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Top : New Forum Archives (2009-): : Molecular-Cloning
871. transfection or infection - difference? (reply: 1)
872. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
873. No colonies after transformation! - (reply: 2)
874. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
875. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
876. Help Miniprep kit SIGMA - (reply: 2)
877. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
878. A question about pBR322 and pUC ori - (reply: 3)
879. Digestion of mini-prep by mistake - (reply: 3)
880. Cloning issue - (reply: 3)
881. Cohesive end filling after CIAP treatment - (reply: 2)
882. Looking for Pfu Taq for high GC-content template - (reply: 7)
883. questions about transformation - (reply: 4)
884. genotype lysogen DE3 - (reply: 1)
885. Need help! - Problem with right insertion! (reply: 1)
886. cloning a large insert - (reply: 5)
887. Transposase contamination in cloning - (reply: 7)
888. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
889. How long can I store cleaved/digested vector? - (reply: 9)
890. Cloning of a gene containing 31 exon - (reply: 1)
891. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
892. Optimum Molar ratio for 3-way ligation - (reply: 5)
893. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
894. cloning help - (reply: 3)
895. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
896. A gene is not inserted into the Plasmid - (reply: 2)
897. Air bubbles during electroporation - (reply: 1)
898. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
899. Colonies without inserts - (reply: 9)
900. Transformants Don't Grow - (reply: 4)