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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Radioactive carbon - (reply: 3)
1052. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1053. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1054. Digesting large amount of plasmid - (reply: 4)
1055. cleanup of digestion before ligation - (reply: 6)
1056. transformation efficiency - (reply: 7)
1057. transformed bacteria doesnt grow - (reply: 1)
1058. TOPO Cloning - (reply: 5)
1059. blunt ligation - (reply: 4)
1060. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1061. About Laminin 5'UTR 's function - (reply: 1)
1062. Vectors with special MCS - (reply: 5)
1063. Cloning <100 bp fragments into a vector - (reply: 7)
1064. Help! my plasmid size changed after ligation - (reply: 5)
1065. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1066. Restriction digestion of gDNA for phage library - (reply: 5)
1067. how i can get insert back? - (reply: 1)
1068. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1069. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1070. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1071. mammalian expression vector - (reply: 2)
1072. Electroporation doesn't like my plasmid??? - (reply: 4)
1073. low yeild fo RNA after invitro transcription - (reply: 4)
1074. No expresion of transgene - Retroviral vector (reply: 1)
1075. plasmids - plasmids for stable cell line construction (reply: 2)
1076. empty vectors more than the insert - (reply: 6)
1077. Restriction enzyme digestion problem - (reply: 2)
1078. low copy plasmid - how to improve the yield? (reply: 1)
1079. How to anneal more than 2 oligos???? - Protocol wanted that allows the annealing of several oligos (reply: 2)
1080. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)