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Top : New Forum Archives (2009-): : Molecular-Cloning
571. How to avoid plasmid instability - (reply: 4)
572. Question Help - (reply: 1)
573. Shear-induced damage of plasmid by vortexing - (reply: 1)
574. Problems with blunt end ligation - (reply: 3)
575. Problem with Transforming E. coli DH10Bac - (reply: 8)
576. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
577. Heat inactivate ligase - (reply: 5)
578. DMSO stock of overnight bacterial cultures - (reply: 1)
579. Recommended competent cell for transformation of large plasmids? - (reply: 4)
580. Prolem in screening of hygromycin concentration - (reply: 1)
581. Primers for Introduction of new restriction sites to a vector - (reply: 3)
582. problem in cloning involving partial digestion - (reply: 1)
583. Why perform restriction enzyme digestion again? - (reply: 1)
584. Cotransient Transfections DNA Requirements - (reply: 3)
585. which software to draw the scheme of cloning strategy - (reply: 5)
586. Help with restriction analysis - (reply: 1)
587. Sequencing of PCR product - (reply: 4)
588. KanR sequence - (reply: 2)
589. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
590. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
591. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
592. Plasmid digestion after transformation - (reply: 4)
593. Blunt end ligation insert:vector ratio - (reply: 1)
594. PCRed on restriction sites -- how do I purify it? - (reply: 8)
595. Puzzle about plamid and its digest, plz help - (reply: 8)
596. Smear above plasmid dna - (reply: 3)
597. restriction digestion buffers contaminated with dnase - (reply: 3)
598. Bacterial Induction - (reply: 1)
599. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
600. smear restriction digest - (reply: 1)