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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Ligation of PCR fragments - (reply: 11)
302. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
303. What plasmid sizes do you work with for SDM? - (reply: 1)
304. cloning help; high salt level in vector - (reply: 4)
305. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
306. problem with gateway cloning: missing part of insert - (reply: 3)
307. T7 and M13 primers two band amplification - (reply: 3)
308. Type of polymerases compatible for TA cloning - (reply: 7)
309. Running undigested plasmids on gel - (reply: 2)
310. Molecular Cloning Sambrook - (reply: 1)
311. What is the membrane made of in DNA purification kits - (reply: 3)
312. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
313. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
314. Removing Introns from a DNA sequence - (reply: 1)
315. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
316. without RE site in PCR product - (reply: 5)
317. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
318. excision of fragment during cloning - (reply: 4)
319. Unusual cloning issue... anyone else?? - (reply: 4)
320. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
321. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
322. How to avoid RNA contamination in DNA samples:? - (reply: 1)
323. Wavy Lines in Gel - (reply: 2)
324. Question Relating to LacZ Disruption - (reply: 4)
325. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
326. Site directed mutagenesis - (reply: 5)
327. PCR insert - in frame? - (reply: 2)
328. Whole plasmid amplification by PCR - (reply: 2)
329. colony PCR after transformation - (reply: 1)
330. For promoter assay, which vector I should use? - (reply: 5)