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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Ligation and transformation problem - (reply: 2)
872. Agrobacterium EHA101 competent cells - (reply: 1)
873. restriction digestion against colony PCR - (reply: 1)
874. BAC for transgenic mice - (reply: 6)
875. Tag proteins for CO-IP and confocal - (reply: 1)
876. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
877. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
878. how long for a double digestion - (reply: 2)
879. Problems with Site-Directed Mutagenesis - (reply: 2)
880. blunt and sticky end clonning temperature - (reply: 2)
881. Most common mistakes - What mistakes are made during cloning? (reply: 7)
882. Luciferase Assay Stim. Concentration? - (reply: 1)
883. Cloning direction / orientation? - (reply: 2)
884. Question about plasmid midiprep - (reply: 1)
885. Does a polyA sequence need to be put in-frame? - (reply: 1)
886. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
887. Problem on growing up bugs - (reply: 8)
888. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
889. pSIM in BL21(DE3) - (reply: 2)
890. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
891. Weird problem in invitromutagenesis - (reply: 3)
892. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
893. RE digestion - (reply: 4)
894. HELP!!! - (reply: 3)
895. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
896. Cloning of ITS region - (reply: 6)
897. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
898. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
899. SmaI blunt end ligation problem... - (reply: 3)
900. AAV construct? - (reply: 1)