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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
872. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
873. how long for a double digestion - (reply: 2)
874. Problems with Site-Directed Mutagenesis - (reply: 2)
875. blunt and sticky end clonning temperature - (reply: 2)
876. Most common mistakes - What mistakes are made during cloning? (reply: 7)
877. Luciferase Assay Stim. Concentration? - (reply: 1)
878. Cloning direction / orientation? - (reply: 2)
879. Question about plasmid midiprep - (reply: 1)
880. Does a polyA sequence need to be put in-frame? - (reply: 1)
881. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
882. Problem on growing up bugs - (reply: 8)
883. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
884. pSIM in BL21(DE3) - (reply: 2)
885. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
886. Weird problem in invitromutagenesis - (reply: 3)
887. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
888. RE digestion - (reply: 4)
889. HELP!!! - (reply: 3)
890. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
891. Cloning of ITS region - (reply: 6)
892. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
893. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
894. SmaI blunt end ligation problem... - (reply: 3)
895. AAV construct? - (reply: 1)
896. No bands on agarose gel after restriction digestion. - (reply: 10)
897. inverse pcr - (reply: 2)
898. Viral cDNA Library - (reply: 3)
899. metylated cloning vector - restriction digest of the insert (reply: 2)
900. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)