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Top : New Forum Archives (2009-): : Molecular-Cloning
781. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
782. MDA-MB-231 cell transfection - (reply: 4)
783. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
784. Break Cell Clumps - prepare single cells for injections (reply: 1)
785. 126pb insert clonnig/ digestion issues - (reply: 3)
786. cloning problem... - (reply: 5)
787. yeast glycerol stock - (reply: 2)
788. yeast transformation - (reply: 1)
789. Problems with ligation and transformation - (reply: 2)
790. very small insert and a very ling vector - (reply: 1)
791. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
792. cDNA clone - (reply: 1)
793. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
794. why these guys use T7 instead of CMV? - for transfection (reply: 2)
795. Electroporation - no colonies (reply: 1)
796. enzymes inactivation - (reply: 3)
797. help: why plasmid yield dropped ever since the first culture - (reply: 2)
798. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
799. miRNA cloning - (reply: 2)
800. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
801. Single-digested vector shifts downward on gel - (reply: 1)
802. problem in digestion - (reply: 1)
803. Cloning does not work - (reply: 4)
804. Small Colonies After Transformation - (reply: 5)
805. Ligation and transformation problem - (reply: 2)
806. Agrobacterium EHA101 competent cells - (reply: 1)
807. restriction digestion against colony PCR - (reply: 1)
808. BAC for transgenic mice - (reply: 6)
809. Tag proteins for CO-IP and confocal - (reply: 1)
810. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)