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Top : New Forum Archives (2009-): : Molecular-Cloning
571. Ligation - (reply: 20)
572. E. coli growing on plates and bot not in LB medium - (reply: 10)
573. *** Human Insulin promoter sequence*** - (reply: 2)
574. digested plasmid - (reply: 2)
575. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
576. Subcloning - RE digest and ligation and transformation - (reply: 5)
577. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
578. Making DNA Linkers for cloning purposes - (reply: 1)
579. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
580. Dam-/+ e. coli strains - (reply: 6)
581. cloning short genomic DNA fragments - (reply: 1)
582. finding the protein and nucleotide matches - (reply: 1)
583. DH10B with big construct question - (reply: 2)
584. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
585. cloning to pET28a - (reply: 1)
586. Weird Library Transformation Results - (reply: 10)
587. Injecting mice with lentivirus - (reply: 1)
588. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
589. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
590. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
591. Ligation into a BAC? - (reply: 2)
592. oligo continually appears less on gel compared to other same load - (reply: 2)
593. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
594. plasmid tetR and tet promoter - (reply: 2)
595. unable to amplify an infectious retrovirus clone - (reply: 4)
596. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
597. No bands at all after double digestion - (reply: 1)
598. Digest for insert orientation - (reply: 4)
599. Colonies from transformation plate are not growing...:( - (reply: 5)
600. Problem with ligating a small insert (LoxP site) - (reply: 5)