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Top : New Forum Archives (2009-): : Molecular-Cloning
571. cloning without phosphorylation - (reply: 7)
572. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
573. A good vector to make gene library? - (reply: 5)
574. problems with midiprep - (reply: 3)
575. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
576. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
577. 900bp insertion into PIRESnEO,problem - (reply: 2)
578. fusion of cDNA expressing protein with gfp - (reply: 1)
579. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
580. Problem with plasmids transformation - (reply: 6)
581. Cloning genetic sequences of proteins. - (reply: 1)
582. problem of restriction enzyme digestion - (reply: 3)
583. Site-directed, PCR works but no colonies - (reply: 2)
584. Simoltanious Multi-restriction digestion - (reply: 3)
585. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
586. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
587. Restriction digestion problems - (reply: 3)
588. should I do blunt end my pcr product before ligation - (reply: 2)
589. Maxiprep of freeze/thawed pellet. - (reply: 1)
590. Digest with AarI and Pml I - (reply: 1)
591. Help with CGL gene cloning problems - (reply: 1)
592. screening for GPF - (reply: 10)
593. Creating a pGEM-T easy + insert sequence map - (reply: 1)
594. where to get vector overexpressing caveolin-1? - (reply: 2)
595. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
596. pINDUCER series - (reply: 1)
597. LZRS vector details - (reply: 1)
598. 12.4kb vector 6.7kb insert - (reply: 2)
599. Stable transfection - (reply: 2)
600. Using normal competent cells in electroporation method? - (reply: 1)