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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Scrambled sequence? - (reply: 3)
302. Why is my insert missing from my plasmid? - (reply: 4)
303. PCR Profile for ligation - (reply: 3)
304. Blunt end is ligating to sticky end? - (reply: 2)
305. SNP in the promoter region - (reply: 1)
306. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
307. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
308. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
309. New ordered Restriction Enzymes not working, need help! - (reply: 2)
310. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
311. Transfection but no expression - (reply: 4)
312. Change nucleotide - (reply: 1)
313. Colonies grown without plasmid - (reply: 7)
314. unwanted band - (reply: 5)
315. Colony PCR positive and Digestion negative????? - (reply: 11)
316. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
317. Plasmid on blotting paper - (reply: 1)
318. Dimerization of PCR product - (reply: 4)
319. How to generate Lentivirus? - (reply: 4)
320. Do genes always need to be cloned in the "right direction"? - (reply: 3)
321. Gel purification (DNA) - (reply: 1)
322. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
323. Cloning and expression problem - (reply: 5)
324. How do silicates adhere nucleic acid? - (reply: 1)
325. Ligation of PCR fragments - (reply: 11)
326. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
327. What plasmid sizes do you work with for SDM? - (reply: 1)
328. cloning help; high salt level in vector - (reply: 4)
329. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
330. problem with gateway cloning: missing part of insert - (reply: 3)