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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Smallest mRNA length that can be translated - (reply: 3)
272. wrong insert? right insert?? - (reply: 2)
273. Help~ Incorrect constructs in cloning - (reply: 3)
274. Problems with ligation and transformation - (reply: 2)
275. Transformation of Restricted plasmid - (reply: 3)
276. Scrambled sequence? - (reply: 3)
277. Why is my insert missing from my plasmid? - (reply: 4)
278. PCR Profile for ligation - (reply: 3)
279. Blunt end is ligating to sticky end? - (reply: 2)
280. SNP in the promoter region - (reply: 1)
281. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
282. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
283. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
284. New ordered Restriction Enzymes not working, need help! - (reply: 2)
285. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
286. Transfection but no expression - (reply: 4)
287. Change nucleotide - (reply: 1)
288. Colonies grown without plasmid - (reply: 7)
289. unwanted band - (reply: 5)
290. Colony PCR positive and Digestion negative????? - (reply: 11)
291. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
292. Plasmid on blotting paper - (reply: 1)
293. Dimerization of PCR product - (reply: 4)
294. How to generate Lentivirus? - (reply: 4)
295. Do genes always need to be cloned in the "right direction"? - (reply: 3)
296. Gel purification (DNA) - (reply: 1)
297. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
298. Cloning and expression problem - (reply: 5)
299. How do silicates adhere nucleic acid? - (reply: 1)
300. Ligation of PCR fragments - (reply: 11)