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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
842. MDA-MB-231 cell transfection - (reply: 5)
843. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
844. Break Cell Clumps - prepare single cells for injections (reply: 1)
845. 126pb insert clonnig/ digestion issues - (reply: 3)
846. cloning problem... - (reply: 5)
847. yeast glycerol stock - (reply: 2)
848. yeast transformation - (reply: 1)
849. Problems with ligation and transformation - (reply: 2)
850. very small insert and a very ling vector - (reply: 1)
851. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
852. cDNA clone - (reply: 1)
853. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
854. why these guys use T7 instead of CMV? - for transfection (reply: 2)
855. Electroporation - no colonies (reply: 1)
856. enzymes inactivation - (reply: 3)
857. help: why plasmid yield dropped ever since the first culture - (reply: 2)
858. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
859. miRNA cloning - (reply: 2)
860. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
861. Single-digested vector shifts downward on gel - (reply: 1)
862. problem in digestion - (reply: 1)
863. Cloning does not work - (reply: 4)
864. Small Colonies After Transformation - (reply: 5)
865. Ligation and transformation problem - (reply: 2)
866. Agrobacterium EHA101 competent cells - (reply: 1)
867. restriction digestion against colony PCR - (reply: 1)
868. BAC for transgenic mice - (reply: 6)
869. Tag proteins for CO-IP and confocal - (reply: 1)
870. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)