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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
842. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
843. how long for a double digestion - (reply: 2)
844. Problems with Site-Directed Mutagenesis - (reply: 2)
845. blunt and sticky end clonning temperature - (reply: 2)
846. Most common mistakes - What mistakes are made during cloning? (reply: 7)
847. Luciferase Assay Stim. Concentration? - (reply: 1)
848. Cloning direction / orientation? - (reply: 2)
849. Question about plasmid midiprep - (reply: 1)
850. Does a polyA sequence need to be put in-frame? - (reply: 1)
851. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
852. Problem on growing up bugs - (reply: 8)
853. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
854. pSIM in BL21(DE3) - (reply: 2)
855. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
856. Weird problem in invitromutagenesis - (reply: 3)
857. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
858. RE digestion - (reply: 4)
859. HELP!!! - (reply: 3)
860. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
861. Cloning of ITS region - (reply: 6)
862. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
863. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
864. SmaI blunt end ligation problem... - (reply: 3)
865. AAV construct? - (reply: 1)
866. No bands on agarose gel after restriction digestion. - (reply: 10)
867. inverse pcr - (reply: 2)
868. Viral cDNA Library - (reply: 3)
869. metylated cloning vector - restriction digest of the insert (reply: 2)
870. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)