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Top : New Forum Archives (2009-): : Molecular-Cloning
1021. Help cloning large insert into large vector - (reply: 7)
1022. problem about pIRES2-DsRed2 double digestion - (reply: 2)
1023. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
1024. hENT1 - (reply: 1)
1025. digestion of insert twice and ligation of 3 bits - (reply: 6)
1026. blunt end ligation problem - Self ligation and truncate (reply: 1)
1027. Dephosphorylation of insert - Dephosphorylation of insert (reply: 3)
1028. neb ligase - (reply: 2)
1029. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
1030. problem about vector Dephosphorylation - (reply: 2)
1031. Early transfection - (reply: 6)
1032. Cloning: Randomly recombined Plasmids? - (reply: 6)
1033. problem about fused protein - problem about fused protein (reply: 2)
1034. Bacteria growing on plate but not liquid media - (reply: 8)
1035. What means BamHI-linkered - (reply: 1)
1036. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
1037. Problems with Dpn1 - (reply: 5)
1038. forgot to flip the plate over - but still got colonies (reply: 2)
1039. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
1040. Yet another ligation problem - (reply: 6)
1041. Cloning problems - (reply: 7)
1042. Bacteria and Amaxa - (reply: 2)
1043. Problem in Ligation - (reply: 12)
1044. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
1045. multiway ligation - (reply: 6)
1046. Plasmid size problems! - (reply: 3)
1047. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
1048. ligation problem - (reply: 6)
1049. Failed Ligation - (reply: 2)
1050. cloning of GroEL - (reply: 3)