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Top : New Forum Archives (2009-): : Molecular-Cloning
841. blunt and sticky end clonning temperature - (reply: 2)
842. Most common mistakes - What mistakes are made during cloning? (reply: 7)
843. Luciferase Assay Stim. Concentration? - (reply: 1)
844. Cloning direction / orientation? - (reply: 2)
845. Question about plasmid midiprep - (reply: 1)
846. Does a polyA sequence need to be put in-frame? - (reply: 1)
847. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
848. Problem on growing up bugs - (reply: 8)
849. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
850. pSIM in BL21(DE3) - (reply: 2)
851. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
852. Weird problem in invitromutagenesis - (reply: 3)
853. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
854. RE digestion - (reply: 4)
855. HELP!!! - (reply: 3)
856. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
857. Cloning of ITS region - (reply: 6)
858. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
859. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
860. SmaI blunt end ligation problem... - (reply: 3)
861. AAV construct? - (reply: 1)
862. No bands on agarose gel after restriction digestion. - (reply: 10)
863. inverse pcr - (reply: 2)
864. Viral cDNA Library - (reply: 3)
865. metylated cloning vector - restriction digest of the insert (reply: 2)
866. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
867. invitro mutagenesis using stragene kit - (reply: 4)
868. Secretion cloning vectors - (reply: 1)
869. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
870. complementing a double knockout in E. coli - (reply: 1)