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691. Radioactive carbon - (reply: 3)
692. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
693. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
694. Digesting large amount of plasmid - (reply: 4)
695. cleanup of digestion before ligation - (reply: 6)
696. transformation efficiency - (reply: 7)
697. transformed bacteria doesnt grow - (reply: 1)
698. TOPO Cloning - (reply: 5)
699. blunt ligation - (reply: 4)
700. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
701. About Laminin 5'UTR 's function - (reply: 1)
702. Vectors with special MCS - (reply: 5)
703. Cloning <100 bp fragments into a vector - (reply: 7)
704. Help! my plasmid size changed after ligation - (reply: 5)
705. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
706. Restriction digestion of gDNA for phage library - (reply: 5)
707. how i can get insert back? - (reply: 1)
708. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
709. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
710. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
711. mammalian expression vector - (reply: 2)
712. Electroporation doesn't like my plasmid??? - (reply: 4)
713. low yeild fo RNA after invitro transcription - (reply: 4)
714. No expresion of transgene - Retroviral vector (reply: 1)
715. plasmids - plasmids for stable cell line construction (reply: 2)
716. empty vectors more than the insert - (reply: 6)
717. Restriction enzyme digestion problem - (reply: 2)
718. low copy plasmid - how to improve the yield? (reply: 1)
719. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
720. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
692. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
693. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
694. Digesting large amount of plasmid - (reply: 4)
695. cleanup of digestion before ligation - (reply: 6)
696. transformation efficiency - (reply: 7)
697. transformed bacteria doesnt grow - (reply: 1)
698. TOPO Cloning - (reply: 5)
699. blunt ligation - (reply: 4)
700. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
701. About Laminin 5'UTR 's function - (reply: 1)
702. Vectors with special MCS - (reply: 5)
703. Cloning <100 bp fragments into a vector - (reply: 7)
704. Help! my plasmid size changed after ligation - (reply: 5)
705. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
706. Restriction digestion of gDNA for phage library - (reply: 5)
707. how i can get insert back? - (reply: 1)
708. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
709. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
710. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
711. mammalian expression vector - (reply: 2)
712. Electroporation doesn't like my plasmid??? - (reply: 4)
713. low yeild fo RNA after invitro transcription - (reply: 4)
714. No expresion of transgene - Retroviral vector (reply: 1)
715. plasmids - plasmids for stable cell line construction (reply: 2)
716. empty vectors more than the insert - (reply: 6)
717. Restriction enzyme digestion problem - (reply: 2)
718. low copy plasmid - how to improve the yield? (reply: 1)
719. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
720. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)