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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Viral cDNA Library - (reply: 3)
902. metylated cloning vector - restriction digest of the insert (reply: 2)
903. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
904. invitro mutagenesis using stragene kit - (reply: 4)
905. Secretion cloning vectors - (reply: 1)
906. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
907. complementing a double knockout in E. coli - (reply: 1)
908. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
909. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
910. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
911. which G418 concentration and treatment period should be used? - (reply: 2)
912. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
913. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
914. Chemical competent E.coli always from single colony ? - (reply: 2)
915. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
916. Strange results of cloning - insert always reverse-oriented (reply: 2)
917. electroporation - (reply: 2)
918. transfection or infection - difference? (reply: 1)
919. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
920. No colonies after transformation! - (reply: 2)
921. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
922. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
923. Help Miniprep kit SIGMA - (reply: 2)
924. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
925. A question about pBR322 and pUC ori - (reply: 3)
926. Digestion of mini-prep by mistake - (reply: 3)
927. Cloning issue - (reply: 3)
928. Cohesive end filling after CIAP treatment - (reply: 2)
929. Looking for Pfu Taq for high GC-content template - (reply: 7)
930. questions about transformation - (reply: 4)