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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Digestion problem XbaI, methylation sensitive site - (reply: 3)
902. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
903. Dephosphorylation of vector - (reply: 8)
904. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
905. rna isolation - (reply: 1)
906. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
907. So many colonies! - (reply: 8)
908. Large vector cloning-recombination problem? - (reply: 1)
909. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
910. selecting recombinants - (reply: 1)
911. colony PCR for subcloning gene - (reply: 1)
912. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
913. cloning of a 20 kb insert - (reply: 2)
914. DNA quantification after gel purification - (reply: 6)
915. cloning of an operon - (reply: 2)
916. storing plasmid - -80degrees (reply: 4)
917. verifying a positive transformant - (reply: 3)
918. Need advice on cloning very small insert into a vector - (reply: 3)
919. Cloning problems i.e. Ligation - (reply: 2)
920. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
921. Radioactive carbon - (reply: 3)
922. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
923. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
924. Digesting large amount of plasmid - (reply: 4)
925. cleanup of digestion before ligation - (reply: 6)
926. transformation efficiency - (reply: 7)
927. transformed bacteria doesnt grow - (reply: 1)
928. TOPO Cloning - (reply: 5)
929. blunt ligation - (reply: 4)
930. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)