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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
872. Help Miniprep kit SIGMA - (reply: 2)
873. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
874. A question about pBR322 and pUC ori - (reply: 3)
875. Digestion of mini-prep by mistake - (reply: 3)
876. Cloning issue - (reply: 3)
877. Cohesive end filling after CIAP treatment - (reply: 2)
878. Looking for Pfu Taq for high GC-content template - (reply: 7)
879. questions about transformation - (reply: 4)
880. genotype lysogen DE3 - (reply: 1)
881. Need help! - Problem with right insertion! (reply: 1)
882. cloning a large insert - (reply: 5)
883. Transposase contamination in cloning - (reply: 7)
884. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
885. How long can I store cleaved/digested vector? - (reply: 9)
886. Cloning of a gene containing 31 exon - (reply: 1)
887. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
888. Optimum Molar ratio for 3-way ligation - (reply: 5)
889. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
890. cloning help - (reply: 3)
891. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
892. A gene is not inserted into the Plasmid - (reply: 2)
893. Air bubbles during electroporation - (reply: 1)
894. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
895. Colonies without inserts - (reply: 9)
896. Transformants Don't Grow - (reply: 4)
897. control DNA transformation- using pGEM kit - transformation (reply: 4)
898. Topo Cloning - Understanding the principle (reply: 6)
899. pMX-PURO Vector not growing - (reply: 3)
900. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)