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Top : New Forum Archives (2009-): : Molecular-Cloning
571. DH10B with big construct question - (reply: 2)
572. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
573. cloning to pET28a - (reply: 1)
574. Weird Library Transformation Results - (reply: 10)
575. Injecting mice with lentivirus - (reply: 1)
576. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
577. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
578. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
579. Ligation into a BAC? - (reply: 2)
580. oligo continually appears less on gel compared to other same load - (reply: 2)
581. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
582. plasmid tetR and tet promoter - (reply: 2)
583. unable to amplify an infectious retrovirus clone - (reply: 4)
584. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
585. No bands at all after double digestion - (reply: 1)
586. Digest for insert orientation - (reply: 4)
587. Colonies from transformation plate are not growing...:( - (reply: 5)
588. Problem with ligating a small insert (LoxP site) - (reply: 5)
589. Is the genomic DNA similar among different cell lines? - (reply: 4)
590. molecular cloning - (reply: 3)
591. Swich selection in plasmid - (reply: 3)
592. Sub-cloning and transformation - (reply: 17)
593. cloning without phosphorylation - (reply: 7)
594. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
595. A good vector to make gene library? - (reply: 5)
596. problems with midiprep - (reply: 3)
597. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
598. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
599. 900bp insertion into PIRESnEO,problem - (reply: 2)
600. fusion of cDNA expressing protein with gfp - (reply: 1)