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Top : New Forum Archives (2009-): : Molecular-Cloning
181. DMSO in restriction digest - (reply: 1)
182. Molecular cloning - (reply: 5)
183. Nuclease free water for cloning - (reply: 8)
184. Directional cloning 2bps overhang - (reply: 3)
185. I need help designing homology arms for CRISPR - (reply: 3)
186. Partial digest XhoI NotI NEB - (reply: 6)
187. removing part from plasmid - (reply: 18)
188. Start codon after promoter - (reply: 1)
189. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
190. Purpose of PolyA in cloning - (reply: 1)
191. Ligation failure? I ran some on a gel - (reply: 6)
192. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
193. Cloning into pVIVO2 vector - (reply: 8)
194. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
195. Cloning worked, but the expression vector is weird - (reply: 6)
196. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
197. site directed mutagenesis - (reply: 3)
198. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
199. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
200. restriction enzymes/sites to avoid - (reply: 4)
201. Using pBABE plasmids for regular transfection - (reply: 1)
202. Ubiquitin promoter cloning - (reply: 1)
203. Cloning two genes in the same plasmid with one promotor - (reply: 4)
204. Sending a TOPO-construct for sequencing? - (reply: 1)
205. Is this counter-productive cloning or not? - (reply: 8)
206. Colony screening - (reply: 1)
207. Failed cloning for 1month - TOPO - (reply: 7)
208. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
209. Which method for DNA purification b4 cloning - (reply: 4)
210. Purification for TOPO - (reply: 1)