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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Genomic -Lactamase? Blue White screening not working - (reply: 1)
62. direction of the expression units in multi-gene vectors - (reply: 1)
63. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
64. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
65. Measuring pcr fragments in a gel - (reply: 1)
66. gel electrophoresis - (reply: 4)
67. three questions about CRISPR knowout - (reply: 2)
68. Help needed for Ligation/Transformation - (reply: 1)
69. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
70. ClaI restriction enzyme efficency - (reply: 1)
71. Blunt end cloning - (reply: 12)
72. sequantial digest - (reply: 25)
73. Temperature sensitivity of Plasmids - (reply: 1)
74. DMSO in restriction digest - (reply: 1)
75. Molecular cloning - (reply: 5)
76. Nuclease free water for cloning - (reply: 8)
77. Directional cloning 2bps overhang - (reply: 3)
78. I need help designing homology arms for CRISPR - (reply: 3)
79. Partial digest XhoI NotI NEB - (reply: 6)
80. removing part from plasmid - (reply: 18)
81. Start codon after promoter - (reply: 1)
82. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
83. Purpose of PolyA in cloning - (reply: 1)
84. Ligation failure? I ran some on a gel - (reply: 6)
85. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
86. Cloning into pVIVO2 vector - (reply: 8)
87. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
88. Cloning worked, but the expression vector is weird - (reply: 6)
89. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
90. site directed mutagenesis - (reply: 3)