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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
122. Gel for my ligation - (reply: 1)
123. Amplifying from pcr products - (reply: 7)
124. Design the primers - (reply: 10)
125. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
126. How two fragments joint together when doing fusion PCR? - (reply: 4)
127. Working backwards from a construct? - (reply: 1)
128. disappearing bases - (reply: 4)
129. Mutagenesis PCR - (reply: 9)
130. Digest two sites located next to each other - (reply: 5)
131. cDNA Library Construction by using kits and Sequencing - (reply: 1)
132. Looking for software that permutes codons to find new restriction sites - (reply: 4)
133. Transforming dna from agarose gel - (reply: 3)
134. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
135. ccdb toxin - (reply: 15)
136. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
137. annealing oligos - (reply: 3)
138. Does a restriction enzyme cut at a related site? - (reply: 5)
139. primers for cloning - (reply: 1)
140. Vector-insert ratio - (reply: 6)
141. Cloning contamination - (reply: 3)
142. plasmid construction - (reply: 2)
143. primer design with restriction enzyme - (reply: 1)
144. vector propagation in E coli - (reply: 1)
145. Restriction to remove background - (reply: 4)
146. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
147. Restriction and ligation time interval - (reply: 1)
148. Problem shrna cloning, bad sequencing results - (reply: 1)
149. Lentivirus copy number - (reply: 7)
150. Genomic -Lactamase? Blue White screening not working - (reply: 1)