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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Problems sequencing ligation product - (reply: 5)
62. Faster method to screeen for positive colonies. - (reply: 8)
63. 260/230 ratio, does it matter? - (reply: 4)
64. Double digestion problem with PUCsp vector - (reply: 1)
65. How much plasmid for transformation? - (reply: 8)
66. RE digestion and ligation troubleshooting. - (reply: 1)
67. replicating a mammalian expression vector in bacteria - (reply: 3)
68. Cloning of an unknown gene - (reply: 3)
69. cloning problems using in-fusion kit - (reply: 5)
70. Mutation in one chain of homodimer - (reply: 4)
71. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
72. Alternative competent cells for QuikChange kit - (reply: 2)
73. Ligation Mistake - (reply: 4)
74. Cloning - Ligation problem - (reply: 3)
75. How to clone a 2A peptide between two ORFs? - (reply: 2)
76. preparing a vector for transfection - (reply: 2)
77. Ligation of two PCR products - (reply: 1)
78. TA Cloning - (reply: 11)
79. Troubleshootting lentiviral transduction. - (reply: 3)
80. Trouble with PCR using ligation mix as template? - (reply: 3)
81. Suitable ratio of vector and insert in cloning - (reply: 5)
82. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
83. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
84. plasmid repositories - (reply: 2)
85. How to insert PreScission protease into a vector - (reply: 1)
86. Why the insert become shorter after inserting into plasmid? - (reply: 2)
87. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
88. Thermo scientific T4 DNA ligase product help - (reply: 3)
89. Cloning a big insert in a small vector - (reply: 2)
90. Transformation E.Coli help - (reply: 2)