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Top : New Forum Archives (2009-): : Molecular-Cloning
121. restriction enzymes/sites to avoid - (reply: 4)
122. Using pBABE plasmids for regular transfection - (reply: 1)
123. Ubiquitin promoter cloning - (reply: 1)
124. Cloning two genes in the same plasmid with one promotor - (reply: 4)
125. Sending a TOPO-construct for sequencing? - (reply: 1)
126. Is this counter-productive cloning or not? - (reply: 8)
127. Colony screening - (reply: 1)
128. Failed cloning for 1month - TOPO - (reply: 7)
129. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
130. Which method for DNA purification b4 cloning - (reply: 4)
131. Purification for TOPO - (reply: 1)
132. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
133. Failed TOPO cloning - what happened? - (reply: 9)
134. TOPO cloning after Phusion-PCR - (reply: 1)
135. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
136. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
137. Deleting 7Mbp chromosomal region - (reply: 3)
138. Electrophoresis samples and volumes - (reply: 1)
139. Gene cloning with unknown sequence - (reply: 6)
140. Checking for efficieny competent cells - (reply: 1)
141. Troubles with Fusion PCR - (reply: 1)
142. Does this look alright? - (reply: 3)
143. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
144. Cloning pDsRed-Monomer-N1 - (reply: 1)
145. forgot to heat shock - (reply: 6)
146. Ligation left for weekend at room temp - (reply: 4)
147. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
148. Problem in plasmid digestion after transformation - (reply: 4)
149. Lac Promoter activity in P. aeruginosa - (reply: 1)
150. Resctriction analysis - (reply: 5)