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Top : New Forum Archives (2009-): : Molecular-Cloning
301. colony PCR after transformation - (reply: 1)
302. For promoter assay, which vector I should use? - (reply: 5)
303. Sequencing of the cloned plasmid - (reply: 5)
304. 4 way cloning into TA Vector - (reply: 10)
305. I get band double the size what does it mean - (reply: 3)
306. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
307. Neeed Help , cloning Confirmation ?! - (reply: 5)
308. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
309. Can I quantify restricted plasmid and oligos? - (reply: 3)
310. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
311. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
312. TOPO TA ligation problems - (reply: 6)
313. Luciferase assay problem - (reply: 2)
314. Double digestion with BamHI and BglII - (reply: 4)
315. nicked linear DNA - (reply: 5)
316. Selection of E.coli competent cells - (reply: 2)
317. Double strand cdna synthysis - (reply: 4)
318. mutagenic primers with very high GC content. - (reply: 3)
319. TA Cloning of ds cDNA - (reply: 6)
320. Promoter distance from ATG - (reply: 3)
321. Measuring Fluorescene of RFP - (reply: 7)
322. Smallest possible insert size for Ligation reaction - (reply: 2)
323. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
324. Extraction of DNA from termite gut flagellates - (reply: 7)
325. Enzymes and buffers from different companies - (reply: 6)
326. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
327. faint band after plasmid extraction - (reply: 7)
328. Adaptor ligation - (reply: 9)
329. self ligation - (reply: 1)
330. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)