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Top : New Forum Archives (2009-): : Molecular-Cloning
1051. My white colonies turned blue in frig! Why? - (reply: 6)
1052. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1053. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1054. Dephosphorylation of vector - (reply: 8)
1055. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1056. rna isolation - (reply: 1)
1057. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1058. So many colonies! - (reply: 8)
1059. Large vector cloning-recombination problem? - (reply: 1)
1060. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1061. selecting recombinants - (reply: 1)
1062. colony PCR for subcloning gene - (reply: 1)
1063. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1064. cloning of a 20 kb insert - (reply: 2)
1065. DNA quantification after gel purification - (reply: 6)
1066. cloning of an operon - (reply: 2)
1067. storing plasmid - -80degrees (reply: 4)
1068. verifying a positive transformant - (reply: 3)
1069. Need advice on cloning very small insert into a vector - (reply: 3)
1070. Cloning problems i.e. Ligation - (reply: 2)
1071. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1072. Radioactive carbon - (reply: 3)
1073. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1074. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1075. Digesting large amount of plasmid - (reply: 4)
1076. cleanup of digestion before ligation - (reply: 6)
1077. transformation efficiency - (reply: 7)
1078. transformed bacteria doesnt grow - (reply: 1)
1079. TOPO Cloning - (reply: 5)
1080. blunt ligation - (reply: 4)