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Top : New Forum Archives (2009-): : Molecular-Cloning
691. self-ligation Anneled oligos - (reply: 7)
692. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
693. Looking for a GFP plasmid (non-amp) - (reply: 3)
694. clonning problem (recombined by-products) - (reply: 8)
695. Genecloning three types of molecules? - (reply: 11)
696. help in digesting DNA fragment using restriction enzyme - (reply: 2)
697. Tansformation fails ,how can i make it ? - (reply: 3)
698. pelB leader - how to use - (reply: 1)
699. Transformation bacteria heat chock and incubation? - (reply: 2)
700. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
701. ligation of pcr product into expression vector - (reply: 4)
702. amp plates containing xgal/iptg with expression vector - (reply: 6)
703. Strange Vector Problems - (reply: 3)
704. white colonies - (reply: 2)
705. how to design a kozak sequence in front of the start codon - (reply: 1)
706. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
707. XhoI digestion - (reply: 12)
708. Lenti vector ligation - (reply: 2)
709. Cloning from a pGEMT Easy vector - (reply: 3)
710. Distance between RE sites for double digestion - (reply: 5)
711. Plasmid compatible with pBAD24 - (reply: 1)
712. I cant get colony - (reply: 3)
713. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
714. Vector with inducible/repressible system - (reply: 1)
715. cloning problem - (reply: 9)
716. Cloning genes into plant vector - (reply: 1)
717. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
718. Three-way ligation in two steps? - (reply: 3)
719. How does circular, uncut pGEM-T migrate? - (reply: 2)
720. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)