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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
1022. ligation - (reply: 1)
1023. pre-prepararation of competent cells - (reply: 3)
1024. different copy # multimers with linker ligation? - (reply: 2)
1025. Help ADD 3 bases to DNA Sequences - (reply: 6)
1026. My battle with BamHI - (reply: 27)
1027. serious problem in cloning - (reply: 2)
1028. What are "Ella-cre" mice? - (reply: 2)
1029. help with pCMV-myc vector - (reply: 2)
1030. Elution amount - (reply: 5)
1031. keeping restriction digested fragments at -20? - (reply: 5)
1032. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
1033. re-digestion - (reply: 1)
1034. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
1035. Cloning using BamH1 and HindIII - (reply: 8)
1036. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
1037. "competentcy" of my method - (reply: 5)
1038. Vector frameshift problem - (reply: 3)
1039. Screening colonies after transformation - (reply: 2)
1040. z competent cells - (reply: 2)
1041. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
1042. insert release not as intense as the plasmid - is it always like that? (reply: 3)
1043. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
1044. plasmid extraction kit wash buffer solution - (reply: 3)
1045. molecular "terminology" - (reply: 1)
1046. NdeI digestion - Share your experience (reply: 3)
1047. Multiple bands after amping insert - (reply: 2)
1048. Good tool for sequence validation? - For Windows (reply: 2)
1049. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
1050. Silencing of the first cistron - (reply: 1)