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Top : New Forum Archives (2009-): : Molecular-Cloning
691. Ligation of 16kb PCR product - (reply: 5)
692. stable transfection - (reply: 1)
693. How to choose the right plasmids for certain types of cell? - (reply: 1)
694. Ligation needed after SDM with PfuX? - (reply: 3)
695. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
696. Cloning promoter (overlap with another gene!) - (reply: 3)
697. bacterial transfeomation - (reply: 2)
698. Loss of insert? - (reply: 1)
699. Sequencing trouble - (reply: 7)
700. Platinum-A cell trasnfection toruble - (reply: 1)
701. Cloning a promoter - (reply: 2)
702. pcDNA 3.1 His Expression - (reply: 2)
703. Cloning two fragments into a vector - (reply: 3)
704. confirmation of insert in to vector - (reply: 3)
705. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
706. Question about Molar ratio - (reply: 4)
707. Ligation problem - (reply: 16)
708. double digestion with sac1, hindIII - (reply: 3)
709. self-ligation Anneled oligos - (reply: 7)
710. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
711. Looking for a GFP plasmid (non-amp) - (reply: 3)
712. clonning problem (recombined by-products) - (reply: 8)
713. Genecloning three types of molecules? - (reply: 11)
714. help in digesting DNA fragment using restriction enzyme - (reply: 2)
715. Tansformation fails ,how can i make it ? - (reply: 3)
716. pelB leader - how to use - (reply: 1)
717. Transformation bacteria heat chock and incubation? - (reply: 2)
718. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
719. ligation of pcr product into expression vector - (reply: 4)
720. amp plates containing xgal/iptg with expression vector - (reply: 6)