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Top : New Forum Archives (2009-): : Molecular-Cloning
91. transformation - (reply: 2)
92. problem in sequencing of gene - (reply: 3)
93. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
94. Plasmid Methylation - (reply: 3)
95. Problem with BamHI Digestion - (reply: 7)
96. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
97. How to find promoter sequence ... - (reply: 3)
98. Sticky and blunt end ligation in single reaction? - (reply: 2)
99. Restriction double digest - (reply: 1)
100. Choice of AMP 100 versus AMP 50 - (reply: 1)
101. Why are there two origin of replications in plasmids - (reply: 3)
102. How can we expand molecular cloning further? - (reply: 3)
103. Subtle change in insert size after cloning - (reply: 6)
104. lactic acid bacteria - cloning - (reply: 2)
105. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
106. All I get is supercoiled DNA... - (reply: 5)
107. Help Quesion - (reply: 1)
108. No colonies after self Circularization ligation - (reply: 2)
109. Clean ligation controls but no insert - (reply: 14)
110. ratios insert/vector - (reply: 3)
111. Can't isolate plasmid from cloned E.coli - (reply: 4)
112. Genomic DNA quantification using the NanoDrop - (reply: 3)
113. Competent cells with Hanahan's method - (reply: 2)
114. cloning issues - (reply: 3)
115. Is important the direction of IRES-GFP in cloning? - (reply: 1)
116. What are the error-rates of the TA cloning Kits? - (reply: 8)
117. Can mutations be created during cloning? - (reply: 7)
118. confusing with where to put primers and which ordination - (reply: 4)
119. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
120. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)