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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Help Quesion - (reply: 1)
122. No colonies after self Circularization ligation - (reply: 2)
123. Clean ligation controls but no insert - (reply: 14)
124. ratios insert/vector - (reply: 3)
125. Can't isolate plasmid from cloned E.coli - (reply: 4)
126. Genomic DNA quantification using the NanoDrop - (reply: 3)
127. Competent cells with Hanahan's method - (reply: 2)
128. cloning issues - (reply: 3)
129. Is important the direction of IRES-GFP in cloning? - (reply: 1)
130. What are the error-rates of the TA cloning Kits? - (reply: 8)
131. Can mutations be created during cloning? - (reply: 7)
132. confusing with where to put primers and which ordination - (reply: 4)
133. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
134. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
135. RBS - (reply: 1)
136. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
137. Gel for my ligation - (reply: 1)
138. Amplifying from pcr products - (reply: 7)
139. Design the primers - (reply: 10)
140. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
141. How two fragments joint together when doing fusion PCR? - (reply: 4)
142. Working backwards from a construct? - (reply: 1)
143. disappearing bases - (reply: 4)
144. Mutagenesis PCR - (reply: 9)
145. Digest two sites located next to each other - (reply: 5)
146. cDNA Library Construction by using kits and Sequencing - (reply: 1)
147. Looking for software that permutes codons to find new restriction sites - (reply: 4)
148. Transforming dna from agarose gel - (reply: 3)
149. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
150. ccdb toxin - (reply: 15)