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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Cloning direction / orientation? - (reply: 2)
902. Question about plasmid midiprep - (reply: 1)
903. Does a polyA sequence need to be put in-frame? - (reply: 1)
904. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
905. Problem on growing up bugs - (reply: 8)
906. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
907. pSIM in BL21(DE3) - (reply: 2)
908. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
909. Weird problem in invitromutagenesis - (reply: 3)
910. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
911. RE digestion - (reply: 4)
912. HELP!!! - (reply: 3)
913. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
914. Cloning of ITS region - (reply: 6)
915. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
916. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
917. SmaI blunt end ligation problem... - (reply: 3)
918. AAV construct? - (reply: 1)
919. No bands on agarose gel after restriction digestion. - (reply: 10)
920. inverse pcr - (reply: 2)
921. Viral cDNA Library - (reply: 3)
922. metylated cloning vector - restriction digest of the insert (reply: 2)
923. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
924. invitro mutagenesis using stragene kit - (reply: 4)
925. Secretion cloning vectors - (reply: 1)
926. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
927. complementing a double knockout in E. coli - (reply: 1)
928. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
929. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
930. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)