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Top : New Forum Archives (2009-): : Molecular-Cloning
1381. Help!!! - Unsure of problem (reply: 2)
1382. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
1383. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
1384. Site directed mutagenesis - Site directed mutagenesis (reply: 1)
1385. DNA yield estimation - (reply: 1)
1386. Vector NTI from Invitrogen - (reply: 4)
1387. gfp-tagged protein not expressing- help! - (reply: 3)
1388. anyone working currently on gateway cloning???? - (reply: 1)
1389. Dpn1 enzyme - (reply: 2)
1390. mutagenesis expert come in~ - (reply: 11)
1391. Subcloning - (reply: 3)
1392. Sequencing different colonies - (reply: 5)
1393. detection of restriction? - (reply: 1)
1394. problem in pcr and purification - (reply: 3)
1395. bacterial stock preparation - (reply: 3)
1396. Problem with site directed mutagenesis - (reply: 7)
1397. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1398. Forgot to add X-gal/IPTG - (reply: 8)
1399. after re-isolation of vector, get single band - (reply: 4)
1400. Subcloning into pMIR-REPORT-luc - (reply: 7)
1401. Restriction digestion troubleshooting - (reply: 10)
1402. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1403. Which digestion enzyme? - (reply: 1)
1404. Cloning big fragment into smaller vector - (reply: 3)
1405. from where can i get the P1 phage - (reply: 1)
1406. multi way ligation or long range pcr? - (reply: 1)
1407. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1408. list of sequence to be added before RE site - (reply: 3)
1409. plasmid, BAC, or cosmid? - (reply: 3)
1410. Wierd trouble - dunno where my insert is?? (reply: 3)