Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
841. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
842. In-Fusion (Clontech) Reaction buffer - (reply: 1)
843. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
844. MDA-MB-231 cell transfection - (reply: 5)
845. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
846. Break Cell Clumps - prepare single cells for injections (reply: 1)
847. 126pb insert clonnig/ digestion issues - (reply: 3)
848. cloning problem... - (reply: 5)
849. yeast glycerol stock - (reply: 2)
850. yeast transformation - (reply: 1)
851. Problems with ligation and transformation - (reply: 2)
852. very small insert and a very ling vector - (reply: 1)
853. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
854. cDNA clone - (reply: 1)
855. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
856. why these guys use T7 instead of CMV? - for transfection (reply: 2)
857. Electroporation - no colonies (reply: 1)
858. enzymes inactivation - (reply: 3)
859. help: why plasmid yield dropped ever since the first culture - (reply: 2)
860. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
861. miRNA cloning - (reply: 2)
862. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
863. Single-digested vector shifts downward on gel - (reply: 1)
864. problem in digestion - (reply: 1)
865. Cloning does not work - (reply: 4)
866. Small Colonies After Transformation - (reply: 5)
867. Ligation and transformation problem - (reply: 2)
868. Agrobacterium EHA101 competent cells - (reply: 1)
869. restriction digestion against colony PCR - (reply: 1)
870. BAC for transgenic mice - (reply: 6)