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Top : New Forum Archives (2009-): : Molecular-Cloning
841. enzymes inactivation - (reply: 3)
842. help: why plasmid yield dropped ever since the first culture - (reply: 2)
843. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
844. miRNA cloning - (reply: 2)
845. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
846. Single-digested vector shifts downward on gel - (reply: 1)
847. problem in digestion - (reply: 1)
848. Cloning does not work - (reply: 4)
849. Small Colonies After Transformation - (reply: 5)
850. Ligation and transformation problem - (reply: 2)
851. Agrobacterium EHA101 competent cells - (reply: 1)
852. restriction digestion against colony PCR - (reply: 1)
853. BAC for transgenic mice - (reply: 6)
854. Tag proteins for CO-IP and confocal - (reply: 1)
855. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
856. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
857. how long for a double digestion - (reply: 2)
858. Problems with Site-Directed Mutagenesis - (reply: 2)
859. blunt and sticky end clonning temperature - (reply: 2)
860. Most common mistakes - What mistakes are made during cloning? (reply: 7)
861. Luciferase Assay Stim. Concentration? - (reply: 1)
862. Cloning direction / orientation? - (reply: 2)
863. Question about plasmid midiprep - (reply: 1)
864. Does a polyA sequence need to be put in-frame? - (reply: 1)
865. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
866. Problem on growing up bugs - (reply: 8)
867. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
868. pSIM in BL21(DE3) - (reply: 2)
869. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
870. Weird problem in invitromutagenesis - (reply: 3)