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Top : New Forum Archives (2009-): : Molecular-Cloning
721. bacterial transformation problem - (reply: 2)
722. Transformation - (reply: 3)
723. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
724. What does vortexing do in transformation? - (reply: 1)
725. failure in miniprep - (reply: 1)
726. Host cells for transformation - (reply: 3)
727. making recombinant protein - (reply: 2)
728. Confirming insert???? - (reply: 1)
729. how to make a recombinant protein with signal peptide - (reply: 1)
730. How to add restriction enzyme site into the DNA fragment - (reply: 3)
731. Issues with ligation - (reply: 2)
732. Ligation of double insert into pET31 failing - (reply: 1)
733. Homemade MiniPreps for Plasmid Purification - (reply: 1)
734. Agar has dried out during cultivation - (reply: 1)
735. Gateway cloning vector compatibility - (reply: 2)
736. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
737. Cloning of IL8-promoter - (reply: 3)
738. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
739. Template DNA (plasmid) concentration for PCR - (reply: 4)
740. Ligation of 16kb PCR product - (reply: 5)
741. stable transfection - (reply: 1)
742. How to choose the right plasmids for certain types of cell? - (reply: 1)
743. Ligation needed after SDM with PfuX? - (reply: 3)
744. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
745. Cloning promoter (overlap with another gene!) - (reply: 3)
746. bacterial transfeomation - (reply: 2)
747. Loss of insert? - (reply: 1)
748. Sequencing trouble - (reply: 7)
749. Platinum-A cell trasnfection toruble - (reply: 1)
750. Cloning a promoter - (reply: 2)