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61. Ligation of large ammount of insert (no transformation) - (reply: 7)
62. PCR and restriction enzyme digestion - (reply: 3)
63. Probelm with double digestion - (reply: 1)
64. Cloning not working, all steps checked, clueless - (reply: 1)
65. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
66. recombinant protein form - (reply: 2)
67. restricted PCR plasmid runs slower - (reply: 2)
68. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
69. High frequency of vector religation - (reply: 10)
70. Cloned but not expressing! - (reply: 8)
71. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
72. DNA ligation and trasformation - (reply: 6)
73. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
74. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
75. How to set up simultaneous digestion? - (reply: 3)
76. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
77. Problem with ligation using pJET and pGEM for clone library - (reply: 9)
78. Freeze transformation mixture? - (reply: 2)
79. Cutting my plasmid made it larger - (reply: 7)
80. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
81. Amplification of CAG promoter problems - (reply: 3)
82. restriction enzyme - (reply: 2)
83. Recreate original plasmid by cutting out insert - (reply: 2)
84. Colony-PCR workaround - (reply: 3)
85. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
86. Can I pause a bacterial culture at 4°C - (reply: 2)
87. TOPO TA CLONING - LIGATION STEP - (reply: 6)
88. posting sequences of plasmid? - (reply: 3)
89. Restriction Enzyme Digest not working - (reply: 2)
90. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
62. PCR and restriction enzyme digestion - (reply: 3)
63. Probelm with double digestion - (reply: 1)
64. Cloning not working, all steps checked, clueless - (reply: 1)
65. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
66. recombinant protein form - (reply: 2)
67. restricted PCR plasmid runs slower - (reply: 2)
68. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
69. High frequency of vector religation - (reply: 10)
70. Cloned but not expressing! - (reply: 8)
71. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
72. DNA ligation and trasformation - (reply: 6)
73. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
74. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
75. How to set up simultaneous digestion? - (reply: 3)
76. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
77. Problem with ligation using pJET and pGEM for clone library - (reply: 9)
78. Freeze transformation mixture? - (reply: 2)
79. Cutting my plasmid made it larger - (reply: 7)
80. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
81. Amplification of CAG promoter problems - (reply: 3)
82. restriction enzyme - (reply: 2)
83. Recreate original plasmid by cutting out insert - (reply: 2)
84. Colony-PCR workaround - (reply: 3)
85. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
86. Can I pause a bacterial culture at 4°C - (reply: 2)
87. TOPO TA CLONING - LIGATION STEP - (reply: 6)
88. posting sequences of plasmid? - (reply: 3)
89. Restriction Enzyme Digest not working - (reply: 2)
90. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)