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Top : New Forum Archives (2009-): : Molecular-Cloning
901. pMX-PURO Vector not growing - (reply: 3)
902. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
903. how to set up the ligation - (reply: 14)
904. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
905. DH5 Alpha Transformation - (reply: 2)
906. Cloning a foreign gene into E-coli - (reply: 1)
907. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
908. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
909. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
910. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
911. restriction digest simple question - (reply: 4)
912. ligating small inert into a large vector - (reply: 4)
913. tranformation trouble using dh5alpha - (reply: 1)
914. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
915. Starting cells for making one's own competent cells - (reply: 7)
916. 6 months... and still no positive clones! - (reply: 4)
917. Really strange problem with sequencing and plasmid purification! - (reply: 8)
918. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
919. what is site-specific integration - (reply: 2)
920. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
921. double transformations - (reply: 1)
922. Unusual band in transformants - (reply: 2)
923. ligation--pls help! - (reply: 10)
924. bizarre digestion:) - (reply: 2)
925. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
926. metagenomic library consturction - BAC vectors (reply: 1)
927. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
928. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
929. Chromosomal recombination with BL21? - (reply: 2)
930. Restriction Digestion!! - (reply: 5)