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Top : New Forum Archives (2009-): : Molecular-Cloning
91. annealing oligos - (reply: 3)
92. Does a restriction enzyme cut at a related site? - (reply: 5)
93. primers for cloning - (reply: 1)
94. Vector-insert ratio - (reply: 6)
95. Cloning contamination - (reply: 3)
96. plasmid construction - (reply: 2)
97. primer design with restriction enzyme - (reply: 1)
98. vector propagation in E coli - (reply: 1)
99. Restriction to remove background - (reply: 4)
100. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
101. Restriction and ligation time interval - (reply: 1)
102. Problem shrna cloning, bad sequencing results - (reply: 1)
103. Lentivirus copy number - (reply: 7)
104. Genomic -Lactamase? Blue White screening not working - (reply: 1)
105. direction of the expression units in multi-gene vectors - (reply: 1)
106. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
107. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
108. Measuring pcr fragments in a gel - (reply: 1)
109. gel electrophoresis - (reply: 4)
110. three questions about CRISPR knowout - (reply: 2)
111. Help needed for Ligation/Transformation - (reply: 1)
112. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
113. ClaI restriction enzyme efficency - (reply: 1)
114. Blunt end cloning - (reply: 12)
115. sequantial digest - (reply: 25)
116. Temperature sensitivity of Plasmids - (reply: 1)
117. DMSO in restriction digest - (reply: 1)
118. Molecular cloning - (reply: 5)
119. Nuclease free water for cloning - (reply: 8)
120. Directional cloning 2bps overhang - (reply: 3)