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Top : New Forum Archives (2009-): : Molecular Cloning

1651. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1652. Gel purification of vector DNA - low yield - (reply: 26)
1653. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1654. Identifying cDNA insert - Basic help needed (reply: 2)
1655. gel purification or ligation problem - (reply: 3)
1656. Sequential restriction digestion of plasmid - (reply: 58)
1657. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1658. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1659. Transformation question - (reply: 9)
1660. Cloning trouble - (reply: 5)
1661. Problem cloning oligo into double digested vector - (reply: 9)
1662. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1663. endonuclease contamination in mini-preps - (reply: 8)
1664. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
1665. cloning 1052bp fragment - no colonies (reply: 11)
1666. What is the biggest insert for a viral vector? - (reply: 15)
1667. Stored bacteria lost plasmids? - (reply: 12)
1668. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
1669. RNA extraction in -80 - (reply: 1)
1670. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
1671. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
1672. making competent E coli DH5a cells - (reply: 6)
1673. Ligation very large vector and small insert - It will not work... (reply: 7)
1674. kpi ? - (reply: 1)
1675. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
1676. Restriction enzyme digest - (reply: 19)
1677. Long PCR product - (reply: 9)
1678. Elute cDNA from filter paper - (reply: 1)
1679. cDNA to find - (reply: 1)