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Top : New Forum Archives (2009-): : Molecular-Cloning
901. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
902. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
903. SmaI blunt end ligation problem... - (reply: 3)
904. AAV construct? - (reply: 1)
905. No bands on agarose gel after restriction digestion. - (reply: 10)
906. inverse pcr - (reply: 2)
907. Viral cDNA Library - (reply: 3)
908. metylated cloning vector - restriction digest of the insert (reply: 2)
909. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
910. invitro mutagenesis using stragene kit - (reply: 4)
911. Secretion cloning vectors - (reply: 1)
912. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
913. complementing a double knockout in E. coli - (reply: 1)
914. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
915. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
916. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
917. which G418 concentration and treatment period should be used? - (reply: 2)
918. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
919. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
920. Chemical competent E.coli always from single colony ? - (reply: 2)
921. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
922. Strange results of cloning - insert always reverse-oriented (reply: 2)
923. electroporation - (reply: 2)
924. transfection or infection - difference? (reply: 1)
925. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
926. No colonies after transformation! - (reply: 2)
927. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
928. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
929. Help Miniprep kit SIGMA - (reply: 2)
930. Left polymerase above fridge for more than 1 week - still working? (reply: 3)