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Top : New Forum Archives (2009-): : Molecular-Cloning
691. Impact of primer sequence on TA cloning - (reply: 3)
692. Maximum length of cDNA from RT reaction - (reply: 6)
693. overlapping PCR protocol - (reply: 12)
694. cloning an AT rich gene - (reply: 3)
695. Cloning two genes in the same plasmid - (reply: 4)
696. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
697. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
698. Problem: Cloning DNA shuffled band - (reply: 2)
699. shRNA to primary keratinocytes - (reply: 1)
700. Enzymetic digestion with ApaI and HincII - (reply: 2)
701. Ligation gel picture interpretation - (reply: 4)
702. strange subcloning problem - (reply: 9)
703. Cloned sequence is not predicted size by western blot - (reply: 1)
704. Ligation of insert to itself problem - (reply: 3)
705. double digest problem - (reply: 3)
706. Difficulty in expressing fusion protein - (reply: 1)
707. Plasmid recovery problem - (reply: 3)
708. Restriction Digest Problem - (reply: 5)
709. Recombination protocol needed - (reply: 2)
710. Cloning problem using pET32a vector - (reply: 3)
711. Fixing SNPs after cloning - (reply: 5)
712. Electroporated plasmid loses the insert - (reply: 8)
713. Recombination due to overselective plate? - (reply: 11)
714. Help with ligation - (reply: 2)
715. emty vector cloning - (reply: 3)
716. RE site on complimentary strand of plasmid? - (reply: 1)
717. LENTIVIRUS transfection efficiency - (reply: 1)
718. Double digest after cloning doesn't give gene - (reply: 6)
719. Blunt end PCR product ligation - (reply: 1)
720. Screening for clones - (reply: 2)