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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Blunt end is ligating to sticky end? - (reply: 2)
272. SNP in the promoter region - (reply: 1)
273. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
274. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
275. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
276. New ordered Restriction Enzymes not working, need help! - (reply: 2)
277. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
278. Transfection but no expression - (reply: 4)
279. Change nucleotide - (reply: 1)
280. Colonies grown without plasmid - (reply: 7)
281. unwanted band - (reply: 5)
282. Colony PCR positive and Digestion negative????? - (reply: 11)
283. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
284. Plasmid on blotting paper - (reply: 1)
285. Dimerization of PCR product - (reply: 4)
286. How to generate Lentivirus? - (reply: 4)
287. Do genes always need to be cloned in the "right direction"? - (reply: 3)
288. Gel purification (DNA) - (reply: 1)
289. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
290. Cloning and expression problem - (reply: 5)
291. How do silicates adhere nucleic acid? - (reply: 1)
292. Ligation of PCR fragments - (reply: 11)
293. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
294. What plasmid sizes do you work with for SDM? - (reply: 1)
295. cloning help; high salt level in vector - (reply: 4)
296. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
297. problem with gateway cloning: missing part of insert - (reply: 3)
298. T7 and M13 primers two band amplification - (reply: 3)
299. Type of polymerases compatible for TA cloning - (reply: 7)
300. Running undigested plasmids on gel - (reply: 2)