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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
122. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
123. RBS - (reply: 1)
124. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
125. Gel for my ligation - (reply: 1)
126. Amplifying from pcr products - (reply: 7)
127. Design the primers - (reply: 10)
128. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
129. How two fragments joint together when doing fusion PCR? - (reply: 4)
130. Working backwards from a construct? - (reply: 1)
131. disappearing bases - (reply: 4)
132. Mutagenesis PCR - (reply: 9)
133. Digest two sites located next to each other - (reply: 5)
134. cDNA Library Construction by using kits and Sequencing - (reply: 1)
135. Looking for software that permutes codons to find new restriction sites - (reply: 4)
136. Transforming dna from agarose gel - (reply: 3)
137. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
138. ccdb toxin - (reply: 15)
139. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
140. annealing oligos - (reply: 3)
141. Does a restriction enzyme cut at a related site? - (reply: 5)
142. primers for cloning - (reply: 1)
143. Vector-insert ratio - (reply: 6)
144. Cloning contamination - (reply: 3)
145. plasmid construction - (reply: 2)
146. primer design with restriction enzyme - (reply: 1)
147. vector propagation in E coli - (reply: 1)
148. Restriction to remove background - (reply: 4)
149. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
150. Restriction and ligation time interval - (reply: 1)