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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Manipulation of target DNA fragments - (reply: 1)
182. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
183. Appearance of a salty plasmid preparation - (reply: 1)
184. origami bacteria transformation - (reply: 5)
185. Smallest mRNA length that can be translated - (reply: 3)
186. wrong insert? right insert?? - (reply: 2)
187. Help~ Incorrect constructs in cloning - (reply: 3)
188. Problems with ligation and transformation - (reply: 2)
189. Transformation of Restricted plasmid - (reply: 3)
190. Scrambled sequence? - (reply: 3)
191. Why is my insert missing from my plasmid? - (reply: 4)
192. PCR Profile for ligation - (reply: 3)
193. Blunt end is ligating to sticky end? - (reply: 2)
194. SNP in the promoter region - (reply: 1)
195. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
196. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
197. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
198. New ordered Restriction Enzymes not working, need help! - (reply: 2)
199. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
200. Transfection but no expression - (reply: 4)
201. Change nucleotide - (reply: 1)
202. Colonies grown without plasmid - (reply: 7)
203. unwanted band - (reply: 5)
204. Colony PCR positive and Digestion negative????? - (reply: 11)
205. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
206. Plasmid on blotting paper - (reply: 1)
207. Dimerization of PCR product - (reply: 4)
208. How to generate Lentivirus? - (reply: 4)
209. Do genes always need to be cloned in the "right direction"? - (reply: 3)
210. Gel purification (DNA) - (reply: 1)