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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Appearance of a salty plasmid preparation - (reply: 1)
272. origami bacteria transformation - (reply: 5)
273. Smallest mRNA length that can be translated - (reply: 3)
274. wrong insert? right insert?? - (reply: 2)
275. Help~ Incorrect constructs in cloning - (reply: 3)
276. Problems with ligation and transformation - (reply: 2)
277. Transformation of Restricted plasmid - (reply: 3)
278. Scrambled sequence? - (reply: 3)
279. Why is my insert missing from my plasmid? - (reply: 4)
280. PCR Profile for ligation - (reply: 3)
281. Blunt end is ligating to sticky end? - (reply: 2)
282. SNP in the promoter region - (reply: 1)
283. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
284. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
285. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
286. New ordered Restriction Enzymes not working, need help! - (reply: 2)
287. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
288. Transfection but no expression - (reply: 4)
289. Change nucleotide - (reply: 1)
290. Colonies grown without plasmid - (reply: 7)
291. unwanted band - (reply: 5)
292. Colony PCR positive and Digestion negative????? - (reply: 11)
293. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
294. Plasmid on blotting paper - (reply: 1)
295. Dimerization of PCR product - (reply: 4)
296. How to generate Lentivirus? - (reply: 4)
297. Do genes always need to be cloned in the "right direction"? - (reply: 3)
298. Gel purification (DNA) - (reply: 1)
299. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
300. Cloning and expression problem - (reply: 5)