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271. Storage life of plasmid DNA in water - (reply: 5)
272. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
273. Site-directed mutagenesis failed - (reply: 20)
274. Insert & vector too dilute to perform ligation - (reply: 3)
275. Cloning Problems - (reply: 2)
276. Suitable templates for in vitro transcription - (reply: 1)
277. Different ways to ensure the successful cloning? - (reply: 2)
278. Plasmid DNA digestion problem - (reply: 3)
279. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
280. Single codon insert - (reply: 3)
281. cloning- ligation - (reply: 3)
282. Sticky and blunt ligation issue - (reply: 6)
283. Handling DEPC under nitrogen/argon - (reply: 3)
284. Incubation period after heat shock at transformation--reason? - (reply: 3)
285. PCR product sequencing - (reply: 3)
286. What is the optimal time for transfection efficiency measuring? - (reply: 1)
287. i couldn't get the band of target gene after ligation - (reply: 9)
288. Minimum homology bases needed for homologous recombination - (reply: 1)
289. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
290. Expansion of commercial competent bacterial cell lines? - (reply: 2)
291. Manipulation of target DNA fragments - (reply: 1)
292. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
293. Appearance of a salty plasmid preparation - (reply: 1)
294. origami bacteria transformation - (reply: 5)
295. Smallest mRNA length that can be translated - (reply: 3)
296. wrong insert? right insert?? - (reply: 2)
297. Help~ Incorrect constructs in cloning - (reply: 3)
298. Problems with ligation and transformation - (reply: 2)
299. Transformation of Restricted plasmid - (reply: 3)
300. Scrambled sequence? - (reply: 3)
272. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
273. Site-directed mutagenesis failed - (reply: 20)
274. Insert & vector too dilute to perform ligation - (reply: 3)
275. Cloning Problems - (reply: 2)
276. Suitable templates for in vitro transcription - (reply: 1)
277. Different ways to ensure the successful cloning? - (reply: 2)
278. Plasmid DNA digestion problem - (reply: 3)
279. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
280. Single codon insert - (reply: 3)
281. cloning- ligation - (reply: 3)
282. Sticky and blunt ligation issue - (reply: 6)
283. Handling DEPC under nitrogen/argon - (reply: 3)
284. Incubation period after heat shock at transformation--reason? - (reply: 3)
285. PCR product sequencing - (reply: 3)
286. What is the optimal time for transfection efficiency measuring? - (reply: 1)
287. i couldn't get the band of target gene after ligation - (reply: 9)
288. Minimum homology bases needed for homologous recombination - (reply: 1)
289. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
290. Expansion of commercial competent bacterial cell lines? - (reply: 2)
291. Manipulation of target DNA fragments - (reply: 1)
292. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
293. Appearance of a salty plasmid preparation - (reply: 1)
294. origami bacteria transformation - (reply: 5)
295. Smallest mRNA length that can be translated - (reply: 3)
296. wrong insert? right insert?? - (reply: 2)
297. Help~ Incorrect constructs in cloning - (reply: 3)
298. Problems with ligation and transformation - (reply: 2)
299. Transformation of Restricted plasmid - (reply: 3)
300. Scrambled sequence? - (reply: 3)