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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Double strand cdna synthysis - (reply: 4)
302. mutagenic primers with very high GC content. - (reply: 3)
303. TA Cloning of ds cDNA - (reply: 6)
304. Promoter distance from ATG - (reply: 3)
305. Measuring Fluorescene of RFP - (reply: 7)
306. Smallest possible insert size for Ligation reaction - (reply: 2)
307. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
308. Extraction of DNA from termite gut flagellates - (reply: 7)
309. Enzymes and buffers from different companies - (reply: 6)
310. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
311. faint band after plasmid extraction - (reply: 7)
312. Adaptor ligation - (reply: 9)
313. self ligation - (reply: 1)
314. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
315. Cloning large transgenes - (reply: 2)
316. In-fusion HD cloning - (reply: 3)
317. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
318. Few colonies frm sticky end cloning - (reply: 3)
319. transformation problem - (reply: 8)
320. Problems with Digestion? - (reply: 7)
321. plasmid digestion - problem with enzymes? - (reply: 4)
322. Problem with cloning - (reply: 7)
323. double transformation problem - (reply: 3)
324. Too many negative clones using Directional TOPO Cloning - (reply: 5)
325. competent cells - (reply: 1)
326. "Easiest" sticky end combination - (reply: 3)
327. Cloning advice - (reply: 3)
328. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
329. Cloning large fragments - (reply: 9)
330. Transformation colonies does not contain insert - (reply: 3)