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Top : New Forum Archives (2009-): : Molecular-Cloning
121. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
122. Deleting 7Mbp chromosomal region - (reply: 3)
123. Electrophoresis samples and volumes - (reply: 1)
124. Gene cloning with unknown sequence - (reply: 6)
125. Checking for efficieny competent cells - (reply: 1)
126. Troubles with Fusion PCR - (reply: 1)
127. Does this look alright? - (reply: 3)
128. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
129. Cloning pDsRed-Monomer-N1 - (reply: 1)
130. forgot to heat shock - (reply: 6)
131. Ligation left for weekend at room temp - (reply: 4)
132. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
133. Problem in plasmid digestion after transformation - (reply: 4)
134. Lac Promoter activity in P. aeruginosa - (reply: 1)
135. Resctriction analysis - (reply: 5)
136. Please comment on my Cloning Plan - (reply: 1)
137. Gibson/SLIC question - (reply: 1)
138. How to determine where, in the genome, my transgene inserted - (reply: 4)
139. no colonies, ever! - (reply: 5)
140. fluorescent tag on membrane protein - (reply: 2)
141. How can addgene sell plasmids with patented technology - (reply: 2)
142. no insert issue - (reply: 9)
143. Considerations on the design of a transgene - (reply: 1)
144. How to obtain Vectors used in articles - (reply: 5)
145. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
146. How efficient is the 2A sequence - (reply: 2)
147. Transformation keeps failing - (reply: 2)
148. High background issue - (reply: 5)
149. Pcr primers - (reply: 7)
150. Why loading buffer can't go down after enzyme digestion? - (reply: 1)