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Top : New Forum Archives (2009-): : Molecular Cloning

1651. Gel purification of vector DNA - low yield - (reply: 26)
1652. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1653. Identifying cDNA insert - Basic help needed (reply: 2)
1654. gel purification or ligation problem - (reply: 3)
1655. Sequential restriction digestion of plasmid - (reply: 58)
1656. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1657. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1658. Transformation question - (reply: 9)
1659. Cloning trouble - (reply: 5)
1660. Problem cloning oligo into double digested vector - (reply: 9)
1661. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1662. endonuclease contamination in mini-preps - (reply: 8)
1663. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)
1664. cloning 1052bp fragment - no colonies (reply: 11)
1665. What is the biggest insert for a viral vector? - (reply: 15)
1666. Stored bacteria lost plasmids? - (reply: 12)
1667. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
1668. RNA extraction in -80 - (reply: 1)
1669. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
1670. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
1671. making competent E coli DH5a cells - (reply: 6)
1672. Ligation very large vector and small insert - It will not work... (reply: 7)
1673. kpi ? - (reply: 1)
1674. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)
1675. Restriction enzyme digest - (reply: 19)
1676. Long PCR product - (reply: 9)
1677. Elute cDNA from filter paper - (reply: 1)
1678. cDNA to find - (reply: 1)