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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Start codon after promoter - (reply: 1)
62. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
63. Purpose of PolyA in cloning - (reply: 1)
64. Ligation failure? I ran some on a gel - (reply: 6)
65. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
66. Cloning into pVIVO2 vector - (reply: 8)
67. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
68. Cloning worked, but the expression vector is weird - (reply: 6)
69. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
70. site directed mutagenesis - (reply: 3)
71. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
72. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
73. restriction enzymes/sites to avoid - (reply: 4)
74. Using pBABE plasmids for regular transfection - (reply: 1)
75. Ubiquitin promoter cloning - (reply: 1)
76. Cloning two genes in the same plasmid with one promotor - (reply: 4)
77. Sending a TOPO-construct for sequencing? - (reply: 1)
78. Is this counter-productive cloning or not? - (reply: 8)
79. Colony screening - (reply: 1)
80. Failed cloning for 1month - TOPO - (reply: 7)
81. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
82. Which method for DNA purification b4 cloning - (reply: 4)
83. Purification for TOPO - (reply: 1)
84. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
85. Failed TOPO cloning - what happened? - (reply: 9)
86. TOPO cloning after Phusion-PCR - (reply: 1)
87. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
88. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
89. Deleting 7Mbp chromosomal region - (reply: 3)
90. Electrophoresis samples and volumes - (reply: 1)