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Top : New Forum Archives (2009-): : Molecular-Cloning
781. very small insert and a very ling vector - (reply: 1)
782. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
783. cDNA clone - (reply: 1)
784. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
785. why these guys use T7 instead of CMV? - for transfection (reply: 2)
786. Electroporation - no colonies (reply: 1)
787. enzymes inactivation - (reply: 3)
788. help: why plasmid yield dropped ever since the first culture - (reply: 2)
789. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
790. miRNA cloning - (reply: 2)
791. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
792. Single-digested vector shifts downward on gel - (reply: 1)
793. problem in digestion - (reply: 1)
794. Cloning does not work - (reply: 4)
795. Small Colonies After Transformation - (reply: 5)
796. Ligation and transformation problem - (reply: 2)
797. Agrobacterium EHA101 competent cells - (reply: 1)
798. restriction digestion against colony PCR - (reply: 1)
799. BAC for transgenic mice - (reply: 6)
800. Tag proteins for CO-IP and confocal - (reply: 1)
801. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
802. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
803. how long for a double digestion - (reply: 2)
804. Problems with Site-Directed Mutagenesis - (reply: 2)
805. blunt and sticky end clonning temperature - (reply: 2)
806. Most common mistakes - What mistakes are made during cloning? (reply: 7)
807. Luciferase Assay Stim. Concentration? - (reply: 1)
808. Cloning direction / orientation? - (reply: 2)
809. Question about plasmid midiprep - (reply: 1)
810. Does a polyA sequence need to be put in-frame? - (reply: 1)