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Top : New Forum Archives (2009-): : Molecular-Cloning
1351. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1352. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1353. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1354. T A cloning problems - (reply: 3)
1355. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1356. Maximum recovery time after electroporation - (reply: 2)
1357. pCLBABE puro retriviral vector - (reply: 1)
1358. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1359. Blunt-end cloning problem - (reply: 6)
1360. Supercoiling blocking restriction site? - (reply: 6)
1361. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1362. problem in gene cloning - (reply: 4)
1363. Odd point mutations - (reply: 23)
1364. Plasmid linearization by PCR - (reply: 4)
1365. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
1366. can't make the cloning work - (reply: 3)
1367. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
1368. cloning help - (reply: 9)
1369. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
1370. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
1371. Ligation trouble continues - subcloning ligation failure (reply: 9)
1372. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
1373. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
1374. colony PCR - (reply: 8)
1375. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
1376. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
1377. Truncated sequence - (reply: 1)
1378. gateway cloning - (reply: 3)
1379. Purifying and cloning 69nt product - (reply: 5)
1380. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)