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181. *** Human Insulin promoter sequence*** - (reply: 2)
182. digested plasmid - (reply: 2)
183. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
184. Subcloning - RE digest and ligation and transformation - (reply: 5)
185. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
186. Making DNA Linkers for cloning purposes - (reply: 1)
187. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
188. Dam-/+ e. coli strains - (reply: 6)
189. cloning short genomic DNA fragments - (reply: 1)
190. finding the protein and nucleotide matches - (reply: 1)
191. DH10B with big construct question - (reply: 2)
192. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 13)
193. cloning to pET28a - (reply: 1)
194. Weird Library Transformation Results - (reply: 10)
195. Injecting mice with lentivirus - (reply: 1)
196. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
197. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
198. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
199. Ligation into a BAC? - (reply: 2)
200. oligo continually appears less on gel compared to other same load - (reply: 2)
201. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
202. plasmid tetR and tet promoter - (reply: 2)
203. unable to amplify an infectious retrovirus clone - (reply: 4)
204. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
205. No bands at all after double digestion - (reply: 1)
206. Digest for insert orientation - (reply: 4)
207. Colonies from transformation plate are not growing...:( - (reply: 5)
208. Problem with ligating a small insert (LoxP site) - (reply: 5)
209. Is the genomic DNA similar among different cell lines? - (reply: 4)
210. molecular cloning - (reply: 3)
182. digested plasmid - (reply: 2)
183. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
184. Subcloning - RE digest and ligation and transformation - (reply: 5)
185. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
186. Making DNA Linkers for cloning purposes - (reply: 1)
187. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
188. Dam-/+ e. coli strains - (reply: 6)
189. cloning short genomic DNA fragments - (reply: 1)
190. finding the protein and nucleotide matches - (reply: 1)
191. DH10B with big construct question - (reply: 2)
192. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 13)
193. cloning to pET28a - (reply: 1)
194. Weird Library Transformation Results - (reply: 10)
195. Injecting mice with lentivirus - (reply: 1)
196. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
197. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
198. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
199. Ligation into a BAC? - (reply: 2)
200. oligo continually appears less on gel compared to other same load - (reply: 2)
201. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
202. plasmid tetR and tet promoter - (reply: 2)
203. unable to amplify an infectious retrovirus clone - (reply: 4)
204. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
205. No bands at all after double digestion - (reply: 1)
206. Digest for insert orientation - (reply: 4)
207. Colonies from transformation plate are not growing...:( - (reply: 5)
208. Problem with ligating a small insert (LoxP site) - (reply: 5)
209. Is the genomic DNA similar among different cell lines? - (reply: 4)
210. molecular cloning - (reply: 3)