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Top : New Forum Archives (2009-): : Molecular-Cloning
571. No DNA after midiprep - (reply: 2)
572. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
573. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
574. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
575. Impact of primer sequence on TA cloning - (reply: 3)
576. Maximum length of cDNA from RT reaction - (reply: 6)
577. overlapping PCR protocol - (reply: 12)
578. cloning an AT rich gene - (reply: 3)
579. Cloning two genes in the same plasmid - (reply: 4)
580. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
581. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
582. Problem: Cloning DNA shuffled band - (reply: 2)
583. shRNA to primary keratinocytes - (reply: 1)
584. Enzymetic digestion with ApaI and HincII - (reply: 2)
585. Ligation gel picture interpretation - (reply: 4)
586. strange subcloning problem - (reply: 9)
587. Cloned sequence is not predicted size by western blot - (reply: 1)
588. Ligation of insert to itself problem - (reply: 3)
589. double digest problem - (reply: 3)
590. Difficulty in expressing fusion protein - (reply: 1)
591. Plasmid recovery problem - (reply: 3)
592. Restriction Digest Problem - (reply: 5)
593. Recombination protocol needed - (reply: 2)
594. Cloning problem using pET32a vector - (reply: 3)
595. Fixing SNPs after cloning - (reply: 5)
596. Electroporated plasmid loses the insert - (reply: 8)
597. Recombination due to overselective plate? - (reply: 11)
598. Help with ligation - (reply: 2)
599. emty vector cloning - (reply: 3)
600. RE site on complimentary strand of plasmid? - (reply: 1)