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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
872. integration of a gene per homologous recombination - (reply: 5)
873. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
874. Antarctic Phosphatase treatment - (reply: 5)
875. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
876. phage DNA - DNA wont migrate on agarose gel (reply: 3)
877. Plasmid out of BL21 for mutagensis - (reply: 3)
878. no colony growth - (reply: 4)
879. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
880. Effect of a DNA prep on DE3 - (reply: 2)
881. Competent cell storage for long term use - (reply: 2)
882. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
883. New to sequencing, primer design - (reply: 2)
884. ta cloning problem - (reply: 2)
885. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
886. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
887. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
888. Storage of Bacterial Culture at 4 degrees - (reply: 2)
889. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
890. rna isolation - (reply: 1)
891. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
892. Molecular Cloning od Recombinant DNA - (reply: 3)
893. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
894. looking for binding site - software? (reply: 1)
895. Insertion of loxp/avoiding SINE - (reply: 1)
896. Question: Ligation Problem.... - (reply: 2)
897. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
898. Topo directional cloning - pENTR/D-TOPO (reply: 1)
899. trouble in TOPO TA sequencing - (reply: 1)
900. Large DNA extraction from an agarose gel - (reply: 8)