Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
271. "Easiest" sticky end combination - (reply: 3)
272. Cloning advice - (reply: 3)
273. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
274. Cloning large fragments - (reply: 9)
275. Transformation colonies does not contain insert - (reply: 3)
276. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
277. Need urgent advise.. - (reply: 3)
278. Cloning Mitochondrial Targeting Sequence - (reply: 4)
279. problem in cloning - (reply: 2)
280. TA cloning: ligation problem or toxic construct? - (reply: 4)
281. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
282. Do I need to sequence after digestion? - (reply: 9)
283. quick change mutagenesis...... - (reply: 15)
284. Problem with double digestion: one enzyme is not working - (reply: 8)
285. Transient Transfection Controls (Gateway Technology) - (reply: 4)
286. Problems with crossover PCR - (reply: 2)
287. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
288. Two genes, same MCS - (reply: 1)
289. Including ATG in cloning? - (reply: 1)
290. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
291. Low copy vector for library generation - (reply: 2)
292. Can't find a recombinant after ligating - (reply: 4)
293. problems with plasmid miniprep - (reply: 8)
294. gene cloning - (reply: 1)
295. Is ligation really necessary? - (reply: 6)
296. problem with ligation of linker/adaptor - (reply: 2)
297. GFP -weak signal -why? - (reply: 1)
298. Ligation troubleshooting! Please help! - (reply: 5)
299. Truncated gene Cloning - (reply: 1)
300. Screening for ligation and transformation result - (reply: 2)