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Top : New Forum Archives (2009-): : Molecular-Cloning
721. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
722. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
723. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
724. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
725. competent cells stock - (reply: 3)
726. Questions about pIRES-hrGFP1a - (reply: 1)
727. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
728. Cloning into lentiviral vector - (reply: 11)
729. cloning failure help plzzz - cloning (reply: 1)
730. use of pGEM -tT easy vectors - (reply: 2)
731. ~200kb Plasmid DNA purification is not working - (reply: 1)
732. ligating a 6 His tag to plasmid - (reply: 2)
733. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
734. ligation and transformation problem - (reply: 1)
735. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
736. Mini prep of frozen bacteria cells - (reply: 2)
737. PCR cloning problem - could not get colony (reply: 4)
738. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
739. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
740. PCR clonning - (reply: 3)
741. 2 bands in blue colony PCR - (reply: 5)
742. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
743. Introduction to vector development - Basics on vectors types and construction (reply: 1)
744. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
745. Targeted mutagenesis using a linear fragment - (reply: 1)
746. Transformation & Minimal Plates - (reply: 5)
747. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
748. Subcloning Problem - (reply: 3)
749. Restriction Enzyme sites in my genes - (reply: 1)
750. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)