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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Problems with Site-Directed Mutagenesis - (reply: 2)
872. blunt and sticky end clonning temperature - (reply: 2)
873. Most common mistakes - What mistakes are made during cloning? (reply: 7)
874. Luciferase Assay Stim. Concentration? - (reply: 1)
875. Cloning direction / orientation? - (reply: 2)
876. Question about plasmid midiprep - (reply: 1)
877. Does a polyA sequence need to be put in-frame? - (reply: 1)
878. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
879. Problem on growing up bugs - (reply: 8)
880. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
881. pSIM in BL21(DE3) - (reply: 2)
882. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
883. Weird problem in invitromutagenesis - (reply: 3)
884. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
885. RE digestion - (reply: 4)
886. HELP!!! - (reply: 3)
887. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
888. Cloning of ITS region - (reply: 6)
889. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
890. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
891. SmaI blunt end ligation problem... - (reply: 3)
892. AAV construct? - (reply: 1)
893. No bands on agarose gel after restriction digestion. - (reply: 10)
894. inverse pcr - (reply: 2)
895. Viral cDNA Library - (reply: 3)
896. metylated cloning vector - restriction digest of the insert (reply: 2)
897. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
898. invitro mutagenesis using stragene kit - (reply: 4)
899. Secretion cloning vectors - (reply: 1)
900. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)