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Top : New Forum Archives (2009-): : Molecular-Cloning
301. No Colonies after Electroporation of P.aeruginosa - (reply: 6)
302. What plasmid sizes do you work with for SDM? - (reply: 1)
303. cloning help; high salt level in vector - (reply: 4)
304. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
305. problem with gateway cloning: missing part of insert - (reply: 3)
306. T7 and M13 primers two band amplification - (reply: 3)
307. Type of polymerases compatible for TA cloning - (reply: 7)
308. Running undigested plasmids on gel - (reply: 2)
309. Molecular Cloning Sambrook - (reply: 1)
310. What is the membrane made of in DNA purification kits - (reply: 3)
311. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
312. Can you engineer a kinetochore in a BAC so that it segregates evenly - (reply: 3)
313. Removing Introns from a DNA sequence - (reply: 1)
314. transfecting Jurkat with Fugene HD: does not work? - (reply: 4)
315. without RE site in PCR product - (reply: 5)
316. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
317. excision of fragment during cloning - (reply: 4)
318. Unusual cloning issue... anyone else?? - (reply: 4)
319. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
320. Trying to create deletion mutant. Stuck on last step. - (reply: 1)
321. How to avoid RNA contamination in DNA samples:? - (reply: 1)
322. Wavy Lines in Gel - (reply: 2)
323. Question Relating to LacZ Disruption - (reply: 4)
324. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
325. Site directed mutagenesis - (reply: 5)
326. PCR insert - in frame? - (reply: 2)
327. Whole plasmid amplification by PCR - (reply: 2)
328. colony PCR after transformation - (reply: 1)
329. For promoter assay, which vector I should use? - (reply: 5)
330. Sequencing of the cloned plasmid - (reply: 5)