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Top : New Forum Archives (2009-): : Molecular-Cloning
781. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
782. Vector with inducible/repressible system - (reply: 1)
783. cloning problem - (reply: 9)
784. Cloning genes into plant vector - (reply: 1)
785. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
786. Three-way ligation in two steps? - (reply: 3)
787. How does circular, uncut pGEM-T migrate? - (reply: 2)
788. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
789. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
790. Cloning App Released from Life Technologies - (reply: 2)
791. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
792. Dissolve IPTG in EtOH - (reply: 4)
793. problem with transient transfection - (reply: 6)
794. Recombinant construct looks smaller than empty vector - (reply: 3)
795. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
796. hygromycin concentration for e-coli selection - (reply: 1)
797. orientation of promoter into viral construct - (reply: 1)
798. problems with PCR confirmation of insert . HELP ! - (reply: 1)
799. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
800. Loss of promoter during ligation - (reply: 2)
801. Enzyme restriction - (reply: 8)
802. Colony PCR Problem!!!! - (reply: 5)
803. Removing unwanted sequence from pUC19 - (reply: 4)
804. transformation fails, bacteria grows in control plate - (reply: 6)
805. competentce cell, cloning - (reply: 2)
806. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
807. co-transformation - (reply: 3)
808. pcr clean-up fail? - (reply: 1)
809. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
810. sequential cloning of multiple PCR products - how to do? (reply: 3)