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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
902. invitro mutagenesis using stragene kit - (reply: 4)
903. Secretion cloning vectors - (reply: 1)
904. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
905. complementing a double knockout in E. coli - (reply: 1)
906. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
907. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
908. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
909. which G418 concentration and treatment period should be used? - (reply: 2)
910. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
911. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
912. Chemical competent E.coli always from single colony ? - (reply: 2)
913. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
914. Strange results of cloning - insert always reverse-oriented (reply: 2)
915. electroporation - (reply: 2)
916. transfection or infection - difference? (reply: 1)
917. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
918. No colonies after transformation! - (reply: 2)
919. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
920. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
921. Help Miniprep kit SIGMA - (reply: 2)
922. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
923. A question about pBR322 and pUC ori - (reply: 3)
924. Digestion of mini-prep by mistake - (reply: 3)
925. Cloning issue - (reply: 3)
926. Cohesive end filling after CIAP treatment - (reply: 2)
927. Looking for Pfu Taq for high GC-content template - (reply: 7)
928. questions about transformation - (reply: 4)
929. genotype lysogen DE3 - (reply: 1)
930. Need help! - Problem with right insertion! (reply: 1)