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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Help~ Incorrect constructs in cloning - (reply: 3)
182. Problems with ligation and transformation - (reply: 2)
183. Transformation of Restricted plasmid - (reply: 3)
184. Scrambled sequence? - (reply: 3)
185. Why is my insert missing from my plasmid? - (reply: 4)
186. PCR Profile for ligation - (reply: 3)
187. Blunt end is ligating to sticky end? - (reply: 2)
188. SNP in the promoter region - (reply: 1)
189. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
190. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
191. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
192. New ordered Restriction Enzymes not working, need help! - (reply: 2)
193. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
194. Transfection but no expression - (reply: 4)
195. Change nucleotide - (reply: 1)
196. Colonies grown without plasmid - (reply: 7)
197. unwanted band - (reply: 5)
198. Colony PCR positive and Digestion negative????? - (reply: 11)
199. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
200. Plasmid on blotting paper - (reply: 1)
201. Dimerization of PCR product - (reply: 4)
202. How to generate Lentivirus? - (reply: 4)
203. Do genes always need to be cloned in the "right direction"? - (reply: 3)
204. Gel purification (DNA) - (reply: 1)
205. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
206. Cloning and expression problem - (reply: 5)
207. How do silicates adhere nucleic acid? - (reply: 1)
208. Ligation of PCR fragments - (reply: 11)
209. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
210. What plasmid sizes do you work with for SDM? - (reply: 1)