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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Incubation period after heat shock at transformation--reason? - (reply: 3)
272. PCR product sequencing - (reply: 3)
273. What is the optimal time for transfection efficiency measuring? - (reply: 1)
274. i couldn't get the band of target gene after ligation - (reply: 9)
275. Minimum homology bases needed for homologous recombination - (reply: 1)
276. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
277. Expansion of commercial competent bacterial cell lines? - (reply: 2)
278. Manipulation of target DNA fragments - (reply: 1)
279. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
280. Appearance of a salty plasmid preparation - (reply: 1)
281. origami bacteria transformation - (reply: 5)
282. Smallest mRNA length that can be translated - (reply: 3)
283. wrong insert? right insert?? - (reply: 2)
284. Help~ Incorrect constructs in cloning - (reply: 3)
285. Problems with ligation and transformation - (reply: 2)
286. Transformation of Restricted plasmid - (reply: 3)
287. Scrambled sequence? - (reply: 3)
288. Why is my insert missing from my plasmid? - (reply: 4)
289. PCR Profile for ligation - (reply: 3)
290. Blunt end is ligating to sticky end? - (reply: 2)
291. SNP in the promoter region - (reply: 1)
292. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
293. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
294. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
295. New ordered Restriction Enzymes not working, need help! - (reply: 2)
296. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
297. Transfection but no expression - (reply: 4)
298. Change nucleotide - (reply: 1)
299. Colonies grown without plasmid - (reply: 7)
300. unwanted band - (reply: 5)