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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
842. Break Cell Clumps - prepare single cells for injections (reply: 1)
843. 126pb insert clonnig/ digestion issues - (reply: 3)
844. cloning problem... - (reply: 5)
845. yeast glycerol stock - (reply: 2)
846. yeast transformation - (reply: 1)
847. Problems with ligation and transformation - (reply: 2)
848. very small insert and a very ling vector - (reply: 1)
849. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
850. cDNA clone - (reply: 1)
851. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
852. why these guys use T7 instead of CMV? - for transfection (reply: 2)
853. Electroporation - no colonies (reply: 1)
854. enzymes inactivation - (reply: 3)
855. help: why plasmid yield dropped ever since the first culture - (reply: 2)
856. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
857. miRNA cloning - (reply: 2)
858. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
859. Single-digested vector shifts downward on gel - (reply: 1)
860. problem in digestion - (reply: 1)
861. Cloning does not work - (reply: 4)
862. Small Colonies After Transformation - (reply: 5)
863. Ligation and transformation problem - (reply: 2)
864. Agrobacterium EHA101 competent cells - (reply: 1)
865. restriction digestion against colony PCR - (reply: 1)
866. BAC for transgenic mice - (reply: 6)
867. Tag proteins for CO-IP and confocal - (reply: 1)
868. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
869. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
870. how long for a double digestion - (reply: 2)