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Top : New Forum Archives (2009-): : Molecular-Cloning
1381. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1382. Forgot to add X-gal/IPTG - (reply: 8)
1383. after re-isolation of vector, get single band - (reply: 4)
1384. Subcloning into pMIR-REPORT-luc - (reply: 7)
1385. Restriction digestion troubleshooting - (reply: 10)
1386. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1387. Which digestion enzyme? - (reply: 1)
1388. Cloning big fragment into smaller vector - (reply: 3)
1389. from where can i get the P1 phage - (reply: 1)
1390. multi way ligation or long range pcr? - (reply: 1)
1391. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1392. list of sequence to be added before RE site - (reply: 3)
1393. plasmid, BAC, or cosmid? - (reply: 3)
1394. Wierd trouble - dunno where my insert is?? (reply: 3)
1395. Large Molecular weight DNA Extarction - (reply: 7)
1396. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1397. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1398. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1399. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1400. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1401. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1402. Vector sequence included in (PCR) amplified insert - (reply: 2)
1403. COMPETENT CELL PREPARATION - (reply: 6)
1404. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1405. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1406. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1407. T A cloning problems - (reply: 3)
1408. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1409. Maximum recovery time after electroporation - (reply: 2)
1410. pCLBABE puro retriviral vector - (reply: 1)