Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
721. Cloning App Released from Life Technologies - (reply: 2)
722. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
723. Dissolve IPTG in EtOH - (reply: 3)
724. problem with transient transfection - (reply: 6)
725. Recombinant construct looks smaller than empty vector - (reply: 3)
726. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
727. hygromycin concentration for e-coli selection - (reply: 1)
728. orientation of promoter into viral construct - (reply: 1)
729. problems with PCR confirmation of insert . HELP ! - (reply: 1)
730. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
731. Loss of promoter during ligation - (reply: 2)
732. Enzyme restriction - (reply: 8)
733. Colony PCR Problem!!!! - (reply: 5)
734. Removing unwanted sequence from pUC19 - (reply: 4)
735. transformation fails, bacteria grows in control plate - (reply: 6)
736. competentce cell, cloning - (reply: 2)
737. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
738. co-transformation - (reply: 3)
739. pcr clean-up fail? - (reply: 1)
740. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
741. sequential cloning of multiple PCR products - how to do? (reply: 3)
742. Cloning large gene - (reply: 3)
743. Glycosilated peptide's cDNA cloning - (reply: 3)
744. pWM91 cloning problem - (reply: 4)
745. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
746. Transformation - Transformation (reply: 1)
747. how to add 3XHA to the N-terminal of a protein - (reply: 5)
748. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
749. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
750. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)