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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Single-digested vector shifts downward on gel - (reply: 1)
872. problem in digestion - (reply: 1)
873. Cloning does not work - (reply: 4)
874. Small Colonies After Transformation - (reply: 5)
875. Ligation and transformation problem - (reply: 2)
876. Agrobacterium EHA101 competent cells - (reply: 1)
877. restriction digestion against colony PCR - (reply: 1)
878. BAC for transgenic mice - (reply: 6)
879. Tag proteins for CO-IP and confocal - (reply: 1)
880. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
881. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
882. how long for a double digestion - (reply: 2)
883. Problems with Site-Directed Mutagenesis - (reply: 2)
884. blunt and sticky end clonning temperature - (reply: 2)
885. Most common mistakes - What mistakes are made during cloning? (reply: 7)
886. Luciferase Assay Stim. Concentration? - (reply: 1)
887. Cloning direction / orientation? - (reply: 2)
888. Question about plasmid midiprep - (reply: 1)
889. Does a polyA sequence need to be put in-frame? - (reply: 1)
890. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
891. Problem on growing up bugs - (reply: 8)
892. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
893. pSIM in BL21(DE3) - (reply: 2)
894. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
895. Weird problem in invitromutagenesis - (reply: 3)
896. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
897. RE digestion - (reply: 4)
898. HELP!!! - (reply: 3)
899. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
900. Cloning of ITS region - (reply: 6)