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Top : New Forum Archives (2009-): : Molecular-Cloning
61. replicating a mammalian expression vector in bacteria - (reply: 3)
62. Cloning of an unknown gene - (reply: 3)
63. cloning problems using in-fusion kit - (reply: 5)
64. Mutation in one chain of homodimer - (reply: 4)
65. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
66. Alternative competent cells for QuikChange kit - (reply: 2)
67. Ligation Mistake - (reply: 4)
68. Cloning - Ligation problem - (reply: 3)
69. How to clone a 2A peptide between two ORFs? - (reply: 2)
70. preparing a vector for transfection - (reply: 2)
71. Ligation of two PCR products - (reply: 1)
72. TA Cloning - (reply: 11)
73. Troubleshootting lentiviral transduction. - (reply: 3)
74. Trouble with PCR using ligation mix as template? - (reply: 3)
75. Suitable ratio of vector and insert in cloning - (reply: 5)
76. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
77. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
78. plasmid repositories - (reply: 2)
79. How to insert PreScission protease into a vector - (reply: 1)
80. Why the insert become shorter after inserting into plasmid? - (reply: 2)
81. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
82. Thermo scientific T4 DNA ligase product help - (reply: 3)
83. Cloning a big insert in a small vector - (reply: 2)
84. Transformation E.Coli help - (reply: 2)
85. Exonucleases that won't harm circular dsDNA - (reply: 1)
86. How to produce transgenic mice? - (reply: 10)
87. No colonies on plate after liagtion - (reply: 7)
88. Question: Can I cut out rb glob PA Terminator - (reply: 1)
89. Low Yield Miniprep - (reply: 5)
90. question about Clonetech Retro-X Tet-on system - (reply: 3)