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Top : New Forum Archives (2009-): : Molecular-Cloning
781. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
782. ligation of pcr product into expression vector - (reply: 4)
783. amp plates containing xgal/iptg with expression vector - (reply: 6)
784. Strange Vector Problems - (reply: 3)
785. white colonies - (reply: 2)
786. how to design a kozak sequence in front of the start codon - (reply: 1)
787. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
788. XhoI digestion - (reply: 12)
789. Lenti vector ligation - (reply: 2)
790. Cloning from a pGEMT Easy vector - (reply: 3)
791. Distance between RE sites for double digestion - (reply: 5)
792. Plasmid compatible with pBAD24 - (reply: 1)
793. I cant get colony - (reply: 3)
794. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
795. Vector with inducible/repressible system - (reply: 1)
796. cloning problem - (reply: 9)
797. Cloning genes into plant vector - (reply: 1)
798. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
799. Three-way ligation in two steps? - (reply: 3)
800. How does circular, uncut pGEM-T migrate? - (reply: 2)
801. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
802. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
803. Cloning App Released from Life Technologies - (reply: 2)
804. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
805. Dissolve IPTG in EtOH - (reply: 4)
806. problem with transient transfection - (reply: 6)
807. Recombinant construct looks smaller than empty vector - (reply: 3)
808. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
809. hygromycin concentration for e-coli selection - (reply: 1)
810. orientation of promoter into viral construct - (reply: 1)