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841. Chemical competent E.coli always from single colony ? - (reply: 2)
842. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
843. Strange results of cloning - insert always reverse-oriented (reply: 2)
844. electroporation - (reply: 2)
845. transfection or infection - difference? (reply: 1)
846. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
847. No colonies after transformation! - (reply: 2)
848. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
849. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
850. Help Miniprep kit SIGMA - (reply: 2)
851. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
852. A question about pBR322 and pUC ori - (reply: 3)
853. Digestion of mini-prep by mistake - (reply: 3)
854. Cloning issue - (reply: 3)
855. Cohesive end filling after CIAP treatment - (reply: 2)
856. Looking for Pfu Taq for high GC-content template - (reply: 7)
857. questions about transformation - (reply: 4)
858. genotype lysogen DE3 - (reply: 1)
859. Need help! - Problem with right insertion! (reply: 1)
860. cloning a large insert - (reply: 5)
861. Transposase contamination in cloning - (reply: 7)
862. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
863. How long can I store cleaved/digested vector? - (reply: 9)
864. Cloning of a gene containing 31 exon - (reply: 1)
865. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
866. Optimum Molar ratio for 3-way ligation - (reply: 5)
867. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
868. cloning help - (reply: 3)
869. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
870. A gene is not inserted into the Plasmid - (reply: 2)
842. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
843. Strange results of cloning - insert always reverse-oriented (reply: 2)
844. electroporation - (reply: 2)
845. transfection or infection - difference? (reply: 1)
846. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
847. No colonies after transformation! - (reply: 2)
848. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
849. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
850. Help Miniprep kit SIGMA - (reply: 2)
851. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
852. A question about pBR322 and pUC ori - (reply: 3)
853. Digestion of mini-prep by mistake - (reply: 3)
854. Cloning issue - (reply: 3)
855. Cohesive end filling after CIAP treatment - (reply: 2)
856. Looking for Pfu Taq for high GC-content template - (reply: 7)
857. questions about transformation - (reply: 4)
858. genotype lysogen DE3 - (reply: 1)
859. Need help! - Problem with right insertion! (reply: 1)
860. cloning a large insert - (reply: 5)
861. Transposase contamination in cloning - (reply: 7)
862. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
863. How long can I store cleaved/digested vector? - (reply: 9)
864. Cloning of a gene containing 31 exon - (reply: 1)
865. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
866. Optimum Molar ratio for 3-way ligation - (reply: 5)
867. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
868. cloning help - (reply: 3)
869. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
870. A gene is not inserted into the Plasmid - (reply: 2)