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Top : New Forum Archives (2009-): : Molecular-Cloning
901. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
902. complementing a double knockout in E. coli - (reply: 1)
903. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
904. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
905. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
906. which G418 concentration and treatment period should be used? - (reply: 2)
907. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
908. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
909. Chemical competent E.coli always from single colony ? - (reply: 2)
910. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
911. Strange results of cloning - insert always reverse-oriented (reply: 2)
912. electroporation - (reply: 2)
913. transfection or infection - difference? (reply: 1)
914. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
915. No colonies after transformation! - (reply: 2)
916. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
917. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
918. Help Miniprep kit SIGMA - (reply: 2)
919. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
920. A question about pBR322 and pUC ori - (reply: 3)
921. Digestion of mini-prep by mistake - (reply: 3)
922. Cloning issue - (reply: 3)
923. Cohesive end filling after CIAP treatment - (reply: 2)
924. Looking for Pfu Taq for high GC-content template - (reply: 7)
925. questions about transformation - (reply: 4)
926. genotype lysogen DE3 - (reply: 1)
927. Need help! - Problem with right insertion! (reply: 1)
928. cloning a large insert - (reply: 5)
929. Transposase contamination in cloning - (reply: 7)
930. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)