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Top : New Forum Archives (2009-): : Molecular-Cloning
181. How to generate Lentivirus? - (reply: 4)
182. Do genes always need to be cloned in the "right direction"? - (reply: 3)
183. Gel purification (DNA) - (reply: 1)
184. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
185. Cloning and expression problem - (reply: 5)
186. How do silicates adhere nucleic acid? - (reply: 1)
187. Ligation of PCR fragments - (reply: 11)
188. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
189. What plasmid sizes do you work with for SDM? - (reply: 1)
190. cloning help; high salt level in vector - (reply: 4)
191. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
192. problem with gateway cloning: missing part of insert - (reply: 2)
193. T7 and M13 primers two band amplification - (reply: 3)
194. Type of polymerases compatible for TA cloning - (reply: 7)
195. Running undigested plasmids on gel - (reply: 2)
196. Molecular Cloning Sambrook - (reply: 1)
197. What is the membrane made of in DNA purification kits - (reply: 3)
198. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
199. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
200. Removing Introns from a DNA sequence - (reply: 1)
201. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
202. without RE site in PCR product - (reply: 5)
203. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
204. excision of fragment during cloning - (reply: 4)
205. Unusual cloning issue... anyone else?? - (reply: 4)
206. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
207. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
208. How to avoid RNA contamination in DNA samples:? - (reply: 1)
209. Wavy Lines in Gel - (reply: 2)
210. Question Relating to LacZ Disruption - (reply: 4)