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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. Topo directional cloning - pENTR/D-TOPO (reply: 1)
1022. trouble in TOPO TA sequencing - (reply: 1)
1023. Large DNA extraction from an agarose gel - (reply: 8)
1024. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1025. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1026. Ligation problem, religation of backbone without any insert? - (reply: 3)
1027. problem with pLK01 plasmid ligation - (reply: 2)
1028. Mini prep streaking - basic question (reply: 3)
1029. In-fusion joining few parts together - possible? ideas? (reply: 3)
1030. TOPO cloning problem - (reply: 4)
1031. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1032. Red/ET recombination - (reply: 2)
1033. multiple origins of replication - (reply: 2)
1034. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1035. Single Restriction Site cloning - Cloning (reply: 6)
1036. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1037. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1038. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1039. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1040. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1041. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1042. Agarose gel electrophoresis question - (reply: 1)
1043. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1044. My white colonies turned blue in frig! Why? - (reply: 6)
1045. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1046. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1047. Dephosphorylation of vector - (reply: 8)
1048. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1049. rna isolation - (reply: 1)
1050. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)