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Top : New Forum Archives (2009-): : Molecular-Cloning
1381. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1382. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1383. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1384. Vector sequence included in (PCR) amplified insert - (reply: 2)
1385. COMPETENT CELL PREPARATION - (reply: 6)
1386. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1387. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1388. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1389. T A cloning problems - (reply: 3)
1390. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1391. Maximum recovery time after electroporation - (reply: 2)
1392. pCLBABE puro retriviral vector - (reply: 1)
1393. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1394. Blunt-end cloning problem - (reply: 6)
1395. Supercoiling blocking restriction site? - (reply: 6)
1396. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1397. problem in gene cloning - (reply: 4)
1398. Odd point mutations - (reply: 23)
1399. Plasmid linearization by PCR - (reply: 4)
1400. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1401. can't make the cloning work - (reply: 3)
1402. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1403. cloning help - (reply: 9)
1404. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1405. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1406. Ligation trouble continues - subcloning ligation failure (reply: 9)
1407. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1408. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1409. colony PCR - (reply: 8)
1410. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)