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Top : New Forum Archives (2009-): : Molecular-Cloning
691. problem with transient transfection - (reply: 6)
692. Recombinant construct looks smaller than empty vector - (reply: 3)
693. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
694. hygromycin concentration for e-coli selection - (reply: 1)
695. orientation of promoter into viral construct - (reply: 1)
696. problems with PCR confirmation of insert . HELP ! - (reply: 1)
697. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
698. Loss of promoter during ligation - (reply: 2)
699. Enzyme restriction - (reply: 8)
700. Colony PCR Problem!!!! - (reply: 5)
701. Removing unwanted sequence from pUC19 - (reply: 4)
702. transformation fails, bacteria grows in control plate - (reply: 6)
703. competentce cell, cloning - (reply: 2)
704. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
705. co-transformation - (reply: 3)
706. pcr clean-up fail? - (reply: 1)
707. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
708. sequential cloning of multiple PCR products - how to do? (reply: 3)
709. Cloning large gene - (reply: 3)
710. Glycosilated peptide's cDNA cloning - (reply: 3)
711. pWM91 cloning problem - (reply: 4)
712. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
713. Transformation - Transformation (reply: 1)
714. how to add 3XHA to the N-terminal of a protein - (reply: 5)
715. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
716. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
717. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
718. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
719. competent cells stock - (reply: 3)
720. Questions about pIRES-hrGFP1a - (reply: 1)