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Top : New Forum Archives (2009-): : Molecular-Cloning
1591. help with ligation/transformation - (reply: 1)
1592. ligation problem with SalI degested vector and insert - (reply: 6)
1593. IRES1 x IRES2? - (reply: 5)
1594. Restriction site - efficiency of restriction (reply: 4)
1595. lentivirus vector problem--help help - (reply: 6)
1596. A problem with deleting URA3 from S. cerevisiae - (reply: 2)
1597. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
1598. ligation - didn't got transformed colonies (reply: 1)
1599. What's this in my culture??? - Debris in liquid culture (reply: 3)
1600. assembling problem in genes - assembling problem in genes (reply: 7)
1601. tryptone soy broth - (reply: 3)
1602. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
1603. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
1604. ligation problem - (reply: 3)
1605. ligation question - three-point ligation (reply: 9)
1606. Problems cloning Hairpin vector - (reply: 3)
1607. request pIRES2-EGFP - (reply: 2)
1608. different digestion pattern for same clone - (reply: 4)
1609. Problem with DNA purification from agarose gel - (reply: 3)
1610. cloning trouble - (reply: 15)
1611. No band in the gel after ligation - (reply: 6)
1612. Troubleshooting Digest, ligation, transformation - (reply: 7)
1613. Ideal incubation time - (reply: 3)
1614. Competent DH5 alpha - (reply: 4)
1615. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1616. Quick ? about Enzyme Buffers - (reply: 3)
1617. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1618. Inserts too small after transformation - transformation (reply: 3)
1619. glycerol stocks - storage (reply: 2)
1620. growth of colonies after ligation - (reply: 2)