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Top : New Forum Archives (2009-): : Molecular-Cloning
301. Restriction site rearrangement - (reply: 1)
302. Mutation in cloning - (reply: 6)
303. Must the terminator be in frame? - (reply: 1)
304. sequencing problems - (reply: 2)
305. Understand the basics of molecular cloning - (reply: 6)
306. Maintainence of transformed cells - (reply: 5)
307. Restriction digest band shift? - (reply: 1)
308. problem in cloning PCR primer design with restriction site - (reply: 4)
309. quick change mutagenesis...... - (reply: 1)
310. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
311. Help for pGEM-T easy vector ligation problem - (reply: 2)
312. Co-transformation - (reply: 2)
313. Problems regarding point mutations.... - (reply: 4)
314. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
315. pkp 59 vector - (reply: 2)
316. Vector insert ratio in cloning - (reply: 3)
317. polymerase to use for cloning - (reply: 4)
318. Oligo insert cloning - (reply: 3)
319. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
320. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
321. How to select competent E. coli cells? - (reply: 7)
322. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
323. cDNA amplification problem - (reply: 4)
324. Ligation: two basic questions - (reply: 1)
325. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
326. Unexpected base after deletion mutagenesis - (reply: 3)
327. Confirmation of ligation - (reply: 7)
328. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
329. Ligation of large ammount of insert (no transformation) - (reply: 7)
330. PCR and restriction enzyme digestion - (reply: 3)