Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
841. use of pGEM -tT easy vectors - (reply: 2)
842. ~200kb Plasmid DNA purification is not working - (reply: 1)
843. ligating a 6 His tag to plasmid - (reply: 2)
844. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
845. ligation and transformation problem - (reply: 1)
846. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
847. Mini prep of frozen bacteria cells - (reply: 2)
848. PCR cloning problem - could not get colony (reply: 4)
849. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
850. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
851. PCR clonning - (reply: 3)
852. 2 bands in blue colony PCR - (reply: 5)
853. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
854. Introduction to vector development - Basics on vectors types and construction (reply: 1)
855. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
856. Targeted mutagenesis using a linear fragment - (reply: 1)
857. Transformation & Minimal Plates - (reply: 5)
858. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
859. Subcloning Problem - (reply: 3)
860. Restriction Enzyme sites in my genes - (reply: 1)
861. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
862. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
863. In-Fusion (Clontech) Reaction buffer - (reply: 1)
864. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
865. MDA-MB-231 cell transfection - (reply: 5)
866. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
867. Break Cell Clumps - prepare single cells for injections (reply: 1)
868. 126pb insert clonnig/ digestion issues - (reply: 3)
869. cloning problem... - (reply: 5)
870. yeast glycerol stock - (reply: 2)