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841. cloning failure help plzzz - cloning (reply: 1)
842. use of pGEM -tT easy vectors - (reply: 2)
843. ~200kb Plasmid DNA purification is not working - (reply: 1)
844. ligating a 6 His tag to plasmid - (reply: 2)
845. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
846. ligation and transformation problem - (reply: 1)
847. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
848. Mini prep of frozen bacteria cells - (reply: 2)
849. PCR cloning problem - could not get colony (reply: 4)
850. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
851. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
852. PCR clonning - (reply: 3)
853. 2 bands in blue colony PCR - (reply: 5)
854. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
855. Introduction to vector development - Basics on vectors types and construction (reply: 1)
856. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
857. Targeted mutagenesis using a linear fragment - (reply: 1)
858. Transformation & Minimal Plates - (reply: 5)
859. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
860. Subcloning Problem - (reply: 3)
861. Restriction Enzyme sites in my genes - (reply: 1)
862. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
863. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
864. In-Fusion (Clontech) Reaction buffer - (reply: 1)
865. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
866. MDA-MB-231 cell transfection - (reply: 5)
867. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
868. Break Cell Clumps - prepare single cells for injections (reply: 1)
869. 126pb insert clonnig/ digestion issues - (reply: 3)
870. cloning problem... - (reply: 5)
842. use of pGEM -tT easy vectors - (reply: 2)
843. ~200kb Plasmid DNA purification is not working - (reply: 1)
844. ligating a 6 His tag to plasmid - (reply: 2)
845. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
846. ligation and transformation problem - (reply: 1)
847. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
848. Mini prep of frozen bacteria cells - (reply: 2)
849. PCR cloning problem - could not get colony (reply: 4)
850. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
851. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
852. PCR clonning - (reply: 3)
853. 2 bands in blue colony PCR - (reply: 5)
854. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
855. Introduction to vector development - Basics on vectors types and construction (reply: 1)
856. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
857. Targeted mutagenesis using a linear fragment - (reply: 1)
858. Transformation & Minimal Plates - (reply: 5)
859. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
860. Subcloning Problem - (reply: 3)
861. Restriction Enzyme sites in my genes - (reply: 1)
862. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
863. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
864. In-Fusion (Clontech) Reaction buffer - (reply: 1)
865. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
866. MDA-MB-231 cell transfection - (reply: 5)
867. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
868. Break Cell Clumps - prepare single cells for injections (reply: 1)
869. 126pb insert clonnig/ digestion issues - (reply: 3)
870. cloning problem... - (reply: 5)