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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Amplifying from pcr products - (reply: 7)
122. Design the primers - (reply: 10)
123. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
124. How two fragments joint together when doing fusion PCR? - (reply: 4)
125. Working backwards from a construct? - (reply: 1)
126. disappearing bases - (reply: 4)
127. Mutagenesis PCR - (reply: 9)
128. Digest two sites located next to each other - (reply: 5)
129. cDNA Library Construction by using kits and Sequencing - (reply: 1)
130. Looking for software that permutes codons to find new restriction sites - (reply: 4)
131. Transforming dna from agarose gel - (reply: 3)
132. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
133. ccdb toxin - (reply: 15)
134. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
135. annealing oligos - (reply: 3)
136. Does a restriction enzyme cut at a related site? - (reply: 5)
137. primers for cloning - (reply: 1)
138. Vector-insert ratio - (reply: 6)
139. Cloning contamination - (reply: 3)
140. plasmid construction - (reply: 2)
141. primer design with restriction enzyme - (reply: 1)
142. vector propagation in E coli - (reply: 1)
143. Restriction to remove background - (reply: 4)
144. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
145. Restriction and ligation time interval - (reply: 1)
146. Problem shrna cloning, bad sequencing results - (reply: 1)
147. Lentivirus copy number - (reply: 7)
148. Genomic -Lactamase? Blue White screening not working - (reply: 1)
149. direction of the expression units in multi-gene vectors - (reply: 1)
150. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)