|
271. cloning pcr insert into plasmid - (reply: 2)
272. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
273. Large quantity heat shock trouble? - (reply: 3)
274. molecular cloning - (reply: 7)
275. Cannot see insert with colony pcr - (reply: 6)
276. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
277. help with a vector - (reply: 3)
278. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
279. Another cloning hell....no transformants - (reply: 1)
280. Agrobacterium LBA4404 transformation - (reply: 1)
281. TOPO TA Cloning Troubleshooting - (reply: 1)
282. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
283. Tricistronic vector - (reply: 1)
284. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
285. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
286. Directions of genes expression illustrations - (reply: 5)
287. Plasmid DNA at RT for a few days - (reply: 4)
288. TOPO TA Cloning - (reply: 1)
289. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
290. No insert in subcloning - (reply: 3)
291. Digest does not confirm presence of fragment - (reply: 5)
292. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
293. pGL3 Promoter replacing TSS bases - (reply: 1)
294. lipofectamine 2000:DNA complex stability - (reply: 2)
295. Problems with Ecoli glycerol stock - (reply: 6)
296. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
297. CIAP dilution - (reply: 5)
298. Problems in RNAi construction by using pUC19 - (reply: 3)
299. Unable to clone in E. coli cells - (reply: 3)
300. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
272. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
273. Large quantity heat shock trouble? - (reply: 3)
274. molecular cloning - (reply: 7)
275. Cannot see insert with colony pcr - (reply: 6)
276. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
277. help with a vector - (reply: 3)
278. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
279. Another cloning hell....no transformants - (reply: 1)
280. Agrobacterium LBA4404 transformation - (reply: 1)
281. TOPO TA Cloning Troubleshooting - (reply: 1)
282. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
283. Tricistronic vector - (reply: 1)
284. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
285. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
286. Directions of genes expression illustrations - (reply: 5)
287. Plasmid DNA at RT for a few days - (reply: 4)
288. TOPO TA Cloning - (reply: 1)
289. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
290. No insert in subcloning - (reply: 3)
291. Digest does not confirm presence of fragment - (reply: 5)
292. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
293. pGL3 Promoter replacing TSS bases - (reply: 1)
294. lipofectamine 2000:DNA complex stability - (reply: 2)
295. Problems with Ecoli glycerol stock - (reply: 6)
296. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
297. CIAP dilution - (reply: 5)
298. Problems in RNAi construction by using pUC19 - (reply: 3)
299. Unable to clone in E. coli cells - (reply: 3)
300. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)