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Top : New Forum Archives (2009-): : Molecular-Cloning
301. sequencing problems - (reply: 2)
302. Understand the basics of molecular cloning - (reply: 6)
303. Maintainence of transformed cells - (reply: 5)
304. Restriction digest band shift? - (reply: 1)
305. problem in cloning PCR primer design with restriction site - (reply: 4)
306. quick change mutagenesis...... - (reply: 1)
307. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
308. Help for pGEM-T easy vector ligation problem - (reply: 2)
309. Co-transformation - (reply: 2)
310. Problems regarding point mutations.... - (reply: 4)
311. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
312. pkp 59 vector - (reply: 2)
313. Vector insert ratio in cloning - (reply: 3)
314. polymerase to use for cloning - (reply: 4)
315. Oligo insert cloning - (reply: 3)
316. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
317. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
318. How to select competent E. coli cells? - (reply: 7)
319. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
320. cDNA amplification problem - (reply: 4)
321. Ligation: two basic questions - (reply: 1)
322. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
323. Unexpected base after deletion mutagenesis - (reply: 3)
324. Confirmation of ligation - (reply: 7)
325. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
326. Ligation of large ammount of insert (no transformation) - (reply: 7)
327. PCR and restriction enzyme digestion - (reply: 3)
328. Probelm with double digestion - (reply: 1)
329. Cloning not working, all steps checked, clueless - (reply: 1)
330. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)