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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Nuclease free water for cloning - (reply: 8)
182. Directional cloning 2bps overhang - (reply: 3)
183. I need help designing homology arms for CRISPR - (reply: 3)
184. Partial digest XhoI NotI NEB - (reply: 6)
185. removing part from plasmid - (reply: 18)
186. Start codon after promoter - (reply: 1)
187. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
188. Purpose of PolyA in cloning - (reply: 1)
189. Ligation failure? I ran some on a gel - (reply: 6)
190. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
191. Cloning into pVIVO2 vector - (reply: 8)
192. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
193. Cloning worked, but the expression vector is weird - (reply: 6)
194. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
195. site directed mutagenesis - (reply: 3)
196. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
197. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
198. restriction enzymes/sites to avoid - (reply: 4)
199. Using pBABE plasmids for regular transfection - (reply: 1)
200. Ubiquitin promoter cloning - (reply: 1)
201. Cloning two genes in the same plasmid with one promotor - (reply: 4)
202. Sending a TOPO-construct for sequencing? - (reply: 1)
203. Is this counter-productive cloning or not? - (reply: 8)
204. Colony screening - (reply: 1)
205. Failed cloning for 1month - TOPO - (reply: 7)
206. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
207. Which method for DNA purification b4 cloning - (reply: 4)
208. Purification for TOPO - (reply: 1)
209. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
210. Failed TOPO cloning - what happened? - (reply: 9)