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Top : New Forum Archives (2009-): : Molecular-Cloning
1351. DNA yield estimation - (reply: 1)
1352. Vector NTI from Invitrogen - (reply: 4)
1353. gfp-tagged protein not expressing- help! - (reply: 3)
1354. anyone working currently on gateway cloning???? - (reply: 1)
1355. Dpn1 enzyme - (reply: 2)
1356. mutagenesis expert come in~ - (reply: 11)
1357. Subcloning - (reply: 3)
1358. Sequencing different colonies - (reply: 5)
1359. detection of restriction? - (reply: 1)
1360. problem in pcr and purification - (reply: 3)
1361. bacterial stock preparation - (reply: 3)
1362. Problem with site directed mutagenesis - (reply: 7)
1363. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
1364. Forgot to add X-gal/IPTG - (reply: 8)
1365. after re-isolation of vector, get single band - (reply: 4)
1366. Subcloning into pMIR-REPORT-luc - (reply: 7)
1367. Restriction digestion troubleshooting - (reply: 10)
1368. Problems with Quick change lightning multi site directed mutagenesis kit - (reply: 10)
1369. Which digestion enzyme? - (reply: 1)
1370. Cloning big fragment into smaller vector - (reply: 3)
1371. from where can i get the P1 phage - (reply: 1)
1372. multi way ligation or long range pcr? - (reply: 1)
1373. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1374. list of sequence to be added before RE site - (reply: 3)
1375. plasmid, BAC, or cosmid? - (reply: 3)
1376. Wierd trouble - dunno where my insert is?? (reply: 3)
1377. Large Molecular weight DNA Extarction - (reply: 7)
1378. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1379. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1380. Difficulty cloning vector with multiple Lox sites - (reply: 2)