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Top : New Forum Archives (2009-): : Molecular-Cloning
871. yeast glycerol stock - (reply: 2)
872. yeast transformation - (reply: 1)
873. Problems with ligation and transformation - (reply: 2)
874. very small insert and a very ling vector - (reply: 1)
875. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
876. cDNA clone - (reply: 1)
877. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
878. why these guys use T7 instead of CMV? - for transfection (reply: 2)
879. Electroporation - no colonies (reply: 1)
880. enzymes inactivation - (reply: 3)
881. help: why plasmid yield dropped ever since the first culture - (reply: 2)
882. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
883. miRNA cloning - (reply: 2)
884. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
885. Single-digested vector shifts downward on gel - (reply: 1)
886. problem in digestion - (reply: 1)
887. Cloning does not work - (reply: 4)
888. Small Colonies After Transformation - (reply: 5)
889. Ligation and transformation problem - (reply: 2)
890. Agrobacterium EHA101 competent cells - (reply: 1)
891. restriction digestion against colony PCR - (reply: 1)
892. BAC for transgenic mice - (reply: 6)
893. Tag proteins for CO-IP and confocal - (reply: 1)
894. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
895. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
896. how long for a double digestion - (reply: 2)
897. Problems with Site-Directed Mutagenesis - (reply: 2)
898. blunt and sticky end clonning temperature - (reply: 2)
899. Most common mistakes - What mistakes are made during cloning? (reply: 7)
900. Luciferase Assay Stim. Concentration? - (reply: 1)