Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
721. Subcloning Problem - (reply: 3)
722. Restriction Enzyme sites in my genes - (reply: 1)
723. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
724. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
725. In-Fusion (Clontech) Reaction buffer - (reply: 1)
726. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
727. MDA-MB-231 cell transfection - (reply: 4)
728. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
729. Break Cell Clumps - prepare single cells for injections (reply: 1)
730. 126pb insert clonnig/ digestion issues - (reply: 3)
731. cloning problem... - (reply: 5)
732. yeast glycerol stock - (reply: 2)
733. yeast transformation - (reply: 1)
734. Problems with ligation and transformation - (reply: 2)
735. very small insert and a very ling vector - (reply: 1)
736. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
737. cDNA clone - (reply: 1)
738. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
739. why these guys use T7 instead of CMV? - for transfection (reply: 2)
740. Electroporation - no colonies (reply: 1)
741. enzymes inactivation - (reply: 3)
742. help: why plasmid yield dropped ever since the first culture - (reply: 2)
743. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
744. miRNA cloning - (reply: 2)
745. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
746. Single-digested vector shifts downward on gel - (reply: 1)
747. problem in digestion - (reply: 1)
748. Cloning does not work - (reply: 4)
749. Small Colonies After Transformation - (reply: 5)
750. Ligation and transformation problem - (reply: 2)