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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1052. Red/ET recombination - (reply: 2)
1053. multiple origins of replication - (reply: 2)
1054. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1055. Single Restriction Site cloning - Cloning (reply: 6)
1056. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1057. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1058. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1059. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1060. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1061. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1062. Agarose gel electrophoresis question - (reply: 1)
1063. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1064. My white colonies turned blue in frig! Why? - (reply: 6)
1065. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1066. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1067. Dephosphorylation of vector - (reply: 8)
1068. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1069. rna isolation - (reply: 1)
1070. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1071. So many colonies! - (reply: 8)
1072. Large vector cloning-recombination problem? - (reply: 1)
1073. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1074. selecting recombinants - (reply: 1)
1075. colony PCR for subcloning gene - (reply: 1)
1076. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1077. cloning of a 20 kb insert - (reply: 2)
1078. DNA quantification after gel purification - (reply: 6)
1079. cloning of an operon - (reply: 2)
1080. storing plasmid - -80degrees (reply: 4)