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Top : New Forum Archives (2009-): : Molecular-Cloning
301. competent cells - (reply: 1)
302. "Easiest" sticky end combination - (reply: 3)
303. Cloning advice - (reply: 3)
304. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
305. Cloning large fragments - (reply: 9)
306. Transformation colonies does not contain insert - (reply: 3)
307. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
308. Need urgent advise.. - (reply: 3)
309. Cloning Mitochondrial Targeting Sequence - (reply: 4)
310. problem in cloning - (reply: 2)
311. TA cloning: ligation problem or toxic construct? - (reply: 4)
312. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
313. Do I need to sequence after digestion? - (reply: 9)
314. quick change mutagenesis...... - (reply: 15)
315. Problem with double digestion: one enzyme is not working - (reply: 8)
316. Transient Transfection Controls (Gateway Technology) - (reply: 4)
317. Problems with crossover PCR - (reply: 2)
318. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
319. Two genes, same MCS - (reply: 1)
320. Including ATG in cloning? - (reply: 1)
321. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
322. Low copy vector for library generation - (reply: 2)
323. Can't find a recombinant after ligating - (reply: 4)
324. problems with plasmid miniprep - (reply: 8)
325. gene cloning - (reply: 1)
326. Is ligation really necessary? - (reply: 6)
327. problem with ligation of linker/adaptor - (reply: 2)
328. GFP -weak signal -why? - (reply: 1)
329. Ligation troubleshooting! Please help! - (reply: 5)
330. Truncated gene Cloning - (reply: 1)