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1021. Propagating ccdB survival cells - (reply: 6)
1022. Bicistronic vector vs "normal" vector - (reply: 5)
1023. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1024. Can't seem to get insert into vector... please help - (reply: 4)
1025. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1026. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1027. Topo cloning or pGEM-T easy ? - (reply: 3)
1028. Problems with qiaquick gel purification kit - cloning (reply: 10)
1029. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1030. integration of a gene per homologous recombination - (reply: 5)
1031. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1032. Antarctic Phosphatase treatment - (reply: 5)
1033. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1034. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1035. Plasmid out of BL21 for mutagensis - (reply: 3)
1036. no colony growth - (reply: 4)
1037. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1038. Effect of a DNA prep on DE3 - (reply: 2)
1039. Competent cell storage for long term use - (reply: 2)
1040. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1041. New to sequencing, primer design - (reply: 2)
1042. ta cloning problem - (reply: 2)
1043. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1044. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
1045. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1046. Storage of Bacterial Culture at 4 degrees - (reply: 2)
1047. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1048. rna isolation - (reply: 1)
1049. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
1050. Molecular Cloning od Recombinant DNA - (reply: 3)
1022. Bicistronic vector vs "normal" vector - (reply: 5)
1023. Insert much smaller than expected after miniprep - DH5a is eating my insert! (reply: 6)
1024. Can't seem to get insert into vector... please help - (reply: 4)
1025. plasmid cloning - i need help.. can anyone answer me (reply: 5)
1026. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
1027. Topo cloning or pGEM-T easy ? - (reply: 3)
1028. Problems with qiaquick gel purification kit - cloning (reply: 10)
1029. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
1030. integration of a gene per homologous recombination - (reply: 5)
1031. Site Directed Mutagenesis - Stratagene's Quickchange II XL Protocol - Oligo Issues - Melting Temperature (reply: 1)
1032. Antarctic Phosphatase treatment - (reply: 5)
1033. same promoter for insert and resistance?? - need a new promoter for zeocin resistance (reply: 1)
1034. phage DNA - DNA wont migrate on agarose gel (reply: 3)
1035. Plasmid out of BL21 for mutagensis - (reply: 3)
1036. no colony growth - (reply: 4)
1037. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
1038. Effect of a DNA prep on DE3 - (reply: 2)
1039. Competent cell storage for long term use - (reply: 2)
1040. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
1041. New to sequencing, primer design - (reply: 2)
1042. ta cloning problem - (reply: 2)
1043. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
1044. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
1045. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
1046. Storage of Bacterial Culture at 4 degrees - (reply: 2)
1047. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
1048. rna isolation - (reply: 1)
1049. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
1050. Molecular Cloning od Recombinant DNA - (reply: 3)