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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Plasmids recombining - (reply: 3)
92. troubleshooting for a simple SDM - (reply: 2)
93. Should I proceed with clone selection? - (reply: 2)
94. Need help in codon optimization... - (reply: 1)
95. transformation - (reply: 2)
96. problem in sequencing of gene - (reply: 3)
97. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
98. Plasmid Methylation - (reply: 3)
99. Problem with BamHI Digestion - (reply: 7)
100. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
101. How to find promoter sequence ... - (reply: 3)
102. Sticky and blunt end ligation in single reaction? - (reply: 2)
103. Restriction double digest - (reply: 1)
104. Choice of AMP 100 versus AMP 50 - (reply: 1)
105. Why are there two origin of replications in plasmids - (reply: 3)
106. How can we expand molecular cloning further? - (reply: 3)
107. Subtle change in insert size after cloning - (reply: 6)
108. lactic acid bacteria - cloning - (reply: 2)
109. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
110. All I get is supercoiled DNA... - (reply: 5)
111. Help Quesion - (reply: 1)
112. No colonies after self Circularization ligation - (reply: 2)
113. Clean ligation controls but no insert - (reply: 14)
114. ratios insert/vector - (reply: 3)
115. Can't isolate plasmid from cloned E.coli - (reply: 4)
116. Genomic DNA quantification using the NanoDrop - (reply: 3)
117. Competent cells with Hanahan's method - (reply: 2)
118. cloning issues - (reply: 3)
119. Is important the direction of IRES-GFP in cloning? - (reply: 1)
120. What are the error-rates of the TA cloning Kits? - (reply: 8)