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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Problem on growing up bugs - (reply: 8)
872. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
873. pSIM in BL21(DE3) - (reply: 2)
874. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
875. Weird problem in invitromutagenesis - (reply: 3)
876. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
877. RE digestion - (reply: 4)
878. HELP!!! - (reply: 3)
879. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
880. Cloning of ITS region - (reply: 6)
881. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
882. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
883. SmaI blunt end ligation problem... - (reply: 3)
884. AAV construct? - (reply: 1)
885. No bands on agarose gel after restriction digestion. - (reply: 10)
886. inverse pcr - (reply: 2)
887. Viral cDNA Library - (reply: 3)
888. metylated cloning vector - restriction digest of the insert (reply: 2)
889. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
890. invitro mutagenesis using stragene kit - (reply: 4)
891. Secretion cloning vectors - (reply: 1)
892. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
893. complementing a double knockout in E. coli - (reply: 1)
894. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
895. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
896. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
897. which G418 concentration and treatment period should be used? - (reply: 2)
898. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
899. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
900. Chemical competent E.coli always from single colony ? - (reply: 2)