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Top : New Forum Archives (2009-): : Molecular-Cloning
721. need help with subcloning !! - (reply: 2)
722. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
723. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
724. cdna library. - (reply: 2)
725. Help in RE digestion of vector - (reply: 5)
726. bacterial transformation problem - (reply: 2)
727. Transformation - (reply: 3)
728. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
729. What does vortexing do in transformation? - (reply: 1)
730. failure in miniprep - (reply: 1)
731. Host cells for transformation - (reply: 3)
732. making recombinant protein - (reply: 2)
733. Confirming insert???? - (reply: 1)
734. how to make a recombinant protein with signal peptide - (reply: 1)
735. How to add restriction enzyme site into the DNA fragment - (reply: 3)
736. Issues with ligation - (reply: 2)
737. Ligation of double insert into pET31 failing - (reply: 1)
738. Homemade MiniPreps for Plasmid Purification - (reply: 1)
739. Agar has dried out during cultivation - (reply: 1)
740. Gateway cloning vector compatibility - (reply: 2)
741. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
742. Cloning of IL8-promoter - (reply: 3)
743. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
744. Template DNA (plasmid) concentration for PCR - (reply: 4)
745. Ligation of 16kb PCR product - (reply: 5)
746. stable transfection - (reply: 1)
747. How to choose the right plasmids for certain types of cell? - (reply: 1)
748. Ligation needed after SDM with PfuX? - (reply: 3)
749. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
750. Cloning promoter (overlap with another gene!) - (reply: 3)