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Top : New Forum Archives (2009-): : Molecular-Cloning
181. no colonies, ever! - (reply: 5)
182. fluorescent tag on membrane protein - (reply: 2)
183. How can addgene sell plasmids with patented technology - (reply: 2)
184. no insert issue - (reply: 9)
185. Considerations on the design of a transgene - (reply: 1)
186. How to obtain Vectors used in articles - (reply: 5)
187. Are there any issues with tetracycline inducible promoters popping on all of a s - (reply: 2)
188. How efficient is the 2A sequence - (reply: 2)
189. Transformation keeps failing - (reply: 2)
190. High background issue - (reply: 5)
191. Pcr primers - (reply: 7)
192. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
193. Gateway vectors - (reply: 3)
194. question on gateway - (reply: 1)
195. Cloning problem still not solved - (reply: 2)
196. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
197. Insert 30nt at C-terminal - (reply: 1)
198. How to store a ligation reaction over the weekend? - (reply: 3)
199. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
200. How homologous do homology arms need to be? - (reply: 3)
201. cloning pEGFP-N1 - (reply: 1)
202. Loss of DNA after restriction digestion cleanup - (reply: 2)
203. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
204. separating mixture of plamids - (reply: 7)
205. Kozak sequence and mammalian expression vector - (reply: 3)
206. GFP vector - (reply: 1)
207. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
208. no colony - (reply: 3)
209. Cloning beginner and clueless! - (reply: 1)
210. Which epitope tag to use for ChIP? - (reply: 1)