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Top : New Forum Archives (2009-): : Molecular-Cloning
691. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
692. Loss of promoter during ligation - (reply: 2)
693. Enzyme restriction - (reply: 8)
694. Colony PCR Problem!!!! - (reply: 5)
695. Removing unwanted sequence from pUC19 - (reply: 4)
696. transformation fails, bacteria grows in control plate - (reply: 6)
697. competentce cell, cloning - (reply: 2)
698. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
699. co-transformation - (reply: 3)
700. pcr clean-up fail? - (reply: 1)
701. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
702. sequential cloning of multiple PCR products - how to do? (reply: 3)
703. Cloning large gene - (reply: 3)
704. Glycosilated peptide's cDNA cloning - (reply: 3)
705. pWM91 cloning problem - (reply: 4)
706. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
707. Transformation - Transformation (reply: 1)
708. how to add 3XHA to the N-terminal of a protein - (reply: 5)
709. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
710. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
711. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
712. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
713. competent cells stock - (reply: 3)
714. Questions about pIRES-hrGFP1a - (reply: 1)
715. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
716. Cloning into lentiviral vector - (reply: 11)
717. cloning failure help plzzz - cloning (reply: 1)
718. use of pGEM -tT easy vectors - (reply: 2)
719. ~200kb Plasmid DNA purification is not working - (reply: 1)
720. ligating a 6 His tag to plasmid - (reply: 2)