Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
181. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
182. Cloning into pVIVO2 vector - (reply: 8)
183. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
184. Cloning worked, but the expression vector is weird - (reply: 6)
185. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
186. site directed mutagenesis - (reply: 3)
187. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
188. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
189. restriction enzymes/sites to avoid - (reply: 4)
190. Using pBABE plasmids for regular transfection - (reply: 1)
191. Ubiquitin promoter cloning - (reply: 1)
192. Cloning two genes in the same plasmid with one promotor - (reply: 4)
193. Sending a TOPO-construct for sequencing? - (reply: 1)
194. Is this counter-productive cloning or not? - (reply: 8)
195. Colony screening - (reply: 1)
196. Failed cloning for 1month - TOPO - (reply: 7)
197. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
198. Which method for DNA purification b4 cloning - (reply: 4)
199. Purification for TOPO - (reply: 1)
200. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
201. Failed TOPO cloning - what happened? - (reply: 9)
202. TOPO cloning after Phusion-PCR - (reply: 1)
203. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
204. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
205. Deleting 7Mbp chromosomal region - (reply: 3)
206. Electrophoresis samples and volumes - (reply: 1)
207. Gene cloning with unknown sequence - (reply: 6)
208. Checking for efficieny competent cells - (reply: 1)
209. Troubles with Fusion PCR - (reply: 1)
210. Does this look alright? - (reply: 3)