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Top : New Forum Archives (2009-): : Molecular-Cloning
691. LENTIVIRUS transfection efficiency - (reply: 1)
692. Double digest after cloning doesn't give gene - (reply: 6)
693. Blunt end PCR product ligation - (reply: 1)
694. Screening for clones - (reply: 2)
695. Plasmid isolation Qiagen - (reply: 2)
696. Restriction Site Destruction - (reply: 1)
697. The copy number of pPICZ alpha A - (reply: 6)
698. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
699. need help with subcloning !! - (reply: 2)
700. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
701. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
702. cdna library. - (reply: 2)
703. Help in RE digestion of vector - (reply: 5)
704. bacterial transformation problem - (reply: 2)
705. Transformation - (reply: 3)
706. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
707. What does vortexing do in transformation? - (reply: 1)
708. failure in miniprep - (reply: 1)
709. Host cells for transformation - (reply: 3)
710. making recombinant protein - (reply: 2)
711. Confirming insert???? - (reply: 1)
712. how to make a recombinant protein with signal peptide - (reply: 1)
713. How to add restriction enzyme site into the DNA fragment - (reply: 3)
714. Issues with ligation - (reply: 2)
715. Ligation of double insert into pET31 failing - (reply: 1)
716. Homemade MiniPreps for Plasmid Purification - (reply: 1)
717. Agar has dried out during cultivation - (reply: 1)
718. Gateway cloning vector compatibility - (reply: 2)
719. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
720. Cloning of IL8-promoter - (reply: 3)