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Top : New Forum Archives (2009-): : Molecular-Cloning
871. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
872. How long can I store cleaved/digested vector? - (reply: 9)
873. Cloning of a gene containing 31 exon - (reply: 1)
874. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
875. Optimum Molar ratio for 3-way ligation - (reply: 5)
876. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
877. cloning help - (reply: 3)
878. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
879. A gene is not inserted into the Plasmid - (reply: 2)
880. Air bubbles during electroporation - (reply: 1)
881. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
882. Colonies without inserts - (reply: 9)
883. Transformants Don't Grow - (reply: 4)
884. control DNA transformation- using pGEM kit - transformation (reply: 4)
885. Topo Cloning - Understanding the principle (reply: 6)
886. pMX-PURO Vector not growing - (reply: 3)
887. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
888. how to set up the ligation - (reply: 14)
889. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
890. DH5 Alpha Transformation - (reply: 2)
891. Cloning a foreign gene into E-coli - (reply: 1)
892. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
893. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
894. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
895. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
896. restriction digest simple question - (reply: 4)
897. ligating small inert into a large vector - (reply: 4)
898. tranformation trouble using dh5alpha - (reply: 1)
899. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
900. Starting cells for making one's own competent cells - (reply: 7)