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Top : New Forum Archives (2009-): : Molecular-Cloning
901. Does a polyA sequence need to be put in-frame? - (reply: 1)
902. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
903. Problem on growing up bugs - (reply: 8)
904. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
905. pSIM in BL21(DE3) - (reply: 2)
906. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
907. Weird problem in invitromutagenesis - (reply: 3)
908. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
909. RE digestion - (reply: 4)
910. HELP!!! - (reply: 3)
911. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
912. Cloning of ITS region - (reply: 6)
913. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
914. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
915. SmaI blunt end ligation problem... - (reply: 3)
916. AAV construct? - (reply: 1)
917. No bands on agarose gel after restriction digestion. - (reply: 10)
918. inverse pcr - (reply: 2)
919. Viral cDNA Library - (reply: 3)
920. metylated cloning vector - restriction digest of the insert (reply: 2)
921. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
922. invitro mutagenesis using stragene kit - (reply: 4)
923. Secretion cloning vectors - (reply: 1)
924. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
925. complementing a double knockout in E. coli - (reply: 1)
926. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
927. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
928. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
929. which G418 concentration and treatment period should be used? - (reply: 2)
930. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)