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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Cloning and expression problem - (reply: 5)
272. How do silicates adhere nucleic acid? - (reply: 1)
273. Ligation of PCR fragments - (reply: 11)
274. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
275. What plasmid sizes do you work with for SDM? - (reply: 1)
276. cloning help; high salt level in vector - (reply: 4)
277. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
278. problem with gateway cloning: missing part of insert - (reply: 3)
279. T7 and M13 primers two band amplification - (reply: 3)
280. Type of polymerases compatible for TA cloning - (reply: 7)
281. Running undigested plasmids on gel - (reply: 2)
282. Molecular Cloning Sambrook - (reply: 1)
283. What is the membrane made of in DNA purification kits - (reply: 3)
284. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
285. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
286. Removing Introns from a DNA sequence - (reply: 1)
287. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
288. without RE site in PCR product - (reply: 5)
289. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
290. excision of fragment during cloning - (reply: 4)
291. Unusual cloning issue... anyone else?? - (reply: 4)
292. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
293. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
294. How to avoid RNA contamination in DNA samples:? - (reply: 1)
295. Wavy Lines in Gel - (reply: 2)
296. Question Relating to LacZ Disruption - (reply: 4)
297. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
298. Site directed mutagenesis - (reply: 5)
299. PCR insert - in frame? - (reply: 2)
300. Whole plasmid amplification by PCR - (reply: 2)