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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Manipulation of target DNA fragments - (reply: 1)
272. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
273. Appearance of a salty plasmid preparation - (reply: 1)
274. origami bacteria transformation - (reply: 5)
275. Smallest mRNA length that can be translated - (reply: 3)
276. wrong insert? right insert?? - (reply: 2)
277. Help~ Incorrect constructs in cloning - (reply: 3)
278. Problems with ligation and transformation - (reply: 2)
279. Transformation of Restricted plasmid - (reply: 3)
280. Scrambled sequence? - (reply: 3)
281. Why is my insert missing from my plasmid? - (reply: 4)
282. PCR Profile for ligation - (reply: 3)
283. Blunt end is ligating to sticky end? - (reply: 2)
284. SNP in the promoter region - (reply: 1)
285. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
286. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
287. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
288. New ordered Restriction Enzymes not working, need help! - (reply: 2)
289. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
290. Transfection but no expression - (reply: 4)
291. Change nucleotide - (reply: 1)
292. Colonies grown without plasmid - (reply: 7)
293. unwanted band - (reply: 5)
294. Colony PCR positive and Digestion negative????? - (reply: 11)
295. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
296. Plasmid on blotting paper - (reply: 1)
297. Dimerization of PCR product - (reply: 4)
298. How to generate Lentivirus? - (reply: 4)
299. Do genes always need to be cloned in the "right direction"? - (reply: 3)
300. Gel purification (DNA) - (reply: 1)