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Top : New Forum Archives (2009-): : Molecular-Cloning
841. PCR clonning - (reply: 3)
842. 2 bands in blue colony PCR - (reply: 5)
843. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
844. Introduction to vector development - Basics on vectors types and construction (reply: 1)
845. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
846. Targeted mutagenesis using a linear fragment - (reply: 1)
847. Transformation & Minimal Plates - (reply: 5)
848. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
849. Subcloning Problem - (reply: 3)
850. Restriction Enzyme sites in my genes - (reply: 1)
851. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
852. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
853. In-Fusion (Clontech) Reaction buffer - (reply: 1)
854. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
855. MDA-MB-231 cell transfection - (reply: 5)
856. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
857. Break Cell Clumps - prepare single cells for injections (reply: 1)
858. 126pb insert clonnig/ digestion issues - (reply: 3)
859. cloning problem... - (reply: 5)
860. yeast glycerol stock - (reply: 2)
861. yeast transformation - (reply: 1)
862. Problems with ligation and transformation - (reply: 2)
863. very small insert and a very ling vector - (reply: 1)
864. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
865. cDNA clone - (reply: 1)
866. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
867. why these guys use T7 instead of CMV? - for transfection (reply: 2)
868. Electroporation - no colonies (reply: 1)
869. enzymes inactivation - (reply: 3)
870. help: why plasmid yield dropped ever since the first culture - (reply: 2)