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Molecular-Cloning
271.
Storage life of plasmid DNA in water -
(reply: 5)
272.
Tet-On3G: cutting CMV promoter out of pCMV-Tet3G -
(reply: 1)
273.
Site-directed mutagenesis failed -
(reply: 20)
274.
Insert & vector too dilute to perform ligation -
(reply: 3)
275.
Cloning Problems -
(reply: 2)
276.
Suitable templates for in vitro transcription -
(reply: 1)
277.
Different ways to ensure the successful cloning? -
(reply: 2)
278.
Plasmid DNA digestion problem -
(reply: 3)
279.
pcDNA 3.1 Directional TOPO clone issues -
(reply: 18)
280.
Single codon insert -
(reply: 3)
281.
cloning- ligation -
(reply: 3)
282.
Sticky and blunt ligation issue -
(reply: 6)
283.
Handling DEPC under nitrogen/argon -
(reply: 3)
284.
Incubation period after heat shock at transformation--reason? -
(reply: 3)
285.
PCR product sequencing -
(reply: 3)
286.
What is the optimal time for transfection efficiency measuring? -
(reply: 1)
287.
i couldn't get the band of target gene after ligation -
(reply: 9)
288.
Minimum homology bases needed for homologous recombination -
(reply: 1)
289.
Building cDNA constructs to pcDNA3.1 directional TOPO vector -
(reply: 4)
290.
Expansion of commercial competent bacterial cell lines? -
(reply: 2)
291.
Manipulation of target DNA fragments -
(reply: 1)
292.
Trouble isolating plasmid from Pseudomonas aeruginosa -
(reply: 3)
293.
Appearance of a salty plasmid preparation -
(reply: 1)
294.
origami bacteria transformation -
(reply: 5)
295.
Smallest mRNA length that can be translated -
(reply: 3)
296.
wrong insert? right insert?? -
(reply: 2)
297.
Help~ Incorrect constructs in cloning -
(reply: 3)
298.
Problems with ligation and transformation -
(reply: 2)
299.
Transformation of Restricted plasmid -
(reply: 3)
300.
Scrambled sequence? -
(reply: 3)
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