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Top : New Forum Archives (2009-): : Molecular-Cloning
271. What is the optimal time for transfection efficiency measuring? - (reply: 1)
272. i couldn't get the band of target gene after ligation - (reply: 9)
273. Minimum homology bases needed for homologous recombination - (reply: 1)
274. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
275. Expansion of commercial competent bacterial cell lines? - (reply: 2)
276. Manipulation of target DNA fragments - (reply: 1)
277. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
278. Appearance of a salty plasmid preparation - (reply: 1)
279. origami bacteria transformation - (reply: 5)
280. Smallest mRNA length that can be translated - (reply: 3)
281. wrong insert? right insert?? - (reply: 2)
282. Help~ Incorrect constructs in cloning - (reply: 3)
283. Problems with ligation and transformation - (reply: 2)
284. Transformation of Restricted plasmid - (reply: 3)
285. Scrambled sequence? - (reply: 3)
286. Why is my insert missing from my plasmid? - (reply: 4)
287. PCR Profile for ligation - (reply: 3)
288. Blunt end is ligating to sticky end? - (reply: 2)
289. SNP in the promoter region - (reply: 1)
290. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
291. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
292. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
293. New ordered Restriction Enzymes not working, need help! - (reply: 2)
294. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
295. Transfection but no expression - (reply: 4)
296. Change nucleotide - (reply: 1)
297. Colonies grown without plasmid - (reply: 7)
298. unwanted band - (reply: 5)
299. Colony PCR positive and Digestion negative????? - (reply: 11)
300. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)