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Top : New Forum Archives (2009-): : Molecular-Cloning
901. complementing a double knockout in E. coli - (reply: 1)
902. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
903. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
904. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
905. which G418 concentration and treatment period should be used? - (reply: 2)
906. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
907. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
908. Chemical competent E.coli always from single colony ? - (reply: 2)
909. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
910. Strange results of cloning - insert always reverse-oriented (reply: 2)
911. electroporation - (reply: 2)
912. transfection or infection - difference? (reply: 1)
913. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
914. No colonies after transformation! - (reply: 2)
915. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
916. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
917. Help Miniprep kit SIGMA - (reply: 2)
918. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
919. A question about pBR322 and pUC ori - (reply: 3)
920. Digestion of mini-prep by mistake - (reply: 3)
921. Cloning issue - (reply: 3)
922. Cohesive end filling after CIAP treatment - (reply: 2)
923. Looking for Pfu Taq for high GC-content template - (reply: 7)
924. questions about transformation - (reply: 4)
925. genotype lysogen DE3 - (reply: 1)
926. Need help! - Problem with right insertion! (reply: 1)
927. cloning a large insert - (reply: 5)
928. Transposase contamination in cloning - (reply: 7)
929. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
930. How long can I store cleaved/digested vector? - (reply: 9)