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Top : New Forum Archives (2009-): : Molecular-Cloning
721. Confirming insert???? - (reply: 1)
722. how to make a recombinant protein with signal peptide - (reply: 1)
723. How to add restriction enzyme site into the DNA fragment - (reply: 3)
724. Issues with ligation - (reply: 2)
725. Ligation of double insert into pET31 failing - (reply: 1)
726. Homemade MiniPreps for Plasmid Purification - (reply: 1)
727. Agar has dried out during cultivation - (reply: 1)
728. Gateway cloning vector compatibility - (reply: 2)
729. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
730. Cloning of IL8-promoter - (reply: 3)
731. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
732. Template DNA (plasmid) concentration for PCR - (reply: 4)
733. Ligation of 16kb PCR product - (reply: 5)
734. stable transfection - (reply: 1)
735. How to choose the right plasmids for certain types of cell? - (reply: 1)
736. Ligation needed after SDM with PfuX? - (reply: 3)
737. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
738. Cloning promoter (overlap with another gene!) - (reply: 3)
739. bacterial transfeomation - (reply: 2)
740. Loss of insert? - (reply: 1)
741. Sequencing trouble - (reply: 7)
742. Platinum-A cell trasnfection toruble - (reply: 1)
743. Cloning a promoter - (reply: 2)
744. pcDNA 3.1 His Expression - (reply: 2)
745. Cloning two fragments into a vector - (reply: 3)
746. confirmation of insert in to vector - (reply: 3)
747. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
748. Question about Molar ratio - (reply: 4)
749. Ligation problem - (reply: 16)
750. double digestion with sac1, hindIII - (reply: 3)