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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Small Colonies After Transformation - (reply: 5)
872. Ligation and transformation problem - (reply: 2)
873. Agrobacterium EHA101 competent cells - (reply: 1)
874. restriction digestion against colony PCR - (reply: 1)
875. BAC for transgenic mice - (reply: 6)
876. Tag proteins for CO-IP and confocal - (reply: 1)
877. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
878. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
879. how long for a double digestion - (reply: 2)
880. Problems with Site-Directed Mutagenesis - (reply: 2)
881. blunt and sticky end clonning temperature - (reply: 2)
882. Most common mistakes - What mistakes are made during cloning? (reply: 7)
883. Luciferase Assay Stim. Concentration? - (reply: 1)
884. Cloning direction / orientation? - (reply: 2)
885. Question about plasmid midiprep - (reply: 1)
886. Does a polyA sequence need to be put in-frame? - (reply: 1)
887. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
888. Problem on growing up bugs - (reply: 8)
889. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
890. pSIM in BL21(DE3) - (reply: 2)
891. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
892. Weird problem in invitromutagenesis - (reply: 3)
893. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
894. RE digestion - (reply: 4)
895. HELP!!! - (reply: 3)
896. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
897. Cloning of ITS region - (reply: 6)
898. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
899. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
900. SmaI blunt end ligation problem... - (reply: 3)