Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
841. cloning a large insert - (reply: 5)
842. Transposase contamination in cloning - (reply: 7)
843. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
844. How long can I store cleaved/digested vector? - (reply: 9)
845. Cloning of a gene containing 31 exon - (reply: 1)
846. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
847. Optimum Molar ratio for 3-way ligation - (reply: 5)
848. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
849. cloning help - (reply: 3)
850. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
851. A gene is not inserted into the Plasmid - (reply: 2)
852. Air bubbles during electroporation - (reply: 1)
853. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
854. Colonies without inserts - (reply: 9)
855. Transformants Don't Grow - (reply: 4)
856. control DNA transformation- using pGEM kit - transformation (reply: 4)
857. Topo Cloning - Understanding the principle (reply: 6)
858. pMX-PURO Vector not growing - (reply: 3)
859. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
860. how to set up the ligation - (reply: 14)
861. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
862. DH5 Alpha Transformation - (reply: 2)
863. Cloning a foreign gene into E-coli - (reply: 1)
864. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
865. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
866. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
867. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
868. restriction digest simple question - (reply: 4)
869. ligating small inert into a large vector - (reply: 4)
870. tranformation trouble using dh5alpha - (reply: 1)