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Top : New Forum Archives (2009-): : Molecular-Cloning
571. Problem with ligating a small insert (LoxP site) - (reply: 5)
572. Is the genomic DNA similar among different cell lines? - (reply: 4)
573. molecular cloning - (reply: 3)
574. Swich selection in plasmid - (reply: 3)
575. Sub-cloning and transformation - (reply: 17)
576. cloning without phosphorylation - (reply: 7)
577. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
578. A good vector to make gene library? - (reply: 5)
579. problems with midiprep - (reply: 3)
580. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
581. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
582. 900bp insertion into PIRESnEO,problem - (reply: 2)
583. fusion of cDNA expressing protein with gfp - (reply: 1)
584. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
585. Problem with plasmids transformation - (reply: 6)
586. Cloning genetic sequences of proteins. - (reply: 1)
587. problem of restriction enzyme digestion - (reply: 3)
588. Site-directed, PCR works but no colonies - (reply: 2)
589. Simoltanious Multi-restriction digestion - (reply: 3)
590. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
591. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
592. Restriction digestion problems - (reply: 3)
593. should I do blunt end my pcr product before ligation - (reply: 2)
594. Maxiprep of freeze/thawed pellet. - (reply: 1)
595. Digest with AarI and Pml I - (reply: 1)
596. Help with CGL gene cloning problems - (reply: 1)
597. screening for GPF - (reply: 10)
598. Creating a pGEM-T easy + insert sequence map - (reply: 1)
599. where to get vector overexpressing caveolin-1? - (reply: 2)
600. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)