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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Plasmid Methylation - (reply: 3)
92. Problem with BamHI Digestion - (reply: 7)
93. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
94. How to find promoter sequence ... - (reply: 3)
95. Sticky and blunt end ligation in single reaction? - (reply: 2)
96. Restriction double digest - (reply: 1)
97. Choice of AMP 100 versus AMP 50 - (reply: 1)
98. Why are there two origin of replications in plasmids - (reply: 3)
99. How can we expand molecular cloning further? - (reply: 3)
100. Subtle change in insert size after cloning - (reply: 6)
101. lactic acid bacteria - cloning - (reply: 2)
102. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
103. All I get is supercoiled DNA... - (reply: 5)
104. Help Quesion - (reply: 1)
105. No colonies after self Circularization ligation - (reply: 2)
106. Clean ligation controls but no insert - (reply: 14)
107. ratios insert/vector - (reply: 3)
108. Can't isolate plasmid from cloned E.coli - (reply: 4)
109. Genomic DNA quantification using the NanoDrop - (reply: 3)
110. Competent cells with Hanahan's method - (reply: 2)
111. cloning issues - (reply: 3)
112. Is important the direction of IRES-GFP in cloning? - (reply: 1)
113. What are the error-rates of the TA cloning Kits? - (reply: 8)
114. Can mutations be created during cloning? - (reply: 7)
115. confusing with where to put primers and which ordination - (reply: 4)
116. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
117. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
118. RBS - (reply: 1)
119. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
120. Gel for my ligation - (reply: 1)