Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
871. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
872. RE digestion - (reply: 4)
873. HELP!!! - (reply: 3)
874. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
875. Cloning of ITS region - (reply: 6)
876. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
877. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
878. SmaI blunt end ligation problem... - (reply: 3)
879. AAV construct? - (reply: 1)
880. No bands on agarose gel after restriction digestion. - (reply: 10)
881. inverse pcr - (reply: 2)
882. Viral cDNA Library - (reply: 3)
883. metylated cloning vector - restriction digest of the insert (reply: 2)
884. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
885. invitro mutagenesis using stragene kit - (reply: 4)
886. Secretion cloning vectors - (reply: 1)
887. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
888. complementing a double knockout in E. coli - (reply: 1)
889. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
890. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
891. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
892. which G418 concentration and treatment period should be used? - (reply: 2)
893. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
894. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
895. Chemical competent E.coli always from single colony ? - (reply: 2)
896. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
897. Strange results of cloning - insert always reverse-oriented (reply: 2)
898. electroporation - (reply: 2)
899. transfection or infection - difference? (reply: 1)
900. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)