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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Large DNA extraction from an agarose gel - (reply: 8)
1052. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1053. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1054. Ligation problem, religation of backbone without any insert? - (reply: 3)
1055. problem with pLK01 plasmid ligation - (reply: 2)
1056. Mini prep streaking - basic question (reply: 3)
1057. In-fusion joining few parts together - possible? ideas? (reply: 3)
1058. TOPO cloning problem - (reply: 4)
1059. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1060. Red/ET recombination - (reply: 2)
1061. multiple origins of replication - (reply: 2)
1062. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1063. Single Restriction Site cloning - Cloning (reply: 6)
1064. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1065. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1066. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1067. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1068. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1069. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1070. Agarose gel electrophoresis question - (reply: 1)
1071. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1072. My white colonies turned blue in frig! Why? - (reply: 6)
1073. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1074. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1075. Dephosphorylation of vector - (reply: 8)
1076. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1077. rna isolation - (reply: 1)
1078. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1079. So many colonies! - (reply: 8)
1080. Large vector cloning-recombination problem? - (reply: 1)