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781. Plasmid size problems! - (reply: 3)
782. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
783. ligation problem - (reply: 6)
784. Failed Ligation - (reply: 2)
785. cloning of GroEL - (reply: 3)
786. Ligation problem - (reply: 9)
787. Problems with High Background with Gateway Cloning - (reply: 1)
788. Mutagenesis troubles - (reply: 2)
789. ligation/transformation failed - (reply: 7)
790. Transfecting primary cells with plasmid - (reply: 1)
791. Extra bands after QIAGEN spin miniprep - (reply: 3)
792. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
793. The use of hygromycin - (reply: 2)
794. QUESTION about cloning - (reply: 5)
795. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
796. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
797. Ligation screening - (reply: 4)
798. Double digestion and ligation problem - (reply: 1)
799. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
800. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
801. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
802. CMV promoter - (reply: 2)
803. insert in the reverse orientation after transformation - (reply: 5)
804. cDNA as PCR template - (reply: 1)
805. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
806. T4 ligase buffer - (reply: 4)
807. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
808. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
809. ligation problems - no colonies at the end!!!!! (reply: 4)
810. Good stable mammalian promoter - (reply: 1)
782. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
783. ligation problem - (reply: 6)
784. Failed Ligation - (reply: 2)
785. cloning of GroEL - (reply: 3)
786. Ligation problem - (reply: 9)
787. Problems with High Background with Gateway Cloning - (reply: 1)
788. Mutagenesis troubles - (reply: 2)
789. ligation/transformation failed - (reply: 7)
790. Transfecting primary cells with plasmid - (reply: 1)
791. Extra bands after QIAGEN spin miniprep - (reply: 3)
792. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
793. The use of hygromycin - (reply: 2)
794. QUESTION about cloning - (reply: 5)
795. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
796. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
797. Ligation screening - (reply: 4)
798. Double digestion and ligation problem - (reply: 1)
799. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
800. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
801. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
802. CMV promoter - (reply: 2)
803. insert in the reverse orientation after transformation - (reply: 5)
804. cDNA as PCR template - (reply: 1)
805. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
806. T4 ligase buffer - (reply: 4)
807. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
808. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
809. ligation problems - no colonies at the end!!!!! (reply: 4)
810. Good stable mammalian promoter - (reply: 1)