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Top : New Forum Archives (2009-): : Molecular-Cloning
301. I get band double the size what does it mean - (reply: 3)
302. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
303. Neeed Help , cloning Confirmation ?! - (reply: 5)
304. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
305. Can I quantify restricted plasmid and oligos? - (reply: 3)
306. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
307. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
308. TOPO TA ligation problems - (reply: 6)
309. Luciferase assay problem - (reply: 2)
310. Double digestion with BamHI and BglII - (reply: 4)
311. nicked linear DNA - (reply: 5)
312. Selection of E.coli competent cells - (reply: 2)
313. Double strand cdna synthysis - (reply: 4)
314. mutagenic primers with very high GC content. - (reply: 3)
315. TA Cloning of ds cDNA - (reply: 6)
316. Promoter distance from ATG - (reply: 3)
317. Measuring Fluorescene of RFP - (reply: 7)
318. Smallest possible insert size for Ligation reaction - (reply: 2)
319. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
320. Extraction of DNA from termite gut flagellates - (reply: 7)
321. Enzymes and buffers from different companies - (reply: 6)
322. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
323. faint band after plasmid extraction - (reply: 7)
324. Adaptor ligation - (reply: 9)
325. self ligation - (reply: 1)
326. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
327. Cloning large transgenes - (reply: 2)
328. In-fusion HD cloning - (reply: 3)
329. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
330. Few colonies frm sticky end cloning - (reply: 3)