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Top : New Forum Archives (2009-): : Molecular-Cloning
121. All I get is supercoiled DNA... - (reply: 5)
122. Help Quesion - (reply: 1)
123. No colonies after self Circularization ligation - (reply: 2)
124. Clean ligation controls but no insert - (reply: 14)
125. ratios insert/vector - (reply: 3)
126. Can't isolate plasmid from cloned E.coli - (reply: 4)
127. Genomic DNA quantification using the NanoDrop - (reply: 3)
128. Competent cells with Hanahan's method - (reply: 2)
129. cloning issues - (reply: 3)
130. Is important the direction of IRES-GFP in cloning? - (reply: 1)
131. What are the error-rates of the TA cloning Kits? - (reply: 8)
132. Can mutations be created during cloning? - (reply: 7)
133. confusing with where to put primers and which ordination - (reply: 4)
134. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
135. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
136. RBS - (reply: 1)
137. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
138. Gel for my ligation - (reply: 1)
139. Amplifying from pcr products - (reply: 7)
140. Design the primers - (reply: 10)
141. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
142. How two fragments joint together when doing fusion PCR? - (reply: 4)
143. Working backwards from a construct? - (reply: 1)
144. disappearing bases - (reply: 4)
145. Mutagenesis PCR - (reply: 9)
146. Digest two sites located next to each other - (reply: 5)
147. cDNA Library Construction by using kits and Sequencing - (reply: 1)
148. Looking for software that permutes codons to find new restriction sites - (reply: 4)
149. Transforming dna from agarose gel - (reply: 3)
150. Cloning GFP into pCMV-Tag2B vector - (reply: 1)