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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Targeted mutagenesis using a linear fragment - (reply: 1)
842. Transformation & Minimal Plates - (reply: 5)
843. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
844. Subcloning Problem - (reply: 3)
845. Restriction Enzyme sites in my genes - (reply: 1)
846. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
847. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
848. In-Fusion (Clontech) Reaction buffer - (reply: 1)
849. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
850. MDA-MB-231 cell transfection - (reply: 5)
851. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
852. Break Cell Clumps - prepare single cells for injections (reply: 1)
853. 126pb insert clonnig/ digestion issues - (reply: 3)
854. cloning problem... - (reply: 5)
855. yeast glycerol stock - (reply: 2)
856. yeast transformation - (reply: 1)
857. Problems with ligation and transformation - (reply: 2)
858. very small insert and a very ling vector - (reply: 1)
859. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
860. cDNA clone - (reply: 1)
861. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
862. why these guys use T7 instead of CMV? - for transfection (reply: 2)
863. Electroporation - no colonies (reply: 1)
864. enzymes inactivation - (reply: 3)
865. help: why plasmid yield dropped ever since the first culture - (reply: 2)
866. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
867. miRNA cloning - (reply: 2)
868. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
869. Single-digested vector shifts downward on gel - (reply: 1)
870. problem in digestion - (reply: 1)