Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
901. Secretion cloning vectors - (reply: 1)
902. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
903. complementing a double knockout in E. coli - (reply: 1)
904. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
905. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
906. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
907. which G418 concentration and treatment period should be used? - (reply: 2)
908. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
909. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
910. Chemical competent E.coli always from single colony ? - (reply: 2)
911. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
912. Strange results of cloning - insert always reverse-oriented (reply: 2)
913. electroporation - (reply: 2)
914. transfection or infection - difference? (reply: 1)
915. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
916. No colonies after transformation! - (reply: 2)
917. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
918. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
919. Help Miniprep kit SIGMA - (reply: 2)
920. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
921. A question about pBR322 and pUC ori - (reply: 3)
922. Digestion of mini-prep by mistake - (reply: 3)
923. Cloning issue - (reply: 3)
924. Cohesive end filling after CIAP treatment - (reply: 2)
925. Looking for Pfu Taq for high GC-content template - (reply: 7)
926. questions about transformation - (reply: 4)
927. genotype lysogen DE3 - (reply: 1)
928. Need help! - Problem with right insertion! (reply: 1)
929. cloning a large insert - (reply: 5)
930. Transposase contamination in cloning - (reply: 7)