up fail? - (reply: 1)
802. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
803. sequential cloning of multiple PCR products - how to do? (reply: 3)
804. Cloning large gene - (reply: 3)
805. Glycosilated peptide's cDNA cloning - (reply: 3)
806. pWM91 cloning problem - (reply: 4)
807. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
808. Transformation - Transformation (reply: 1)
809. how to add 3XHA to the N-terminal of a protein - (reply: 5)
810. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)