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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
62. Thermo scientific T4 DNA ligase product help - (reply: 3)
63. Cloning a big insert in a small vector - (reply: 2)
64. Transformation E.Coli help - (reply: 2)
65. Exonucleases that won't harm circular dsDNA - (reply: 1)
66. How to produce transgenic mice? - (reply: 10)
67. No colonies on plate after liagtion - (reply: 7)
68. Question: Can I cut out rb glob PA Terminator - (reply: 1)
69. Low Yield Miniprep - (reply: 5)
70. question about Clonetech Retro-X Tet-on system - (reply: 3)
71. Clonetech Retro-X Tet-on system - (reply: 1)
72. quiaquick gel extraction troubleshooting - (reply: 1)
73. Insert direction in TA cloning - (reply: 2)
74. Transformation in Staphylococcus epidermidis - (reply: 1)
75. Plasmids recombining - (reply: 3)
76. troubleshooting for a simple SDM - (reply: 2)
77. Should I proceed with clone selection? - (reply: 2)
78. Need help in codon optimization... - (reply: 1)
79. transformation - (reply: 2)
80. problem in sequencing of gene - (reply: 3)
81. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
82. Plasmid Methylation - (reply: 3)
83. Problem with BamHI Digestion - (reply: 7)
84. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
85. How to find promoter sequence ... - (reply: 3)
86. Sticky and blunt end ligation in single reaction? - (reply: 2)
87. Restriction double digest - (reply: 1)
88. Choice of AMP 100 versus AMP 50 - (reply: 1)
89. Why are there two origin of replications in plasmids - (reply: 3)
90. How can we expand molecular cloning further? - (reply: 3)