|
841. Help Miniprep kit SIGMA - (reply: 2)
842. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
843. A question about pBR322 and pUC ori - (reply: 3)
844. Digestion of mini-prep by mistake - (reply: 3)
845. Cloning issue - (reply: 3)
846. Cohesive end filling after CIAP treatment - (reply: 2)
847. Looking for Pfu Taq for high GC-content template - (reply: 7)
848. questions about transformation - (reply: 4)
849. genotype lysogen DE3 - (reply: 1)
850. Need help! - Problem with right insertion! (reply: 1)
851. cloning a large insert - (reply: 5)
852. Transposase contamination in cloning - (reply: 7)
853. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
854. How long can I store cleaved/digested vector? - (reply: 9)
855. Cloning of a gene containing 31 exon - (reply: 1)
856. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
857. Optimum Molar ratio for 3-way ligation - (reply: 5)
858. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
859. cloning help - (reply: 3)
860. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
861. A gene is not inserted into the Plasmid - (reply: 2)
862. Air bubbles during electroporation - (reply: 1)
863. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
864. Colonies without inserts - (reply: 9)
865. Transformants Don't Grow - (reply: 4)
866. control DNA transformation- using pGEM kit - transformation (reply: 4)
867. Topo Cloning - Understanding the principle (reply: 6)
868. pMX-PURO Vector not growing - (reply: 3)
869. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
870. how to set up the ligation - (reply: 14)
842. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
843. A question about pBR322 and pUC ori - (reply: 3)
844. Digestion of mini-prep by mistake - (reply: 3)
845. Cloning issue - (reply: 3)
846. Cohesive end filling after CIAP treatment - (reply: 2)
847. Looking for Pfu Taq for high GC-content template - (reply: 7)
848. questions about transformation - (reply: 4)
849. genotype lysogen DE3 - (reply: 1)
850. Need help! - Problem with right insertion! (reply: 1)
851. cloning a large insert - (reply: 5)
852. Transposase contamination in cloning - (reply: 7)
853. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
854. How long can I store cleaved/digested vector? - (reply: 9)
855. Cloning of a gene containing 31 exon - (reply: 1)
856. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
857. Optimum Molar ratio for 3-way ligation - (reply: 5)
858. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
859. cloning help - (reply: 3)
860. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
861. A gene is not inserted into the Plasmid - (reply: 2)
862. Air bubbles during electroporation - (reply: 1)
863. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
864. Colonies without inserts - (reply: 9)
865. Transformants Don't Grow - (reply: 4)
866. control DNA transformation- using pGEM kit - transformation (reply: 4)
867. Topo Cloning - Understanding the principle (reply: 6)
868. pMX-PURO Vector not growing - (reply: 3)
869. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
870. how to set up the ligation - (reply: 14)