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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
302. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
303. How to set up simultaneous digestion? - (reply: 3)
304. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
305. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
306. Freeze transformation mixture? - (reply: 2)
307. Cutting my plasmid made it larger - (reply: 7)
308. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
309. Amplification of CAG promoter problems - (reply: 3)
310. restriction enzyme - (reply: 2)
311. Recreate original plasmid by cutting out insert - (reply: 2)
312. Colony-PCR workaround - (reply: 3)
313. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
314. Can I pause a bacterial culture at 4C - (reply: 2)
315. TOPO TA CLONING - LIGATION STEP - (reply: 9)
316. posting sequences of plasmid? - (reply: 3)
317. Restriction Enzyme Digest not working - (reply: 2)
318. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
319. Incomplete digestion of plasmid with single enzyme - (reply: 1)
320. problem with plasmid digestion - (reply: 5)
321. Difficult Ligation - Details Inside - (reply: 9)
322. pET 32 (+) - (reply: 1)
323. Alternative to pET Vectorsystem - (reply: 2)
324. pEGFP C-1/N-1 Cloning - (reply: 11)
325. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
326. Problems with growing out transformants in liquid LB - (reply: 3)
327. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
328. Ethidiumbromide - (reply: 3)
329. TRYPSIN - (reply: 4)
330. Problem with cloning - what is wrong? - (reply: 6)