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Top : New Forum Archives (2009-): : Molecular-Cloning
121. What are the error-rates of the TA cloning Kits? - (reply: 8)
122. Can mutations be created during cloning? - (reply: 7)
123. confusing with where to put primers and which ordination - (reply: 4)
124. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
125. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
126. RBS - (reply: 1)
127. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
128. Gel for my ligation - (reply: 1)
129. Amplifying from pcr products - (reply: 7)
130. Design the primers - (reply: 10)
131. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
132. How two fragments joint together when doing fusion PCR? - (reply: 4)
133. Working backwards from a construct? - (reply: 1)
134. disappearing bases - (reply: 4)
135. Mutagenesis PCR - (reply: 9)
136. Digest two sites located next to each other - (reply: 5)
137. cDNA Library Construction by using kits and Sequencing - (reply: 1)
138. Looking for software that permutes codons to find new restriction sites - (reply: 4)
139. Transforming dna from agarose gel - (reply: 3)
140. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
141. ccdb toxin - (reply: 15)
142. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
143. annealing oligos - (reply: 3)
144. Does a restriction enzyme cut at a related site? - (reply: 5)
145. primers for cloning - (reply: 1)
146. Vector-insert ratio - (reply: 6)
147. Cloning contamination - (reply: 3)
148. plasmid construction - (reply: 2)
149. primer design with restriction enzyme - (reply: 1)
150. vector propagation in E coli - (reply: 1)