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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Handling DEPC under nitrogen/argon - (reply: 3)
272. Incubation period after heat shock at transformation--reason? - (reply: 3)
273. PCR product sequencing - (reply: 3)
274. What is the optimal time for transfection efficiency measuring? - (reply: 1)
275. i couldn't get the band of target gene after ligation - (reply: 9)
276. Minimum homology bases needed for homologous recombination - (reply: 1)
277. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
278. Expansion of commercial competent bacterial cell lines? - (reply: 2)
279. Manipulation of target DNA fragments - (reply: 1)
280. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
281. Appearance of a salty plasmid preparation - (reply: 1)
282. origami bacteria transformation - (reply: 5)
283. Smallest mRNA length that can be translated - (reply: 3)
284. wrong insert? right insert?? - (reply: 2)
285. Help~ Incorrect constructs in cloning - (reply: 3)
286. Problems with ligation and transformation - (reply: 2)
287. Transformation of Restricted plasmid - (reply: 3)
288. Scrambled sequence? - (reply: 3)
289. Why is my insert missing from my plasmid? - (reply: 4)
290. PCR Profile for ligation - (reply: 3)
291. Blunt end is ligating to sticky end? - (reply: 2)
292. SNP in the promoter region - (reply: 1)
293. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
294. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
295. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
296. New ordered Restriction Enzymes not working, need help! - (reply: 2)
297. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
298. Transfection but no expression - (reply: 4)
299. Change nucleotide - (reply: 1)
300. Colonies grown without plasmid - (reply: 7)