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Top : New Forum Archives (2009-): : Molecular-Cloning
121. annealing oligos - (reply: 3)
122. Does a restriction enzyme cut at a related site? - (reply: 5)
123. primers for cloning - (reply: 1)
124. Vector-insert ratio - (reply: 6)
125. Cloning contamination - (reply: 3)
126. plasmid construction - (reply: 2)
127. primer design with restriction enzyme - (reply: 1)
128. vector propagation in E coli - (reply: 1)
129. Restriction to remove background - (reply: 4)
130. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
131. Restriction and ligation time interval - (reply: 1)
132. Problem shrna cloning, bad sequencing results - (reply: 1)
133. Lentivirus copy number - (reply: 7)
134. Genomic -Lactamase? Blue White screening not working - (reply: 1)
135. direction of the expression units in multi-gene vectors - (reply: 1)
136. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
137. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
138. Measuring pcr fragments in a gel - (reply: 1)
139. gel electrophoresis - (reply: 4)
140. three questions about CRISPR knowout - (reply: 2)
141. Help needed for Ligation/Transformation - (reply: 1)
142. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
143. ClaI restriction enzyme efficency - (reply: 1)
144. Blunt end cloning - (reply: 12)
145. sequantial digest - (reply: 25)
146. Temperature sensitivity of Plasmids - (reply: 1)
147. DMSO in restriction digest - (reply: 1)
148. Molecular cloning - (reply: 5)
149. Nuclease free water for cloning - (reply: 8)
150. Directional cloning 2bps overhang - (reply: 3)