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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Directional cloning 2bps overhang - (reply: 3)
182. I need help designing homology arms for CRISPR - (reply: 3)
183. Partial digest XhoI NotI NEB - (reply: 6)
184. removing part from plasmid - (reply: 18)
185. Start codon after promoter - (reply: 1)
186. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
187. Purpose of PolyA in cloning - (reply: 1)
188. Ligation failure? I ran some on a gel - (reply: 6)
189. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
190. Cloning into pVIVO2 vector - (reply: 8)
191. Blunt-ends ligation reversing; therefore NO colonies ;( - (reply: 3)
192. Cloning worked, but the expression vector is weird - (reply: 6)
193. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
194. site directed mutagenesis - (reply: 3)
195. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
196. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
197. restriction enzymes/sites to avoid - (reply: 4)
198. Using pBABE plasmids for regular transfection - (reply: 1)
199. Ubiquitin promoter cloning - (reply: 1)
200. Cloning two genes in the same plasmid with one promotor - (reply: 4)
201. Sending a TOPO-construct for sequencing? - (reply: 1)
202. Is this counter-productive cloning or not? - (reply: 8)
203. Colony screening - (reply: 1)
204. Failed cloning for 1month - TOPO - (reply: 7)
205. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
206. Which method for DNA purification b4 cloning - (reply: 4)
207. Purification for TOPO - (reply: 1)
208. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
209. Failed TOPO cloning - what happened? - (reply: 9)
210. TOPO cloning after Phusion-PCR - (reply: 1)