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Top : New Forum Archives (2009-): : Molecular-Cloning
721. cdna library. - (reply: 2)
722. Help in RE digestion of vector - (reply: 5)
723. bacterial transformation problem - (reply: 2)
724. Transformation - (reply: 3)
725. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
726. What does vortexing do in transformation? - (reply: 1)
727. failure in miniprep - (reply: 1)
728. Host cells for transformation - (reply: 3)
729. making recombinant protein - (reply: 2)
730. Confirming insert???? - (reply: 1)
731. how to make a recombinant protein with signal peptide - (reply: 1)
732. How to add restriction enzyme site into the DNA fragment - (reply: 3)
733. Issues with ligation - (reply: 2)
734. Ligation of double insert into pET31 failing - (reply: 1)
735. Homemade MiniPreps for Plasmid Purification - (reply: 1)
736. Agar has dried out during cultivation - (reply: 1)
737. Gateway cloning vector compatibility - (reply: 2)
738. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
739. Cloning of IL8-promoter - (reply: 3)
740. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
741. Template DNA (plasmid) concentration for PCR - (reply: 4)
742. Ligation of 16kb PCR product - (reply: 5)
743. stable transfection - (reply: 1)
744. How to choose the right plasmids for certain types of cell? - (reply: 1)
745. Ligation needed after SDM with PfuX? - (reply: 3)
746. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
747. Cloning promoter (overlap with another gene!) - (reply: 3)
748. bacterial transfeomation - (reply: 2)
749. Loss of insert? - (reply: 1)
750. Sequencing trouble - (reply: 7)