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Top : New Forum Archives (2009-): : Molecular-Cloning
721. Fixing SNPs after cloning - (reply: 5)
722. Electroporated plasmid loses the insert - (reply: 8)
723. Recombination due to overselective plate? - (reply: 11)
724. Help with ligation - (reply: 2)
725. emty vector cloning - (reply: 3)
726. RE site on complimentary strand of plasmid? - (reply: 1)
727. LENTIVIRUS transfection efficiency - (reply: 1)
728. Double digest after cloning doesn't give gene - (reply: 6)
729. Blunt end PCR product ligation - (reply: 1)
730. Screening for clones - (reply: 2)
731. Plasmid isolation Qiagen - (reply: 2)
732. Restriction Site Destruction - (reply: 1)
733. The copy number of pPICZ alpha A - (reply: 6)
734. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
735. need help with subcloning !! - (reply: 2)
736. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
737. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
738. cdna library. - (reply: 2)
739. Help in RE digestion of vector - (reply: 5)
740. bacterial transformation problem - (reply: 2)
741. Transformation - (reply: 3)
742. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
743. What does vortexing do in transformation? - (reply: 1)
744. failure in miniprep - (reply: 1)
745. Host cells for transformation - (reply: 3)
746. making recombinant protein - (reply: 2)
747. Confirming insert???? - (reply: 1)
748. how to make a recombinant protein with signal peptide - (reply: 1)
749. How to add restriction enzyme site into the DNA fragment - (reply: 3)
750. Issues with ligation - (reply: 2)