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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Molecular Cloning Sambrook - (reply: 1)
302. What is the membrane made of in DNA purification kits - (reply: 3)
303. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
304. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
305. Removing Introns from a DNA sequence - (reply: 1)
306. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
307. without RE site in PCR product - (reply: 5)
308. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
309. excision of fragment during cloning - (reply: 4)
310. Unusual cloning issue... anyone else?? - (reply: 4)
311. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
312. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
313. How to avoid RNA contamination in DNA samples:? - (reply: 1)
314. Wavy Lines in Gel - (reply: 2)
315. Question Relating to LacZ Disruption - (reply: 4)
316. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
317. Site directed mutagenesis - (reply: 5)
318. PCR insert - in frame? - (reply: 2)
319. Whole plasmid amplification by PCR - (reply: 2)
320. colony PCR after transformation - (reply: 1)
321. For promoter assay, which vector I should use? - (reply: 5)
322. Sequencing of the cloned plasmid - (reply: 5)
323. 4 way cloning into TA Vector - (reply: 10)
324. I get band double the size what does it mean - (reply: 3)
325. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
326. Neeed Help , cloning Confirmation ?! - (reply: 5)
327. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
328. Can I quantify restricted plasmid and oligos? - (reply: 3)
329. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
330. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)