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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Break Cell Clumps - prepare single cells for injections (reply: 1)
872. 126pb insert clonnig/ digestion issues - (reply: 3)
873. cloning problem... - (reply: 5)
874. yeast glycerol stock - (reply: 2)
875. yeast transformation - (reply: 1)
876. Problems with ligation and transformation - (reply: 2)
877. very small insert and a very ling vector - (reply: 1)
878. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
879. cDNA clone - (reply: 1)
880. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
881. why these guys use T7 instead of CMV? - for transfection (reply: 2)
882. Electroporation - no colonies (reply: 1)
883. enzymes inactivation - (reply: 3)
884. help: why plasmid yield dropped ever since the first culture - (reply: 2)
885. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
886. miRNA cloning - (reply: 2)
887. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
888. Single-digested vector shifts downward on gel - (reply: 1)
889. problem in digestion - (reply: 1)
890. Cloning does not work - (reply: 4)
891. Small Colonies After Transformation - (reply: 5)
892. Ligation and transformation problem - (reply: 2)
893. Agrobacterium EHA101 competent cells - (reply: 1)
894. restriction digestion against colony PCR - (reply: 1)
895. BAC for transgenic mice - (reply: 6)
896. Tag proteins for CO-IP and confocal - (reply: 1)
897. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
898. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
899. how long for a double digestion - (reply: 2)
900. Problems with Site-Directed Mutagenesis - (reply: 2)