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Top : New Forum Archives (2009-): : Molecular-Cloning
91. I need help designing homology arms for CRISPR - (reply: 3)
92. Partial digest XhoI NotI NEB - (reply: 6)
93. removing part from plasmid - (reply: 18)
94. Start codon after promoter - (reply: 1)
95. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
96. Purpose of PolyA in cloning - (reply: 1)
97. Ligation failure? I ran some on a gel - (reply: 6)
98. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
99. Cloning into pVIVO2 vector - (reply: 8)
100. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
101. Cloning worked, but the expression vector is weird - (reply: 6)
102. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
103. site directed mutagenesis - (reply: 3)
104. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
105. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
106. restriction enzymes/sites to avoid - (reply: 4)
107. Using pBABE plasmids for regular transfection - (reply: 1)
108. Ubiquitin promoter cloning - (reply: 1)
109. Cloning two genes in the same plasmid with one promotor - (reply: 4)
110. Sending a TOPO-construct for sequencing? - (reply: 1)
111. Is this counter-productive cloning or not? - (reply: 8)
112. Colony screening - (reply: 1)
113. Failed cloning for 1month - TOPO - (reply: 7)
114. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
115. Which method for DNA purification b4 cloning - (reply: 4)
116. Purification for TOPO - (reply: 1)
117. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
118. Failed TOPO cloning - what happened? - (reply: 9)
119. TOPO cloning after Phusion-PCR - (reply: 1)
120. Gateway cloning-modifications to "entry" plasmid - (reply: 4)