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Top : New Forum Archives (2009-): : Molecular-Cloning
721. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
722. Template DNA (plasmid) concentration for PCR - (reply: 4)
723. Ligation of 16kb PCR product - (reply: 5)
724. stable transfection - (reply: 1)
725. How to choose the right plasmids for certain types of cell? - (reply: 1)
726. Ligation needed after SDM with PfuX? - (reply: 3)
727. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
728. Cloning promoter (overlap with another gene!) - (reply: 3)
729. bacterial transfeomation - (reply: 2)
730. Loss of insert? - (reply: 1)
731. Sequencing trouble - (reply: 7)
732. Platinum-A cell trasnfection toruble - (reply: 1)
733. Cloning a promoter - (reply: 2)
734. pcDNA 3.1 His Expression - (reply: 2)
735. Cloning two fragments into a vector - (reply: 3)
736. confirmation of insert in to vector - (reply: 3)
737. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
738. Question about Molar ratio - (reply: 4)
739. Ligation problem - (reply: 16)
740. double digestion with sac1, hindIII - (reply: 3)
741. self-ligation Anneled oligos - (reply: 7)
742. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
743. Looking for a GFP plasmid (non-amp) - (reply: 3)
744. clonning problem (recombined by-products) - (reply: 8)
745. Genecloning three types of molecules? - (reply: 11)
746. help in digesting DNA fragment using restriction enzyme - (reply: 2)
747. Tansformation fails ,how can i make it ? - (reply: 3)
748. pelB leader - how to use - (reply: 1)
749. Transformation bacteria heat chock and incubation? - (reply: 2)
750. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)