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Top : New Forum Archives (2009-): : Molecular-Cloning
781. white colonies - (reply: 2)
782. how to design a kozak sequence in front of the start codon - (reply: 1)
783. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
784. XhoI digestion - (reply: 12)
785. Lenti vector ligation - (reply: 2)
786. Cloning from a pGEMT Easy vector - (reply: 3)
787. Distance between RE sites for double digestion - (reply: 5)
788. Plasmid compatible with pBAD24 - (reply: 1)
789. I cant get colony - (reply: 3)
790. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
791. Vector with inducible/repressible system - (reply: 1)
792. cloning problem - (reply: 9)
793. Cloning genes into plant vector - (reply: 1)
794. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
795. Three-way ligation in two steps? - (reply: 3)
796. How does circular, uncut pGEM-T migrate? - (reply: 2)
797. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
798. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
799. Cloning App Released from Life Technologies - (reply: 2)
800. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
801. Dissolve IPTG in EtOH - (reply: 4)
802. problem with transient transfection - (reply: 6)
803. Recombinant construct looks smaller than empty vector - (reply: 3)
804. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
805. hygromycin concentration for e-coli selection - (reply: 1)
806. orientation of promoter into viral construct - (reply: 1)
807. problems with PCR confirmation of insert . HELP ! - (reply: 1)
808. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
809. Loss of promoter during ligation - (reply: 2)
810. Enzyme restriction - (reply: 8)