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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Vector-insert ratio - (reply: 6)
62. Cloning contamination - (reply: 3)
63. plasmid construction - (reply: 2)
64. primer design with restriction enzyme - (reply: 1)
65. vector propagation in E coli - (reply: 1)
66. Restriction to remove background - (reply: 4)
67. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
68. Restriction and ligation time interval - (reply: 1)
69. Problem shrna cloning, bad sequencing results - (reply: 1)
70. Lentivirus copy number - (reply: 7)
71. Genomic -Lactamase? Blue White screening not working - (reply: 1)
72. direction of the expression units in multi-gene vectors - (reply: 1)
73. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
74. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
75. Measuring pcr fragments in a gel - (reply: 1)
76. gel electrophoresis - (reply: 4)
77. three questions about CRISPR knowout - (reply: 2)
78. Help needed for Ligation/Transformation - (reply: 1)
79. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
80. ClaI restriction enzyme efficency - (reply: 1)
81. Blunt end cloning - (reply: 12)
82. sequantial digest - (reply: 25)
83. Temperature sensitivity of Plasmids - (reply: 1)
84. DMSO in restriction digest - (reply: 1)
85. Molecular cloning - (reply: 5)
86. Nuclease free water for cloning - (reply: 8)
87. Directional cloning 2bps overhang - (reply: 3)
88. I need help designing homology arms for CRISPR - (reply: 3)
89. Partial digest XhoI NotI NEB - (reply: 6)
90. removing part from plasmid - (reply: 18)