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Top : New Forum Archives (2009-): : Molecular-Cloning
901. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
902. Strange results of cloning - insert always reverse-oriented (reply: 2)
903. electroporation - (reply: 2)
904. transfection or infection - difference? (reply: 1)
905. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
906. No colonies after transformation! - (reply: 2)
907. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
908. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
909. Help Miniprep kit SIGMA - (reply: 2)
910. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
911. A question about pBR322 and pUC ori - (reply: 3)
912. Digestion of mini-prep by mistake - (reply: 3)
913. Cloning issue - (reply: 3)
914. Cohesive end filling after CIAP treatment - (reply: 2)
915. Looking for Pfu Taq for high GC-content template - (reply: 7)
916. questions about transformation - (reply: 4)
917. genotype lysogen DE3 - (reply: 1)
918. Need help! - Problem with right insertion! (reply: 1)
919. cloning a large insert - (reply: 5)
920. Transposase contamination in cloning - (reply: 7)
921. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
922. How long can I store cleaved/digested vector? - (reply: 9)
923. Cloning of a gene containing 31 exon - (reply: 1)
924. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
925. Optimum Molar ratio for 3-way ligation - (reply: 5)
926. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
927. cloning help - (reply: 3)
928. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
929. A gene is not inserted into the Plasmid - (reply: 2)
930. Air bubbles during electroporation - (reply: 1)