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Top : New Forum Archives (2009-): : Molecular-Cloning
301. How do silicates adhere nucleic acid? - (reply: 1)
302. Ligation of PCR fragments - (reply: 11)
303. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
304. What plasmid sizes do you work with for SDM? - (reply: 1)
305. cloning help; high salt level in vector - (reply: 4)
306. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
307. problem with gateway cloning: missing part of insert - (reply: 3)
308. T7 and M13 primers two band amplification - (reply: 3)
309. Type of polymerases compatible for TA cloning - (reply: 7)
310. Running undigested plasmids on gel - (reply: 2)
311. Molecular Cloning Sambrook - (reply: 1)
312. What is the membrane made of in DNA purification kits - (reply: 3)
313. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
314. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
315. Removing Introns from a DNA sequence - (reply: 1)
316. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
317. without RE site in PCR product - (reply: 5)
318. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
319. excision of fragment during cloning - (reply: 4)
320. Unusual cloning issue... anyone else?? - (reply: 4)
321. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
322. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
323. How to avoid RNA contamination in DNA samples:? - (reply: 1)
324. Wavy Lines in Gel - (reply: 2)
325. Question Relating to LacZ Disruption - (reply: 4)
326. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
327. Site directed mutagenesis - (reply: 5)
328. PCR insert - in frame? - (reply: 2)
329. Whole plasmid amplification by PCR - (reply: 2)
330. colony PCR after transformation - (reply: 1)