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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
902. metagenomic library consturction - BAC vectors (reply: 1)
903. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
904. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
905. Chromosomal recombination with BL21? - (reply: 2)
906. Restriction Digestion!! - (reply: 5)
907. digestion - restriction enzymes (reply: 2)
908. How to prevent self ligation - selfligation (reply: 11)
909. what is the difference between DH10B and Top10 - (reply: 1)
910. problems encountered when working on a large plasmid - (reply: 2)
911. make a Mega plasmid mutation - (reply: 1)
912. Cloning - (reply: 1)
913. Copy-number calculation formula? - (reply: 1)
914. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
915. low melt agarose - (reply: 1)
916. restriction digest - smear - (reply: 1)
917. designing of primers - (reply: 2)
918. Propagating ccdB survival cells - (reply: 6)
919. Bicistronic vector vs "normal" vector - (reply: 5)
920. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
921. Can't seem to get insert into vector... please help - (reply: 4)
922. plasmid cloning - i need help.. can anyone answer me (reply: 5)
923. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
924. Topo cloning or pGEM-T easy ? - (reply: 3)
925. Problems with qiaquick gel purification kit - cloning (reply: 10)
926. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
927. integration of a gene per homologous recombination - (reply: 5)
928. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
929. Antarctic Phosphatase treatment - (reply: 5)
930. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)