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Top : New Forum Archives (2009-): : Molecular-Cloning
271. What plasmid sizes do you work with for SDM? - (reply: 1)
272. cloning help; high salt level in vector - (reply: 4)
273. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
274. problem with gateway cloning: missing part of insert - (reply: 2)
275. T7 and M13 primers two band amplification - (reply: 3)
276. Type of polymerases compatible for TA cloning - (reply: 7)
277. Running undigested plasmids on gel - (reply: 2)
278. Molecular Cloning Sambrook - (reply: 1)
279. What is the membrane made of in DNA purification kits - (reply: 3)
280. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
281. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
282. Removing Introns from a DNA sequence - (reply: 1)
283. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
284. without RE site in PCR product - (reply: 5)
285. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
286. excision of fragment during cloning - (reply: 4)
287. Unusual cloning issue... anyone else?? - (reply: 4)
288. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
289. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
290. How to avoid RNA contamination in DNA samples:? - (reply: 1)
291. Wavy Lines in Gel - (reply: 2)
292. Question Relating to LacZ Disruption - (reply: 4)
293. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
294. Site directed mutagenesis - (reply: 5)
295. PCR insert - in frame? - (reply: 2)
296. Whole plasmid amplification by PCR - (reply: 2)
297. colony PCR after transformation - (reply: 1)
298. For promoter assay, which vector I should use? - (reply: 5)
299. Sequencing of the cloned plasmid - (reply: 5)
300. 4 way cloning into TA Vector - (reply: 10)