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Top : New Forum Archives (2009-): : Molecular-Cloning
721. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
722. Cloning of IL8-promoter - (reply: 3)
723. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
724. Template DNA (plasmid) concentration for PCR - (reply: 4)
725. Ligation of 16kb PCR product - (reply: 5)
726. stable transfection - (reply: 1)
727. How to choose the right plasmids for certain types of cell? - (reply: 1)
728. Ligation needed after SDM with PfuX? - (reply: 3)
729. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
730. Cloning promoter (overlap with another gene!) - (reply: 3)
731. bacterial transfeomation - (reply: 2)
732. Loss of insert? - (reply: 1)
733. Sequencing trouble - (reply: 7)
734. Platinum-A cell trasnfection toruble - (reply: 1)
735. Cloning a promoter - (reply: 2)
736. pcDNA 3.1 His Expression - (reply: 2)
737. Cloning two fragments into a vector - (reply: 3)
738. confirmation of insert in to vector - (reply: 3)
739. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
740. Question about Molar ratio - (reply: 4)
741. Ligation problem - (reply: 16)
742. double digestion with sac1, hindIII - (reply: 3)
743. self-ligation Anneled oligos - (reply: 7)
744. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
745. Looking for a GFP plasmid (non-amp) - (reply: 3)
746. clonning problem (recombined by-products) - (reply: 8)
747. Genecloning three types of molecules? - (reply: 11)
748. help in digesting DNA fragment using restriction enzyme - (reply: 2)
749. Tansformation fails ,how can i make it ? - (reply: 3)
750. pelB leader - how to use - (reply: 1)