Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
61. Doing a double digest - (reply: 3)
62. Types of igation controls - (reply: 6)
63. Problem with double digest cloning - (reply: 3)
64. Weird results with bacterial transformation - (reply: 11)
65. Site Directed Mutagenesis - no product - (reply: 4)
66. Problems sequencing ligation product - (reply: 5)
67. Faster method to screeen for positive colonies. - (reply: 8)
68. 260/230 ratio, does it matter? - (reply: 4)
69. Double digestion problem with PUCsp vector - (reply: 1)
70. How much plasmid for transformation? - (reply: 8)
71. RE digestion and ligation troubleshooting. - (reply: 1)
72. replicating a mammalian expression vector in bacteria - (reply: 3)
73. Cloning of an unknown gene - (reply: 4)
74. cloning problems using in-fusion kit - (reply: 5)
75. Mutation in one chain of homodimer - (reply: 4)
76. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
77. Alternative competent cells for QuikChange kit - (reply: 2)
78. Ligation Mistake - (reply: 4)
79. Cloning - Ligation problem - (reply: 3)
80. How to clone a 2A peptide between two ORFs? - (reply: 2)
81. preparing a vector for transfection - (reply: 2)
82. Ligation of two PCR products - (reply: 1)
83. TA Cloning - (reply: 11)
84. Troubleshootting lentiviral transduction. - (reply: 3)
85. Trouble with PCR using ligation mix as template? - (reply: 3)
86. Suitable ratio of vector and insert in cloning - (reply: 5)
87. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
88. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
89. plasmid repositories - (reply: 2)
90. How to insert PreScission protease into a vector - (reply: 1)