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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Cloning does not work - (reply: 4)
872. Small Colonies After Transformation - (reply: 5)
873. Ligation and transformation problem - (reply: 2)
874. Agrobacterium EHA101 competent cells - (reply: 1)
875. restriction digestion against colony PCR - (reply: 1)
876. BAC for transgenic mice - (reply: 6)
877. Tag proteins for CO-IP and confocal - (reply: 1)
878. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
879. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
880. how long for a double digestion - (reply: 2)
881. Problems with Site-Directed Mutagenesis - (reply: 2)
882. blunt and sticky end clonning temperature - (reply: 2)
883. Most common mistakes - What mistakes are made during cloning? (reply: 7)
884. Luciferase Assay Stim. Concentration? - (reply: 1)
885. Cloning direction / orientation? - (reply: 2)
886. Question about plasmid midiprep - (reply: 1)
887. Does a polyA sequence need to be put in-frame? - (reply: 1)
888. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
889. Problem on growing up bugs - (reply: 8)
890. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
891. pSIM in BL21(DE3) - (reply: 2)
892. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
893. Weird problem in invitromutagenesis - (reply: 3)
894. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
895. RE digestion - (reply: 4)
896. HELP!!! - (reply: 3)
897. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
898. Cloning of ITS region - (reply: 6)
899. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
900. knockdown: yes; GFP: no ?! what is this? - (reply: 2)