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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Sending a TOPO-construct for sequencing? - (reply: 1)
92. Is this counter-productive cloning or not? - (reply: 8)
93. Colony screening - (reply: 1)
94. Failed cloning for 1month - TOPO - (reply: 7)
95. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
96. Which method for DNA purification b4 cloning - (reply: 4)
97. Purification for TOPO - (reply: 1)
98. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
99. Failed TOPO cloning - what happened? - (reply: 9)
100. TOPO cloning after Phusion-PCR - (reply: 1)
101. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
102. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
103. Deleting 7Mbp chromosomal region - (reply: 3)
104. Electrophoresis samples and volumes - (reply: 1)
105. Gene cloning with unknown sequence - (reply: 6)
106. Checking for efficieny competent cells - (reply: 1)
107. Troubles with Fusion PCR - (reply: 1)
108. Does this look alright? - (reply: 3)
109. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
110. Cloning pDsRed-Monomer-N1 - (reply: 1)
111. forgot to heat shock - (reply: 6)
112. Ligation left for weekend at room temp - (reply: 4)
113. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
114. Problem in plasmid digestion after transformation - (reply: 4)
115. Lac Promoter activity in P. aeruginosa - (reply: 1)
116. Resctriction analysis - (reply: 5)
117. Please comment on my Cloning Plan - (reply: 1)
118. Gibson/SLIC question - (reply: 1)
119. How to determine where, in the genome, my transgene inserted - (reply: 4)
120. no colonies, ever! - (reply: 5)