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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Plasmid on blotting paper - (reply: 1)
302. Dimerization of PCR product - (reply: 4)
303. How to generate Lentivirus? - (reply: 4)
304. Do genes always need to be cloned in the "right direction"? - (reply: 3)
305. Gel purification (DNA) - (reply: 1)
306. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
307. Cloning and expression problem - (reply: 5)
308. How do silicates adhere nucleic acid? - (reply: 1)
309. Ligation of PCR fragments - (reply: 11)
310. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
311. What plasmid sizes do you work with for SDM? - (reply: 1)
312. cloning help; high salt level in vector - (reply: 4)
313. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
314. problem with gateway cloning: missing part of insert - (reply: 3)
315. T7 and M13 primers two band amplification - (reply: 3)
316. Type of polymerases compatible for TA cloning - (reply: 7)
317. Running undigested plasmids on gel - (reply: 2)
318. Molecular Cloning Sambrook - (reply: 1)
319. What is the membrane made of in DNA purification kits - (reply: 3)
320. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
321. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
322. Removing Introns from a DNA sequence - (reply: 1)
323. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
324. without RE site in PCR product - (reply: 5)
325. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
326. excision of fragment during cloning - (reply: 4)
327. Unusual cloning issue... anyone else?? - (reply: 4)
328. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
329. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
330. How to avoid RNA contamination in DNA samples:? - (reply: 1)