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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Change nucleotide - (reply: 1)
182. Colonies grown without plasmid - (reply: 7)
183. unwanted band - (reply: 5)
184. Colony PCR positive and Digestion negative????? - (reply: 11)
185. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
186. Plasmid on blotting paper - (reply: 1)
187. Dimerization of PCR product - (reply: 4)
188. How to generate Lentivirus? - (reply: 4)
189. Do genes always need to be cloned in the "right direction"? - (reply: 3)
190. Gel purification (DNA) - (reply: 1)
191. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
192. Cloning and expression problem - (reply: 5)
193. How do silicates adhere nucleic acid? - (reply: 1)
194. Ligation of PCR fragments - (reply: 11)
195. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
196. What plasmid sizes do you work with for SDM? - (reply: 1)
197. cloning help; high salt level in vector - (reply: 4)
198. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
199. problem with gateway cloning: missing part of insert - (reply: 2)
200. T7 and M13 primers two band amplification - (reply: 3)
201. Type of polymerases compatible for TA cloning - (reply: 7)
202. Running undigested plasmids on gel - (reply: 2)
203. Molecular Cloning Sambrook - (reply: 1)
204. What is the membrane made of in DNA purification kits - (reply: 3)
205. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
206. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
207. Removing Introns from a DNA sequence - (reply: 1)
208. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
209. without RE site in PCR product - (reply: 5)
210. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)