Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
691. smear restriction digest - (reply: 1)
692. How to set the Sonicator to 20%amplitude? - (reply: 3)
693. No DNA after midiprep - (reply: 2)
694. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
695. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
696. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
697. Impact of primer sequence on TA cloning - (reply: 3)
698. Maximum length of cDNA from RT reaction - (reply: 6)
699. overlapping PCR protocol - (reply: 12)
700. cloning an AT rich gene - (reply: 3)
701. Cloning two genes in the same plasmid - (reply: 4)
702. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
703. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
704. Problem: Cloning DNA shuffled band - (reply: 2)
705. shRNA to primary keratinocytes - (reply: 1)
706. Enzymetic digestion with ApaI and HincII - (reply: 2)
707. Ligation gel picture interpretation - (reply: 4)
708. strange subcloning problem - (reply: 9)
709. Cloned sequence is not predicted size by western blot - (reply: 1)
710. Ligation of insert to itself problem - (reply: 3)
711. double digest problem - (reply: 3)
712. Difficulty in expressing fusion protein - (reply: 1)
713. Plasmid recovery problem - (reply: 3)
714. Restriction Digest Problem - (reply: 5)
715. Recombination protocol needed - (reply: 2)
716. Cloning problem using pET32a vector - (reply: 3)
717. Fixing SNPs after cloning - (reply: 5)
718. Electroporated plasmid loses the insert - (reply: 8)
719. Recombination due to overselective plate? - (reply: 11)
720. Help with ligation - (reply: 2)