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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1022. Plasmid out of BL21 for mutagensis - (reply: 3)
1023. no colony growth - (reply: 4)
1024. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1025. Effect of a DNA prep on DE3 - (reply: 2)
1026. Competent cell storage for long term use - (reply: 2)
1027. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1028. New to sequencing, primer design - (reply: 2)
1029. ta cloning problem - (reply: 2)
1030. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1031. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
1032. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1033. Storage of Bacterial Culture at 4 degrees - (reply: 2)
1034. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1035. rna isolation - (reply: 1)
1036. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
1037. Molecular Cloning od Recombinant DNA - (reply: 3)
1038. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
1039. looking for binding site - software? (reply: 1)
1040. Insertion of loxp/avoiding SINE - (reply: 1)
1041. Question: Ligation Problem.... - (reply: 2)
1042. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
1043. Topo directional cloning - pENTR/D-TOPO (reply: 1)
1044. trouble in TOPO TA sequencing - (reply: 1)
1045. Large DNA extraction from an agarose gel - (reply: 8)
1046. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1047. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1048. Ligation problem, religation of backbone without any insert? - (reply: 3)
1049. problem with pLK01 plasmid ligation - (reply: 2)
1050. Mini prep streaking - basic question (reply: 3)