Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
181. Help needed for Ligation/Transformation - (reply: 1)
182. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
183. ClaI restriction enzyme efficency - (reply: 1)
184. Blunt end cloning - (reply: 12)
185. sequantial digest - (reply: 25)
186. Temperature sensitivity of Plasmids - (reply: 1)
187. DMSO in restriction digest - (reply: 1)
188. Molecular cloning - (reply: 5)
189. Nuclease free water for cloning - (reply: 8)
190. Directional cloning 2bps overhang - (reply: 3)
191. I need help designing homology arms for CRISPR - (reply: 3)
192. Partial digest XhoI NotI NEB - (reply: 6)
193. removing part from plasmid - (reply: 18)
194. Start codon after promoter - (reply: 1)
195. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
196. Purpose of PolyA in cloning - (reply: 1)
197. Ligation failure? I ran some on a gel - (reply: 6)
198. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
199. Cloning into pVIVO2 vector - (reply: 8)
200. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
201. Cloning worked, but the expression vector is weird - (reply: 6)
202. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
203. site directed mutagenesis - (reply: 3)
204. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
205. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
206. restriction enzymes/sites to avoid - (reply: 4)
207. Using pBABE plasmids for regular transfection - (reply: 1)
208. Ubiquitin promoter cloning - (reply: 1)
209. Cloning two genes in the same plasmid with one promotor - (reply: 4)
210. Sending a TOPO-construct for sequencing? - (reply: 1)