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Top : New Forum Archives (2009-): : Molecular-Cloning
301. polymerase to use for cloning - (reply: 4)
302. Oligo insert cloning - (reply: 3)
303. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
304. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
305. How to select competent E. coli cells? - (reply: 7)
306. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
307. cDNA amplification problem - (reply: 4)
308. Ligation: two basic questions - (reply: 1)
309. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
310. Unexpected base after deletion mutagenesis - (reply: 3)
311. Confirmation of ligation - (reply: 7)
312. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
313. Ligation of large ammount of insert (no transformation) - (reply: 7)
314. PCR and restriction enzyme digestion - (reply: 3)
315. Probelm with double digestion - (reply: 1)
316. Cloning not working, all steps checked, clueless - (reply: 1)
317. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
318. recombinant protein form - (reply: 2)
319. restricted PCR plasmid runs slower - (reply: 2)
320. Cloning problem, sticky - blunt ends ligation fail . - (reply: 4)
321. High frequency of vector religation - (reply: 10)
322. Cloned but not expressing! - (reply: 8)
323. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
324. DNA ligation and trasformation - (reply: 6)
325. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
326. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
327. How to set up simultaneous digestion? - (reply: 3)
328. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
329. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
330. Freeze transformation mixture? - (reply: 2)