Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
571. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
572. cloning to pET28a - (reply: 1)
573. Weird Library Transformation Results - (reply: 10)
574. Injecting mice with lentivirus - (reply: 1)
575. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
576. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
577. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
578. Ligation into a BAC? - (reply: 2)
579. oligo continually appears less on gel compared to other same load - (reply: 2)
580. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
581. plasmid tetR and tet promoter - (reply: 2)
582. unable to amplify an infectious retrovirus clone - (reply: 4)
583. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
584. No bands at all after double digestion - (reply: 1)
585. Digest for insert orientation - (reply: 4)
586. Colonies from transformation plate are not growing...:( - (reply: 5)
587. Problem with ligating a small insert (LoxP site) - (reply: 5)
588. Is the genomic DNA similar among different cell lines? - (reply: 4)
589. molecular cloning - (reply: 3)
590. Swich selection in plasmid - (reply: 3)
591. Sub-cloning and transformation - (reply: 17)
592. cloning without phosphorylation - (reply: 7)
593. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
594. A good vector to make gene library? - (reply: 5)
595. problems with midiprep - (reply: 3)
596. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
597. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
598. 900bp insertion into PIRESnEO,problem - (reply: 2)
599. fusion of cDNA expressing protein with gfp - (reply: 1)
600. Did my EcoRI restriction digestion work? picture included! - (reply: 49)