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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Purification for TOPO - (reply: 1)
92. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
93. Failed TOPO cloning - what happened? - (reply: 9)
94. TOPO cloning after Phusion-PCR - (reply: 1)
95. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
96. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
97. Deleting 7Mbp chromosomal region - (reply: 3)
98. Electrophoresis samples and volumes - (reply: 1)
99. Gene cloning with unknown sequence - (reply: 6)
100. Checking for efficieny competent cells - (reply: 1)
101. Troubles with Fusion PCR - (reply: 1)
102. Does this look alright? - (reply: 3)
103. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
104. Cloning pDsRed-Monomer-N1 - (reply: 1)
105. forgot to heat shock - (reply: 6)
106. Ligation left for weekend at room temp - (reply: 4)
107. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
108. Problem in plasmid digestion after transformation - (reply: 4)
109. Lac Promoter activity in P. aeruginosa - (reply: 1)
110. Resctriction analysis - (reply: 5)
111. Please comment on my Cloning Plan - (reply: 1)
112. Gibson/SLIC question - (reply: 1)
113. How to determine where, in the genome, my transgene inserted - (reply: 4)
114. no colonies, ever! - (reply: 5)
115. fluorescent tag on membrane protein - (reply: 2)
116. How can addgene sell plasmids with patented technology - (reply: 2)
117. no insert issue - (reply: 9)
118. Considerations on the design of a transgene - (reply: 1)
119. How to obtain Vectors used in articles - (reply: 5)
120. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)