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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Resctriction analysis - (reply: 5)
182. Please comment on my Cloning Plan - (reply: 1)
183. Gibson/SLIC question - (reply: 1)
184. How to determine where, in the genome, my transgene inserted - (reply: 4)
185. no colonies, ever! - (reply: 5)
186. fluorescent tag on membrane protein - (reply: 2)
187. How can addgene sell plasmids with patented technology - (reply: 2)
188. no insert issue - (reply: 9)
189. Considerations on the design of a transgene - (reply: 1)
190. How to obtain Vectors used in articles - (reply: 5)
191. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
192. How efficient is the 2A sequence - (reply: 2)
193. Transformation keeps failing - (reply: 2)
194. High background issue - (reply: 5)
195. Pcr primers - (reply: 7)
196. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
197. Gateway vectors - (reply: 3)
198. question on gateway - (reply: 1)
199. Cloning problem still not solved - (reply: 2)
200. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
201. Insert 30nt at C-terminal - (reply: 1)
202. How to store a ligation reaction over the weekend? - (reply: 3)
203. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
204. How homologous do homology arms need to be? - (reply: 3)
205. cloning pEGFP-N1 - (reply: 1)
206. Loss of DNA after restriction digestion cleanup - (reply: 2)
207. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
208. separating mixture of plamids - (reply: 7)
209. Kozak sequence and mammalian expression vector - (reply: 3)
210. GFP vector - (reply: 1)