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Top : New Forum Archives (2009-): : Molecular-Cloning
121. cloning issues - (reply: 3)
122. Is important the direction of IRES-GFP in cloning? - (reply: 1)
123. What are the error-rates of the TA cloning Kits? - (reply: 8)
124. Can mutations be created during cloning? - (reply: 7)
125. confusing with where to put primers and which ordination - (reply: 4)
126. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
127. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
128. RBS - (reply: 1)
129. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
130. Gel for my ligation - (reply: 1)
131. Amplifying from pcr products - (reply: 7)
132. Design the primers - (reply: 10)
133. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
134. How two fragments joint together when doing fusion PCR? - (reply: 4)
135. Working backwards from a construct? - (reply: 1)
136. disappearing bases - (reply: 4)
137. Mutagenesis PCR - (reply: 9)
138. Digest two sites located next to each other - (reply: 5)
139. cDNA Library Construction by using kits and Sequencing - (reply: 1)
140. Looking for software that permutes codons to find new restriction sites - (reply: 4)
141. Transforming dna from agarose gel - (reply: 3)
142. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
143. ccdb toxin - (reply: 15)
144. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
145. annealing oligos - (reply: 3)
146. Does a restriction enzyme cut at a related site? - (reply: 5)
147. primers for cloning - (reply: 1)
148. Vector-insert ratio - (reply: 6)
149. Cloning contamination - (reply: 3)
150. plasmid construction - (reply: 2)