Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
871. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
872. Chromosomal recombination with BL21? - (reply: 2)
873. Restriction Digestion!! - (reply: 5)
874. digestion - restriction enzymes (reply: 2)
875. How to prevent self ligation - selfligation (reply: 11)
876. what is the difference between DH10B and Top10 - (reply: 1)
877. problems encountered when working on a large plasmid - (reply: 2)
878. make a Mega plasmid mutation - (reply: 1)
879. Cloning - (reply: 1)
880. Copy-number calculation formula? - (reply: 1)
881. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
882. low melt agarose - (reply: 1)
883. restriction digest - smear - (reply: 1)
884. designing of primers - (reply: 2)
885. Propagating ccdB survival cells - (reply: 6)
886. Bicistronic vector vs "normal" vector - (reply: 5)
887. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
888. Can't seem to get insert into vector... please help - (reply: 4)
889. plasmid cloning - i need help.. can anyone answer me (reply: 5)
890. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
891. Topo cloning or pGEM-T easy ? - (reply: 3)
892. Problems with qiaquick gel purification kit - cloning (reply: 10)
893. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
894. integration of a gene per homologous recombination - (reply: 5)
895. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
896. Antarctic Phosphatase treatment - (reply: 5)
897. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
898. phage DNA - DNA wont migrate on agarose gel (reply: 3)
899. Plasmid out of BL21 for mutagensis - (reply: 3)
900. no colony growth - (reply: 4)