Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
781. Tag proteins for CO-IP and confocal - (reply: 1)
782. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
783. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
784. how long for a double digestion - (reply: 2)
785. Problems with Site-Directed Mutagenesis - (reply: 2)
786. blunt and sticky end clonning temperature - (reply: 2)
787. Most common mistakes - What mistakes are made during cloning? (reply: 7)
788. Luciferase Assay Stim. Concentration? - (reply: 1)
789. Cloning direction / orientation? - (reply: 2)
790. Question about plasmid midiprep - (reply: 1)
791. Does a polyA sequence need to be put in-frame? - (reply: 1)
792. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
793. Problem on growing up bugs - (reply: 8)
794. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
795. pSIM in BL21(DE3) - (reply: 2)
796. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
797. Weird problem in invitromutagenesis - (reply: 3)
798. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
799. RE digestion - (reply: 4)
800. HELP!!! - (reply: 3)
801. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
802. Cloning of ITS region - (reply: 6)
803. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
804. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
805. SmaI blunt end ligation problem... - (reply: 3)
806. AAV construct? - (reply: 1)
807. No bands on agarose gel after restriction digestion. - (reply: 10)
808. inverse pcr - (reply: 2)
809. Viral cDNA Library - (reply: 3)
810. metylated cloning vector - restriction digest of the insert (reply: 2)