Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
61. TA Cloning - (reply: 11)
62. Troubleshootting lentiviral transduction. - (reply: 3)
63. Trouble with PCR using ligation mix as template? - (reply: 3)
64. Suitable ratio of vector and insert in cloning - (reply: 5)
65. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
66. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
67. plasmid repositories - (reply: 2)
68. How to insert PreScission protease into a vector - (reply: 1)
69. Why the insert become shorter after inserting into plasmid? - (reply: 2)
70. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
71. Thermo scientific T4 DNA ligase product help - (reply: 3)
72. Cloning a big insert in a small vector - (reply: 2)
73. Transformation E.Coli help - (reply: 2)
74. Exonucleases that won't harm circular dsDNA - (reply: 1)
75. How to produce transgenic mice? - (reply: 10)
76. No colonies on plate after liagtion - (reply: 7)
77. Question: Can I cut out rb glob PA Terminator - (reply: 1)
78. Low Yield Miniprep - (reply: 5)
79. question about Clonetech Retro-X Tet-on system - (reply: 3)
80. Clonetech Retro-X Tet-on system - (reply: 1)
81. quiaquick gel extraction troubleshooting - (reply: 1)
82. Insert direction in TA cloning - (reply: 2)
83. Transformation in Staphylococcus epidermidis - (reply: 1)
84. Plasmids recombining - (reply: 3)
85. troubleshooting for a simple SDM - (reply: 2)
86. Should I proceed with clone selection? - (reply: 2)
87. Need help in codon optimization... - (reply: 1)
88. transformation - (reply: 2)
89. problem in sequencing of gene - (reply: 3)
90. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)