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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
302. Plasmid on blotting paper - (reply: 1)
303. Dimerization of PCR product - (reply: 4)
304. How to generate Lentivirus? - (reply: 4)
305. Do genes always need to be cloned in the "right direction"? - (reply: 3)
306. Gel purification (DNA) - (reply: 1)
307. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
308. Cloning and expression problem - (reply: 5)
309. How do silicates adhere nucleic acid? - (reply: 1)
310. Ligation of PCR fragments - (reply: 11)
311. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
312. What plasmid sizes do you work with for SDM? - (reply: 1)
313. cloning help; high salt level in vector - (reply: 4)
314. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
315. problem with gateway cloning: missing part of insert - (reply: 3)
316. T7 and M13 primers two band amplification - (reply: 3)
317. Type of polymerases compatible for TA cloning - (reply: 7)
318. Running undigested plasmids on gel - (reply: 2)
319. Molecular Cloning Sambrook - (reply: 1)
320. What is the membrane made of in DNA purification kits - (reply: 3)
321. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
322. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
323. Removing Introns from a DNA sequence - (reply: 1)
324. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
325. without RE site in PCR product - (reply: 5)
326. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
327. excision of fragment during cloning - (reply: 4)
328. Unusual cloning issue... anyone else?? - (reply: 4)
329. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
330. Trying to create deletion mutant. Stuck on last step. - (reply: 1)