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Top : New Forum Archives (2009-): : Molecular-Cloning
181. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
182. Site directed mutagenesis - (reply: 5)
183. PCR insert - in frame? - (reply: 2)
184. Whole plasmid amplification by PCR - (reply: 2)
185. colony PCR after transformation - (reply: 1)
186. For promoter assay, which vector I should use? - (reply: 5)
187. Sequencing of the cloned plasmid - (reply: 5)
188. 4 way cloning into TA Vector - (reply: 10)
189. I get band double the size what does it mean - (reply: 3)
190. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
191. Neeed Help , cloning Confirmation ?! - (reply: 5)
192. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
193. Can I quantify restricted plasmid and oligos? - (reply: 3)
194. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
195. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
196. TOPO TA ligation problems - (reply: 6)
197. Luciferase assay problem - (reply: 2)
198. Double digestion with BamHI and BglII - (reply: 4)
199. nicked linear DNA - (reply: 5)
200. Selection of E.coli competent cells - (reply: 2)
201. Double strand cdna synthysis - (reply: 4)
202. mutagenic primers with very high GC content. - (reply: 3)
203. TA Cloning of ds cDNA - (reply: 6)
204. Promoter distance from ATG - (reply: 3)
205. Measuring Fluorescene of RFP - (reply: 7)
206. Smallest possible insert size for Ligation reaction - (reply: 2)
207. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
208. Extraction of DNA from termite gut flagellates - (reply: 7)
209. Enzymes and buffers from different companies - (reply: 6)
210. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)