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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Looking for software that permutes codons to find new restriction sites - (reply: 4)
122. Transforming dna from agarose gel - (reply: 3)
123. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
124. ccdb toxin - (reply: 15)
125. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
126. annealing oligos - (reply: 3)
127. Does a restriction enzyme cut at a related site? - (reply: 5)
128. primers for cloning - (reply: 1)
129. Vector-insert ratio - (reply: 6)
130. Cloning contamination - (reply: 3)
131. plasmid construction - (reply: 2)
132. primer design with restriction enzyme - (reply: 1)
133. vector propagation in E coli - (reply: 1)
134. Restriction to remove background - (reply: 4)
135. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
136. Restriction and ligation time interval - (reply: 1)
137. Problem shrna cloning, bad sequencing results - (reply: 1)
138. Lentivirus copy number - (reply: 7)
139. Genomic -Lactamase? Blue White screening not working - (reply: 1)
140. direction of the expression units in multi-gene vectors - (reply: 1)
141. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
142. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
143. Measuring pcr fragments in a gel - (reply: 1)
144. gel electrophoresis - (reply: 4)
145. three questions about CRISPR knowout - (reply: 2)
146. Help needed for Ligation/Transformation - (reply: 1)
147. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
148. ClaI restriction enzyme efficency - (reply: 1)
149. Blunt end cloning - (reply: 12)
150. sequantial digest - (reply: 25)