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Top : New Forum Archives (2009-): : Molecular-Cloning
571. Ligation into a BAC? - (reply: 2)
572. oligo continually appears less on gel compared to other same load - (reply: 2)
573. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
574. plasmid tetR and tet promoter - (reply: 2)
575. unable to amplify an infectious retrovirus clone - (reply: 4)
576. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
577. No bands at all after double digestion - (reply: 1)
578. Digest for insert orientation - (reply: 4)
579. Colonies from transformation plate are not growing...:( - (reply: 5)
580. Problem with ligating a small insert (LoxP site) - (reply: 5)
581. Is the genomic DNA similar among different cell lines? - (reply: 4)
582. molecular cloning - (reply: 3)
583. Swich selection in plasmid - (reply: 3)
584. Sub-cloning and transformation - (reply: 17)
585. cloning without phosphorylation - (reply: 7)
586. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
587. A good vector to make gene library? - (reply: 5)
588. problems with midiprep - (reply: 3)
589. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
590. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
591. 900bp insertion into PIRESnEO,problem - (reply: 2)
592. fusion of cDNA expressing protein with gfp - (reply: 1)
593. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
594. Problem with plasmids transformation - (reply: 6)
595. Cloning genetic sequences of proteins. - (reply: 1)
596. problem of restriction enzyme digestion - (reply: 3)
597. Site-directed, PCR works but no colonies - (reply: 2)
598. Simoltanious Multi-restriction digestion - (reply: 3)
599. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
600. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)