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Top : New Forum Archives (2009-): : Molecular-Cloning
721. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
722. cdna library. - (reply: 2)
723. Help in RE digestion of vector - (reply: 5)
724. bacterial transformation problem - (reply: 2)
725. Transformation - (reply: 3)
726. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
727. What does vortexing do in transformation? - (reply: 1)
728. failure in miniprep - (reply: 1)
729. Host cells for transformation - (reply: 3)
730. making recombinant protein - (reply: 2)
731. Confirming insert???? - (reply: 1)
732. how to make a recombinant protein with signal peptide - (reply: 1)
733. How to add restriction enzyme site into the DNA fragment - (reply: 3)
734. Issues with ligation - (reply: 2)
735. Ligation of double insert into pET31 failing - (reply: 1)
736. Homemade MiniPreps for Plasmid Purification - (reply: 1)
737. Agar has dried out during cultivation - (reply: 1)
738. Gateway cloning vector compatibility - (reply: 2)
739. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
740. Cloning of IL8-promoter - (reply: 3)
741. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
742. Template DNA (plasmid) concentration for PCR - (reply: 4)
743. Ligation of 16kb PCR product - (reply: 5)
744. stable transfection - (reply: 1)
745. How to choose the right plasmids for certain types of cell? - (reply: 1)
746. Ligation needed after SDM with PfuX? - (reply: 3)
747. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
748. Cloning promoter (overlap with another gene!) - (reply: 3)
749. bacterial transfeomation - (reply: 2)
750. Loss of insert? - (reply: 1)