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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
842. DH5 Alpha Transformation - (reply: 2)
843. Cloning a foreign gene into E-coli - (reply: 1)
844. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
845. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
846. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
847. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
848. restriction digest simple question - (reply: 4)
849. ligating small inert into a large vector - (reply: 4)
850. tranformation trouble using dh5alpha - (reply: 1)
851. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
852. Starting cells for making one's own competent cells - (reply: 7)
853. 6 months... and still no positive clones! - (reply: 4)
854. Really strange problem with sequencing and plasmid purification! - (reply: 8)
855. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
856. what is site-specific integration - (reply: 2)
857. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
858. double transformations - (reply: 1)
859. Unusual band in transformants - (reply: 2)
860. ligation--pls help! - (reply: 10)
861. bizarre digestion:) - (reply: 2)
862. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
863. metagenomic library consturction - BAC vectors (reply: 1)
864. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
865. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
866. Chromosomal recombination with BL21? - (reply: 2)
867. Restriction Digestion!! - (reply: 5)
868. digestion - restriction enzymes (reply: 2)
869. How to prevent self ligation - selfligation (reply: 11)
870. what is the difference between DH10B and Top10 - (reply: 1)