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Top : New Forum Archives (2009-): : Molecular-Cloning
1591. qPCR - urgent help (reply: 2)
1592. GFP - (reply: 2)
1593. Creating empty vector by self-ligation - (reply: 31)
1594. ligation probs - (reply: 2)
1595. Insert phosphorylation upon Klenow? - (reply: 5)
1596. confusion with different forms of plasmid - (reply: 3)
1597. an annoying cloning - (reply: 9)
1598. dpn1 digest - (reply: 5)
1599. Controls for TOPO cloning? - (reply: 1)
1600. primers fpr sequencing - (reply: 4)
1601. help with ligation/transformation - (reply: 1)
1602. ligation problem with SalI degested vector and insert - (reply: 6)
1603. IRES1 x IRES2? - (reply: 5)
1604. Restriction site - efficiency of restriction (reply: 4)
1605. lentivirus vector problem--help help - (reply: 6)
1606. A problem with deleting URA3 from S. cerevisiae - (reply: 2)
1607. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
1608. ligation - didn't got transformed colonies (reply: 1)
1609. What's this in my culture??? - Debris in liquid culture (reply: 3)
1610. assembling problem in genes - assembling problem in genes (reply: 7)
1611. tryptone soy broth - (reply: 3)
1612. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
1613. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
1614. ligation problem - (reply: 3)
1615. ligation question - three-point ligation (reply: 9)
1616. Problems cloning Hairpin vector - (reply: 3)
1617. request pIRES2-EGFP - (reply: 2)
1618. different digestion pattern for same clone - (reply: 4)
1619. Problem with DNA purification from agarose gel - (reply: 3)
1620. cloning trouble - (reply: 15)