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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Measuring pcr fragments in a gel - (reply: 1)
122. gel electrophoresis - (reply: 4)
123. three questions about CRISPR knowout - (reply: 2)
124. Help needed for Ligation/Transformation - (reply: 1)
125. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
126. ClaI restriction enzyme efficency - (reply: 1)
127. Blunt end cloning - (reply: 12)
128. sequantial digest - (reply: 25)
129. Temperature sensitivity of Plasmids - (reply: 1)
130. DMSO in restriction digest - (reply: 1)
131. Molecular cloning - (reply: 5)
132. Nuclease free water for cloning - (reply: 8)
133. Directional cloning 2bps overhang - (reply: 3)
134. I need help designing homology arms for CRISPR - (reply: 3)
135. Partial digest XhoI NotI NEB - (reply: 6)
136. removing part from plasmid - (reply: 18)
137. Start codon after promoter - (reply: 1)
138. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
139. Purpose of PolyA in cloning - (reply: 1)
140. Ligation failure? I ran some on a gel - (reply: 6)
141. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
142. Cloning into pVIVO2 vector - (reply: 8)
143. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
144. Cloning worked, but the expression vector is weird - (reply: 6)
145. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
146. site directed mutagenesis - (reply: 3)
147. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
148. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
149. restriction enzymes/sites to avoid - (reply: 4)
150. Using pBABE plasmids for regular transfection - (reply: 1)