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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Comparing DNA fragments using restriction map - (reply: 1)
62. Doing a double digest - (reply: 3)
63. Types of igation controls - (reply: 6)
64. Problem with double digest cloning - (reply: 3)
65. Weird results with bacterial transformation - (reply: 11)
66. Site Directed Mutagenesis - no product - (reply: 4)
67. Problems sequencing ligation product - (reply: 5)
68. Faster method to screeen for positive colonies. - (reply: 8)
69. 260/230 ratio, does it matter? - (reply: 4)
70. Double digestion problem with PUCsp vector - (reply: 1)
71. How much plasmid for transformation? - (reply: 8)
72. RE digestion and ligation troubleshooting. - (reply: 1)
73. replicating a mammalian expression vector in bacteria - (reply: 3)
74. Cloning of an unknown gene - (reply: 4)
75. cloning problems using in-fusion kit - (reply: 5)
76. Mutation in one chain of homodimer - (reply: 4)
77. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
78. Alternative competent cells for QuikChange kit - (reply: 2)
79. Ligation Mistake - (reply: 4)
80. Cloning - Ligation problem - (reply: 3)
81. How to clone a 2A peptide between two ORFs? - (reply: 2)
82. preparing a vector for transfection - (reply: 2)
83. Ligation of two PCR products - (reply: 1)
84. TA Cloning - (reply: 11)
85. Troubleshootting lentiviral transduction. - (reply: 3)
86. Trouble with PCR using ligation mix as template? - (reply: 3)
87. Suitable ratio of vector and insert in cloning - (reply: 5)
88. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
89. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
90. plasmid repositories - (reply: 2)