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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Can mutations be created during cloning? - (reply: 7)
122. confusing with where to put primers and which ordination - (reply: 4)
123. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
124. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
125. RBS - (reply: 1)
126. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
127. Gel for my ligation - (reply: 1)
128. Amplifying from pcr products - (reply: 7)
129. Design the primers - (reply: 10)
130. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
131. How two fragments joint together when doing fusion PCR? - (reply: 4)
132. Working backwards from a construct? - (reply: 1)
133. disappearing bases - (reply: 4)
134. Mutagenesis PCR - (reply: 9)
135. Digest two sites located next to each other - (reply: 5)
136. cDNA Library Construction by using kits and Sequencing - (reply: 1)
137. Looking for software that permutes codons to find new restriction sites - (reply: 4)
138. Transforming dna from agarose gel - (reply: 3)
139. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
140. ccdb toxin - (reply: 15)
141. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
142. annealing oligos - (reply: 3)
143. Does a restriction enzyme cut at a related site? - (reply: 5)
144. primers for cloning - (reply: 1)
145. Vector-insert ratio - (reply: 6)
146. Cloning contamination - (reply: 3)
147. plasmid construction - (reply: 2)
148. primer design with restriction enzyme - (reply: 1)
149. vector propagation in E coli - (reply: 1)
150. Restriction to remove background - (reply: 4)