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Top : New Forum Archives (2009-): : Molecular-Cloning
721. Screening for clones - (reply: 2)
722. Plasmid isolation Qiagen - (reply: 2)
723. Restriction Site Destruction - (reply: 1)
724. The copy number of pPICZ alpha A - (reply: 6)
725. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
726. need help with subcloning !! - (reply: 2)
727. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
728. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
729. cdna library. - (reply: 2)
730. Help in RE digestion of vector - (reply: 5)
731. bacterial transformation problem - (reply: 2)
732. Transformation - (reply: 3)
733. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
734. What does vortexing do in transformation? - (reply: 1)
735. failure in miniprep - (reply: 1)
736. Host cells for transformation - (reply: 3)
737. making recombinant protein - (reply: 2)
738. Confirming insert???? - (reply: 1)
739. how to make a recombinant protein with signal peptide - (reply: 1)
740. How to add restriction enzyme site into the DNA fragment - (reply: 3)
741. Issues with ligation - (reply: 2)
742. Ligation of double insert into pET31 failing - (reply: 1)
743. Homemade MiniPreps for Plasmid Purification - (reply: 1)
744. Agar has dried out during cultivation - (reply: 1)
745. Gateway cloning vector compatibility - (reply: 2)
746. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
747. Cloning of IL8-promoter - (reply: 3)
748. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
749. Template DNA (plasmid) concentration for PCR - (reply: 4)
750. Ligation of 16kb PCR product - (reply: 5)