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271. Suitable templates for in vitro transcription - (reply: 1)
272. Different ways to ensure the successful cloning? - (reply: 2)
273. Plasmid DNA digestion problem - (reply: 3)
274. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
275. Single codon insert - (reply: 3)
276. cloning- ligation - (reply: 3)
277. Sticky and blunt ligation issue - (reply: 6)
278. Handling DEPC under nitrogen/argon - (reply: 3)
279. Incubation period after heat shock at transformation--reason? - (reply: 3)
280. PCR product sequencing - (reply: 3)
281. What is the optimal time for transfection efficiency measuring? - (reply: 1)
282. i couldn't get the band of target gene after ligation - (reply: 9)
283. Minimum homology bases needed for homologous recombination - (reply: 1)
284. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
285. Expansion of commercial competent bacterial cell lines? - (reply: 2)
286. Manipulation of target DNA fragments - (reply: 1)
287. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
288. Appearance of a salty plasmid preparation - (reply: 1)
289. origami bacteria transformation - (reply: 5)
290. Smallest mRNA length that can be translated - (reply: 3)
291. wrong insert? right insert?? - (reply: 2)
292. Help~ Incorrect constructs in cloning - (reply: 3)
293. Problems with ligation and transformation - (reply: 2)
294. Transformation of Restricted plasmid - (reply: 3)
295. Scrambled sequence? - (reply: 3)
296. Why is my insert missing from my plasmid? - (reply: 4)
297. PCR Profile for ligation - (reply: 3)
298. Blunt end is ligating to sticky end? - (reply: 2)
299. SNP in the promoter region - (reply: 1)
300. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
272. Different ways to ensure the successful cloning? - (reply: 2)
273. Plasmid DNA digestion problem - (reply: 3)
274. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
275. Single codon insert - (reply: 3)
276. cloning- ligation - (reply: 3)
277. Sticky and blunt ligation issue - (reply: 6)
278. Handling DEPC under nitrogen/argon - (reply: 3)
279. Incubation period after heat shock at transformation--reason? - (reply: 3)
280. PCR product sequencing - (reply: 3)
281. What is the optimal time for transfection efficiency measuring? - (reply: 1)
282. i couldn't get the band of target gene after ligation - (reply: 9)
283. Minimum homology bases needed for homologous recombination - (reply: 1)
284. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
285. Expansion of commercial competent bacterial cell lines? - (reply: 2)
286. Manipulation of target DNA fragments - (reply: 1)
287. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
288. Appearance of a salty plasmid preparation - (reply: 1)
289. origami bacteria transformation - (reply: 5)
290. Smallest mRNA length that can be translated - (reply: 3)
291. wrong insert? right insert?? - (reply: 2)
292. Help~ Incorrect constructs in cloning - (reply: 3)
293. Problems with ligation and transformation - (reply: 2)
294. Transformation of Restricted plasmid - (reply: 3)
295. Scrambled sequence? - (reply: 3)
296. Why is my insert missing from my plasmid? - (reply: 4)
297. PCR Profile for ligation - (reply: 3)
298. Blunt end is ligating to sticky end? - (reply: 2)
299. SNP in the promoter region - (reply: 1)
300. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)