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Top : New Forum Archives (2009-): : Molecular-Cloning
301. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
302. without RE site in PCR product - (reply: 5)
303. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
304. excision of fragment during cloning - (reply: 4)
305. Unusual cloning issue... anyone else?? - (reply: 4)
306. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
307. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
308. How to avoid RNA contamination in DNA samples:? - (reply: 1)
309. Wavy Lines in Gel - (reply: 2)
310. Question Relating to LacZ Disruption - (reply: 4)
311. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
312. Site directed mutagenesis - (reply: 5)
313. PCR insert - in frame? - (reply: 2)
314. Whole plasmid amplification by PCR - (reply: 2)
315. colony PCR after transformation - (reply: 1)
316. For promoter assay, which vector I should use? - (reply: 5)
317. Sequencing of the cloned plasmid - (reply: 5)
318. 4 way cloning into TA Vector - (reply: 10)
319. I get band double the size what does it mean - (reply: 3)
320. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
321. Neeed Help , cloning Confirmation ?! - (reply: 5)
322. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
323. Can I quantify restricted plasmid and oligos? - (reply: 3)
324. Pseudomonas aeruginosa 14 with pUC19? - (reply: 2)
325. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
326. TOPO TA ligation problems - (reply: 6)
327. Luciferase assay problem - (reply: 2)
328. Double digestion with BamHI and BglII - (reply: 4)
329. nicked linear DNA - (reply: 5)
330. Selection of E.coli competent cells - (reply: 2)