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Top : New Forum Archives (2009-): : Molecular-Cloning
871. why these guys use T7 instead of CMV? - for transfection (reply: 2)
872. Electroporation - no colonies (reply: 1)
873. enzymes inactivation - (reply: 3)
874. help: why plasmid yield dropped ever since the first culture - (reply: 2)
875. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
876. miRNA cloning - (reply: 2)
877. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
878. Single-digested vector shifts downward on gel - (reply: 1)
879. problem in digestion - (reply: 1)
880. Cloning does not work - (reply: 4)
881. Small Colonies After Transformation - (reply: 5)
882. Ligation and transformation problem - (reply: 2)
883. Agrobacterium EHA101 competent cells - (reply: 1)
884. restriction digestion against colony PCR - (reply: 1)
885. BAC for transgenic mice - (reply: 6)
886. Tag proteins for CO-IP and confocal - (reply: 1)
887. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
888. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
889. how long for a double digestion - (reply: 2)
890. Problems with Site-Directed Mutagenesis - (reply: 2)
891. blunt and sticky end clonning temperature - (reply: 2)
892. Most common mistakes - What mistakes are made during cloning? (reply: 7)
893. Luciferase Assay Stim. Concentration? - (reply: 1)
894. Cloning direction / orientation? - (reply: 2)
895. Question about plasmid midiprep - (reply: 1)
896. Does a polyA sequence need to be put in-frame? - (reply: 1)
897. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
898. Problem on growing up bugs - (reply: 8)
899. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
900. pSIM in BL21(DE3) - (reply: 2)