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Top : New Forum Archives (2009-): : Molecular-Cloning
121. How to find promoter sequence ... - (reply: 3)
122. Sticky and blunt end ligation in single reaction? - (reply: 2)
123. Restriction double digest - (reply: 1)
124. Choice of AMP 100 versus AMP 50 - (reply: 1)
125. Why are there two origin of replications in plasmids - (reply: 3)
126. How can we expand molecular cloning further? - (reply: 3)
127. Subtle change in insert size after cloning - (reply: 6)
128. lactic acid bacteria - cloning - (reply: 2)
129. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
130. All I get is supercoiled DNA... - (reply: 5)
131. Help Quesion - (reply: 1)
132. No colonies after self Circularization ligation - (reply: 2)
133. Clean ligation controls but no insert - (reply: 14)
134. ratios insert/vector - (reply: 3)
135. Can't isolate plasmid from cloned E.coli - (reply: 4)
136. Genomic DNA quantification using the NanoDrop - (reply: 3)
137. Competent cells with Hanahan's method - (reply: 2)
138. cloning issues - (reply: 3)
139. Is important the direction of IRES-GFP in cloning? - (reply: 1)
140. What are the error-rates of the TA cloning Kits? - (reply: 8)
141. Can mutations be created during cloning? - (reply: 7)
142. confusing with where to put primers and which ordination - (reply: 4)
143. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
144. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
145. RBS - (reply: 1)
146. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
147. Gel for my ligation - (reply: 1)
148. Amplifying from pcr products - (reply: 7)
149. Design the primers - (reply: 10)
150. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)