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Top : New Forum Archives (2009-): : Molecular-Cloning
571. Transformation with a big plasmid - (reply: 4)
572. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
573. Three way ligation problem - (reply: 6)
574. problems with recovery of dna from gel and ligation - (reply: 5)
575. Colony PCR and digestion with KpnI - (reply: 10)
576. Greenish tinge to bacterial culture - (reply: 3)
577. SURE E. coli competent cells - (reply: 1)
578. Ligation - (reply: 20)
579. E. coli growing on plates and bot not in LB medium - (reply: 10)
580. *** Human Insulin promoter sequence*** - (reply: 2)
581. digested plasmid - (reply: 2)
582. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
583. Subcloning - RE digest and ligation and transformation - (reply: 5)
584. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
585. Making DNA Linkers for cloning purposes - (reply: 1)
586. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
587. Dam-/+ e. coli strains - (reply: 6)
588. cloning short genomic DNA fragments - (reply: 1)
589. finding the protein and nucleotide matches - (reply: 1)
590. DH10B with big construct question - (reply: 2)
591. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
592. cloning to pET28a - (reply: 1)
593. Weird Library Transformation Results - (reply: 10)
594. Injecting mice with lentivirus - (reply: 1)
595. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
596. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
597. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
598. Ligation into a BAC? - (reply: 2)
599. oligo continually appears less on gel compared to other same load - (reply: 2)
600. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)