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Top : New Forum Archives (2009-): : Molecular-Cloning
781. Plasmid compatible with pBAD24 - (reply: 1)
782. I cant get colony - (reply: 3)
783. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
784. Vector with inducible/repressible system - (reply: 1)
785. cloning problem - (reply: 9)
786. Cloning genes into plant vector - (reply: 1)
787. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
788. Three-way ligation in two steps? - (reply: 3)
789. How does circular, uncut pGEM-T migrate? - (reply: 2)
790. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
791. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
792. Cloning App Released from Life Technologies - (reply: 2)
793. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
794. Dissolve IPTG in EtOH - (reply: 4)
795. problem with transient transfection - (reply: 6)
796. Recombinant construct looks smaller than empty vector - (reply: 3)
797. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
798. hygromycin concentration for e-coli selection - (reply: 1)
799. orientation of promoter into viral construct - (reply: 1)
800. problems with PCR confirmation of insert . HELP ! - (reply: 1)
801. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
802. Loss of promoter during ligation - (reply: 2)
803. Enzyme restriction - (reply: 8)
804. Colony PCR Problem!!!! - (reply: 5)
805. Removing unwanted sequence from pUC19 - (reply: 4)
806. transformation fails, bacteria grows in control plate - (reply: 6)
807. competentce cell, cloning - (reply: 2)
808. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
809. co-transformation - (reply: 3)
810. pcr clean-up fail? - (reply: 1)