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Top : New Forum Archives (2009-): : Molecular-Cloning
901. blunt and sticky end clonning temperature - (reply: 2)
902. Most common mistakes - What mistakes are made during cloning? (reply: 7)
903. Luciferase Assay Stim. Concentration? - (reply: 1)
904. Cloning direction / orientation? - (reply: 2)
905. Question about plasmid midiprep - (reply: 1)
906. Does a polyA sequence need to be put in-frame? - (reply: 1)
907. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
908. Problem on growing up bugs - (reply: 8)
909. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
910. pSIM in BL21(DE3) - (reply: 2)
911. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
912. Weird problem in invitromutagenesis - (reply: 3)
913. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
914. RE digestion - (reply: 4)
915. HELP!!! - (reply: 3)
916. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
917. Cloning of ITS region - (reply: 6)
918. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
919. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
920. SmaI blunt end ligation problem... - (reply: 3)
921. AAV construct? - (reply: 1)
922. No bands on agarose gel after restriction digestion. - (reply: 10)
923. inverse pcr - (reply: 2)
924. Viral cDNA Library - (reply: 3)
925. metylated cloning vector - restriction digest of the insert (reply: 2)
926. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
927. invitro mutagenesis using stragene kit - (reply: 4)
928. Secretion cloning vectors - (reply: 1)
929. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
930. complementing a double knockout in E. coli - (reply: 1)