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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Insert direction in TA cloning - (reply: 2)
92. Transformation in Staphylococcus epidermidis - (reply: 1)
93. Plasmids recombining - (reply: 3)
94. troubleshooting for a simple SDM - (reply: 2)
95. Should I proceed with clone selection? - (reply: 2)
96. Need help in codon optimization... - (reply: 1)
97. transformation - (reply: 2)
98. problem in sequencing of gene - (reply: 3)
99. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
100. Plasmid Methylation - (reply: 3)
101. Problem with BamHI Digestion - (reply: 7)
102. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
103. How to find promoter sequence ... - (reply: 3)
104. Sticky and blunt end ligation in single reaction? - (reply: 2)
105. Restriction double digest - (reply: 1)
106. Choice of AMP 100 versus AMP 50 - (reply: 1)
107. Why are there two origin of replications in plasmids - (reply: 3)
108. How can we expand molecular cloning further? - (reply: 3)
109. Subtle change in insert size after cloning - (reply: 6)
110. lactic acid bacteria - cloning - (reply: 2)
111. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
112. All I get is supercoiled DNA... - (reply: 5)
113. Help Quesion - (reply: 1)
114. No colonies after self Circularization ligation - (reply: 2)
115. Clean ligation controls but no insert - (reply: 14)
116. ratios insert/vector - (reply: 3)
117. Can't isolate plasmid from cloned E.coli - (reply: 4)
118. Genomic DNA quantification using the NanoDrop - (reply: 3)
119. Competent cells with Hanahan's method - (reply: 2)
120. cloning issues - (reply: 3)