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Top : New Forum Archives (2009-): : Molecular-Cloning
91. Why the insert become shorter after inserting into plasmid? - (reply: 2)
92. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
93. Thermo scientific T4 DNA ligase product help - (reply: 3)
94. Cloning a big insert in a small vector - (reply: 2)
95. Transformation E.Coli help - (reply: 2)
96. Exonucleases that won't harm circular dsDNA - (reply: 1)
97. How to produce transgenic mice? - (reply: 10)
98. No colonies on plate after liagtion - (reply: 7)
99. Question: Can I cut out rb glob PA Terminator - (reply: 1)
100. Low Yield Miniprep - (reply: 5)
101. question about Clonetech Retro-X Tet-on system - (reply: 3)
102. Clonetech Retro-X Tet-on system - (reply: 1)
103. quiaquick gel extraction troubleshooting - (reply: 1)
104. Insert direction in TA cloning - (reply: 2)
105. Transformation in Staphylococcus epidermidis - (reply: 1)
106. Plasmids recombining - (reply: 3)
107. troubleshooting for a simple SDM - (reply: 2)
108. Should I proceed with clone selection? - (reply: 2)
109. Need help in codon optimization... - (reply: 1)
110. transformation - (reply: 2)
111. problem in sequencing of gene - (reply: 3)
112. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
113. Plasmid Methylation - (reply: 3)
114. Problem with BamHI Digestion - (reply: 7)
115. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
116. How to find promoter sequence ... - (reply: 3)
117. Sticky and blunt end ligation in single reaction? - (reply: 2)
118. Restriction double digest - (reply: 1)
119. Choice of AMP 100 versus AMP 50 - (reply: 1)
120. Why are there two origin of replications in plasmids - (reply: 3)