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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Restriction double digest - (reply: 1)
122. Choice of AMP 100 versus AMP 50 - (reply: 1)
123. Why are there two origin of replications in plasmids - (reply: 3)
124. How can we expand molecular cloning further? - (reply: 3)
125. Subtle change in insert size after cloning - (reply: 6)
126. lactic acid bacteria - cloning - (reply: 2)
127. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
128. All I get is supercoiled DNA... - (reply: 5)
129. Help Quesion - (reply: 1)
130. No colonies after self Circularization ligation - (reply: 2)
131. Clean ligation controls but no insert - (reply: 14)
132. ratios insert/vector - (reply: 3)
133. Can't isolate plasmid from cloned E.coli - (reply: 4)
134. Genomic DNA quantification using the NanoDrop - (reply: 3)
135. Competent cells with Hanahan's method - (reply: 2)
136. cloning issues - (reply: 3)
137. Is important the direction of IRES-GFP in cloning? - (reply: 1)
138. What are the error-rates of the TA cloning Kits? - (reply: 8)
139. Can mutations be created during cloning? - (reply: 7)
140. confusing with where to put primers and which ordination - (reply: 4)
141. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
142. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
143. RBS - (reply: 1)
144. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
145. Gel for my ligation - (reply: 1)
146. Amplifying from pcr products - (reply: 7)
147. Design the primers - (reply: 10)
148. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
149. How two fragments joint together when doing fusion PCR? - (reply: 4)
150. Working backwards from a construct? - (reply: 1)