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871. blue white screening - (reply: 3)
872. phosphorylate dprimers for pfx do not work - (reply: 3)
873. help - (reply: 1)
874. problem with yeast transformation - (reply: 2)
875. ta cloning of pfu amplified fragment - (reply: 9)
876. how to express the gene in opposite transcriptional direction - (reply: 3)
877. Water for electrocompetent cells - (reply: 2)
878. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
879. Bacterial growth in agar plate with ampicillin - (reply: 4)
880. problem in ligation and cloning - (reply: 1)
881. Electrophoresis gel boxes and geldoc system - (reply: 2)
882. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
883. problem with 293T cell co-transfection - (reply: 5)
884. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
885. how CIP treatment is done - (reply: 3)
886. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
887. Storage of gel extracted DNA before ligation - (reply: 3)
888. Dual Luciferase Assay - (reply: 3)
889. Ligation Problems - (reply: 2)
890. NICKED DNA - (reply: 2)
891. cDNA clone - (reply: 1)
892. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
893. Chosing the right competent cells - (reply: 1)
894. Checking PCR insert (into pGEMT vector) - (reply: 2)
895. problem with pGEMT - (reply: 3)
896. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
897. Luciferase GL4 siRNA - (reply: 5)
898. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
899. Culture Grow well but No Plasmid - (reply: 2)
900. Insertion Mutation in Insert Sequence - (reply: 2)
872. phosphorylate dprimers for pfx do not work - (reply: 3)
873. help - (reply: 1)
874. problem with yeast transformation - (reply: 2)
875. ta cloning of pfu amplified fragment - (reply: 9)
876. how to express the gene in opposite transcriptional direction - (reply: 3)
877. Water for electrocompetent cells - (reply: 2)
878. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
879. Bacterial growth in agar plate with ampicillin - (reply: 4)
880. problem in ligation and cloning - (reply: 1)
881. Electrophoresis gel boxes and geldoc system - (reply: 2)
882. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)
883. problem with 293T cell co-transfection - (reply: 5)
884. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
885. how CIP treatment is done - (reply: 3)
886. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
887. Storage of gel extracted DNA before ligation - (reply: 3)
888. Dual Luciferase Assay - (reply: 3)
889. Ligation Problems - (reply: 2)
890. NICKED DNA - (reply: 2)
891. cDNA clone - (reply: 1)
892. Insertion of fragment with same 5' and 3' ends - Just out of interest (reply: 5)
893. Chosing the right competent cells - (reply: 1)
894. Checking PCR insert (into pGEMT vector) - (reply: 2)
895. problem with pGEMT - (reply: 3)
896. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
897. Luciferase GL4 siRNA - (reply: 5)
898. Small fragment (90bp) into an AAV backbone (4.7kb) ligation problem - I can't get the ligation (directional) to work (reply: 1)
899. Culture Grow well but No Plasmid - (reply: 2)
900. Insertion Mutation in Insert Sequence - (reply: 2)