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Top : New Forum Archives (2009-): : Molecular-Cloning
571. digested plasmid - (reply: 2)
572. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
573. Subcloning - RE digest and ligation and transformation - (reply: 5)
574. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
575. Making DNA Linkers for cloning purposes - (reply: 1)
576. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
577. Dam-/+ e. coli strains - (reply: 6)
578. cloning short genomic DNA fragments - (reply: 1)
579. finding the protein and nucleotide matches - (reply: 1)
580. DH10B with big construct question - (reply: 2)
581. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
582. cloning to pET28a - (reply: 1)
583. Weird Library Transformation Results - (reply: 10)
584. Injecting mice with lentivirus - (reply: 1)
585. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
586. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
587. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
588. Ligation into a BAC? - (reply: 2)
589. oligo continually appears less on gel compared to other same load - (reply: 2)
590. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
591. plasmid tetR and tet promoter - (reply: 2)
592. unable to amplify an infectious retrovirus clone - (reply: 4)
593. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
594. No bands at all after double digestion - (reply: 1)
595. Digest for insert orientation - (reply: 4)
596. Colonies from transformation plate are not growing...:( - (reply: 5)
597. Problem with ligating a small insert (LoxP site) - (reply: 5)
598. Is the genomic DNA similar among different cell lines? - (reply: 4)
599. molecular cloning - (reply: 3)
600. Swich selection in plasmid - (reply: 3)