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Top : New Forum Archives (2009-): : Molecular-Cloning
1351. glycerol stock - shelf life (reply: 5)
1352. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
1353. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
1354. cloning problem - molecular cloning (reply: 1)
1355. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
1356. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
1357. Gemonic Library - Deletion/Reduction of Chloro/Mit DNA (reply: 1)
1358. choosing restriction sites - (reply: 5)
1359. Tag insertion - (reply: 5)
1360. Transduction - (reply: 3)
1361. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
1362. my colonies don't have the insert, how is this possible? - (reply: 7)
1363. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
1364. PCR problem from transformed TOPO TA vector - (reply: 3)
1365. nondenaturing PAGE to separate dna - (reply: 4)
1366. why sometimes big or small colonies? - (reply: 16)
1367. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
1368. wrong bands after T-vector cloning - (reply: 3)
1369. urgent help regarding wanner method trouble shooting - (reply: 2)
1370. two step digestion - (reply: 6)
1371. Storage of invitrogen top10 cells - (reply: 11)
1372. Self-Ligation of digested vector - (reply: 2)
1373. Help with cloning - (reply: 6)
1374. Ligation issue: Does vector size matter? - (reply: 10)
1375. Help for T-A cloning - (reply: 15)
1376. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
1377. Some colonies, but not enough... - (reply: 7)
1378. transformation problem - (reply: 3)
1379. Plasmid sequence - (reply: 1)
1380. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)