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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1052. My white colonies turned blue in frig! Why? - (reply: 6)
1053. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1054. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1055. Dephosphorylation of vector - (reply: 8)
1056. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1057. rna isolation - (reply: 1)
1058. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1059. So many colonies! - (reply: 8)
1060. Large vector cloning-recombination problem? - (reply: 1)
1061. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1062. selecting recombinants - (reply: 1)
1063. colony PCR for subcloning gene - (reply: 1)
1064. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1065. cloning of a 20 kb insert - (reply: 2)
1066. DNA quantification after gel purification - (reply: 6)
1067. cloning of an operon - (reply: 2)
1068. storing plasmid - -80degrees (reply: 4)
1069. verifying a positive transformant - (reply: 3)
1070. Need advice on cloning very small insert into a vector - (reply: 3)
1071. Cloning problems i.e. Ligation - (reply: 2)
1072. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1073. Radioactive carbon - (reply: 3)
1074. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1075. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1076. Digesting large amount of plasmid - (reply: 4)
1077. cleanup of digestion before ligation - (reply: 6)
1078. transformation efficiency - (reply: 7)
1079. transformed bacteria doesnt grow - (reply: 1)
1080. TOPO Cloning - (reply: 5)