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Top : New Forum Archives (2009-): : Molecular-Cloning
721. Transformation - (reply: 3)
722. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
723. What does vortexing do in transformation? - (reply: 1)
724. failure in miniprep - (reply: 1)
725. Host cells for transformation - (reply: 3)
726. making recombinant protein - (reply: 2)
727. Confirming insert???? - (reply: 1)
728. how to make a recombinant protein with signal peptide - (reply: 1)
729. How to add restriction enzyme site into the DNA fragment - (reply: 3)
730. Issues with ligation - (reply: 2)
731. Ligation of double insert into pET31 failing - (reply: 1)
732. Homemade MiniPreps for Plasmid Purification - (reply: 1)
733. Agar has dried out during cultivation - (reply: 1)
734. Gateway cloning vector compatibility - (reply: 2)
735. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
736. Cloning of IL8-promoter - (reply: 3)
737. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
738. Template DNA (plasmid) concentration for PCR - (reply: 4)
739. Ligation of 16kb PCR product - (reply: 5)
740. stable transfection - (reply: 1)
741. How to choose the right plasmids for certain types of cell? - (reply: 1)
742. Ligation needed after SDM with PfuX? - (reply: 3)
743. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
744. Cloning promoter (overlap with another gene!) - (reply: 3)
745. bacterial transfeomation - (reply: 2)
746. Loss of insert? - (reply: 1)
747. Sequencing trouble - (reply: 7)
748. Platinum-A cell trasnfection toruble - (reply: 1)
749. Cloning a promoter - (reply: 2)
750. pcDNA 3.1 His Expression - (reply: 2)