Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
841. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
842. cDNA clone - (reply: 1)
843. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
844. why these guys use T7 instead of CMV? - for transfection (reply: 2)
845. Electroporation - no colonies (reply: 1)
846. enzymes inactivation - (reply: 3)
847. help: why plasmid yield dropped ever since the first culture - (reply: 2)
848. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
849. miRNA cloning - (reply: 2)
850. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
851. Single-digested vector shifts downward on gel - (reply: 1)
852. problem in digestion - (reply: 1)
853. Cloning does not work - (reply: 4)
854. Small Colonies After Transformation - (reply: 5)
855. Ligation and transformation problem - (reply: 2)
856. Agrobacterium EHA101 competent cells - (reply: 1)
857. restriction digestion against colony PCR - (reply: 1)
858. BAC for transgenic mice - (reply: 6)
859. Tag proteins for CO-IP and confocal - (reply: 1)
860. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
861. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
862. how long for a double digestion - (reply: 2)
863. Problems with Site-Directed Mutagenesis - (reply: 2)
864. blunt and sticky end clonning temperature - (reply: 2)
865. Most common mistakes - What mistakes are made during cloning? (reply: 7)
866. Luciferase Assay Stim. Concentration? - (reply: 1)
867. Cloning direction / orientation? - (reply: 2)
868. Question about plasmid midiprep - (reply: 1)
869. Does a polyA sequence need to be put in-frame? - (reply: 1)
870. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)