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Top : New Forum Archives (2009-): : Molecular-Cloning
571. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
572. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
573. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
574. Ligation into a BAC? - (reply: 2)
575. oligo continually appears less on gel compared to other same load - (reply: 2)
576. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
577. plasmid tetR and tet promoter - (reply: 2)
578. unable to amplify an infectious retrovirus clone - (reply: 4)
579. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
580. No bands at all after double digestion - (reply: 1)
581. Digest for insert orientation - (reply: 4)
582. Colonies from transformation plate are not growing...:( - (reply: 5)
583. Problem with ligating a small insert (LoxP site) - (reply: 5)
584. Is the genomic DNA similar among different cell lines? - (reply: 4)
585. molecular cloning - (reply: 3)
586. Swich selection in plasmid - (reply: 3)
587. Sub-cloning and transformation - (reply: 17)
588. cloning without phosphorylation - (reply: 7)
589. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
590. A good vector to make gene library? - (reply: 5)
591. problems with midiprep - (reply: 3)
592. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
593. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
594. 900bp insertion into PIRESnEO,problem - (reply: 2)
595. fusion of cDNA expressing protein with gfp - (reply: 1)
596. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
597. Problem with plasmids transformation - (reply: 6)
598. Cloning genetic sequences of proteins. - (reply: 1)
599. problem of restriction enzyme digestion - (reply: 3)
600. Site-directed, PCR works but no colonies - (reply: 2)