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Top : New Forum Archives (2009-): : Molecular-Cloning
691. Restriction Site Destruction - (reply: 1)
692. The copy number of pPICZ alpha A - (reply: 6)
693. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
694. need help with subcloning !! - (reply: 2)
695. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
696. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
697. cdna library. - (reply: 2)
698. Help in RE digestion of vector - (reply: 5)
699. bacterial transformation problem - (reply: 2)
700. Transformation - (reply: 3)
701. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
702. What does vortexing do in transformation? - (reply: 1)
703. failure in miniprep - (reply: 1)
704. Host cells for transformation - (reply: 3)
705. making recombinant protein - (reply: 2)
706. Confirming insert???? - (reply: 1)
707. how to make a recombinant protein with signal peptide - (reply: 1)
708. How to add restriction enzyme site into the DNA fragment - (reply: 3)
709. Issues with ligation - (reply: 2)
710. Ligation of double insert into pET31 failing - (reply: 1)
711. Homemade MiniPreps for Plasmid Purification - (reply: 1)
712. Agar has dried out during cultivation - (reply: 1)
713. Gateway cloning vector compatibility - (reply: 2)
714. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
715. Cloning of IL8-promoter - (reply: 3)
716. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
717. Template DNA (plasmid) concentration for PCR - (reply: 4)
718. Ligation of 16kb PCR product - (reply: 5)
719. stable transfection - (reply: 1)
720. How to choose the right plasmids for certain types of cell? - (reply: 1)