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Top : New Forum Archives (2009-): : Molecular-Cloning
121. lactic acid bacteria - cloning - (reply: 2)
122. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
123. All I get is supercoiled DNA... - (reply: 5)
124. Help Quesion - (reply: 1)
125. No colonies after self Circularization ligation - (reply: 2)
126. Clean ligation controls but no insert - (reply: 14)
127. ratios insert/vector - (reply: 3)
128. Can't isolate plasmid from cloned E.coli - (reply: 4)
129. Genomic DNA quantification using the NanoDrop - (reply: 3)
130. Competent cells with Hanahan's method - (reply: 2)
131. cloning issues - (reply: 3)
132. Is important the direction of IRES-GFP in cloning? - (reply: 1)
133. What are the error-rates of the TA cloning Kits? - (reply: 8)
134. Can mutations be created during cloning? - (reply: 7)
135. confusing with where to put primers and which ordination - (reply: 4)
136. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
137. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
138. RBS - (reply: 1)
139. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
140. Gel for my ligation - (reply: 1)
141. Amplifying from pcr products - (reply: 7)
142. Design the primers - (reply: 10)
143. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
144. How two fragments joint together when doing fusion PCR? - (reply: 4)
145. Working backwards from a construct? - (reply: 1)
146. disappearing bases - (reply: 4)
147. Mutagenesis PCR - (reply: 9)
148. Digest two sites located next to each other - (reply: 5)
149. cDNA Library Construction by using kits and Sequencing - (reply: 1)
150. Looking for software that permutes codons to find new restriction sites - (reply: 4)