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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Ubiquitin promoter cloning - (reply: 1)
182. Cloning two genes in the same plasmid with one promotor - (reply: 4)
183. Sending a TOPO-construct for sequencing? - (reply: 1)
184. Is this counter-productive cloning or not? - (reply: 8)
185. Colony screening - (reply: 1)
186. Failed cloning for 1month - TOPO - (reply: 7)
187. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
188. Which method for DNA purification b4 cloning - (reply: 4)
189. Purification for TOPO - (reply: 1)
190. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
191. Failed TOPO cloning - what happened? - (reply: 9)
192. TOPO cloning after Phusion-PCR - (reply: 1)
193. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
194. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
195. Deleting 7Mbp chromosomal region - (reply: 3)
196. Electrophoresis samples and volumes - (reply: 1)
197. Gene cloning with unknown sequence - (reply: 6)
198. Checking for efficieny competent cells - (reply: 1)
199. Troubles with Fusion PCR - (reply: 1)
200. Does this look alright? - (reply: 3)
201. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
202. Cloning pDsRed-Monomer-N1 - (reply: 1)
203. forgot to heat shock - (reply: 6)
204. Ligation left for weekend at room temp - (reply: 4)
205. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
206. Problem in plasmid digestion after transformation - (reply: 4)
207. Lac Promoter activity in P. aeruginosa - (reply: 1)
208. Resctriction analysis - (reply: 5)
209. Please comment on my Cloning Plan - (reply: 1)
210. Gibson/SLIC question - (reply: 1)