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Top : New Forum Archives (2009-): : Molecular-Cloning
181. High background issue - (reply: 5)
182. Pcr primers - (reply: 7)
183. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
184. Gateway vectors - (reply: 3)
185. question on gateway - (reply: 1)
186. Cloning problem still not solved - (reply: 2)
187. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
188. Insert 30nt at C-terminal - (reply: 1)
189. How to store a ligation reaction over the weekend? - (reply: 3)
190. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
191. How homologous do homology arms need to be? - (reply: 3)
192. cloning pEGFP-N1 - (reply: 1)
193. Loss of DNA after restriction digestion cleanup - (reply: 2)
194. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
195. separating mixture of plamids - (reply: 7)
196. Kozak sequence and mammalian expression vector - (reply: 3)
197. GFP vector - (reply: 1)
198. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
199. no colony - (reply: 3)
200. Cloning beginner and clueless! - (reply: 1)
201. Which epitope tag to use for ChIP? - (reply: 1)
202. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
203. Ligation-transformation...Never faced such problem ever - (reply: 6)
204. Can multiple genes follow only one promoter? - (reply: 1)
205. Restriction Digest Question - (reply: 4)
206. Problem with Large vector & very small insert - (reply: 1)
207. Storage life of plasmid DNA in water - (reply: 5)
208. Tet-On3G: cutting CMV promoter out of pCMV-Tet3G - (reply: 1)
209. Site-directed mutagenesis failed - (reply: 20)
210. Insert & vector too dilute to perform ligation - (reply: 3)