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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
92. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
93. Measuring pcr fragments in a gel - (reply: 1)
94. gel electrophoresis - (reply: 4)
95. three questions about CRISPR knowout - (reply: 2)
96. Help needed for Ligation/Transformation - (reply: 1)
97. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
98. ClaI restriction enzyme efficency - (reply: 1)
99. Blunt end cloning - (reply: 12)
100. sequantial digest - (reply: 25)
101. Temperature sensitivity of Plasmids - (reply: 1)
102. DMSO in restriction digest - (reply: 1)
103. Molecular cloning - (reply: 5)
104. Nuclease free water for cloning - (reply: 8)
105. Directional cloning 2bps overhang - (reply: 3)
106. I need help designing homology arms for CRISPR - (reply: 3)
107. Partial digest XhoI NotI NEB - (reply: 6)
108. removing part from plasmid - (reply: 18)
109. Start codon after promoter - (reply: 1)
110. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
111. Purpose of PolyA in cloning - (reply: 1)
112. Ligation failure? I ran some on a gel - (reply: 6)
113. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
114. Cloning into pVIVO2 vector - (reply: 8)
115. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
116. Cloning worked, but the expression vector is weird - (reply: 6)
117. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
118. site directed mutagenesis - (reply: 3)
119. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
120. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)