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Top : New Forum Archives (2009-): : Molecular-Cloning
871. 126pb insert clonnig/ digestion issues - (reply: 3)
872. cloning problem... - (reply: 5)
873. yeast glycerol stock - (reply: 2)
874. yeast transformation - (reply: 1)
875. Problems with ligation and transformation - (reply: 2)
876. very small insert and a very ling vector - (reply: 1)
877. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
878. cDNA clone - (reply: 1)
879. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
880. why these guys use T7 instead of CMV? - for transfection (reply: 2)
881. Electroporation - no colonies (reply: 1)
882. enzymes inactivation - (reply: 3)
883. help: why plasmid yield dropped ever since the first culture - (reply: 2)
884. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
885. miRNA cloning - (reply: 2)
886. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
887. Single-digested vector shifts downward on gel - (reply: 1)
888. problem in digestion - (reply: 1)
889. Cloning does not work - (reply: 4)
890. Small Colonies After Transformation - (reply: 5)
891. Ligation and transformation problem - (reply: 2)
892. Agrobacterium EHA101 competent cells - (reply: 1)
893. restriction digestion against colony PCR - (reply: 1)
894. BAC for transgenic mice - (reply: 6)
895. Tag proteins for CO-IP and confocal - (reply: 1)
896. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
897. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
898. how long for a double digestion - (reply: 2)
899. Problems with Site-Directed Mutagenesis - (reply: 2)
900. blunt and sticky end clonning temperature - (reply: 2)