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Top : New Forum Archives (2009-): : Molecular-Cloning
721. What does vortexing do in transformation? - (reply: 1)
722. failure in miniprep - (reply: 1)
723. Host cells for transformation - (reply: 3)
724. making recombinant protein - (reply: 2)
725. Confirming insert???? - (reply: 1)
726. how to make a recombinant protein with signal peptide - (reply: 1)
727. How to add restriction enzyme site into the DNA fragment - (reply: 3)
728. Issues with ligation - (reply: 2)
729. Ligation of double insert into pET31 failing - (reply: 1)
730. Homemade MiniPreps for Plasmid Purification - (reply: 1)
731. Agar has dried out during cultivation - (reply: 1)
732. Gateway cloning vector compatibility - (reply: 2)
733. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
734. Cloning of IL8-promoter - (reply: 3)
735. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
736. Template DNA (plasmid) concentration for PCR - (reply: 4)
737. Ligation of 16kb PCR product - (reply: 5)
738. stable transfection - (reply: 1)
739. How to choose the right plasmids for certain types of cell? - (reply: 1)
740. Ligation needed after SDM with PfuX? - (reply: 3)
741. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
742. Cloning promoter (overlap with another gene!) - (reply: 3)
743. bacterial transfeomation - (reply: 2)
744. Loss of insert? - (reply: 1)
745. Sequencing trouble - (reply: 7)
746. Platinum-A cell trasnfection toruble - (reply: 1)
747. Cloning a promoter - (reply: 2)
748. pcDNA 3.1 His Expression - (reply: 2)
749. Cloning two fragments into a vector - (reply: 3)
750. confirmation of insert in to vector - (reply: 3)