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121. Another cloning hell....no transformants - (reply: 1)
122. Agrobacterium LBA4404 transformation - (reply: 1)
123. TOPO TA Cloning Troubleshooting - (reply: 1)
124. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
125. Tricistronic vector - (reply: 1)
126. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
127. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
128. Directions of genes expression illustrations - (reply: 5)
129. Plasmid DNA at RT for a few days - (reply: 4)
130. TOPO TA Cloning - (reply: 1)
131. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
132. No insert in subcloning - (reply: 3)
133. Digest does not confirm presence of fragment - (reply: 5)
134. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
135. pGL3 Promoter replacing TSS bases - (reply: 1)
136. lipofectamine 2000:DNA complex stability - (reply: 2)
137. Problems with Ecoli glycerol stock - (reply: 6)
138. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
139. CIAP dilution - (reply: 5)
140. Problems in RNAi construction by using pUC19 - (reply: 3)
141. Unable to clone in E. coli cells - (reply: 3)
142. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
143. plasmid rearrangement or sabotage - (reply: 2)
144. pLDR20 Vector - (reply: 1)
145. surprising behavior of transformants needs immediate advice! - (reply: 8)
146. cloning antisense promoter in pGL3Basic - (reply: 2)
147. PhiX174 DNA - (reply: 1)
148. Competent bacterial cells - (reply: 2)
149. Replacing puramycin in a pLKO1.puro - (reply: 1)
150. colony cracking for screening of positive clones - (reply: 2)
122. Agrobacterium LBA4404 transformation - (reply: 1)
123. TOPO TA Cloning Troubleshooting - (reply: 1)
124. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
125. Tricistronic vector - (reply: 1)
126. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
127. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
128. Directions of genes expression illustrations - (reply: 5)
129. Plasmid DNA at RT for a few days - (reply: 4)
130. TOPO TA Cloning - (reply: 1)
131. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
132. No insert in subcloning - (reply: 3)
133. Digest does not confirm presence of fragment - (reply: 5)
134. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
135. pGL3 Promoter replacing TSS bases - (reply: 1)
136. lipofectamine 2000:DNA complex stability - (reply: 2)
137. Problems with Ecoli glycerol stock - (reply: 6)
138. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
139. CIAP dilution - (reply: 5)
140. Problems in RNAi construction by using pUC19 - (reply: 3)
141. Unable to clone in E. coli cells - (reply: 3)
142. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
143. plasmid rearrangement or sabotage - (reply: 2)
144. pLDR20 Vector - (reply: 1)
145. surprising behavior of transformants needs immediate advice! - (reply: 8)
146. cloning antisense promoter in pGL3Basic - (reply: 2)
147. PhiX174 DNA - (reply: 1)
148. Competent bacterial cells - (reply: 2)
149. Replacing puramycin in a pLKO1.puro - (reply: 1)
150. colony cracking for screening of positive clones - (reply: 2)