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Top : New Forum Archives (2009-): : Molecular-Cloning
721. making recombinant protein - (reply: 2)
722. Confirming insert???? - (reply: 1)
723. how to make a recombinant protein with signal peptide - (reply: 1)
724. How to add restriction enzyme site into the DNA fragment - (reply: 3)
725. Issues with ligation - (reply: 2)
726. Ligation of double insert into pET31 failing - (reply: 1)
727. Homemade MiniPreps for Plasmid Purification - (reply: 1)
728. Agar has dried out during cultivation - (reply: 1)
729. Gateway cloning vector compatibility - (reply: 2)
730. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
731. Cloning of IL8-promoter - (reply: 3)
732. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
733. Template DNA (plasmid) concentration for PCR - (reply: 4)
734. Ligation of 16kb PCR product - (reply: 5)
735. stable transfection - (reply: 1)
736. How to choose the right plasmids for certain types of cell? - (reply: 1)
737. Ligation needed after SDM with PfuX? - (reply: 3)
738. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
739. Cloning promoter (overlap with another gene!) - (reply: 3)
740. bacterial transfeomation - (reply: 2)
741. Loss of insert? - (reply: 1)
742. Sequencing trouble - (reply: 7)
743. Platinum-A cell trasnfection toruble - (reply: 1)
744. Cloning a promoter - (reply: 2)
745. pcDNA 3.1 His Expression - (reply: 2)
746. Cloning two fragments into a vector - (reply: 3)
747. confirmation of insert in to vector - (reply: 3)
748. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
749. Question about Molar ratio - (reply: 4)
750. Ligation problem - (reply: 16)