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Top : New Forum Archives (2009-): : Molecular-Cloning
571. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
572. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
573. Restriction digestion problems - (reply: 3)
574. should I do blunt end my pcr product before ligation - (reply: 2)
575. Maxiprep of freeze/thawed pellet. - (reply: 1)
576. Digest with AarI and Pml I - (reply: 1)
577. Help with CGL gene cloning problems - (reply: 1)
578. screening for GPF - (reply: 10)
579. Creating a pGEM-T easy + insert sequence map - (reply: 1)
580. where to get vector overexpressing caveolin-1? - (reply: 2)
581. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
582. pINDUCER series - (reply: 1)
583. LZRS vector details - (reply: 1)
584. 12.4kb vector 6.7kb insert - (reply: 2)
585. Stable transfection - (reply: 2)
586. Using normal competent cells in electroporation method? - (reply: 1)
587. an effective way to do a yeast colony pcr - (reply: 2)
588. pGEM-T Easy vector contamination - (reply: 2)
589. recover old transformed DH5 - (reply: 1)
590. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
591. oligo(dt) 15 vs random primers - (reply: 3)
592. I need bynary vector pART27 - (reply: 1)
593. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
594. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
595. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
596. cloning into Pgem t easy - (reply: 1)
597. How to get pure protein of a gene - (reply: 3)
598. How to insert genes into chromosome of E. coli - (reply: 1)
599. Gene synthesis - (reply: 2)
600. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)