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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Which method for DNA purification b4 cloning - (reply: 4)
122. Purification for TOPO - (reply: 1)
123. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
124. Failed TOPO cloning - what happened? - (reply: 9)
125. TOPO cloning after Phusion-PCR - (reply: 1)
126. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
127. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
128. Deleting 7Mbp chromosomal region - (reply: 3)
129. Electrophoresis samples and volumes - (reply: 1)
130. Gene cloning with unknown sequence - (reply: 6)
131. Checking for efficieny competent cells - (reply: 1)
132. Troubles with Fusion PCR - (reply: 1)
133. Does this look alright? - (reply: 3)
134. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
135. Cloning pDsRed-Monomer-N1 - (reply: 1)
136. forgot to heat shock - (reply: 6)
137. Ligation left for weekend at room temp - (reply: 4)
138. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
139. Problem in plasmid digestion after transformation - (reply: 4)
140. Lac Promoter activity in P. aeruginosa - (reply: 1)
141. Resctriction analysis - (reply: 5)
142. Please comment on my Cloning Plan - (reply: 1)
143. Gibson/SLIC question - (reply: 1)
144. How to determine where, in the genome, my transgene inserted - (reply: 4)
145. no colonies, ever! - (reply: 5)
146. fluorescent tag on membrane protein - (reply: 2)
147. How can addgene sell plasmids with patented technology - (reply: 2)
148. no insert issue - (reply: 9)
149. Considerations on the design of a transgene - (reply: 1)
150. How to obtain Vectors used in articles - (reply: 5)