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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Subtle change in insert size after cloning - (reply: 6)
92. lactic acid bacteria - cloning - (reply: 2)
93. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
94. All I get is supercoiled DNA... - (reply: 5)
95. Help Quesion - (reply: 1)
96. No colonies after self Circularization ligation - (reply: 2)
97. Clean ligation controls but no insert - (reply: 14)
98. ratios insert/vector - (reply: 3)
99. Can't isolate plasmid from cloned E.coli - (reply: 4)
100. Genomic DNA quantification using the NanoDrop - (reply: 3)
101. Competent cells with Hanahan's method - (reply: 2)
102. cloning issues - (reply: 3)
103. Is important the direction of IRES-GFP in cloning? - (reply: 1)
104. What are the error-rates of the TA cloning Kits? - (reply: 8)
105. Can mutations be created during cloning? - (reply: 7)
106. confusing with where to put primers and which ordination - (reply: 4)
107. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
108. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
109. RBS - (reply: 1)
110. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
111. Gel for my ligation - (reply: 1)
112. Amplifying from pcr products - (reply: 7)
113. Design the primers - (reply: 10)
114. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
115. How two fragments joint together when doing fusion PCR? - (reply: 4)
116. Working backwards from a construct? - (reply: 1)
117. disappearing bases - (reply: 4)
118. Mutagenesis PCR - (reply: 9)
119. Digest two sites located next to each other - (reply: 5)
120. cDNA Library Construction by using kits and Sequencing - (reply: 1)