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Top : New Forum Archives (2009-): : Molecular-Cloning
691. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
692. smear restriction digest - (reply: 1)
693. How to set the Sonicator to 20%amplitude? - (reply: 3)
694. No DNA after midiprep - (reply: 2)
695. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
696. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
697. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
698. Impact of primer sequence on TA cloning - (reply: 3)
699. Maximum length of cDNA from RT reaction - (reply: 6)
700. overlapping PCR protocol - (reply: 12)
701. cloning an AT rich gene - (reply: 3)
702. Cloning two genes in the same plasmid - (reply: 4)
703. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
704. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
705. Problem: Cloning DNA shuffled band - (reply: 2)
706. shRNA to primary keratinocytes - (reply: 1)
707. Enzymetic digestion with ApaI and HincII - (reply: 2)
708. Ligation gel picture interpretation - (reply: 4)
709. strange subcloning problem - (reply: 9)
710. Cloned sequence is not predicted size by western blot - (reply: 1)
711. Ligation of insert to itself problem - (reply: 3)
712. double digest problem - (reply: 3)
713. Difficulty in expressing fusion protein - (reply: 1)
714. Plasmid recovery problem - (reply: 3)
715. Restriction Digest Problem - (reply: 5)
716. Recombination protocol needed - (reply: 2)
717. Cloning problem using pET32a vector - (reply: 3)
718. Fixing SNPs after cloning - (reply: 5)
719. Electroporated plasmid loses the insert - (reply: 8)
720. Recombination due to overselective plate? - (reply: 11)