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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Bicistronic vector vs "normal" vector - (reply: 5)
902. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
903. Can't seem to get insert into vector... please help - (reply: 4)
904. plasmid cloning - i need help.. can anyone answer me (reply: 5)
905. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
906. Topo cloning or pGEM-T easy ? - (reply: 3)
907. Problems with qiaquick gel purification kit - cloning (reply: 10)
908. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
909. integration of a gene per homologous recombination - (reply: 5)
910. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
911. Antarctic Phosphatase treatment - (reply: 5)
912. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
913. phage DNA - DNA wont migrate on agarose gel (reply: 3)
914. Plasmid out of BL21 for mutagensis - (reply: 3)
915. no colony growth - (reply: 4)
916. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
917. Effect of a DNA prep on DE3 - (reply: 2)
918. Competent cell storage for long term use - (reply: 2)
919. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
920. New to sequencing, primer design - (reply: 2)
921. ta cloning problem - (reply: 2)
922. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
923. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
924. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
925. Storage of Bacterial Culture at 4 degrees - (reply: 2)
926. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
927. rna isolation - (reply: 1)
928. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
929. Molecular Cloning od Recombinant DNA - (reply: 3)
930. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)