Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
1051. multiple origins of replication - (reply: 2)
1052. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1053. Single Restriction Site cloning - Cloning (reply: 6)
1054. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1055. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1056. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1057. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1058. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1059. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1060. Agarose gel electrophoresis question - (reply: 1)
1061. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1062. My white colonies turned blue in frig! Why? - (reply: 6)
1063. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1064. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1065. Dephosphorylation of vector - (reply: 8)
1066. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1067. rna isolation - (reply: 1)
1068. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1069. So many colonies! - (reply: 8)
1070. Large vector cloning-recombination problem? - (reply: 1)
1071. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1072. selecting recombinants - (reply: 1)
1073. colony PCR for subcloning gene - (reply: 1)
1074. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1075. cloning of a 20 kb insert - (reply: 2)
1076. DNA quantification after gel purification - (reply: 6)
1077. cloning of an operon - (reply: 2)
1078. storing plasmid - -80degrees (reply: 4)
1079. verifying a positive transformant - (reply: 3)
1080. Need advice on cloning very small insert into a vector - (reply: 3)