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Top : New Forum Archives (2009-): : Molecular-Cloning
871. complementing a double knockout in E. coli - (reply: 1)
872. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
873. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
874. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
875. which G418 concentration and treatment period should be used? - (reply: 2)
876. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
877. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
878. Chemical competent E.coli always from single colony ? - (reply: 2)
879. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
880. Strange results of cloning - insert always reverse-oriented (reply: 2)
881. electroporation - (reply: 2)
882. transfection or infection - difference? (reply: 1)
883. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
884. No colonies after transformation! - (reply: 2)
885. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
886. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
887. Help Miniprep kit SIGMA - (reply: 2)
888. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
889. A question about pBR322 and pUC ori - (reply: 3)
890. Digestion of mini-prep by mistake - (reply: 3)
891. Cloning issue - (reply: 3)
892. Cohesive end filling after CIAP treatment - (reply: 2)
893. Looking for Pfu Taq for high GC-content template - (reply: 7)
894. questions about transformation - (reply: 4)
895. genotype lysogen DE3 - (reply: 1)
896. Need help! - Problem with right insertion! (reply: 1)
897. cloning a large insert - (reply: 5)
898. Transposase contamination in cloning - (reply: 7)
899. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
900. How long can I store cleaved/digested vector? - (reply: 9)