Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
301. Do genes always need to be cloned in the "right direction"? - (reply: 3)
302. Gel purification (DNA) - (reply: 1)
303. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
304. Cloning and expression problem - (reply: 5)
305. How do silicates adhere nucleic acid? - (reply: 1)
306. Ligation of PCR fragments - (reply: 11)
307. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
308. What plasmid sizes do you work with for SDM? - (reply: 1)
309. cloning help; high salt level in vector - (reply: 4)
310. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
311. problem with gateway cloning: missing part of insert - (reply: 3)
312. T7 and M13 primers two band amplification - (reply: 3)
313. Type of polymerases compatible for TA cloning - (reply: 7)
314. Running undigested plasmids on gel - (reply: 2)
315. Molecular Cloning Sambrook - (reply: 1)
316. What is the membrane made of in DNA purification kits - (reply: 3)
317. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
318. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
319. Removing Introns from a DNA sequence - (reply: 1)
320. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
321. without RE site in PCR product - (reply: 5)
322. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
323. excision of fragment during cloning - (reply: 4)
324. Unusual cloning issue... anyone else?? - (reply: 4)
325. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
326. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
327. How to avoid RNA contamination in DNA samples:? - (reply: 1)
328. Wavy Lines in Gel - (reply: 2)
329. Question Relating to LacZ Disruption - (reply: 4)
330. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)