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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Antibody against H1N1 cannot recognize epitopes - (reply: 6)
182. site directed mutagenesis - (reply: 3)
183. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
184. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
185. restriction enzymes/sites to avoid - (reply: 4)
186. Using pBABE plasmids for regular transfection - (reply: 1)
187. Ubiquitin promoter cloning - (reply: 1)
188. Cloning two genes in the same plasmid with one promotor - (reply: 4)
189. Sending a TOPO-construct for sequencing? - (reply: 1)
190. Is this counter-productive cloning or not? - (reply: 8)
191. Colony screening - (reply: 1)
192. Failed cloning for 1month - TOPO - (reply: 7)
193. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
194. Which method for DNA purification b4 cloning - (reply: 4)
195. Purification for TOPO - (reply: 1)
196. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
197. Failed TOPO cloning - what happened? - (reply: 9)
198. TOPO cloning after Phusion-PCR - (reply: 1)
199. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
200. How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
201. Deleting 7Mbp chromosomal region - (reply: 3)
202. Electrophoresis samples and volumes - (reply: 1)
203. Gene cloning with unknown sequence - (reply: 6)
204. Checking for efficieny competent cells - (reply: 1)
205. Troubles with Fusion PCR - (reply: 1)
206. Does this look alright? - (reply: 3)
207. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
208. Cloning pDsRed-Monomer-N1 - (reply: 1)
209. forgot to heat shock - (reply: 6)
210. Ligation left for weekend at room temp - (reply: 4)