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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Dimerization of PCR product - (reply: 4)
302. How to generate Lentivirus? - (reply: 4)
303. Do genes always need to be cloned in the "right direction"? - (reply: 3)
304. Gel purification (DNA) - (reply: 1)
305. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
306. Cloning and expression problem - (reply: 5)
307. How do silicates adhere nucleic acid? - (reply: 1)
308. Ligation of PCR fragments - (reply: 11)
309. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
310. What plasmid sizes do you work with for SDM? - (reply: 1)
311. cloning help; high salt level in vector - (reply: 4)
312. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
313. problem with gateway cloning: missing part of insert - (reply: 3)
314. T7 and M13 primers two band amplification - (reply: 3)
315. Type of polymerases compatible for TA cloning - (reply: 7)
316. Running undigested plasmids on gel - (reply: 2)
317. Molecular Cloning Sambrook - (reply: 1)
318. What is the membrane made of in DNA purification kits - (reply: 3)
319. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
320. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
321. Removing Introns from a DNA sequence - (reply: 1)
322. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
323. without RE site in PCR product - (reply: 5)
324. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
325. excision of fragment during cloning - (reply: 4)
326. Unusual cloning issue... anyone else?? - (reply: 4)
327. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
328. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
329. How to avoid RNA contamination in DNA samples:? - (reply: 1)
330. Wavy Lines in Gel - (reply: 2)