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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Recovery of competence of BH10 bacteria - is it possible? - (reply: 2)
302. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
303. New ordered Restriction Enzymes not working, need help! - (reply: 2)
304. Will a Lac promoter sequence interrupt transcription? - (reply: 6)
305. Transfection but no expression - (reply: 4)
306. Change nucleotide - (reply: 1)
307. Colonies grown without plasmid - (reply: 7)
308. unwanted band - (reply: 5)
309. Colony PCR positive and Digestion negative????? - (reply: 11)
310. Seperating sequences with single base pair mutations from a cDNA library - (reply: 1)
311. Plasmid on blotting paper - (reply: 1)
312. Dimerization of PCR product - (reply: 4)
313. How to generate Lentivirus? - (reply: 4)
314. Do genes always need to be cloned in the "right direction"? - (reply: 3)
315. Gel purification (DNA) - (reply: 1)
316. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
317. Cloning and expression problem - (reply: 5)
318. How do silicates adhere nucleic acid? - (reply: 1)
319. Ligation of PCR fragments - (reply: 11)
320. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
321. What plasmid sizes do you work with for SDM? - (reply: 1)
322. cloning help; high salt level in vector - (reply: 4)
323. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
324. problem with gateway cloning: missing part of insert - (reply: 3)
325. T7 and M13 primers two band amplification - (reply: 3)
326. Type of polymerases compatible for TA cloning - (reply: 7)
327. Running undigested plasmids on gel - (reply: 2)
328. Molecular Cloning Sambrook - (reply: 1)
329. What is the membrane made of in DNA purification kits - (reply: 3)
330. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)