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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Why are there two origin of replications in plasmids - (reply: 3)
122. How can we expand molecular cloning further? - (reply: 3)
123. Subtle change in insert size after cloning - (reply: 6)
124. lactic acid bacteria - cloning - (reply: 2)
125. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
126. All I get is supercoiled DNA... - (reply: 5)
127. Help Quesion - (reply: 1)
128. No colonies after self Circularization ligation - (reply: 2)
129. Clean ligation controls but no insert - (reply: 14)
130. ratios insert/vector - (reply: 3)
131. Can't isolate plasmid from cloned E.coli - (reply: 4)
132. Genomic DNA quantification using the NanoDrop - (reply: 3)
133. Competent cells with Hanahan's method - (reply: 2)
134. cloning issues - (reply: 3)
135. Is important the direction of IRES-GFP in cloning? - (reply: 1)
136. What are the error-rates of the TA cloning Kits? - (reply: 8)
137. Can mutations be created during cloning? - (reply: 7)
138. confusing with where to put primers and which ordination - (reply: 4)
139. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
140. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
141. RBS - (reply: 1)
142. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
143. Gel for my ligation - (reply: 1)
144. Amplifying from pcr products - (reply: 7)
145. Design the primers - (reply: 10)
146. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
147. How two fragments joint together when doing fusion PCR? - (reply: 4)
148. Working backwards from a construct? - (reply: 1)
149. disappearing bases - (reply: 4)
150. Mutagenesis PCR - (reply: 9)