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Top : New Forum Archives (2009-): : Molecular-Cloning
271. New ordered Restriction Enzymes not working, need help! - (reply: 2)
272. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
273. Transfection but no expression - (reply: 4)
274. Change nucleotide - (reply: 1)
275. Colonies grown without plasmid - (reply: 7)
276. unwanted band - (reply: 5)
277. Colony PCR positive and Digestion negative????? - (reply: 11)
278. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
279. Plasmid on blotting paper - (reply: 1)
280. Dimerization of PCR product - (reply: 4)
281. How to generate Lentivirus? - (reply: 4)
282. Do genes always need to be cloned in the "right direction"? - (reply: 3)
283. Gel purification (DNA) - (reply: 1)
284. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
285. Cloning and expression problem - (reply: 5)
286. How do silicates adhere nucleic acid? - (reply: 1)
287. Ligation of PCR fragments - (reply: 11)
288. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
289. What plasmid sizes do you work with for SDM? - (reply: 1)
290. cloning help; high salt level in vector - (reply: 4)
291. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
292. problem with gateway cloning: missing part of insert - (reply: 3)
293. T7 and M13 primers two band amplification - (reply: 3)
294. Type of polymerases compatible for TA cloning - (reply: 7)
295. Running undigested plasmids on gel - (reply: 2)
296. Molecular Cloning Sambrook - (reply: 1)
297. What is the membrane made of in DNA purification kits - (reply: 3)
298. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
299. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
300. Removing Introns from a DNA sequence - (reply: 1)