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Top : New Forum Archives (2009-): : Molecular-Cloning
841. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
842. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
843. SmaI blunt end ligation problem... - (reply: 3)
844. AAV construct? - (reply: 1)
845. No bands on agarose gel after restriction digestion. - (reply: 10)
846. inverse pcr - (reply: 2)
847. Viral cDNA Library - (reply: 3)
848. metylated cloning vector - restriction digest of the insert (reply: 2)
849. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
850. invitro mutagenesis using stragene kit - (reply: 4)
851. Secretion cloning vectors - (reply: 1)
852. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
853. complementing a double knockout in E. coli - (reply: 1)
854. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
855. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
856. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
857. which G418 concentration and treatment period should be used? - (reply: 2)
858. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
859. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
860. Chemical competent E.coli always from single colony ? - (reply: 2)
861. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
862. Strange results of cloning - insert always reverse-oriented (reply: 2)
863. electroporation - (reply: 2)
864. transfection or infection - difference? (reply: 1)
865. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
866. No colonies after transformation! - (reply: 2)
867. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
868. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
869. Help Miniprep kit SIGMA - (reply: 2)
870. Left polymerase above fridge for more than 1 week - still working? (reply: 3)