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Top : New Forum Archives (2009-): : Molecular-Cloning
571. I need bynary vector pART27 - (reply: 1)
572. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
573. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
574. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
575. cloning into Pgem t easy - (reply: 1)
576. How to get pure protein of a gene - (reply: 3)
577. How to insert genes into chromosome of E. coli - (reply: 1)
578. Gene synthesis - (reply: 2)
579. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
580. Pls help me with this plasmid!!! - (reply: 1)
581. Problems with Restriction Digest Results - (reply: 7)
582. Methods of plasmid transformation? only heat shock? - (reply: 1)
583. Digestion - (reply: 2)
584. Digestion of Genomic DNA - (reply: 1)
585. Isolation of a Gene of Interest for Subcloning - (reply: 8)
586. E.coli grow on neg. control - (reply: 1)
587. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
588. Need help troubleshooting digest. - (reply: 1)
589. Competent cells question: JM101 vs DH5alpha - (reply: 2)
590. How to avoid plasmid instability - (reply: 4)
591. Question Help - (reply: 1)
592. Shear-induced damage of plasmid by vortexing - (reply: 1)
593. Problems with blunt end ligation - (reply: 3)
594. Problem with Transforming E. coli DH10Bac - (reply: 8)
595. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
596. Heat inactivate ligase - (reply: 5)
597. DMSO stock of overnight bacterial cultures - (reply: 1)
598. Recommended competent cell for transformation of large plasmids? - (reply: 4)
599. Prolem in screening of hygromycin concentration - (reply: 1)
600. Primers for Introduction of new restriction sites to a vector - (reply: 3)