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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. TOPO cloning problem - (reply: 4)
1022. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1023. Red/ET recombination - (reply: 2)
1024. multiple origins of replication - (reply: 2)
1025. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1026. Single Restriction Site cloning - Cloning (reply: 6)
1027. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1028. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1029. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1030. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
1031. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
1032. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
1033. Agarose gel electrophoresis question - (reply: 1)
1034. amount of plasmid for transformation and fusion protein expression - (reply: 1)
1035. My white colonies turned blue in frig! Why? - (reply: 6)
1036. Digestion problem XbaI, methylation sensitive site - (reply: 3)
1037. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
1038. Dephosphorylation of vector - (reply: 8)
1039. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
1040. rna isolation - (reply: 1)
1041. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1042. So many colonies! - (reply: 8)
1043. Large vector cloning-recombination problem? - (reply: 1)
1044. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1045. selecting recombinants - (reply: 1)
1046. colony PCR for subcloning gene - (reply: 1)
1047. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1048. cloning of a 20 kb insert - (reply: 2)
1049. DNA quantification after gel purification - (reply: 6)
1050. cloning of an operon - (reply: 2)