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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
1052. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
1053. Cloning Help!! - It is getting very annoying... (reply: 13)
1054. restriction digestion - (reply: 7)
1055. sequence errors created during cloning - (reply: 6)
1056. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
1057. the pyrithiamine - (reply: 5)
1058. Please advise on my transgene strategy :) - Thanks! (reply: 4)
1059. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
1060. What is the role of DMSO in yeast transformation - (reply: 1)
1061. ligation problem - (reply: 3)
1062. After Transfection - (reply: 1)
1063. pGEMT easy TA cloning - does electroporation work? (reply: 1)
1064. Help cloning large insert into large vector - (reply: 7)
1065. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1066. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1067. hENT1 - (reply: 1)
1068. digestion of insert twice and ligation of 3 bits - (reply: 6)
1069. blunt end ligation problem - Self ligation and truncate (reply: 1)
1070. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1071. neb ligase - (reply: 2)
1072. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1073. problem about vector Dephosphorylation - (reply: 2)
1074. Early transfection - (reply: 6)
1075. Cloning: Randomly recombined Plasmids? - (reply: 6)
1076. problem about fused protein - problem about fused protein (reply: 2)
1077. Bacteria growing on plate but not liquid media - (reply: 8)
1078. What means BamHI-linkered - (reply: 1)
1079. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1080. Problems with Dpn1 - (reply: 5)