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Top : New Forum Archives (2009-): : Molecular-Cloning
271. PCR product sequencing - (reply: 3)
272. What is the optimal time for transfection efficiency measuring? - (reply: 1)
273. i couldn't get the band of target gene after ligation - (reply: 9)
274. Minimum homology bases needed for homologous recombination - (reply: 1)
275. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
276. Expansion of commercial competent bacterial cell lines? - (reply: 2)
277. Manipulation of target DNA fragments - (reply: 1)
278. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
279. Appearance of a salty plasmid preparation - (reply: 1)
280. origami bacteria transformation - (reply: 5)
281. Smallest mRNA length that can be translated - (reply: 3)
282. wrong insert? right insert?? - (reply: 2)
283. Help~ Incorrect constructs in cloning - (reply: 3)
284. Problems with ligation and transformation - (reply: 2)
285. Transformation of Restricted plasmid - (reply: 3)
286. Scrambled sequence? - (reply: 3)
287. Why is my insert missing from my plasmid? - (reply: 4)
288. PCR Profile for ligation - (reply: 3)
289. Blunt end is ligating to sticky end? - (reply: 2)
290. SNP in the promoter region - (reply: 1)
291. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
292. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
293. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
294. New ordered Restriction Enzymes not working, need help! - (reply: 2)
295. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
296. Transfection but no expression - (reply: 4)
297. Change nucleotide - (reply: 1)
298. Colonies grown without plasmid - (reply: 7)
299. unwanted band - (reply: 5)
300. Colony PCR positive and Digestion negative????? - (reply: 11)