Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
691. making recombinant protein - (reply: 2)
692. Confirming insert???? - (reply: 1)
693. how to make a recombinant protein with signal peptide - (reply: 1)
694. How to add restriction enzyme site into the DNA fragment - (reply: 3)
695. Issues with ligation - (reply: 2)
696. Ligation of double insert into pET31 failing - (reply: 1)
697. Homemade MiniPreps for Plasmid Purification - (reply: 1)
698. Agar has dried out during cultivation - (reply: 1)
699. Gateway cloning vector compatibility - (reply: 2)
700. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
701. Cloning of IL8-promoter - (reply: 3)
702. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
703. Template DNA (plasmid) concentration for PCR - (reply: 4)
704. Ligation of 16kb PCR product - (reply: 5)
705. stable transfection - (reply: 1)
706. How to choose the right plasmids for certain types of cell? - (reply: 1)
707. Ligation needed after SDM with PfuX? - (reply: 3)
708. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
709. Cloning promoter (overlap with another gene!) - (reply: 3)
710. bacterial transfeomation - (reply: 2)
711. Loss of insert? - (reply: 1)
712. Sequencing trouble - (reply: 7)
713. Platinum-A cell trasnfection toruble - (reply: 1)
714. Cloning a promoter - (reply: 2)
715. pcDNA 3.1 His Expression - (reply: 2)
716. Cloning two fragments into a vector - (reply: 3)
717. confirmation of insert in to vector - (reply: 3)
718. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
719. Question about Molar ratio - (reply: 4)
720. Ligation problem - (reply: 16)