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271. Problem with Large vector & very small insert - (reply: 1)
272. Storage life of plasmid DNA in water - (reply: 5)
273. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
274. Site-directed mutagenesis failed - (reply: 20)
275. Insert & vector too dilute to perform ligation - (reply: 3)
276. Cloning Problems - (reply: 2)
277. Suitable templates for in vitro transcription - (reply: 1)
278. Different ways to ensure the successful cloning? - (reply: 2)
279. Plasmid DNA digestion problem - (reply: 3)
280. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
281. Single codon insert - (reply: 3)
282. cloning- ligation - (reply: 3)
283. Sticky and blunt ligation issue - (reply: 6)
284. Handling DEPC under nitrogen/argon - (reply: 3)
285. Incubation period after heat shock at transformation--reason? - (reply: 3)
286. PCR product sequencing - (reply: 3)
287. What is the optimal time for transfection efficiency measuring? - (reply: 1)
288. i couldn't get the band of target gene after ligation - (reply: 9)
289. Minimum homology bases needed for homologous recombination - (reply: 1)
290. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
291. Expansion of commercial competent bacterial cell lines? - (reply: 2)
292. Manipulation of target DNA fragments - (reply: 1)
293. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
294. Appearance of a salty plasmid preparation - (reply: 1)
295. origami bacteria transformation - (reply: 5)
296. Smallest mRNA length that can be translated - (reply: 3)
297. wrong insert? right insert?? - (reply: 2)
298. Help~ Incorrect constructs in cloning - (reply: 3)
299. Problems with ligation and transformation - (reply: 2)
300. Transformation of Restricted plasmid - (reply: 3)
272. Storage life of plasmid DNA in water - (reply: 5)
273. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
274. Site-directed mutagenesis failed - (reply: 20)
275. Insert & vector too dilute to perform ligation - (reply: 3)
276. Cloning Problems - (reply: 2)
277. Suitable templates for in vitro transcription - (reply: 1)
278. Different ways to ensure the successful cloning? - (reply: 2)
279. Plasmid DNA digestion problem - (reply: 3)
280. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
281. Single codon insert - (reply: 3)
282. cloning- ligation - (reply: 3)
283. Sticky and blunt ligation issue - (reply: 6)
284. Handling DEPC under nitrogen/argon - (reply: 3)
285. Incubation period after heat shock at transformation--reason? - (reply: 3)
286. PCR product sequencing - (reply: 3)
287. What is the optimal time for transfection efficiency measuring? - (reply: 1)
288. i couldn't get the band of target gene after ligation - (reply: 9)
289. Minimum homology bases needed for homologous recombination - (reply: 1)
290. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
291. Expansion of commercial competent bacterial cell lines? - (reply: 2)
292. Manipulation of target DNA fragments - (reply: 1)
293. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
294. Appearance of a salty plasmid preparation - (reply: 1)
295. origami bacteria transformation - (reply: 5)
296. Smallest mRNA length that can be translated - (reply: 3)
297. wrong insert? right insert?? - (reply: 2)
298. Help~ Incorrect constructs in cloning - (reply: 3)
299. Problems with ligation and transformation - (reply: 2)
300. Transformation of Restricted plasmid - (reply: 3)