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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
842. Mini prep of frozen bacteria cells - (reply: 2)
843. PCR cloning problem - could not get colony (reply: 4)
844. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
845. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
846. PCR clonning - (reply: 3)
847. 2 bands in blue colony PCR - (reply: 5)
848. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
849. Introduction to vector development - Basics on vectors types and construction (reply: 1)
850. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
851. Targeted mutagenesis using a linear fragment - (reply: 1)
852. Transformation & Minimal Plates - (reply: 5)
853. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
854. Subcloning Problem - (reply: 3)
855. Restriction Enzyme sites in my genes - (reply: 1)
856. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
857. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
858. In-Fusion (Clontech) Reaction buffer - (reply: 1)
859. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
860. MDA-MB-231 cell transfection - (reply: 5)
861. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
862. Break Cell Clumps - prepare single cells for injections (reply: 1)
863. 126pb insert clonnig/ digestion issues - (reply: 3)
864. cloning problem... - (reply: 5)
865. yeast glycerol stock - (reply: 2)
866. yeast transformation - (reply: 1)
867. Problems with ligation and transformation - (reply: 2)
868. very small insert and a very ling vector - (reply: 1)
869. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
870. cDNA clone - (reply: 1)