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Top : New Forum Archives (2009-): : Molecular-Cloning
871. digestion - restriction enzymes (reply: 2)
872. How to prevent self ligation - selfligation (reply: 11)
873. what is the difference between DH10B and Top10 - (reply: 1)
874. problems encountered when working on a large plasmid - (reply: 2)
875. make a Mega plasmid mutation - (reply: 1)
876. Cloning - (reply: 1)
877. Copy-number calculation formula? - (reply: 1)
878. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
879. low melt agarose - (reply: 1)
880. restriction digest - smear - (reply: 1)
881. designing of primers - (reply: 2)
882. Propagating ccdB survival cells - (reply: 6)
883. Bicistronic vector vs "normal" vector - (reply: 5)
884. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
885. Can't seem to get insert into vector... please help - (reply: 4)
886. plasmid cloning - i need help.. can anyone answer me (reply: 5)
887. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
888. Topo cloning or pGEM-T easy ? - (reply: 3)
889. Problems with qiaquick gel purification kit - cloning (reply: 10)
890. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
891. integration of a gene per homologous recombination - (reply: 5)
892. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
893. Antarctic Phosphatase treatment - (reply: 5)
894. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
895. phage DNA - DNA wont migrate on agarose gel (reply: 3)
896. Plasmid out of BL21 for mutagensis - (reply: 3)
897. no colony growth - (reply: 4)
898. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
899. Effect of a DNA prep on DE3 - (reply: 2)
900. Competent cell storage for long term use - (reply: 2)