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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
182. Cloning worked, but the expression vector is weird - (reply: 6)
183. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
184. site directed mutagenesis - (reply: 3)
185. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
186. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
187. restriction enzymes/sites to avoid - (reply: 4)
188. Using pBABE plasmids for regular transfection - (reply: 1)
189. Ubiquitin promoter cloning - (reply: 1)
190. Cloning two genes in the same plasmid with one promotor - (reply: 4)
191. Sending a TOPO-construct for sequencing? - (reply: 1)
192. Is this counter-productive cloning or not? - (reply: 8)
193. Colony screening - (reply: 1)
194. Failed cloning for 1month - TOPO - (reply: 7)
195. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
196. Which method for DNA purification b4 cloning - (reply: 4)
197. Purification for TOPO - (reply: 1)
198. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
199. Failed TOPO cloning - what happened? - (reply: 9)
200. TOPO cloning after Phusion-PCR - (reply: 1)
201. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
202. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
203. Deleting 7Mbp chromosomal region - (reply: 3)
204. Electrophoresis samples and volumes - (reply: 1)
205. Gene cloning with unknown sequence - (reply: 6)
206. Checking for efficieny competent cells - (reply: 1)
207. Troubles with Fusion PCR - (reply: 1)
208. Does this look alright? - (reply: 3)
209. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
210. Cloning pDsRed-Monomer-N1 - (reply: 1)