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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
872. Starting cells for making one's own competent cells - (reply: 7)
873. 6 months... and still no positive clones! - (reply: 4)
874. Really strange problem with sequencing and plasmid purification! - (reply: 8)
875. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
876. what is site-specific integration - (reply: 2)
877. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
878. double transformations - (reply: 1)
879. Unusual band in transformants - (reply: 2)
880. ligation--pls help! - (reply: 10)
881. bizarre digestion:) - (reply: 2)
882. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
883. metagenomic library consturction - BAC vectors (reply: 1)
884. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
885. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
886. Chromosomal recombination with BL21? - (reply: 2)
887. Restriction Digestion!! - (reply: 5)
888. digestion - restriction enzymes (reply: 2)
889. How to prevent self ligation - selfligation (reply: 11)
890. what is the difference between DH10B and Top10 - (reply: 1)
891. problems encountered when working on a large plasmid - (reply: 2)
892. make a Mega plasmid mutation - (reply: 1)
893. Cloning - (reply: 1)
894. Copy-number calculation formula? - (reply: 1)
895. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
896. low melt agarose - (reply: 1)
897. restriction digest - smear - (reply: 1)
898. designing of primers - (reply: 2)
899. Propagating ccdB survival cells - (reply: 6)
900. Bicistronic vector vs "normal" vector - (reply: 5)