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Top : New Forum Archives (2009-): : Molecular-Cloning
1351. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1352. cloning help - (reply: 9)
1353. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1354. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1355. Ligation trouble continues - subcloning ligation failure (reply: 9)
1356. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1357. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1358. colony PCR - (reply: 8)
1359. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1360. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1361. Truncated sequence - (reply: 1)
1362. gateway cloning - (reply: 3)
1363. Purifying and cloning 69nt product - (reply: 5)
1364. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
1365. ligatin issues - (reply: 1)
1366. Chloramphenicol concentration in M9 minimal media - What is maniatis and where can I find it? lol (reply: 1)
1367. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
1368. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
1369. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
1370. A gateway cloning expression vector question - (reply: 1)
1371. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
1372. Promoter/gene cloning question - (reply: 7)
1373. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
1374. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
1375. vivo expression technology - ivet (reply: 25)
1376. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
1377. insert is missing after TA cloning - TA cloning (reply: 6)
1378. glycerol stock - shelf life (reply: 5)
1379. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1380. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)