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271. i couldn't get the band of target gene after ligation - (reply: 9)
272. Minimum homology bases needed for homologous recombination - (reply: 1)
273. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
274. Expansion of commercial competent bacterial cell lines? - (reply: 2)
275. Manipulation of target DNA fragments - (reply: 1)
276. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
277. Appearance of a salty plasmid preparation - (reply: 1)
278. origami bacteria transformation - (reply: 5)
279. Smallest mRNA length that can be translated - (reply: 3)
280. wrong insert? right insert?? - (reply: 2)
281. Help~ Incorrect constructs in cloning - (reply: 3)
282. Problems with ligation and transformation - (reply: 2)
283. Transformation of Restricted plasmid - (reply: 3)
284. Scrambled sequence? - (reply: 3)
285. Why is my insert missing from my plasmid? - (reply: 4)
286. PCR Profile for ligation - (reply: 3)
287. Blunt end is ligating to sticky end? - (reply: 2)
288. SNP in the promoter region - (reply: 1)
289. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
290. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
291. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
292. New ordered Restriction Enzymes not working, need help! - (reply: 2)
293. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
294. Transfection but no expression - (reply: 4)
295. Change nucleotide - (reply: 1)
296. Colonies grown without plasmid - (reply: 7)
297. unwanted band - (reply: 5)
298. Colony PCR positive and Digestion negative????? - (reply: 11)
299. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
300. Plasmid on blotting paper - (reply: 1)
272. Minimum homology bases needed for homologous recombination - (reply: 1)
273. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
274. Expansion of commercial competent bacterial cell lines? - (reply: 2)
275. Manipulation of target DNA fragments - (reply: 1)
276. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
277. Appearance of a salty plasmid preparation - (reply: 1)
278. origami bacteria transformation - (reply: 5)
279. Smallest mRNA length that can be translated - (reply: 3)
280. wrong insert? right insert?? - (reply: 2)
281. Help~ Incorrect constructs in cloning - (reply: 3)
282. Problems with ligation and transformation - (reply: 2)
283. Transformation of Restricted plasmid - (reply: 3)
284. Scrambled sequence? - (reply: 3)
285. Why is my insert missing from my plasmid? - (reply: 4)
286. PCR Profile for ligation - (reply: 3)
287. Blunt end is ligating to sticky end? - (reply: 2)
288. SNP in the promoter region - (reply: 1)
289. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
290. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
291. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
292. New ordered Restriction Enzymes not working, need help! - (reply: 2)
293. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
294. Transfection but no expression - (reply: 4)
295. Change nucleotide - (reply: 1)
296. Colonies grown without plasmid - (reply: 7)
297. unwanted band - (reply: 5)
298. Colony PCR positive and Digestion negative????? - (reply: 11)
299. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
300. Plasmid on blotting paper - (reply: 1)