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Top : New Forum Archives (2009-): : Molecular-Cloning
571. oligo continually appears less on gel compared to other same load - (reply: 2)
572. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
573. plasmid tetR and tet promoter - (reply: 2)
574. unable to amplify an infectious retrovirus clone - (reply: 4)
575. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
576. No bands at all after double digestion - (reply: 1)
577. Digest for insert orientation - (reply: 4)
578. Colonies from transformation plate are not growing...:( - (reply: 5)
579. Problem with ligating a small insert (LoxP site) - (reply: 5)
580. Is the genomic DNA similar among different cell lines? - (reply: 4)
581. molecular cloning - (reply: 3)
582. Swich selection in plasmid - (reply: 3)
583. Sub-cloning and transformation - (reply: 17)
584. cloning without phosphorylation - (reply: 7)
585. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
586. A good vector to make gene library? - (reply: 5)
587. problems with midiprep - (reply: 3)
588. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
589. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
590. 900bp insertion into PIRESnEO,problem - (reply: 2)
591. fusion of cDNA expressing protein with gfp - (reply: 1)
592. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
593. Problem with plasmids transformation - (reply: 6)
594. Cloning genetic sequences of proteins. - (reply: 1)
595. problem of restriction enzyme digestion - (reply: 3)
596. Site-directed, PCR works but no colonies - (reply: 2)
597. Simoltanious Multi-restriction digestion - (reply: 3)
598. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
599. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
600. Restriction digestion problems - (reply: 3)