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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1022. Storage of Bacterial Culture at 4 degrees - (reply: 2)
1023. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1024. rna isolation - (reply: 1)
1025. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
1026. Molecular Cloning od Recombinant DNA - (reply: 3)
1027. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
1028. looking for binding site - software? (reply: 1)
1029. Insertion of loxp/avoiding SINE - (reply: 1)
1030. Question: Ligation Problem.... - (reply: 2)
1031. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
1032. Topo directional cloning - pENTR/D-TOPO (reply: 1)
1033. trouble in TOPO TA sequencing - (reply: 1)
1034. Large DNA extraction from an agarose gel - (reply: 8)
1035. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
1036. primer Tm is too high, how tu get pcr product - help! (reply: 21)
1037. Ligation problem, religation of backbone without any insert? - (reply: 3)
1038. problem with pLK01 plasmid ligation - (reply: 2)
1039. Mini prep streaking - basic question (reply: 3)
1040. In-fusion joining few parts together - possible? ideas? (reply: 3)
1041. TOPO cloning problem - (reply: 4)
1042. How to delete a domain in a gene? - cloned plasmid (reply: 12)
1043. Red/ET recombination - (reply: 2)
1044. multiple origins of replication - (reply: 2)
1045. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
1046. Single Restriction Site cloning - Cloning (reply: 6)
1047. LR Reaction - Unwanted Homologous Recombinations - (reply: 1)
1048. 3-(N-morpholino)propanesulfonic acid: MOPS - (reply: 6)
1049. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
1050. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)