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Top : New Forum Archives (2009-): : Molecular-Cloning
1351. mutagenesis expert come in~ - (reply: 11)
1352. Subcloning - (reply: 3)
1353. Sequencing different colonies - (reply: 5)
1354. detection of restriction? - (reply: 1)
1355. problem in pcr and purification - (reply: 3)
1356. bacterial stock preparation - (reply: 3)
1357. Problem with site directed mutagenesis - (reply: 7)
1358. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1359. Forgot to add X-gal/IPTG - (reply: 8)
1360. after re-isolation of vector, get single band - (reply: 4)
1361. Subcloning into pMIR-REPORT-luc - (reply: 7)
1362. Restriction digestion troubleshooting - (reply: 10)
1363. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1364. Which digestion enzyme? - (reply: 1)
1365. Cloning big fragment into smaller vector - (reply: 3)
1366. from where can i get the P1 phage - (reply: 1)
1367. multi way ligation or long range pcr? - (reply: 1)
1368. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1369. list of sequence to be added before RE site - (reply: 3)
1370. plasmid, BAC, or cosmid? - (reply: 3)
1371. Wierd trouble - dunno where my insert is?? (reply: 3)
1372. Large Molecular weight DNA Extarction - (reply: 7)
1373. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1374. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1375. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1376. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1377. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1378. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1379. Vector sequence included in (PCR) amplified insert - (reply: 2)
1380. COMPETENT CELL PREPARATION - (reply: 6)