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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Including ATG in cloning? - (reply: 1)
302. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
303. Low copy vector for library generation - (reply: 2)
304. Can't find a recombinant after ligating - (reply: 4)
305. problems with plasmid miniprep - (reply: 8)
306. gene cloning - (reply: 1)
307. Is ligation really necessary? - (reply: 6)
308. problem with ligation of linker/adaptor - (reply: 2)
309. GFP -weak signal -why? - (reply: 1)
310. Ligation troubleshooting! Please help! - (reply: 5)
311. Truncated gene Cloning - (reply: 1)
312. Screening for ligation and transformation result - (reply: 2)
313. Problem with 3 way ligation - (reply: 2)
314. Query regarding primers for quick change mutagenesis - (reply: 3)
315. What is NEB's CutSmart Buffer? - (reply: 5)
316. Enzyme cutting: low efficiency - (reply: 5)
317. Issue with Xba1 and Dam methylation - (reply: 7)
318. Restriction site rearrangement - (reply: 1)
319. Mutation in cloning - (reply: 6)
320. Must the terminator be in frame? - (reply: 1)
321. sequencing problems - (reply: 2)
322. Understand the basics of molecular cloning - (reply: 6)
323. Maintainence of transformed cells - (reply: 5)
324. Restriction digest band shift? - (reply: 1)
325. problem in cloning PCR primer design with restriction site - (reply: 4)
326. quick change mutagenesis...... - (reply: 1)
327. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
328. Help for pGEM-T easy vector ligation problem - (reply: 2)
329. Co-transformation - (reply: 2)
330. Problems regarding point mutations.... - (reply: 4)