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Top : New Forum Archives (2009-): : Molecular-Cloning
1591. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
1592. ligation - didn't got transformed colonies (reply: 1)
1593. What's this in my culture??? - Debris in liquid culture (reply: 3)
1594. assembling problem in genes - assembling problem in genes (reply: 7)
1595. tryptone soy broth - (reply: 3)
1596. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
1597. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
1598. ligation problem - (reply: 3)
1599. ligation question - three-point ligation (reply: 9)
1600. Problems cloning Hairpin vector - (reply: 3)
1601. request pIRES2-EGFP - (reply: 2)
1602. different digestion pattern for same clone - (reply: 4)
1603. Problem with DNA purification from agarose gel - (reply: 3)
1604. cloning trouble - (reply: 15)
1605. No band in the gel after ligation - (reply: 6)
1606. Troubleshooting Digest, ligation, transformation - (reply: 7)
1607. Ideal incubation time - (reply: 3)
1608. Competent DH5 alpha - (reply: 4)
1609. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1610. Quick ? about Enzyme Buffers - (reply: 3)
1611. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1612. Inserts too small after transformation - transformation (reply: 3)
1613. glycerol stocks - storage (reply: 2)
1614. growth of colonies after ligation - (reply: 2)
1615. cDNA cloning problem - (reply: 1)
1616. Any good way to remove DNase? - (reply: 5)
1617. DH5a comp cells - (reply: 5)
1618. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1619. Monocot promoters - (reply: 1)
1620. ligation problem - (reply: 11)