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Top : New Forum Archives (2009-): : Molecular-Cloning
781. PCR clonning - (reply: 3)
782. 2 bands in blue colony PCR - (reply: 5)
783. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
784. Introduction to vector development - Basics on vectors types and construction (reply: 1)
785. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
786. Targeted mutagenesis using a linear fragment - (reply: 1)
787. Transformation & Minimal Plates - (reply: 5)
788. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
789. Subcloning Problem - (reply: 3)
790. Restriction Enzyme sites in my genes - (reply: 1)
791. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
792. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
793. In-Fusion (Clontech) Reaction buffer - (reply: 1)
794. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
795. MDA-MB-231 cell transfection - (reply: 4)
796. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
797. Break Cell Clumps - prepare single cells for injections (reply: 1)
798. 126pb insert clonnig/ digestion issues - (reply: 3)
799. cloning problem... - (reply: 5)
800. yeast glycerol stock - (reply: 2)
801. yeast transformation - (reply: 1)
802. Problems with ligation and transformation - (reply: 2)
803. very small insert and a very ling vector - (reply: 1)
804. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
805. cDNA clone - (reply: 1)
806. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
807. why these guys use T7 instead of CMV? - for transfection (reply: 2)
808. Electroporation - no colonies (reply: 1)
809. enzymes inactivation - (reply: 3)
810. help: why plasmid yield dropped ever since the first culture - (reply: 2)