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Top : New Forum Archives (2009-): : Molecular-Cloning
61. ratios insert/vector - (reply: 3)
62. Can't isolate plasmid from cloned E.coli - (reply: 4)
63. Genomic DNA quantification using the NanoDrop - (reply: 3)
64. Competent cells with Hanahan's method - (reply: 2)
65. cloning issues - (reply: 3)
66. Is important the direction of IRES-GFP in cloning? - (reply: 1)
67. What are the error-rates of the TA cloning Kits? - (reply: 8)
68. Can mutations be created during cloning? - (reply: 7)
69. confusing with where to put primers and which ordination - (reply: 4)
70. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
71. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
72. RBS - (reply: 1)
73. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
74. Gel for my ligation - (reply: 1)
75. Amplifying from pcr products - (reply: 7)
76. Design the primers - (reply: 10)
77. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
78. How two fragments joint together when doing fusion PCR? - (reply: 4)
79. Working backwards from a construct? - (reply: 1)
80. disappearing bases - (reply: 4)
81. Mutagenesis PCR - (reply: 9)
82. Digest two sites located next to each other - (reply: 5)
83. cDNA Library Construction by using kits and Sequencing - (reply: 1)
84. Looking for software that permutes codons to find new restriction sites - (reply: 4)
85. Transforming dna from agarose gel - (reply: 3)
86. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
87. ccdb toxin - (reply: 15)
88. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
89. annealing oligos - (reply: 3)
90. Does a restriction enzyme cut at a related site? - (reply: 5)