Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
841. Introduction to vector development - Basics on vectors types and construction (reply: 1)
842. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
843. Targeted mutagenesis using a linear fragment - (reply: 1)
844. Transformation & Minimal Plates - (reply: 5)
845. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
846. Subcloning Problem - (reply: 3)
847. Restriction Enzyme sites in my genes - (reply: 1)
848. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
849. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
850. In-Fusion (Clontech) Reaction buffer - (reply: 1)
851. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
852. MDA-MB-231 cell transfection - (reply: 5)
853. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
854. Break Cell Clumps - prepare single cells for injections (reply: 1)
855. 126pb insert clonnig/ digestion issues - (reply: 3)
856. cloning problem... - (reply: 5)
857. yeast glycerol stock - (reply: 2)
858. yeast transformation - (reply: 1)
859. Problems with ligation and transformation - (reply: 2)
860. very small insert and a very ling vector - (reply: 1)
861. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
862. cDNA clone - (reply: 1)
863. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
864. why these guys use T7 instead of CMV? - for transfection (reply: 2)
865. Electroporation - no colonies (reply: 1)
866. enzymes inactivation - (reply: 3)
867. help: why plasmid yield dropped ever since the first culture - (reply: 2)
868. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
869. miRNA cloning - (reply: 2)
870. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)