Residues on the N terminal GST Tag - (Jan/03/2013 )
İ have a stupid question. İf i clone a HİS tag directly to my rProtein İ have no Amino Acid inbetween. But in case of GST Tag İ am getting additionally AA to my rProtein. Am İ right? How can İ avoid this.
i believe the residues you mentioned inbetween the GST and the MCS are the protease recognisition sites that enable to remove the GST fusion from the rProtien. Most of the GST vectors do hav this and this also is essential in most applications. however, the HIS tag vector also come but not always with such inclusion of residues that enable the removal of tag.
may be if you want to eliminate you just got to select the vector that doesnt have such cleavage sites.