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Top : New Forum Archives (2009-): : Molecular-Cloning
1651. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1652. yeast transformation - how to make a thermotolerant yeast (reply: 4)
1653. need help with klenow - (reply: 4)
1654. Subcloning into pBluescript - (reply: 6)
1655. How to control cloned cDNA expression level in cells? - (reply: 8)
1656. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1657. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1658. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1659. Gel purification of vector DNA - low yield - (reply: 26)
1660. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1661. Identifying cDNA insert - Basic help needed (reply: 2)
1662. gel purification or ligation problem - (reply: 3)
1663. Sequential restriction digestion of plasmid - (reply: 58)
1664. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1665. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1666. Transformation question - (reply: 9)
1667. Cloning trouble - (reply: 5)
1668. Problem cloning oligo into double digested vector - (reply: 9)
1669. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1670. endonuclease contamination in mini-preps - (reply: 8)
1671. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
1672. cloning 1052bp fragment - no colonies (reply: 11)
1673. What is the biggest insert for a viral vector? - (reply: 15)
1674. Stored bacteria lost plasmids? - (reply: 12)
1675. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
1676. RNA extraction in -80 - (reply: 1)
1677. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
1678. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
1679. making competent E coli DH5a cells - (reply: 6)
1680. Ligation very large vector and small insert - It will not work... (reply: 7)