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Top : New Forum Archives (2009-): : Molecular-Cloning
271. origami bacteria transformation - (reply: 5)
272. Smallest mRNA length that can be translated - (reply: 3)
273. wrong insert? right insert?? - (reply: 2)
274. Help~ Incorrect constructs in cloning - (reply: 3)
275. Problems with ligation and transformation - (reply: 2)
276. Transformation of Restricted plasmid - (reply: 3)
277. Scrambled sequence? - (reply: 3)
278. Why is my insert missing from my plasmid? - (reply: 4)
279. PCR Profile for ligation - (reply: 3)
280. Blunt end is ligating to sticky end? - (reply: 2)
281. SNP in the promoter region - (reply: 1)
282. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
283. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
284. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
285. New ordered Restriction Enzymes not working, need help! - (reply: 2)
286. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
287. Transfection but no expression - (reply: 4)
288. Change nucleotide - (reply: 1)
289. Colonies grown without plasmid - (reply: 7)
290. unwanted band - (reply: 5)
291. Colony PCR positive and Digestion negative????? - (reply: 11)
292. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
293. Plasmid on blotting paper - (reply: 1)
294. Dimerization of PCR product - (reply: 4)
295. How to generate Lentivirus? - (reply: 4)
296. Do genes always need to be cloned in the "right direction"? - (reply: 3)
297. Gel purification (DNA) - (reply: 1)
298. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
299. Cloning and expression problem - (reply: 5)
300. How do silicates adhere nucleic acid? - (reply: 1)