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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Start codon after promoter - (reply: 1)
182. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
183. Purpose of PolyA in cloning - (reply: 1)
184. Ligation failure? I ran some on a gel - (reply: 6)
185. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
186. Cloning into pVIVO2 vector - (reply: 8)
187. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
188. Cloning worked, but the expression vector is weird - (reply: 6)
189. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
190. site directed mutagenesis - (reply: 3)
191. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
192. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
193. restriction enzymes/sites to avoid - (reply: 4)
194. Using pBABE plasmids for regular transfection - (reply: 1)
195. Ubiquitin promoter cloning - (reply: 1)
196. Cloning two genes in the same plasmid with one promotor - (reply: 4)
197. Sending a TOPO-construct for sequencing? - (reply: 1)
198. Is this counter-productive cloning or not? - (reply: 8)
199. Colony screening - (reply: 1)
200. Failed cloning for 1month - TOPO - (reply: 7)
201. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
202. Which method for DNA purification b4 cloning - (reply: 4)
203. Purification for TOPO - (reply: 1)
204. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
205. Failed TOPO cloning - what happened? - (reply: 9)
206. TOPO cloning after Phusion-PCR - (reply: 1)
207. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
208. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
209. Deleting 7Mbp chromosomal region - (reply: 3)
210. Electrophoresis samples and volumes - (reply: 1)