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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Cloning into pVIVO2 vector - (reply: 8)
182. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
183. Cloning worked, but the expression vector is weird - (reply: 6)
184. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
185. site directed mutagenesis - (reply: 3)
186. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
187. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
188. restriction enzymes/sites to avoid - (reply: 4)
189. Using pBABE plasmids for regular transfection - (reply: 1)
190. Ubiquitin promoter cloning - (reply: 1)
191. Cloning two genes in the same plasmid with one promotor - (reply: 4)
192. Sending a TOPO-construct for sequencing? - (reply: 1)
193. Is this counter-productive cloning or not? - (reply: 8)
194. Colony screening - (reply: 1)
195. Failed cloning for 1month - TOPO - (reply: 7)
196. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
197. Which method for DNA purification b4 cloning - (reply: 4)
198. Purification for TOPO - (reply: 1)
199. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
200. Failed TOPO cloning - what happened? - (reply: 9)
201. TOPO cloning after Phusion-PCR - (reply: 1)
202. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
203. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
204. Deleting 7Mbp chromosomal region - (reply: 3)
205. Electrophoresis samples and volumes - (reply: 1)
206. Gene cloning with unknown sequence - (reply: 6)
207. Checking for efficieny competent cells - (reply: 1)
208. Troubles with Fusion PCR - (reply: 1)
209. Does this look alright? - (reply: 3)
210. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)