Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
871. how long for a double digestion - (reply: 2)
872. Problems with Site-Directed Mutagenesis - (reply: 2)
873. blunt and sticky end clonning temperature - (reply: 2)
874. Most common mistakes - What mistakes are made during cloning? (reply: 7)
875. Luciferase Assay Stim. Concentration? - (reply: 1)
876. Cloning direction / orientation? - (reply: 2)
877. Question about plasmid midiprep - (reply: 1)
878. Does a polyA sequence need to be put in-frame? - (reply: 1)
879. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
880. Problem on growing up bugs - (reply: 8)
881. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
882. pSIM in BL21(DE3) - (reply: 2)
883. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
884. Weird problem in invitromutagenesis - (reply: 3)
885. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
886. RE digestion - (reply: 4)
887. HELP!!! - (reply: 3)
888. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
889. Cloning of ITS region - (reply: 6)
890. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
891. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
892. SmaI blunt end ligation problem... - (reply: 3)
893. AAV construct? - (reply: 1)
894. No bands on agarose gel after restriction digestion. - (reply: 10)
895. inverse pcr - (reply: 2)
896. Viral cDNA Library - (reply: 3)
897. metylated cloning vector - restriction digest of the insert (reply: 2)
898. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
899. invitro mutagenesis using stragene kit - (reply: 4)
900. Secretion cloning vectors - (reply: 1)