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Top : New Forum Archives (2009-): : Molecular-Cloning
721. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
722. ligation and transformation problem - (reply: 1)
723. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
724. Mini prep of frozen bacteria cells - (reply: 2)
725. PCR cloning problem - could not get colony (reply: 4)
726. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
727. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
728. PCR clonning - (reply: 3)
729. 2 bands in blue colony PCR - (reply: 5)
730. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
731. Introduction to vector development - Basics on vectors types and construction (reply: 1)
732. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
733. Targeted mutagenesis using a linear fragment - (reply: 1)
734. Transformation & Minimal Plates - (reply: 5)
735. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
736. Subcloning Problem - (reply: 3)
737. Restriction Enzyme sites in my genes - (reply: 1)
738. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
739. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
740. In-Fusion (Clontech) Reaction buffer - (reply: 1)
741. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
742. MDA-MB-231 cell transfection - (reply: 4)
743. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
744. Break Cell Clumps - prepare single cells for injections (reply: 1)
745. 126pb insert clonnig/ digestion issues - (reply: 3)
746. cloning problem... - (reply: 5)
747. yeast glycerol stock - (reply: 2)
748. yeast transformation - (reply: 1)
749. Problems with ligation and transformation - (reply: 2)
750. very small insert and a very ling vector - (reply: 1)