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Top : New Forum Archives (2009-): : Molecular-Cloning
721. Ligation needed after SDM with PfuX? - (reply: 3)
722. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
723. Cloning promoter (overlap with another gene!) - (reply: 3)
724. bacterial transfeomation - (reply: 2)
725. Loss of insert? - (reply: 1)
726. Sequencing trouble - (reply: 7)
727. Platinum-A cell trasnfection toruble - (reply: 1)
728. Cloning a promoter - (reply: 2)
729. pcDNA 3.1 His Expression - (reply: 2)
730. Cloning two fragments into a vector - (reply: 3)
731. confirmation of insert in to vector - (reply: 3)
732. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
733. Question about Molar ratio - (reply: 4)
734. Ligation problem - (reply: 16)
735. double digestion with sac1, hindIII - (reply: 3)
736. self-ligation Anneled oligos - (reply: 7)
737. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
738. Looking for a GFP plasmid (non-amp) - (reply: 3)
739. clonning problem (recombined by-products) - (reply: 8)
740. Genecloning three types of molecules? - (reply: 11)
741. help in digesting DNA fragment using restriction enzyme - (reply: 2)
742. Tansformation fails ,how can i make it ? - (reply: 3)
743. pelB leader - how to use - (reply: 1)
744. Transformation bacteria heat chock and incubation? - (reply: 2)
745. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
746. ligation of pcr product into expression vector - (reply: 4)
747. amp plates containing xgal/iptg with expression vector - (reply: 6)
748. Strange Vector Problems - (reply: 3)
749. white colonies - (reply: 2)
750. how to design a kozak sequence in front of the start codon - (reply: 1)