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Top : New Forum Archives (2009-): : Molecular-Cloning
121. Gel for my ligation - (reply: 1)
122. Amplifying from pcr products - (reply: 7)
123. Design the primers - (reply: 10)
124. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
125. How two fragments joint together when doing fusion PCR? - (reply: 4)
126. Working backwards from a construct? - (reply: 1)
127. disappearing bases - (reply: 4)
128. Mutagenesis PCR - (reply: 9)
129. Digest two sites located next to each other - (reply: 5)
130. cDNA Library Construction by using kits and Sequencing - (reply: 1)
131. Looking for software that permutes codons to find new restriction sites - (reply: 4)
132. Transforming dna from agarose gel - (reply: 3)
133. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
134. ccdb toxin - (reply: 15)
135. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
136. annealing oligos - (reply: 3)
137. Does a restriction enzyme cut at a related site? - (reply: 5)
138. primers for cloning - (reply: 1)
139. Vector-insert ratio - (reply: 6)
140. Cloning contamination - (reply: 3)
141. plasmid construction - (reply: 2)
142. primer design with restriction enzyme - (reply: 1)
143. vector propagation in E coli - (reply: 1)
144. Restriction to remove background - (reply: 4)
145. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
146. Restriction and ligation time interval - (reply: 1)
147. Problem shrna cloning, bad sequencing results - (reply: 1)
148. Lentivirus copy number - (reply: 7)
149. Genomic -Lactamase? Blue White screening not working - (reply: 1)
150. direction of the expression units in multi-gene vectors - (reply: 1)