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Top : New Forum Archives (2009-): : Molecular-Cloning
271. problem in cloning - (reply: 2)
272. TA cloning: ligation problem or toxic construct? - (reply: 4)
273. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
274. Do I need to sequence after digestion? - (reply: 9)
275. quick change mutagenesis...... - (reply: 15)
276. Problem with double digestion: one enzyme is not working - (reply: 8)
277. Transient Transfection Controls (Gateway Technology) - (reply: 4)
278. Problems with crossover PCR - (reply: 2)
279. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
280. Two genes, same MCS - (reply: 1)
281. Including ATG in cloning? - (reply: 1)
282. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
283. Low copy vector for library generation - (reply: 2)
284. Can't find a recombinant after ligating - (reply: 4)
285. problems with plasmid miniprep - (reply: 8)
286. gene cloning - (reply: 1)
287. Is ligation really necessary? - (reply: 6)
288. problem with ligation of linker/adaptor - (reply: 2)
289. GFP -weak signal -why? - (reply: 1)
290. Ligation troubleshooting! Please help! - (reply: 5)
291. Truncated gene Cloning - (reply: 1)
292. Screening for ligation and transformation result - (reply: 2)
293. Problem with 3 way ligation - (reply: 2)
294. Query regarding primers for quick change mutagenesis - (reply: 3)
295. What is NEB's CutSmart Buffer? - (reply: 5)
296. Enzyme cutting: low efficiency - (reply: 5)
297. Issue with Xba1 and Dam methylation - (reply: 7)
298. Restriction site rearrangement - (reply: 1)
299. Mutation in cloning - (reply: 6)
300. Must the terminator be in frame? - (reply: 1)