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Top : New Forum Archives (2009-): : Molecular-Cloning
901. AAV construct? - (reply: 1)
902. No bands on agarose gel after restriction digestion. - (reply: 10)
903. inverse pcr - (reply: 2)
904. Viral cDNA Library - (reply: 3)
905. metylated cloning vector - restriction digest of the insert (reply: 2)
906. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
907. invitro mutagenesis using stragene kit - (reply: 4)
908. Secretion cloning vectors - (reply: 1)
909. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
910. complementing a double knockout in E. coli - (reply: 1)
911. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
912. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
913. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
914. which G418 concentration and treatment period should be used? - (reply: 2)
915. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
916. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
917. Chemical competent E.coli always from single colony ? - (reply: 2)
918. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
919. Strange results of cloning - insert always reverse-oriented (reply: 2)
920. electroporation - (reply: 2)
921. transfection or infection - difference? (reply: 1)
922. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
923. No colonies after transformation! - (reply: 2)
924. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
925. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
926. Help Miniprep kit SIGMA - (reply: 2)
927. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
928. A question about pBR322 and pUC ori - (reply: 3)
929. Digestion of mini-prep by mistake - (reply: 3)
930. Cloning issue - (reply: 3)