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271. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
272. protein is not expessing in HEK 293 cells - (reply: 2)
273. Restriction problems - (reply: 4)
274. cloning a gene in to a plasmid - (reply: 5)
275. no product on gel after restriction digestion - (reply: 3)
276. Gateway technology. - (reply: 4)
277. Primer concentration for cDNA synthesis using GSP - (reply: 1)
278. cloning from cDNA but got a much shorter PCR product - (reply: 4)
279. GeneArt vs Quikchange - (reply: 1)
280. why do some colonies keep growing in the chiller? - (reply: 7)
281. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
282. Difference between types of bacterial culture broths? - (reply: 4)
283. Co-transfection with 2 plasmids - (reply: 10)
284. Low passage HEK293?? - (reply: 3)
285. Vector Digestion Problem - (reply: 4)
286. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
287. Transformation with a big plasmid - (reply: 4)
288. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
289. Three way ligation problem - (reply: 6)
290. problems with recovery of dna from gel and ligation - (reply: 5)
291. Colony PCR and digestion with KpnI - (reply: 10)
292. Greenish tinge to bacterial culture - (reply: 3)
293. SURE E. coli competent cells - (reply: 1)
294. Ligation - (reply: 20)
295. E. coli growing on plates and bot not in LB medium - (reply: 10)
296. *** Human Insulin promoter sequence*** - (reply: 2)
297. digested plasmid - (reply: 2)
298. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
299. Subcloning - RE digest and ligation and transformation - (reply: 5)
300. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
272. protein is not expessing in HEK 293 cells - (reply: 2)
273. Restriction problems - (reply: 4)
274. cloning a gene in to a plasmid - (reply: 5)
275. no product on gel after restriction digestion - (reply: 3)
276. Gateway technology. - (reply: 4)
277. Primer concentration for cDNA synthesis using GSP - (reply: 1)
278. cloning from cDNA but got a much shorter PCR product - (reply: 4)
279. GeneArt vs Quikchange - (reply: 1)
280. why do some colonies keep growing in the chiller? - (reply: 7)
281. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
282. Difference between types of bacterial culture broths? - (reply: 4)
283. Co-transfection with 2 plasmids - (reply: 10)
284. Low passage HEK293?? - (reply: 3)
285. Vector Digestion Problem - (reply: 4)
286. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
287. Transformation with a big plasmid - (reply: 4)
288. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
289. Three way ligation problem - (reply: 6)
290. problems with recovery of dna from gel and ligation - (reply: 5)
291. Colony PCR and digestion with KpnI - (reply: 10)
292. Greenish tinge to bacterial culture - (reply: 3)
293. SURE E. coli competent cells - (reply: 1)
294. Ligation - (reply: 20)
295. E. coli growing on plates and bot not in LB medium - (reply: 10)
296. *** Human Insulin promoter sequence*** - (reply: 2)
297. digested plasmid - (reply: 2)
298. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
299. Subcloning - RE digest and ligation and transformation - (reply: 5)
300. Plasmid digested with RE used to transform...what will happen? - (reply: 14)