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Top : New Forum Archives (2009-): : Molecular-Cloning
871. cDNA clone - (reply: 1)
872. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
873. why these guys use T7 instead of CMV? - for transfection (reply: 2)
874. Electroporation - no colonies (reply: 1)
875. enzymes inactivation - (reply: 3)
876. help: why plasmid yield dropped ever since the first culture - (reply: 2)
877. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
878. miRNA cloning - (reply: 2)
879. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
880. Single-digested vector shifts downward on gel - (reply: 1)
881. problem in digestion - (reply: 1)
882. Cloning does not work - (reply: 4)
883. Small Colonies After Transformation - (reply: 5)
884. Ligation and transformation problem - (reply: 2)
885. Agrobacterium EHA101 competent cells - (reply: 1)
886. restriction digestion against colony PCR - (reply: 1)
887. BAC for transgenic mice - (reply: 6)
888. Tag proteins for CO-IP and confocal - (reply: 1)
889. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
890. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
891. how long for a double digestion - (reply: 2)
892. Problems with Site-Directed Mutagenesis - (reply: 2)
893. blunt and sticky end clonning temperature - (reply: 2)
894. Most common mistakes - What mistakes are made during cloning? (reply: 7)
895. Luciferase Assay Stim. Concentration? - (reply: 1)
896. Cloning direction / orientation? - (reply: 2)
897. Question about plasmid midiprep - (reply: 1)
898. Does a polyA sequence need to be put in-frame? - (reply: 1)
899. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
900. Problem on growing up bugs - (reply: 8)