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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
1052. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
1053. Stable Mammalian Expression with Retrovirus - (reply: 2)
1054. Neon Transfection System - (reply: 2)
1055. help - (reply: 5)
1056. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
1057. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
1058. problems with qiagen gel extraction kit - (reply: 7)
1059. restriction enzyme flanking sequence preferance - (reply: 2)
1060. pCDH Lentivector has 500 bp insert - (reply: 5)
1061. 5' RACE for very GC-rich gene - (reply: 2)
1062. Phosphorylated primers - (reply: 5)
1063. Correct orientation of the insert - (reply: 7)
1064. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
1065. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
1066. Cloning advice needed - (reply: 4)
1067. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
1068. Colony Screeing without using PCR - (reply: 8)
1069. blue white screening - (reply: 3)
1070. phosphorylate dprimers for pfx do not work - (reply: 3)
1071. help - (reply: 1)
1072. problem with yeast transformation - (reply: 2)
1073. ta cloning of pfu amplified fragment - (reply: 9)
1074. how to express the gene in opposite transcriptional direction - (reply: 3)
1075. Water for electrocompetent cells - (reply: 2)
1076. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
1077. Bacterial growth in agar plate with ampicillin - (reply: 4)
1078. problem in ligation and cloning - (reply: 1)
1079. Electrophoresis gel boxes and geldoc system - (reply: 2)
1080. ampicillin concentration trouble - What is the highest concentration possible to use? (reply: 2)