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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. rna isolation - (reply: 1)
1022. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
1023. So many colonies! - (reply: 8)
1024. Large vector cloning-recombination problem? - (reply: 1)
1025. dose incubation in 16 C increase the ligation efficiebcy? - (reply: 10)
1026. selecting recombinants - (reply: 1)
1027. colony PCR for subcloning gene - (reply: 1)
1028. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
1029. cloning of a 20 kb insert - (reply: 2)
1030. DNA quantification after gel purification - (reply: 6)
1031. cloning of an operon - (reply: 2)
1032. storing plasmid - -80degrees (reply: 4)
1033. verifying a positive transformant - (reply: 3)
1034. Need advice on cloning very small insert into a vector - (reply: 3)
1035. Cloning problems i.e. Ligation - (reply: 2)
1036. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
1037. Radioactive carbon - (reply: 3)
1038. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
1039. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
1040. Digesting large amount of plasmid - (reply: 4)
1041. cleanup of digestion before ligation - (reply: 6)
1042. transformation efficiency - (reply: 7)
1043. transformed bacteria doesnt grow - (reply: 1)
1044. TOPO Cloning - (reply: 5)
1045. blunt ligation - (reply: 4)
1046. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
1047. About Laminin 5'UTR 's function - (reply: 1)
1048. Vectors with special MCS - (reply: 5)
1049. Cloning <100 bp fragments into a vector - (reply: 7)
1050. Help! my plasmid size changed after ligation - (reply: 5)