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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Mutation in one chain of homodimer - (reply: 4)
62. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
63. Alternative competent cells for QuikChange kit - (reply: 2)
64. Ligation Mistake - (reply: 4)
65. Cloning - Ligation problem - (reply: 3)
66. How to clone a 2A peptide between two ORFs? - (reply: 2)
67. preparing a vector for transfection - (reply: 2)
68. Ligation of two PCR products - (reply: 1)
69. TA Cloning - (reply: 11)
70. Troubleshootting lentiviral transduction. - (reply: 3)
71. Trouble with PCR using ligation mix as template? - (reply: 3)
72. Suitable ratio of vector and insert in cloning - (reply: 5)
73. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
74. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
75. plasmid repositories - (reply: 2)
76. How to insert PreScission protease into a vector - (reply: 1)
77. Why the insert become shorter after inserting into plasmid? - (reply: 2)
78. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
79. Thermo scientific T4 DNA ligase product help - (reply: 3)
80. Cloning a big insert in a small vector - (reply: 2)
81. Transformation E.Coli help - (reply: 2)
82. Exonucleases that won't harm circular dsDNA - (reply: 1)
83. How to produce transgenic mice? - (reply: 10)
84. No colonies on plate after liagtion - (reply: 7)
85. Question: Can I cut out rb glob PA Terminator - (reply: 1)
86. Low Yield Miniprep - (reply: 5)
87. question about Clonetech Retro-X Tet-on system - (reply: 3)
88. Clonetech Retro-X Tet-on system - (reply: 1)
89. quiaquick gel extraction troubleshooting - (reply: 1)
90. Insert direction in TA cloning - (reply: 2)