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271. cloning- ligation - (reply: 3)
272. Sticky and blunt ligation issue - (reply: 6)
273. Handling DEPC under nitrogen/argon - (reply: 3)
274. Incubation period after heat shock at transformation--reason? - (reply: 3)
275. PCR product sequencing - (reply: 3)
276. What is the optimal time for transfection efficiency measuring? - (reply: 1)
277. i couldn't get the band of target gene after ligation - (reply: 9)
278. Minimum homology bases needed for homologous recombination - (reply: 1)
279. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
280. Expansion of commercial competent bacterial cell lines? - (reply: 2)
281. Manipulation of target DNA fragments - (reply: 1)
282. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
283. Appearance of a salty plasmid preparation - (reply: 1)
284. origami bacteria transformation - (reply: 5)
285. Smallest mRNA length that can be translated - (reply: 3)
286. wrong insert? right insert?? - (reply: 2)
287. Help~ Incorrect constructs in cloning - (reply: 3)
288. Problems with ligation and transformation - (reply: 2)
289. Transformation of Restricted plasmid - (reply: 3)
290. Scrambled sequence? - (reply: 3)
291. Why is my insert missing from my plasmid? - (reply: 4)
292. PCR Profile for ligation - (reply: 3)
293. Blunt end is ligating to sticky end? - (reply: 2)
294. SNP in the promoter region - (reply: 1)
295. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
296. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
297. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
298. New ordered Restriction Enzymes not working, need help! - (reply: 2)
299. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
300. Transfection but no expression - (reply: 4)
272. Sticky and blunt ligation issue - (reply: 6)
273. Handling DEPC under nitrogen/argon - (reply: 3)
274. Incubation period after heat shock at transformation--reason? - (reply: 3)
275. PCR product sequencing - (reply: 3)
276. What is the optimal time for transfection efficiency measuring? - (reply: 1)
277. i couldn't get the band of target gene after ligation - (reply: 9)
278. Minimum homology bases needed for homologous recombination - (reply: 1)
279. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
280. Expansion of commercial competent bacterial cell lines? - (reply: 2)
281. Manipulation of target DNA fragments - (reply: 1)
282. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
283. Appearance of a salty plasmid preparation - (reply: 1)
284. origami bacteria transformation - (reply: 5)
285. Smallest mRNA length that can be translated - (reply: 3)
286. wrong insert? right insert?? - (reply: 2)
287. Help~ Incorrect constructs in cloning - (reply: 3)
288. Problems with ligation and transformation - (reply: 2)
289. Transformation of Restricted plasmid - (reply: 3)
290. Scrambled sequence? - (reply: 3)
291. Why is my insert missing from my plasmid? - (reply: 4)
292. PCR Profile for ligation - (reply: 3)
293. Blunt end is ligating to sticky end? - (reply: 2)
294. SNP in the promoter region - (reply: 1)
295. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
296. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
297. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
298. New ordered Restriction Enzymes not working, need help! - (reply: 2)
299. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
300. Transfection but no expression - (reply: 4)