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Top : New Forum Archives (2009-): : Molecular-Cloning
841. Restriction Enzyme sites in my genes - (reply: 1)
842. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
843. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
844. In-Fusion (Clontech) Reaction buffer - (reply: 1)
845. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
846. MDA-MB-231 cell transfection - (reply: 5)
847. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
848. Break Cell Clumps - prepare single cells for injections (reply: 1)
849. 126pb insert clonnig/ digestion issues - (reply: 3)
850. cloning problem... - (reply: 5)
851. yeast glycerol stock - (reply: 2)
852. yeast transformation - (reply: 1)
853. Problems with ligation and transformation - (reply: 2)
854. very small insert and a very ling vector - (reply: 1)
855. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
856. cDNA clone - (reply: 1)
857. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
858. why these guys use T7 instead of CMV? - for transfection (reply: 2)
859. Electroporation - no colonies (reply: 1)
860. enzymes inactivation - (reply: 3)
861. help: why plasmid yield dropped ever since the first culture - (reply: 2)
862. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
863. miRNA cloning - (reply: 2)
864. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
865. Single-digested vector shifts downward on gel - (reply: 1)
866. problem in digestion - (reply: 1)
867. Cloning does not work - (reply: 4)
868. Small Colonies After Transformation - (reply: 5)
869. Ligation and transformation problem - (reply: 2)
870. Agrobacterium EHA101 competent cells - (reply: 1)