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Top : New Forum Archives (2009-): : Molecular-Cloning
841. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
842. Introduction to vector development - Basics on vectors types and construction (reply: 1)
843. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
844. Targeted mutagenesis using a linear fragment - (reply: 1)
845. Transformation & Minimal Plates - (reply: 5)
846. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
847. Subcloning Problem - (reply: 3)
848. Restriction Enzyme sites in my genes - (reply: 1)
849. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
850. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
851. In-Fusion (Clontech) Reaction buffer - (reply: 1)
852. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
853. MDA-MB-231 cell transfection - (reply: 5)
854. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
855. Break Cell Clumps - prepare single cells for injections (reply: 1)
856. 126pb insert clonnig/ digestion issues - (reply: 3)
857. cloning problem... - (reply: 5)
858. yeast glycerol stock - (reply: 2)
859. yeast transformation - (reply: 1)
860. Problems with ligation and transformation - (reply: 2)
861. very small insert and a very ling vector - (reply: 1)
862. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
863. cDNA clone - (reply: 1)
864. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
865. why these guys use T7 instead of CMV? - for transfection (reply: 2)
866. Electroporation - no colonies (reply: 1)
867. enzymes inactivation - (reply: 3)
868. help: why plasmid yield dropped ever since the first culture - (reply: 2)
869. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
870. miRNA cloning - (reply: 2)