|
91. Incomplete digestion of plasmid with single enzyme - (reply: 1)
92. problem with plasmid digestion - (reply: 5)
93. Difficult Ligation - Details Inside - (reply: 9)
94. pET 32 (+) - (reply: 1)
95. Alternative to pET Vectorsystem - (reply: 2)
96. pEGFP C-1/N-1 Cloning - (reply: 11)
97. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
98. Problems with growing out transformants in liquid LB - (reply: 3)
99. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
100. Ethidiumbromide - (reply: 3)
101. TRYPSIN - (reply: 4)
102. Problem with cloning - what is wrong? - (reply: 6)
103. cloning: band at different site than desired after RE digestion - (reply: 11)
104. GST TAG - (reply: 3)
105. Residues on the N terminal GST Tag - (reply: 1)
106. Where to place the polyA signal? - (reply: 1)
107. identification of target sequence - (reply: 3)
108. cloning pcr insert into plasmid - (reply: 2)
109. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
110. Large quantity heat shock trouble? - (reply: 3)
111. molecular cloning - (reply: 7)
112. Cannot see insert with colony pcr - (reply: 6)
113. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
114. help with a vector - (reply: 3)
115. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
116. Another cloning hell....no transformants - (reply: 1)
117. Agrobacterium LBA4404 transformation - (reply: 1)
118. TOPO TA Cloning Troubleshooting - (reply: 1)
119. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
120. Tricistronic vector - (reply: 1)
92. problem with plasmid digestion - (reply: 5)
93. Difficult Ligation - Details Inside - (reply: 9)
94. pET 32 (+) - (reply: 1)
95. Alternative to pET Vectorsystem - (reply: 2)
96. pEGFP C-1/N-1 Cloning - (reply: 11)
97. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
98. Problems with growing out transformants in liquid LB - (reply: 3)
99. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
100. Ethidiumbromide - (reply: 3)
101. TRYPSIN - (reply: 4)
102. Problem with cloning - what is wrong? - (reply: 6)
103. cloning: band at different site than desired after RE digestion - (reply: 11)
104. GST TAG - (reply: 3)
105. Residues on the N terminal GST Tag - (reply: 1)
106. Where to place the polyA signal? - (reply: 1)
107. identification of target sequence - (reply: 3)
108. cloning pcr insert into plasmid - (reply: 2)
109. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
110. Large quantity heat shock trouble? - (reply: 3)
111. molecular cloning - (reply: 7)
112. Cannot see insert with colony pcr - (reply: 6)
113. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
114. help with a vector - (reply: 3)
115. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
116. Another cloning hell....no transformants - (reply: 1)
117. Agrobacterium LBA4404 transformation - (reply: 1)
118. TOPO TA Cloning Troubleshooting - (reply: 1)
119. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
120. Tricistronic vector - (reply: 1)