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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Double digestion and ligation problem - (reply: 1)
1052. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1053. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1054. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1055. CMV promoter - (reply: 2)
1056. insert in the reverse orientation after transformation - (reply: 5)
1057. cDNA as PCR template - (reply: 1)
1058. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1059. T4 ligase buffer - (reply: 4)
1060. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1061. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1062. ligation problems - no colonies at the end!!!!! (reply: 4)
1063. Good stable mammalian promoter - (reply: 1)
1064. Self-ligation from a Double Digest - (reply: 4)
1065. Strange frameshift question - (reply: 2)
1066. Plasmid DNA mini-prep - no good isolation (reply: 5)
1067. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1068. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1069. BstUI digestion - (reply: 1)
1070. I have cloning problems with pGEM-T Easy - (reply: 5)
1071. CaCl2 for making competent cells - (reply: 3)
1072. best way to transport bacteria at room temperature - (reply: 5)
1073. Suicide vector - (reply: 2)
1074. Extremely small bacterial colonies after transformation - (reply: 10)
1075. tranformation efficiency - (reply: 2)
1076. transfection time - (reply: 2)
1077. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1078. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1079. Problem with SmaI restriction digest - (reply: 6)
1080. blunt-end / 3ŽA overhang cloning - (reply: 1)