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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
902. pSIM in BL21(DE3) - (reply: 2)
903. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
904. Weird problem in invitromutagenesis - (reply: 3)
905. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
906. RE digestion - (reply: 4)
907. HELP!!! - (reply: 3)
908. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
909. Cloning of ITS region - (reply: 6)
910. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
911. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
912. SmaI blunt end ligation problem... - (reply: 3)
913. AAV construct? - (reply: 1)
914. No bands on agarose gel after restriction digestion. - (reply: 10)
915. inverse pcr - (reply: 2)
916. Viral cDNA Library - (reply: 3)
917. metylated cloning vector - restriction digest of the insert (reply: 2)
918. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
919. invitro mutagenesis using stragene kit - (reply: 4)
920. Secretion cloning vectors - (reply: 1)
921. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
922. complementing a double knockout in E. coli - (reply: 1)
923. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
924. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
925. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
926. which G418 concentration and treatment period should be used? - (reply: 2)
927. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
928. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
929. Chemical competent E.coli always from single colony ? - (reply: 2)
930. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)