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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
92. Plasmid Methylation - (reply: 3)
93. Problem with BamHI Digestion - (reply: 7)
94. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
95. How to find promoter sequence ... - (reply: 3)
96. Sticky and blunt end ligation in single reaction? - (reply: 2)
97. Restriction double digest - (reply: 1)
98. Choice of AMP 100 versus AMP 50 - (reply: 1)
99. Why are there two origin of replications in plasmids - (reply: 3)
100. How can we expand molecular cloning further? - (reply: 3)
101. Subtle change in insert size after cloning - (reply: 6)
102. lactic acid bacteria - cloning - (reply: 2)
103. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
104. All I get is supercoiled DNA... - (reply: 5)
105. Help Quesion - (reply: 1)
106. No colonies after self Circularization ligation - (reply: 2)
107. Clean ligation controls but no insert - (reply: 14)
108. ratios insert/vector - (reply: 3)
109. Can't isolate plasmid from cloned E.coli - (reply: 4)
110. Genomic DNA quantification using the NanoDrop - (reply: 3)
111. Competent cells with Hanahan's method - (reply: 2)
112. cloning issues - (reply: 3)
113. Is important the direction of IRES-GFP in cloning? - (reply: 1)
114. What are the error-rates of the TA cloning Kits? - (reply: 8)
115. Can mutations be created during cloning? - (reply: 7)
116. confusing with where to put primers and which ordination - (reply: 4)
117. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
118. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
119. RBS - (reply: 1)
120. Ligation: is it possible to ligate two vector backbones together - (reply: 11)