|
271. Double strand cdna synthysis - (reply: 4)
272. mutagenic primers with very high GC content. - (reply: 3)
273. TA Cloning of ds cDNA - (reply: 6)
274. Promoter distance from ATG - (reply: 3)
275. Measuring Fluorescene of RFP - (reply: 7)
276. Smallest possible insert size for Ligation reaction - (reply: 2)
277. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
278. Extraction of DNA from termite gut flagellates - (reply: 7)
279. Enzymes and buffers from different companies - (reply: 6)
280. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
281. faint band after plasmid extraction - (reply: 7)
282. Adaptor ligation - (reply: 9)
283. self ligation - (reply: 1)
284. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
285. Cloning large transgenes - (reply: 2)
286. In-fusion HD cloning - (reply: 3)
287. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
288. Few colonies frm sticky end cloning - (reply: 3)
289. transformation problem - (reply: 8)
290. Problems with Digestion? - (reply: 7)
291. plasmid digestion - problem with enzymes? - (reply: 4)
292. Problem with cloning - (reply: 7)
293. double transformation problem - (reply: 3)
294. Too many negative clones using Directional TOPO Cloning - (reply: 5)
295. competent cells - (reply: 1)
296. "Easiest" sticky end combination - (reply: 3)
297. Cloning advice - (reply: 3)
298. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
299. Cloning large fragments - (reply: 9)
300. Transformation colonies does not contain insert - (reply: 3)
272. mutagenic primers with very high GC content. - (reply: 3)
273. TA Cloning of ds cDNA - (reply: 6)
274. Promoter distance from ATG - (reply: 3)
275. Measuring Fluorescene of RFP - (reply: 7)
276. Smallest possible insert size for Ligation reaction - (reply: 2)
277. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
278. Extraction of DNA from termite gut flagellates - (reply: 7)
279. Enzymes and buffers from different companies - (reply: 6)
280. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
281. faint band after plasmid extraction - (reply: 7)
282. Adaptor ligation - (reply: 9)
283. self ligation - (reply: 1)
284. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
285. Cloning large transgenes - (reply: 2)
286. In-fusion HD cloning - (reply: 3)
287. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
288. Few colonies frm sticky end cloning - (reply: 3)
289. transformation problem - (reply: 8)
290. Problems with Digestion? - (reply: 7)
291. plasmid digestion - problem with enzymes? - (reply: 4)
292. Problem with cloning - (reply: 7)
293. double transformation problem - (reply: 3)
294. Too many negative clones using Directional TOPO Cloning - (reply: 5)
295. competent cells - (reply: 1)
296. "Easiest" sticky end combination - (reply: 3)
297. Cloning advice - (reply: 3)
298. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
299. Cloning large fragments - (reply: 9)
300. Transformation colonies does not contain insert - (reply: 3)