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Top : New Forum Archives (2009-): : Molecular-Cloning
721. how to make a recombinant protein with signal peptide - (reply: 1)
722. How to add restriction enzyme site into the DNA fragment - (reply: 3)
723. Issues with ligation - (reply: 2)
724. Ligation of double insert into pET31 failing - (reply: 1)
725. Homemade MiniPreps for Plasmid Purification - (reply: 1)
726. Agar has dried out during cultivation - (reply: 1)
727. Gateway cloning vector compatibility - (reply: 2)
728. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
729. Cloning of IL8-promoter - (reply: 3)
730. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
731. Template DNA (plasmid) concentration for PCR - (reply: 4)
732. Ligation of 16kb PCR product - (reply: 5)
733. stable transfection - (reply: 1)
734. How to choose the right plasmids for certain types of cell? - (reply: 1)
735. Ligation needed after SDM with PfuX? - (reply: 3)
736. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
737. Cloning promoter (overlap with another gene!) - (reply: 3)
738. bacterial transfeomation - (reply: 2)
739. Loss of insert? - (reply: 1)
740. Sequencing trouble - (reply: 7)
741. Platinum-A cell trasnfection toruble - (reply: 1)
742. Cloning a promoter - (reply: 2)
743. pcDNA 3.1 His Expression - (reply: 2)
744. Cloning two fragments into a vector - (reply: 3)
745. confirmation of insert in to vector - (reply: 3)
746. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
747. Question about Molar ratio - (reply: 4)
748. Ligation problem - (reply: 16)
749. double digestion with sac1, hindIII - (reply: 3)
750. self-ligation Anneled oligos - (reply: 7)