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Top : New Forum Archives (2009-): : Molecular-Cloning
721. LENTIVIRUS transfection efficiency - (reply: 1)
722. Double digest after cloning doesn't give gene - (reply: 6)
723. Blunt end PCR product ligation - (reply: 1)
724. Screening for clones - (reply: 2)
725. Plasmid isolation Qiagen - (reply: 2)
726. Restriction Site Destruction - (reply: 1)
727. The copy number of pPICZ alpha A - (reply: 6)
728. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
729. need help with subcloning !! - (reply: 2)
730. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
731. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
732. cdna library. - (reply: 2)
733. Help in RE digestion of vector - (reply: 5)
734. bacterial transformation problem - (reply: 2)
735. Transformation - (reply: 3)
736. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
737. What does vortexing do in transformation? - (reply: 1)
738. failure in miniprep - (reply: 1)
739. Host cells for transformation - (reply: 3)
740. making recombinant protein - (reply: 2)
741. Confirming insert???? - (reply: 1)
742. how to make a recombinant protein with signal peptide - (reply: 1)
743. How to add restriction enzyme site into the DNA fragment - (reply: 3)
744. Issues with ligation - (reply: 2)
745. Ligation of double insert into pET31 failing - (reply: 1)
746. Homemade MiniPreps for Plasmid Purification - (reply: 1)
747. Agar has dried out during cultivation - (reply: 1)
748. Gateway cloning vector compatibility - (reply: 2)
749. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
750. Cloning of IL8-promoter - (reply: 3)