Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Ligation problem - (reply: 9)
1052. Problems with High Background with Gateway Cloning - (reply: 1)
1053. Mutagenesis troubles - (reply: 2)
1054. ligation/transformation failed - (reply: 7)
1055. Transfecting primary cells with plasmid - (reply: 1)
1056. Extra bands after QIAGEN spin miniprep - (reply: 3)
1057. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
1058. The use of hygromycin - (reply: 2)
1059. QUESTION about cloning - (reply: 5)
1060. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
1061. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
1062. Ligation screening - (reply: 4)
1063. Double digestion and ligation problem - (reply: 1)
1064. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
1065. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
1066. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
1067. CMV promoter - (reply: 2)
1068. insert in the reverse orientation after transformation - (reply: 5)
1069. cDNA as PCR template - (reply: 1)
1070. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
1071. T4 ligase buffer - (reply: 4)
1072. Kanamaycin bugs grow on Chloramphenicol plates... - (reply: 2)
1073. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
1074. ligation problems - no colonies at the end!!!!! (reply: 4)
1075. Good stable mammalian promoter - (reply: 1)
1076. Self-ligation from a Double Digest - (reply: 4)
1077. Strange frameshift question - (reply: 2)
1078. Plasmid DNA mini-prep - no good isolation (reply: 5)
1079. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
1080. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)