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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Plasmid DNA mini-prep - no good isolation (reply: 5)
1052. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1053. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
1054. BstUI digestion - (reply: 1)
1055. I have cloning problems with pGEM-T Easy - (reply: 5)
1056. CaCl2 for making competent cells - (reply: 3)
1057. best way to transport bacteria at room temperature - (reply: 5)
1058. Suicide vector - (reply: 2)
1059. Extremely small bacterial colonies after transformation - (reply: 10)
1060. tranformation efficiency - (reply: 2)
1061. transfection time - (reply: 2)
1062. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
1063. T4 buffer freeze thaw - can we use the same buffer if thawed before? (reply: 2)
1064. Problem with SmaI restriction digest - (reply: 6)
1065. blunt-end / 3ŽA overhang cloning - (reply: 1)
1066. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
1067. overexpression of stable clones - (reply: 1)
1068. functionality of yeast promoter in E. coli - (reply: 1)
1069. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
1070. Can't seem to do a simple cloning - (reply: 2)
1071. Histogram from Nanodrop - (reply: 1)
1072. cDNA library construction - I get only one colony (reply: 2)
1073. Cloning into pIBV5-His (not working) - (reply: 1)
1074. Addition of Restriction sites into PCR primers - (reply: 4)
1075. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
1076. Streaky DNA digest - (reply: 3)
1077. TOPO TA cloning problem - (reply: 12)
1078. cloning problem - (reply: 2)
1079. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
1080. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)