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Top : New Forum Archives (2009-): : Molecular-Cloning
571. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
572. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
573. Plasmid digestion after transformation - (reply: 4)
574. Blunt end ligation insert:vector ratio - (reply: 1)
575. PCRed on restriction sites -- how do I purify it? - (reply: 8)
576. Puzzle about plamid and its digest, plz help - (reply: 8)
577. Smear above plasmid dna - (reply: 3)
578. restriction digestion buffers contaminated with dnase - (reply: 3)
579. Bacterial Induction - (reply: 1)
580. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
581. smear restriction digest - (reply: 1)
582. How to set the Sonicator to 20%amplitude? - (reply: 3)
583. No DNA after midiprep - (reply: 2)
584. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
585. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
586. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
587. Impact of primer sequence on TA cloning - (reply: 3)
588. Maximum length of cDNA from RT reaction - (reply: 6)
589. overlapping PCR protocol - (reply: 12)
590. cloning an AT rich gene - (reply: 3)
591. Cloning two genes in the same plasmid - (reply: 4)
592. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
593. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
594. Problem: Cloning DNA shuffled band - (reply: 2)
595. shRNA to primary keratinocytes - (reply: 1)
596. Enzymetic digestion with ApaI and HincII - (reply: 2)
597. Ligation gel picture interpretation - (reply: 4)
598. strange subcloning problem - (reply: 9)
599. Cloned sequence is not predicted size by western blot - (reply: 1)
600. Ligation of insert to itself problem - (reply: 3)