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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Cloning problems - (reply: 7)
1052. Bacteria and Amaxa - (reply: 2)
1053. Problem in Ligation - (reply: 12)
1054. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1055. multiway ligation - (reply: 6)
1056. Plasmid size problems! - (reply: 3)
1057. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1058. ligation problem - (reply: 6)
1059. Failed Ligation - (reply: 2)
1060. cloning of GroEL - (reply: 3)
1061. Ligation problem - (reply: 9)
1062. Problems with High Background with Gateway Cloning - (reply: 1)
1063. Mutagenesis troubles - (reply: 2)
1064. ligation/transformation failed - (reply: 7)
1065. Transfecting primary cells with plasmid - (reply: 1)
1066. Extra bands after QIAGEN spin miniprep - (reply: 3)
1067. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1068. The use of hygromycin - (reply: 2)
1069. QUESTION about cloning - (reply: 5)
1070. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1071. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1072. Ligation screening - (reply: 4)
1073. Double digestion and ligation problem - (reply: 1)
1074. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1075. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1076. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1077. CMV promoter - (reply: 2)
1078. insert in the reverse orientation after transformation - (reply: 5)
1079. cDNA as PCR template - (reply: 1)
1080. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)