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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
92. ccdb toxin - (reply: 15)
93. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
94. annealing oligos - (reply: 3)
95. Does a restriction enzyme cut at a related site? - (reply: 5)
96. primers for cloning - (reply: 1)
97. Vector-insert ratio - (reply: 6)
98. Cloning contamination - (reply: 3)
99. plasmid construction - (reply: 2)
100. primer design with restriction enzyme - (reply: 1)
101. vector propagation in E coli - (reply: 1)
102. Restriction to remove background - (reply: 4)
103. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
104. Restriction and ligation time interval - (reply: 1)
105. Problem shrna cloning, bad sequencing results - (reply: 1)
106. Lentivirus copy number - (reply: 7)
107. Genomic -Lactamase? Blue White screening not working - (reply: 1)
108. direction of the expression units in multi-gene vectors - (reply: 1)
109. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
110. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
111. Measuring pcr fragments in a gel - (reply: 1)
112. gel electrophoresis - (reply: 4)
113. three questions about CRISPR knowout - (reply: 2)
114. Help needed for Ligation/Transformation - (reply: 1)
115. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
116. ClaI restriction enzyme efficency - (reply: 1)
117. Blunt end cloning - (reply: 12)
118. sequantial digest - (reply: 25)
119. Temperature sensitivity of Plasmids - (reply: 1)
120. DMSO in restriction digest - (reply: 1)