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Top : New Forum Archives (2009-): : Molecular-Cloning
691. Smear above plasmid dna - (reply: 3)
692. restriction digestion buffers contaminated with dnase - (reply: 3)
693. Bacterial Induction - (reply: 1)
694. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
695. smear restriction digest - (reply: 1)
696. How to set the Sonicator to 20%amplitude? - (reply: 3)
697. No DNA after midiprep - (reply: 2)
698. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
699. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
700. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
701. Impact of primer sequence on TA cloning - (reply: 3)
702. Maximum length of cDNA from RT reaction - (reply: 6)
703. overlapping PCR protocol - (reply: 12)
704. cloning an AT rich gene - (reply: 3)
705. Cloning two genes in the same plasmid - (reply: 4)
706. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
707. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
708. Problem: Cloning DNA shuffled band - (reply: 2)
709. shRNA to primary keratinocytes - (reply: 1)
710. Enzymetic digestion with ApaI and HincII - (reply: 2)
711. Ligation gel picture interpretation - (reply: 4)
712. strange subcloning problem - (reply: 9)
713. Cloned sequence is not predicted size by western blot - (reply: 1)
714. Ligation of insert to itself problem - (reply: 3)
715. double digest problem - (reply: 3)
716. Difficulty in expressing fusion protein - (reply: 1)
717. Plasmid recovery problem - (reply: 3)
718. Restriction Digest Problem - (reply: 5)
719. Recombination protocol needed - (reply: 2)
720. Cloning problem using pET32a vector - (reply: 3)