Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
61. cloning problems using in-fusion kit - (reply: 5)
62. Mutation in one chain of homodimer - (reply: 4)
63. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
64. Alternative competent cells for QuikChange kit - (reply: 2)
65. Ligation Mistake - (reply: 4)
66. Cloning - Ligation problem - (reply: 3)
67. How to clone a 2A peptide between two ORFs? - (reply: 2)
68. preparing a vector for transfection - (reply: 2)
69. Ligation of two PCR products - (reply: 1)
70. TA Cloning - (reply: 11)
71. Troubleshootting lentiviral transduction. - (reply: 3)
72. Trouble with PCR using ligation mix as template? - (reply: 3)
73. Suitable ratio of vector and insert in cloning - (reply: 5)
74. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
75. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
76. plasmid repositories - (reply: 2)
77. How to insert PreScission protease into a vector - (reply: 1)
78. Why the insert become shorter after inserting into plasmid? - (reply: 2)
79. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
80. Thermo scientific T4 DNA ligase product help - (reply: 3)
81. Cloning a big insert in a small vector - (reply: 2)
82. Transformation E.Coli help - (reply: 2)
83. Exonucleases that won't harm circular dsDNA - (reply: 1)
84. How to produce transgenic mice? - (reply: 10)
85. No colonies on plate after liagtion - (reply: 7)
86. Question: Can I cut out rb glob PA Terminator - (reply: 1)
87. Low Yield Miniprep - (reply: 5)
88. question about Clonetech Retro-X Tet-on system - (reply: 3)
89. Clonetech Retro-X Tet-on system - (reply: 1)
90. quiaquick gel extraction troubleshooting - (reply: 1)