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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
62. Alternative competent cells for QuikChange kit - (reply: 2)
63. Ligation Mistake - (reply: 4)
64. Cloning - Ligation problem - (reply: 3)
65. How to clone a 2A peptide between two ORFs? - (reply: 2)
66. preparing a vector for transfection - (reply: 2)
67. Ligation of two PCR products - (reply: 1)
68. TA Cloning - (reply: 11)
69. Troubleshootting lentiviral transduction. - (reply: 3)
70. Trouble with PCR using ligation mix as template? - (reply: 3)
71. Suitable ratio of vector and insert in cloning - (reply: 5)
72. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
73. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
74. plasmid repositories - (reply: 2)
75. How to insert PreScission protease into a vector - (reply: 1)
76. Why the insert become shorter after inserting into plasmid? - (reply: 2)
77. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
78. Thermo scientific T4 DNA ligase product help - (reply: 3)
79. Cloning a big insert in a small vector - (reply: 2)
80. Transformation E.Coli help - (reply: 2)
81. Exonucleases that won't harm circular dsDNA - (reply: 1)
82. How to produce transgenic mice? - (reply: 10)
83. No colonies on plate after liagtion - (reply: 7)
84. Question: Can I cut out rb glob PA Terminator - (reply: 1)
85. Low Yield Miniprep - (reply: 5)
86. question about Clonetech Retro-X Tet-on system - (reply: 3)
87. Clonetech Retro-X Tet-on system - (reply: 1)
88. quiaquick gel extraction troubleshooting - (reply: 1)
89. Insert direction in TA cloning - (reply: 2)
90. Transformation in Staphylococcus epidermidis - (reply: 1)