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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Purpose of PolyA in cloning - (reply: 1)
182. Ligation failure? I ran some on a gel - (reply: 6)
183. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
184. Cloning into pVIVO2 vector - (reply: 8)
185. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
186. Cloning worked, but the expression vector is weird - (reply: 6)
187. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
188. site directed mutagenesis - (reply: 3)
189. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
190. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
191. restriction enzymes/sites to avoid - (reply: 4)
192. Using pBABE plasmids for regular transfection - (reply: 1)
193. Ubiquitin promoter cloning - (reply: 1)
194. Cloning two genes in the same plasmid with one promotor - (reply: 4)
195. Sending a TOPO-construct for sequencing? - (reply: 1)
196. Is this counter-productive cloning or not? - (reply: 8)
197. Colony screening - (reply: 1)
198. Failed cloning for 1month - TOPO - (reply: 7)
199. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
200. Which method for DNA purification b4 cloning - (reply: 4)
201. Purification for TOPO - (reply: 1)
202. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
203. Failed TOPO cloning - what happened? - (reply: 9)
204. TOPO cloning after Phusion-PCR - (reply: 1)
205. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
206. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
207. Deleting 7Mbp chromosomal region - (reply: 3)
208. Electrophoresis samples and volumes - (reply: 1)
209. Gene cloning with unknown sequence - (reply: 6)
210. Checking for efficieny competent cells - (reply: 1)