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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1052. Dephosphorylation of vector - (reply: 8)
1053. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1054. rna isolation - (reply: 1)
1055. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1056. So many colonies! - (reply: 8)
1057. Large vector cloning-recombination problem? - (reply: 1)
1058. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1059. selecting recombinants - (reply: 1)
1060. colony PCR for subcloning gene - (reply: 1)
1061. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1062. cloning of a 20 kb insert - (reply: 2)
1063. DNA quantification after gel purification - (reply: 6)
1064. cloning of an operon - (reply: 2)
1065. storing plasmid - -80degrees (reply: 4)
1066. verifying a positive transformant - (reply: 3)
1067. Need advice on cloning very small insert into a vector - (reply: 3)
1068. Cloning problems i.e. Ligation - (reply: 2)
1069. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1070. Radioactive carbon - (reply: 3)
1071. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1072. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1073. Digesting large amount of plasmid - (reply: 4)
1074. cleanup of digestion before ligation - (reply: 6)
1075. transformation efficiency - (reply: 7)
1076. transformed bacteria doesnt grow - (reply: 1)
1077. TOPO Cloning - (reply: 5)
1078. blunt ligation - (reply: 4)
1079. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1080. About Laminin 5'UTR 's function - (reply: 1)