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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Lac Promoter activity in P. aeruginosa - (reply: 1)
122. Resctriction analysis - (reply: 5)
123. Please comment on my Cloning Plan - (reply: 1)
124. Gibson/SLIC question - (reply: 1)
125. How to determine where, in the genome, my transgene inserted - (reply: 4)
126. no colonies, ever! - (reply: 5)
127. fluorescent tag on membrane protein - (reply: 2)
128. How can addgene sell plasmids with patented technology - (reply: 2)
129. no insert issue - (reply: 9)
130. Considerations on the design of a transgene - (reply: 1)
131. How to obtain Vectors used in articles - (reply: 5)
132. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
133. How efficient is the 2A sequence - (reply: 2)
134. Transformation keeps failing - (reply: 2)
135. High background issue - (reply: 5)
136. Pcr primers - (reply: 7)
137. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
138. Gateway vectors - (reply: 3)
139. question on gateway - (reply: 1)
140. Cloning problem still not solved - (reply: 2)
141. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
142. Insert 30nt at C-terminal - (reply: 1)
143. How to store a ligation reaction over the weekend? - (reply: 3)
144. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
145. How homologous do homology arms need to be? - (reply: 3)
146. cloning pEGFP-N1 - (reply: 1)
147. Loss of DNA after restriction digestion cleanup - (reply: 2)
148. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
149. separating mixture of plamids - (reply: 7)
150. Kozak sequence and mammalian expression vector - (reply: 3)