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Top : New Forum Archives (2009-): : Molecular-Cloning
691. Restriction Digest Problem - (reply: 5)
692. Recombination protocol needed - (reply: 2)
693. Cloning problem using pET32a vector - (reply: 3)
694. Fixing SNPs after cloning - (reply: 5)
695. Electroporated plasmid loses the insert - (reply: 8)
696. Recombination due to overselective plate? - (reply: 11)
697. Help with ligation - (reply: 2)
698. emty vector cloning - (reply: 3)
699. RE site on complimentary strand of plasmid? - (reply: 1)
700. LENTIVIRUS transfection efficiency - (reply: 1)
701. Double digest after cloning doesn't give gene - (reply: 6)
702. Blunt end PCR product ligation - (reply: 1)
703. Screening for clones - (reply: 2)
704. Plasmid isolation Qiagen - (reply: 2)
705. Restriction Site Destruction - (reply: 1)
706. The copy number of pPICZ alpha A - (reply: 6)
707. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
708. need help with subcloning !! - (reply: 2)
709. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
710. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
711. cdna library. - (reply: 2)
712. Help in RE digestion of vector - (reply: 5)
713. bacterial transformation problem - (reply: 2)
714. Transformation - (reply: 3)
715. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
716. What does vortexing do in transformation? - (reply: 1)
717. failure in miniprep - (reply: 1)
718. Host cells for transformation - (reply: 3)
719. making recombinant protein - (reply: 2)
720. Confirming insert???? - (reply: 1)