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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
122. How to find promoter sequence ... - (reply: 3)
123. Sticky and blunt end ligation in single reaction? - (reply: 2)
124. Restriction double digest - (reply: 1)
125. Choice of AMP 100 versus AMP 50 - (reply: 1)
126. Why are there two origin of replications in plasmids - (reply: 3)
127. How can we expand molecular cloning further? - (reply: 3)
128. Subtle change in insert size after cloning - (reply: 6)
129. lactic acid bacteria - cloning - (reply: 2)
130. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
131. All I get is supercoiled DNA... - (reply: 5)
132. Help Quesion - (reply: 1)
133. No colonies after self Circularization ligation - (reply: 2)
134. Clean ligation controls but no insert - (reply: 14)
135. ratios insert/vector - (reply: 3)
136. Can't isolate plasmid from cloned E.coli - (reply: 4)
137. Genomic DNA quantification using the NanoDrop - (reply: 3)
138. Competent cells with Hanahan's method - (reply: 2)
139. cloning issues - (reply: 3)
140. Is important the direction of IRES-GFP in cloning? - (reply: 1)
141. What are the error-rates of the TA cloning Kits? - (reply: 8)
142. Can mutations be created during cloning? - (reply: 7)
143. confusing with where to put primers and which ordination - (reply: 4)
144. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
145. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
146. RBS - (reply: 1)
147. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
148. Gel for my ligation - (reply: 1)
149. Amplifying from pcr products - (reply: 7)
150. Design the primers - (reply: 10)