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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Help~ Incorrect constructs in cloning - (reply: 3)
302. Problems with ligation and transformation - (reply: 2)
303. Transformation of Restricted plasmid - (reply: 3)
304. Scrambled sequence? - (reply: 3)
305. Why is my insert missing from my plasmid? - (reply: 4)
306. PCR Profile for ligation - (reply: 3)
307. Blunt end is ligating to sticky end? - (reply: 2)
308. SNP in the promoter region - (reply: 1)
309. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
310. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
311. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
312. New ordered Restriction Enzymes not working, need help! - (reply: 2)
313. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
314. Transfection but no expression - (reply: 4)
315. Change nucleotide - (reply: 1)
316. Colonies grown without plasmid - (reply: 7)
317. unwanted band - (reply: 5)
318. Colony PCR positive and Digestion negative????? - (reply: 11)
319. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
320. Plasmid on blotting paper - (reply: 1)
321. Dimerization of PCR product - (reply: 4)
322. How to generate Lentivirus? - (reply: 4)
323. Do genes always need to be cloned in the "right direction"? - (reply: 3)
324. Gel purification (DNA) - (reply: 1)
325. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
326. Cloning and expression problem - (reply: 5)
327. How do silicates adhere nucleic acid? - (reply: 1)
328. Ligation of PCR fragments - (reply: 11)
329. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
330. What plasmid sizes do you work with for SDM? - (reply: 1)