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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. storing plasmid - -80degrees (reply: 4)
1052. verifying a positive transformant - (reply: 3)
1053. Need advice on cloning very small insert into a vector - (reply: 3)
1054. Cloning problems i.e. Ligation - (reply: 2)
1055. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1056. Radioactive carbon - (reply: 3)
1057. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1058. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1059. Digesting large amount of plasmid - (reply: 4)
1060. cleanup of digestion before ligation - (reply: 6)
1061. transformation efficiency - (reply: 7)
1062. transformed bacteria doesnt grow - (reply: 1)
1063. TOPO Cloning - (reply: 5)
1064. blunt ligation - (reply: 4)
1065. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1066. About Laminin 5'UTR 's function - (reply: 1)
1067. Vectors with special MCS - (reply: 5)
1068. Cloning <100 bp fragments into a vector - (reply: 7)
1069. Help! my plasmid size changed after ligation - (reply: 5)
1070. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
1071. Restriction digestion of gDNA for phage library - (reply: 5)
1072. how i can get insert back? - (reply: 1)
1073. DNA precipitation- Why EtOH? - Why we use EtOH and NaOAc together? (reply: 1)
1074. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
1075. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
1076. mammalian expression vector - (reply: 2)
1077. Electroporation doesn't like my plasmid??? - (reply: 4)
1078. low yeild fo RNA after invitro transcription - (reply: 4)
1079. No expresion of transgene - Retroviral vector (reply: 1)
1080. plasmids - plasmids for stable cell line construction (reply: 2)