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Molecular-Cloning
841.
PCR clonning -
(reply: 3)
842.
2 bands in blue colony PCR -
(reply: 5)
843.
What is the expected pattern of restriction digestion of human genomic DNA? -
(reply: 2)
844.
Introduction to vector development - Basics on vectors types and construction
(reply: 1)
845.
Problems with Geneart Site Directed Mutagenesis -
(reply: 3)
846.
Targeted mutagenesis using a linear fragment -
(reply: 1)
847.
Transformation & Minimal Plates -
(reply: 5)
848.
problems with electroporation of Listeria - please, if you have any ideas..
(reply: 8)
849.
Subcloning Problem -
(reply: 3)
850.
Restriction Enzyme sites in my genes -
(reply: 1)
851.
TOPO vs. Gateway cloning - Which is the better choice for large inserts.
(reply: 4)
852.
Mayday Mayday - about the ligation problems - ligation trouble shooting
(reply: 3)
853.
In-Fusion (Clontech) Reaction buffer -
(reply: 1)
854.
Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor
(reply: 1)
855.
MDA-MB-231 cell transfection -
(reply: 5)
856.
Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar...
(reply: 1)
857.
Break Cell Clumps - prepare single cells for injections
(reply: 1)
858.
126pb insert clonnig/ digestion issues -
(reply: 3)
859.
cloning problem... -
(reply: 5)
860.
yeast glycerol stock -
(reply: 2)
861.
yeast transformation -
(reply: 1)
862.
Problems with ligation and transformation -
(reply: 2)
863.
very small insert and a very ling vector -
(reply: 1)
864.
Guanidine thiocynate contamination in gel extraction - Precipitation vs column
(reply: 1)
865.
cDNA clone -
(reply: 1)
866.
cDNA for plasmid transformation PLEASE HElP -
(reply: 3)
867.
why these guys use T7 instead of CMV? - for transfection
(reply: 2)
868.
Electroporation - no colonies
(reply: 1)
869.
enzymes inactivation -
(reply: 3)
870.
help: why plasmid yield dropped ever since the first culture -
(reply: 2)
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