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Top : New Forum Archives (2009-): : Molecular-Cloning
871. restriction digestion against colony PCR - (reply: 1)
872. BAC for transgenic mice - (reply: 6)
873. Tag proteins for CO-IP and confocal - (reply: 1)
874. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
875. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
876. how long for a double digestion - (reply: 2)
877. Problems with Site-Directed Mutagenesis - (reply: 2)
878. blunt and sticky end clonning temperature - (reply: 2)
879. Most common mistakes - What mistakes are made during cloning? (reply: 7)
880. Luciferase Assay Stim. Concentration? - (reply: 1)
881. Cloning direction / orientation? - (reply: 2)
882. Question about plasmid midiprep - (reply: 1)
883. Does a polyA sequence need to be put in-frame? - (reply: 1)
884. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
885. Problem on growing up bugs - (reply: 8)
886. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
887. pSIM in BL21(DE3) - (reply: 2)
888. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
889. Weird problem in invitromutagenesis - (reply: 3)
890. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
891. RE digestion - (reply: 4)
892. HELP!!! - (reply: 3)
893. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
894. Cloning of ITS region - (reply: 6)
895. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
896. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
897. SmaI blunt end ligation problem... - (reply: 3)
898. AAV construct? - (reply: 1)
899. No bands on agarose gel after restriction digestion. - (reply: 10)
900. inverse pcr - (reply: 2)