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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Subtle change in insert size after cloning - (reply: 6)
122. lactic acid bacteria - cloning - (reply: 2)
123. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
124. All I get is supercoiled DNA... - (reply: 5)
125. Help Quesion - (reply: 1)
126. No colonies after self Circularization ligation - (reply: 2)
127. Clean ligation controls but no insert - (reply: 14)
128. ratios insert/vector - (reply: 3)
129. Can't isolate plasmid from cloned E.coli - (reply: 4)
130. Genomic DNA quantification using the NanoDrop - (reply: 3)
131. Competent cells with Hanahan's method - (reply: 2)
132. cloning issues - (reply: 3)
133. Is important the direction of IRES-GFP in cloning? - (reply: 1)
134. What are the error-rates of the TA cloning Kits? - (reply: 8)
135. Can mutations be created during cloning? - (reply: 7)
136. confusing with where to put primers and which ordination - (reply: 4)
137. Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
138. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
139. RBS - (reply: 1)
140. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
141. Gel for my ligation - (reply: 1)
142. Amplifying from pcr products - (reply: 7)
143. Design the primers - (reply: 10)
144. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
145. How two fragments joint together when doing fusion PCR? - (reply: 4)
146. Working backwards from a construct? - (reply: 1)
147. disappearing bases - (reply: 4)
148. Mutagenesis PCR - (reply: 9)
149. Digest two sites located next to each other - (reply: 5)
150. cDNA Library Construction by using kits and Sequencing - (reply: 1)