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Top : New Forum Archives (2009-): : Molecular-Cloning
1381. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1382. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1383. Truncated sequence - (reply: 1)
1384. gateway cloning - (reply: 3)
1385. Purifying and cloning 69nt product - (reply: 5)
1386. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1387. ligatin issues - (reply: 1)
1388. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1389. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1390. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1391. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1392. A gateway cloning expression vector question - (reply: 1)
1393. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1394. Promoter/gene cloning question - (reply: 7)
1395. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1396. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1397. vivo expression technology - ivet (reply: 25)
1398. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
1399. insert is missing after TA cloning - TA cloning (reply: 6)
1400. glycerol stock - shelf life (reply: 5)
1401. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1402. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1403. cloning problem - molecular cloning (reply: 1)
1404. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1405. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1406. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1407. choosing restriction sites - (reply: 5)
1408. Tag insertion - (reply: 5)
1409. Transduction - (reply: 3)
1410. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)