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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
122. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
123. Measuring pcr fragments in a gel - (reply: 1)
124. gel electrophoresis - (reply: 4)
125. three questions about CRISPR knowout - (reply: 2)
126. Help needed for Ligation/Transformation - (reply: 1)
127. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
128. ClaI restriction enzyme efficency - (reply: 1)
129. Blunt end cloning - (reply: 12)
130. sequantial digest - (reply: 25)
131. Temperature sensitivity of Plasmids - (reply: 1)
132. DMSO in restriction digest - (reply: 1)
133. Molecular cloning - (reply: 5)
134. Nuclease free water for cloning - (reply: 8)
135. Directional cloning 2bps overhang - (reply: 3)
136. I need help designing homology arms for CRISPR - (reply: 3)
137. Partial digest XhoI NotI NEB - (reply: 6)
138. removing part from plasmid - (reply: 18)
139. Start codon after promoter - (reply: 1)
140. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
141. Purpose of PolyA in cloning - (reply: 1)
142. Ligation failure? I ran some on a gel - (reply: 6)
143. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
144. Cloning into pVIVO2 vector - (reply: 8)
145. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
146. Cloning worked, but the expression vector is weird - (reply: 6)
147. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
148. site directed mutagenesis - (reply: 3)
149. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
150. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)