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Top : New Forum Archives (2009-): : Molecular-Cloning
61. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
62. How to find promoter sequence ... - (reply: 3)
63. Sticky and blunt end ligation in single reaction? - (reply: 2)
64. Restriction double digest - (reply: 1)
65. Choice of AMP 100 versus AMP 50 - (reply: 1)
66. Why are there two origin of replications in plasmids - (reply: 3)
67. How can we expand molecular cloning further? - (reply: 3)
68. Subtle change in insert size after cloning - (reply: 6)
69. lactic acid bacteria - cloning - (reply: 2)
70. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
71. All I get is supercoiled DNA... - (reply: 5)
72. Help Quesion - (reply: 1)
73. No colonies after self Circularization ligation - (reply: 2)
74. Clean ligation controls but no insert - (reply: 14)
75. ratios insert/vector - (reply: 3)
76. Can't isolate plasmid from cloned E.coli - (reply: 4)
77. Genomic DNA quantification using the NanoDrop - (reply: 3)
78. Competent cells with Hanahan's method - (reply: 2)
79. cloning issues - (reply: 3)
80. Is important the direction of IRES-GFP in cloning? - (reply: 1)
81. What are the error-rates of the TA cloning Kits? - (reply: 8)
82. Can mutations be created during cloning? - (reply: 7)
83. confusing with where to put primers and which ordination - (reply: 4)
84. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
85. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
86. RBS - (reply: 1)
87. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
88. Gel for my ligation - (reply: 1)
89. Amplifying from pcr products - (reply: 7)
90. Design the primers - (reply: 10)