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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Is important the direction of IRES-GFP in cloning? - (reply: 1)
122. What are the error-rates of the TA cloning Kits? - (reply: 8)
123. Can mutations be created during cloning? - (reply: 7)
124. confusing with where to put primers and which ordination - (reply: 4)
125. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
126. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
127. RBS - (reply: 1)
128. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
129. Gel for my ligation - (reply: 1)
130. Amplifying from pcr products - (reply: 7)
131. Design the primers - (reply: 10)
132. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
133. How two fragments joint together when doing fusion PCR? - (reply: 4)
134. Working backwards from a construct? - (reply: 1)
135. disappearing bases - (reply: 4)
136. Mutagenesis PCR - (reply: 9)
137. Digest two sites located next to each other - (reply: 5)
138. cDNA Library Construction by using kits and Sequencing - (reply: 1)
139. Looking for software that permutes codons to find new restriction sites - (reply: 4)
140. Transforming dna from agarose gel - (reply: 3)
141. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
142. ccdb toxin - (reply: 15)
143. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
144. annealing oligos - (reply: 3)
145. Does a restriction enzyme cut at a related site? - (reply: 5)
146. primers for cloning - (reply: 1)
147. Vector-insert ratio - (reply: 6)
148. Cloning contamination - (reply: 3)
149. plasmid construction - (reply: 2)
150. primer design with restriction enzyme - (reply: 1)