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Top : New Forum Archives (2009-): : Molecular-Cloning
691. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
692. Transformation - Transformation (reply: 1)
693. how to add 3XHA to the N-terminal of a protein - (reply: 5)
694. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
695. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
696. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
697. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
698. competent cells stock - (reply: 3)
699. Questions about pIRES-hrGFP1a - (reply: 1)
700. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
701. Cloning into lentiviral vector - (reply: 11)
702. cloning failure help plzzz - cloning (reply: 1)
703. use of pGEM -tT easy vectors - (reply: 2)
704. ~200kb Plasmid DNA purification is not working - (reply: 1)
705. ligating a 6 His tag to plasmid - (reply: 2)
706. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
707. ligation and transformation problem - (reply: 1)
708. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
709. Mini prep of frozen bacteria cells - (reply: 2)
710. PCR cloning problem - could not get colony (reply: 4)
711. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
712. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
713. PCR clonning - (reply: 3)
714. 2 bands in blue colony PCR - (reply: 5)
715. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
716. Introduction to vector development - Basics on vectors types and construction (reply: 1)
717. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
718. Targeted mutagenesis using a linear fragment - (reply: 1)
719. Transformation & Minimal Plates - (reply: 5)
720. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)