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Top : New Forum Archives (2009-): : Molecular-Cloning
1381. multi way ligation or long range pcr? - (reply: 1)
1382. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1383. list of sequence to be added before RE site - (reply: 3)
1384. plasmid, BAC, or cosmid? - (reply: 3)
1385. Wierd trouble - dunno where my insert is?? (reply: 3)
1386. Large Molecular weight DNA Extarction - (reply: 7)
1387. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1388. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1389. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1390. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1391. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1392. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1393. Vector sequence included in (PCR) amplified insert - (reply: 2)
1394. COMPETENT CELL PREPARATION - (reply: 6)
1395. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1396. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1397. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1398. T A cloning problems - (reply: 3)
1399. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1400. Maximum recovery time after electroporation - (reply: 2)
1401. pCLBABE puro retriviral vector - (reply: 1)
1402. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1403. Blunt-end cloning problem - (reply: 6)
1404. Supercoiling blocking restriction site? - (reply: 6)
1405. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1406. problem in gene cloning - (reply: 4)
1407. Odd point mutations - (reply: 23)
1408. Plasmid linearization by PCR - (reply: 4)
1409. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1410. can't make the cloning work - (reply: 3)