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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
122. Insert 30nt at C-terminal - (reply: 1)
123. How to store a ligation reaction over the weekend? - (reply: 3)
124. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
125. How homologous do homology arms need to be? - (reply: 3)
126. cloning pEGFP-N1 - (reply: 1)
127. Loss of DNA after restriction digestion cleanup - (reply: 2)
128. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
129. separating mixture of plamids - (reply: 7)
130. Kozak sequence and mammalian expression vector - (reply: 3)
131. GFP vector - (reply: 1)
132. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
133. no colony - (reply: 3)
134. Cloning beginner and clueless! - (reply: 1)
135. Which epitope tag to use for ChIP? - (reply: 1)
136. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
137. Ligation-transformation...Never faced such problem ever - (reply: 6)
138. Can multiple genes follow only one promoter? - (reply: 1)
139. Restriction Digest Question - (reply: 4)
140. Problem with Large vector & very small insert - (reply: 1)
141. Storage life of plasmid DNA in water - (reply: 5)
142. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
143. Site-directed mutagenesis failed - (reply: 20)
144. Insert & vector too dilute to perform ligation - (reply: 3)
145. Cloning Problems - (reply: 2)
146. Suitable templates for in vitro transcription - (reply: 1)
147. Different ways to ensure the successful cloning? - (reply: 2)
148. Plasmid DNA digestion problem - (reply: 3)
149. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
150. Single codon insert - (reply: 3)