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Top : New Forum Archives (2009-): : Molecular-Cloning
571. problems with recovery of dna from gel and ligation - (reply: 5)
572. Colony PCR and digestion with KpnI - (reply: 10)
573. Greenish tinge to bacterial culture - (reply: 3)
574. SURE E. coli competent cells - (reply: 1)
575. Ligation - (reply: 20)
576. E. coli growing on plates and bot not in LB medium - (reply: 10)
577. *** Human Insulin promoter sequence*** - (reply: 2)
578. digested plasmid - (reply: 2)
579. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
580. Subcloning - RE digest and ligation and transformation - (reply: 5)
581. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
582. Making DNA Linkers for cloning purposes - (reply: 1)
583. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
584. Dam-/+ e. coli strains - (reply: 6)
585. cloning short genomic DNA fragments - (reply: 1)
586. finding the protein and nucleotide matches - (reply: 1)
587. DH10B with big construct question - (reply: 2)
588. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
589. cloning to pET28a - (reply: 1)
590. Weird Library Transformation Results - (reply: 10)
591. Injecting mice with lentivirus - (reply: 1)
592. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
593. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
594. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
595. Ligation into a BAC? - (reply: 2)
596. oligo continually appears less on gel compared to other same load - (reply: 2)
597. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
598. plasmid tetR and tet promoter - (reply: 2)
599. unable to amplify an infectious retrovirus clone - (reply: 4)
600. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)