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271. Heat inactivate ligase - (reply: 5)
272. DMSO stock of overnight bacterial cultures - (reply: 1)
273. Recommended competent cell for transformation of large plasmids? - (reply: 4)
274. Prolem in screening of hygromycin concentration - (reply: 1)
275. Primers for Introduction of new restriction sites to a vector - (reply: 3)
276. problem in cloning involving partial digestion - (reply: 1)
277. Why perform restriction enzyme digestion again? - (reply: 1)
278. Cotransient Transfections DNA Requirements - (reply: 3)
279. which software to draw the scheme of cloning strategy - (reply: 5)
280. Help with restriction analysis - (reply: 1)
281. Sequencing of PCR product - (reply: 4)
282. KanR sequence - (reply: 2)
283. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
284. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
285. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
286. Plasmid digestion after transformation - (reply: 4)
287. Blunt end ligation insert:vector ratio - (reply: 1)
288. PCRed on restriction sites -- how do I purify it? - (reply: 8)
289. Puzzle about plamid and its digest, plz help - (reply: 8)
290. Smear above plasmid dna - (reply: 3)
291. restriction digestion buffers contaminated with dnase - (reply: 3)
292. Bacterial Induction - (reply: 1)
293. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
294. smear restriction digest - (reply: 1)
295. How to set the Sonicator to 20%amplitude? - (reply: 3)
296. No DNA after midiprep - (reply: 2)
297. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
298. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
299. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
300. Impact of primer sequence on TA cloning - (reply: 3)
272. DMSO stock of overnight bacterial cultures - (reply: 1)
273. Recommended competent cell for transformation of large plasmids? - (reply: 4)
274. Prolem in screening of hygromycin concentration - (reply: 1)
275. Primers for Introduction of new restriction sites to a vector - (reply: 3)
276. problem in cloning involving partial digestion - (reply: 1)
277. Why perform restriction enzyme digestion again? - (reply: 1)
278. Cotransient Transfections DNA Requirements - (reply: 3)
279. which software to draw the scheme of cloning strategy - (reply: 5)
280. Help with restriction analysis - (reply: 1)
281. Sequencing of PCR product - (reply: 4)
282. KanR sequence - (reply: 2)
283. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
284. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
285. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
286. Plasmid digestion after transformation - (reply: 4)
287. Blunt end ligation insert:vector ratio - (reply: 1)
288. PCRed on restriction sites -- how do I purify it? - (reply: 8)
289. Puzzle about plamid and its digest, plz help - (reply: 8)
290. Smear above plasmid dna - (reply: 3)
291. restriction digestion buffers contaminated with dnase - (reply: 3)
292. Bacterial Induction - (reply: 1)
293. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
294. smear restriction digest - (reply: 1)
295. How to set the Sonicator to 20%amplitude? - (reply: 3)
296. No DNA after midiprep - (reply: 2)
297. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
298. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
299. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
300. Impact of primer sequence on TA cloning - (reply: 3)