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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Design the primers - (reply: 10)
122. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
123. How two fragments joint together when doing fusion PCR? - (reply: 4)
124. Working backwards from a construct? - (reply: 1)
125. disappearing bases - (reply: 4)
126. Mutagenesis PCR - (reply: 9)
127. Digest two sites located next to each other - (reply: 5)
128. cDNA Library Construction by using kits and Sequencing - (reply: 1)
129. Looking for software that permutes codons to find new restriction sites - (reply: 4)
130. Transforming dna from agarose gel - (reply: 3)
131. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
132. ccdb toxin - (reply: 15)
133. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
134. annealing oligos - (reply: 3)
135. Does a restriction enzyme cut at a related site? - (reply: 5)
136. primers for cloning - (reply: 1)
137. Vector-insert ratio - (reply: 6)
138. Cloning contamination - (reply: 3)
139. plasmid construction - (reply: 2)
140. primer design with restriction enzyme - (reply: 1)
141. vector propagation in E coli - (reply: 1)
142. Restriction to remove background - (reply: 4)
143. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
144. Restriction and ligation time interval - (reply: 1)
145. Problem shrna cloning, bad sequencing results - (reply: 1)
146. Lentivirus copy number - (reply: 7)
147. Genomic -Lactamase? Blue White screening not working - (reply: 1)
148. direction of the expression units in multi-gene vectors - (reply: 1)
149. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
150. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)