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Top : New Forum Archives (2009-): : Molecular-Cloning
571. cloning short genomic DNA fragments - (reply: 1)
572. finding the protein and nucleotide matches - (reply: 1)
573. DH10B with big construct question - (reply: 2)
574. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
575. cloning to pET28a - (reply: 1)
576. Weird Library Transformation Results - (reply: 10)
577. Injecting mice with lentivirus - (reply: 1)
578. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
579. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
580. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
581. Ligation into a BAC? - (reply: 2)
582. oligo continually appears less on gel compared to other same load - (reply: 2)
583. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
584. plasmid tetR and tet promoter - (reply: 2)
585. unable to amplify an infectious retrovirus clone - (reply: 4)
586. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
587. No bands at all after double digestion - (reply: 1)
588. Digest for insert orientation - (reply: 4)
589. Colonies from transformation plate are not growing...:( - (reply: 5)
590. Problem with ligating a small insert (LoxP site) - (reply: 5)
591. Is the genomic DNA similar among different cell lines? - (reply: 4)
592. molecular cloning - (reply: 3)
593. Swich selection in plasmid - (reply: 3)
594. Sub-cloning and transformation - (reply: 17)
595. cloning without phosphorylation - (reply: 7)
596. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
597. A good vector to make gene library? - (reply: 5)
598. problems with midiprep - (reply: 3)
599. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
600. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)