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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Molecular Cloning od Recombinant DNA - (reply: 3)
902. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
903. looking for binding site - software? (reply: 1)
904. Insertion of loxp/avoiding SINE - (reply: 1)
905. Question: Ligation Problem.... - (reply: 2)
906. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
907. Topo directional cloning - pENTR/D-TOPO (reply: 1)
908. trouble in TOPO TA sequencing - (reply: 1)
909. Large DNA extraction from an agarose gel - (reply: 8)
910. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
911. primer Tm is too high, how tu get pcr product - help! (reply: 21)
912. Ligation problem, religation of backbone without any insert? - (reply: 3)
913. problem with pLK01 plasmid ligation - (reply: 2)
914. Mini prep streaking - basic question (reply: 3)
915. In-fusion joining few parts together - possible? ideas? (reply: 3)
916. TOPO cloning problem - (reply: 4)
917. How to delete a domain in a gene? - cloned plasmid (reply: 12)
918. Red/ET recombination - (reply: 2)
919. multiple origins of replication - (reply: 2)
920. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
921. Single Restriction Site cloning - Cloning (reply: 6)
922. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
923. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
924. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
925. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
926. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
927. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
928. Agarose gel electrophoresis question - (reply: 1)
929. amount of plasmid for transformation and fusion protein expression - (reply: 1)
930. My white colonies turned blue in frig! Why? - (reply: 6)