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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1022. rna isolation - (reply: 1)
1023. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
1024. Molecular Cloning od Recombinant DNA - (reply: 3)
1025. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
1026. looking for binding site - software? (reply: 1)
1027. Insertion of loxp/avoiding SINE - (reply: 1)
1028. Question: Ligation Problem.... - (reply: 2)
1029. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
1030. Topo directional cloning - pENTR/D-TOPO (reply: 1)
1031. trouble in TOPO TA sequencing - (reply: 1)
1032. Large DNA extraction from an agarose gel - (reply: 8)
1033. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1034. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1035. Ligation problem, religation of backbone without any insert? - (reply: 3)
1036. problem with pLK01 plasmid ligation - (reply: 2)
1037. Mini prep streaking - basic question (reply: 3)
1038. In-fusion joining few parts together - possible? ideas? (reply: 3)
1039. TOPO cloning problem - (reply: 4)
1040. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1041. Red/ET recombination - (reply: 2)
1042. multiple origins of replication - (reply: 2)
1043. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1044. Single Restriction Site cloning - Cloning (reply: 6)
1045. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1046. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1047. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1048. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1049. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1050. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)