Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1651. Subcloning into pBluescript - (reply: 6)
1652. How to control cloned cDNA expression level in cells? - (reply: 8)
1653. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1654. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1655. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1656. Gel purification of vector DNA - low yield - (reply: 26)
1657. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1658. Identifying cDNA insert - Basic help needed (reply: 2)
1659. gel purification or ligation problem - (reply: 3)
1660. Sequential restriction digestion of plasmid - (reply: 58)
1661. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1662. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1663. Transformation question - (reply: 9)
1664. Cloning trouble - (reply: 5)
1665. Problem cloning oligo into double digested vector - (reply: 9)
1666. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1667. endonuclease contamination in mini-preps - (reply: 8)
1668. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
1669. cloning 1052bp fragment - no colonies (reply: 11)
1670. What is the biggest insert for a viral vector? - (reply: 15)
1671. Stored bacteria lost plasmids? - (reply: 12)
1672. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
1673. RNA extraction in -80 - (reply: 1)
1674. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
1675. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
1676. making competent E coli DH5a cells - (reply: 6)
1677. Ligation very large vector and small insert - It will not work... (reply: 7)
1678. kpi ? - (reply: 1)
1679. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
1680. Restriction enzyme digest - (reply: 19)