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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Secretion cloning vectors - (reply: 1)
842. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
843. complementing a double knockout in E. coli - (reply: 1)
844. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
845. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
846. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
847. which G418 concentration and treatment period should be used? - (reply: 2)
848. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
849. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
850. Chemical competent E.coli always from single colony ? - (reply: 2)
851. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
852. Strange results of cloning - insert always reverse-oriented (reply: 2)
853. electroporation - (reply: 2)
854. transfection or infection - difference? (reply: 1)
855. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
856. No colonies after transformation! - (reply: 2)
857. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
858. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
859. Help Miniprep kit SIGMA - (reply: 2)
860. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
861. A question about pBR322 and pUC ori - (reply: 3)
862. Digestion of mini-prep by mistake - (reply: 3)
863. Cloning issue - (reply: 3)
864. Cohesive end filling after CIAP treatment - (reply: 2)
865. Looking for Pfu Taq for high GC-content template - (reply: 7)
866. questions about transformation - (reply: 4)
867. genotype lysogen DE3 - (reply: 1)
868. Need help! - Problem with right insertion! (reply: 1)
869. cloning a large insert - (reply: 5)
870. Transposase contamination in cloning - (reply: 7)