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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. Agarose gel electrophoresis question - (reply: 1)
1052. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1053. My white colonies turned blue in frig! Why? - (reply: 6)
1054. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1055. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1056. Dephosphorylation of vector - (reply: 8)
1057. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1058. rna isolation - (reply: 1)
1059. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1060. So many colonies! - (reply: 8)
1061. Large vector cloning-recombination problem? - (reply: 1)
1062. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1063. selecting recombinants - (reply: 1)
1064. colony PCR for subcloning gene - (reply: 1)
1065. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1066. cloning of a 20 kb insert - (reply: 2)
1067. DNA quantification after gel purification - (reply: 6)
1068. cloning of an operon - (reply: 2)
1069. storing plasmid - -80degrees (reply: 4)
1070. verifying a positive transformant - (reply: 3)
1071. Need advice on cloning very small insert into a vector - (reply: 3)
1072. Cloning problems i.e. Ligation - (reply: 2)
1073. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1074. Radioactive carbon - (reply: 3)
1075. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1076. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1077. Digesting large amount of plasmid - (reply: 4)
1078. cleanup of digestion before ligation - (reply: 6)
1079. transformation efficiency - (reply: 7)
1080. transformed bacteria doesnt grow - (reply: 1)