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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Transposase contamination in cloning - (reply: 7)
902. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
903. How long can I store cleaved/digested vector? - (reply: 9)
904. Cloning of a gene containing 31 exon - (reply: 1)
905. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
906. Optimum Molar ratio for 3-way ligation - (reply: 5)
907. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
908. cloning help - (reply: 3)
909. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
910. A gene is not inserted into the Plasmid - (reply: 2)
911. Air bubbles during electroporation - (reply: 1)
912. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
913. Colonies without inserts - (reply: 9)
914. Transformants Don't Grow - (reply: 4)
915. control DNA transformation- using pGEM kit - transformation (reply: 4)
916. Topo Cloning - Understanding the principle (reply: 6)
917. pMX-PURO Vector not growing - (reply: 3)
918. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
919. how to set up the ligation - (reply: 14)
920. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
921. DH5 Alpha Transformation - (reply: 2)
922. Cloning a foreign gene into E-coli - (reply: 1)
923. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
924. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
925. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
926. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
927. restriction digest simple question - (reply: 4)
928. ligating small inert into a large vector - (reply: 4)
929. tranformation trouble using dh5alpha - (reply: 1)
930. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)