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841. Screening colonies after transformation - (reply: 2)
842. z competent cells - (reply: 2)
843. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
844. insert release not as intense as the plasmid - is it always like that? (reply: 3)
845. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
846. plasmid extraction kit wash buffer solution - (reply: 3)
847. molecular "terminology" - (reply: 1)
848. NdeI digestion - Share your experience (reply: 3)
849. Multiple bands after amping insert - (reply: 2)
850. Good tool for sequence validation? - For Windows (reply: 2)
851. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
852. Silencing of the first cistron - (reply: 1)
853. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
854. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
855. Stable Mammalian Expression with Retrovirus - (reply: 2)
856. Neon Transfection System - (reply: 2)
857. help - (reply: 5)
858. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
859. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
860. problems with qiagen gel extraction kit - (reply: 7)
861. restriction enzyme flanking sequence preferance - (reply: 2)
862. pCDH Lentivector has 500 bp insert - (reply: 5)
863. 5' RACE for very GC-rich gene - (reply: 2)
864. Phosphorylated primers - (reply: 5)
865. Correct orientation of the insert - (reply: 7)
866. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
867. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
868. Cloning advice needed - (reply: 4)
869. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
870. Colony Screeing without using PCR - (reply: 8)
842. z competent cells - (reply: 2)
843. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
844. insert release not as intense as the plasmid - is it always like that? (reply: 3)
845. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
846. plasmid extraction kit wash buffer solution - (reply: 3)
847. molecular "terminology" - (reply: 1)
848. NdeI digestion - Share your experience (reply: 3)
849. Multiple bands after amping insert - (reply: 2)
850. Good tool for sequence validation? - For Windows (reply: 2)
851. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
852. Silencing of the first cistron - (reply: 1)
853. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
854. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
855. Stable Mammalian Expression with Retrovirus - (reply: 2)
856. Neon Transfection System - (reply: 2)
857. help - (reply: 5)
858. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
859. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
860. problems with qiagen gel extraction kit - (reply: 7)
861. restriction enzyme flanking sequence preferance - (reply: 2)
862. pCDH Lentivector has 500 bp insert - (reply: 5)
863. 5' RACE for very GC-rich gene - (reply: 2)
864. Phosphorylated primers - (reply: 5)
865. Correct orientation of the insert - (reply: 7)
866. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
867. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
868. Cloning advice needed - (reply: 4)
869. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
870. Colony Screeing without using PCR - (reply: 8)