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Top : New Forum Archives (2009-): : Molecular-Cloning
571. Problems with Restriction Digest Results - (reply: 7)
572. Methods of plasmid transformation? only heat shock? - (reply: 1)
573. Digestion - (reply: 2)
574. Digestion of Genomic DNA - (reply: 1)
575. Isolation of a Gene of Interest for Subcloning - (reply: 8)
576. E.coli grow on neg. control - (reply: 1)
577. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
578. Need help troubleshooting digest. - (reply: 1)
579. Competent cells question: JM101 vs DH5alpha - (reply: 2)
580. How to avoid plasmid instability - (reply: 4)
581. Question Help - (reply: 1)
582. Shear-induced damage of plasmid by vortexing - (reply: 1)
583. Problems with blunt end ligation - (reply: 3)
584. Problem with Transforming E. coli DH10Bac - (reply: 8)
585. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
586. Heat inactivate ligase - (reply: 5)
587. DMSO stock of overnight bacterial cultures - (reply: 1)
588. Recommended competent cell for transformation of large plasmids? - (reply: 4)
589. Prolem in screening of hygromycin concentration - (reply: 1)
590. Primers for Introduction of new restriction sites to a vector - (reply: 3)
591. problem in cloning involving partial digestion - (reply: 1)
592. Why perform restriction enzyme digestion again? - (reply: 1)
593. Cotransient Transfections DNA Requirements - (reply: 3)
594. which software to draw the scheme of cloning strategy - (reply: 5)
595. Help with restriction analysis - (reply: 1)
596. Sequencing of PCR product - (reply: 4)
597. KanR sequence - (reply: 2)
598. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
599. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
600. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)