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Top : New Forum Archives (2009-): : Molecular-Cloning
871. very small insert and a very ling vector - (reply: 1)
872. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
873. cDNA clone - (reply: 1)
874. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
875. why these guys use T7 instead of CMV? - for transfection (reply: 2)
876. Electroporation - no colonies (reply: 1)
877. enzymes inactivation - (reply: 3)
878. help: why plasmid yield dropped ever since the first culture - (reply: 2)
879. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
880. miRNA cloning - (reply: 2)
881. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
882. Single-digested vector shifts downward on gel - (reply: 1)
883. problem in digestion - (reply: 1)
884. Cloning does not work - (reply: 4)
885. Small Colonies After Transformation - (reply: 5)
886. Ligation and transformation problem - (reply: 2)
887. Agrobacterium EHA101 competent cells - (reply: 1)
888. restriction digestion against colony PCR - (reply: 1)
889. BAC for transgenic mice - (reply: 6)
890. Tag proteins for CO-IP and confocal - (reply: 1)
891. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
892. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
893. how long for a double digestion - (reply: 2)
894. Problems with Site-Directed Mutagenesis - (reply: 2)
895. blunt and sticky end clonning temperature - (reply: 2)
896. Most common mistakes - What mistakes are made during cloning? (reply: 7)
897. Luciferase Assay Stim. Concentration? - (reply: 1)
898. Cloning direction / orientation? - (reply: 2)
899. Question about plasmid midiprep - (reply: 1)
900. Does a polyA sequence need to be put in-frame? - (reply: 1)