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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Cloning beginner and clueless! - (reply: 1)
122. Which epitope tag to use for ChIP? - (reply: 1)
123. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
124. Ligation-transformation...Never faced such problem ever - (reply: 6)
125. Can multiple genes follow only one promoter? - (reply: 1)
126. Restriction Digest Question - (reply: 4)
127. Problem with Large vector & very small insert - (reply: 1)
128. Storage life of plasmid DNA in water - (reply: 5)
129. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
130. Site-directed mutagenesis failed - (reply: 20)
131. Insert & vector too dilute to perform ligation - (reply: 3)
132. Cloning Problems - (reply: 2)
133. Suitable templates for in vitro transcription - (reply: 1)
134. Different ways to ensure the successful cloning? - (reply: 2)
135. Plasmid DNA digestion problem - (reply: 3)
136. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
137. Single codon insert - (reply: 3)
138. cloning- ligation - (reply: 3)
139. Sticky and blunt ligation issue - (reply: 6)
140. Handling DEPC under nitrogen/argon - (reply: 3)
141. Incubation period after heat shock at transformation--reason? - (reply: 3)
142. PCR product sequencing - (reply: 3)
143. What is the optimal time for transfection efficiency measuring? - (reply: 1)
144. i couldn't get the band of target gene after ligation - (reply: 9)
145. Minimum homology bases needed for homologous recombination - (reply: 1)
146. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
147. Expansion of commercial competent bacterial cell lines? - (reply: 2)
148. Manipulation of target DNA fragments - (reply: 1)
149. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
150. Appearance of a salty plasmid preparation - (reply: 1)