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Top : New Forum Archives (2009-): : Molecular-Cloning
1381. gfp-tagged protein not expressing- help! - (reply: 3)
1382. anyone working currently on gateway cloning???? - (reply: 1)
1383. Dpn1 enzyme - (reply: 2)
1384. mutagenesis expert come in~ - (reply: 11)
1385. Subcloning - (reply: 3)
1386. Sequencing different colonies - (reply: 5)
1387. detection of restriction? - (reply: 1)
1388. problem in pcr and purification - (reply: 3)
1389. bacterial stock preparation - (reply: 3)
1390. Problem with site directed mutagenesis - (reply: 7)
1391. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1392. Forgot to add X-gal/IPTG - (reply: 8)
1393. after re-isolation of vector, get single band - (reply: 4)
1394. Subcloning into pMIR-REPORT-luc - (reply: 7)
1395. Restriction digestion troubleshooting - (reply: 10)
1396. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1397. Which digestion enzyme? - (reply: 1)
1398. Cloning big fragment into smaller vector - (reply: 3)
1399. from where can i get the P1 phage - (reply: 1)
1400. multi way ligation or long range pcr? - (reply: 1)
1401. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1402. list of sequence to be added before RE site - (reply: 3)
1403. plasmid, BAC, or cosmid? - (reply: 3)
1404. Wierd trouble - dunno where my insert is?? (reply: 3)
1405. Large Molecular weight DNA Extarction - (reply: 7)
1406. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1407. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1408. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1409. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1410. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)