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271. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
272. Can multiple genes follow only one promoter? - (reply: 1)
273. Restriction Digest Question - (reply: 4)
274. Problem with Large vector & very small insert - (reply: 1)
275. Storage life of plasmid DNA in water - (reply: 5)
276. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
277. Site-directed mutagenesis failed - (reply: 20)
278. Insert & vector too dilute to perform ligation - (reply: 3)
279. Cloning Problems - (reply: 2)
280. Suitable templates for in vitro transcription - (reply: 1)
281. Different ways to ensure the successful cloning? - (reply: 2)
282. Plasmid DNA digestion problem - (reply: 3)
283. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
284. Single codon insert - (reply: 3)
285. cloning- ligation - (reply: 3)
286. Sticky and blunt ligation issue - (reply: 6)
287. Handling DEPC under nitrogen/argon - (reply: 3)
288. Incubation period after heat shock at transformation--reason? - (reply: 3)
289. PCR product sequencing - (reply: 3)
290. What is the optimal time for transfection efficiency measuring? - (reply: 1)
291. i couldn't get the band of target gene after ligation - (reply: 9)
292. Minimum homology bases needed for homologous recombination - (reply: 1)
293. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
294. Expansion of commercial competent bacterial cell lines? - (reply: 2)
295. Manipulation of target DNA fragments - (reply: 1)
296. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
297. Appearance of a salty plasmid preparation - (reply: 1)
298. origami bacteria transformation - (reply: 5)
299. Smallest mRNA length that can be translated - (reply: 3)
300. wrong insert? right insert?? - (reply: 2)
272. Can multiple genes follow only one promoter? - (reply: 1)
273. Restriction Digest Question - (reply: 4)
274. Problem with Large vector & very small insert - (reply: 1)
275. Storage life of plasmid DNA in water - (reply: 5)
276. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
277. Site-directed mutagenesis failed - (reply: 20)
278. Insert & vector too dilute to perform ligation - (reply: 3)
279. Cloning Problems - (reply: 2)
280. Suitable templates for in vitro transcription - (reply: 1)
281. Different ways to ensure the successful cloning? - (reply: 2)
282. Plasmid DNA digestion problem - (reply: 3)
283. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
284. Single codon insert - (reply: 3)
285. cloning- ligation - (reply: 3)
286. Sticky and blunt ligation issue - (reply: 6)
287. Handling DEPC under nitrogen/argon - (reply: 3)
288. Incubation period after heat shock at transformation--reason? - (reply: 3)
289. PCR product sequencing - (reply: 3)
290. What is the optimal time for transfection efficiency measuring? - (reply: 1)
291. i couldn't get the band of target gene after ligation - (reply: 9)
292. Minimum homology bases needed for homologous recombination - (reply: 1)
293. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
294. Expansion of commercial competent bacterial cell lines? - (reply: 2)
295. Manipulation of target DNA fragments - (reply: 1)
296. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
297. Appearance of a salty plasmid preparation - (reply: 1)
298. origami bacteria transformation - (reply: 5)
299. Smallest mRNA length that can be translated - (reply: 3)
300. wrong insert? right insert?? - (reply: 2)