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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Problems with crossover PCR - (reply: 2)
272. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
273. Two genes, same MCS - (reply: 1)
274. Including ATG in cloning? - (reply: 1)
275. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
276. Low copy vector for library generation - (reply: 2)
277. Can't find a recombinant after ligating - (reply: 4)
278. problems with plasmid miniprep - (reply: 8)
279. gene cloning - (reply: 1)
280. Is ligation really necessary? - (reply: 6)
281. problem with ligation of linker/adaptor - (reply: 2)
282. GFP -weak signal -why? - (reply: 1)
283. Ligation troubleshooting! Please help! - (reply: 5)
284. Truncated gene Cloning - (reply: 1)
285. Screening for ligation and transformation result - (reply: 2)
286. Problem with 3 way ligation - (reply: 2)
287. Query regarding primers for quick change mutagenesis - (reply: 3)
288. What is NEB's CutSmart Buffer? - (reply: 5)
289. Enzyme cutting: low efficiency - (reply: 5)
290. Issue with Xba1 and Dam methylation - (reply: 7)
291. Restriction site rearrangement - (reply: 1)
292. Mutation in cloning - (reply: 6)
293. Must the terminator be in frame? - (reply: 1)
294. sequencing problems - (reply: 2)
295. Understand the basics of molecular cloning - (reply: 6)
296. Maintainence of transformed cells - (reply: 5)
297. Restriction digest band shift? - (reply: 1)
298. problem in cloning PCR primer design with restriction site - (reply: 4)
299. quick change mutagenesis...... - (reply: 1)
300. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)