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Top : New Forum Archives (2009-): : Molecular-Cloning
571. Colony PCR and digestion with KpnI - (reply: 10)
572. Greenish tinge to bacterial culture - (reply: 3)
573. SURE E. coli competent cells - (reply: 1)
574. Ligation - (reply: 20)
575. E. coli growing on plates and bot not in LB medium - (reply: 10)
576. *** Human Insulin promoter sequence*** - (reply: 2)
577. digested plasmid - (reply: 2)
578. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
579. Subcloning - RE digest and ligation and transformation - (reply: 5)
580. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
581. Making DNA Linkers for cloning purposes - (reply: 1)
582. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
583. Dam-/+ e. coli strains - (reply: 6)
584. cloning short genomic DNA fragments - (reply: 1)
585. finding the protein and nucleotide matches - (reply: 1)
586. DH10B with big construct question - (reply: 2)
587. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
588. cloning to pET28a - (reply: 1)
589. Weird Library Transformation Results - (reply: 10)
590. Injecting mice with lentivirus - (reply: 1)
591. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
592. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
593. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
594. Ligation into a BAC? - (reply: 2)
595. oligo continually appears less on gel compared to other same load - (reply: 2)
596. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
597. plasmid tetR and tet promoter - (reply: 2)
598. unable to amplify an infectious retrovirus clone - (reply: 4)
599. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
600. No bands at all after double digestion - (reply: 1)