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Top : New Forum Archives (2009-): : Molecular-Cloning
841. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
842. double transformations - (reply: 1)
843. Unusual band in transformants - (reply: 2)
844. ligation--pls help! - (reply: 10)
845. bizarre digestion:) - (reply: 2)
846. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
847. metagenomic library consturction - BAC vectors (reply: 1)
848. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
849. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
850. Chromosomal recombination with BL21? - (reply: 2)
851. Restriction Digestion!! - (reply: 5)
852. digestion - restriction enzymes (reply: 2)
853. How to prevent self ligation - selfligation (reply: 11)
854. what is the difference between DH10B and Top10 - (reply: 1)
855. problems encountered when working on a large plasmid - (reply: 2)
856. make a Mega plasmid mutation - (reply: 1)
857. Cloning - (reply: 1)
858. Copy-number calculation formula? - (reply: 1)
859. pRL-CMV vector - 2500nt insert. Is it possible? (reply: 2)
860. low melt agarose - (reply: 1)
861. restriction digest - smear - (reply: 1)
862. designing of primers - (reply: 2)
863. Propagating ccdB survival cells - (reply: 6)
864. Bicistronic vector vs "normal" vector - (reply: 5)
865. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
866. Can't seem to get insert into vector... please help - (reply: 4)
867. plasmid cloning - i need help.. can anyone answer me (reply: 5)
868. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
869. Topo cloning or pGEM-T easy ? - (reply: 3)
870. Problems with qiaquick gel purification kit - cloning (reply: 10)