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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Whatman FTA cards - (reply: 1)
62. Trouble with TOPO-Cloning - (reply: 3)
63. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
64. Comparing DNA fragments using restriction map - (reply: 1)
65. Doing a double digest - (reply: 3)
66. Types of igation controls - (reply: 6)
67. Problem with double digest cloning - (reply: 3)
68. Weird results with bacterial transformation - (reply: 11)
69. Site Directed Mutagenesis - no product - (reply: 4)
70. Problems sequencing ligation product - (reply: 5)
71. Faster method to screeen for positive colonies. - (reply: 8)
72. 260/230 ratio, does it matter? - (reply: 4)
73. Double digestion problem with PUCsp vector - (reply: 1)
74. How much plasmid for transformation? - (reply: 8)
75. RE digestion and ligation troubleshooting. - (reply: 1)
76. replicating a mammalian expression vector in bacteria - (reply: 3)
77. Cloning of an unknown gene - (reply: 4)
78. cloning problems using in-fusion kit - (reply: 5)
79. Mutation in one chain of homodimer - (reply: 4)
80. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
81. Alternative competent cells for QuikChange kit - (reply: 2)
82. Ligation Mistake - (reply: 4)
83. Cloning - Ligation problem - (reply: 3)
84. How to clone a 2A peptide between two ORFs? - (reply: 2)
85. preparing a vector for transfection - (reply: 2)
86. Ligation of two PCR products - (reply: 1)
87. TA Cloning - (reply: 11)
88. Troubleshootting lentiviral transduction. - (reply: 3)
89. Trouble with PCR using ligation mix as template? - (reply: 3)
90. Suitable ratio of vector and insert in cloning - (reply: 5)