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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Problems with ligation and transformation - (reply: 2)
872. very small insert and a very ling vector - (reply: 1)
873. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
874. cDNA clone - (reply: 1)
875. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
876. why these guys use T7 instead of CMV? - for transfection (reply: 2)
877. Electroporation - no colonies (reply: 1)
878. enzymes inactivation - (reply: 3)
879. help: why plasmid yield dropped ever since the first culture - (reply: 2)
880. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
881. miRNA cloning - (reply: 2)
882. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
883. Single-digested vector shifts downward on gel - (reply: 1)
884. problem in digestion - (reply: 1)
885. Cloning does not work - (reply: 4)
886. Small Colonies After Transformation - (reply: 5)
887. Ligation and transformation problem - (reply: 2)
888. Agrobacterium EHA101 competent cells - (reply: 1)
889. restriction digestion against colony PCR - (reply: 1)
890. BAC for transgenic mice - (reply: 6)
891. Tag proteins for CO-IP and confocal - (reply: 1)
892. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
893. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
894. how long for a double digestion - (reply: 2)
895. Problems with Site-Directed Mutagenesis - (reply: 2)
896. blunt and sticky end clonning temperature - (reply: 2)
897. Most common mistakes - What mistakes are made during cloning? (reply: 7)
898. Luciferase Assay Stim. Concentration? - (reply: 1)
899. Cloning direction / orientation? - (reply: 2)
900. Question about plasmid midiprep - (reply: 1)