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Top : New Forum Archives (2009-): : Molecular-Cloning
901. SmaI blunt end ligation problem... - (reply: 3)
902. AAV construct? - (reply: 1)
903. No bands on agarose gel after restriction digestion. - (reply: 10)
904. inverse pcr - (reply: 2)
905. Viral cDNA Library - (reply: 3)
906. metylated cloning vector - restriction digest of the insert (reply: 2)
907. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
908. invitro mutagenesis using stragene kit - (reply: 4)
909. Secretion cloning vectors - (reply: 1)
910. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
911. complementing a double knockout in E. coli - (reply: 1)
912. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
913. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
914. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
915. which G418 concentration and treatment period should be used? - (reply: 2)
916. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
917. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
918. Chemical competent E.coli always from single colony ? - (reply: 2)
919. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
920. Strange results of cloning - insert always reverse-oriented (reply: 2)
921. electroporation - (reply: 2)
922. transfection or infection - difference? (reply: 1)
923. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
924. No colonies after transformation! - (reply: 2)
925. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
926. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
927. Help Miniprep kit SIGMA - (reply: 2)
928. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
929. A question about pBR322 and pUC ori - (reply: 3)
930. Digestion of mini-prep by mistake - (reply: 3)