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841. Questions about pIRES-hrGFP1a - (reply: 1)
842. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
843. Cloning into lentiviral vector - (reply: 11)
844. cloning failure help plzzz - cloning (reply: 1)
845. use of pGEM -tT easy vectors - (reply: 2)
846. ~200kb Plasmid DNA purification is not working - (reply: 1)
847. ligating a 6 His tag to plasmid - (reply: 2)
848. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
849. ligation and transformation problem - (reply: 1)
850. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
851. Mini prep of frozen bacteria cells - (reply: 2)
852. PCR cloning problem - could not get colony (reply: 4)
853. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
854. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
855. PCR clonning - (reply: 3)
856. 2 bands in blue colony PCR - (reply: 5)
857. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
858. Introduction to vector development - Basics on vectors types and construction (reply: 1)
859. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
860. Targeted mutagenesis using a linear fragment - (reply: 1)
861. Transformation & Minimal Plates - (reply: 5)
862. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
863. Subcloning Problem - (reply: 3)
864. Restriction Enzyme sites in my genes - (reply: 1)
865. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
866. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
867. In-Fusion (Clontech) Reaction buffer - (reply: 1)
868. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
869. MDA-MB-231 cell transfection - (reply: 5)
870. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
842. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
843. Cloning into lentiviral vector - (reply: 11)
844. cloning failure help plzzz - cloning (reply: 1)
845. use of pGEM -tT easy vectors - (reply: 2)
846. ~200kb Plasmid DNA purification is not working - (reply: 1)
847. ligating a 6 His tag to plasmid - (reply: 2)
848. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
849. ligation and transformation problem - (reply: 1)
850. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
851. Mini prep of frozen bacteria cells - (reply: 2)
852. PCR cloning problem - could not get colony (reply: 4)
853. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
854. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
855. PCR clonning - (reply: 3)
856. 2 bands in blue colony PCR - (reply: 5)
857. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
858. Introduction to vector development - Basics on vectors types and construction (reply: 1)
859. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
860. Targeted mutagenesis using a linear fragment - (reply: 1)
861. Transformation & Minimal Plates - (reply: 5)
862. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
863. Subcloning Problem - (reply: 3)
864. Restriction Enzyme sites in my genes - (reply: 1)
865. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
866. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
867. In-Fusion (Clontech) Reaction buffer - (reply: 1)
868. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
869. MDA-MB-231 cell transfection - (reply: 5)
870. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)