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Top : New Forum Archives (2009-): : Molecular-Cloning
271. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
272. Expansion of commercial competent bacterial cell lines? - (reply: 2)
273. Manipulation of target DNA fragments - (reply: 1)
274. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
275. Appearance of a salty plasmid preparation - (reply: 1)
276. origami bacteria transformation - (reply: 5)
277. Smallest mRNA length that can be translated - (reply: 3)
278. wrong insert? right insert?? - (reply: 2)
279. Help~ Incorrect constructs in cloning - (reply: 3)
280. Problems with ligation and transformation - (reply: 2)
281. Transformation of Restricted plasmid - (reply: 3)
282. Scrambled sequence? - (reply: 3)
283. Why is my insert missing from my plasmid? - (reply: 4)
284. PCR Profile for ligation - (reply: 3)
285. Blunt end is ligating to sticky end? - (reply: 2)
286. SNP in the promoter region - (reply: 1)
287. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
288. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
289. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
290. New ordered Restriction Enzymes not working, need help! - (reply: 2)
291. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
292. Transfection but no expression - (reply: 4)
293. Change nucleotide - (reply: 1)
294. Colonies grown without plasmid - (reply: 7)
295. unwanted band - (reply: 5)
296. Colony PCR positive and Digestion negative????? - (reply: 11)
297. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
298. Plasmid on blotting paper - (reply: 1)
299. Dimerization of PCR product - (reply: 4)
300. How to generate Lentivirus? - (reply: 4)