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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
872. Break Cell Clumps - prepare single cells for injections (reply: 1)
873. 126pb insert clonnig/ digestion issues - (reply: 3)
874. cloning problem... - (reply: 5)
875. yeast glycerol stock - (reply: 2)
876. yeast transformation - (reply: 1)
877. Problems with ligation and transformation - (reply: 2)
878. very small insert and a very ling vector - (reply: 1)
879. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
880. cDNA clone - (reply: 1)
881. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
882. why these guys use T7 instead of CMV? - for transfection (reply: 2)
883. Electroporation - no colonies (reply: 1)
884. enzymes inactivation - (reply: 3)
885. help: why plasmid yield dropped ever since the first culture - (reply: 2)
886. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
887. miRNA cloning - (reply: 2)
888. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
889. Single-digested vector shifts downward on gel - (reply: 1)
890. problem in digestion - (reply: 1)
891. Cloning does not work - (reply: 4)
892. Small Colonies After Transformation - (reply: 5)
893. Ligation and transformation problem - (reply: 2)
894. Agrobacterium EHA101 competent cells - (reply: 1)
895. restriction digestion against colony PCR - (reply: 1)
896. BAC for transgenic mice - (reply: 6)
897. Tag proteins for CO-IP and confocal - (reply: 1)
898. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
899. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
900. how long for a double digestion - (reply: 2)