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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
272. Need urgent advise.. - (reply: 3)
273. Cloning Mitochondrial Targeting Sequence - (reply: 4)
274. problem in cloning - (reply: 2)
275. TA cloning: ligation problem or toxic construct? - (reply: 4)
276. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
277. Do I need to sequence after digestion? - (reply: 9)
278. quick change mutagenesis...... - (reply: 15)
279. Problem with double digestion: one enzyme is not working - (reply: 8)
280. Transient Transfection Controls (Gateway Technology) - (reply: 4)
281. Problems with crossover PCR - (reply: 2)
282. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
283. Two genes, same MCS - (reply: 1)
284. Including ATG in cloning? - (reply: 1)
285. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
286. Low copy vector for library generation - (reply: 2)
287. Can't find a recombinant after ligating - (reply: 4)
288. problems with plasmid miniprep - (reply: 8)
289. gene cloning - (reply: 1)
290. Is ligation really necessary? - (reply: 6)
291. problem with ligation of linker/adaptor - (reply: 2)
292. GFP -weak signal -why? - (reply: 1)
293. Ligation troubleshooting! Please help! - (reply: 5)
294. Truncated gene Cloning - (reply: 1)
295. Screening for ligation and transformation result - (reply: 2)
296. Problem with 3 way ligation - (reply: 2)
297. Query regarding primers for quick change mutagenesis - (reply: 3)
298. What is NEB's CutSmart Buffer? - (reply: 5)
299. Enzyme cutting: low efficiency - (reply: 5)
300. Issue with Xba1 and Dam methylation - (reply: 7)